Microcystin test chip, kit and detection method

A microcystin and detection chip technology, which is applied in the field of microcystin detection chips, can solve the problems of affecting the monitoring/early warning of harmful algal blooms, the inability to pre-inspect for toxin-producing microcystis, and reducing the timeliness of biological monitoring. The effect of shortening the detection time, eliminating PCR amplification, and quickly correcting the accuracy
CN102199670AInactive Publication Date: 2011-09-28NINGBO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO UNIV
Publication Date
2011-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a microcystin test chip. The microcystin test chip comprises a substrate and probes combined on the substrate, wherein the probes are six specific probes related to six conserved region sequences in a microcystin synthetic gene cluster. The invention also discloses a kit provided with the test chip and a method for detecting microcystin by utilizing the kit. Compared with the prior art, the invention has the advantages that: a biochip and a molecular hybridization technology are combined, the fussy polymerase chain reaction (PCR) amplification is rejected, the detection time is shortened, and the high-throughput and high-sensitivity rapid detection can be performed.
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Description

Technical field

[0001] The invention relates to a microcystin detection chip. The invention also relates to a kit with the detection chip and a method for detecting microcystin using the kit. Background technique

[0002] Cyanobacteria are also known as "blue-green algae". In some nutrient-rich water bodies, certain cyanobacteria often multiply in summer to form "blooms". While causing the deterioration of water quality, certain "blooms" species also produce biologically toxic secondary metabolites called "cyanobacterial toxins." The release of these cyanobacteria toxins directly harms fish, humans and animals. Cyanobacteria toxins can be divided into four categories according to the different diseases that they ultimately cause: neurotoxins, liver toxins, cytotoxins and irritant toxins. Among the four types of toxins, the hepatic toxin, microcystin, is the most harmful to the public's health, and the serious one can cause its death. Therefore, scientists from various countrie...

Claims

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