471 results about "Microplastics" patented technology

The invention discloses a composite fiber woven net active microbial carrier for treating nitrogen-containing wastewater as well as a preparation method and application of the composite fiber woven net active microbial carrier. The active microbial carrier is formed by weaving titanium wires, rock wool wires, iron-carbon twisted wires and PLA wires, has high biocompatibility and high specific surface area, keeps high mechanical strength, can promote efficient biofilm formation of denitrification sludge, and can promote quick start of a reactor and improve the denitrification effect after successful biofilm formation. According to the application, the requirements of'plastic reduction and plastic forbidding 'are met, the traditional plastic biological filler is replaced, the technical problem that micro-plastic particles are released due to degradation in the use process of the filler is solved, the requirement of green development is met, and remarkable economic and ecological environment values are achieved. Iron wires and carbon fibers in the iron-carbon stranded wires form a primary battery in the reactor, controllable dissolution of iron ions is spontaneously accelerated, driving is not dependent on external potential, and the concept of low carbon and environmental protection is met; additional carbon source adding is reduced through the PLA filaments, and the PLA filaments are further matched with the low-carbon and environment-friendly concept.

The invention discloses a paper base-PHA double-sided composite soil biodegradable agricultural mulching film as well as a preparation method and application thereof, and belongs to the technical field of agricultural mulching materials and green high polymer materials. The mulching film comprises a paper base layer and a homologous polyhydroxyalkanoate covering layer arranged on the surface of the paper base layer. Through double-sided synergy of the water-based PHA coating on the soil sticking surface and the thermoplastic PHA extrusion film layer on the windward and rainy surface, and in combination with a'wet-to-heat 'integrated composite process and trace anhydride grafting, super-strong combination of a paper-plastic interface fiber tearing level is realized, and the problem that traditional paper-plastic is easy to layer is solved. Compared with a traditional fluorine-containing product, on the premise that total organic fluorine is not detected, the excellent barrier and moisture conservation performance is achieved with the thinner coating thickness by means of double barriers. The product has excellent mechanical laying adaptability and long-season weather resistance, is fully biodegradable, thoroughly solves the hidden dangers of micro-plastic residues and persistent organic pollutants, and is an ideal green agricultural covering material.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

The invention discloses a machine vision-based micro-plastic intelligent identification and measurement method, which comprises the following steps: filtering a water sample containing micro-plastic or not containing micro-plastic, and acquiring images under a uniform amplification factor by using a microscope to obtain a micro-plastic image to be detected and a contrast image; calculating the absolute value of the color centroid value of the contrast image and the color difference value of the micro-plastic image, generating a difference image, and carrying out denoising, graying and binarization processing on the difference image to obtain a binary image with highlighted micro-plastic; extracting pixel coordinates of the micro-plastics, setting a threshold value to carry out connected clustering, and distinguishing the micro-plastics and image impurities in combination with a preset micro-plastic recognition threshold value to realize micro-plastic recognition and quantity statistics; and further shooting a standard objective lens micrometer image, calculating the actual physical size represented by a single pixel, and automatically calculating the area and the length of the micro-plastic by combining each clustering enclosing rectangle and the number of the pixels. The method is accurate in recognition and high in operation automation degree, and can be widely applied to intelligent recognition and analysis of water body micro-plastics.

The invention relates to the technical field of monitoring analysis, and discloses a microplastic degradation process monitoring system based on microbiological treatment, which comprises the following modules: a microbiological culture module for culturing microorganisms in a microplastic degradation process and adjusting a plurality of parameters through an environmental reactor to maintain the activity of the microorganisms; the data monitoring and processing module is used for monitoring and acquiring a plurality of key indexes of the micro-plastics in the degradation liquid in the degradation process through a plurality of sensors, removing and supplementing abnormal data, and finally synchronously uploading non-abnormal data to the cloud through the Internet of Things node; and the intelligent analysis platform comprises a degradation prediction unit and a reporting unit. Through one-way comparison and relevance comparison, abnormal data monitored by the sensor are removed, the microplastic degradation efficiency is predicted by using the living environment of microorganisms, the concentration of the microorganisms and a degradation kinetic model, and the microplastic degradation efficiency can be comprehensively analyzed in combination with multiple parameters during microplastic degradation analysis.

