69results about How to "Good growth" patented technology

The invention relates to the technical field of agricultural automation, in particular to a multi-layer three-dimensional cultivation automatic transplanting robot for a plant factory, the multi-layer three-dimensional cultivation automatic transplanting robot comprises a cultivation frame, a seedling raising plate is arranged on the side face of the cultivation frame, and the robot comprises a control module, a visual module, a positioning module, a moving module and a clamping module. Comprising a clamping unit and a clamping seat, the clamping seat is fixedly connected with a moving seat, a plurality of lifting units are arranged below the clamping seat, the clamping unit comprises a clamping block detachably connected with the lifting units, a clamping motor is arranged in the clamping block, a plurality of clamping grooves are formed in the lower end face of the clamping block, and clamping jaws are movably arranged in the clamping grooves. A crank is further arranged in the clamping block, the two ends of the crank are connected with the output end of the clamping motor and the clamping jaw respectively, opening and closing actions of the clamping jaw are achieved, by means of the design, the robot can accurately clamp and release the field planting basket, and the transplanting accuracy and consistency are improved.

The invention discloses an intelligent beef cattle breeding and feed management monitoring system in the technical field of cattle breeding. Comprising an NFC label used for distinguishing cattle, a camera used for shooting the overall state of the cattle, a weighing instrument used for being placed on one side of a trough to weigh the cattle, a temperature and humidity sensor and an accurate feeding module. Calculating and preparing a feed formula and total amount; and the intelligent monitoring module is used for receiving the feed intake and the cattle state video data of the cattle identified and monitored by the camera, and automatically judging the overall state of the cattle according to the feed intake and the cattle state video data. The system is scientific and reasonable, the system makes a scientific and reasonable mating plan, and ensures that cattle can obtain appropriate nutrition supply through data sharing and cooperative work, and effective linkage of breeding and feed management is achieved.

The invention discloses a marine fish myoblast in-vitro proliferation method. A basal culture medium is used as a background culture medium of a culture medium; the culture medium contains IGF2 protein with the final concentration of 20-200 ng / mL; the IGF2 protein is derived from plectropomus leopardus. The culture medium is used for culturing the passage cells to obtain the myoblasts proliferated in vitro of the plectropomus leopardus, the in-vitro culture of the myoblasts of the plectropomus leopardus is successfully realized, the number of the myoblasts reaches 30000, and the in-vitro proliferation rate reaches 87%. The invention provides raw materials for exploring the self function of myoblasts and gene regulation and control mechanism, provides preconditions for an in-vitro certification experiment for further exploring the influence of the myoblasts of marine fishes on the growth speed, and also lays a foundation for improved variety breeding of the marine fishes.

The invention relates to the technical field of plant cultivation, in particular to a method for regulating and controlling precocious flowering of safflower by using a photoperiod. According to the method, safflower seedlings are cultured in a photoperiod environment of 20-hour illumination and 4-hour darkness, and flowering safflower plants are obtained. By adopting the method provided by the invention, the flower bud of the safflower can be successfully induced to differentiate in advance, the safflower can be promoted to bloom in advance, the growth state of the safflower is relatively good, and the method is an optimal photoperiod treatment condition for promoting the growth and development of the safflower and blooming in advance. The safflower treated by the method provided by the invention only needs 84 days from sowing to flowering, which is about 5 months earlier than that sown in autumn in the south in actual production, the growth cycle of the safflower is greatly shortened, and the plant height of the safflower plant is also higher than that of the safflower plant treated under the existing photoperiod condition and is two times that of the safflower plant treated under the photoperiod condition of 12h / 12h. The obvious difference is realized.

