28 results about "Molecular hybridization" patented technology

The invention discloses a (-)-menthol group-containing nicotinamide derivative with antifungal activity as well as a preparation method and application thereof, and relates to the technical field of synthesis of agricultural chemicals. The derivative has a structure as shown in a general formula (I), in the formula, n is an integer from 1 to 5, and R1, R2, R3 and R4 are specific substituent groups. Through a molecular hybridization strategy, the (-)-menthol, the N-fatty acyl piperazine bridge and the nicotinamide fragment are covalently linked, and a compound with a brand new structure is created. A biological activity test shows that the series of compounds have remarkable inhibitory activity on various plant pathogenic fungi, especially sclerotinia sclerotiorum and valsa mali of apple trees, and are low in cytotoxicity. Molecular docking research proves that the action target of the gene is succinate dehydrogenase (SDH). The invention provides a valuable leading structure for developing novel SDHI bactericides.

1. Name of the Design Product: Automatic Nucleic Acid Hybridization Instrument (YH96pro). 2. Use of the Design Product: For automatic hybridization of nucleic acids. 3. Design Key Points of the Design Product: The combination of shape and pattern. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The application relates to the technical field of aspirin pharmaceutical chemistry, in particular to an aspirin-sulfonamide hybrid, a preparation method and application thereof. The preparation method can synthesize a plurality of novel aspirin-sulfonamide hybrids by taking piperazine as a bridge, and by a three-step continuous method of "acyl chlorination, sulfonamidation and N-acylation" according to a "molecular hybridization principle". In the process, only one separation and purification is performed, and the preparation steps are simple. The aspirin-sulfonamide hybrid prepared by the method is most effective on human non-small cell lung cancer cells A549, and the activity is more than 33 times higher than that of a parent aspirin, and is similar to the activity of an anticancer drug irinotecan. The aspirin-sulfonamide hybrid 3k prepared by the method has a certain inhibitory effect on various cancer cells. The hybrid can induce human non-small cell lung cancer A549 cell apoptosis in a concentration-dependent manner, and can induce human non-small cell lung cancer A549 cell cycle arrest in the G0 / G1 phase, and inhibit cell growth.

The present application relates to the field of biotechnology and molecular detection, in particular to a plant and pathogenic bacteria interaction gene expression analysis method based on UMI tag, comprising: obtaining interaction sample total nucleic acid extract; and constructing a molecular hybridization system with host-specific chimeric blocking probes containing locked nucleic acid modification and 3' end dideoxy modification, and reverse transcription primers; through temperature rising and denaturation and temperature reduction and annealing, the blocking probes are combined with host high abundance nucleic acid to form a hybridization blocking complex, and the primers are combined with pathogenic bacteria nucleic acid; template switching oligonucleotides with unique molecular identifier sequences and reverse transcriptase are added, the host extension is blocked, and UMI is introduced into the pathogenic bacteria complementary chain, and an interaction expression library is generated; after sequencing, the pathogenic bacteria effective reads and the host invalid reads are counted, and the effective interaction signal signal-to-noise ratio is output; the present application can preferentially capture pathogenic bacteria transcripts in the high background of host RNA.

The invention discloses a molecular hybridization instrument and an operation device, the operation device comprises a displacement mechanism, a liquid adding and discharging mechanism and an optical detection mechanism, and the liquid adding and discharging mechanism and the optical detection mechanism are both connected with the displacement mechanism; the shifting mechanism is used for shifting the liquid adding and discharging mechanism and the optical detection mechanism to each sample storage group along the arrangement direction of the sample storage groups in the sample box; the liquid adding and discharging mechanism is used for extracting waste liquid in each sample storage position at the same time when the sample storage group is moved, or injecting a reagent into each sample storage position at the same time; and the optical detection mechanism is used for performing optical detection on each sample storage position while moving to the sample storage group. According to the operation device, the detection efficiency can be greatly improved, meanwhile, due to the fact that liquid adding and discharging and detection are conducted through the liquid adding and discharging mechanism and the optical detection mechanism respectively, structures with different use functions operate independently and do not interfere with one another, the influence of frequent action of the liquid adding and discharging mechanism on the stability of the optical detection mechanism is reduced, and stable operation of the whole machine is guaranteed.

