123 results about "TCEP" patented technology

The invention discloses a preparation method of an electrochemical sensor capable of simultaneously detecting two acute leukemia markers. The method comprises the following steps: respectively standing a lysozyme report probe and a gamma-interferon report probe in a liquid containing trichloroethyl phosphate (TCEP), so as to open a disulfide bond and respectively form a double-chain structure together with a lysozyme aptamer and a gamma-interferon aptamer; preprocessing a gold electrode, and immersing the processed gold electrode into the pre-processed mixed liquid of lysozyme report probe-aptamer double chains and gamma-interferon report probe-aptamer double chains; standing at room temperature over the night, and then cleaning by using secondary distilled water and a cleanout fluid; immersing the electrode into the liquid containing MCH to seal the electrode, and then cleaning the secondary distilled water and the cleanout fluid; and taking the electrode processed by the above steps as a work electrode to be connected on a chemical work station together with a reference electrode and a counter electrode, so as to obtain the electrochemical sensor. The electrochemical sensor can be applied to simultaneous detection of two acute leukemia markers, namely lysozyme and gamma-interferon.

The invention discloses a preparation method of a DNA (Deoxyribonucleic Acid) targeting nano medicine-carrying molecule for a brain tumor. The preparation method comprises the steps that a DNA single strand is added to a Tris-MgCl solution and mixed, and reacts to form a DNA tetrahedron solution; the DNA tetrahedron solution and a targeting peptide solution are added to a mixed solution of a PBS (Phosphoric acid solution), a CuSO4 solution, a TCEP (Tris-(2-carboxyethyl)-phosphine) solution and a TBTA (Tert-Butyl 2,2,2-trichloroacetimidate) solution, and subjected to thermostatic reaction; a targeting DNA tetrahedron solution is obtained; a tumor medicine is added to the targeting DNA tetrahedron solution; constant temperature oscillation, centrifugation, and supernatant removal are conducted; and an obtained sediment is the DNA targeting nano medicine-carrying molecule. According to the preparation method, peptide molecules having specificity in the tumor are modified on DNA tetrahedrons via a point-and-click reaction, so that construction of a targeting DNA nano-carrier is realized; and the targeting DNA medicine-carrying molecule has extremely high specific recognition function, low cytotoxicity and good structural stability.

The invention discloses a cold-resistance flame-retardant anti-static polyvinyl chloride sheathing material for coal mine cable; the sheathing material is characterized by consisting of the following raw materials: polyvinyl chloride resin SG3, tri(2-chloroethyl) phosphate, an anti-static agent, dioctyl sebacate, dioctyl phthalate, tricresyl phosphate, antimonous oxide, zinc borate, tribasic lead sulfate, bibasic lead sulfate, light calcium carbonate, bisphenol A, 2-mercapto benzimidazole, vinyl tri(beta-methoxyl ethyoxyl) silane, accelerant TMTD and modified asbestos powder; the polyvinyl chloride sheathing cable material provided by the invention has the following characteristics of being low in surface resistance value and high in anti-static property; by adopting the compound high-concentration anti-static agent consisting of TCEP (trichloroethyl phosphate), quaternary ammonium salt cationic surface active agent and nonionic surface active agent, the excellent anti-static synergistic effect can be brought; the surface resistance value of the sheathing material is no higher than 108ohms, and thus the sheathing material has excellent anti-static property.