The invention discloses an intelligent seed and fertilizer integrated seeding unit, belongs to the technical field of seeding machine equipment, and particularly relates to a stirring, fertilizing and seeding integrated machine which comprises a rack, an advancing device, a ditching and soil covering device, a fertilizer discharging device, a seeding device and a control system. According to the seeding machine, the seeding rate can be adjusted, fixed-point and quantitative discharging is realized, a mode of seeding first and then fertilizing or a mode of fertilizing first and then seeding can be selected according to specific conditions, the problems of complicated seeding rate adjustment, miss seeding, uneven seeding rate, inconsistent seeding depth and the like during seeding operation of a traditional mechanical seeding machine are solved, fine planting of various crops and adaptation to different land parcels is realized, and the seeding efficiency is improved. The soil covering device and the soil leveling device of the seeder are suitable for more complex terrains, irregular terrains between hills or mountainous regions can be fully utilized, the soil covering device and the soil leveling device of the seeder are improved and designed, and by adding a rigid spring and an adjustable mechanical structure, the adjusting range is expanded, and the applicability of the soil covering device and the soil leveling wheel is improved.

The invention relates to the technical field of intelligent weeding, and discloses a multi-sensor information fusion orchard intelligent inter-plant weeding system which comprises a sensing data module and an intelligent control module. According to the system, image data of all paths and operation data of all time points are obtained through a sensing data module and classified to form a data set, an intelligent control module establishes a two-dimensional coordinate system in an equal proportion mode, the inter-plant distance of each fruit tree is analyzed, gridding processing is carried out according to the two-dimensional coordinate system, the trunk diameter of each fruit tree is calculated, and the tree diameter of each fruit tree is calculated. The growth states of all fruit trees are mastered in real time to analyze the moving trajectory of the weeding robot, the moving trajectory is precisely planned, the applicability is high, the intelligent control module analyzes the coverage rate of weeding operation and judges the growth states of the fruit trees and the growth states of weeds, and if the coverage rate is reduced under the condition that the predicted duration is not changed within the monitoring period, the weeding robot is started. If the average cutting speed is reduced, the growth state of weeds is vigorous, the weeding operation frequency needs to be increased, and the weeding effect is accurately evaluated to be good.

The invention discloses a flower promoting cultivation method for multiple flowering of hybrid chimonanthus praecox, and belongs to the technical field of garden plant cultivation. The flower promoting cultivation method for multiple flowering of the hybrid chimonanthus praecox comprises the following steps: S1, applying an organic fertilizer and calcium superphosphate as a base fertilizer in October to November in autumn and winter; s2, performing updating pruning from December in winter to February in the next year; s3, applying a compound fertilizer after sprouting in spring, and immediately watering after applying the compound fertilizer; s4, pruning after flower bud differentiation and before flowering; and S5, in May to August, branches with flowers bloomed or thick and strong branches with lower branches with flowers bloomed are selected and pruned for 2-3 times in total, after pruning, compound fertilizer with high phosphorus and potassium element content is applied once every 10-15 days, and the concentration is 0.5-2%. According to the flower promoting cultivation method for multiple flowering of the hybrid chimonanthus praecox in one year, multiple flowering of the hybrid chimonanthus praecox can be achieved, the flowering phase can be directly continued from May to September, the flowering time of the hybrid chimonanthus praecox is prolonged, and the garden application landscape value is remarkably improved.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for improving osteothione as well as a preparation method and application thereof. The invention provides a composition for improving osteothione, which is prepared from the following components in parts by mass: 500 to 600 parts of folium cortex eucommiae, 300 to 350 parts of male flower of cortex eucommiae, 300 to 350 parts of wine-processed rhizoma polygonati, 300 to 350 parts of radix fici simplicissimae, 200 to 250 parts of maca, 90 to 120 parts of natto, 60 to 80 parts of calcined oyster shell and 0.5 to 1 part of ossein. The composition can increase the number of bone trabecula, increase bone density, bone volume fraction, the number of bone trabecula, bone trabecula thickness and bone surface area-to-tissue volume ratio, lower bone surface area-to-bone volume ratio and bone trabecula separation degree, enhance osteoblast activity, inhibit osteoclast maturation and differentiation and relieve inflammation, and is high in safety performance. In conclusion, the composition provided by the invention has a certain improvement effect on osteoporosis.

