53 results about "Bacteroidales" patented technology

Methods and compositions are provided for treating weight related conditions and metabolic disorders by altering microbiota in a subject. One aspect provides methods and compositions to alter microbiota in a subject by administering to the subject a composition that includes a substantially purified microbiota from phyla such as Bacteroidetes, Proteobacteria, Firmicutes and Verrucomicrobia or orders such as Bacteroidales, Verrucomicrobiales, Clostridiales and Enterobacteriales or genera such as Alistipes, Clostridium, Escherichia, and Akkermansia. Another aspect includes a pharmaceutical composition for altering microbiota that includes a therapeutically effective amount of substantially purified microbiota and a pharmaceutically acceptable carrier. Yet another aspect includes methods for treating a disorder, such as obesity, in a subject in need of such treatment by changing relative abundance of microbiota in a gastrointestinal tract of the subject without or in addition to a surgical procedure.

Herein are described 1058 different bacterial taxa that were unique to either human, grazing mammal, or bird fecal wastes. These identified taxa can serve as specific identifier taxa for these sources in environmental waters. Two field tests in marine waters demonstrate the capacity of phylogenetic microarray analysis to track multiple sources with one test.

The invention discloses a kit for predicting adolescent obesity in childhood and an application thereof. The invention provides an application of a substance used for detecting the abundance of target bacteria in intestinal flora of children to be detected in preparation of products for predicting simple obesity of children, and the target bacteria are any one of Firmicutes, Clostridia, Clostridiales, Clostridiaceae, Clostridium, Bacteroidetes, Bacteroidia, Bacteroidales, Bacteroidaceae, Bacteroides, Oscillospira, Coriobacteriaceae and Collinsella. Specific information is obtained from a simple obesity queue of children to better embody the intestinal flora features of obesity of children, and by monitoring the abundance change of the characteristic species, the development and change of the obesity state can be predicated; the obtained intestinal flora features of obesity of children are compared with the intestinal flora features obtained by researching other diseases to further predict the risk and trend of related obesity diseases.

The present invention relates to belongs to the field of bacteria, and is especially one avirulent type-II pig streptococcus strain and its preparation process and application in developing pig streptococcicosis vaccine. The avirulent type-II pig streptococcus strain is Streptococcus suis (Serotype 2)05JYS68 in the preservation number of CGMCC No. 1902. The preparation process includes the following steps: sampling from pharynx and nose of health pig, mixing with THB culture medium, static culture at 30-39 deg.c for 3-6 hr, extracting total DNA as template, PCR, plate streak, Gram staining and blood serum agglutination experiment to determine the strain.

It provides a method for predicting the risk of an adverse pregnancy or neonatal outcome for a pregnant subject by detecting the elevated level of bacteria from one or more selected bacterial taxa (e.g., genera or species). A kit useful for such a method is also provided. In addition, it provides a method for determining the risk of having advanced cervical dilation and / or premature cervical shortening based on differentially abundant bacterial taxa.

The invention provides a method for detecting influence of microplastics and antibiotics on a sediment microbial community structure and an ecological function. The method comprises the following steps: acquiring a sediment sample at a sampling point; adding the micro-plastic particles and the antibiotic solution into the sediment sample, and incubating; dNA of the microorganisms is extracted, and 16S rRNA sequencing is carried out; and on the basis of a sequencing result, microbial community diversity and characteristic bacterial class group identification and ecological function evaluation are carried out. According to the invention, the comprehensive effect of micro-plastic and antibiotic combined pollution on the structure and ecological function of the microbial community in the sediment is comprehensively analyzed for the first time, and the detection speed and accuracy of the microbial community in the sediment are improved by applying a 16S rRNA detection technology; and a solid support is provided for formulating an environment protection strategy, optimizing pollution control and implementing an ecological restoration project.

The invention discloses a compound capable of effectively inhibiting or killing bacterial microorganisms. The compound has a structural general formula shown as the formula I (please see the formula in the description). Pharmacodynamic experiments prove that the compound can effectively inhibit or kill the broad-spectrum and multi-drug resistant staphylococcus aureus, streptococcus pneumoniae and staphylococcus aureus and is expected to be developed into a novel effective antibacterial drug with new targets.