The invention discloses a micro-plastic type and aging degree combined identification model training and application method and a related device, and relates to the technical field of micro-plastic identification, and the method comprises the steps: obtaining a sample hyperspectral image and a sample label of each micro-plastic sample in a plurality of micro-plastic samples, the sample label comprises a micro-plastic type and a micro-plastic aging degree, extracting a sample spectrum feature vector of each sample pixel point in the sample hyperspectral image, setting the sample label corresponding to the sample hyperspectral image as the sample label of each sample pixel point in the sample hyperspectral image, constructing a data set, and extracting the sample spectrum feature vector of each sample pixel point in the sample hyperspectral image; the data set comprises a sample spectrum feature vector and a sample label of each sample pixel point, constructing a deep learning model, training the deep learning model by using the data set, and obtaining a micro-plastic type and aging degree combined identification model. And the identification efficiency and accuracy are improved.

The invention relates to the technical field of analysis and detection, in particular to a method for simulating and detecting micro / nano plastic released by food contact plastic products. The method provided by the invention aims at the blank of the existing food-source micro-plastic detection system, simulative component liquid is collected by simulating the contact between food components and food plastic products, micro-nano plastics are separated through a 1m filter membrane, and micro-sized plastics on the filter membrane are qualitatively and quantitatively detected by adopting a microscopic infrared spectrum and a scanning electron microscope. The water sample under the filter membrane is subjected to qualitative and quantitative treatment on the nano-plastic by a thermal cracking-gas chromatography-mass spectrometry combined technology and a nano-particle tracking analyzer, so that programmed comprehensive detection is realized. According to the method, the amount and what kind of micro-nano plastic can be released by the plastic product in a real use chain are more clearly revealed, support is provided for a market supervision system, health and life safety of the masses are guaranteed, and the method has a good application prospect.

The invention discloses a micro-plastic detection model construction method, a detection method, equipment and a medium. The method comprises the following steps: constructing a micro-plastic detection model; the feature extraction module is used for extracting feature maps of various different scales from an input image, performing feature fusion and feature enhancement on the feature maps of various different scales, and then identifying micro-plastic information from the feature maps and outputting the micro-plastic information; the model comprises an MFM module, an FS-C3k2 module and a GSConvE module; the MFM module is used for carrying out feature fusion on feature maps of different scales; the FS-C3k2 module is used for dividing an input feature map into two channels, one channel is spliced with the other channel after space-frequency feature extraction and feature enhancement, and a first enhanced feature map is obtained; the GSConvE module is used for performing deep feature enhancement on the first enhanced feature map; and taking model loss function minimization as a target training model. According to the invention, the precision and accuracy of micro-plastic detection are improved.

The invention provides an aerogel film with a continuous gradient pore structure and a preparation method and application thereof.The preparation method comprises the steps that a pre-crosslinking solution containing a polymer nanofiber suspension, a sodium alginate solution, a polyethyleneimine aqueous solution and a crosslinking agent is sprayed to a temperature-conducting substrate with the initial temperature ranging from-20 DEG C to-15 DEG C at high pressure; and freeze-drying to obtain the aerogel film with the continuous gradient pore structure. The aperture of the aerogel film is gradually increased from bottom to top, and the range of the aperture is mainly 1-110 [mu] m. The aerogel film is placed in a filter membrane clamp, so that a solution to be filtered passes through the aerogel film according to the sequence of'macropore-mesopore-micropore ', the aerogel film is used for intercepting and recycling particles with different sizes, such as micro-plastics, and high water flux and high rejection rate are both considered. The pore size of the aerogel film can be changed by adjusting the temperature of the temperature-conducting substrate; the prepared aerogel film improves the trade-off effect in the existing filtration separation technology, and has a wide market prospect.

A method for detecting the extent of microplastic accumulation in bivalve organisms in extreme deep-sea environments is provided. This method is performed through sampling of biological communities to represent the community structure of that biological bed layer, then morphological characterization statistics are conducted to classify the age stage data of individual bivalves, multivariate factor analysis is used to obtain the individual bivalves with the greatest degree of contribution in each classified age stage, subsequently tissue-specific microplastic extraction is performed, the morphology of seafloor microplastics is restored and streamlined identification of full-size microplastics is considered, carbon-14 dating is used to trace the duration of microplastic adsorption by each individual bivalve, dynamic accumulation curves for seafloor bivalves are constructed by connecting each bivalve's survival duration, and finally this is scaled up to the entire bivalve bed to derive the microplastic accumulation rate and historical accumulation of the entire extreme ecosystem.

