28 results about "Polysaccharide degradation" patented technology

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

A method for preparing a roasted food product, suitable for preparing a coffee-like 5 beverage product, starting from one or more pulses, comprising one or more pretreatment steps followed by one or more roasting steps, wherein preferably the roasting temperature is in the range of 75 °C to 250 °C, more preferably in the range of 125 °C to 225 °C, even more preferably in the range of 150 °C to 200 °C, wherein the pretreatment comprises the following steps: ▪ at least one polysaccharide degradation step, preferably for starch degradation; ▪ at least one acidification step, preferably by fermentation, to obtain a pH preferably below 6 of the roasted food product, more preferably below 5.5, most preferably below 5; ▪ at least one drying step, wherein the water content of the pulses obtained by the polysaccharide degradation and acidification steps is adjusted to preferably 14 weight% or less, more preferably to 12 weight%, most preferably to 10 weight% as compared to the total weight of the pretreated pulses.

The application discloses a tremella aurantialba polysaccharide degradation product, which is prepared by degrading tremella aurantialba polysaccharide by a method of combining H2O2 and vitamin C and selecting degradation products with a molecular weight greater than 100 k Da and a molecular weight of 1 k Da to 100 k Da. Effective degradation of the tremella aurantialba polysaccharide can greatly improve the bioavailability of the tremella aurantialba polysaccharide, and the tremella aurantialba polysaccharide degradation product I (E-TAP I) and the tremella aurantialba polysaccharide degradation product II (E-TAP II) have good application prospects in skin thermal injury repair.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.

The application provides a biological skin activity fermentation liquor with multiple effects, a preparation method thereof and a cosmetic, and relates to the cosmetic field.The method comprises the following steps: mixing camellia oleifera seed meal, protease, cellulase, lipase and water to obtain a first mixture; performing enzymolysis on the first mixture to obtain a fermentation substrate; performing second mixing on the fermentation substrate, glucose, peptone, yeast powder, disodium hydrogen phosphate and citric acid, and sterilizing to obtain a fermentation culture medium; activating bacillus velezensis through an LB culture medium to obtain a seed liquid; performing third mixing on the seed liquid and the fermentation culture medium, and performing fermentation to obtain a camellia oleifera seed meal fermentation liquor; performing centrifugation on the camellia oleifera seed meal fermentation liquor to obtain a centrifuged fermentation liquor; performing fourth mixing on the centrifuged fermentation liquor and polysaccharide-degrading enzyme, performing enzymolysis, and performing filtration to obtain the biological skin activity fermentation liquor with multiple effects.The method not only improves the high-value utilization of the camellia oleifera seed meal, but also obtains the biological skin activity fermentation liquor with high safety and outstanding activity.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

The application discloses a kind of fresh-keeping processing technology for reducing angelica polysaccharide loss, and relates to the technical field of traditional Chinese medicinal material origin processing, comprising the following steps: S1, material selection, S2, cleaning, S3, enzyme treatment, S4, cutting, S5, subsection drying.The application combines the technical means of time-limited water washing after harvesting, time-limited enzyme treatment after cleaning, fresh-cutting and gradient temperature subsection drying organically, and produces synergistic effect, wherein the enzyme treatment can effectively inhibit the activity of polysaccharide degradation enzyme in angelica fresh root, and block the enzymatic degradation pathway of polysaccharide from the source, and the gradient temperature subsection drying can significantly reduce the thermal degradation damage of polysaccharide at high temperature while ensuring the dehydration efficiency, and the comparative experiment of the embodiment shows that the polysaccharide retention rate of angelica decoction pieces processed by the application is significantly higher than that of traditional fresh-keeping processing technology and traditional dry-cutting technology, and the polysaccharide loss is effectively reduced.

The application discloses a method for inhibiting and killing water-bloom blue algae by using black soldier fly larvae manure, first, black soldier fly 3-5 instar larvae manure is selected and laid flat in a cool and ventilated place to be naturally air-dried until the water content is below 12%, then the air-dried black soldier fly larvae manure is crushed to above 20 meshes, then the crushed black soldier fly larvae manure is uniformly spread to a water-bloom blue algae gathering area at a downwind opening at a dosage of 5-20 kg / (mu*m), finally, VC and VB 12 The application uses the high polysaccharide degradation performance of microorganisms in black soldier fly larvae manure to safely and effectively inhibit and kill water-bloom blue algae, and further reduce the harm of water-bloom blue algae in a pond to breeding production in a short time.

