411results about How to "Good growth" patented technology

The invention relates to a guided tissue regeneration membrane, which comprises a compact coating and a loose coating, wherein the compact coating is composed of collagen-I or collagen-I composite hyaluronic acid; and the loose coating is composed of collagen-I or collagen-I composite calcium salt. A preparation method of the guided tissue regeneration membrane comprises the following steps of: preparing a collagen solution or a collagen and hyaluronic acid mixed solution by the use of acetic acid; injecting the solution into a self-made die, and drying it in the air to prepare the compact coating; injecting the collagen solution or a mixed liquor of the collagen solution and calcium salt onto the compact coating, and uniformly spreading; precooling the die at ultralow temperature, followed by freeze-drying to prepare the guided membrane with the compact coating and loose coating structure; carrying out vacuum high-temperature crosslinking and chemical crosslinking; and finally cleaning. The regeneration membrane has good histocompatibility and mechanical strength, low antigenicity and strong guided tissue regeneration capability. Its degradation rate in vivo is 3-8 months. The method is easy to operate, is suitable for automatic and continuous large-scale production, and solves the problems of large size of collagen membrane and material uniformity during industrial production.

The invention discloses a serum-free culture medium for mesenchymal stem cells. The serum-free culture medium for the mesenchymal stem cells comprises the following ingredients: fibronectin at the final concentration of 25mu g/ml, basic fibroblast growth factors at the final concentration of 10ng/ml, human epidermal growth factors at the final concentration of 15ng/ml, recombinant human insulin at the final concentration of 1mg/ml, human transferrin at the final concentration of 0.55mg/ml, human blood albumin in a volume ratio of 5 percent, sodium selenite at the final concentration of 0.67mug/ml, L-carnitine at the final concentration of 5mM and resveratrol at the final concentration of 30mu M. When the mesenchymal stem cells are cultured by the serum-free culture medium for the mesenchymal stem cells, animal-derived serum is not contained, so infection risks can be controlled; the L-carnitine and the resveratrol which are added into the serum-free culture medium for the mesenchymalstem cells can effectively improve the growth state of the mesenchymal stem cells; and the growth speed of the mesenchymal stem cells is remarkably improved, and the biological characteristics of themesenchymal stem cells are kept unchanged.

The invention provides a laying hen raising method, and belongs to the technical field of poultry raising. The laying hen raising method comprises the following steps: creating different appropriate raising environments at different stages correspondingly according to the growth law of laying hens; feeding chicken feeds containing different traditional Chinese medicine formulas according to the physical requirement of the laying hens at different stages, so as to enhance the immunity of the laying hens, prevent various diseases, and improve survival rate, egg laying ability and egg quality, wherein the chicken feeds for the laying hens at the chick stage, the poulard stage and the laying hen stage are prepared through adding of edible oil and salt and stir-frying, so as to improve the feeding perfume and taste, promote digestion, enhance appetite of the laying hens, and increase the taking quantities of the chicken feeds; a shelf is arranged in a henhouse, so that the laying hens can crawl and jump; moreover, the stocking time is changed according to different stages, so as to enhance the movement and the exercise of the laying hens, and promote the growth of the laying hens. The laying hens raised according to the method have the advantages that the disease incidence rate is low; the survival rate is high; the laying hens are able-bodied and active; the egg laying ability is high; the egg quality is good.

The invention discloses a construction method of a mandarin fish brain cell system. Aiming at the characteristic of mandarin fish brain cells, the primary cell culture is carried out by separating the brain cells through a serum-contained collagenase digestion method, so that the mandarin fish brain cell system is constructed successfully. Thus, a great quantity of mandarin fish brain source cells can be provided; and the good growth status of sub-culture cells can be maintained. The construction method is strong in repeatability and brings convenience for researching fish virus infection paths and fish virus infection mechanisms, developing viral vaccines and the like.

The invention provides an undifferentiated anti-aging amplification culture medium for human umbilical cord/adipose tissue-derived stem cells. The problem in the prior art that a serum-free medium used for culturing mesenchymal stem cells is easy to differentiate and easily causes aging and apoptosis of adult stem cells is solved. The undifferentiated anti-aging amplification culture medium for human umbilical cord/adipose tissue-derived stem cells comprises cell factors, vitamins and chemical small molecules, and the culture system comprises multiple chemical small molecules and biomacromolecules and has the effects of improving physiological metabolism and proliferation and growth potential of cells, resisting oxidative stress response, scavenging free radicals, preventing DNA mutation, protecting mitochondria, reducing molecular wastes in the cells and activating longevity genes and telomerase.

