Preparation method of rhizoma polygonati active peptide with homology of medicine and food

Abstract

The invention provides a preparation method, relates to the technical field of deep processing of rhizoma polygonati, and particularly discloses a preparation method of rhizoma polygonati active peptide with homology of medicine and food. The preparation method comprises the following steps: slicing, preparing water, cooking, degrading polysaccharide, carrying out targeted enzyme digestion, carrying out solid-liquid separation, deactivating enzyme, adding monomethoxy polyethylene glycol, carrying out combined modification, carrying out centrifugal purification, carrying out nanofiltration to remove polyethylene glycol, concentrating, and carrying out spray drying on the obtained concentrated solution to obtain a medicinal and edible polygonatum kingianum active peptide product. The preparation method is short in production period, low in cost and free of environmental pollution, and the obtained product is high in safety, high in purity and the like and can be widely applied to the aspects of food, health care products, special medical food, medicine and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of deep processing of polygonatum sibiricum, and in particular to a method for preparing a polygonatum sibiricum active peptide that is both medicinal and edible. Specifically, the present invention relates to a method for producing the polygonatum sibiricum active peptide that is both medicinal and edible. The method uses polygonatum sibiricum as a raw material and utilizes a biotransformation method to obtain the polygonatum sibiricum active peptide. Background Art

[0002] Polygonatum sibiricum is a perennial herbaceous plant of the genus Polygonatum in the Liliaceae family, including Polygonatum sibiricum, Polygonatum multiflorum, and Polygonatum yunnanensis. Polygonatum sibiricum has the effects of nourishing the kidneys and replenishing essence, nourishing yin and moistening the lungs, relieving fullness and replenishing qi, and promoting the production of body fluids and tonifying the spleen. Polygonatum sibiricum is mainly used to treat dry coughs due to lung dryness, physical weakness, and dry mouth due to long-term illness. It has good effects in antibacterial, stress-relieving, anti-fatigue, anti-aging, and treating cardiovascular diseases, tuberculosis, and chronic hepatitis. Polygonatum sibiricum has a sweet and neutral taste and enters the spleen, lung, and kidney meridians. As a traditional Chinese medicine with both medicinal and edible properties, it contains multiple active ingredients such as polysaccharides, steroidal saponins, and anthraquinones, and has significant therapeutic effects in metabolic diseases and immune regulation. In modern clinical practice, Polygonatum sibiricum is often used to treat diseases such as diabetes and arteriosclerosis. Dietary therapy is often used for Yin deficiency and lung dryness syndrome and cardiovascular diseases.

[0003] An academician of the Chinese Academy of Sciences noted, "Many conventional Chinese herbal extracts are actually prodrugs of their direct agents. Drug metabolism and transformation are ubiquitous in the body. The activity and toxicity of Chinese herbal medicines are often manifestations of their metabolites. The interactions between the various components in Chinese herbal formulas are all related to metabolism." New Chinese medicines, on the other hand, are based on the search for active ingredients within the metabolites of Chinese herbal ingredients. A professor at the Peking University School of Pharmacy pioneered the "human intestinal bacterial biotransformation study of Chinese herbal ingredients" in China in 1995, specifically tailored to the characteristics of Chinese herbal medicine. He established a systematic platform for Chinese herbal medicine absorption, distribution, metabolism, excretion, toxicity, and efficacy (ADMET / Act.) research, and addressed a key fundamental scientific issue in experimental medicine research: identifying effective and toxic chemical components of Chinese herbal medicines through intestinal bacterial biotransformation.

[0004] The lipid bilayer structure of biological membranes is a major barrier to drug entry into cells. Peptides are an ideal method for delivering substances to cells through these barriers. Small peptides can penetrate the lipid bilayer barrier of biological membranes and directly enter cells to exert regulatory effects. Small peptides also possess pharmacological activity, leading Swedish scientists to call them "cell-penetrating peptides." An academician of the Chinese Academy of Sciences noted, "When a malfunction occurs within a cell, macromolecules such as proteins and nucleic acids, which have the ability to repair damage, have difficulty entering the cell and reaching effective concentrations. Cell-penetrating peptides, however, can effectively transport other substances into the cell and possess inherent biological functions."