The invention discloses a preparation method and application of sea cucumber polysaccharide, and belongs to the field of food and medicine. According to the method, the sea cucumber intestines are used as raw materials, only the sea cucumber intestines are simply washed in the pretreatment stage, on one hand, polysaccharide loss caused by excessive treatment is avoided, on the other hand, a natural grinding medium is provided for subsequent use of bone paste grinding homogenate, and tissue breaking is facilitated; and then the sand grains and the tissue homogenate are separated through standing. On the basis, polysaccharide extraction is performed by fully utilizing the autolysis characteristic of sea cucumber intestines, and a NaCl solution with a specific concentration is key added in the autolysis stage to inhibit polysaccharide degradation, so that the yield of total sugar and sulfated polysaccharide is increased. Furthermore, a synergistic treatment process of alkaline hydrolysis and enzymolysis is adopted, so that polysaccharide components in the sea cucumber intestines are fully released. Experiments show that the obtained polysaccharide can effectively relieve immunosuppression and intestinal barrier injury caused by cyclophosphamide, and the whole immune function of the body is recovered by adjusting intestinal flora and metabolites thereof.

The invention discloses an extraction method of polygonatum polysaccharide, and belongs to the technical field of natural product extraction. The method comprises the steps of raw material pretreatment, squeezing extraction, standing centrifugation, macroporous resin adsorption separation, nanofiltration membrane grading purification, Sevage deproteinization, neutral alumina decoloration, freeze drying and the like. The method is mild in process condition and simple and convenient to operate, polysaccharide degradation can be effectively reduced through squeezing and low-temperature separation technologies, and the purity and yield of the polysaccharide are remarkably improved by combining resin separation and nanofiltration membrane technologies. Through detection, the purity of the obtained polysaccharide can reach 90% or above, the protein content is lower than 1%, and the method is suitable for polysaccharide preparation of plant rhizome raw materials and the fields of medicines, food or health care products.

Provided is a water-in-oil emulsion composition that includes fat, water, and an emulsifier and can enhance the flavor and body of a food product and, as necessary, give the food product succulence. The emulsifier includes an emulsifier A and an emulsifier B and / or an emulsifier C. The emulsifier A is at least one type of emulsifier selected from the group that consists of polyglycerin condensed ricinoleic acid esters, glycerin fatty acid esters, sucrose fatty acid esters, and lecithin and has an HLB of no more than 9.0. The emulsifier B is a fatty acid ester and includes a saturated fatty acid as a constituent fatty acid. The emulsifier C is a fatty acid ester and includes only unsaturated fatty acids as constituent fatty acids. The aqueous phase contains a polysaccharide degradation product, an alkali metal element, and magnesium.

The invention belongs to the technical field of biochemistry, and particularly discloses an algal polysaccharide degradation method, a degradation product and application of the degradation product. According to the method provided by the invention, sodium persulfate (Na2S2O8) is taken as an oxidizing agent, and the low-molecular-weight active polysaccharide is prepared by efficiently cracking a polysaccharide chain through a free radical chain reaction. Compared with a traditional chemical degradation method, a physical degradation method or an enzymatic degradation method, the method has the advantages that the degradation efficiency is obviously improved, the uniformity of degradation products is high, the process compatibility is strong, and the cost is low. Polysaccharide degradation products with different molecular weights can be obtained by adjusting the concentration of sodium persulfate, the degradation temperature and the degradation time, and an efficient and controllable scheme is provided for industrial production of low-molecular-weight active polysaccharides. The obtained algal polysaccharide degradation product can be used for preparing drugs for improving intestinal microecological balance and relieving and / or treating colitis, can repair intestinal mucosa structures, promote inflammatory cell regression, reduce proinflammatory factor expression and the like, and has potential clinical application potential.

An ultrasonic tremella polysaccharide extraction process temperature control and flow rate coordinated control system of the present invention belongs to the technical field of automatic control, and comprises: an oxidation potential sensing module for online detecting a tremella raw material hydration suspension of a tremella raw material to generate a tremella raw material oxidation potential index for quantifying an oxidative stress level; the dynamic parameter prediction module is used for receiving the oxidation potential index of the tremella fuciformis raw material and calculating an optimal temperature set value and an optimal flow rate set value based on a preset polysaccharide degradation risk model and an extraction efficiency model; the coordination control execution module is used for receiving the optimal temperature set value and the optimal flow speed set value and driving the heating system and the fluid conveying system to track the set values, and the problem that in the prior art, production is unstable due to the fact that individualized differences of raw materials cannot be sensed is solved.

