43 results about "In vitro maturation" patented technology

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The present application relates to the field of animal reproduction, and discloses a method for improving the efficiency of in vivo oocyte collection of dairy goats by using aminophylline, which comprises the following steps: step one, superovulation treatment of dairy goats, (1) placing a vaginal suppository; (2) orally administering aminophylline; (3) injecting follicle stimulating hormone (FSH); step two, an in vivo oocyte collection operation method (LOPU) of dairy goats; step three, in vitro culture of oocytes, in vitro maturation (IVM) of oocytes; in vitro fertilization (IVF); and in vitro culture (IVC) of oocytes.The present application has the advantage that, compared with the prior art, the number of oocytes collected from dairy goats is significantly increased by aminophylline treatment, and the problem of low oocyte collection rate is solved.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is tert-butylhydroquinone. By adding 100 [mu] g / mL of TBHQ into a basic culture solution, the first polar body discharge rate of oocytes is remarkably increased to 77.29%, the ROS level is reduced, and the blastocyst rate of in-vitro fertilized embryos is increased. The technology is suitable for breed conservation of livestock genetic resources such as sheep and can replace live livestock transportation to reduce the cost.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The present disclosure provides compositions, kits, and methods for promoting in vitro maturation of cells. The compositions, kits, and methods utilize at least one inhibitor of an epigenetic regulator and at least one agonist of a calcium channel. Suitable applications of the present disclosure include use in maturing and differentiation stem cells into viable mature neuronal cells for use in disease modeling, prevention and treatment. The present disclosure also provides methods of screening compounds that are suitable for promoting in vitro maturation of cells.

The present application relates to the technical field of biological medicine, and more particularly to a culture solution, a kit and an application for in-vitro maturation of aged oocytes. Through in-vitro experiments, the present application adds exogenous stromal cell-derived factor 1, establishes a culture system for in-vitro maturation of aged oocytes, and reveals the molecular mechanism of stromal cell-derived factor 1 playing a role through autophagy, thereby providing a new solution for improving the fertility of aged women and improving the success rate of assisted reproductive technology, and having important clinical application value.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

The present invention relates to a culture medium composition for in vitro maturation of oocytes, comprising piperine as an active ingredient, and an in vitro embryo production method using same. The culture medium composition for in vitro maturation of oocytes, comprising piperine as an active ingredient, of the present invention, can qualitatively improve oocytes by inhibiting reactive oxygen species, and in vitro embryo production efficiency can be increased using in vitro matured oocytes obtained from the medium of the present invention.

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

The invention discloses a method for promoting in-vitro maturation of sheep oocytes. The composition comprises an induction culture solution A and an induction culture solution B. The induction culture solution A and the induction culture solution B comprise a basic culture solution, and the induction culture solution A further comprises AMH; the induction culture solution B also contains FSH; the basic culture solution comprises an M199 basic culture medium, oestrus sheep serum, sodium pyruvate, luteinizing hormone, estradiol and a penicillin and streptomycin mixed solution. According to the composition and the method, through the synergistic effect of FSH treatment and AMH treatment, the maturation rate, the activity and the development potential of sheep oocytes are remarkably improved, so that the success rate of in-vitro fertilization and embryo transplantation is improved, an important scientific basis is provided for sheep reproduction and assisted reproduction technologies, related technologies are further optimized, and the application prospect is wide. The reproduction success rate is improved.

The application belongs to the technical field of cell biology, and provides an oocyte in vitro maturation culture medium additive and application thereof. The experimental results of the application show that coumarin 106 can improve the meiosis process blockage of oocytes, reduce the morphological proportion of abnormal spindle and the proportion of misaligned chromosomes, restore the level of acetylated tubulin, improve microtubule stability, improve the expression level of actin in the spindle of oocytes, improve the misdistribution and expression decrease of mitochondria in oocytes, improve the level of mitochondrial membrane potential, improve the fluorescence signal of cortical granules and specific cortical granule proteases in oocytes, improve the fertilization rate, reduce the level of reactive oxygen species and DNA damage in oocytes, and reduce the occurrence of early apoptosis in oocytes. The culture medium prepared by using coumarin 106 can be used for promoting oocyte in vitro maturation.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

This invention relates to the field of reproductive engineering technology, specifically to the application of rutabagastone in improving the cryopreservation and in vitro maturation of oocytes. This invention is the first to clearly demonstrate that rutabagastone can improve the quality of cryopreservation and in vitro maturation of frozen oocytes. By adding rutabagastone to the cryopreservation solution, thawing solution, and in vitro maturation solution, this invention reduces cryopreservation damage to oocytes, resulting in increased survival rate after thawing, improved mitochondrial function, reduced damage to the oocyte spindle and zona pellucida, reduced oxidative stress levels, and ultimately improved subsequent developmental capacity of frozen oocytes.