The invention discloses green health goose forage and a preparation method thereof. The weight parts of composition raw materials of the forage are as follows: 30-40 parts of corn, 15-25 parts of bean cakes, 5-10 parts of wheat bran, 1-3 parts of fish meal, 0.3-0.5 part of lysine, 0.4-0.6 part of methionine, 1-3 parts of stone flour, 0.3-0.5 part of corn gluten meal, 0.2-0.4 part of edible salt, 1-3 parts of a premix compound, 0.1-0.2 part of green tea dust, 2-3 parts of grape seed oil, 0.010-0.015 part of phytase, 0.05-0.15 part of multivitamin, 0.002-0.004 part of wintergreen oil and 3-5 parts of Chinese herbal medicine. The grape seed oil and the green tea dust in the green health goose forage belong to health-care products; the grape seed oil can soothe nerves and reduce activities of the goose; and the green tea contains tea polyphenol which is antioxidant and helpful to health growth of the goose. The forage contains no antibiotics. However, the Chinese herbal medicine has the functions of strengthening stomach to improve digestion, heat-clearing and detoxifying, and the like, is helpful to eliminate endotoxin of the goose, prevents goose diseases, makes the goose grow fast, and increases a feed-to-gain ratio.

The invention discloses lactobacillus plantarum KLDS1001 and application of the lactobacillus plantarum KLDS1001 to inhibition of streptococcus mutans, belonging to the technical field of microorganisms. The lactobacillus plantarum KLDS1001 provided by the invention is collected in China general microbiological culture collection center (CGMCC) with the address of institute of microbiology of Chinese academy of sciences, 1# yard, beichen road west, chaoyang district, Beijing; the collection number is CGMCC No.9066; the collection date is April 16th, 2014. The lactobacillus plantarum KLDS1001 has an inhibition effect on the streptococcus mutans, can serve as an additive to be added to health drugs and such oral health products as chewing gum and is used for oral health; the lactobacillus plantarum KLDS1001 can serve as one of ingredients of oral cleaning products to be added into toothpaste and mouth wash for cleaning the oral cavity and reducing the number of streptococcus mutans in the oral cavity; a probiotic therapy method is developed for preventing and treating oral diseases such as decayed teeth.

The invention discloses a building method of fish head and kidney tissue derived cell lines and the obtained cell lines. The cell lines are obtained by performing a primary culture and a subculture for the treated fish heads and kidney tissues, wherein an employed cell culture medium is prepared by adding fetal calf serum, sodium pyruvate, 2-mercaptoethanol and a human basic fibroblast growth factor into a basic cell culture medium.

The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.

The invention discloses special feed for mandarin fish fries. The special feed comprises raw materials in parts by weight as follows: 20-80 parts of fish meal, 30-80 parts of prawn powder, 15-35 parts of blood worm powder, 2-8 parts of cod-liver oil, 1-3 parts of palm oil, 1-4 parts of citrus oil, 10-30 parts of bone meal, 20-30 parts of sweet corn protein powder, 8-14 parts of corn germ meal, 2-10 parts of bean pulp, 10-30 parts of fermented bioprotein, 5-15 parts of black sesame powder, 8-14 parts of bloodworms, 0.5-3 parts of aloe powder, 0.5-3 parts of lotus leaf powder, 0.1-0.8 parts of cerium citrate, 1-3 parts of methionine, 1-3 parts of threonine, 0.01-0.07 parts of a complex enzyme, 0.01-0.09 parts of microelements, 1-3 parts of Chinese herbal medicines, 2-10 parts of an attractant and 0.5-1.5 parts of multivitamins. With the adoption of the special feed, even if under the condition of manual high-density culture, the fatality rate of the mandarin fish fries is very low, the feed is good in palatability and high in nutritional value, the ratio is reasonable, the cost is low, and fish is delicious.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM)/F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

This invention provides a method for culturing a fluke. Culture introduced elite or wild strong flukes by manpower in clean pool or tank. The culturing process is feeding breeding seedling and feeding seedling in a small pool with anticoagulant-treated fresh blood of cattle, pig, sheep or fowls, or feeding with the packaged treated fresh blood packaged with pig small intestine and put in the pool after bundled to nodular of 2-3cm each membered. This feeding technology is modern; especially this invention adopts noncondensing blood that can guarantee both quality and novelty of the blood, so as to guarantee health and good growing of flukes.