[0005] As a traditional Chinese medicine with both medicinal and edible properties, Polygonatum sibiricum possesses excellent pharmacological activity, and its comprehensive utilization should be actively explored. With economic development and improved living standards, awareness of disease prevention and health care is growing. In addition to its medicinal uses, Polygonatum sibiricum has been widely used in various health foods and functional foods. Although Polygonatum sibiricum extraction has a history of thousands of years, traditional processes, primarily based on separation and screening, suffer from shortcomings in extraction efficiency and energy consumption for active substances. For example, aqueous extraction and hydrophilic / lipophilic organic solvent extraction methods are used. Aqueous extraction yields low extraction rates for small non-polar peptides (such as hydrophobic active peptides). While organic solvents such as ethanol and chloroform can increase the yield of some fat-soluble components, they can easily denature and inactivate small peptides and carry the risk of solvent residues (e.g., methanol toxicity). Furthermore, in traditional processes, when Polygonatum sibiricum peptides coexist with macromolecular components such as polysaccharides and saponins, separation accuracy is insufficient, resulting in generally low levels of active peptides in the final product. Therefore, developing safer methods for producing Polygonatum sibiricum active peptides that are more easily absorbed and utilized by the body is of great significance.

[0006] In addition, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the applicant studied a large number of documents and patents when making the present invention, but due to space limitations, not all details and contents are listed in detail. However, this does not mean that the present invention does not have the characteristics of these prior arts. On the contrary, the present invention already has all the characteristics of the prior art, and the applicant reserves the right to add relevant prior art to the background technology. Summary of the Invention

[0007] The present invention aims to provide a method for preparing a polygonatum active peptide that is both medicinal and edible. The method utilizes enzyme degradation and metabolism, followed by modified synthesis and separation and purification, thereby changing the traditional solvent extraction method of traditional Chinese medicine. The resulting product achieves the goal of perfectly combining the active peptide with the active substance of traditional Chinese medicine. At the same time, the method reduces the antagonism of traditional Chinese medicine compatibility, making the product safer and highly feasible for large-scale industrial production.

[0008] In response to the problems of low active peptide retention rate, lack of metabolic synergy, insufficient bioavailability and unsustainable process in existing Polygonatum preparation technology, the present invention provides a method for preparing Polygonatum active peptide based on enzyme-directed degradation, targeted enzyme cleavage, combined modification, centrifugal purification, nanofiltration and concentration technology. The preparation method comprises the following steps:

[0009] (1) Raw material pretreatment: selecting a raw material of polygonatum, slicing it, adding purified water 10 to 12 times the weight of the raw material of polygonatum, stirring evenly, and steaming it at 90 to 95° C. for 3 to 3.5 hours to obtain the steamed raw material of polygonatum;

[0010] (2) Amylase hydrolysis: Cool the steamed Polygonatum sibiricum raw material to 65-67°C, add amylase, maintain the temperature at 65-67°C, and stir for 30-45 minutes to obtain a preliminary enzymatic hydrolysis product;

[0011] (3) Protease hydrolysis: Cool the product after preliminary hydrolysis to 50-55°C, add neutral protease, maintain the temperature at 50-55°C, and stir for 3.0-4.0 hours;

[0012] (4) Inactivation of enzyme: heating the hydrolyzed product to 90-95°C and inactivating the enzyme for 10-15 minutes;

[0013] (5) Primary separation: After inactivation of the enzyme, the filtrate is filtered using a decanter centrifuge and the filtrate is collected;

[0014] (6) Active peptide modification: The temperature of the separated filtrate was controlled at 35-37°C, and 0.1-0.2% of monomethoxy polyethylene glycol by weight of the raw material of Polygonatum was added, and the temperature was maintained at 35-37°C. The reaction was carried out for 5-5.5 hours to obtain a modified solution after combined modification;

[0015] (7) Purification: The modified solution was centrifuged and purified using a three-phase centrifuge at a speed of 16,000 to 18,000 r / min to obtain a centrifuge;

[0016] (8) Nanofiltration to remove residual reagents: The centrifuged liquid is filtered to remove polyethylene glycol, and the nanofiltrate is retained;

[0017] (9) Concentration and drying: The nanofiltrate is further concentrated using a double-effect concentrator to obtain a concentrated solution, which is then dried to obtain the medicinal and edible Polygonatum active peptide product.

[0018] Optionally, in step (2), 0.75-1.0% of the weight of the raw material of Polygonatum sibiricum is added. The activity of the amylase is 3.8×10 5 u / g.

[0019] Optionally, in step (3), 1.0-1.5% of the weight of the raw material of Polygonatum sibiricum is added to the neutral protease. The activity of the neutral protease is 3.0×10 5 u / g.

