412results about How to "Good growth" patented technology

The invention relates to a guided tissue regeneration membrane, which comprises a compact coating and a loose coating, wherein the compact coating is composed of collagen-I or collagen-I composite hyaluronic acid; and the loose coating is composed of collagen-I or collagen-I composite calcium salt. A preparation method of the guided tissue regeneration membrane comprises the following steps of: preparing a collagen solution or a collagen and hyaluronic acid mixed solution by the use of acetic acid; injecting the solution into a self-made die, and drying it in the air to prepare the compact coating; injecting the collagen solution or a mixed liquor of the collagen solution and calcium salt onto the compact coating, and uniformly spreading; precooling the die at ultralow temperature, followed by freeze-drying to prepare the guided membrane with the compact coating and loose coating structure; carrying out vacuum high-temperature crosslinking and chemical crosslinking; and finally cleaning. The regeneration membrane has good histocompatibility and mechanical strength, low antigenicity and strong guided tissue regeneration capability. Its degradation rate in vivo is 3-8 months. The method is easy to operate, is suitable for automatic and continuous large-scale production, and solves the problems of large size of collagen membrane and material uniformity during industrial production.

The invention discloses a serum-free culture medium for mesenchymal stem cells. The serum-free culture medium for the mesenchymal stem cells comprises the following ingredients: fibronectin at the final concentration of 25mu g / ml, basic fibroblast growth factors at the final concentration of 10ng / ml, human epidermal growth factors at the final concentration of 15ng / ml, recombinant human insulin at the final concentration of 1mg / ml, human transferrin at the final concentration of 0.55mg / ml, human blood albumin in a volume ratio of 5 percent, sodium selenite at the final concentration of 0.67mug / ml, L-carnitine at the final concentration of 5mM and resveratrol at the final concentration of 30mu M. When the mesenchymal stem cells are cultured by the serum-free culture medium for the mesenchymal stem cells, animal-derived serum is not contained, so infection risks can be controlled; the L-carnitine and the resveratrol which are added into the serum-free culture medium for the mesenchymalstem cells can effectively improve the growth state of the mesenchymal stem cells; and the growth speed of the mesenchymal stem cells is remarkably improved, and the biological characteristics of themesenchymal stem cells are kept unchanged.

The present invention discloses a fermentation plant leaf breeding pig feed, wherein raw materials of the feed comprise, by weight, 160-170 parts of corn, 20-30 parts of soybean meal, 10-20 parts of fermentation plant leaves, 5-8 parts of wheat bran, 8-12 parts of tea leaf residues, 5-10 parts of orange residues, 4-6 parts of slag , 4-6 parts of maifanite, 4-6 parts of a premix, 0.03-0.05 part of methionine, 04-0.5 part of lysine, 0.2-0.3 part of gravy salt, 0.2-0.3 part of allitricin, 0.05-0.15 part of multivitamin, 0.01-0.015 part of phytase, and 3-4 parts of Chinese herbs. According to the present invention, the fermentation plant leaves, the maifanite, the tea leaf residues, the orange residues, the slag and other raw materials are added to the formula, such that palatability and nutrition of the feed and feed intake of the breeding pig can be enhanced, a growth state of the breeding pig can be improved, growth requirements of the breeding pig can be met, pig meat quality can be improved, and good effects are provided for digestion promotion, feed utilization rate improvement, weight increase per day of chicken, economic benefits and the like.

The invention provides a laying hen raising method, and belongs to the technical field of poultry raising. The laying hen raising method comprises the following steps: creating different appropriate raising environments at different stages correspondingly according to the growth law of laying hens; feeding chicken feeds containing different traditional Chinese medicine formulas according to the physical requirement of the laying hens at different stages, so as to enhance the immunity of the laying hens, prevent various diseases, and improve survival rate, egg laying ability and egg quality, wherein the chicken feeds for the laying hens at the chick stage, the poulard stage and the laying hen stage are prepared through adding of edible oil and salt and stir-frying, so as to improve the feeding perfume and taste, promote digestion, enhance appetite of the laying hens, and increase the taking quantities of the chicken feeds; a shelf is arranged in a henhouse, so that the laying hens can crawl and jump; moreover, the stocking time is changed according to different stages, so as to enhance the movement and the exercise of the laying hens, and promote the growth of the laying hens. The laying hens raised according to the method have the advantages that the disease incidence rate is low; the survival rate is high; the laying hens are able-bodied and active; the egg laying ability is high; the egg quality is good.

