The invention relates to an anti-counterfeiting printing method by using invisible two-dimensional codes. The anti-counterfeiting printing method is characterized in that at least four printing
layers for printing are adopted, and comprises the following steps of: firstly, providing a digital serial number and splitting the serial number into three fields of which information is assigned to three groups of two-dimensional codes; secondly, printing one group of two-dimensional codes on a first layer by using aqueous ink black materials; thirdly,
overprinting a grey isolating layer on the first two-dimensional code layer by using
infrared non-absorbent materials to form a second layer; fourthly,
overprinting a second group of two-dimensional codes on the second layer of the grey isolating layer by using the
infrared non-absorbent materials to form a third layer; and finally,
overprinting a third group of two-dimensional codes on the third two-dimensional code layer by using
ultraviolet absorption materials to form a fourth layer. The method has the advantages of eliminating the possibility of
imitation by scanning the two-dimensional codes and achieving relatively higher use value because the overprinting is performed in the same area in an
offset printing and digital jet printing way and the upper printing layer covers the lower layer naturally.