[0020] Optionally, in step (3), the agitator speed is maintained at 40 to 50 r / min.

[0021] Optionally, the activity of the Bacillus licheniformis used in the preparation method is 2000×10 8 ~3000×10 8 CFU / g.

[0022] Optionally, in step (6), the molecular weight of monomethoxypolyethylene glycol is 2000 to 3000 Daltons.

[0023] Optionally, in step (6), the agitator speed is maintained at 35 to 45 r / min.

[0024] Optionally, in step (8), the pore size of the nanofiltration membrane is 0.001 to 0.002 microns.

[0025] Optionally, in step (9), the drying method is spray drying.

[0026] Optionally, the content of Polygonatum sibiricum active peptide is 17-19%.

[0027] The second aspect of the present invention provides a medicinal and edible Polygonatum sibiricum active peptide product prepared by the aforementioned method for preparing the medicinal and edible Polygonatum sibiricum active peptide.

[0028] The third aspect of the present invention provides a method for preparing the aforementioned medicinal and edible Polygonatum active peptide and the use of the medicinal and edible Polygonatum active peptide product prepared by the aforementioned method for preparing nutritional formula foods, health products, special medical foods, and medicines.

[0029] The technical solution of the present invention has the following advantages:

[0030] 1. The present invention utilizes a biotransformation method to extract the active peptides from Polygonatum sibiricum. This method extracts the active ingredients (active peptides) from Polygonatum sibiricum metabolites. Unlike conventional Polygonatum sibiricum extracts extracted using water, alcohol, or organic solutions, this method reduces the toxicity and allergens of traditional Chinese medicine and is easily absorbed by the human body. This method is mild and easy to operate, further improving the safety (non-toxicity) and utility value of the product.

[0031] 2. The biotransformation method of the present invention changes the simple separation and screening technology of traditional Chinese medicine and reduces the risk of antagonism in the combination of Chinese medicines.

[0032] 3. The present invention utilizes dual enzyme (amylase and neutral protease) synergistic technology to thoroughly degrade and separate the active substances of traditional Chinese medicine, thereby improving the product yield. The prior art uses conventional organic solvents to extract the active peptides of Polygonatum sibiricum with an extraction rate of about 15%, while the product yield of the method of the present invention reaches 40-45%, far exceeding the extraction rate of the prior art. The substantial increase in product yield means that the consumption of raw materials and the loss of solvents are reduced. Specifically, the method provided by the present invention first adds amylase to control the molecular weight of Polygonatum sibiricum polysaccharide, making it easier to degrade and extract other active substances of Polygonatum sibiricum, making the enzymatic hydrolyzate easier to filter, and the product yield is higher.

[0033] 4. The present invention uses a combination modification technology, and through the combination modification of monomethoxy polyethylene glycol, the activity of the active peptide of Polygonatum sibiricum is further improved, which is suitable for different requirements of pharmaceutical grade. Specifically, the hydroxyl groups on the molecular chain of monomethoxy polyethylene glycol can be close to each other, and the effect of hydrogen bonding effect is relatively large, which promotes the synthesis of the combination modification of Polygonatum sibiricum peptide and other active substances of Polygonatum sibiricum. On the other hand, the combination modification technology can achieve flexible restriction of cyclized active peptides, stabilize the secondary structure of polypeptides, and improve the affinity of modified peptides to receptors. In addition, monomethoxy polyethylene glycol is used as a modifier, which is better for modifying protein drugs to increase the half-life of the product in vivo, reduce immunogenicity, and also increase the water solubility of the product. The proteins and oligosaccharides in Polygonatum sibiricum are converted into oligopeptides and oligosaccharides. Compared with macromolecular proteins and polysaccharides, monomethoxy polyethylene glycol, as a modifier, has better modifiability, which is beneficial to the synthesis of active substances of Polygonatum sibiricum.

[0034] 5. The preparation method provided by the present invention has a short production cycle, low cost, does not produce any toxic and harmful substances, does not use alcohol, acetone or other organic solvents, is safe, and has no toxic side effects. The prepared product has a good taste, high activity and no odor, and can be widely used in the fields of food, health products, medicines, etc. DETAILED DESCRIPTION

[0035] The present invention will be described in detail below in conjunction with specific embodiments. It should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present disclosure. In the detailed description below, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, it is obvious that one or more embodiments can also be implemented without these specific details. In addition, in the following description, descriptions of known technologies are omitted to avoid unnecessary confusion of the concepts of the present disclosure. In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. Where the manufacturer of the reagents or instruments is not specified, they are conventional products that can be purchased commercially. All terms (including technical and scientific terms) used herein have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used here should be interpreted as having meanings consistent with the context of this specification and should not be interpreted in an idealized or overly rigid manner.