The invention discloses a constructing method of stem cell breeding and differentiation microenvironment in vitro in the stem cell tissue engineering domain, which comprises the following steps: adopting sodium alginate and polylysine as material to prepare APA microencapsulation ES cell; adding microencapsulation ES cell in the ES growing culture medium; stewing at 37 Deg C in the culture box with 5% CO2; changing culture medium every 2-3d to obtain the product. The invention simplifies the operation to generate high-density and large scale augmentation, which can inhibit or delay the oriental differentiation for ES cell.

The invention discloses a piglet feed capable of strengthening immunity and a preparation method of the piglet feed. The piglet feed is prepared by mixing the ingredients of corn, vinegar residue, extruded soybean, litsea coreana powder, modified sweet potato vine bran, winter daphne and the like with Chinese herbal medicine according to certain parts by weight. A composite acidifier is added in the preparation method of the modified sweet potato vine bran piglet feed disclosed by the invention, so that the appetite of a piglet can be stimulated; food-intake of the piglet is increased; adsorption of the piglet on nutritional ingredients is facilitated; the disease resistance of the piglet is increased, and the quality guarantee period of the feed is long. The common antibiotic is replaced by the ingredients of the Chinese herbal medicine; removing of endotoxin of the piglet is facilitated; a good growth state of the piglet is kept; and the piglet feed can prevent and treat diseases, the piglet grows rapidly, the quality of the pork is improved, and the pork is suitable for being eaten by people.

The invention discloses a construction method of a mandarin fish brain cell system. Aiming at the characteristic of mandarin fish brain cells, the primary cell culture is carried out by separating the brain cells through a serum-contained collagenase digestion method, so that the mandarin fish brain cell system is constructed successfully. Thus, a great quantity of mandarin fish brain source cells can be provided; and the good growth status of sub-culture cells can be maintained. The construction method is strong in repeatability and brings convenience for researching fish virus infection paths and fish virus infection mechanisms, developing viral vaccines and the like.

The invention provides an undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells. The problem in the prior art that a serum-free medium used for culturing mesenchymal stem cells is easy to differentiate and easily causes aging and apoptosis of adult stem cells is solved. The undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells comprises cell factors, vitamins and chemical small molecules, and the culture system comprises multiple chemical small molecules and biomacromolecules and has the effects of improving physiological metabolism and proliferation and growth potential of cells, resisting oxidative stress response, scavenging free radicals, preventing DNA mutation, protecting mitochondria, reducing molecular wastes in the cells and activating longevity genes and telomerase.

The invention discloses green health goose forage and a preparation method thereof. The weight parts of composition raw materials of the forage are as follows: 30-40 parts of corn, 15-25 parts of bean cakes, 5-10 parts of wheat bran, 1-3 parts of fish meal, 0.3-0.5 part of lysine, 0.4-0.6 part of methionine, 1-3 parts of stone flour, 0.3-0.5 part of corn gluten meal, 0.2-0.4 part of edible salt, 1-3 parts of a premix compound, 0.1-0.2 part of green tea dust, 2-3 parts of grape seed oil, 0.010-0.015 part of phytase, 0.05-0.15 part of multivitamin, 0.002-0.004 part of wintergreen oil and 3-5 parts of Chinese herbal medicine. The grape seed oil and the green tea dust in the green health goose forage belong to health-care products; the grape seed oil can soothe nerves and reduce activities of the goose; and the green tea contains tea polyphenol which is antioxidant and helpful to health growth of the goose. The forage contains no antibiotics. However, the Chinese herbal medicine has the functions of strengthening stomach to improve digestion, heat-clearing and detoxifying, and the like, is helpful to eliminate endotoxin of the goose, prevents goose diseases, makes the goose grow fast, and increases a feed-to-gain ratio.

