47 results about "Synthetic gene" patented technology

Methods for making synthetic gene clusters are described.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

The invention discloses a sequence optimization method for improving the stability of circular RNA, application and a product, and belongs to the technical field of biotechnology and molecular biology. In order to solve the problem that the existing circular RNA is easy to be specifically degraded by RNAseK and lysosome after entering a cell, synonymous mutation optimization is carried out on an open reading frame of a target gene of the circular RNA by identifying nucleic acid motifs (a first type of nucleic acid motifs and a second type of nucleic acid motifs) identified by two degradation mechanisms. The optimized circular RNA is prepared by means of in-vitro cyclization of an anabaena I-type intron self-splicing mediator, after cells are transfected, the intracellular RNA expression level of the circular RNA is remarkably improved compared with that of a wild type, and the target protein expression efficiency is also remarkably improved. The intracellular stability and expression efficiency of the circular RNA are remarkably enhanced, and the method can be widely applied to the scenes of gene expression regulation, protein synthesis, gene therapy, vaccine development and the like.

Various devices, systems, structures and methods are disclosed related to securely authorizing a transaction by synchronizing digital genomic data with associated synthetic genomic variants. An embodiment of the present invention utilizes digital genomic data associated with an entity, such as a person, who may utilize a genome-based security device to complete a transaction. In one embodiment, a person may use a genome-based security device to communicate with an external device over a wireless or other communication interface, synchronize digital genomic data and an associated synthetic variant received from the external device with digital genomic data and associated synthetic variant stored on the genome-based security device.

The invention belongs to the technical field of lipopeptide preparation, genetic engineering and biological medicine, and particularly relates to novel double-fat-chain lipopeptide as well as a preparation method and application thereof. According to the invention, a novel artificial gene cluster rhc is successfully constructed through precise editing and recombination of a functional module of a natural non-ribosomal lipopeptide gene cluster, and after heterologous expression of the gene cluster, a brand new double-fat-chain lipopeptide product Rhcolide A can be synthesized; the rhc gene cluster used for preparing the novel double-fat-chain lipopeptide Rhcolide A is the first artificial double-fat-chain lipopeptide synthetic gene cluster constructed through genetic engineering modification. A biological function test verifies and finds that Rhcolide A has excellent ferroptosis sensitizing activity, can be used as a ferroptosis sensitizer to be combined with a ferroptosis inducer to prepare a non-small cell carcinoma resisting drug, provides a brand new drug molecular entity for treatment of non-small cell carcinoma, and has important clinical application value and industrialization prospect.

The application discloses a method for biosynthesis of gene or gene cluster product based on chromatin three-dimensional structure, and belongs to the field of genetic engineering. The method can realize the improvement of the target gene or gene cluster product by the directional integration of the target gene or gene cluster into the strong correlation chromatin region with high frequency interaction on the genome. The method has the characteristics of simple implementation, good repeatability, remarkable effect and the like, and can be widely applied to the improvement of the target gene or gene cluster product.

Disclosed herein are shelf-stable compositions based on synthetic gene networks and / or cell-free systems that are lyophilized on a solid support. The compositions can be easily transported and stored for a period of time, and activation can be done by simply adding water. Methods of use are also disclosed herein, including, but are not limited to, sensing and a variety of logic functions. The invention permits straightforward, sterile and abiotic distribution of synthetic biology-based technology to clinical settings, food processing and industry, the military and consumer products.

The present invention is related to the recombinant production of proteins by using a synthetic gene for high protein expression in Pichia pastoris. More specifically, the invention describes the production of Sm14 Schistosoma mansoni recombinant protein, where a synthetic gene was created to promote high expression of such protein, a gene which was cloned under control of two types of Pichia pastoris promoters: methanol-inducible promoter (AOXI) and constituent promoter (GAP). With these constructions, Pichia pastoris strains were genetically manipulated to efficiently produce vaccine antigen Sm14. The processes to produce and purify this protein from P. pastoris cells, which can be escalated for their industrial production, were also improved.

The invention provides application of a transcription factor PgWRKY2 gene in promotion of punicalagin synthesis, and belongs to the technical field of gene engineering and molecular biology, experiments verify that the PgWRKY2 gene can positively regulate and control expression of punicalagin synthesis genes PgUGT84A23 and PgPOR9, so that synthesis of punicalagin is promoted; the silent PgWRKY2 in pomegranate peel can inhibit the expression of PgUGT84A23 and PgPOR9, so that the content of punicalagin is reduced. The invention provides an important theoretical basis for improving the pomegranate quality and cultivating excellent pomegranate varieties.