The application belongs to the technical field of water treatment, and particularly relates to a membrane pollution control method using polysaccharide-degrading bacteria. The application takes activated sludge in a MBR as a source of screening bacteria, and through culture with sodium alginate as a substrate, a strain of bacteria with polysaccharide-degrading performance, i.e., P.carboxydivorans P-1 strain, is screened and separated. The bacterial solution of the strain is injected and encapsulated in a hollow fiber membrane cavity, and the hollow fiber membrane encapsulating the polysaccharide-degrading bacteria is placed in a membrane bioreactor, which can be used for membrane pollution control. The above technical scheme of the application can effectively reduce the content of polysaccharide in soluble microbial products (SMP) and extracellular polymeric substance (EPS) in the membrane bioreactor, reduce the viscosity of the activated sludge, thereby reducing the adhesion of microorganisms on the membrane surface, and durably and effectively controlling the membrane pollution of the membrane bioreactor (MBR).

This invention provides an agar oligosaccharide with antioxidant function, its preparation method, and its application, belonging to the field of natural polysaccharide degradation technology. This invention utilizes H2O2 / Fe... 2+ The system generates hydroxyl radicals to degrade agar polysaccharides, utilizing H2O2 in Fe... 2+ Catalysis oxidizes and breaks the glycosidic bonds in agar polysaccharides, degrading them into low-molecular-weight fragments to obtain agar oligosaccharides with good antioxidant activity. These fragments are then combined with vitamin C, which reduces the color of the lyophilized and reconstituted agar oligosaccharide solution and imparts stronger antioxidant activity to the degraded agar oligosaccharides. The preparation method provided by this invention has a simple process flow, low equipment requirements, low cost, and a high yield of agar oligosaccharides, reaching over 85%, making it suitable for large-scale industrial application.

The invention belongs to the technical field of water treatment, and particularly relates to a membrane pollution control method applying polysaccharide degrading bacteria. Activated sludge in an MBR is used as a bacterium screening source, sodium alginate is used as a matrix for culture, a bacterium with polysaccharide degradation performance, namely a P.carboxydivorans P-1 strain, is screened and separated from the bacterium screening source, a bacterium solution of the strain is injected and packaged in a hollow fiber membrane cavity, then the hollow fiber membrane packaging the polysaccharide degradation bacterium is placed in a membrane bioreactor, and the membrane bioreactor is used for preparing the polysaccharide degradation bacterium. The method can be used for membrane pollution control. By adopting the technical scheme, the content of polysaccharide in soluble microbial products (SMP) and extracellular polymeric substances (EPS) in the membrane bioreactor can be effectively reduced, and the viscosity of activated sludge is reduced, so that the adhesion of microorganisms on the surface of a membrane is reduced, and the membrane pollution of the membrane bioreactor (MBR) is permanently and effectively controlled.

The application belongs to the technical field of food deep processing, and particularly relates to a red date degraded polysaccharide, a degraded polysaccharide component, and a preparation method and application thereof. The application provides a preparation method of the red date degraded polysaccharide, in which the red date polysaccharide is degraded by using an ultrasonic wave assisted method combined with H2O2-Vc, and the red date degraded polysaccharide is separated and purified by using a DEAE-Sepharose Fast Flow gel column and a Sephacryl S-100 chromatographic column. The red date degraded polysaccharide and the polysaccharide component have the following characteristics: after the degradation treatment, the fragmentation degree of the polysaccharide surface is deepened, the degradation does not change the basic structure of the polysaccharide, and the apparent morphological characteristics change to a certain extent; the degradation treatment can improve the antioxidant capacity of the polysaccharide and is inversely proportional to the polysaccharide molecular weight.

The invention relates to the use of at least one amino acid analogue or a protein as a zootechnical additive for the feed of farmed insects, said amino acid analogue being selected from 2-hydroxy-4-methylthiobutanoic acid and 2-hydroxy-4-methylselenobutanoic acid and the protein being selected from phytases and non-starch polysaccharide degradation enzymes (NSP-ases); a feed for farmed insects; a method for supplementing the feed of farmed insects and a method for increasing the performance of an insect farm.