The invention provides a bacterial strain and a flocculating agent for bacterial fermentative solution pretreatment as well as preparation methods and application methods of the bacterial strain and the flocculating agent. The flocculating agent is prepared by fermenting, culturing and extracting a microbial bacterial strain Pannonibacter phragmitetus B7 (CCTCC NO: M2013025), and then is used for bacterial fermentative solution pretreatment. The fermentation process of the microbial flocculating agent is simple; the flocculating agent is mainly secreted outside cells; and the extraction and preparation method of the flocculating agent is simple, low in cost and suitable for mass production. The acting pH value of the microbial flocculating agent is 2-13; and the flocculating agent is suitable for the flocculating and settling pretreatment of the bacterial fermentative solution and has an enormous popularization and application value.

The invention relates to a hyperspectral bacteria classification method based on lightweight deep learning and membership width learning, and belongs to the technical field of hyperspectral image processing and microbiological detection. The method comprises the following steps: collecting hyperspectral image data of food-borne pathogenic bacteria, and carrying out correction and region-of-interest extraction to obtain bacterial spectral data; the method comprises the following steps: preprocessing bacterial spectral data by using a fractional differential method, enhancing spectral features and suppressing noise to obtain enhanced spectral data, and inputting the enhanced spectral data into a lightweight deep network to extract multilevel space-spectral features to obtain depth features; inputting the depth features into a membership width learning module, and outputting a bacterial category classification result; wherein the membership width learning module comprises operations of defining a membership function, constructing a fuzzy score membership matrix and optimizing a width learning weight matrix. The objective of the invention is to solve the technical problems of insufficient feature extraction, outlier interference and high model complexity in hyperspectral bacterial classification in the prior art.

The invention discloses a method for evaluating apoptosis decline of bacteria by eversion staining of phosphatidylserine on a cell membrane. The method comprises the following steps: centrifuging, re-suspending, standing and centrifuging a liquid culture medium containing bacteria to be detected to obtain immobilized bacteria; adding a sterile phosphate buffer solution, and performing re-suspending to obtain a bacterial suspension; adding the lysozyme solution into the bacterial suspension, dissolving the wall, and performing centrifuging to obtain bacteria after dissolving the wall; uniformlymixing the bacteria subjected to wall dissolving with a sterile phosphate buffer solution to obtain a bacteria diluent; adding an AVF dye and the staining binding solution into the bacterial diluent,and performing staining; and detecting the fluorescence intensity by using a flow cytometer to obtain a red fluorescence intensity value and a green fluorescence intensity value. The bacterial cell staining method is simple to operate and obvious in staining effect, and is a method for improving staining obstruction removal, staining false positive elimination and the like of the conventional AVFstaining method for eukaryotic cell apoptosis staining for the first time and enabling the method to be suitable for evaluating bacterial cell apoptosis decline.

The invention relates to a primer group, a probe group and a kit containing the primer group and the probe group, which are used for synchronously detecting various common digestive tract infection related bacteria of children and can be used for simultaneously detecting various common bacteria causing digestive system infection by one-time reaction. The kit is the most comprehensive children digestive tract bacterial infection detection method at present, is high in efficiency and specificity, and provides an auxiliary diagnosis basis for clinical examination. According to the technical scheme, the technical problem that in the prior art, diagnosis and research on bacterial pathogenic bacteria related to children digestive tract infection are still insufficient can be solved.

The present invention provides a compound of Formula (I), which can be used to prepare a composition for losing weight and improving gut microbiota composition. The invention also provides a composition comprising an effective amount of the compound, which has the ability to increase the abundance of Bacteroidales, thereby reducing the ratio of Firmicutes to Bacteroidetes, and can increase the abundance of beneficial gut bacteria and change the gut microbiota composition. The compound and composition of the invention can be applied in food, medicine, feed, nutritional supplements and other uses.