This invention describes artificial expression constructs for regulating gene expression in target types of central nervous system cells. By using the artificial expression constructs of this disclosure, it is possible to express or modify the expression of synthetic genes in spinal motor neurons, including SpP1 spinal motor neurons, Parg spinal motor neurons, Ogdh1 spinal motor neurons, and ChAT spinal motor neurons; α motor neurons, including Chodl spinal motor neurons; γ motor neurons; spinal excitatory motor neurons, including Mafa excitatory neurons, Esrrg Trhr excitatory neurons, and Slc17a6 spinal excitatory neurons; spinal inhibitory neurons, including Slc6a5 spinal inhibitory neurons; whole spinal neurons, including Esrrg spinal motor neurons and types of neurons found throughout the spinal cord; cerebrospinal fluid contact neurons, including Poln spinal motor neurons; and non-neuronal cells of the spinal cord, including astrocytes and oligodendrocytes.

The present application relates to a tetracyclic sesquiterpene compound and a synthetic gene cluster thereof, and belongs to the field of genetic engineering. The tetracyclic sesquiterpene compound synthetic gene cluster is derived from Fusarium oxysporum 14005, and the gene cluster contains six genes, namely, a gene fomdE or a functional equivalent thereof encoding a sesquiterpene synthase FoMS, genes fomdA, fomdC and fomdD or functional equivalents thereof encoding cytochrome P450 enzymes fomdA, fomdC and fomdD, a gene fomdB or a functional equivalent thereof encoding an aldehyde-ketone reductase fomdB, and a gene fomdF or a functional equivalent thereof encoding a hydrolase fomdF. The Mangicols gene cluster discovered in the present application catalyzes the production of a new tetracyclic sesquiterpene compound, and provides valuable lead compound resources for enriching the natural product compound library and discovering new antibiotics.

The invention provides a preparation method of an animal and plant fusion exosome loaded with siRNA, and the method comprises the following steps: uniformly mixing a purified honeysuckle plant exosome and a purified brain glioma exosome, adding polyvinyl alcohol, and repeatedly freezing and thawing to obtain the animal and plant fusion exosome; siRNA of the IDO gene is synthesized, and siRNA is obtained; and co-incubating and culturing the obtained animal and plant fusion exosome and the obtained siRNA, and purifying to obtain the purified animal and plant fusion exosome loaded with siRNA. The invention also provides an application of the animal and plant fusion exosome loaded with siRNA, wherein the animal and plant fusion exosome is used for preparing a drug for resisting brain glioma. The animal and plant fusion exosome loaded with the siRNA prepared by the invention has good biocompatibility, low immunogenicity and efficient cell uptake ability, and the exosome can effectively protect the siRNA from being degraded and accurately deliver the siRNA to a target cell; the antigen presentation function of the dendritic cells is enhanced, the T cell reaction is more effectively activated, and the T cell depletion is reversed, so that the anti-glioma immune response is improved.

The application discloses a xanthostemane synthetic gene, an encoded protein and application thereof. The gene is cloned from xanthostemane, a nucleotide sequence of which is shown in bases 1-1578 of SEQ ID NO. 1, and an amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The gene is overexpressed in xanthostemane hairy roots, and can promote the increase of xanthostemane content. Therefore, the xanthostemane gene has important application value in promoting xanthostemane germplasm improvement, variety optimization and industrial development.

The invention discloses a monascus MpigI gene interference strain as well as a construction method and application thereof, and relates to the technical field of molecular biology, the monascus MpigI gene interference strain is preserved in China Center for Type Culture Collection on November 20, 2025, and the preservation number is CCTCC NO: M 20252614. According to the present invention, the influence of the transcriptional regulation factor in the MPs synthesis gene cluster on the secondary metabolism of fungi is utilized to construct the monascus ruber Ii4 interfering the monascus ruber, the preservation number of the monascus ruber Ii4 is CCTCC NO: M 20252614, and compared with the initial strain monascus ruber M7 (CCAM 070120), the mutant strain can secrete a large amount of monascus ruber, orange pigment and yellow pigment, the intracellular haematochrome yield increase speed of the mutant strain of the red monascus Ii4 is fastest, and is about 3-5 times of the intracellular haematochrome yield of M7.

Materials and methods for labeling and isolating particular cell types from mixed cell populations are provided herein. Also provided herein are methods for generating data representing a synthetic genetic sequence configured for labeling at least one cell type by causing expression of a marker in the at least one cell type.

The invention relates to the technical field of microorganisms, and particularly discloses lysinibacillus fusiformis KBD-5 for antagonizing botrytis cinerea and application of the lysinibacillus fusiformis KBD-5. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) Whole genome (accession number PRJNA1335659) sequencing reveals that the strain has a triple synergistic antagonistic mechanism for the first time: the strain contains a fengycin-like lipopeptide synthetic gene cluster to destroy pathogenic bacteria cell membranes, and contains GH18 chitinase and GH5 cellulase genes to degrade pathogenic bacteria cell walls. The inhibition rate of the strain on botrytis cinerea reaches 76.42%; the tomato fruit test morbidity is reduced to 25.9%, and the rotting index is reduced by 75.0%; the control effect of greenhouse potting reaches 72.35%, and the control effect is not obviously different from that of procymidone. Biological safety assessment shows that no toxicity factor exists, and no drug-resistant gene horizontal transfer risk exists. The invention further provides a microbial preparation containing the KBD-5 and application of the microbial preparation.