The invention discloses a culture solution for promoting in-vitro maturation of bovine oocytes and application of the culture solution, and belongs to the technical field of assisted reproduction. The invention provides the culture solution which is composed of brand new components and is used for promoting in-vitro maturation of the bovine oocytes, support is provided for maturation of the oocytes from multiple aspects of carrier transportation, energy metabolism regulation, signal transduction, extracellular environment and the like, the in-vitro maturation quality of the bovine oocytes can be effectively promoted, and the survival rate of the bovine oocytes is increased. The quality and consistency of nucleus maturation and cytoplasm maturation of oocytes are obviously improved; the cultured mature oocytes are used for in-vitro fertilization, so that the fertilization proportion and the blastocyst hatching rate can be remarkably improved; the provided culture solution is appropriate in component number, clear in proportion, simple and easy to operate in preparation method, short in time consumption, good in repeatability and suitable for batch production, and has a good application prospect in maturation culture of bovine in-vitro oocytes.

The invention provides application of endothelin in oocyte in-vitro maturation and embryo in-vitro culture. New application of endothelin is found, the endothelin can improve the polar body discharge rate of oocytes matured in vitro, promote cytoplasm maturing and improve the normal arrangement proportion of organelles after maturing, certain promotion is provided for improvement of oocyte fertilization, and meanwhile subsequent blastocyst development is promoted. The invention further provides an in-vitro maturation culture solution of the sheep oocytes and an in-vitro maturation method of the sheep oocytes, the in-vitro maturation culture solution contains endothelin, and after the sheep oocytes are treated with the in-vitro maturation culture solution containing the endothelin concentration, the mitochondrial membrane potential level of the oocytes can be remarkably improved, and the survival rate of the sheep oocytes is increased. ROS (reactive oxygen species) in the oocytes is remarkably reduced, and the ATP (adenosine triphosphate) and GSH (glutathione) levels of the oocytes and the normal proportion level of spindles are improved; the maturation rate, the fertilization efficiency, the blastocyst number and the blastocyst rate are remarkably increased, in-vitro maturation of sheep oocytes and in-vitro development of early embryos are promoted, and the production efficiency of the sheep in-vitro embryos is effectively improved.

The invention discloses application of a compound shown in a formula I in promoting in-vitro cultured oocyte development or in-vitro embryo development, and in the formula I, R1 is selected from halogenated alkyl; r2 is selected from H, halogen or C1-6 alkyl; n is selected from 0, 1, 2 or 3. According to the application, the expansion area of cumulus cells in the cumulus-oocyte complex can be increased; the oxidative damage of the cumulus-oocyte complex is relieved; the first polar body discharge rate of the oocytes is improved; the abnormal distribution rate of mitochondria in oocytes is reduced; the membrane potential of mitochondria in the oocyte is improved; and the cleavage rate and blastocyst formation rate of fertilized eggs can be improved, and the apoptosis rate of oocytes can be reduced.

The invention relates to the technical field of gene editing, in particular to a composition and a culture solution for promoting development of bovine gene editing cloned embryos and application of the composition and the culture solution. The composition (DLL3, Vercircon and ML604086) is added into an oocyte in-vitro maturation culture medium, and the DLL3, the Vercircon and the ML604086 cooperate with one another, so that apoptosis and autophagy injury of bovine oocytes are effectively reduced, the cleavage rate and blastocyst rate of embryos are increased, and the method has important practical value for improving the production efficiency and quality of gene editing cloned embryos.

The invention provides a method for improving the in-vitro maturation effect of vitrified bovine GV-stage oocytes, and in the cryopreservation process of the bovine GV-stage oocytes, the developmental ability of the bovine GV-stage oocytes after vitrification is improved by independently or jointly adding ergothioneine (EGT), isocitric acid (ICA), PLCzeta and Scriptaid. Experiments show that by adding EGT and ICA into a pretreatment solution, a freezing solution, an unfreezing solution and an oocyte in-vitro maturation solution and adding PLCzeta and Scriptaid into the unfreezing solution and the oocyte in-vitro maturation solution, damage of freezing to oocytes can be relieved to a great extent.