[0036] The "biotransformation method" provided by the present invention adopts the principles of biomimetic research, changing the traditional Chinese medicine extraction method of isolating and screening the active ingredients of traditional Chinese medicine to searching for active ingredients from the metabolites of traditional Chinese medicine. Utilizing bioenzyme technology, the present invention provides a method for producing a Polygonatum sibiricum active peptide that is easier for the body to absorb and utilize, and safer. This method perfectly combines the active peptide with other active substances in Polygonatum sibiricum and can be widely used in foods, health products, special medical foods, and pharmaceuticals.

[0037] In the present invention, "inactivating an enzyme" means terminating an enzyme reaction or terminating enzyme activity.

[0038] The detection method of the Polygonatum sibiricum active peptide in this embodiment is GB / T22729-2008.

[0039] Example 1

[0040] This embodiment provides a method for preparing a medicinal and edible active peptide of Polygonatum sibiricum. The preparation method comprises the following steps:

[0041] (1) Raw material pretreatment: 1000 g of Polygonatum sibiricum was selected and cut into uniform thin slices using a slicer. 10 L of purified water was added and stirred evenly. The mixture was heated to 90°C and steamed for 3 hours to obtain the steamed Polygonatum sibiricum raw material. This step can soften the cell wall structure and release intracellular components.

[0042] (2) Enzymolysis: The steamed Polygonatum sibiricum raw material was cooled to 65°C, 7.5g of amylase was added thereto, the temperature was maintained at 65°C, and the enzymolysis was stirred for 30 minutes to obtain a product after preliminary enzymolysis. The preliminary enzymolysis was able to decompose the starch impurities in the Polygonatum sibiricum. The product after preliminary enzymolysis was cooled to 50°C, 10g of neutral protease was added thereto, the temperature was maintained at 50-53°C, and the enzymolysis was stirred for 3 hours, with the stirrer speed maintained at 40r / min.

[0043] (3) Inactivation of enzyme: Raise the temperature of the enzymatically hydrolyzed product to 90% and inactivate the enzyme for 15 minutes.

[0044] (4) Primary separation: After enzyme inactivation, the liquid is filtered using a decanter centrifuge (solid-liquid separation) and the filtrate is collected. The decanter centrifuge speed is preferably 4000-4500 rpm. The flow rate varies depending on the liquid concentration.

[0045] (5) Active peptide modification: The temperature of the separated filtrate was controlled at 35°C. 1 g of monomethoxypolyethylene glycol was added to the filtrate. The temperature was maintained at 35°C and the stirrer speed was maintained at 35 rpm. The reaction was carried out for 5 hours to obtain a modified solution after combined modification. This step can enhance the stability of the peptide segment.

[0046] (6) Purification: The modified solution is centrifuged and purified using a three-phase centrifuge at a speed of 16,000 r / min to obtain a centrifuge. This step can remove macromolecular impurities and unreacted monomethoxypolyethylene glycol or polyethylene glycol in the modified solution.

[0047] (7) Nanofiltration to remove residual reagents: The centrifuged liquid is filtered through a nanofilter to remove polyethylene glycol, and the nanofiltrate is retained. The pore size of the nanofiltration membrane of the nanofilter is preferably 1 nm.

[0048] (8) Concentration and Drying: The nanofiltrate was further concentrated using a double-effect concentrator to obtain a concentrated solution, which was then spray-dried to obtain the medicinal and edible Polygonatum sibiricum active peptide product. The collected product was concentrated using a double-effect concentrator to a light-shading index of 28-30. The content of the Polygonatum sibiricum active peptide was 17-19%. The distribution of the Polygonatum sibiricum active peptide extracted in this example is shown in the following table.

[0049] Yield content Total % Body absorption rate% Less than 1000 Daltons 95.1 90 2-3 peptide 45 99.8 amino acids 23 29

[0050] Example 2

[0051] The present invention provides another method for preparing a medicinal and edible active peptide of Polygonatum sibiricum, which comprises the following steps:

[0052] (1) Raw material pretreatment: 10 kg of Polygonatum sibiricum was selected and cut into uniform thin slices using a slicer. 110 L of purified water was added thereto and stirred evenly. The mixture was heated to 92° C. and steamed for 3.2 hours to obtain the steamed Polygonatum sibiricum raw material.

