36 results about "Polysaccharide degradation" patented technology

The invention relates to the field of traditional Chinese medicines, and discloses a preparation method and application of a pectic polysaccharide degradation product of an overground part of radix tetrastigme. The preparation method comprises the following steps: 1) extracting radix tetrastigme crude polysaccharide in the overground part of radix tetrastigme; 2) dialyzing the radix tetrastigme crude polysaccharide, and performing gradient elution through a cellulose column to obtain an eluent; (3) eluting with gel column water to obtain radix tetrastigme pectic polysaccharide; (4) carrying out enzymolysis on the radix tetrastigme pectic polysaccharide by using pectinase; and eluting with a gel column to obtain a degradation product of the tetrastigma hemsleyanum pectic polysaccharide. Compared with a product before enzymolysis, the pectic polysaccharide degradation product of the aboveground part of the radix tetrastigme, which is prepared by the method disclosed by the invention, has more advantages in the aspect of immune activation effect, and is beneficial to the development and application of the aboveground part of the radix tetrastigme in the fields of foods and medicines.

The invention relates to the technical field of oligosaccharides, in particular to yam bean oligosaccharides, a preparation method thereof and application of the yam bean oligosaccharides in preparation of products for preventing or treating ulcerative colitis. The method comprises the following steps: mixing yam bean polysaccharide with a ferrous ion solution, adjusting the pH value to 2.0-6.0, adding a hydrogen peroxide solution, and carrying out ultrasonic treatment in an ultrasonic cell crusher to obtain yam bean polysaccharide degradation liquid; mixing the yam bean polysaccharide degradation liquid with a sodium hydroxide solution, removing solid substances and hydrogen peroxide, and purifying and drying to obtain yam bean oligosaccharide. The yam bean oligosaccharide can be used for preparing related products for preventing or treating ulcerative colitis.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

A method for preparing a roasted food product, suitable for preparing a coffee-like 5 beverage product, starting from one or more pulses, comprising one or more pretreatment steps followed by one or more roasting steps, wherein preferably the roasting temperature is in the range of 75 °C to 250 °C, more preferably in the range of 125 °C to 225 °C, even more preferably in the range of 150 °C to 200 °C, wherein the pretreatment comprises the following steps: ▪ at least one polysaccharide degradation step, preferably for starch degradation; ▪ at least one acidification step, preferably by fermentation, to obtain a pH preferably below 6 of the roasted food product, more preferably below 5.5, most preferably below 5; ▪ at least one drying step, wherein the water content of the pulses obtained by the polysaccharide degradation and acidification steps is adjusted to preferably 14 weight% or less, more preferably to 12 weight%, most preferably to 10 weight% as compared to the total weight of the pretreated pulses.

The application discloses a tremella aurantialba polysaccharide degradation product, which is prepared by degrading tremella aurantialba polysaccharide by a method of combining H2O2 and vitamin C and selecting degradation products with a molecular weight greater than 100 k Da and a molecular weight of 1 k Da to 100 k Da. Effective degradation of the tremella aurantialba polysaccharide can greatly improve the bioavailability of the tremella aurantialba polysaccharide, and the tremella aurantialba polysaccharide degradation product I (E-TAP I) and the tremella aurantialba polysaccharide degradation product II (E-TAP II) have good application prospects in skin thermal injury repair.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.

The invention provides a preparation method, relates to the technical field of deep processing of rhizoma polygonati, and particularly discloses a preparation method of rhizoma polygonati active peptide with homology of medicine and food. The preparation method comprises the following steps: slicing, preparing water, cooking, degrading polysaccharide, carrying out targeted enzyme digestion, carrying out solid-liquid separation, deactivating enzyme, adding monomethoxy polyethylene glycol, carrying out combined modification, carrying out centrifugal purification, carrying out nanofiltration to remove polyethylene glycol, concentrating, and carrying out spray drying on the obtained concentrated solution to obtain a medicinal and edible polygonatum kingianum active peptide product. The preparation method is short in production period, low in cost and free of environmental pollution, and the obtained product is high in safety, high in purity and the like and can be widely applied to the aspects of food, health care products, special medical food, medicine and the like.