This disclosure features methods and compositions for the in vitro maturation of oocytes. In particular, this disclosure features methods and compositions relating to the manipulation of multiple ovarian supporting cells (OSCs) to promote the maturation of oocytes in a culture medium. Such methods and compositions are particularly useful in assisted reproductive technology (ART) procedures.

This invention belongs to the field of biomedicine and reproductive health technology, specifically relating to the application of wheat germ extract in the preparation of products that improve the quality of aging oocytes. Through in vitro culture and in vivo gavage models, this invention demonstrates for the first time that wheat germ extract can significantly improve the in vitro maturation rate and in vivo superovulation maturation rate of oocytes from aged mice, significantly reduce intracellular reactive oxygen species levels, inhibit early apoptosis, and reduce oocyte mortality. The optimal in vitro concentration of the wheat germ extract is 0.1%–0.2%, and the optimal gavage dose is 5%. At these concentrations, the improving effect of wheat germ extract is significantly superior to that of spermidine. This invention provides a safe, natural, and long-term-accessible reproductive aging intervention strategy with good scientific value and promising prospects for industrial application.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in promoting in-vitro maturation of sheep oocytes or in-vitro development of sheep embryos. The invention discloses application of alpha-ketoglutaric acid or salt thereof in promoting in-vitro maturation of sheep oocytes or promoting in-vitro development of sheep embryos for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the maturation rate of the sheep oocytes or the in-vitro development efficiency of the sheep embryos, such as cleavage rate, blastocyst rate, hatching blastocyst rate and / or hatching blastocyst cell number. The method can be used for preparing products for promoting in-vitro maturation of sheep oocytes or promoting in-vitro development of sheep embryos.

The invention belongs to the field of reproductive medicine, and particularly relates to application of deaminotyrosine in preparation of a reagent or a medicine for improving the quality of aged oocytes. The invention discloses and provides a mechanism for activating an ERK signal channel by utilizing deaminotyrosine specificity to act on the quality improvement of the aged oocytes for the first time. Experiments prove that the deaminotyrosine can reduce the abnormal form proportion of spindle bodies of oocytes, regulate the transformation of mitochondria to uniform distribution, improve the membrane potential of the mitochondria, reduce the ROS level, improve the maturation rate of the oocytes and improve the development rate of in-vitro fertilized blastocysts. The deaminated tyrosine is an endogenous and symbiotic microbial metabolite of a human body, so that the deaminated tyrosine has extremely high biological safety while improving the quality of aged oocytes, and an effective and safe new means is provided for improving the fertility potential of elderly women and optimizing and assisting a reproductive in-vitro maturation technology.

The invention discloses a novel application of a compound MLN4924 (Pevonedat) in preparation of a medicine for improving oocyte quality and treating related diseases, and belongs to the technical field of biological medicines. PCOS animal model experiments prove that the reproductive endocrine indexes of model animals can be remarkably improved by applying the MLN4924, and the reproductive endocrine indexes are specifically represented by restoring the regularity of an estrus cycle and reducing the levels of testosterone and luteinizing hormone in serum; meanwhile, the fertility is improved, and the birth number is increased. In the aspect of oocyte quality, the treatment can increase the number of normal oocytes in an ovary, improve the first polar body discharge rate of in-vitro maturation of the oocytes and improve the blastocyst formation rate after in-vitro fertilization. A further mechanism research shows that the MLN4924 can inhibit the plasmosis of the TRIM21 in the ovarian granular cells and down-regulate the expression level of the protein of the TRIM21. The results show that the MLN4924 provides a new drug choice for developing related diseases for treating polycystic ovarian syndrome (PCOS) and improving oocyte quality.

The application discloses a culture solution containing lupeol for improving in-vitro maturation efficiency of pig oocytes and application thereof. The application adds lupeol, especially 4 muM lupeol, to an in-vitro maturation culture solution of oocytes, which can effectively improve the oxidation-reduction state of oocytes, significantly reduce the intracellular ROS level, and meanwhile, improve the GSH content, thereby maximally relieving oxidative stress damage in the in-vitro maturation process, significantly improving the maturation rate of pig oocytes in the meiotic metaphase II, and effectively reducing the DNA fragmentation ratio, which indicates that the lupeol has excellent effects of promoting nuclear maturation and protecting genetic material. The lupeol is added to the in-vitro maturation culture solution of oocytes, which is easy to operate and can be applied to the existing culture process, and can stably improve the efficiency and quality of pig in-vitro embryo production, and has important application value for pig germplasm resource preservation, rapid propagation of excellent breeds and development of animal husbandry.