The present invention discloses a method for in vitro proliferation of marine fish myoblasts, wherein the culture medium uses a basal culture medium as a background culture medium; the culture medium contains an IGF2 protein with a final concentration of 20-200 ng / mL; and the IGF2 protein is derived from leopard gill perch. The present invention uses the above culture medium to culture passage cells to obtain myoblasts that proliferate in vitro of leopard gill perch, successfully achieving in vitro culture of leopard gill perch myoblasts, and the number of myoblasts reaches 30,000, and the in vitro proliferation rate reaches 87%. Raw materials are provided to explore the functions of myoblasts themselves and the gene regulation mechanism, and the prerequisite for in vitro verification experiments is provided for further exploring the influence of marine fish myoblasts on the growth rate, and also lays a foundation for the breeding of marine fish.

The invention discloses a transgenic synechocystis strain with enhanced stress resistance and application of the transgenic synechocystis strain in sewage treatment, and belongs to the technical field of microorganisms. According to the invention, the MntH gene is integrated into the genome of the synechocystis PCC6803, so that the transgenic synechocystis PCC6803 is constructed. Through stress of Cr < 6 + > metal ions and hydrogen peroxide, the difference of stress resistance between algae strains before and after transformation is verified. The result shows that the stress resistance of the synechocystis strain is effectively improved by inserting the functional element gene MntH into the genome of the synechocystis PCC6803. Under stress culture of hydrogen peroxide or Cr < 6 + > metal ions, the transgenic algal strain shows better stress resistance, the growth condition is better than that of a wild algal strain, and meanwhile, the content of photosynthetic pigment, protein and the like related to the growth of the transgenic algal strain is higher than that of the wild algal strain. The invention provides a technical means for constructing transgenic algal strains with enhanced stress resistance and researching physiological and metabolic functions of microalgae.

The invention discloses a high and cold gobi area soil attribute improvement method, which belongs to the technical field of soil improvement, and is realized through five steps of sampling analysis, improvement and allocation, sowing, rotary tillage and water spraying. According to the improvement method, the pH value, salinization and organic matter content of the soil in the high and cold gobi area are effectively improved by reasonably blending the materials such as the organic fertilizer and the mineral soil conditioner, and the sustainability of the soil is enhanced. The improved soil not only can support current crop growth, but also provides a more stable and long-term basis for future agricultural production. By improving the soil fertility, the crop yield and the growth quality, the improved soil brings higher economic benefits to farmers. According to the method, by adjusting the pH value and reducing the salinity of the soil, the biological availability of the soil is improved, the soil salinization process is effectively restrained, the long-term negative influence of salinization on agricultural production is avoided, and soil health can be kept.

The invention discloses a yak intramuscular precursor fat immortalized cell line and a preparation method thereof. The method comprises the following steps: (1) obtaining yak primary intramuscular precursor fat cells; (2) performing SV40T lentivirus infection on the precursor fat cells in the yak muscle and screening cell strains: performing SV40T lentivirus infection by utilizing a 1 / 2 small-volume infection method to obtain immortalized cells after the precursor fat cells in the yak primary muscle are infected with SV40T, namely immortalized cells for short; puromycin is adopted to screen immortalized cells; and diluting the screened cells by using a limited dilution method, and carrying out enlarged culture to obtain the immortalized cell line. The yak intramuscular precursor fat immortalized cell line constructed by the invention has genetic homogeneity, the heterogeneity influence of cell populations is eliminated, and an in-vitro cell model is provided for subsequent research related to yak intramuscular fat cells.

