90 results about "Synthetic gene" patented technology

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

Disclosed in the present invention is a use of genes NAT1 and bHLH110 in improving high-temperature resistance of rice, for use in using gene editing technology to obtain a rice material having enhanced high-temperature resistance. The base sequence of a rice gene NAT1 is as shown in SEQ ID NO. 1, and the base sequence of a gene bHLH110 is as shown in SEQ ID NO. 4. A base of a rice gene NAT1 of rice is replaced, deleted or inserted through gene editing technology to release inhibition on the rice gene bHLH110, and the expression of wax synthesis genes CER1 and CER1L is promoted by the rice gene bHLH110 to enhance the high-temperature resistance of a plant, thereby preparing a rice material having enhanced high-temperature resistance. According to the present invention, a NAT1 mutant rice material Nipponbare obtained by using CRISPR-Cas9 gene editing technology has remarkably enhanced high-temperature resistance, including an improved survival rate in a seedling stage and improved agronomic traits in a reproductive stage.

Methods for making synthetic gene clusters are described.

Aspects of the invention is directed to a high-throughput method for screening a gene circuit library. The pace of gene circuit engineering can thereby be increased by expanding the number of circuits tested in each cycle. By measuring large collections of circuits in a single experiment, such an approach could be used for rapidly profiling complex circuit design spaces to identify part compositions with desired quantitative behaviors.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The invention provides an in-vitro transcription method based on RNA polymerase and a kit, which are suitable for the fields of mRNA synthesis, gene research and biological pharmacy. According to the method, the problems of low efficiency, poor stability and the like of traditional in-vitro transcription are solved through an optimization technology. The method comprises the following specific steps: providing a linear DNA template containing a T7 promoter; according to the method, site-directed mutagenesis or fusion protein modified high-activity T7RNA polymerase is combined with chemically modified NTPs and a spermine-BSA compound stabilizer to react under the conditions that the temperature is 37-42 DEG C and the pH is 7.5-8.5, and formation of double-stranded RNA is promoted through dynamic temperature control. A reaction system contains Mg < 2 + > / Mn < 2 + > and DTT, and enzyme activity and product integrity are optimized. The kit comprises an improved reagent and a standardized process, the transcription efficiency is improved by 2-5 times, the product yield is greater than or equal to 80%, and the activity is not lost after the kit is stored at 4 DEG C for 1 month. The method has the technical advantages that NTPs degradation resistance is achieved, template preparation is simplified, universality is high, the method is suitable for large-scale production of mRNA vaccines, gene therapy and the like, the production cost is remarkably reduced, and wide application of the in-vitro transcription technology is promoted.

Methods for identifying biosynthetic gene clusters that include genes for producing compounds that interact with specific target proteins are disclosed. Some methods relate to bioinformatics methods for identifying and / or prioritizing biosynthetic gene clusters. Related systems, components, and tools for the identification and expression of such gene clusters are also disclosed.

This invention belongs to the field of tobacco genetic engineering, specifically relating to the application of the ferulic acid synthesis gene NtKAT2.2 in tobacco. The tobacco ferulic acid synthesis gene... NtKAT2.2 The nucleotide sequence is shown in SEQ ID No. 1. NtKAT2.2 Gene expression levels are negatively correlated with ferulic acid content in tobacco. Preliminary research results indicate that the gene involved in this application... NtKAT2.2 The gene is highly correlated with ferulic acid content in tobacco. Silencing this gene significantly increases ferulic acid content in the resulting tobacco lines. Based on this result, the inventors believe that further in-depth research on this gene could lay a solid technical foundation for improving tobacco quality and developing new varieties.

The invention discloses a honeysuckle flower pigment synthesis gene and application thereof in regulating and controlling the content and resistance of plant anthocyanidin, and belongs to the technical field of gene engineering. The nucleotide sequence of the honeysuckle flower pigment synthesis gene is as shown in SEQ ID NO. 6. The invention provides a honeysuckle flower pigment synthesis gene. The gene has the effects of regulating and controlling the content of anthocyanidin in plants and regulating and controlling the stress resistance of the plants. The gene is introduced into a plant body by constructing an overexpression vector, so that the gene is stably overexpressed, the anthocyanidin content in the plant is remarkably increased, and the petal color and seed coat color of the plant are darkened; the resistance of the plant to drought stress and salt stress is also remarkably enhanced, so that the plant can adapt to a severe environment more easily, and a new gene resource and a theoretical support are provided for breeding of high-resistance plant varieties.