The invention provides a silane crosslinking halogen-free flame retardant polyvinyl chloride cable material. The cable material is prepared from the following raw materials in parts by weight: 70-80 parts of polyvinyl chloride resin, 10-20 parts of acrylic acid grafted modified acrylic resin, 1-2 parts of acetate butyl, 1-3 parts of ethyl trifluoroacetate, 8-10 parts of zinc borate, 2-3 parts of molybdeni trioxidum, 1-2 parts of copper oxide, 10-12 parts of magnesium hydrate, 8-10 parts of ammonium polyphosphate, 6-10 parts of triethyl citrate, 4-5 parts of fumarate, 10-15 parts of TCEP fire-retardant plasticizer tri-beta-chloroethyl phosphate, 1-2 parts of calcium-zinc stabilizer, 1-2 parts of calaium strarate, 0.2-0.3 part of zinc stearate, 8-10 parts of light calcium carbonate, 3-5 parts of talcum powder, 8-10 parts of calcined clay, 3-4 parts of magnesium carbonate, 0.2-0.3 part of glass bead, 1-2 parts of carbon alcohol ester double 18, 1-2 parts of polyethylene wax, 0.2-0.5 part of lead sulphate tribasic, 20-30 parts of heavy calcium carbonate, 3-5 parts of barium stearate, 0.5-0.8 part of lead stearate dibasic, 0.5-1.0 part of ethenyl triethoxysilane, and 8-10 parts of modified filler. The cable material has a good mechanical property, is good in heat resistance and insulating property, and has the characteristics of good ageing property, high flame resistance and environment protection property and the like.

The invention discloses a homogeneous phase multi-component immunoassay method based on a surface plasma coupling effect. A gold nanosphere probe (Raman reactive dyes and capture antibodies are embellished on the surface of the gold nanosphere probe) and a gold nanorod probe (Raman reactive dyes and detection antibodies are embellished on the surface of the gold nanorod probe) are respectively prepared; the Raman reactive dyes carry sulfydryl self and are free of fluorescent background; and the antibodies are subjected to TCEP (Tris(2-carboxyethyl) phosphine) reduction treatment. The antigen of an object to be tested has immune sandwich reaction with the antibodies so as to generate an immune complex, so that the distances between nano particles are shortened, the surface plasma coupling effect is generated, the Raman signals of the surface embellishing dyes on the surfaces of the nano particles are significantly enhanced, and the SERS (Surface Enhanced Raman Scattering) quantitative analysis of the antigen is realized by using the coupling enhancing effect. The method is simple and rapid, the detection process needs only one step of operation without repeated elution or separating samples, the synchronous quantitative analysis to a plurality of components in the samples is realized with high sensitivity and good uniqueness, and thus the method is expected to become a new method in proteomics research and drug therapeutic effect evaluation.

The invention relates to a new effective and lasting method for making wool fabric have the antibacterial and anti-static functions. According to the method, firstly Gemini quaternary ammonium salt IDMA is synthesized, the synthesized product is detected and analyzed through infrared spectroscopy, 1H-NMR analysis, mass spectrometry and element analysis, and it is determined that the product is a target product; then, tris(2-carboxyethyl) phosphine is used for pre-processing wool fabric, and disulfide bonds in keratin of wool fibers are ruptured so that high-activity sulfhydryl groups can be formed; finally, the wool fabric where TCEP pre-processing is performed is soaked in an IDMA configured solution, C=C in IDMA molecules and S-H on wool protein molecules react through click chemistry, and IDMA quaternary ammonium salt is introduced into the wool protein molecules through a chemical bonding method. The processed wool fabric has the antibacterial and anti-static functions. The processing method is easy to operate, excellent in performance, lasting in effect and can improve the mechanical strength of the fabric.

The invention relates to a method for providing an anti-felting function for a wool fabric. The method utilizes tris(2-carboxyethyl)phosphine (TCEP) to pre-treat a wool fabric so that disulfide bonds in wool fiber keratin are broken to form mercapto groups with high activity. 2-propenoic acid-(2-hydroxy-1,3-propanediyl)bis[oxy(2-hydroxy-3,1-propanediyl)]ester (GDA) and the wool fabric pre-treated by TCEP undergo a click chemical reaction, in other word, C-C double bonds and the mercapto groups undergo a reaction so that a molecular chain rich in hydroxyl is introduced into wool protein molecules by a medical bonding method. The treated product has an anti-felting function, realizes a certain degree of machine washing, has good size stability and has a certain durability by chemical treatment. The method has substantial effects, belongs to a chlorine-free treatment method and is safe and environmentally friendly.