The invention relates to the technical field of underwater micro-plastic collection, and discloses a self-cleaning low-energy-consumption ocean micro-plastic multi-stage collecting device which comprises a floating energy assembly, a coarse filtering assembly, a first micro-plastic filtering and collecting assembly and a second micro-plastic filtering and collecting assembly which are sequentially arranged from top to bottom and fixedly connected. The floating energy assembly comprises a hollow floating table, a solar power generation panel, a hollow connecting pipe, a central control assembly and a driving motor; the driving motor is located in the hollow connecting pipe and connected with the rotary filtering roller through a bolt; the coarse filtering assembly comprises a protective cover shell, the rotary filtering roller and a flow guide device; the first micro-plastic filtering and collecting assembly comprises a micro-plastic cleaning and recycling bin and a plurality of layers of electrostatic adsorption carbon fiber nets; the second micro-plastic filtering and collecting assembly comprises a micro-plastic recycling bin, a micro-plastic capturing assembly and a hydraulic driving assembly. Through the design of the upper-layer filter module, the middle-layer filter module and the lower-layer filter module, accurate interception of micro-plastics in different particle size ranges is achieved.

Systems and methods are provided for comprehensive in vivo nanotoxicity testing, as well as fabrication methods of chips used for nanotoxicity testing. A three-dimensional (3D)-printed, automated microfluidic chip can be configured for in vivo nanotoxicity testing, and the chip can allow for multi-route (e.g., waterborne and / or foodborne) exposure of marine organisms to small materials (e.g., plastics such as microplastics and / or nanoplastics (NPLs), or metals), providing real-time, in situ monitoring of bioaccumulation and oxygen consumption.

The invention belongs to the technical field of micro-plastic image detection and processing, and particularly relates to a micro-plastic feature extraction and shape recognition method based on multi-scale morphology. According to the specific technical scheme, the method comprises the following steps: preprocessing a micro-plastic sample image, constructing a structural element group covering a multi-scale range, and extracting multi-scale edge features of micro-plastic by using a morphological multi-scale iterative filter and a multi-scale edge detection algorithm; carrying out weighted fusion on the extracted multi-scale edge features through a multi-scale edge fusion algorithm, and extracting morphological feature parameters of the microplastics; and constructing and training a classification model by taking the morphological characteristic parameters as input and the micro-plastic shape categories as output. The problems that in the prior art, micro-plastics with various sizes and complex shapes are difficult to recognize, and manual recognition is relied on are solved, the method is suitable for large-scale detection of complex environment samples, and efficient and reliable technical support is provided for micro-plastic pollution monitoring.

The invention relates to the technical field of fluid dynamics experimental measurement, and discloses a water tank experimental device and method for muddy current transportation of micro-plastics, the device comprises a water tank device main body, an optical measurement subsystem and an ultrasonic Doppler flow velocity measurement system, an ultrasonic emission path and an optical measurement window are overlapped in space, and the optical measurement subsystem and the ultrasonic Doppler flow velocity measurement system are arranged in the water tank device main body. The method comprises the following steps: performing first fluorescence labeling and second fluorescence labeling on the micro-plastic particles and the sediment particles; synchronously acquiring optical data and acoustic data in the cross window; respectively establishing a concentration correction function and a flow velocity correction function by taking acoustic backscattering intensity data and flow velocity data as references in the cross window; applying the correction function to the whole optical window to obtain a globally corrected concentration field and a globally corrected flow velocity field; and distinguishing and quantifying the micro-plastics and the sediments based on the corrected data. Through the cross correction principle, the problem of signal distortion of optical measurement in high-turbidity fluid is solved.