[0053] (2) Enzymolysis: The steamed Polygonatum sibiricum raw material was cooled to 66°C, 90g of amylase was added thereto, the temperature was maintained at 66°C, and the enzymolysis was stirred for 40 minutes to obtain a product after preliminary enzymolysis. The product after preliminary enzymolysis was cooled to 52°C, 120g of neutral protease was added thereto, the temperature was maintained at 52°C, and the enzymolysis was stirred for 3.5 hours at a stirrer speed of 45 r / min.

[0054] (3) Inactivation of enzyme: Heat the hydrolyzed product to 92°C and inactivate the enzyme for 13 minutes.

[0055] (4) Primary separation: After inactivation of the enzyme, the product is filtered using a decanter centrifuge (solid-liquid separation) and the filtrate is collected. The rotation speed of the decanter centrifuge is preferably 4000-4500 rpm.

[0056] (5) Modification of active peptides: The temperature of the separated filtrate was controlled at 36°C, 15 g of monomethoxypolyethylene glycol was added to the filtrate, the temperature was maintained at 36°C, the stirrer speed was maintained at 36 r / min, and the reaction was carried out for 5.2 hours to obtain the modified solution after combined modification.

[0057] (6) Purification: The modified solution was centrifuged and purified using a three-phase centrifuge at a speed of 17,000 r / min to obtain a centrifuge solution.

[0058] (7) Nanofiltration to remove residual reagents: The centrifuged liquid is filtered through a nanofilter to remove polyethylene glycol, and the nanofiltrate is retained. The pore size of the nanofiltration membrane of the nanofilter is preferably 1 nm.

[0059] (8) Concentration and drying: The nanofiltrate is further concentrated using a double-effect concentrator to obtain a concentrated solution, which is then spray-dried to obtain the medicinal and edible Polygonatum sibiricum active peptide product. The collected product is concentrated using a double-effect concentrator to a light-shading index of 28-30.

[0060] The distribution of the active peptides extracted from Polygonatum sibiricum in this example is shown in the following table.

[0061] Yield content Total % Body absorption rate% Less than 1000 Daltons 96.5 92 2-3 peptide 46 99.9 amino acids 21 30

[0062] Example 3

[0063] The present invention provides another method for preparing a medicinal and edible active peptide of Polygonatum sibiricum, which comprises the following steps:

[0064] (1) Raw material pretreatment: 500 kg of Polygonatum sibiricum was selected and cut into uniform thin slices using a slicer. 6000 L of purified water was added thereto and stirred evenly. The mixture was heated to 95° C. and steamed for 3.5 hours to obtain the steamed Polygonatum sibiricum raw material.

[0065] (2) Enzymolysis: The steamed Polygonatum sibiricum raw material was cooled to 67°C, 5 kg of amylase was added thereto, the temperature was maintained at 67°C, and the enzymolysis was stirred for 45 minutes to obtain a product after preliminary enzymolysis. The product after preliminary enzymolysis was cooled to 55°C, 75 kg of neutral protease was added thereto, the temperature was maintained at 55°C, and the enzymolysis was stirred for 4 hours at a stirrer speed of 50 r / min.

[0066] (3) Inactivation of enzyme: Heat the hydrolyzed product to 95°C and inactivate the enzyme for 15 minutes.

[0067] (4) Primary separation: After inactivation of the enzyme, the product is filtered using a decanter centrifuge (solid-liquid separation) and the filtrate is collected. The rotation speed of the decanter centrifuge is preferably 4000-4500 rpm.

[0068] (5) Modification of active peptides: The temperature of the separated filtrate was controlled at 37°C, 10 kg of monomethoxypolyethylene glycol was added to the filtrate, the temperature was maintained at 37°C, the stirrer speed was maintained at 37 r / min, and the reaction was carried out for 5.5 hours to obtain the modified solution after combined modification.

[0069] (6) Purification: The modified solution was centrifuged and purified using a three-phase centrifuge at a speed of 18,000 r / min to obtain a centrifuge solution.

[0070] (7) Nanofiltration to remove residual reagents: The centrifuged liquid is filtered through a nanofilter to remove polyethylene glycol, and the nanofiltrate is retained. The pore size of the nanofiltration membrane of the nanofilter is preferably 1 nm.

