13 results about "Molecular hybridization" patented technology

The invention discloses a (-)-menthol group-containing nicotinamide derivative with antifungal activity as well as a preparation method and application thereof, and relates to the technical field of synthesis of agricultural chemicals. The derivative has a structure as shown in a general formula (I), in the formula, n is an integer from 1 to 5, and R1, R2, R3 and R4 are specific substituent groups. Through a molecular hybridization strategy, the (-)-menthol, the N-fatty acyl piperazine bridge and the nicotinamide fragment are covalently linked, and a compound with a brand new structure is created. A biological activity test shows that the series of compounds have remarkable inhibitory activity on various plant pathogenic fungi, especially sclerotinia sclerotiorum and valsa mali of apple trees, and are low in cytotoxicity. Molecular docking research proves that the action target of the gene is succinate dehydrogenase (SDH). The invention provides a valuable leading structure for developing novel SDHI bactericides.

The application relates to the technical field of aspirin pharmaceutical chemistry, in particular to an aspirin-sulfonamide hybrid, a preparation method and application thereof. The preparation method can synthesize a plurality of novel aspirin-sulfonamide hybrids by taking piperazine as a bridge, and by a three-step continuous method of "acyl chlorination, sulfonamidation and N-acylation" according to a "molecular hybridization principle". In the process, only one separation and purification is performed, and the preparation steps are simple. The aspirin-sulfonamide hybrid prepared by the method is most effective on human non-small cell lung cancer cells A549, and the activity is more than 33 times higher than that of a parent aspirin, and is similar to the activity of an anticancer drug irinotecan. The aspirin-sulfonamide hybrid 3k prepared by the method has a certain inhibitory effect on various cancer cells. The hybrid can induce human non-small cell lung cancer A549 cell apoptosis in a concentration-dependent manner, and can induce human non-small cell lung cancer A549 cell cycle arrest in the G0 / G1 phase, and inhibit cell growth.

The present application relates to the field of biotechnology and molecular detection, in particular to a plant and pathogenic bacteria interaction gene expression analysis method based on UMI tag, comprising: obtaining interaction sample total nucleic acid extract; and constructing a molecular hybridization system with host-specific chimeric blocking probes containing locked nucleic acid modification and 3' end dideoxy modification, and reverse transcription primers; through temperature rising and denaturation and temperature reduction and annealing, the blocking probes are combined with host high abundance nucleic acid to form a hybridization blocking complex, and the primers are combined with pathogenic bacteria nucleic acid; template switching oligonucleotides with unique molecular identifier sequences and reverse transcriptase are added, the host extension is blocked, and UMI is introduced into the pathogenic bacteria complementary chain, and an interaction expression library is generated; after sequencing, the pathogenic bacteria effective reads and the host invalid reads are counted, and the effective interaction signal signal-to-noise ratio is output; the present application can preferentially capture pathogenic bacteria transcripts in the high background of host RNA.

The application belongs to the technical fields of synthesis of compounds and pharmaceutical applications, and discloses an anti-tumor pterostilbene piperazine conjugate as well as a preparation method and application thereof. The pterostilbene, piperazine and various substituents are spliced in one molecule by using a molecular hybridization method, a novel anti-tumor pterostilbene piperazine conjugate is synthesized, and it is found through anti-tumor activity research that the anti-tumor activity of the conjugate is obviously higher than that of the two pharmacophores alone, and the conjugate has the potential to become a novel anti-tumor drug. The pterostilbene piperazine conjugate is synthesized, and it is found for the first time that the compound has good anti-tumor activity, can significantly inhibit the proliferation of various tumor cells in vitro, including hepatocarcinoma, triple-negative breast cancer, colon cancer and ovarian cancer cells, has little toxicity to normal cells, and can inhibit the migration ability of tumor cells. Therefore, the compound synthesized by the application has good anti-tumor application prospect.

The invention discloses a solid-phase gene chip molecular hybridization instrument which comprises a bottom plate, a heat cover, a swing driving assembly and a swing fulcrum assembly, and heating elements are arranged in the bottom plate and the heat cover; the control unit is used for controlling the temperature of the heating element and the swing driving assembly, the heating element emits heat at the same time, and the swing driving assembly drives the bottom plate, the heat cover and the molecular hybridization container to swing around the swing fulcrum assembly in a reciprocating mode at the same time. The molecular hybridization container has the advantages that on the premise that trace liquid does not lose, a variable-speed reciprocating motion field of the molecular hybridization container is constructed through a brand-new asymmetric swing mode with fluid mechanics gain, high-frequency shear force is induced on a solid-liquid interface, and diffusion limitation of the solid-liquid interface under a trace system is overcome; meanwhile, high-precision temperature control and anti-condensation effects are achieved through solid heat transfer on the upper side and the lower side, and the temperature non-uniformity and the fringe effect in traditional solid-phase hybridization are effectively solved.