The invention belongs to the technical field of biology, and particularly relates to a composition for improving the developmental potential of oocytes, an in-vitro maturation culture solution and an optimized culture method. And a CCR5 / CXCR3 antagonist. The preparation for in-vitro embryo production contains an Ephrin ligand family and a CCR5 / CXCR3 antagonist, the microenvironment for maturation culture of oocytes is synergistically optimized, further, sesquiterpenol compounds and / or phenylpropane compounds can be added on the basis, and therefore the preparation can be used for preparing the embryos in vitro by means of the synergistic effect of the components. The composition can effectively inhibit the formation of abnormal protein aggregates in oocytes, enhance the ability of proteasomes to remove error protein aggregates, significantly reduce the level of reactive oxygen species (ROS) and increase the content of endogenous antioxidant substances such as glutathione (GSH), so as to reduce the protein toxicity stress, oxidative stress and other dimensions, and thus, the composition can be used for preparing an anti-inflammatory drug. The quality and the fertilization rate of oocytes after in-vitro maturation and the development potential of subsequent embryos are remarkably improved, and the application prospect is wide.

The invention provides a method for improving cow superovulation mother cell quality and application thereof, and aims to improve cow superovulation effect and oocyte quality by improving methionine level of oocyte development microenvironment. The method comprises the following steps: feeding rumen protected methionine to the superovulation dairy cow in vivo, and / or adding methionine into a culture solution when in-vitro maturation culture is carried out on oocytes obtained by superovulation. In the embryo level, the blastocyst forming rate and the embryo quality are remarkably improved, the embryonic development potential is higher, and the quality is better. According to the method, by feeding rumen protected methionine in vivo and / or culturing and adding methionine in vitro, the embryo output effect of cow superovulation is systematically improved, the oocyte quality and the embryo development potential are comprehensively improved from the functional and molecular level, and an innovative solution is provided for efficient embryo production.

The invention discloses a sheep oocyte in-vitro maturation culture solution and a culture method. The sheep oocyte in-vitro maturation culture solution comprises an M199 culture medium, and fetal calf serum with a volume fraction of 10%, a penicillin-streptomycin mixed solution with a volume fraction of 1%, a follicle-stimulating hormone with a volume fraction of 0.02 IU / mL, a luteinizing hormone with a volume fraction of 0.02 IU / mL, 17 beta-estradiol with a volume fraction of 1 [mu] g / mL, cysteine with a volume fraction of 0.1 mmol / L and 13 (S)-HODE with a volume fraction of 200 nmol / L are added into the M199 culture medium. The sheep oocyte in-vitro maturation culture solution contains 13 (S)-HODE, and 13 (S)-HODE can reduce the lipid peroxidation level of sheep oocytes, improve the mitochondrial membrane potential, reduce the oocyte iron ion content, reduce oocyte ferroptosis and improve the blastocyst efficiency after oocyte in-vitro fertilization, can effectively improve the oocyte quality, and can be used for preparing the sheep oocyte in-vitro maturation culture solution. The early embryo development capability is improved.

The invention discloses a method for improving the reproductive performance of cattle by using betaine, and belongs to the technical field of animal reproduction biology. The method comprises applying an effective dose of betaine to bovine oocytes and / or granular cells thereof; the betaine is a natural quaternary ammonium type alkaloid with an anti-oxidation function. According to the invention, it is found through metabonomics that betaine is a key metabolite of cattle high-yield breeding traits for the first time, and it is proved that betaine has double beneficial effects in a cattle breeding system: on a granular cell level, the level of active oxygen in cells induced by hydrogen peroxide can be significantly reduced by adding 5 mM betaine, and oxidative stress can be effectively relieved; on the oocyte level, 0.5 mM betaine is added into an in-vitro maturation and / or embryo culture solution, so that the cleavage rate and blastocyst formation rate of the bovine in-vitro embryo can be remarkably increased. The invention provides a brand-new, safe and effective solution for improving the production efficiency and the reproductive capacity of the bovine in-vitro embryo.

The present application relates to the technical field of gene editing, in particular to a composition for promoting the development of bovine gene editing cloned embryos, a culture solution and application thereof. The present application adds the composition (DLL3, Vercirnon and ML604086) in the in vitro maturation culture medium of oocytes, and effectively reduces the apoptosis and autophagy damage of bovine oocytes through the synergistic cooperation of DLL3, Vercirnon and ML604086, thereby improving the cleavage rate and blastocyst rate of embryos, which has important practical value for improving the production efficiency and quality of gene editing cloned embryos.