The application provides a biological skin activity fermentation liquor with multiple effects, a preparation method thereof and a cosmetic, and relates to the cosmetic field.The method comprises the following steps: mixing camellia oleifera seed meal, protease, cellulase, lipase and water to obtain a first mixture; performing enzymolysis on the first mixture to obtain a fermentation substrate; performing second mixing on the fermentation substrate, glucose, peptone, yeast powder, disodium hydrogen phosphate and citric acid, and sterilizing to obtain a fermentation culture medium; activating bacillus velezensis through an LB culture medium to obtain a seed liquid; performing third mixing on the seed liquid and the fermentation culture medium, and performing fermentation to obtain a camellia oleifera seed meal fermentation liquor; performing centrifugation on the camellia oleifera seed meal fermentation liquor to obtain a centrifuged fermentation liquor; performing fourth mixing on the centrifuged fermentation liquor and polysaccharide-degrading enzyme, performing enzymolysis, and performing filtration to obtain the biological skin activity fermentation liquor with multiple effects.The method not only improves the high-value utilization of the camellia oleifera seed meal, but also obtains the biological skin activity fermentation liquor with high safety and outstanding activity.

The application discloses a kind of Amomum tsao-ko degradation polysaccharides with neuroprotective activity and a preparation method thereof, and specifically relates to the technical field of Amomum tsao-ko polysaccharide degradation.The preparation method uses a degrading agent H2O2-Vc to be mixed with 1-5 mg / mL Amomum tsao-ko polysaccharide solution until the final concentration of H2O2-Vc is 12-18 mmol / L, then ultrasonic power is 170-240 W, ultrasonic temperature is 40-60°C, and reaction time is 30-90 min.After the reaction is completed, Amomum tsao-ko polysaccharide degradation solution is obtained, and Amomum tsao-ko degradation polysaccharides are prepared through dialysis, purification, drying and grinding processes.The application uses ultrasonic wave assisted combination H2O2-Vc free radical degradation technology to degrade Amomum tsao-ko polysaccharides.This method not only effectively reduces the molecular weight of Amomum tsao-ko polysaccharides, but also successfully retains the original monosaccharide type and biological activity of Amomum tsao-ko polysaccharides, and experiments prove that it also has significant neuroprotective effect, and can be developed into a drug with neuroprotective effect as an active ingredient.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

The application discloses a kind of fresh-keeping processing technology for reducing angelica polysaccharide loss, and relates to the technical field of traditional Chinese medicinal material origin processing, comprising the following steps: S1, material selection, S2, cleaning, S3, enzyme treatment, S4, cutting, S5, subsection drying.The application combines the technical means of time-limited water washing after harvesting, time-limited enzyme treatment after cleaning, fresh-cutting and gradient temperature subsection drying organically, and produces synergistic effect, wherein the enzyme treatment can effectively inhibit the activity of polysaccharide degradation enzyme in angelica fresh root, and block the enzymatic degradation pathway of polysaccharide from the source, and the gradient temperature subsection drying can significantly reduce the thermal degradation damage of polysaccharide at high temperature while ensuring the dehydration efficiency, and the comparative experiment of the embodiment shows that the polysaccharide retention rate of angelica decoction pieces processed by the application is significantly higher than that of traditional fresh-keeping processing technology and traditional dry-cutting technology, and the polysaccharide loss is effectively reduced.

The application discloses a method for inhibiting and killing water-bloom blue algae by using black soldier fly larvae manure, first, black soldier fly 3-5 instar larvae manure is selected and laid flat in a cool and ventilated place to be naturally air-dried until the water content is below 12%, then the air-dried black soldier fly larvae manure is crushed to above 20 meshes, then the crushed black soldier fly larvae manure is uniformly spread to a water-bloom blue algae gathering area at a downwind opening at a dosage of 5-20 kg / (mu*m), finally, VC and VB 12 The application uses the high polysaccharide degradation performance of microorganisms in black soldier fly larvae manure to safely and effectively inhibit and kill water-bloom blue algae, and further reduce the harm of water-bloom blue algae in a pond to breeding production in a short time.

