The invention discloses a traditional Chinese medicine paste preparation capable of tonifying kidney and kidney yin for women and a preparation method of the traditional Chinese medicine paste preparation. The traditional Chinese medicine paste preparation comprises semen cuscutae, ginseng, deer-horn glue, glossy privet fruit, prepared rehmannia roots, wolfberry fruit, rhizoma polygonati, herba epimedii, fried rhizoma atractylodis macrocephalae, angelica sinensis, rhizoma curculiginis, Chinese yam, fried eucommia ulmoides, fructus alpiniae oxyphyllae, cynomorium songaricum, asparagus cochinchinensis, bletilla striata, fried astragalus membranaceus, morinda officinalis, dipsacus roots, radix salviae miltiorrhizae and achyranthes roots; the prepared rehmannia roots, the herba epimedii, radix asparagi, eclipta alba, salted rhizoma anemarrhenae, the dipsacus roots, honey-fried licorice roots and longan aril as the main drugs, the ligusticum wallichii powder, dogwood, paenoiae alba, human placenta and poria cocos as the adjuvants, the traditional Chinese medicine paste preparation nourishes kidney essence, tonifies the spleen, regulating the liver, replenishes qi and enriches blood after being eaten by the women; the traditional Chinese medicine paste preparation helps to treat irregular menstruation, oligomenorrhea, amenorrhea and infertility, improves the low ovarian reserve function and premature ovarian failure, promotes the reproductive health of women, delays ageing, and improves the immunological function; the traditional Chinese medicine paste preparation leads to black hair, removes black spots, helps to grow and moisten the hair, leads to a contented look, and helps to improve the looks.