The invention provides a method for inducing a high-yield dairy cow to regularly ovulate and increasing the conception rate. The method includes the steps that 1, 35-40 days after the high-yield dairy cows calve, a progestational hormone device is embedded into a dairy cow vagina for the first time; 2, the progestational hormone device is taken out after being embedded for 14-16 days; 3, prostaglandin is injected; 4, 2 days after the prostaglandin is injected, an ovulation promoting hormone is injected; 5, 12-16 hours after the ovulation promoting hormone is injected, artificial insemination is carried out; 6, on the sixth to eighth day after artificial insemination, the progestational hormone device is embedded again, and the further embedding time ranges from 14-16 days. According to the method, the development and ovulation time of follicles in the ovary of the dairy cow can be regulated, detection of oestrus is not needed, artificial insemination is directly carried out, fetus protection medicine is adopted, and the survival rate of embryos is increased. The defects that oestrus of the high-yield dairy cow is not easy to observe is overcome, the oestrus rate and the conception rate of the dairy cow can be increased, the calving interval of the high-yield dairy cow is shortened, breeding benefits of the high-yield dairy cow are increased, and production management of a dairy cow farm is convenient.