18results about How to "Good growth" patented technology

The invention relates to the technical field of agricultural automation, in particular to a multi-layer three-dimensional cultivation automatic transplanting robot for a plant factory, the multi-layer three-dimensional cultivation automatic transplanting robot comprises a cultivation frame, a seedling raising plate is arranged on the side face of the cultivation frame, and the robot comprises a control module, a visual module, a positioning module, a moving module and a clamping module. Comprising a clamping unit and a clamping seat, the clamping seat is fixedly connected with a moving seat, a plurality of lifting units are arranged below the clamping seat, the clamping unit comprises a clamping block detachably connected with the lifting units, a clamping motor is arranged in the clamping block, a plurality of clamping grooves are formed in the lower end face of the clamping block, and clamping jaws are movably arranged in the clamping grooves. A crank is further arranged in the clamping block, the two ends of the crank are connected with the output end of the clamping motor and the clamping jaw respectively, opening and closing actions of the clamping jaw are achieved, by means of the design, the robot can accurately clamp and release the field planting basket, and the transplanting accuracy and consistency are improved.

The invention discloses a transgenic synechocystis strain with enhanced stress resistance and application of the transgenic synechocystis strain in sewage treatment, and belongs to the technical field of microorganisms. According to the invention, the MntH gene is integrated into the genome of the synechocystis PCC6803, so that the transgenic synechocystis PCC6803 is constructed. Through stress of Cr < 6 + > metal ions and hydrogen peroxide, the difference of stress resistance between algae strains before and after transformation is verified. The result shows that the stress resistance of the synechocystis strain is effectively improved by inserting the functional element gene MntH into the genome of the synechocystis PCC6803. Under stress culture of hydrogen peroxide or Cr < 6 + > metal ions, the transgenic algal strain shows better stress resistance, the growth condition is better than that of a wild algal strain, and meanwhile, the content of photosynthetic pigment, protein and the like related to the growth of the transgenic algal strain is higher than that of the wild algal strain. The invention provides a technical means for constructing transgenic algal strains with enhanced stress resistance and researching physiological and metabolic functions of microalgae.

The invention discloses a yak intramuscular precursor fat immortalized cell line and a preparation method thereof. The method comprises the following steps: (1) obtaining yak primary intramuscular precursor fat cells; (2) performing SV40T lentivirus infection on the precursor fat cells in the yak muscle and screening cell strains: performing SV40T lentivirus infection by utilizing a 1 / 2 small-volume infection method to obtain immortalized cells after the precursor fat cells in the yak primary muscle are infected with SV40T, namely immortalized cells for short; puromycin is adopted to screen immortalized cells; and diluting the screened cells by using a limited dilution method, and carrying out enlarged culture to obtain the immortalized cell line. The yak intramuscular precursor fat immortalized cell line constructed by the invention has genetic homogeneity, the heterogeneity influence of cell populations is eliminated, and an in-vitro cell model is provided for subsequent research related to yak intramuscular fat cells.

The application discloses overexpression GmGAI The application belongs to the technical field of plant stress resistance and aims to improve the aluminum stress resistance of soybeans. The application provides an application of GmGAI protein in improving the aluminum stress resistance of soybeans, and the amino acid sequence of the GmGAI protein is shown as SEQ ID NO. 1. The application provides a theoretical basis for the aluminum stress resistance of soybeans.

The application discloses a deep-sea hot spring separated SOD yeast strain and application thereof, and belongs to the technical field of biology. The application provides a Saccharomyces cerevisiae SC-SH18, the strain has good temperature tolerance, the strain has good growth state in the range of 28 DEG C to 45 DEG C, has the best growth effect at 42 DEG C, and can meet the production demand of fermentation under high-temperature conditions. The application provides a high-temperature-resistant SOD enzyme, the SOD enzyme is prepared by fermentation of the Saccharomyces cerevisiae SC-SH18, the SOD enzyme has good heat resistance, has almost no enzyme activity loss after 90 DEG C heat treatment for 120 min, retains more than 80% of enzyme activity after 100 DEG C heat treatment for 30 min, and retains more than 57% of enzyme activity after 100 DEG C heat treatment for 60 min.

This invention relates to the field of in vitro stem cell culture technology, specifically to a culture medium for the proliferation of muscle satellite cells, its culture method, and its application. The culture medium comprises a basal medium, fetal bovine serum, and plant-derived active substances; the plant-derived active substances include at least one selected from apigenin, resveratrol, and quercetin; the volume percentage of fetal bovine serum in the culture medium is 2%-5%. This invention, by adding plant-derived active substances, effectively maintains cell stemness and significantly promotes cell proliferation at low serum concentrations.

The invention belongs to the technical field of plant cultivation, and particularly relates to a Mongolian medicine plant Artemisia stelleriana cutting propagation method, which comprises the following steps: S1, disinfecting Artemisia stelleriana branches; s2, pruning the disinfected Artemisia stelleriana branches, and performing disinfection again to obtain Artemisia stelleriana cuttage segments; s3, treating the cutting sections of the Artemisia stelleriana by using a rooting agent, and cutting the cutting sections of the Artemisia stelleriana subjected to rooting treatment in a matrix for cutting propagation. The cutting propagation method of the Mongolian medicine plant Artemisia stelleriana shows remarkable beneficial effects in the aspects of ecological environment protection, propagation efficiency improvement, germplasm resource enrichment, stress resistance enhancement, economic benefit improvement and the like, and has important popularization and application values.