The invention discloses a gene detection platform based on a micro-fluidic chip, which integrates an operation platform, the micro-fluidic chip, a control system, an automatic pipetting device and the like, and is characterized in that a plurality of independent detection channels are arranged on the micro-fluidic chip; each detection channel is provided with a PCR amplification chamber, an amplification product analysis chamber, a two-way pump, a waste liquid collection chamber, a three-way electromagnetic valve and the like, and the detection platform can realize full-automatic liquid preparation, nucleic acid extraction (whole blood mode, omitting the step), PCR amplification, denaturation, molecular hybridization and the like on the basis of the micro-fluidic chip. And the whole process of the steps of result storage, interpretation and the like is automatic, so that the integrated detection requirement of'sample feeding and result outputting 'is met. Meanwhile, the kit is applied to the detection of thalassemia genes, and specific primers and probes are designed and screened to obtain the thalassemia detection kit, and the kit has the advantages of being convenient to operate, high in detection precision, low in cost, visual in result interpretation and the like.

The application belongs to the technical fields of synthesis of compounds and pharmaceutical applications, and discloses an anti-tumor pterostilbene piperazine conjugate as well as a preparation method and application thereof. The pterostilbene, piperazine and various substituents are spliced in one molecule by using a molecular hybridization method, a novel anti-tumor pterostilbene piperazine conjugate is synthesized, and it is found through anti-tumor activity research that the anti-tumor activity of the conjugate is obviously higher than that of the two pharmacophores alone, and the conjugate has the potential to become a novel anti-tumor drug. The pterostilbene piperazine conjugate is synthesized, and it is found for the first time that the compound has good anti-tumor activity, can significantly inhibit the proliferation of various tumor cells in vitro, including hepatocarcinoma, triple-negative breast cancer, colon cancer and ovarian cancer cells, has little toxicity to normal cells, and can inhibit the migration ability of tumor cells. Therefore, the compound synthesized by the application has good anti-tumor application prospect.

The present invention relates to the field of biotechnology, and in particular to the preparation and application of a CD56 protein-specific nanoantibody-HRP fusion protein. The present application discloses a CD56 protein-specific nanoantibody-HRP fusion protein and a preparation method thereof, as well as the application of the fusion protein in preparing a product for detecting CD56 protein-specific antibodies in serum. The HRP-fused nanoantibody prepared in the present application constructs a fusion protein of a primary antibody and a labeled HRP through genetic engineering, converting two molecular hybridizations into one molecular hybridization, which can greatly shorten the duration of enzyme-linked immunosorbent assay and improve the accuracy. Compared with traditional antibodies, nanoantibodies are easier to genetically manipulate and can be coupled with a variety of labeled enzymes.

The invention provides a 5alpha, 8alpha-peroxy ergosterol-quaternary ammonium salt derivative as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The preparation method comprises the following steps: firstly, synthesizing a peroxy-ergosterol bromate derivative by taking 5alpha, 8alpha-peroxy-ergosterol as a raw material; then, based on the molecular hybridization theory, a series of side chains with different amino substituent groups are introduced to the peroxy ergosterol bromate derivative, and a quaternary ammonium salt structure is constructed. A series of 5 alpha, 8 alpha-peroxy ergosterol-quaternary ammonium salt derivatives with clinical anti-tumor application potential are designed and synthesized. An anti-tumor activity test shows that the obtained derivative has different degrees of inhibiting effects on tumor cells of human lung cancer, liver cancer, breast cancer and the like and shows a remarkable inhibiting effect on human breast cancer MDA-MB-231 cells, and the anti-tumor activity of the derivative with the most excellent activity is improved by 69 times compared with that of a lead compound 5alpha, 8alpha-peroxy ergosterol.