The application relates to the technical field of animal model construction, and particularly discloses a Sypl1 gene knockout colorectal cancer mouse model construction method and application, which comprises the following steps: synthesizing a specific target site gRNA of a Sypl1 gene, mixing Cas9 protein and the target site gRNA to obtain an injection compound, microinjecting the injection compound into mouse zygotes, and transplanting the surviving zygotes into the oviducts of pseudopregnant female mice to obtain F0 generation mice, and the mice born after 20 days are Sypl1 gene knockout mouse animal models; and a chemical induction modeling method is used to construct a colorectal cancer model in wild type mice and gene knockout mice. The Sypl1 gene knockout mouse is constructed by using a CRISPR / Cas mediated genome engineering technology Sypl1 combined with the most widely used colorectal cancer chemical inducer azoxymethane / dextran sodium sulfate, a new colorectal cancer mouse model is constructed, compared with a traditional chemical induction model, the tumor formation rate can be significantly improved, and the tumor formation time can be shortened, and a more efficient animal model is provided for colorectal cancer research.

Artificial expression constructs are provided.SOLUTION: Artificial expression constructs are provided that selectively regulate gene expression in selected central nervous system cell types. Such artificial expression constructs can be used to selectively express synthetic genes or alter the expression of genes in inhibitory neocortical GABAergic neurons, including somatostatin GABAergic neurons, parvalmine GABAergic neurons, vasoactive intestinal peptide GABAergic neurons, Lamp5GABA GABAergic neurons, or in some instances astrocytes.SELECTED DRAWING: Figure 2-1

The invention discloses an abelmoschus manihot flavonoid compound synthetic gene AmbHLH as well as an encoding protein and application thereof. The transcription factor AmbHLH is cloned from abelmoschus manihot, the nucleotide sequence of the transcription factor AmbHLH is shown as SEQ ID NO.1, and the amino acid sequence coded by the transcription factor AmbHLH is shown as SEQ ID NO.2. The gene AmbHLH is over-expressed in tobacco, and the increase of the total flavonoid content of the tobacco can be promoted. The gene AmbHLH is over-expressed in the abelmoschus manihot, and the increase of the total flavone content and quercetin of the abelmoschus manihot can be promoted. Therefore, the abelmoschus manihot AmbHLH gene provided by the invention has important theoretical and application values in the aspect of regulating and controlling the synthesis of flavonoid compounds.

The present invention relates to a rapid, high-fidelity process to support synthesis of genes for in vitro transcription of modified messenger RNA. In this process, sequence errors resulting from amplification with oligonucleotides comprising inherent errors are significantly reduced.

Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in forebrain glutamatergic neurons.

The invention relates to the technical field of animal model construction, and particularly discloses a construction method and application of a Sypl1 gene knockout colorectal cancer mouse model, and the construction method comprises the following steps: synthesizing a specific target site gRNA of a Sypl1 gene, uniformly mixing Cas9 protein and the target site gRNA to obtain an injection compound, microinjecting the injection compound into a mouse fertilized egg to obtain a Sypl1 gene knockout colorectal cancer mouse model. And transplanting the survival fertilized ova into the salpingtube of a pseudopregnant female mouse for 20 days to obtain a mouse which is an F0-generation mouse, obtaining a Sypl1 gene knockout mouse animal model, and constructing a colorectal cancer model in a wild type mouse and a gene knockout mouse by adopting a chemical induction modeling method. A Sypl1 gene knockout mouse is constructed through a CRISPR / Cas mediated genome engineering technology, a novel colorectal cancer mouse model is constructed in combination with a colorectal cancer chemical inducer azomethane / dextran sodium sulfate which is most widely applied at present, and compared with a traditional chemical induction model, the novel colorectal cancer mouse model has the advantages that the tumor formation rate can be remarkably increased, the tumor formation time can be shortened, and the tumor formation cost can be reduced. And a more efficient animal model is provided for colorectal cancer research.

The invention relates to the technical field of genetic engineering, in particular to a promoter and engineering bacteria capable of increasing the yield of butenyl spinosad. According to the invention, an efficient constitutive promoter adaptive to saccharopolyspora tuberosa is obtained by utilizing random mutation, and the promoter greatly improves the intracellular transcriptional level of a synthetic gene related to butenyl spinosad, so that the yield of fermentation production of butenyl spinosad is improved. The invention not only can provide a core regulatory element for the yield increase of the secondary metabolite of the saccharopolyspora tuberosa, but also can promote the technical upgrade of the synthetic biological transformation of the saccharopolyspora tuberosa, and has important theoretical significance and application value.