The invention relates to the technical field of wastewater treatment, in particular to a micro-plastic removal and recovery method and system based on magnetic adsorption-dissociation. The method comprises the following steps: S1, magnetic adsorption agglomeration; S2, magnetic separation; and S3, dissociation and recovery. The system comprises a raw water tank, a magnetic seed adding unit internally provided with a magnetic seed storage tank, a mixing reactor, a magnetic separator, a dissociation regeneration tank and a secondary magnetic separator which are connected in sequence, the method disclosed by the invention has high-efficiency removal capability on micro-plastics under different water quality conditions, regeneration cycle performance of magnetic seeds and adaptability and stability to complex water bodies, and the technology has remarkable advantages in the aspects of deep removal of the micro-plastics and resource recovery.

The invention relates to the technical field of micro-plastic collection, in particular to surface environment micro-plastic synchronous collection and separation integrated equipment and a synchronous collection and separation method. The equipment comprises a gas collecting device, a circulating gas pump, a circulating water pump, a cooling ring and a flotation tower, a cooling ring is arranged on the upper portion of the flotation tower, a corundum aeration device is arranged at the bottom of the flotation tower, one end of a circulating air pump is connected with two sets of air inlets of the air collecting device through pipelines, and the other end of the circulating air pump is connected with an outlet in the top of the flotation tower through a pipeline. When the equipment works, the operation of the equipment needs to be ensured in a closed circulation process. The equipment can collect micro-plastics with various particle sizes in the surface environment and other impurities capable of diffusing through natural conditions, synchronously enrich the micro-plastics and separate the impurities, so that the purpose of efficiently collecting micro-plastic samples is achieved.

The invention discloses a confocal laser microscope detection method for micro-plastic surface biofilm components and biomass, and belongs to the field of micro-plastic pollution monitoring. The method comprises the following steps: carrying out stratified sampling and polymer component identification on micro-plastic in an environmental water body; the method comprises the following steps: marking algae, blue-green algae, bacteria and extracellular polymeric substances in a biological membrane through specific fluorescent staining, carrying out high-resolution three-dimensional imaging by utilizing a confocal laser microscope, and realizing batch quantitative analysis of each component of the biological membrane by adopting ImageJ software. The technical problems that an existing detection method is insufficient in parameter dimension, evaluation result deviation is caused only based on partial component quantification, and the influence of the overall structure of the biological membrane on the micro-plastic migration behavior cannot be truly reflected are solved. According to the method, the biomass and spatial distribution of each component of the biological membrane can be comprehensively and accurately quantified, the accuracy of analyzing the vertical migration mechanism of the micro-plastic is improved, and multi-dimensional data support is provided for ecological risk assessment.

The invention belongs to the technical field of micro-plastic detection, and particularly relates to a method for separating and detecting micro-plastic in a sample. The separation method comprises the following steps: cracking a sample, and then separating by using gas chromatography; the cracking temperature is 500-600 DEG C, and the gas chromatography heating procedure comprises the following steps: keeping the initial temperature of 35-45 DEG C for 1-2 minutes, heating to 310-330 DEG C at 25-35 DEG C / min, and keeping the temperature for 1-15 minutes. According to the separation method, eight micro-plastics (PS, PVC, NY-6, PP, ABS, PC, PU and PMMA) can be separated out at the same time within 10 min, the separation efficiency is high, and subsequent qualitative and / or quantitative analysis of the micro-plastics is facilitated.

The application relates to the technical field of wastewater treatment, and particularly discloses a degradation method and application of microplastics in aquaculture wastewater. The degradation method comprises the steps of preparation of a mesoporous structure magnetic immobilized material, pretreatment, adsorption-enzymolysis, microbial degradation, magnetic separation, precipitation and the like. The shell layer and the mesoporous structure of the mesoporous material are used to efficiently immobilize composite enzymes, so that enzyme loss is avoided, and the composite enzymes are used for targeted degradation of PET, PE and PP, and the total microplastic removal rate is more than 94.5%. The composite heat-resistant bacteria relay enzymolysis product is deepened and mineralized, and a phased treatment agent is matched to enhance mass transfer, so that the microbial activity is increased by 3 times. The mesoporous material is protected by the shell layer, the enzyme activity retention rate is more than 85%, the effluent COD is less than or equal to 50 mg / L, meets the discharge requirements, is suitable for microplastic pollution treatment in multiple scenes, and has the advantages of high efficiency, economy and environmental friendliness.