The invention relates to the technical field of soilless culture, and particularly discloses a vegetable soilless culture seedling culture and a seedling culture method thereof, and the seedling culture method of the vegetable soilless culture seedling culture comprises the following steps: cleaning and disinfecting vegetable seeds, soaking the vegetable seeds in a soaking solution, carrying out dark treatment, planting the vegetable seeds in a soilless culture substrate, and culturing to obtain vegetable seedlings. The soilless culture substrate comprises a solid substrate and a liquid substrate, the solid substrate comprises modified rock wool, modified perlite and gravel, and the liquid substrate comprises vegetable crop rotten vegetables, a leavening agent, brown sugar, a PH regulator and a heavy metal ion chelating agent. The vegetable soilless culture substrate prepared by the invention is good in water-retaining property, has a certain prevention and treatment effect on diseases and insect pests, and can solve the problem of heavy metal pollution in seedling culture, and the seedling culture method used by the invention has the advantages that vegetable seeds are high in germination rate, strong in disease and insect pest resistance and good in growth state.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a method for inducing Hangzhou chrysanthemum leaf adventitious bud regeneration by using a potato extract, which sequentially comprises the following steps: cultivating Hangzhou chrysanthemum stem segments with buds into Hangzhou chrysanthemum aseptic seedlings with at least four leaves; the method comprises the following steps: taking down 3-4 leaves from the top of a sterile seedling of Hangzhou chrysanthemum, taking a leaf disc from each leaf close to a petiole by using a puncher, immediately inoculating the leaf disc onto an adventitious bud induction culture medium, and culturing in a plant tissue culture room; and obtaining Hangzhou chrysanthemum leaf adventitious buds. According to the method, the leaf disc adventitious bud induction time is short, the growth state is good, subsequent normal rooting, transplanting and breeding can be carried out, and a foundation is laid for successfully establishing a Hangzhou chrysanthemum genetic transformation system.

The invention discloses a cell culture medium additive as well as a preparation method and application thereof. The cell culture medium additive comprises the following components: beta-sitosterol, sericin, hydrolyzed corn starch, vitamins and a liquorice extract. Cortical neurons cultured by the cell culture medium additive provided by the invention are high in survival rate and good in growth state, and the purity reaches 90% or above; all the components in the cell culture medium additive provided by the invention have a synergistic effect, so that the cell culture medium additive plays a role in promoting the survival, growth and development of neurons and can prevent the neurons from being damaged to die; meanwhile, the antibacterial effect can be achieved without adding antibiotics, and adverse effects of the antibiotics on growth and differentiation of nerve cells are avoided; when the cell culture fluid provided by the invention is used for culturing cells, the experimental operation can be simplified, the cost can be reduced, and the stability and repeatability of experimental results can be improved.

The invention discloses a dual-responsive hydrogel with a three-dimensional topological structure, a synthesis method and a use thereof, and belongs to the technical field of biomedical materials. The invention uses a polymer material containing amino and phenylboronic acid functional groups, a polymer material containing aldehyde and hydroxyl functional groups and a magnetic nanomaterial as basic raw materials, prepares a pH / glucose dual-responsive hydrogel through an in-situ crosslinking reaction and hydrogen bond and charge interaction, and then orients the magnetic nanomaterial in the pH / glucose dual-responsive hydrogel through an external magnetic field to prepare a dual-responsive hydrogel with a three-dimensional topological structure. The hydrogel can encapsulate drugs and cells at the same time, and continuously and controllably release drugs, and the cells are in a good growth state, so the hydrogel is an ideal target hydrogel.

The utility model discloses an ecological indoor air purification device, and relates to the technical field of indoor air purification. The technical problem that an existing indoor air purification device is difficult to achieve ecological purification and meanwhile consumables are reduced is solved. According to the technical scheme, the ecological indoor air purification device comprises an air purification device body and further comprises a limiting frame, a transparent glass cover, an air outlet, a maintenance assembly, a receptacle, an air supply assembly and an auxiliary assembly; according to the ecological indoor air purification device, automatic irrigation can be provided through the maintenance assembly so as to help green plants to keep a good growth state, indoor air can be conveniently guided to the position near the green plants through the air supply assembly, air purification can be further achieved through the auxiliary assembly, and compared with a traditional purification mode, the ecological indoor air purification device has the advantages of being convenient to use. The device adopts a natural biological purification mode of green plants to improve the indoor air quality, not only has an ornamental value, but also realizes ecological cyclic utilization and effectively reduces the filter material cost at the same time.