The invention discloses a solid-phase gene chip molecular hybridization instrument which comprises a bottom plate, a heat cover, a swing driving assembly and a swing fulcrum assembly, and heating elements are arranged in the bottom plate and the heat cover; the control unit is used for controlling the temperature of the heating element and the swing driving assembly, the heating element emits heat at the same time, and the swing driving assembly drives the bottom plate, the heat cover and the molecular hybridization container to swing around the swing fulcrum assembly in a reciprocating mode at the same time. The molecular hybridization container has the advantages that on the premise that trace liquid does not lose, a variable-speed reciprocating motion field of the molecular hybridization container is constructed through a brand-new asymmetric swing mode with fluid mechanics gain, high-frequency shear force is induced on a solid-liquid interface, and diffusion limitation of the solid-liquid interface under a trace system is overcome; meanwhile, high-precision temperature control and anti-condensation effects are achieved through solid heat transfer on the upper side and the lower side, and the temperature non-uniformity and the fringe effect in traditional solid-phase hybridization are effectively solved.

The present disclosure relates in some aspects to methods, compositions, and kits of processing or analyzing a sample. A method for processing a sample may comprise providing probes with barcodes to generate a composite barcode, detecting the barcodes to determine a spatial location and hybridizing probes to analytes (e.g., an RNA molecule) and performing sequencing.

The invention provides a cyclopentene chromene-cinnamate compound as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis. According to the invention, mangiferin is taken as a guide, and a cyclopentenochromene structure is designed and synthesized from cyclopentenopyrane and a chromene skeleton by using molecular hybridization and skeleton transition strategies. According to the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment, the cinnamic acid fragment with pesticide activity is grafted to the 8-site of cyclopentenochromene, the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment is synthesized, and the obtained compound has good insecticidal activity, bactericidal activity and plant virus resisting activity.

The utility model discloses an easy-to-install elisa plate clamp for a molecular hybridization furnace, and relates to the technical field of elisa plate clamps. The ELISA plate clamping device comprises a bottom plate and a clamping part which is movably arranged on the surface of the bottom plate and is used for clamping an ELISA plate, the clamping part comprises a bearing plate with a boss, a pair of limiting baffle rings arranged on the opposite sides and rubber blocks installed on the inner side walls of the limiting baffle rings. The boss is sleeved with a bottom shell of the elisa plate, a pair of opposite side edges of the bottom shell are located between the boss and the limiting baffle ring, and the side edges of the elisa plate are extruded through rubber blocks on the inner side wall of the limiting baffle ring and then clamped. The elisa plate clamping device can meet the requirement for clamping the elisa plates in various forms and sizes; and compared with an elastic sheet structure, the situation that hands are cut is avoided, and the leakage of built-in substances in a hybridization tube carried on the elisa plate can be avoided.

The application discloses a reusable spatial index template chip, a replicable template chip, a spatial transcriptome chip and a preparation method and application thereof. A composite index library compatible with a general sequencing platform is designed, spatial barcode mapping is completed on a commercial chip to obtain a spatial index template chip. Through molecular hybridization and enzymatic extension technology, a known coordinate array is copied to a receptor chip, and multiple generations of copying are supported, so that a batch spatial transcriptome chip preparation system sharing the same spatial coordinate atlas is constructed. The application realizes deep compatibility with a standard sequencing platform through composite index library design, decouples a spatial capture carrier from an original sequencing chip, significantly reduces experimental starting cost, and provides a large-scale solution for high-throughput and low-cost spatial omics large-scale application.

A complex for a toehold exchange system is provided. The complex comprised a substrate molecule and an output molecule. The substrate molecule and output molecule each comprise: a plurality of complementary regions where the substrate molecule and the output molecule are hybridised; and a non-complementary region where the substrate molecule and output molecule are not hybridised, the non-complementary region being located between complementary regions. The substrate molecule comprises a first exposed toehold region for hybridisation to a catalyst molecule and a second covered toehold region for hybridisation to a fuel molecule, and wherein the second covered toehold region comprises the region of non- complementarity. A toehold exchange system comprising the complex is also provided.

Provided herein is an mRNA complex comprising an mRNA molecule and at least one engineered oligonucleotide hybridized to the mRNA molecule, wherein said engineered oligonucleotide is hereafter referred to as the Str-O-Nuc.

