The present invention provides for rapid and large scale evaluation of expression of, or function of,
nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of
nucleotide sequences. The first component includes delivery in either a single event a
library of different engineered vectors or a single engineered vector for a single target
nucleotide sequence comprising sequences the function of which is desired to be known in
plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to
plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast
cycling and / or miniature size
plant and the final step involves
mass scale analysis of T0 plants for various phenotypes and
plasmid rescue to identify the nucleotide sequence present in a particular
phenotype.