The invention relates to a method for determining the content of monomer aromatic hydrocarbons in gasoline through gas chromatography to mainly solve problems of large method establishing difficulty and large valve switching system dead volume of present chromatographic methods. The method, which allows an analyte which is the gasoline to be analyzed through a flow switching center cutting chromatographic device, concretely comprises the following steps: injecting the gasoline sample from a chromatographic shunting introduction port (3), pre-separating through a chromatographic column (4), cutting benzene, an internal standard substance and toluene to a chromatographic column (6) through a flow switching center cutting system (10), further separating, and detecting through a hydrogen flame ionization detector (5); and detecting other aromatic hydrocarbon components through allowing them to enter a damping column (2) after they are separated by the chromatographic column (4), wherein the chromatographic column (4) is TCEP[1,2,3-tri(2-cyanethoxy)]propane capillary chromatographic column; and the chromatographic column (6) is a nonpolar chromatographic column. The method well solves the problems through above technical scheme, and can be applied to the industrial production about the chromatographic analysis of gasoline samples.

The invention discloses a kit for rapidly extracting a nucleic acid in an amniotic fluid and chorionic tissue sample. The kit is prepared from the following components: a magnetic bead pyrolysis binding solution, magnetic beads, a magnetic bead cleaning solution 1, a magnetic bead cleaning solution 2 and a magnetic bead eluent. A 96-hole pre-subpackaging deep-hole plate mode is adopted, and an automatic nucleic acid extractor can be matched to automatically extract the nucleic acid. A TCEP additive is added into the magnetic bead pyrolysis binding solution, so that the nucleic acid extraction efficiency is greatly improved.

The invention relates to a manufacture method for PVC conveyor belt materials, which is technically characterized in that slurry is formed by mixing paste resin, disalt, threesalt, antimony trioxide, zinc borate, TCEP (also called phosphate tribasic (2-chloroethyl) ester), DOP (dioctyl phthalate), paraffin, carbon black according to a certain ratio; and an adhesive which is a mixture of resorcinol and urotropine with a certain ratio is added into a beating tank after being fully grinded by colloid, and is stirred by high speed to be uniformly mixed with the slurry. When the slurry is prepared, the adhesive is added according to the amount of 1 percent, and a belt body which is made by plasticization after the adhesive is added into the slurry ensures that gum and a core body are tightly combined, can not loose and is not easy to be torn, thereby the service life of the conveyor belt is prolonged by more than one time to obtain favorable effect.

The invention discloses a method for synthesizing linaclotide, and relates to the field of medicine synthesis. The method mainly comprises the following steps: improving a linear cyclizing system of linaclotide, cyclizing crude linear peptide by using a ammonium carbonate/DMSO/aqueous solution together with hydroquinone or TCEP so as to obtain a crude linaclotide product, and purifying the crude linaclotide product, thereby obtaining a finished product. By improving the cyclizing system, the yield of linaclotide is increased, the cyclizing time is shortened, the cost of raw materials is lowered, and the method is applicable to industrial production.

The invention discloses a P-rGO-C60 nanocomposite material and a P-rGO-C60-Pt@Au nanocomposite material, and also discloses an electrochemical DNA aptamer sensor for SDM detection, wherein the electrochemical DNA aptamer sensor is prepared by the following method: 1) treating an SDM aptamer by using a TCEP solution for standby application; 2) polishing a glassy carbon electrode into a mirror surface, processing the electrode, and drying for standby application; 3) electrochemically cleaning the electrode, swashing with water, and drying; 4) dropwise adding 6-10 [mu]L of P-rGO-C60 solution intothe surface of the glassy carbon electrode, drying, dropwise adding a GOD solution to the electrode; 5) dropwise adding the P-rGO-C60-Pt@Au solution to the electrode surface, and drying at room temperature; 6) taking an SDM binding aptamer, dropwise adding on the electrode, and incubating at room temperature; and 7) immersing the electrode in the GOD solution, and thus obtaining the product. A method for detecting the SDM by using the sensor is also disclosed.

