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Microcystin test chip, kit and detection method

A microcystin and detection chip technology, which is applied in the field of microcystin detection chips, can solve the problems of affecting the monitoring/early warning of harmful algal blooms, the inability to pre-inspect for toxin-producing microcystis, and reducing the timeliness of biological monitoring. The effect of shortening the detection time, eliminating PCR amplification, and quickly correcting the accuracy

Inactive Publication Date: 2011-09-28
NINGBO UNIV
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Problems solved by technology

However, the above-mentioned various diagnostic methods all target the microcystin released into the water body, and cannot pre-judge the toxin before it is released from the algae cells into the water body
Potential toxigenic Microcystis cannot be pre-screened by these methods
As a result, the timeliness of biological monitoring is reduced, which greatly affects the monitoring / early warning of harmful algal blooms

Method used

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  • Microcystin test chip, kit and detection method
  • Microcystin test chip, kit and detection method
  • Microcystin test chip, kit and detection method

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Embodiment Construction

[0023] The present invention will be described in further detail below in conjunction with the embodiments of the drawings.

[0024] ①Sample DNA extraction: Centrifuge the water sample, discard the supernatant, and extract the metagenomic DNA in the centrifuged water sample with nucleic acid extractant combined with adsorption column method

[0025] ②Hybridization: Melt the hybridization solution and mix it with metagenomic DNA. After denaturation, place the chip in the hybridization box with an ice bath, cover with a cover slip, and inject the denatured ice bath hybridization solution through the sample hole, and close the hybridization box lid , Hybridize in the hybridizer;

[0026] ③Washing: the hybridized chip is cleaned and centrifuged by a washer-dryer;

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Abstract

The invention discloses a microcystin test chip. The microcystin test chip comprises a substrate and probes combined on the substrate, wherein the probes are six specific probes related to six conserved region sequences in a microcystin synthetic gene cluster. The invention also discloses a kit provided with the test chip and a method for detecting microcystin by utilizing the kit. Compared with the prior art, the invention has the advantages that: a biochip and a molecular hybridization technology are combined, the fussy polymerase chain reaction (PCR) amplification is rejected, the detection time is shortened, and the high-throughput and high-sensitivity rapid detection can be performed.

Description

Technical field [0001] The invention relates to a microcystin detection chip. The invention also relates to a kit with the detection chip and a method for detecting microcystin using the kit. Background technique [0002] Cyanobacteria are also known as "blue-green algae". In some nutrient-rich water bodies, certain cyanobacteria often multiply in summer to form "blooms". While causing the deterioration of water quality, certain "blooms" species also produce biologically toxic secondary metabolites called "cyanobacterial toxins." The release of these cyanobacteria toxins directly harms fish, humans and animals. Cyanobacteria toxins can be divided into four categories according to the different diseases that they ultimately cause: neurotoxins, liver toxins, cytotoxins and irritant toxins. Among the four types of toxins, the hepatic toxin, microcystin, is the most harmful to the public's health, and the serious one can cause its death. Therefore, scientists from various countrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 朱鹏严小军严婷婷陈海敏游玉容
Owner NINGBO UNIV
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