Embodiments provide a construction method for a high-throughput sequencing library, a kit, and a use. The method comprises: using a Tn5 transposase complex to randomly break a DNA in a genome sample to obtain a DNA breakage product; carrying out molecular hybridization on an RNA enrichment probe and the DNA breakage product, and using streptavidin magnetic beads to collect an RNA-DNA complex in a molecular hybridization product; and carrying out methylation treatment and PCR amplification on the RNA-DNA complex to obtain a sequencing library. The method achieves directed enrichment of a target methylation site in the genome sample; when a new target methylation site needs to be added, an RNA enrichment probe is simply designed for same; when the target methylation site that has been proven to have a poor effect needs to be deleted, the RNA enrichment probe corresponding thereto is simply removed; and by means of the directed enrichment of the target methylation site, the concentration requirement of the genome sample is reduced, and trace detection is facilitated.

Provided herein is an mRNA complex comprising an mRNA molecule and at least one engineered oligonucleotide hybridized to the mRNA molecule, wherein said engineered oligonucleotide is hereafter referred to as the Str-O-Nuc.

Provided herein is an mRNA complex comprising an mRNA molecule and at least one engineered oligonucleotide hybridized to the mRNA molecule, wherein said engineered oligonucleotide is hereafter referred to as the Str-O-Nuc.

The invention relates to the related technical field of molecular hybridization, and discloses a molecular hybridization instrument for optimizing reagent mixing treatment effect, which comprises a hybridization instrument body, a reaction cabin is arranged in the middle of the hybridization instrument body, a cabin door is rotatably arranged on one side of the reaction cabin, a control panel is arranged on one side of the outer part of the hybridization instrument body, and a mixing assembly is arranged in the reaction cabin; two first circular plates can be driven to rotate and move back and forth through rotation and back-and-forth movement of a circular tube, and the two first circular plates rotate and move back and forth to drive a plurality of hybridization tubes to rotate and move back and forth, so that the effect of uniformly mixing reagents in the hybridization tubes is improved; a plurality of second sliding plates, third push rods and arc-shaped plates move back and forth, so that a plurality of hybridization tubes shake back and forth in a plurality of grooves in the radial direction, and the effect of uniformly mixing reagents in the hybridization tubes is further improved.

The invention discloses a method for preparing coding microspheres based on a microarray chip and a product and application thereof, and the method comprises the following steps: spraying a coding sequence to the surface of a glass slide to construct a coding microarray chip; arranging the gel microspheres fixed with the amplification primers on the surface of a coding microarray chip in a single layer, so that the microspheres are tightly combined with the surface of the chip; copying a coding sequence on the surface of the chip to the surface of the microsphere by using a molecular hybridization and extension technology; eluting the microspheres with the copied and transferred coding sequences from the surface of the chip, carrying out bridge type PCR amplification reaction, and copying a large amount of the coding sequences transferred from the surface of the chip until the whole microspheres are full of the coding sequences; and after amplification is completed, one complementary chain is cut off, so that the complete coding microspheres are obtained. The chip can be reused, the cost is obviously reduced, the operation is simple, convenient and flexible, and the mass production is high.

Methods of single molecule sequencing are provided. The primer may be hybridized to a template molecule to form a hybridized template. The sequence of a first region of the template molecule can be determined by extending the primer through the first region using labeled nucleotides. The primer may be extended through a second region of the template using an unlabeled nucleotide. The sequence of a third region of the template molecule can be determined by extending the primer through the third region using a labeled nucleotide. The sequence of the second region of the template molecule may be determined by comparing the sequence of the first region and the sequence of the third region to a reference sequence.