The present application relates to the technical field of genetic engineering, and particularly relates to a promoter and an engineering bacterium capable of improving the yield of butenyl polyoxin. The present application obtains a high-efficiency constitutive promoter of Sacccharopolyspora gilva by random mutation. The promoter greatly improves the intracellular transcription level of butenyl polyoxin related synthesis genes, thereby improving the yield of butenyl polyoxin in fermentation production. The present application not only provides a core regulatory element for improving the yield of secondary metabolites of Sacccharopolyspora gilva, but also promotes the technical upgrading of synthetic biology modification, and has important theoretical significance and application value.

The invention belongs to the field of synthetic biology and microbial pharmacy, and particularly relates to recombinant streptomyces, a construction method of the recombinant streptomyces and application of the recombinant streptomyces in preparation of savermectin B. According to the present invention, after the aveA1 gene segment in the abamectin biosynthesis gene cluster in the streptomyces avermectin is replaced with the nemA1 gene segment in the nemadectin biosynthesis gene cluster in the streptomyces blue griseolus, the recombinant streptomyces capable of producing the 16-membered macrolide compound-Sesvermectin B (Senvermectin B), such as the recombinant streptomyces avermectin HU520-M provided by the invention, is obtained; experiments show that the savermectin B has efficient insecticidal, acaricidal and anti-parasitic activity, and shows good industrial development potential.

To provide an artificial expression construct for selectively regulating gene expression.SOLUTION: There is provided an artificial expression construct comprising (i) a concatemer comprising a specific sequence, (ii) a promoter, and (iii) a heterologous coding sequence, for selectively modulating gene expression in a selected central nervous system cell type. The artificial expression construct can be used to selectively express the synthetic gene or modulate gene expression, particularly in cortical excitatory neurons, including those predominantly within cortical layers 2 / 3, 4, 5, and 6, and having extracerebral (ET), intracerebral (IT), and pyramidal tract (PT) projections.SELECTED DRAWING: None

The invention provides an amplification library building method for high-throughput sequencing verification of synthetic genes. The amplification library building method comprises the following steps: obtaining a joint connection product of which two ends are connected with double-chain joints after a to-be-detected synthetic gene sequence, and carrying out first PCR (Polymerase Chain Reaction) amplification by adopting a first gene specific primer by taking the joint connection product as a template to obtain a first amplification product; taking the first amplification product as a template, and carrying out second PCR amplification by adopting a second gene specific primer to obtain a second amplification product; performing thermal denaturation single-chain separation and single-chain cyclization on the second amplification product to obtain single-chain circular DNA; the preparation method comprises the following steps: carrying out DNB preparation on single-stranded circular DNA to obtain DNB nanospheres; and sequencing the DNB nanospheres to obtain synthetic gene sequence information. The method verifies that the synthesized gene is high in flux, and the flux requirement of large-scale gene synthesis can be met.

This invention belongs to the field of synthetic biology and discloses tetracycline compounds modified with a polysaccharide group at the C8 position, their biosynthetic methods, and applications. The tetracycline compounds with a polysaccharide group modification at the C8 position provided by this invention have a novel structure; the trisaccharide and hexasaccharide groups present at the C8 position are not found in known tetracycline natural products or tetracycline skeleton antibiotics. The biosynthetic method involves using a polysaccharide group derived from *Streptomyces rubrum*. ech Gene clusters, in which mis This invention expresses the gene cluster in the *Streptomyces micranthum* host, fully utilizing the different biosynthetic enzymes contained in two similar but different tetracycline natural product biosynthetic gene clusters to synthesize novel compounds that neither host bacterium can synthesize. These compounds exhibit significant inhibitory activity against *Staphylococcus aureus*, *Enterococcus faecalis*, and *Enterococcus faecium*. This invention provides new candidate compounds for the innovative development of tetracycline-based antibacterial drugs and is of great significance to the development of microbial drug resources.

The present invention provides a synthetic, modular system for DNA modification as may be employed for genome editing comprising a targeting nucleic acid possessing both DNA targeting ability and ability to bind a recognition module of a modular polypeptide component, where the modular polypeptide component also includes an effector component and a short peptide DNA-binding sequence (DBD) which binds a pre-determined sequence (PBS) in the target. The DBD may preferably be no more than a 15 mer and serves to destabilize the structure of a targeted dsDNA upon binding thereby facilitating the desired DNA modification. The effector component may, for example, be an artificial nickase comprising linked self-assembling peptides thereby providing a complete genome-editing system of advantageous small size for vector delivery to cells.