8results about How to "Good growth" patented technology

This invention belongs to the field of plant tissue culture technology, specifically relating to a tissue culture rapid propagation medium and method for rooting *Carex latifolia*. The invention provides a tissue culture rapid propagation medium for rooting *Carex latifolia*, comprising a rooting medium; the rooting medium uses 1 / 2 MS as the basal medium and further includes NAA 0.8 mg·L⁻¹, IBA 1.0 mg·L⁻¹, sucrose 30 g·L⁻¹, and agar 7 g·L⁻¹, with a pH of 5.8-6.0. The invention also provides a method for direct rooting of *Carex latifolia* through tissue culture rapid propagation. Aseptic seedlings of *Carex latifolia* have their outer, weaker leaves removed, and the original root system cut off, retaining 2-3 cm of the above-ground portion. These seedlings are then inoculated into the rooting medium for rooting culture to obtain rooted seedlings. After hardening off the rooted aseptic seedlings, the well-rooted *Carex latifolia* seedlings are transplanted into a substrate to form new, complete plants. The method for rapid propagation of broadleaf sedge by tissue culture provided by this invention has the characteristics of high rooting rate, large average number of roots, well-developed root system, high transplant survival rate, and robust regenerated seedlings.

The invention discloses a method for promoting growth of intercropping crops based on a soil micro food web and application of the method, and belongs to the technical field of crop planting. According to the invention, it is found for the first time that by adding a specific type of bacterial-feeding nematodes into soil, synergistic improvement of growth indexes such as plant height, stem diameter and biomass of two crops can be obviously and stably promoted; the application method is simple and easy to operate, the cost is controllable, and no additional farming equipment or steps are needed; the method is high in environmental compatibility, is particularly suitable for a farmland peanut-corn intercropping system in alkaline and calcareous soil, can effectively improve the crop growth performance, farmland productivity and agricultural product yield and quality, can optimize the soil ecological function, continuously increases the economic benefits of farmers, and has wide application prospects. A brand-new, low-cost and easy-to-popularize candidate scheme is provided for agricultural green sustainable development of the alkaline soil area in North China, and the application prospect is wide.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

The present application relates to the cultivation technology field of Paeonia lactiflora, and specifically provides a commercial full-promoting cultivation technology of Paeonia lactiflora. The present application provides a method for Paeonia lactiflora to bloom in off-season. After dwarfing treatment at the Paeonia lactiflora budding stage in April, the swollen flower buds are removed, and the Paeonia lactiflora is subjected to gradient low-temperature treatment in August. The conditions of the gradient low-temperature treatment are as follows: the temperature is controlled at 5-7 DEG C in the first week; the temperature is controlled at 1-5 DEG C in the second week; the temperature is controlled at 0-5 DEG C in the third week; the temperature is controlled at -3-8 DEG C in the fourth week; the temperature is controlled at -10 DEG C in the fifth week; the temperature is controlled at -3-5 DEG C in the sixth week; the temperature is controlled at 0-5 DEG C in the seventh to eleventh weeks, and the Paeonia lactiflora is harvested in December. The method provided by the present application can obtain Paeonia lactiflora fresh flowers in winter, effectively increases the types of fresh flowers in winter, and enriches the winter flower market.

This utility model relates to the field of agricultural planting technology and discloses a citrus planting fixing device, including a fixing rod. The bottom end of the fixing rod is integrally formed with a ground nail for stabilization. Holes for threading ropes are opened on the fixing rod, and the holes are evenly distributed along the path of the fixing rod. This utility model can adapt to different terrains, seedlings with scattered growth or multiple stems by setting fixing rods of various shapes. It can also adapt to seedlings at different growth stages and cover diverse planting scenarios. Combined with the use of PP or PE aging-resistant material for the fixing rod and the integrated molding structure, it avoids corrosion and insect infestation problems caused by rain and other conditions, thus improving durability. The ground nail and multiple holes at the end of the fixing rod can effectively reduce the occurrence of seedling lodging and improve the growth environment and growth status of seedlings.

The application belongs to the field of agricultural biotechnology, and more particularly relates to application of a TasA protein in improving stress resistance of rice. The amino acid sequence of the TasA protein is shown as SEQ ID NO. 1, and the rice tolerance to high temperature and drought stress can be significantly improved. Experiments show that the use of a rice stress resistance regulator containing the TasA protein can effectively alleviate the inhibition of stress on plant growth. Compared with the untreated group, the rice seedlings to which the rice stress resistance regulator containing the TasA protein is applied have a significantly increased plant height, fresh weight and dry weight under high temperature stress, and also show a better growth state under drought stress. The rice stress resistance regulator provided by the application has a simple use method and low cost. The application provides a new technical path for improving crop stress resistance by using an exogenous TasA protein, and is suitable for stress resistance improvement of rice and other crops.

The application discloses a method for rapid proliferation and genetic transformation regeneration of Radix Morindae Officinalis embryogenic callus and belongs to the technical field of plant tissue culture. The method comprises the following steps: firstly, inoculating Radix Morindae Officinalis embryogenic callus into a liquid culture medium to carry out suspension culture to obtain Radix Morindae Officinalis embryogenic callus cell groups; then adding the callus cell groups into a bacterial infection liquid to carry out infection; and then transferring the callus cell groups to a differentiation culture medium to carry out culture to obtain Radix Morindae Officinalis transgenic regenerated plants. The suspension culture medium provided by the application can realize large-scale and rapid proliferation of Radix Morindae Officinalis embryogenic callus, and the embryogenic callus cell groups obtained by the proliferation have a good growth state. In the process of establishing the genetic transformation system of Radix Morindae Officinalis, the method of directly carrying out differentiation culture after co-culture is adopted, the differentiation culture medium does not add resistance screening, and an antibacterial agent, i.e., trimethoprim, is added, so that the transgenic plants are regenerated with high efficiency.

The application discloses a kind of chemical high-salinity wastewater advanced treatment system and processing method, belong to wastewater treatment technical field.The application is sequentially connected by aeration micro-electrolysis cell, oxidation tank, electrodialysis device and bacteria-algae biochemical tank, so as to front " micro-electrolysis + two-stage oxidation " process, rear electrodialysis process and bacteria-algae biochemical degradation process;The application first utilizes aeration micro-electrolysis cell and oxidation tank to strengthen the degradation of organic pollutants, reduce the organic load of rear device, which is conducive to improving the degradation efficiency of subsequent treatment device to pollutants in wastewater;The application is strengthened by rear electrodialysis device, and the removal of organic pollutants in wastewater, while the acid and alkali generated in the process of electrodialysis can remove the residual anion introduced by front-end process simultaneously, effectively solve the problem of large subsequent sludge treatment amount or secondary pollution caused by oxidant addition;The application is rear bacteria-algae biochemical tank, which uses suspended bacteria-algae symbiotic granular sludge, can ensure the treatment effect of nitrogen and phosphorus in wastewater.