The invention discloses lactobacillus plantarum KLDS1001 and application of the lactobacillus plantarum KLDS1001 to inhibition of streptococcus mutans, belonging to the technical field of microorganisms. The lactobacillus plantarum KLDS1001 provided by the invention is collected in China general microbiological culture collection center (CGMCC) with the address of institute of microbiology of Chinese academy of sciences, 1# yard, beichen road west, chaoyang district, Beijing; the collection number is CGMCC No.9066; the collection date is April 16th, 2014. The lactobacillus plantarum KLDS1001 has an inhibition effect on the streptococcus mutans, can serve as an additive to be added to health drugs and such oral health products as chewing gum and is used for oral health; the lactobacillus plantarum KLDS1001 can serve as one of ingredients of oral cleaning products to be added into toothpaste and mouth wash for cleaning the oral cavity and reducing the number of streptococcus mutans in the oral cavity; a probiotic therapy method is developed for preventing and treating oral diseases such as decayed teeth.

The invention discloses a building method of fish head and kidney tissue derived cell lines and the obtained cell lines. The cell lines are obtained by performing a primary culture and a subculture for the treated fish heads and kidney tissues, wherein an employed cell culture medium is prepared by adding fetal calf serum, sodium pyruvate, 2-mercaptoethanol and a human basic fibroblast growth factor into a basic cell culture medium.

The invention relates to the technical field of intelligent irrigation, and provides an irrigation method and device based on machine learning, and the method comprises the steps: reading environmentdata which comprises weather, soil humidity and plant growth condition data; performing feature engineering processing on the environment data to obtain prediction variable data; sending the prediction variable data to an irrigation effect prediction model, the irrigation effect prediction model being obtained based on labeled historical data training, the label being generated by using a manual method or a machine learning method; obtaining a target variable value output by the irrigation effect prediction model, and generating an irrigation scheme by utilizing the target variable value; andsending a control signal to the water outlet valve with the specified number according to the irrigation scheme. The irrigation opportunity is modeled and controlled based on plant growth conditions and environmental factors, the problem that irrigation cannot be carried out at the optimal opportunity through manual decision making or decision making based on a single environmental factor can be solved, the crop yield can be ensured, and labor and water resources are saved.

The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.

The invention provides a method for starting and efficiently operating a flocculent sludge and granular sludge coexisting autotrophic nitrogen removal system. The method comprises the following steps: firstly, inoculating a sludge from partial nitrification and from an anaerobic ammonium oxidation reactor, gradually increasing the concentration of influent ammonia nitrogen under the condition of intermittent aeration, starting, and changing the aeration mode into a continuous aeration mode by shortening the time of the anaerobic stage, so as to start a one-step autotrophic nitrogen removal system. After the starting is successful, a stable microbial community structure is created through a method of controlling the proportion of the flocculent sludge and the granular sludge, and the reaction period is adjusted according to the operation condition of the system to achieve efficient nitrogen removal of the system. The starting mode provided by the invention is fast and simple, the created system operates stably and efficiently, and the method is of important reference significance for the operation of the one-step autotrophic nitrogen removal system and other flocculent sludge and granular sludge coexisting systems.

The invention discloses special feed for mandarin fish fries. The special feed comprises raw materials in parts by weight as follows: 20-80 parts of fish meal, 30-80 parts of prawn powder, 15-35 parts of blood worm powder, 2-8 parts of cod-liver oil, 1-3 parts of palm oil, 1-4 parts of citrus oil, 10-30 parts of bone meal, 20-30 parts of sweet corn protein powder, 8-14 parts of corn germ meal, 2-10 parts of bean pulp, 10-30 parts of fermented bioprotein, 5-15 parts of black sesame powder, 8-14 parts of bloodworms, 0.5-3 parts of aloe powder, 0.5-3 parts of lotus leaf powder, 0.1-0.8 parts of cerium citrate, 1-3 parts of methionine, 1-3 parts of threonine, 0.01-0.07 parts of a complex enzyme, 0.01-0.09 parts of microelements, 1-3 parts of Chinese herbal medicines, 2-10 parts of an attractant and 0.5-1.5 parts of multivitamins. With the adoption of the special feed, even if under the condition of manual high-density culture, the fatality rate of the mandarin fish fries is very low, the feed is good in palatability and high in nutritional value, the ratio is reasonable, the cost is low, and fish is delicious.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM) / F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

This invention provides a method for culturing a fluke. Culture introduced elite or wild strong flukes by manpower in clean pool or tank. The culturing process is feeding breeding seedling and feeding seedling in a small pool with anticoagulant-treated fresh blood of cattle, pig, sheep or fowls, or feeding with the packaged treated fresh blood packaged with pig small intestine and put in the pool after bundled to nodular of 2-3cm each membered. This feeding technology is modern; especially this invention adopts noncondensing blood that can guarantee both quality and novelty of the blood, so as to guarantee health and good growing of flukes.