The invention discloses a method and a reagent of separating an exosome from urine. The reagent is a mixed solution of polyethylene glycol and tri-(2-carboxyethyl) phosphine (TCEP). The experimental method sequentially comprises the steps of centrifuging the urine, mixing the reagent according to a certain proportion, adding the mixed reagent into the urine according to a certain proportion, performing mixing, standing overnight at 4 DEG C, after centrifugation the next day, discarding supernatant, and obtaining a sediment, namely the exosome. The method and the regent have the advantages that the exosome in the urine is high in yield and purity, the operation is simple, and the cost is low.

The invention provides a lithium-sulfur battery sandwich comprising carbon nanotube conductive paper and a shearing agent dispersed in the carbon nanotube conductive paper; the shearing agent is one or more of dithiothreitol, glutathione, TCEP (tris (2-carbonylethyl) phosphate hydrochloride) and mercaptoethanol (ME). After the lithium-sulfur battery is prepared by utilizing the sandwich layer withthe shearing agent, the shearing agent shears the high-order polysulfide, thereby preventing the dissolution of the high-order polysulfide. The porosity of carbon nanotube conductive paper can be more conducive to the adsorption of polysulfides to prevent their migration to the negative electrode. The physico-chemical synergism of the shearing agent and carbon nanotube conductive paper inhibits the shuttle effect and improves the electrochemical performance of lithium sulfide batteries. According to the description of the examples, the lithium sulfur battery provided by the present inventionhas better electrochemical performance than the lithium sulfur battery prepared by sandwiching without a shearing agent.

The invention discloses a bumper lower bar environment-friendly flame-retardant polyurethane foam material and a preparation method. The bumper lower bar environment-friendly flame-retardant polyurethane foam material is composed of acetonitrile polyester and a composite material in a mass ratio of 1:1.7, and the composite material is composed of the following components by mass: 25-28 parts of polyester 403, 35-45 parts of polyester 3360, 250-350 parts of glass fiber, 12-18 parts of polyether 4110, 2.5-3 parts of silicone oil, 1.3-1.8 parts of a catalyst HP-2, 1.5-1.9 parts of a catalyst A33, 3.6-3.9 parts of water, and 36-38 parts of a flame retardant TCEP, wherein the silicone oil is silicone oil AK-158. The preparation method includes: adding the components of the composite material into a reaction kettle and carrying out mixing reaction under a stirring speed of 130-150r/min for 4-5h to obtain the composite material, then mixing acetonitrile polyester and the composite material in a mass ratio of 1:1.7 evenly, thus obtaining the bumper lower bar environment-friendly flame-retardant polyurethane foam material. With the materials and the method provided by the invention, the prepared bumper lower bar environment-friendly flame-retardant polyurethane foam material has the advantages of flame retardance and high temperature resistance, high flame-retardant properties, high strength, long service life and environmental protection.

The invention discloses a pretreatment slurry for cashmere fabric digital printing and a printing technology of cashmere fabric digital printing. The pretreatment slurry is composed of sodium alginate paste, plant seed gelatine KCA-90 paste, TCEP, penetrant, urea, ammonium sulfate and water; the printing technology of cashmere fabric digital printing comprises the steps of sizing; conducting drying at the temperature of 40 DEG C for 10 min; conducting steaming at the temperature of 85 DEG C for 4-5 min; conducting cold washing at the room temperature for 10 min; conducting soaping at the temperature of 60 DEG C for 5 min with soaping agent A being 2g/L, and alginate lyase being 1 mg/L, and Na2CO3 being 0.5g/L; conducting hot washing at the temperature of 60 DEG C for 5 min; conducting cold washing at the room temperature for 5 min. By combining the pretreatment processing and the digital printing technology, the obtained printing cashmere fabric is high in tinctorial yield, uniform in color, free of phenomenon of grinning of white ground, clear in floral pattern margin, and good in color fastness.