The invention relates to a microplastic visualization method and device, electronic equipment, a storage medium and a program product. The method comprises the following steps: obtaining a Raman spectrum of a micro-plastic sample; based on the Raman spectrum, determining the peak top and the peak bottom of the characteristic peak of the micro-plastic in the micro-plastic sample; and based on difference information between the peak top and the peak bottom of the characteristic peak of the micro-plastic, performing chemical imaging extraction on the Raman spectrum to generate a chemical imaging image of the micro-plastic sample.

The application discloses a composite degradation preparation for degrading microplastics in wastewater and an application method thereof, and relates to the technical field of wastewater purification. The preparation is prepared by three steps of preparing a rare earth doped titanium-based porous framework, preparing an enzyme-containing bacteria coated precursor solution, and in-situ cross-linking and co-fixing. The framework realizes visible light response and magnetic separation characteristics. The co-fixing of enzyme and bacteria is completed by microcapsule embedding and carrier adsorption cross-linking. The preparation integrates the functions of photocatalysis, enzymatic degradation and microbial degradation. In application, the wastewater is pretreated to SS≤60mg / L, and then the preparation is added to a degradation tank. Under the conditions of 395-450nm light, 25-40 DEG C, and pH 6.0-8.0, the wastewater is degraded by stage light irradiation. Finally, the preparation is recycled by magnetic separation. The preparation solves the problem of free radical damage in the light-biological synergy, and has strong impact resistance, high microplastic removal rate, and recyclable preparation. The preparation is suitable for continuous treatment of wastewater, greatly reduces the operation cost, and is suitable for purification treatment of various wastewater containing microplastics.

The invention relates to the technical field of plant conditioners, in particular to application of nano silicon dioxide in relieving stress of polystyrene microplastics on hibiscus mutabilis. The invention finds that the influence of polystyrene micro-plastic on the stress of hibiscus mutabilis can be relieved to a certain extent during treatment by nano-silica, the intervention of the nano-silica causes the increase of secretion of melatonin, and the combined action of the nano-silica and melatonin makes the effect more remarkable and shows a synergistic effect. The nano silicon dioxide directly acts on the surfaces of cells to form a protective layer, so that adsorption and damage of PS-MPs to the cells are reduced, synthesis of melatonin is promoted, and a melatonin signal channel is activated. The activated melatonin signaling pathway further regulates the activity of carbon and nitrogen metabolism key enzymes, promotes carbon and nitrogen metabolism, improves synthesis and accumulation of carbohydrates and proteins, enhances the activity of an antioxidant system, removes active oxygen and reduces oxidative damage.

The application discloses a detection technology based on micro-plastic adsorption of a micro-fluidic chip, which forms a detection area by fixing micro-plastic particles in a chip channel in situ through a sodium alginate and calcium chloride system, and deducts background interference synchronously by using a parallel reference channel, and then pumps a solution containing a target adsorbate into the chip at a controllable flow rate, and simultaneously, real-time in-situ optical monitoring of the adsorption process is carried out by using a fluorescence technology, finally, the adsorption kinetics parameters and the adsorption isotherm are quantitatively calculated by analyzing the signal and time curve and the concentration gradient experiment, and the determination of the maximum adsorption capacity is realized; the micro-plastic is fixed, dynamic adsorption, real-time monitoring and data processing are integrated on the micro-fluidic chip platform, the problems that the traditional method cannot realize real-time observation, the background interference is large and the operation is complicated are effectively solved, and efficient quantitative analysis of the adsorption behavior of the micro-plastic is realized.

The invention discloses a soil micro-plastic microscopic Fourier infrared spectrum rapid detection method, and relates to the technical field of environmental pollutant analysis and detection.The method comprises the steps that a soil sample of a target area is collected; performing micro-plastic separation and extraction on the soil sample to obtain a micro-plastic sample; performing vacuum filtration on the micro-plastic sample and transferring the micro-plastic sample to a to-be-detected filter membrane; performing Fourier infrared microscopic scanning on the filter membrane to be detected to obtain a Mosaic image; carrying out particle point positioning on the Mosaic image by adopting a Fourier infrared detection method, and collecting spectral information of each particle point; the spectrum information of each particle point is matched with a micro-plastic standard spectrum library, micro-plastic type information of each particle point is determined, and a detection report is generated and comprises the point position matching rate, the area ratio, the particle length, the particle width and the content of different types of micro-plastics. According to the invention, rapid and accurate detection of the soil micro-plastic can be realized, and the detection precision and efficiency of the soil micro-plastic can be improved.