This invention belongs to the field of plant tissue culture technology, specifically relating to a tissue culture rapid propagation medium and method for rooting *Carex latifolia*. The invention provides a tissue culture rapid propagation medium for rooting *Carex latifolia*, comprising a rooting medium; the rooting medium uses 1 / 2 MS as the basal medium and further includes NAA 0.8 mg·L⁻¹, IBA 1.0 mg·L⁻¹, sucrose 30 g·L⁻¹, and agar 7 g·L⁻¹, with a pH of 5.8-6.0. The invention also provides a method for direct rooting of *Carex latifolia* through tissue culture rapid propagation. Aseptic seedlings of *Carex latifolia* have their outer, weaker leaves removed, and the original root system cut off, retaining 2-3 cm of the above-ground portion. These seedlings are then inoculated into the rooting medium for rooting culture to obtain rooted seedlings. After hardening off the rooted aseptic seedlings, the well-rooted *Carex latifolia* seedlings are transplanted into a substrate to form new, complete plants. The method for rapid propagation of broadleaf sedge by tissue culture provided by this invention has the characteristics of high rooting rate, large average number of roots, well-developed root system, high transplant survival rate, and robust regenerated seedlings.

The invention discloses an intelligent garden irrigation route optimization method and system, and the method comprises the following steps: S1, collecting and preprocessing data, and generating an initial multi-dimensional environment feature vector; s2, inputting the initial multi-dimensional environment feature vector into an adaptive modal aggregation algorithm to generate an irrigation demand intensity index; s3, inputting the irrigation demand intensity index into the multi-scale spatial-temporal feature fusion model to generate an irrigation demand change curve; s4, performing irrigation path planning in combination with an adaptive evolutionary algorithm and upper bound confidence interval tree search, and generating a preliminary irrigation path; s5, transmitting the preliminary irrigation path to a dynamic obstacle detection module driven by semantic segmentation, and dynamically adjusting the irrigation path; s6, the working sequence of irrigation equipment is arranged, and irrigation operation is executed; and S7, inputting the feedback data into a causal reasoning module, and extracting real influence characteristics of irrigation on plant health. According to the method, the intelligent dynamic adjustment of the garden irrigation path is realized by combining methods such as multi-modal perception and upper bound confidence interval tree search.

The invention belongs to the technical field of biological medicine, and particularly relates to application of circular RNA as a marker in detection of hand-foot-and-mouth disease complicated nerve injury. According to the invention, the application of the hsacirc0069335 as the marker in predicting the hand-foot-and-mouth disease complicated nerve injury is found for the first time. In an external verification queue, a serum sample of a patient with a nerve injury symptom does not contain the marker, and the area under an ROC curve of the marker is 1, so that the marker can be used as the marker of the hand-foot-and-mouth disease complicated with the nerve injury and has excellent specificity and sensitivity. Furthermore, it is found that EV71 infection inhibits growth of Schwann cells and damage of myelin sheath formation, resulting in significant injury of a nervous system, and the EV71 infection is related to hsacirc0069335 / miR-29b-3p / PMP22 pathway. The discovery may provide a key molecular target for early warning and treatment of the severe hand-foot-and-mouth disease, so that the development progress of treatment drugs is accelerated.

The invention discloses a method for promoting growth of intercropping crops based on a soil micro food web and application of the method, and belongs to the technical field of crop planting. According to the invention, it is found for the first time that by adding a specific type of bacterial-feeding nematodes into soil, synergistic improvement of growth indexes such as plant height, stem diameter and biomass of two crops can be obviously and stably promoted; the application method is simple and easy to operate, the cost is controllable, and no additional farming equipment or steps are needed; the method is high in environmental compatibility, is particularly suitable for a farmland peanut-corn intercropping system in alkaline and calcareous soil, can effectively improve the crop growth performance, farmland productivity and agricultural product yield and quality, can optimize the soil ecological function, continuously increases the economic benefits of farmers, and has wide application prospects. A brand-new, low-cost and easy-to-popularize candidate scheme is provided for agricultural green sustainable development of the alkaline soil area in North China, and the application prospect is wide.