[0071] (8) Concentration and drying: The nanofiltrate is further concentrated using a double-effect concentrator to obtain a concentrated solution, which is then spray-dried to obtain the medicinal and edible Polygonatum sibiricum active peptide product. The collected product is concentrated using a double-effect concentrator to a light-shading index of 28-30.

[0072] The distribution of the active peptides extracted from Polygonatum sibiricum in this example is shown in the following table.

[0073] Yield content Total % Body absorption rate% Less than 1000 Daltons 98.0 94 2-3 peptide 44 99.9 amino acids 19 28

[0074] It should be noted that the above-mentioned specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the disclosure scope of the present invention and fall within the protection scope of the present invention. Those skilled in the art should understand that the description of the present invention is illustrative and does not constitute a limitation on the claims. The scope of protection of the present invention is defined by the claims and their equivalents. The description of the present invention contains multiple inventive concepts, such as "optionally" and "according to a preferred embodiment", which means that the corresponding paragraph discloses an independent concept, and the applicant reserves the right to file a divisional application based on each inventive concept. Throughout the text, the feature guided by "optionally" is only an optional method and should not be understood as a must-have setting. Therefore, the applicant reserves the right to abandon or delete the relevant preferred features at any time.

Claims

1. A method for preparing a medicinal and edible active peptide of Polygonatum sibiricum, characterized in that: The preparation method comprises the following steps: (1) Raw material pretreatment: selecting a raw material of polygonatum, slicing it, adding purified water 10 to 12 times the weight of the raw material of polygonatum, stirring evenly, and steaming it at 90 to 95° C. for 3 to 3.5 hours to obtain the steamed raw material of polygonatum; (2) Amylase hydrolysis: Cool the steamed Polygonatum sibiricum raw material to 65-67°C, add amylase, maintain the temperature at 65-67°C, and stir for 30-45 minutes to obtain a preliminary enzymatic hydrolysis product; (3) Protease hydrolysis: Cool the product after preliminary hydrolysis to 50-55°C, add neutral protease, maintain the temperature at 50-55°C, and stir for 3.0-4.0 hours; (4) Inactivation of enzyme: heating the hydrolyzed product to 90-95°C and inactivating the enzyme for 10-15 minutes; (5) Primary separation: After inactivation of the enzyme, the filtrate is filtered using a decanter centrifuge and the filtrate is collected; (6) Active peptide modification: The temperature of the separated filtrate was controlled at 35-37°C, and 0.1-0.2% of monomethoxy polyethylene glycol by weight of the raw material of Polygonatum was added, and the temperature was maintained at 35-37°C. The reaction was carried out for 5-5.5 hours to obtain a modified solution after combined modification; (7) Purification: The modified solution was centrifuged and purified using a three-phase centrifuge at a speed of 16,000 to 18,000 r / min to obtain a centrifuge; (8) Nanofiltration to remove residual reagents: The centrifuged liquid is filtered to remove polyethylene glycol, and the nanofiltrate is retained; (9) Concentration and drying: The nanofiltrate is further concentrated using a double-effect concentrator to obtain a concentrated solution, which is then dried to obtain the medicinal and edible Polygonatum active peptide product.

2. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1, characterized in that: In step (2), 0.75-1.0% of the weight of the polygonatum raw material amylase is added.

3. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1 or 2, characterized in that: In step (3), 1.0-1.5% of the weight of the raw material of Polygonatum sibiricum is added as a neutral protease.

4. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1, characterized in that: In step (3), the agitator speed is maintained at 40-50 r / min.

5. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1, characterized in that: In step (6), the molecular weight of monomethoxypolyethylene glycol is 2000 to 3000 Daltons.

6. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1 or 5, characterized in that: In step (6), the agitator speed is maintained at 35 to 45 r / min.

7. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1, characterized in that: In step (8), the pore size of the nanofiltration membrane is 0.001 to 0.002 microns.

8. The method for preparing the edible and medicinal active peptide of Polygonatum sibiricum according to claim 1, characterized in that: In step (9), the drying method is spray drying.

9. The edible and medicinal Polygonatum sibiricum active peptide product prepared by the method for preparing the edible and medicinal Polygonatum sibiricum active peptide according to any one of claims 1 to 8.

10. The method for preparing the edible and medicinal Polygonatum active peptide according to any one of claims 1 to 8, and use of the edible and medicinal Polygonatum active peptide product according to claim 9 in preparing nutritional formula foods, health products, special medical foods, and medicines.