Kit for rapidly extracting nucleic acid in amniotic fluid and chorionic tissue sample

A technology in kits and samples, applied in the field of nucleic acid rapid extraction kits, can solve the problems of low extraction efficiency, error-prone, tedious and complicated, etc.

Inactive Publication Date: 2017-05-24
GUANGZHOU HEAS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the column extraction method and the magnetic bead method do not use toxic and harmful substances such as phenol and chloroform in the process of extracting nucleic acids, they need to repeatedly open the cap to add liquid, discard liquid, and centrifuge steps, and frequently replace centrifuge tubes and pipette tips. The column

Method used

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  • Kit for rapidly extracting nucleic acid in amniotic fluid and chorionic tissue sample
  • Kit for rapidly extracting nucleic acid in amniotic fluid and chorionic tissue sample
  • Kit for rapidly extracting nucleic acid in amniotic fluid and chorionic tissue sample

Examples

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Embodiment 1

[0044] Six amniotic fluid samples were taken and equally divided into four groups. The first group was extracted with the kit without additives in the magnetic bead lysis binding solution; the second and third groups were extracted with the kits with TCEP and DTT additives respectively added to the magnetic bead lysis binding solution, and the fourth group was extracted with Qiagen The company's QIAamp DNA Blood Mini Kit (50) - Cat. No. 51104 kit for extraction. The 4 groups of extracted nucleic acids were subjected to β-Actin amplification, and the 4 groups of PCR products were subjected to electrophoresis. The result is as image 3 , where A1-A6 lanes are the results without additives, B1-B6 lanes are the results of Qiagen kits, M lanes are 100bp Marker, C1-C6 lanes are the results of adding DTT additives, D1-D6 lanes are the results of adding TCEP additives the result of;

[0045] Table 2 corresponds to the concentration of nucleic acid obtained by extracting nucleic aci...

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Abstract

The invention discloses a kit for rapidly extracting a nucleic acid in an amniotic fluid and chorionic tissue sample. The kit is prepared from the following components: a magnetic bead pyrolysis binding solution, magnetic beads, a magnetic bead cleaning solution 1, a magnetic bead cleaning solution 2 and a magnetic bead eluent. A 96-hole pre-subpackaging deep-hole plate mode is adopted, and an automatic nucleic acid extractor can be matched to automatically extract the nucleic acid. A TCEP additive is added into the magnetic bead pyrolysis binding solution, so that the nucleic acid extraction efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid nucleic acid extraction kit in amniotic fluid and villi samples. The invention also relates to a method for extracting nucleic acid in amniotic fluid and villi samples by using the magnetic bead method. Background technique [0002] The basic unit of genetic characteristics of living organisms is nucleic acid, which is inseparable from the detection of nucleic acid in many fields such as genetic research, and the key premise is to extract effective and reliable nucleic acid, which is also the key in many nucleic acid molecular biology experiments. The key, such as qualitative PCR, molecular hybridization, real-time fluorescent quantitative PCR and other technologies. Nucleic acid plays an irreplaceable role in the whole molecular biology. [0003] In the prenatal diagnosis of pregnant women, nucleic acid testing of amniotic fluid and chorionic villi samples is very importan...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2563/143C12Q2563/149C12Q2523/308
Inventor 刘淑园陈华云肖湘文丁渭张天海邓金萍黄爽曾烨
Owner GUANGZHOU HEAS BIOTECH CO LTD
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