The invention discloses streptomyces albidoflavus Actin-1. The streptomyces albidoflavus Actin-1 can grow and propagate with apple tree rot pathogenic bacterium mycelium as nutrition, long-term colonization of biocontrol bacteria on apple tree rot scabs is promoted, a long-term biological prevention and treatment function is achieved, meanwhile, the streptomyces albidoflavus Actin-1 is induced to generate multiple ectoenzyme cell wall hydrolytic enzymes, and pathogenic bacterium cells disintegrate in combination with the enzyme dissolving function; high antibacterial active matter can be generated, the bacteriostasis rate on the apple rot pathogenic bacteria is 89.82%, a good antibacterial effect is achieved on botryosphaeria berengeriana and other pathogenic bacteria, the bacteriostasis rate ranges from 76.08% to 87.10%, and broad spectrum bacteriostasis performance is achieved. The streptomyces albidoflavus Actin-1 is adopted as main biocontrol bacteria for preventing and treating the apple tree rot and other fruit and vegetable pathogenic bacteria, and the advantages of being good in prevention and treatment effect (100%), high in efficiency, low in recurrence rate (0), high in environment adaption capacity, high in stability, not likely to generate resistance to drugs and the like are achieved. Important significance is achieved on improving the prevention and treatment effect on the apple tree rot and other fruit and vegetable pathogenic bacteria, preventing pathogenic bacterium relapse and protecting the environment.

The invention discloses a method for increasing the seedling survival rate of Lonicera sempervirens seeds. The method mainly comprises the steps of collecting seeds, pre-processing the seeds and transplanting the seeds. According to the invention, technologies for breaking seed dormancy, such as low-temperature stratification, growth regulator, and rational utilization of illumination, are comprehensively utilized, so that the seedling root system of the Lonicera sempervirens seeds obtained by utilizing the method disclosed by the invention is extensive; and furthermore, the method has the advantages of being good in growth, rapid in speed and high in survival rate.

The invention discloses special feed for 50-150-day old mandarin fish. The special feed comprises raw materials in parts by weight as follows: 40-80 parts of fish meal, 50-120 parts of krill meal, 30-50 parts of sandworm powder, 10-12 parts of cod liver oil, 0.5-2.5 parts of palm oil, 1.5-3.5 parts of corn oil, 5-18 parts of scallop skirts, 5-18 parts of sweet corn gluten meal, 10-16 parts of maize germ oil, 5-15 parts of cottonseed cakes, 10-18 parts of fermented bioprotein, 8-17 parts of black sesame powder, 10-38 parts of blood worms, 1-3 parts of radix aucklandiae, 1-3 parts of taurine, 0.5-1.5 parts of probiotics, 0.5-1.2 parts of methionine, 0.2-0.8 parts of threonine, 0.05-0.09 parts of compound enzyme, 0.01-0.03 parts of microelements, 2-5 parts of Chinese herbal medicines, 5-10 parts of a phagostimulant and 1-3 parts of vitamins. The product is used for the 50-150-day old mandarin fish, stress caused during feed changing can be reduced, transition between different kinds of feed is facilitated, the feed has good palatability and high nutritional value and is low in cost, the mandarin fish can grow fast if the mandarin fish is fed on the feed, and the fatality rate is small.

The invention discloses a method for breaking through seed dormancy of magnolia biloba seeds, which mainly comprises the steps of seed sorting, soaking treatment of seeds, cold plasma treatment and variable-temperature stratification. By comprehensively utilizing the technologies for breaking through the seed dormancy such as soaking, controlling by a growth regulator, plasma treatment and variable-temperature stratification and the like, the sprouting time of the magnolia biloba seeds can be greatly shortened, seedlings of the magnolia biloba seeds which are obtained through the method respectively have developed root systems and grow fine, the growth speed is fast, and the survival rate is high.