The invention provides a bacteria classification method based on Raman spectrum data and related equipment, and the method comprises the steps: obtaining original spectrum data of different types of bacteria and a plurality of bacteria category labels, and constructing a multi-stage tree-shaped classification structure which represents the category affiliation relationship among the plurality of bacteria category labels; inputting the original spectrum data and the multi-stage tree-shaped classification structure into a classification model, extracting and fusing spectrum features and category features based on the original spectrum data and the multi-stage tree-shaped classification structure, generating a plurality of category perception features, calculating the similarity between the plurality of category perception features and preset prototype features, and obtaining a classification result; predicting a bacterial category prediction probability corresponding to the original spectral data based on the similarity so as to determine a target bacterial category of the to-be-detected bacteria; according to the method, feature coding of category perception can be performed on the spectral data based on a hierarchical structure of bacterial categories, so that the classification accuracy of bacterial classification is improved, and the bacterial classification effect is effectively optimized.

The invention belongs to the technical field of molecular hybridization detection, and particularly relates to a hybrid buffer solution system and in situ hybridization method suitable for bacteria FISH (Fluorescence in situ hybridization) detection. The hybrid buffer solution comprises the following ingredients at a ratio: 15% deionized formamide, 2*SSC, 0.5M EDTA (Ethylene Diamine Tetraacetic Acid), 5*Denhardt and 50mM NaH2PO4. The hybrid buffer solution disclosed by the invention is especially suitable for bacteria FISH detection. The hybrid buffer solution has the advantages of high resultspecificity, low result background, convenience in use, low cost and the like when the hybrid buffer solution is used for carrying out FISH hybridization.

The invention discloses a kit for predicting adolescent obesity in childhood and an application thereof. The invention provides an application of a substance used for detecting the abundance of target bacteria in intestinal flora of children to be detected in preparation of products for predicting simple obesity of children, and the target bacteria are any one of Firmicutes, Clostridia, Clostridiales, Clostridiaceae, Clostridium, Bacteroidetes, Bacteroidia, Bacteroidales, Bacteroidaceae, Bacteroides, Oscillospira, Coriobacteriaceae and Collinsella. Specific information is obtained from a simple obesity queue of children to better embody the intestinal flora features of obesity of children, and by monitoring the abundance change of the characteristic species, the development and change of the obesity state can be predicated; the obtained intestinal flora features of obesity of children are compared with the intestinal flora features obtained by researching other diseases to further predict the risk and trend of related obesity diseases.

The invention discloses a similarity and intermiscibility principle for guiding microorganism compatibility, and a compatibility method for improving function and efficiency of exogenously added microorganisms. The similarity and intermiscibility compatibility method provided by the invention comprises the following steps: firstly, analyzing a community structure in a target environment, and screening out main dominant communities in the community structure; screening out bacterial groups with obviously increased relative abundance, namely competitive dominance, by combining with an analysis result of biological activation; and finally, with a 16SrDNA sequence as a reference, screening a plurality of strains which simultaneously have stronger functions and are similar to target indigenousdominant communities or activated competitive dominant communities in phylogenetic development from a strain resource library for compatibility, and then culturing, mixing and adding the microorganisms into a target environment. The method is of great significance to petroleum pollution treatment.

The invention relates to the technical field of bacterial detection, in particular to a primer composition for simultaneously detecting pathogenic bacteria genes and drug-resistant genes, a detection method and a detection device. The core of the invention lies in synchronous detection of two main bacteria causing sepsis, namely burkholderia pseudomallei and klebsiella pneumoniae, and key drug-resistant genes by using a loop-mediated isothermal amplification (LAMP) technology. Specifically, the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-17, and can be used for detecting a burkholderia pseudomallei recA pathogenic gene, a kfB pathogenic gene of klebsiella pneumoniae, a quinolone antibiotic drug-resistant gene qnrA and a carbapenem antibiotic drug-resistant gene OXA-48 in a targeted manner. The primer composition disclosed by the invention can be used for simultaneously detecting various pathogenic bacteria genes and drug-resistant genes causing sepsis, the detection process is convenient, rapid and accurate, the detection result can be obtained within 40 minutes, and the detection effect is better.