The invention relates to the technical field of traditional Chinese medicine extracts, in particular to a preparation process of a veterinary radix bupleuri extracting solution composition. Comprising the following steps: extracting mulberry leaf heteropolysaccharide; preparing a saikosaponin extract; preparing volatile oil; and preparing the veterinary radix bupleuri extracting solution composition. The mulberry leaf polysaccharide is added into the radix bupleuri extraction process, the mulberry leaf polysaccharide contains a large amount of hydroxyl and carboxyl, and in the water extraction process, cell walls of radix bupleuri are softened through hydrogen bonds and electrostatic interaction, a cellulose-hemicellulose network is destroyed, and release of vinegar radix bupleuri polysaccharide is accelerated. Meanwhile, the viscosity of an extraction system can be increased and polysaccharide degradation can be reduced due to the hydrophilic colloid characteristic, then, vinegar-processed bupleurum polysaccharide and mulberry leaf polysaccharide form mixed micelles in an alkaline ethanol environment, a hydrophobic core can wrap saikosaponin molecules, the solubility of saponin in an ethanol-water system is remarkably improved, and therefore the extraction rate of saikosaponin is increased.

The invention relates to the technical field of coating agent preparation, in particular to a bionic seed coating agent and an intelligent preparation method. The preparation method comprises the following steps: adding nano-montmorillonite into a mixture of artemisia desertorum seed zymolyte subjected to bubble removal and xanthan gum; sequentially adding auxin microcapsules and sea-buckthorn polysaccharide degradation products into the mixture of the artemisia desertorum seed zymolyte and xanthan gum after the bubbles are removed, and mixing insecticide microcapsules with a sodium alginate solution; the spraying distance is adjusted, or the opening and closing frequency before spraying is adjusted; spraying the growth regulation layer coating agent on the outer surfaces of the seeds, and performing weak ultraviolet irradiation; spraying the isolating layer coating agent, drying the isolating layer coating agent according to a mode of first high-temperature hot air and then low-temperature cold air, and adjusting the air speed at the starting moment of the high-temperature hot air according to the volatilization rate of the first bionic seed coating layer; and spraying a water sticking layer coating agent, and drying with hot air to form the seed coating coated with the finished bionic seed coating agent. According to the invention, the landing stability of the seeds in the desert region is improved.

The invention relates to the technical field of thermostable enzymes, in particular to beta-1, 3-glucosidase and a method for improving the enzyme activity of the beta-1, 3-glucosidase. The beta-glucosidase encoding gene YALI1B03564g is identified in a yarrowia lipolytica genome for the first time and is named as beta-1, 3-glucosidase, and the beta-glucosidase encoding gene YALI1B03564g is confirmed to have unique beta-1, 3-glucosidic bond substrate specificity through heterologous expression and enzymatic characterization; the thermal stability of the enzyme is further remarkably improved through rational design, and the enzyme is applied to in-vitro polysaccharide degradation; the technical scheme provided by the invention further lays a foundation for large-scale production of the functional yeast beta-glucan oligosaccharide.

The invention discloses a morchella polysaccharide extraction and purification device and process, and relates to the technical field of polysaccharide preparation, the morchella polysaccharide extraction and purification device comprises a supporting seat, the interior of the supporting seat is fixedly connected with a supporting plate, and the upper part of the supporting seat is provided with a pretreatment mechanism for crushing a morchella raw material; an extraction mechanism used for extracting morchella polysaccharide is arranged on the top surface of the supporting plate, a purification tank used for achieving triple filtration is fixedly connected to the interior of the supporting seat, and a liquid discharging pipe used for extracting filtered supernate penetrates through and is fixedly connected to and communicates with the bottom surface of the purification tank; a dialysis box fixedly connected with the bottom face of the supporting base is fixedly connected and communicated with the side, away from the purification tank, of the liquid discharging pipe, reciprocating rolling extrusion is conducted in the roller crushing box, so that a three-dimensional grinding path is formed, and during stirring, the reciprocating lead screw rotates to drive the material pushing cylinder to reciprocate in the rollers, so that the grinding efficiency is improved, and the grinding efficiency is improved. Therefore, a small amount of dry ice is fed into the roller for multiple times, the crushing environment temperature is stabilized below-10 DEG C, and polysaccharide degradation is avoided.