The present invention discloses a marine galacto-oligosaccharide lipid and its preparation method and application, which belongs to the field of marine biological resource utilization and drug development. The present invention combines the marine red algae polysaccharide degradation technology with the high efficiency of reductive amination fatty chain modification for the first time, and realizes the modification of saturated fatty chains of different lengths of marine galacto-oligosaccharides with different polymerization degrees. And through cell-level pharmacodynamic evaluation, it is proved that marine galacto-oligosaccharide lipid can significantly inhibit the release of nitric oxide and the expression of inflammatory factors such as interleukin-6 and tumor necrosis factor α in microglia, and has the activity of inhibiting neuroinflammatory; at the same time, it can significantly inhibit the proliferation of tumor cells U87MG and GL261, has certain anti-tumor potential, and can be used to prepare drugs for preventing and / or treating neuroinflammation and anti-tumor related diseases. Therefore, the marine galacto-oligosaccharide lipid prepared by the present invention has high clinical application value and development prospects.

The invention discloses a preparation method and application of sea cucumber polysaccharide, and belongs to the field of food and medicine. According to the method, the sea cucumber intestines are used as raw materials, only the sea cucumber intestines are simply washed in the pretreatment stage, on one hand, polysaccharide loss caused by excessive treatment is avoided, on the other hand, a natural grinding medium is provided for subsequent use of bone paste grinding homogenate, and tissue breaking is facilitated; and then the sand grains and the tissue homogenate are separated through standing. On the basis, polysaccharide extraction is performed by fully utilizing the autolysis characteristic of sea cucumber intestines, and a NaCl solution with a specific concentration is key added in the autolysis stage to inhibit polysaccharide degradation, so that the yield of total sugar and sulfated polysaccharide is increased. Furthermore, a synergistic treatment process of alkaline hydrolysis and enzymolysis is adopted, so that polysaccharide components in the sea cucumber intestines are fully released. Experiments show that the obtained polysaccharide can effectively relieve immunosuppression and intestinal barrier injury caused by cyclophosphamide, and the whole immune function of the body is recovered by adjusting intestinal flora and metabolites thereof.

The invention discloses an extraction method of polygonatum polysaccharide, and belongs to the technical field of natural product extraction. The method comprises the steps of raw material pretreatment, squeezing extraction, standing centrifugation, macroporous resin adsorption separation, nanofiltration membrane grading purification, Sevage deproteinization, neutral alumina decoloration, freeze drying and the like. The method is mild in process condition and simple and convenient to operate, polysaccharide degradation can be effectively reduced through squeezing and low-temperature separation technologies, and the purity and yield of the polysaccharide are remarkably improved by combining resin separation and nanofiltration membrane technologies. Through detection, the purity of the obtained polysaccharide can reach 90% or above, the protein content is lower than 1%, and the method is suitable for polysaccharide preparation of plant rhizome raw materials and the fields of medicines, food or health care products.

Provided is a water-in-oil emulsion composition that includes fat, water, and an emulsifier and can enhance the flavor and body of a food product and, as necessary, give the food product succulence. The emulsifier includes an emulsifier A and an emulsifier B and / or an emulsifier C. The emulsifier A is at least one type of emulsifier selected from the group that consists of polyglycerin condensed ricinoleic acid esters, glycerin fatty acid esters, sucrose fatty acid esters, and lecithin and has an HLB of no more than 9.0. The emulsifier B is a fatty acid ester and includes a saturated fatty acid as a constituent fatty acid. The emulsifier C is a fatty acid ester and includes only unsaturated fatty acids as constituent fatty acids. The aqueous phase contains a polysaccharide degradation product, an alkali metal element, and magnesium.