The invention discloses a similarity and intermiscibility principle for guiding microorganism compatibility, and a compatibility method for improving function and efficiency of exogenously added microorganisms. The similarity and intermiscibility compatibility method provided by the invention comprises the following steps: firstly, analyzing a community structure in a target environment, and screening out main dominant communities in the community structure; screening out bacterial groups with obviously increased relative abundance, namely competitive dominance, by combining with an analysis result of biological activation; and finally, with a 16SrDNA sequence as a reference, screening a plurality of strains which simultaneously have stronger functions and are similar to target indigenousdominant communities or activated competitive dominant communities in phylogenetic development from a strain resource library for compatibility, and then culturing, mixing and adding the microorganisms into a target environment. The method is of great significance to petroleum pollution treatment.

The invention discloses a method for screening a probiotic combination based on multiple pathogenic bacteria of prawn diseases. The method is characterized by comprising the following steps: respectively acquiring intestinal bacterial community composition and abundance information of both healthy prawns at different time nodes and ill prawns at corresponding time points, and removing prawn age indication strains; screening bacterial groups which are not planted in the intestinal tracts of the prawns in an initial healthy state, are linearly increased along with the deterioration of prawn diseases and have abundance significantly higher than the abundance of healthy prawns at corresponding time points, defining the bacterial groups as multi-pathogenic bacteria, and determining the varieties of the multi-pathogenic bacteria; screening out strains with delta B smaller than -0.5 and an ASCH-score greater than 1.5 as direct antagonistic strains of multi-pathogenic bacteria, and defining the strains as probiotics; and taking a ratio of the relative abundance of a single probiotic to the sum of the relative abundance of the probiotics as the individual number percentage of the corresponding probiotics, and finally determining the combination and proportions of the probiotics. The method has the advantages of higher accuracy and better stability.

The invention discloses a method for screening and isolating chlorine-resistant bacteria and a Gordonia terrae strain JN724 obtained therefrom. In the method, bacteria are enriched in a sample and then screened for chlorine resistance, Separation and purification of chlorine-resistant bacteria, verification of chlorine resistance and other steps, screening and isolation of chlorine-resistant bacteria in the water supply system, and then obtaining a strain of chlorine-resistant bacteria, which has 100 homology with multiple strains of Gordonia %. Through the screening and isolation of chlorine-resistant bacteria, the number of bacteria in the water body and the composition of bacterial groups can be more accurately grasped, providing a more accurate theoretical basis for the microbial safety assessment of urban water supply, and providing support for the improvement of disinfection processes in water plants, thereby Better ensure the biological safety of drinking water.

The invention discloses a Cu2O-BSO antibacterial nano particle as well as a preparation method and an application of the Cu2O-BSO antibacterial nano particle. The preparation method comprises the following steps: (1) preparing Cu2O nanoparticles by adopting a Cu (NO3) 2 reduction method; (2) loading the sulphosulfoximine butyrate coated with dopa on the Cu2O nano particles obtained in the step (1), wherein the sulphosulfoximine butyrate coated with dopa is added into the Cu2O nano particles; and (3) adding polyethylene glycol into the product obtained in the step (2), and incubating to obtain the Cu2O-BSO antibacterial nanoparticles. The nano particles can effectively release Cu2O and BSO, the Cu2O and the BSO synergistically generate the effect of inducing bacterial copper death and the effect of inhibiting quorum sensing, the formation of a biological membrane and the expression of virulence factors can be influenced, and the synergistic antibacterial effect of non-antibiotics is not easily resisted by drug-resistant bacteria, so that drug-resistant pathogens can be effectively inhibited, and the drug resistance of the drug-resistant bacteria can be effectively inhibited. The compound can be used for treating diseases caused by drug-resistant bacterial infection.

The present invention concerns a method for treating or preventing a Cutibacterium acnes bacteria-associated disease in a subject, said method comprising modulating, in particular reducing, in said subject, the ratio of the amount of C. acnes bacteria of a newly identified category called α-type to the amount of C. acnes bacteria of newly identified category called β-type, the α-type category efficiently defining acne-associated strains and the β-type defining non acne-associated strains.

Methods for assessing the microbiome of a canid are provided. The methods include, inter alia, detecting one or more bacterial taxa in a sample obtained from the canid, correlating the one or more bacterial taxa to a control data set, and determining the microbiome biological age.