The invention discloses a constructing method and application of a koi brain cell line. The constructing method includes: placing koi brain tissue in digestive liquid A containing collagenase and trypsin for digestion; collecting cells; adding into digestive liquid b containing EDTA-4Na for digestion; performing primary culture and secondary culture after the cells which are digested are collected to obtain the koi brain cell line. Aiming at features of koi brain tissue cells, a digestion method combining collagenase with trypsin is adopted to separate tissue cells for primary culture, the koi brain cell line is constructed successfully and continuously transferred to more than 100 generations already, and a lot of koi brain source cells can be provided; the cells can maintain good growing state, so that the cells can be freeze-stored. The koi brain cell line is sensitive to koi herpes viruses with cytopathy occurring after being exposed to the viruses, and proliferated virus particles can be found through electron microscope observation. And the koi brain cell line can be directly applied to pathogenic characteristic study and vaccine development.

The invention provides a mytilopsis surface modified magnesium alloy and a preparation method and the application thereof. The surface modified magnesium alloy material comprises a magnesium alloy substrate, and a composite coating which is positioned on the surface of the magnesium alloy substrate, wherein the composite coating comprises an inner layer and an outer layer; the inner layer comprises a magnesium-alloy layer shaped double-metal hydroxide and a magnesium hydrate mixing phase; the outer layer comprises a polydopamine layer. According to the mytilopsis surface modified magnesium alloy, the magnesium-alloy layer shaped double-metal hydroxide, the magnesium hydrate mixing phase and the polydopamine layer in the composite coating are capable of synchronously and effectively improving the corrosion resistance of the magnesium alloy, so that cells can survive on a material surface; in addition, polydopamine is high in compatibility relative to the cells, so that the cells can beconveniently adhered to the material surface.

The invention relates to an algae cultivation technology suitable for shallow sea huge-wave torrential-current sea areas, in particular to algae wind-wave resisting rope sinking type cultivation facility and method, which aim at solving the problem that raft culture algae seedlings are seriously lost in larger wind-wave sea area. The facility is provided with a seedling rope, a drop stone and a floating ball; one end of the seedling rope is tied with the floating ball or other floating bodies, and the other end of the seedling rope is tied with the drop stone or objects made of other materials; and the seedling rope is fixed with algae seedlings. When the algae is thrown, the seedling rope is orderly arrayed on a boat; when the boat reaches a cultivation water area, the boat speed is keptconstant and the algae seedlings are sequentially thrown; and the algae is harvested by a multitooth harrow or partly harvested by divers under water, thereby multiple cultivation can be realized. Theinvention not only ensures that the algae seedling rope completely keeps away from the influence of sea water, wind and wave on the upper layer, avoids the loss of algae cultivation caused by severeweather, but also ensures that the algae can be cultivated in open sea areas or sea areas on the periphery of islands with smoother water current exchange and clean water quality and can be suitable for shallow sea cultivation of small-medium economic algae.

The invention provides a radix dipsaci seed pelletization coating. The seed pelletization coating comprises a fixed layer and an additional layer, wherein the fixed layer is composed of an adhesive, a disintegrating agent, a water-retaining agent and a pelletization additive; the additional layer is composed of a growth regulator, an adhesive, a water-retaining agent, a bactericide, a film forming agent and a pelletization agent. The radix dipsaci seed pelletization coating provided by the invention has favorable drought resistance, and a coated seed can grow better than an uncoated seed under conventional conditions.

The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol / m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.

The invention relates to a method for producing rotavirus vaccines in large scale by utilizing a bioreactor, and in particular relates to a method for culturing rotavirus inactivated vaccines in large scale by utilizing a 75L bioreactor. The method comprises the following steps: (1) carrying out reviving and passage on Vero cells; (2) collecting the cells and inoculating the cells into the bioreactor; (3) culturing the cells in the bioreactor; (4) changing the liquid and washing the cells; (5) activating and infecting rotaviruses; and (6) carrying out maintenance culture and harvesting on the rotaviruses. The method aims at overcoming the defect that in the large-scale production, the rotaviruses can not release cells easily, so that the obtained viruses are low in toxicity titer, and the inactivated rotavirus vaccines with high titer and high yield are obtained; the method also aims at overcoming the defect that in the large-scale production, the adsorption force and infection force of the rotaviruses are poor, and thus the yield and quality of the rotaviruses are greatly improved.