The invention discloses a construction method of a lateolabrax japonicus kiss cell line and application of the lateolabrax japonicus kiss cell line in toxicity assessment of environmental pollutant microplastics. The lateolabrax japonicus kiss cell line is constructed by the following method: under a sterile condition, taking lateolabrax japonicus kiss tissues, cutting the lateolabrax japonicus kiss tissues into pieces, and performing enzyme digestion to obtain primary cells; after the primary cells are subjected to enzyme digestion, complete medium resuspension cells are taken and inoculated into a cell culture bottle, and constant-temperature culture and passage are performed at 28 DEG C. The lateolabrax japonicus kiss cell line can preserve lateolabrax japonicus genetic resources at the cellular level, preservation is simple and convenient, an in-vitro research system is provided, a platform is built for lateolabrax japonicus cell molecular biology research, and the method is suitable for large-scale popularization and application. Meanwhile, the material can also be used as an important material and model for in-vitro researches of molecular genetics, biology, environmental toxicology and the like. According to the method, the cells of the lateolabrax japonicus kiss cell line are cultured in the culture medium containing the environmental pollutant microplastics, and the cell condition is observed, so that the toxicity of the environmental pollutant microplastics can be evaluated more accurately.

The invention provides a lactic acid bacteria preparation capable of being preserved for a long time at normal temperature and a preparation method thereof, and relates to the technical field of microorganisms, the lactic acid bacteria preparation successfully realizes long-time stable preservation at normal temperature through an optimized strain combination, an elaborately-prepared protective agent and an advanced preparation process, and the lactic acid bacteria preparation can be stored for a long time at normal temperature. Compared with a lactic acid bacteria preparation in the prior art that the activity is greatly reduced in a short time at normal temperature and the storage life is limited, the preparation disclosed by the invention can be stably stored for at least 9-15 months, so that the shelf life of the product is greatly prolonged, the high survival rate and stability of lactic acid bacteria are guaranteed, and the product quality is improved by accurately controlling the activation and culture conditions of the strain. According to the present invention, the temperature, the time and the bacterial liquid concentration are determined, the good growth state and the good activity of the lactic acid bacteria at the initial stage are ensured, the optimized bacterial strain combination enhances the product performance, and the specific combination of the lactobacillus plantarum, the lactobacillus acidophilus and the bifidobacterium is adopted to achieve the synergistic effect of the different bacterial strains;

The application discloses overexpression GmGAI The application belongs to the technical field of plant stress resistance and aims to improve the aluminum stress resistance of soybeans. The application provides an application of GmGAI protein in improving the aluminum stress resistance of soybeans, and the amino acid sequence of the GmGAI protein is shown as SEQ ID NO. 1. The application provides a theoretical basis for the aluminum stress resistance of soybeans.

The invention relates to the technical field of plant genetic engineering, in particular to application of an IDD gene and a method for reinforcing phytoremediation of heavy metal contaminated soil based on IDD transgene. The application of the IDD gene is to repair heavy metal contaminated soil, and the IDD gene comprises at least one of an IDD14 gene, an IDD15 gene and an IDD16 gene. The method for repairing the heavy metal contaminated soil comprises the step of planting the IDD transgenic strengthening plants on the heavy metal contaminated soil, wherein the IDD transgenic strengthening plants comprise at least one of an IDD14 transgenic plant, an IDD15 transgenic plant and an IDD16 transgenic plant. According to the invention, three gene modification technologies of IDD14, IDD15 and IDD16 are adopted, so that the tolerance and absorption capacity of plants to heavy metals are improved, and the effect of repairing soil by the plants is enhanced.