The present disclosure relates in some aspects to methods, compositions, and kits of processing or analyzing a sample. A method for processing a sample may comprise providing probes with barcodes to generate a composite barcode, detecting the barcodes to determine a spatial location and hybridizing probes to analytes (e.g., an RNA molecule) and performing sequencing.

The invention provides a cyclopentene chromene-cinnamate compound as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis. According to the invention, mangiferin is taken as a guide, and a cyclopentenochromene structure is designed and synthesized from cyclopentenopyrane and a chromene skeleton by using molecular hybridization and skeleton transition strategies. According to the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment, the cinnamic acid fragment with pesticide activity is grafted to the 8-site of cyclopentenochromene, the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment is synthesized, and the obtained compound has good insecticidal activity, bactericidal activity and plant virus resisting activity.

The application discloses a reusable spatial index template chip, a replicable template chip, a spatial transcriptome chip and a preparation method and application thereof. A composite index library compatible with a general sequencing platform is designed, spatial barcode mapping is completed on a commercial chip to obtain a spatial index template chip. Through molecular hybridization and enzymatic extension technology, a known coordinate array is copied to a receptor chip, and multiple generations of copying are supported, so that a batch spatial transcriptome chip preparation system sharing the same spatial coordinate atlas is constructed. The application realizes deep compatibility with a standard sequencing platform through composite index library design, decouples a spatial capture carrier from an original sequencing chip, significantly reduces experimental starting cost, and provides a large-scale solution for high-throughput and low-cost spatial omics large-scale application.

Provided herein is an mRNA complex comprising an mRNA molecule and at least one engineered oligonucleotide hybridized to the mRNA molecule, wherein said engineered oligonucleotide is hereafter referred to as the Str-O-Nuc.

Provided herein is an mRNA complex comprising an mRNA molecule and at least one engineered oligonucleotide hybridized to the mRNA molecule, wherein said engineered oligonucleotide is hereafter referred to as the Str-O-Nuc.

The invention discloses a method for preparing coding microspheres based on a microarray chip and a product and application thereof, and the method comprises the following steps: spraying a coding sequence to the surface of a glass slide to construct a coding microarray chip; arranging the gel microspheres fixed with the amplification primers on the surface of a coding microarray chip in a single layer, so that the microspheres are tightly combined with the surface of the chip; copying a coding sequence on the surface of the chip to the surface of the microsphere by using a molecular hybridization and extension technology; eluting the microspheres with the copied and transferred coding sequences from the surface of the chip, carrying out bridge type PCR amplification reaction, and copying a large amount of the coding sequences transferred from the surface of the chip until the whole microspheres are full of the coding sequences; and after amplification is completed, one complementary chain is cut off, so that the complete coding microspheres are obtained. The chip can be reused, the cost is obviously reduced, the operation is simple, convenient and flexible, and the mass production is high.

Methods of single molecule sequencing are provided. The primer may be hybridized to a template molecule to form a hybridized template. The sequence of a first region of the template molecule can be determined by extending the primer through the first region using labeled nucleotides. The primer may be extended through a second region of the template using an unlabeled nucleotide. The sequence of a third region of the template molecule can be determined by extending the primer through the third region using a labeled nucleotide. The sequence of the second region of the template molecule may be determined by comparing the sequence of the first region and the sequence of the third region to a reference sequence.

This invention discloses a method, system, computer equipment, and readable storage medium for signature image DNA encoding storage and anti-counterfeiting verification based on feature learning, belonging to the field of electronic information technology. The method includes: extracting high-dimensional discriminative feature vectors from the signature image to be verified using a feature extractor; inputting the vectors into a trained sequence encoder to generate a DNA sequence; synthesizing a reverse complementary sequence to prepare a labeled probe; hybridizing the probe with the registered DNA sequence of the target signer; measuring the hybridization yield; if the yield exceeds a preset threshold, the signature is determined to be genuine; otherwise, it is a forged signature. This invention is the first to integrate handwritten signature anti-counterfeiting with DNA storage. Through three core components—a feature extractor, a sequence encoder, and a hybridization predictor—and multi-task joint training, genuine signature pairs are encoded as high-hybridization-yield sequences, while genuine and forged signature pairs are encoded as low-hybridization-yield sequences, achieving physical-level anti-counterfeiting verification based on molecular hybridization. The verification process of this invention is inherently parallel, energy-efficient, and highly accurate, meeting the needs of practical anti-counterfeiting applications.