The invention discloses a breeding swine frozen semen unfreezing diluent, and a preparation method thereof, and belongs to the technical field of swine frozen semen unfreezing. The breeding swine frozen semen unfreezing diluent can be used for solving a problem that semen oxidation damage of swine frozen semen is easily caused in unfreezing diluting process. The breeding swine frozen semen unfreezing diluent is composed of D-glucose, fructose, potassium chloride, disodium ethylene diamine tetraacetate, sodium bicarbonate, trisodium citrate, polyvinyl alcohol, gentamycin, astragalus polysaccharide, and resveratrol. A preparation method comprises following steps: 1, D-glucose, fructose, potassium chloride, disodium ethylene diamine tetraacetate, sodium bicarbonate, trisodium citrate, and polyvinyl alcohol are prepared into an aqueous solution with double distilled water; 2, the aqueous solution is introduced into a water-bath kettle at 26 DEG C for balance treatment until full dissolvingis realized, and the pH value is stable; 3, a bacterial filter is adopted for filtering; 4, and gentamycin, astragalus polysaccharide, and resveratrol are added 30min before using. The breeding swinefrozen semen unfreezing diluent is capable of achieving oxidation resistance effectively, and reducing oxidation damage of violent stability change in frozen semen unfreezing process on semen.