The application discloses a deep-sea hot spring separated SOD yeast strain and application thereof, and belongs to the technical field of biology. The application provides a Saccharomyces cerevisiae SC-SH18, the strain has good temperature tolerance, the strain has good growth state in the range of 28 DEG C to 45 DEG C, has the best growth effect at 42 DEG C, and can meet the production demand of fermentation under high-temperature conditions. The application provides a high-temperature-resistant SOD enzyme, the SOD enzyme is prepared by fermentation of the Saccharomyces cerevisiae SC-SH18, the SOD enzyme has good heat resistance, has almost no enzyme activity loss after 90 DEG C heat treatment for 120 min, retains more than 80% of enzyme activity after 100 DEG C heat treatment for 30 min, and retains more than 57% of enzyme activity after 100 DEG C heat treatment for 60 min.

The invention relates to the field of microbial preparations, and discloses a microbial preparation with crop transpiration resistance and application, the microbial preparation comprises the following components by weight: 20-30 parts of Piriformospora indica, 15-25 parts of Bifidobacterium, 25-35 parts of endophytic fungi, 8-12 parts of auxin, 3-7 parts of cytokinin, 3-7 parts of trace elements, and 2-8 parts of organic matter; according to the microbial preparation for resisting the transpiration of the traditional Chinese medicinal materials, provided by the invention, by combining the alternaria alternata, the piriformospora indica and the bifidobacterium, the stress resistance and the growth efficiency of the traditional Chinese medicinal materials are remarkably enhanced, and the transpiration resistance of the traditional Chinese medicinal materials is enhanced; the method has the advantages that the nutrient absorption and water utilization efficiency of the traditional Chinese medicinal materials is improved by improving a rhizosphere microbial ecological system, so that the optimal growth state is kept in drought or barren soil.

The invention discloses a Antarctic fish fin cell line and a construction method and application thereof, and the construction method comprises the following steps: step S1: collecting Antarctic fish fin tissues, cleaning and sterilizing, and cutting into pieces to obtain tissue blocks; s2, culturing the tissue blocks in a culture bottle to obtain primary culture cells; s3, when the primary culture cells grow to a certain degree in an adherent manner, trypsin-ED TA is added for digestion, and after the primary culture cells become round and fall off, a complete medium is added, so that the cells continue to grow; s4, when the primary culture cells grow to a certain degree, flushing the cells with PBS, adding trypsin-EDTA digestive juice for digestion, after digestion is completed, resuspending the cells, and carrying out subculture, so that the Antarctic fin cell line is constructed and completed. According to the technical scheme, the Antarctic fish fin cell line is good in growth state, stable in cell proliferation and capable of achieving continuous passage and can be used for expressing exogenous genes for gene function research.

This invention relates to the field of in vitro stem cell culture technology, specifically to a culture medium for the proliferation of muscle satellite cells, its culture method, and its application. The culture medium comprises a basal medium, fetal bovine serum, and plant-derived active substances; the plant-derived active substances include at least one selected from apigenin, resveratrol, and quercetin; the volume percentage of fetal bovine serum in the culture medium is 2%-5%. This invention, by adding plant-derived active substances, effectively maintains cell stemness and significantly promotes cell proliferation at low serum concentrations.

The invention relates to the technical field of cell models, and discloses a method for inducing osteoclast in vitro from mouse mononuclear macrophage leukemia cells, which comprises the following steps: S10: passage of mouse mononuclear macrophage leukemia cells: inoculating the mouse mononuclear macrophage leukemia cells into a DMEM (dulbecco's modified eagle medium) high-glucose complete medium containing special-grade fetal calf serum; performing passage; s20, inducing mouse mononuclear macrophage leukemia cells: collecting 1-5 generations of mouse mononuclear macrophage leukemia cells, inoculating the mouse mononuclear macrophage leukemia cells into a DMEM high-glucose complete medium containing special-grade fetal calf serum, and adding a nuclear factor kappa B receptor activating factor ligand into the medium after 20-24 hours; s30, continuously culturing for 40-48 hours, replacing the culture medium, and adding a nuclear factor kappa B receptor activating factor ligand at the same time; and S40, continuing to culture for 40-48 hours to obtain mature osteoclasts, so that the in-vitro osteoclast induction efficiency of RAW264.7 cells can be improved.