The invention provides application of diaphorina citri ubiquitin-binding enzyme E2J2 gene in prevention and control of citrus huanglongbing, and belongs to the technical field of animal gene engineering. The invention provides the application of the diaphorina citri ubiquitin-binding enzyme E2J2 gene with a nucleotide sequence as shown in SEQ ID NO.1 in prevention and control of the citrus huanglongbing. A specific primer is designed to synthesize dsRNA, the silent E2J2 gene is interfered by RNA, the gene silencing efficiency of the gene is detected, the gene is subsequently placed on a branch infected with Candidatus Liberibacter asiaticum for feeding, and the influence of the gene on Candidatus Liberibacter asiaticum (CLas) infection is studied. By blocking the transmission path of the liberobacter asiaticum in the diaphorina citri instead of controlling the diaphorina citri, a new strategy is provided for effective prevention and control of the liberobacter asiaticum, and a new prevention and control thought is provided for controlling transmission of bacterial pathogens by means of vector insects.

The invention provides a compound with a structure shown as a formula (I). The compound can be used for preparing a composition for reducing weight and improving intestinal flora. The invention also provides a composition containing an effective amount of the compound, and the composition has the capability of improving the abundance of the bacteriaceae flora, so that the ratio of the bacteriaceae to the bacteriaceae is reduced, the abundance of the intestinal beneficial flora can be improved, and the composition of the intestinal flora can be changed. The compound and the composition provided by the invention can be applied to foods, medicines, feeds, nutritional supplements and the like.

An embodiment of the present application provides a method for analyzing functional bacteria in activated sludge. The method includes: preprocessing the collected activated sludge sample to be analyzed; using a nucleic acid fluorescent staining agent to stain the preprocessed activated sludge sample to obtain the activated sludge sample to be detected; after determining the analysis parameters of the flow cytometer, using the flow cytometer to detect the activated sludge sample to be detected to obtain a first detection result; according to the analysis result parameters of the predefined standard microspheres and the first detection result, determining the concentration and particle size of the functional bacteria in the activated sludge sample to be analyzed, and further determining the biomass of the functional bacteria, the biomass of the first type of bacterial group, and the biomass of the second type of bacterial group in the activated sludge sample to be analyzed; thereby determining the metabolic activity of the functional bacteria in the activated sludge sample to be analyzed. To improve the analysis efficiency and accuracy of the functional bacteria in activated sludge.

The present invention discloses a Cu2O-BSO antibacterial nanoparticle, a preparation method thereof and an application. The preparation method comprises the following steps: (1) preparing Cu2O nanoparticles by a Cu(NO3)2 reduction method; (2) loading buthionine sulfoximine wrapped with dopamine on the Cu2O nanoparticles obtained in step (1); (3) incubating with polyethylene glycol in the product obtained in step (2) to obtain the Cu2O-BSO antibacterial nanoparticle. The nanoparticles of the present invention can effectively release Cu2O and BSO, and the two synergistically produce an effect of inducing cuproptosis in bacteria and inhibiting quorum sensing, which can affect the formation of biofilms and the expression of virulence factors. This non-antibiotic synergistic antibacterial effect is not easily resisted by drug-resistant bacteria, so it can effectively inhibit drug-resistant pathogens and can be used to treat diseases caused by drug-resistant bacterial infections.

The invention discloses a photosynthetic bacterium Rhodobacter sphaeroides strain, bacterial agent and its preparation method and application. The photosynthetic bacterium Rhodobacter sphaeroides strain is the photosynthetic bacterium Rhodobacter sphaeroides A13, which is preserved in China Typical Collection Center, the deposit number is CCTCC No: M 2018605. The photosynthetic bacterium Rhodobacter sphaeroides strain of the present invention, photosynthetic bacterium Rhodobacter sphaeroides A13 culture process is simple, culture time is short, cost is low, and photosynthetic bacterium Rhodobacter sphaeroides strain can be prepared by simple, fast, low-cost operation method A biocontrol agent and a biocontrol fermentation liquid for the prevention and control of rice bacterial streak disease are obtained.