The invention belongs to the technical field of biochemistry, and particularly discloses an algal polysaccharide degradation method, a degradation product and application of the degradation product. According to the method provided by the invention, sodium persulfate (Na2S2O8) is taken as an oxidizing agent, and the low-molecular-weight active polysaccharide is prepared by efficiently cracking a polysaccharide chain through a free radical chain reaction. Compared with a traditional chemical degradation method, a physical degradation method or an enzymatic degradation method, the method has the advantages that the degradation efficiency is obviously improved, the uniformity of degradation products is high, the process compatibility is strong, and the cost is low. Polysaccharide degradation products with different molecular weights can be obtained by adjusting the concentration of sodium persulfate, the degradation temperature and the degradation time, and an efficient and controllable scheme is provided for industrial production of low-molecular-weight active polysaccharides. The obtained algal polysaccharide degradation product can be used for preparing drugs for improving intestinal microecological balance and relieving and / or treating colitis, can repair intestinal mucosa structures, promote inflammatory cell regression, reduce proinflammatory factor expression and the like, and has potential clinical application potential.

An ultrasonic tremella polysaccharide extraction process temperature control and flow rate coordinated control system of the present invention belongs to the technical field of automatic control, and comprises: an oxidation potential sensing module for online detecting a tremella raw material hydration suspension of a tremella raw material to generate a tremella raw material oxidation potential index for quantifying an oxidative stress level; the dynamic parameter prediction module is used for receiving the oxidation potential index of the tremella fuciformis raw material and calculating an optimal temperature set value and an optimal flow rate set value based on a preset polysaccharide degradation risk model and an extraction efficiency model; the coordination control execution module is used for receiving the optimal temperature set value and the optimal flow speed set value and driving the heating system and the fluid conveying system to track the set values, and the problem that in the prior art, production is unstable due to the fact that individualized differences of raw materials cannot be sensed is solved.

The application belongs to the technical field of water treatment, and particularly relates to a membrane pollution control method using polysaccharide-degrading bacteria. The application takes activated sludge in a MBR as a source of screening bacteria, and through culture with sodium alginate as a substrate, a strain of bacteria with polysaccharide-degrading performance, i.e., P.carboxydivorans P-1 strain, is screened and separated. The bacterial solution of the strain is injected and encapsulated in a hollow fiber membrane cavity, and the hollow fiber membrane encapsulating the polysaccharide-degrading bacteria is placed in a membrane bioreactor, which can be used for membrane pollution control. The above technical scheme of the application can effectively reduce the content of polysaccharide in soluble microbial products (SMP) and extracellular polymeric substance (EPS) in the membrane bioreactor, reduce the viscosity of the activated sludge, thereby reducing the adhesion of microorganisms on the membrane surface, and durably and effectively controlling the membrane pollution of the membrane bioreactor (MBR).

This invention provides an agar oligosaccharide with antioxidant function, its preparation method, and its application, belonging to the field of natural polysaccharide degradation technology. This invention utilizes H2O2 / Fe... 2+ The system generates hydroxyl radicals to degrade agar polysaccharides, utilizing H2O2 in Fe... 2+ Catalysis oxidizes and breaks the glycosidic bonds in agar polysaccharides, degrading them into low-molecular-weight fragments to obtain agar oligosaccharides with good antioxidant activity. These fragments are then combined with vitamin C, which reduces the color of the lyophilized and reconstituted agar oligosaccharide solution and imparts stronger antioxidant activity to the degraded agar oligosaccharides. The preparation method provided by this invention has a simple process flow, low equipment requirements, low cost, and a high yield of agar oligosaccharides, reaching over 85%, making it suitable for large-scale industrial application.

The invention belongs to the technical field of water treatment, and particularly relates to a membrane pollution control method applying polysaccharide degrading bacteria. Activated sludge in an MBR is used as a bacterium screening source, sodium alginate is used as a matrix for culture, a bacterium with polysaccharide degradation performance, namely a P.carboxydivorans P-1 strain, is screened and separated from the bacterium screening source, a bacterium solution of the strain is injected and packaged in a hollow fiber membrane cavity, then the hollow fiber membrane packaging the polysaccharide degradation bacterium is placed in a membrane bioreactor, and the membrane bioreactor is used for preparing the polysaccharide degradation bacterium. The method can be used for membrane pollution control. By adopting the technical scheme, the content of polysaccharide in soluble microbial products (SMP) and extracellular polymeric substances (EPS) in the membrane bioreactor can be effectively reduced, and the viscosity of activated sludge is reduced, so that the adhesion of microorganisms on the surface of a membrane is reduced, and the membrane pollution of the membrane bioreactor (MBR) is permanently and effectively controlled.