The present invention relates to a novel CRISPR system comprising i) at least one nucleotide sequence encoding at least one Cas13 protein; and ii) at least one gRNA or at least one nucleotide sequence encoding said at least one gRNA capable of hybridizing with one or more viral target RNA molecules, wherein said system comprises a viral 5′ UTR or a nucleotide sequence encoding said 5′ UTR and / or a viral 3′ UTR or a nucleotide sequence encoding said viral 3′ UTR, wherein a viral replicase recognition sequence is comprised in at least any one of said 5′ UTR or in the nucleotide sequence encoding said 5′ UTR, or said 3′ UTR or in the nucleotide sequence encoding said 3′ UTR, and wherein said system does not comprise a nucleotide sequence encoding a viral replicase and wherein said viral replicase recognition sequence is from the same RNA virus as the one or more viral target RNA molecules. The present invention also relates to a delivery system comprising the novel system and a composition comprising the novel system or the delivery system. The present invention further relates to the medical use of the novel system or in particular to the system for use in a method of preventing or treating a viral disease in a subject. Additionally, the present invention also relates to a kit comprising the novel system and to a method of producing the novel system.

The invention relates to the field of molecular biology, in particular to a detection method and device based on an FRET effect. According to the present invention, the linear relationship between the energy transfer efficiency (E) of the intramolecular FRET effect and the reciprocal R-1 of the linear distance between the intramolecular donor and the intramolecular receptor is firstly found, and the detection method based on the finding is provided, such that the mutation nucleic acid can be effectively detected so as to easily reduce the detection failure caused by the microbial mutation, the kit can realize early evolution early warning of viruses and real-time monitoring of virus variation, and can be widely applied to multiple fields of fluorescent mice, antibody preparation, molecular hybridization, genotyping, cancer mutation screening, clinical pathogen drug-resistant mutation detection and the like.

1. Name of the product in this design: Fully Automated Nucleic Acid Molecular Hybridization Instrument. 2. Purpose of this design: for nucleic acid molecular hybridization. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 2.

The present application relates to the field of molecular biology, in particular, to a detection method and device based on FRET effect. The present application first discovers that the energy transfer efficiency of intramolecular FRET effect E ) and the reciprocal of the linear distance of intramolecular donor and acceptor R ‑1 present a linear relationship, and proposes a detection method based on the discovery, which can effectively detect mutant nucleic acids, is beneficial to reduce the detection failure caused by microbial mutation, can realize early evolution warning and real-time monitoring of virus variation, and can be widely applied to multiple fields such as fluorescent mouse, antibody preparation, molecular hybridization, genotyping, cancer mutation screening, clinical pathogen drug resistance mutation detection and the like.

This invention discloses a method, system, computer equipment, and readable storage medium for signature image DNA encoding storage and anti-counterfeiting verification based on feature learning, belonging to the field of electronic information technology. The method includes: extracting high-dimensional discriminative feature vectors from the signature image to be verified using a feature extractor; inputting the vectors into a trained sequence encoder to generate a DNA sequence; synthesizing a reverse complementary sequence to prepare a labeled probe; hybridizing the probe with the registered DNA sequence of the target signer; measuring the hybridization yield; if the yield exceeds a preset threshold, the signature is determined to be genuine; otherwise, it is a forged signature. This invention is the first to integrate handwritten signature anti-counterfeiting with DNA storage. Through three core components—a feature extractor, a sequence encoder, and a hybridization predictor—and multi-task joint training, genuine signature pairs are encoded as high-hybridization-yield sequences, while genuine and forged signature pairs are encoded as low-hybridization-yield sequences, achieving physical-level anti-counterfeiting verification based on molecular hybridization. The verification process of this invention is inherently parallel, energy-efficient, and highly accurate, meeting the needs of practical anti-counterfeiting applications.

A method for evaluating the sample permeabilization effect in a microbial single-cell sequencing experiment, which belongs to the technical field of molecular hybridization detection. The evaluation method comprises the following steps: (1) fixing a microbial cell; (2) performing permeabilization treatment on the fixed cell; (3) adding the permeabilized cell sample to a glass slide for baking; (4) adding a probe for in-situ fluorescence probe hybridization; (5) washing same, and counterstaining the cell nucleus; and (6) evaluating the cell permeabilization effect according to the fluorescence intensity of the sample under a fluorescence microscope. The method for evaluating the permeabilization effect of a microbial cell sample can detect the correlation between the permeabilization time and the sample permeabilization effect in the case of a single microbial cell or a mixed microbial cell, thereby determining the optimal permeabilization treatment time of the sample and stabilizing the rRNA proportion and data output of the sample.