The invention discloses a preparation method and an application of an electrochemical biosensor for detecting mercury ions based on the fast scanning cyclic voltammetry technology. The preparation method comprises the following steps: (1) adding Probe DNA1, Probe DNA2 and TCEP to rGO-NH2@AuNPs&Fc dispersion liquid to prepare functionalized reduced graphene oxide composite bio-nanomaterial dispersion liquid; (2) adding a solution containing Hg2+ to the functionalized reduced graphene oxide composite bio-nanomaterial dispersion liquid, uniformly mixing the solution, dripping the mixture on a gold electrode carrying Probe DNA3, incubating for 0.5 to 1 h under constant temperature and humidity conditions of 37 DEG C, and cleaning the electrode with a PBS buffer solution to obtain the electrochemical biosensor for detecting the mercury ions based on the fast scanning cyclic voltammetry technology. The electrochemical biosensor can be applied to the detection of the mercury ions based on thefast scanning cyclic voltammetry technology, and has the advantages of high sensitivity, high selectivity and simple and fast operation.

The invention discloses a method for quickly extracting, purifying and detecting protein in hair. The method is characterized by comprising the following steps: (1) classifying samples and respectively shearing the samples into small sections; (2) cleaning for 20 min with 0.15 percent nonylphenol polyoxyethylene ether (9) at the temperature of 60 DEG C and then sipping up; (3) cleaning for 5 min with dd water at the temperature of 60 DEG C and then sipping up; (4) cleaning for 20 min with methylene dichloride and ethanol at the room temperature and then sipping up; (5) cleaning for 5 min with the dd water at the temperature of 60 DEG C and then sipping up; (6) cleaning for 30 min with 0.1 M KOH at the room temperature and then sipping up; (7) cleaning for 5 min with the dd water at the temperature of 60 DEG C and then sipping up; (8) grinding with liquid nitrogen after water soaking; (9) extracting: overnight slaking after adding 7M urea of 200 [mu] L, 2M , 50mM Tris and 50mM TCEP (pH4) at the room temperature; (10) purifying.

The invention discloses an HSAB theory based sulfhydryl cotton post-modification material as well as preparation and application thereof, and belongs to the technical field of analytical chemistry. The HSAB theory based sulfhydryl cotton post-modification material is sulfhydryl cotton for modifying Ni<2+>, Cu<2+>, Pd<2+> or Au on the sulfhydryl cotton, wherein the preparation of the sulfhydryl cotton for modifying Ni<2+>, Cu<2+> or Pd<2+> comprises the following steps: dissolving tris(hydroxymethyl) aminomethane, sodium chloride, Ni<2+> salt, Cu<2+> salt or Pd<2+> salt in water; adding TCEP-treated sulfhydryl cotton, and sealing for reaction after full infiltration; cleaning and drying the products with deionized water. The preparation of the sulfhydryl cotton for modifying Au comprises the following steps: adding TCEP-treated sulfhydryl cotton to nano-gold solution, and sealing for reaction after full infiltration; drying the products after cleaning with deionized water. The HSAB theory based sulfhydryl cotton post-modification material has the advantages of simple operation, less solvent consumption and fast speed, and is especially suitable for the analysis of biological samples.

The invention discloses a semi-hard environment-friendly highly-flame-retardant elastomer foam material and preparation method thereof. The material is prepared from isocyanate and a composite material according to a mass ratio of 1:0.9. The composite material is prepared from, by mass, 25-28 parts of polyester 403, 35-45 parts of polyester 3360, 38-45 parts of polyester 330L, 12-18 parts of polyether 4110, 2.5-3.0 parts of silicon oil AK158, 1.3-1.8 parts of a catalyst HP-2, 1.5-1.9 parts of a catalyst A33, 3.6-3.9 parts of water and 36-38 parts of a flame retardant TCEP. By adoption of the materials and the method, the prepared foam material is excellent in flame retardation performance and less prone to combustion when encountering with fire, and accordingly fireproof safety and personal safety are guaranteed. Oil pots made from the foam material are quite light in weight and meet the requirement of convenience. Since raw materials of the foam material are all environment-friendly materials, release of toxic and harmful substances to human bodies is avoided, and the requirement of environment friendliness is met.