The invention provides a micro-plastic filling permeation immersion type interface coupling reaction experiment device which comprises a rectangular frame, a reaction column cylinder, a T-shaped strip and an iron convex block, a piston rod is movably arranged in the reaction column cylinder, a material guide pipe is arranged at the upper end of the right side of the reaction column cylinder, and a micro plug valve is arranged at a liquid inlet in the lower end of the reaction column cylinder. Guide grooves are formed in the left side and the right side of the interior of the rectangular frame, a magnetic block is installed on the upper portion of the interior of the rectangular frame, a U-shaped iron frame is fixed to the upper end of the right side of an iron protruding block, and a positioning rod is installed at the right end of the lower portion of the U-shaped iron frame in a penetrating and sliding mode. And the reaction solution uniformly permeates through the filling layer by utilizing the negative pressure of the driving core, so that the full-domain immersion type full contact between the micro plastic particles and the reaction solution is realized.

The utility model provides a water purifier for removing micro-plastics in drinking water, which belongs to the technical field of drinking water treatment, and comprises a fiber filter unit, a ceramic filter unit, a graphene adsorption unit and a support which are sequentially connected through pipelines, the water purifier can effectively remove the micro-plastics, the removal rate reaches more than 98%, and the service life of the water purifier is prolonged. Filtration and adsorption are combined, micro-plastics with different particle sizes are removed step by step, the running efficiency of equipment is improved by means of the pressure of tap water, and the device is suitable for household terminal water purification.

This invention provides a microplastic particle screening and separation device and method based on photoinduced dielectrophoresis. The device comprises a photoinduced dielectrophoresis chip consisting of a first illumination region, a second illumination region, a controllable barrier block, and a microchannel layer. By applying light to different positions on the photoinduced dielectrophoresis chip, the conductivity at the illumination position changes, thereby generating a non-uniform electric field. Due to the different radii of the microplastic particles, the magnitude of the photoinduced dielectrophoretic force varies. Larger-radius microplastic particles experience a greater dielectrophoretic force and cannot pass through the illumination region, while smaller-radius microplastic particles experience a smaller dielectrophoretic force and can flow to the sample outlet. By changing the position of the controllable barrier block, the separation of microplastic particles of three different radii can be achieved. The microplastic particle screening and separation device and method based on photoinduced dielectrophoresis described in this invention can achieve the separation and screening of target microplastics of different sizes using contactless photoinduced dielectrophoresis force.

The application discloses a kind of microscale microplastic migration occurrence multi-physical field coupling simulation method, belong to ecological environment field.The method aims at solving the problem that existing microplastic migration model ignores interface adsorption process, is difficult to couple the action of multiple physical fields and is not suitable for the problem of insufficient adaptability to heterogeneous environment.Its main points are: in the Lagrangian framework, by constructing seepage field, and comprehensively calculating the stokes drag, dielectrophoresis force and Brownian force suffered by microplastic particles to track its movement, while explicitly introducing the adsorption and desorption between particles and pore wall, to simulate the migration trajectory and occurrence state of microplastic in heterogeneous porous medium.The application provides a high-precision simulation tool for revealing the environmental fate and health risk of microplastics in groundwater systems.

The present disclosure relates generally to a method for identifying at least one microplastic in a protein-containing liquid biological sample, wherein the method comprises: heating the protein-containing liquid biological sample to provide a protein-containing sediment and a liquid phase disposed above the sediment; separating the liquid phase from the protein-containing sediment; evaporating the liquid phase to provide an analyte; treating the analyte with a depolymerization solution comprising an organic phase and an aqueous phase; extracting the analyte from the depolymerization solution in the organic phase of the depolymerization solution; filtering the organic phase to provide an analyte residue as the oversize; and identifying the at least one microplastic in the analyte residue with size-exclusion liquid chromatography and mass spectrometry.