The invention discloses a 
Burkholderia sp. Strain ES-89 for preventing and treating fungal diseases of plants as well as a culture method and application. The culture method comprises the following steps: A, inoculating an ES-89 strain into a 
nutrient broth culture liquid for activation; B, inoculating the activated ES-89 into a triangular flask with the 
nutrient broth culture liquid, and performing shake cultivation; C, centrifuging 
fermentation broth obtained from shake cultivation under a certain condition, and filtering an upper layer of 
fermentation broth by a 
bacterial filter to obtain sterile supernate; D, washing the bacterium with 
sterile water to prepare a bacterial suspension, and storing the 
fermentation broth, the fermentation supernate and the bacterial suspension. The strain is preserved with the preservation number of CCTCC, CCTCC NO: M 2016665, and the strain ES-89 can be applied to preparation of medicines for treating or preventing 
plant pathogenic fungi. The strain has an inhibition function on 
coptis chinensis 
leaf spot diseases and the like, and can be applied to prevention and treatment on multiple 
plant diseases. The prevention and 
treatment effect of the strain on the 
coptis chinensis 
leaf spot diseases can be 88% or greater, the culture method is feasible, the operation is simple and convenient, the 
pollution in the production process is reduced, 
solvent poisoning is avoided, and the application prospect is relatively good.