The invention discloses a preparation method of selenium-enriched Chinese herbal medicine pills for consolidating basis, which belongs to the technical field of nutrient enrichment and is realized by five steps of biological enhancement planting, compound enzyme hydrolysis, two-stage fermentation, calcination reaction and finished product preparation. According to the preparation method of the selenium-rich traditional Chinese herbal medicine pill for consolidating basis and cultivating primordial qi, the selenium content in prepared polygonum multiflorum, astragalus membranaceus and nutgrass flatsedge is increased in a selenium biological enhancement planting mode, and then protein and polysaccharide in enzymolysis products are degraded into small molecule peptides and oligosaccharides through compound enzyme hydrolysis and two-time fermentation, so that the selenium-rich traditional Chinese herbal medicine pill for consolidating basis and cultivating primordial qi can be easily absorbed by a human body. And finally, by calcining the sea clam shells and mixing the sea clam shells with the fermentation liquor, the utilization rate of selenium can be further increased. The whole process is simple and easy to implement, convenient to operate, low in production cost and suitable for industrial large-scale production.

The invention discloses a small protein PoxTMA7, which has an amino acid sequence as shown in SEQ. ID. NO.1. The invention also discloses a preparation method of the small protein PoxTMA7. In addition, an encoding gene of the small protein PoxTMA7 is also obtained, and the encoding gene has a base sequence of SEQ. ID. Researches show that the PoxTMA7 plays an important role in the translation stage of biosynthesis of the Penicillium oxalicum plant polysaccharide degrading enzyme, and under the condition of induced culture of crystalline cellulose, the PoxTMA7 influences the production of cellulase and xylanase by regulating the translation efficiency of key cellulase and xylanase genes cbh1, cbh2, eg1 and xyn11A. Therefore, the small protein PoxTMA7 or the coding gene thereof has application potential in influencing the generation of plant polysaccharide degrading enzymes or regulating the translation of related genes, and can be used for regulating the yield of cellulase and xylanase of microorganisms. In addition, identification and excavation of the regulatory factor for the translation efficiency of the Penicillium oxalicum plant polysaccharide degrading enzyme have theoretical guiding significance for constructing an engineering strain for high-yield plant polysaccharide degrading enzyme.

The application belongs to the technical field of food deep processing, and particularly relates to a red date degraded polysaccharide, a degraded polysaccharide component, and a preparation method and application thereof. The application provides a preparation method of the red date degraded polysaccharide, in which the red date polysaccharide is degraded by using an ultrasonic wave assisted method combined with H2O2-Vc, and the red date degraded polysaccharide is separated and purified by using a DEAE-Sepharose Fast Flow gel column and a Sephacryl S-100 chromatographic column. The red date degraded polysaccharide and the polysaccharide component have the following characteristics: after the degradation treatment, the fragmentation degree of the polysaccharide surface is deepened, the degradation does not change the basic structure of the polysaccharide, and the apparent morphological characteristics change to a certain extent; the degradation treatment can improve the antioxidant capacity of the polysaccharide and is inversely proportional to the polysaccharide molecular weight.

The invention relates to the use of at least one amino acid analogue or a protein as a zootechnical additive for the feed of farmed insects, said amino acid analogue being selected from 2-hydroxy-4-methylthiobutanoic acid and 2-hydroxy-4-methylselenobutanoic acid and the protein being selected from phytases and non-starch polysaccharide degradation enzymes (NSP-ases); a feed for farmed insects; a method for supplementing the feed of farmed insects and a method for increasing the performance of an insect farm.