The invention belongs to the technical field of plant cultivation, and particularly relates to a Mongolian medicine plant Artemisia stelleriana cutting propagation method, which comprises the following steps: S1, disinfecting Artemisia stelleriana branches; s2, pruning the disinfected Artemisia stelleriana branches, and performing disinfection again to obtain Artemisia stelleriana cuttage segments; s3, treating the cutting sections of the Artemisia stelleriana by using a rooting agent, and cutting the cutting sections of the Artemisia stelleriana subjected to rooting treatment in a matrix for cutting propagation. The cutting propagation method of the Mongolian medicine plant Artemisia stelleriana shows remarkable beneficial effects in the aspects of ecological environment protection, propagation efficiency improvement, germplasm resource enrichment, stress resistance enhancement, economic benefit improvement and the like, and has important popularization and application values.

The invention discloses a construction method of a primary cell organoid model and application of the primary cell organoid model in drug screening, the construction method comprises the following steps: (1) centrifuging malignant pleuroperitoneal fluid samples derived from digestive tract tumor patients, collecting cell precipitates, and preparing a single-cell suspension; (2) performing primary culture on the single-cell suspension to obtain primary culture cells; and (3) mixing the primary culture cells with matrigel, and carrying out organoid culture to obtain the primary cell organoid model. By adopting the method provided by the invention, the homogeneity of the organoids cultured by using the primary cells is higher, the number of successfully cultured organoids is more, and meanwhile, the related problems of limited passage and organoid amplification culture library building in the field of organoid culture are solved.

This invention belongs to the field of plant tissue culture technology, specifically relating to a tissue culture rapid propagation medium and method for rooting *Carex latifolia*. The invention provides a tissue culture rapid propagation medium for rooting *Carex latifolia*, comprising a rooting medium; the rooting medium uses 1 / 2 MS as the basal medium and further includes NAA 0.8 mg·L⁻¹, IBA 1.0 mg·L⁻¹, sucrose 30 g·L⁻¹, and agar 7 g·L⁻¹, with a pH of 5.8-6.0. The invention also provides a method for direct rooting of *Carex latifolia* through tissue culture rapid propagation. Aseptic seedlings of *Carex latifolia* have their outer, weaker leaves removed, and the original root system cut off, retaining 2-3 cm of the above-ground portion. These seedlings are then inoculated into the rooting medium for rooting culture to obtain rooted seedlings. After hardening off the rooted aseptic seedlings, the well-rooted *Carex latifolia* seedlings are transplanted into a substrate to form new, complete plants. The method for rapid propagation of broadleaf sedge by tissue culture provided by this invention has the characteristics of high rooting rate, large average number of roots, well-developed root system, high transplant survival rate, and robust regenerated seedlings.

The invention discloses an intelligent garden irrigation route optimization method and system, and the method comprises the following steps: S1, collecting and preprocessing data, and generating an initial multi-dimensional environment feature vector; s2, inputting the initial multi-dimensional environment feature vector into an adaptive modal aggregation algorithm to generate an irrigation demand intensity index; s3, inputting the irrigation demand intensity index into the multi-scale spatial-temporal feature fusion model to generate an irrigation demand change curve; s4, performing irrigation path planning in combination with an adaptive evolutionary algorithm and upper bound confidence interval tree search, and generating a preliminary irrigation path; s5, transmitting the preliminary irrigation path to a dynamic obstacle detection module driven by semantic segmentation, and dynamically adjusting the irrigation path; s6, the working sequence of irrigation equipment is arranged, and irrigation operation is executed; and S7, inputting the feedback data into a causal reasoning module, and extracting real influence characteristics of irrigation on plant health. According to the method, the intelligent dynamic adjustment of the garden irrigation path is realized by combining methods such as multi-modal perception and upper bound confidence interval tree search.