Medicago sativa MsPUB3.4 III-439 The application of genes in improving plant stress resistance belongs to the technical field of genetic engineering and crop genetic breeding technology. In order to solve the technical problems that there is currently a lack of available alfalfa stress resistance gene resources, and especially the function of PUB family members in abiotic stress and the technical solutions thereof are still blank, experiments prove that, under the conditions of salt stress and / or low temperature stress, the transgenic plants overexpressing the alfalfa MsPUB3.4 III-439 gene exhibit significantly enhanced stress tolerance compared with wild type control plants, indicating that the alfalfa MsPUB3.4 III-439 gene can effectively improve the adaptability of plants to abiotic stress. The present application provides a new gene resource for plant stress resistance breeding, and has important significance for the study of stress resistance improvement and plant growth and development regulation mechanism.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

The present application relates to the cultivation technology field of Paeonia lactiflora, and specifically provides a commercial full-promoting cultivation technology of Paeonia lactiflora. The present application provides a method for Paeonia lactiflora to bloom in off-season. After dwarfing treatment at the Paeonia lactiflora budding stage in April, the swollen flower buds are removed, and the Paeonia lactiflora is subjected to gradient low-temperature treatment in August. The conditions of the gradient low-temperature treatment are as follows: the temperature is controlled at 5-7 DEG C in the first week; the temperature is controlled at 1-5 DEG C in the second week; the temperature is controlled at 0-5 DEG C in the third week; the temperature is controlled at -3-8 DEG C in the fourth week; the temperature is controlled at -10 DEG C in the fifth week; the temperature is controlled at -3-5 DEG C in the sixth week; the temperature is controlled at 0-5 DEG C in the seventh to eleventh weeks, and the Paeonia lactiflora is harvested in December. The method provided by the present application can obtain Paeonia lactiflora fresh flowers in winter, effectively increases the types of fresh flowers in winter, and enriches the winter flower market.

This utility model relates to the field of agricultural planting technology and discloses a citrus planting fixing device, including a fixing rod. The bottom end of the fixing rod is integrally formed with a ground nail for stabilization. Holes for threading ropes are opened on the fixing rod, and the holes are evenly distributed along the path of the fixing rod. This utility model can adapt to different terrains, seedlings with scattered growth or multiple stems by setting fixing rods of various shapes. It can also adapt to seedlings at different growth stages and cover diverse planting scenarios. Combined with the use of PP or PE aging-resistant material for the fixing rod and the integrated molding structure, it avoids corrosion and insect infestation problems caused by rain and other conditions, thus improving durability. The ground nail and multiple holes at the end of the fixing rod can effectively reduce the occurrence of seedling lodging and improve the growth environment and growth status of seedlings.

The application belongs to the field of agricultural biotechnology, and more particularly relates to application of a TasA protein in improving stress resistance of rice. The amino acid sequence of the TasA protein is shown as SEQ ID NO. 1, and the rice tolerance to high temperature and drought stress can be significantly improved. Experiments show that the use of a rice stress resistance regulator containing the TasA protein can effectively alleviate the inhibition of stress on plant growth. Compared with the untreated group, the rice seedlings to which the rice stress resistance regulator containing the TasA protein is applied have a significantly increased plant height, fresh weight and dry weight under high temperature stress, and also show a better growth state under drought stress. The rice stress resistance regulator provided by the application has a simple use method and low cost. The application provides a new technical path for improving crop stress resistance by using an exogenous TasA protein, and is suitable for stress resistance improvement of rice and other crops.

The application discloses a method for rapid proliferation and genetic transformation regeneration of Radix Morindae Officinalis embryogenic callus and belongs to the technical field of plant tissue culture. The method comprises the following steps: firstly, inoculating Radix Morindae Officinalis embryogenic callus into a liquid culture medium to carry out suspension culture to obtain Radix Morindae Officinalis embryogenic callus cell groups; then adding the callus cell groups into a bacterial infection liquid to carry out infection; and then transferring the callus cell groups to a differentiation culture medium to carry out culture to obtain Radix Morindae Officinalis transgenic regenerated plants. The suspension culture medium provided by the application can realize large-scale and rapid proliferation of Radix Morindae Officinalis embryogenic callus, and the embryogenic callus cell groups obtained by the proliferation have a good growth state. In the process of establishing the genetic transformation system of Radix Morindae Officinalis, the method of directly carrying out differentiation culture after co-culture is adopted, the differentiation culture medium does not add resistance screening, and an antibacterial agent, i.e., trimethoprim, is added, so that the transgenic plants are regenerated with high efficiency.

The application discloses a kind of chemical high-salinity wastewater advanced treatment system and processing method, belong to wastewater treatment technical field.The application is sequentially connected by aeration micro-electrolysis cell, oxidation tank, electrodialysis device and bacteria-algae biochemical tank, so as to front " micro-electrolysis + two-stage oxidation " process, rear electrodialysis process and bacteria-algae biochemical degradation process;The application first utilizes aeration micro-electrolysis cell and oxidation tank to strengthen the degradation of organic pollutants, reduce the organic load of rear device, which is conducive to improving the degradation efficiency of subsequent treatment device to pollutants in wastewater;The application is strengthened by rear electrodialysis device, and the removal of organic pollutants in wastewater, while the acid and alkali generated in the process of electrodialysis can remove the residual anion introduced by front-end process simultaneously, effectively solve the problem of large subsequent sludge treatment amount or secondary pollution caused by oxidant addition;The application is rear bacteria-algae biochemical tank, which uses suspended bacteria-algae symbiotic granular sludge, can ensure the treatment effect of nitrogen and phosphorus in wastewater.