The invention relates to the technical field of traditional Chinese medicine extracts, in particular to a preparation process of a veterinary radix bupleuri extracting solution composition. Comprising the following steps: extracting mulberry leaf heteropolysaccharide; preparing a saikosaponin extract; preparing volatile oil; and preparing the veterinary radix bupleuri extracting solution composition. The mulberry leaf polysaccharide is added into the radix bupleuri extraction process, the mulberry leaf polysaccharide contains a large amount of hydroxyl and carboxyl, and in the water extraction process, cell walls of radix bupleuri are softened through hydrogen bonds and electrostatic interaction, a cellulose-hemicellulose network is destroyed, and release of vinegar radix bupleuri polysaccharide is accelerated. Meanwhile, the viscosity of an extraction system can be increased and polysaccharide degradation can be reduced due to the hydrophilic colloid characteristic, then, vinegar-processed bupleurum polysaccharide and mulberry leaf polysaccharide form mixed micelles in an alkaline ethanol environment, a hydrophobic core can wrap saikosaponin molecules, the solubility of saponin in an ethanol-water system is remarkably improved, and therefore the extraction rate of saikosaponin is increased.

The invention relates to the technical field of coating agent preparation, in particular to a bionic seed coating agent and an intelligent preparation method. The preparation method comprises the following steps: adding nano-montmorillonite into a mixture of artemisia desertorum seed zymolyte subjected to bubble removal and xanthan gum; sequentially adding auxin microcapsules and sea-buckthorn polysaccharide degradation products into the mixture of the artemisia desertorum seed zymolyte and xanthan gum after the bubbles are removed, and mixing insecticide microcapsules with a sodium alginate solution; the spraying distance is adjusted, or the opening and closing frequency before spraying is adjusted; spraying the growth regulation layer coating agent on the outer surfaces of the seeds, and performing weak ultraviolet irradiation; spraying the isolating layer coating agent, drying the isolating layer coating agent according to a mode of first high-temperature hot air and then low-temperature cold air, and adjusting the air speed at the starting moment of the high-temperature hot air according to the volatilization rate of the first bionic seed coating layer; and spraying a water sticking layer coating agent, and drying with hot air to form the seed coating coated with the finished bionic seed coating agent. According to the invention, the landing stability of the seeds in the desert region is improved.

The invention relates to the technical field of thermostable enzymes, in particular to beta-1, 3-glucosidase and a method for improving the enzyme activity of the beta-1, 3-glucosidase. The beta-glucosidase encoding gene YALI1B03564g is identified in a yarrowia lipolytica genome for the first time and is named as beta-1, 3-glucosidase, and the beta-glucosidase encoding gene YALI1B03564g is confirmed to have unique beta-1, 3-glucosidic bond substrate specificity through heterologous expression and enzymatic characterization; the thermal stability of the enzyme is further remarkably improved through rational design, and the enzyme is applied to in-vitro polysaccharide degradation; the technical scheme provided by the invention further lays a foundation for large-scale production of the functional yeast beta-glucan oligosaccharide.

The invention discloses a morchella polysaccharide extraction and purification device and process, and relates to the technical field of polysaccharide preparation, the morchella polysaccharide extraction and purification device comprises a supporting seat, the interior of the supporting seat is fixedly connected with a supporting plate, and the upper part of the supporting seat is provided with a pretreatment mechanism for crushing a morchella raw material; an extraction mechanism used for extracting morchella polysaccharide is arranged on the top surface of the supporting plate, a purification tank used for achieving triple filtration is fixedly connected to the interior of the supporting seat, and a liquid discharging pipe used for extracting filtered supernate penetrates through and is fixedly connected to and communicates with the bottom surface of the purification tank; a dialysis box fixedly connected with the bottom face of the supporting base is fixedly connected and communicated with the side, away from the purification tank, of the liquid discharging pipe, reciprocating rolling extrusion is conducted in the roller crushing box, so that a three-dimensional grinding path is formed, and during stirring, the reciprocating lead screw rotates to drive the material pushing cylinder to reciprocate in the rollers, so that the grinding efficiency is improved, and the grinding efficiency is improved. Therefore, a small amount of dry ice is fed into the roller for multiple times, the crushing environment temperature is stabilized below-10 DEG C, and polysaccharide degradation is avoided.