The invention relates to a preparation method of a bionic oil-water separation copper net; and the preparation method is enlightened by hydrophobic self-cleanness of natural plant leaf surfaces, and an electrochemical deposition method is adopted to prepare super-hydrophobic surfaces with bionic micro-nanoscale double-layer classification. The method comprises the following steps: firstly, diluted hydrochloric acid, acetone and distilled water are used to clean the copper net for pretreatment; then, the copper net is dipped in electroplating liquid for electrochemical deposition, so that the surface morphology features are changed, and a bionic micro-nanoscale double-layer classification structure is formed on the surface of the copper net; after the electric deposition is finished, the copper net with the bionic micro-nanoscale double-layer classification structure is dipped into modifying solution, so that the surface thereof is formed with a low-surface-energy film; and finally, the surface of the copper net has super-hydrophobic and super-oleophylic characteristics. The preparation method of the bionic oil-water separation copper net is simple in operation, controllable in experimental parameters and low in cost, and can be used for manufacturing oil-water separation materials; and the materials have the super-hydrophobic and super-oleophylic characteristics, excellent mechanical stability and the recycling property.