The present application relates to the technical field of oligosaccharide, in particular to a Dioscorea panthaica Prain et Buck oligosaccharide, a preparation method thereof and application of the Dioscorea panthaica Prain et Buck oligosaccharide in preparing a product for preventing or treating ulcerative colitis. In the present application, Dioscorea panthaica Prain et Buck polysaccharide and a ferrous ion solution are mixed, pH is adjusted to 2.0-6.0, a hydrogen peroxide solution is added, and ultrasonic treatment is carried out in an ultrasonic cell crusher to obtain a Dioscorea panthaica Prain et Buck polysaccharide degradation solution; the Dioscorea panthaica Prain et Buck polysaccharide degradation solution and a sodium hydroxide solution are mixed, solid substances and hydrogen peroxide are removed, and after purification and drying, the Dioscorea panthaica Prain et Buck oligosaccharide is obtained. The Dioscorea panthaica Prain et Buck oligosaccharide can be used for preparing a product related to preventing or treating ulcerative colitis.

The invention discloses application of a mutant of a 5'untranslated region (5 '-UTR) of an endo-beta-1, 4-glucanase gene eg1 or an upstream open reading frame (uORF) of the endo-beta-1, 4-glucanase gene eg1 or the upstream open reading frame (uORF) of the endo-beta-1, 4-glucanase gene eg1 to regulation and control of the expression of a fungal cellulase gene eg1 or regulation and control of the yield of cellulase. Researches show that the uORF1, the uORF3 and the uORF7 have a core regulation effect, the uORF1 and the uORF3 have a negative regulation effect in the biosynthesis process of the endo-beta-1, 4-glucanase of the penicillium oxalicum, and the yield of the cellulase can be remarkably increased by the mutation of the initiation codon of the endo-beta-1, 4-glucanase of the penicillium oxalicum; and the uORF7 plays a positive regulation role. Therefore, the uORF and the mutants thereof have huge application potential in precise regulation and control of plant polysaccharide degrading enzyme production or related gene expression, and can be used for constructing genetic engineering strains for high-yield cellulase; the identification and control of the uORF regulatory element of the penicillium oxalicum endo-beta-1, 4-glucanase gene disclosed by the invention have important theoretical guidance and practical values for increasing the yield of industrial enzyme preparations through a molecular breeding means.

The application discloses a method for improving gene transformation efficiency of a microorganism polysaccharide-producing strain, and the method comprises the following steps: treating the microorganism polysaccharide-producing strain by using a polysaccharide-degrading enzyme, removing the wrapping of the polysaccharide on the strain cell, obtaining the cell, and preparing a competent cell; then, an exogenous plasmid is transformed into the treated competent cell by using an electric shock; wherein the polysaccharide-degrading enzyme can degrade the extracellular polysaccharide produced in the culture process of the microorganism polysaccharide-producing strain. The application effectively improves the success rate and the transformation efficiency of plasmid transformation.

The invention discloses an endophytic growth-promoting bacterium NL-C4 and application thereof, and relates to the technical field of microorganisms. The endophytic growth-promoting bacterium NL-C4 disclosed by the invention is classified and named as Pantoea endophytic tiaa NL-C4, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO.M20251799, and the preservation date is August 8, 2024. The strain is excellent in saline-alkaline tolerance, can survive under the NaCl concentration of 8%, can grow in the pH range of 7-10, and has multiple functions of dissolving phosphorus, fixing nitrogen, producing IAA, siderophores and exopolysaccharides, degrading lignin and starch and the like. Pot experiments show that inoculation of the strain can significantly promote growth of sesbania in saline-alkali soil, increase indexes such as root length, biomass and the like, reduce pH, conductivity and total salt content of soil and have important application value in the fields of saline-alkali soil improvement and plant growth promotion.

This invention discloses a Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks and its preparation method, relating to the field of functional livestock and poultry feed technology. The feed includes: a basal feed and host-response triggered functional particles; the host-response triggered functional particles include a composite active core and a multi-factor coupling response layer coating the outside of the composite active core; the composite active core includes active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder; the active components derived from Poria cocos are Poria cocos polysaccharides and / or Poria cocos polysaccharide degradation fragments; the active components derived from Ganoderma lucidum spore powder are broken-cell wall Ganoderma lucidum spore powder, Ganoderma lucidum spore powder extract, and / or Ganoderma lucidum spore powder conversion products. This invention improves the accuracy of the timing of activity presentation and the specificity of the action site, promotes the effective contact, recognition, and utilization of active components in the intestinal tract, and helps maintain the stability and controllability of functional components.