The invention discloses a method for increasing the seedling survival rate of Lonicera sempervirens seeds. The method mainly comprises the steps of collecting seeds, pre-processing the seeds and transplanting the seeds. According to the invention, technologies for breaking seed dormancy, such as low-temperature stratification, growth regulator, and rational utilization of illumination, are comprehensively utilized, so that the seedling root system of the Lonicera sempervirens seeds obtained by utilizing the method disclosed by the invention is extensive; and furthermore, the method has the advantages of being good in growth, rapid in speed and high in survival rate.

The invention discloses special feed for 50-150-day old mandarin fish. The special feed comprises raw materials in parts by weight as follows: 40-80 parts of fish meal, 50-120 parts of krill meal, 30-50 parts of sandworm powder, 10-12 parts of cod liver oil, 0.5-2.5 parts of palm oil, 1.5-3.5 parts of corn oil, 5-18 parts of scallop skirts, 5-18 parts of sweet corn gluten meal, 10-16 parts of maize germ oil, 5-15 parts of cottonseed cakes, 10-18 parts of fermented bioprotein, 8-17 parts of black sesame powder, 10-38 parts of blood worms, 1-3 parts of radix aucklandiae, 1-3 parts of taurine, 0.5-1.5 parts of probiotics, 0.5-1.2 parts of methionine, 0.2-0.8 parts of threonine, 0.05-0.09 parts of compound enzyme, 0.01-0.03 parts of microelements, 2-5 parts of Chinese herbal medicines, 5-10 parts of a phagostimulant and 1-3 parts of vitamins. The product is used for the 50-150-day old mandarin fish, stress caused during feed changing can be reduced, transition between different kinds of feed is facilitated, the feed has good palatability and high nutritional value and is low in cost, the mandarin fish can grow fast if the mandarin fish is fed on the feed, and the fatality rate is small.

The invention discloses a method for breaking through seed dormancy of magnolia biloba seeds, which mainly comprises the steps of seed sorting, soaking treatment of seeds, cold plasma treatment and variable-temperature stratification. By comprehensively utilizing the technologies for breaking through the seed dormancy such as soaking, controlling by a growth regulator, plasma treatment and variable-temperature stratification and the like, the sprouting time of the magnolia biloba seeds can be greatly shortened, seedlings of the magnolia biloba seeds which are obtained through the method respectively have developed root systems and grow fine, the growth speed is fast, and the survival rate is high.

The invention provides a mytilopsis surface modified magnesium alloy and a preparation method and the application thereof. The surface modified magnesium alloy material comprises a magnesium alloy substrate, and a composite coating which is positioned on the surface of the magnesium alloy substrate, wherein the composite coating comprises an inner layer and an outer layer; the inner layer comprises a magnesium-alloy layer shaped double-metal hydroxide and a magnesium hydrate mixing phase; the outer layer comprises a polydopamine layer. According to the mytilopsis surface modified magnesium alloy, the magnesium-alloy layer shaped double-metal hydroxide, the magnesium hydrate mixing phase and the polydopamine layer in the composite coating are capable of synchronously and effectively improving the corrosion resistance of the magnesium alloy, so that cells can survive on a material surface; in addition, polydopamine is high in compatibility relative to the cells, so that the cells can beconveniently adhered to the material surface.

The invention provides a radix dipsaci seed pelletization coating. The seed pelletization coating comprises a fixed layer and an additional layer, wherein the fixed layer is composed of an adhesive, a disintegrating agent, a water-retaining agent and a pelletization additive; the additional layer is composed of a growth regulator, an adhesive, a water-retaining agent, a bactericide, a film forming agent and a pelletization agent. The radix dipsaci seed pelletization coating provided by the invention has favorable drought resistance, and a coated seed can grow better than an uncoated seed under conventional conditions.

The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol/m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.