Methods, systems and apparatus are described for generating synthetic gene expression profile datasets for downstream biological / pharmacological analysis, processing and / or applications Generating synthetic gene expression profile datasets includes: receiving a gene expression profile dataset comprising data representative of at least one gene expression profile; computing a complex gene expression profile dataset based on applying a discrete Fourier transform to data representative of each gene expression profile of the received gene expression profile dataset; sampling the complex gene expression profile dataset based on using a statistical distribution to sample and modify a predetermined number of one or more components of each complex gene expression profile for generating a plurality of synthetic complex gene expression profiles; computing a real-valued synthetic gene expression profile dataset based on applying an inverse discrete Fourier transform to the synthetic complex gene expression profile dataset; and outputting data representative of the real-valued synthetic gene expression profile dataset for downstream biological / pharmacological analysis, processing and / or applications.

The invention belongs to the technical field of biology, and particularly relates to a genetically engineered bacterium for producing a heparin precursor and a construction method and application of the genetically engineered bacterium. According to the technical scheme adopted by the invention, a lipopolysaccharide (LPS) synthetic gene waaJ and a claic acid synthetic gene cluster wca are knocked out, and the synthesis of LPS and CA is blocked, so that more carbon sources enter a heparin precursor synthetic route, and the yield and / or conversion rate of the heparin precursor are / is improved. According to the invention, heparin precursor synthetases KfiA (UDP-GlcNAc glycosyl transferase), KfiC (UDP-GlcA glycosyl transferase) and KfiD (UDP-glucose dehydrogenase) are further enhanced, the expression of UDP-GlcA generated by UDP-Glc is catalyzed, and the yield and / or conversion rate of the heparin precursor can be further improved.

The invention discloses a sequence optimization method for improving the stability of circular RNA, application and a product, and belongs to the technical field of biotechnology and molecular biology. In order to solve the problem that the existing circular RNA is easy to be specifically degraded by RNAseK and lysosome after entering a cell, synonymous mutation optimization is carried out on an open reading frame of a target gene of the circular RNA by identifying nucleic acid motifs (a first type of nucleic acid motifs and a second type of nucleic acid motifs) identified by two degradation mechanisms. The optimized circular RNA is prepared by means of in-vitro cyclization of an anabaena I-type intron self-splicing mediator, after cells are transfected, the intracellular RNA expression level of the circular RNA is remarkably improved compared with that of a wild type, and the target protein expression efficiency is also remarkably improved. The intracellular stability and expression efficiency of the circular RNA are remarkably enhanced, and the method can be widely applied to the scenes of gene expression regulation, protein synthesis, gene therapy, vaccine development and the like.

The present application relates to a kind of cyclic ester peptide compound aglomycin A biosynthesis gene cluster and its application.The nucleotide sequence of the gene cluster is as shown in SEQ ID NO.1, and the use is to produce peptide compound aglomycin A or peptide compound aglomycin D.

Various devices, systems, structures and methods are disclosed related to securely authorizing a transaction by synchronizing digital genomic data with associated synthetic genomic variants. An embodiment of the present invention utilizes digital genomic data associated with an entity, such as a person, who may utilize a genome-based security device to complete a transaction. In one embodiment, a person may use a genome-based security device to communicate with an external device over a wireless or other communication interface, synchronize digital genomic data and an associated synthetic variant received from the external device with digital genomic data and associated synthetic variant stored on the genome-based security device.

The invention belongs to the technical field of lipopeptide preparation, genetic engineering and biological medicine, and particularly relates to novel double-fat-chain lipopeptide as well as a preparation method and application thereof. According to the invention, a novel artificial gene cluster rhc is successfully constructed through precise editing and recombination of a functional module of a natural non-ribosomal lipopeptide gene cluster, and after heterologous expression of the gene cluster, a brand new double-fat-chain lipopeptide product Rhcolide A can be synthesized; the rhc gene cluster used for preparing the novel double-fat-chain lipopeptide Rhcolide A is the first artificial double-fat-chain lipopeptide synthetic gene cluster constructed through genetic engineering modification. A biological function test verifies and finds that Rhcolide A has excellent ferroptosis sensitizing activity, can be used as a ferroptosis sensitizer to be combined with a ferroptosis inducer to prepare a non-small cell carcinoma resisting drug, provides a brand new drug molecular entity for treatment of non-small cell carcinoma, and has important clinical application value and industrialization prospect.

The application discloses a method for biosynthesis of gene or gene cluster product based on chromatin three-dimensional structure, and belongs to the field of genetic engineering. The method can realize the improvement of the target gene or gene cluster product by the directional integration of the target gene or gene cluster into the strong correlation chromatin region with high frequency interaction on the genome. The method has the characteristics of simple implementation, good repeatability, remarkable effect and the like, and can be widely applied to the improvement of the target gene or gene cluster product.

Disclosed herein are shelf-stable compositions based on synthetic gene networks and / or cell-free systems that are lyophilized on a solid support. The compositions can be easily transported and stored for a period of time, and activation can be done by simply adding water. Methods of use are also disclosed herein, including, but are not limited to, sensing and a variety of logic functions. The invention permits straightforward, sterile and abiotic distribution of synthetic biology-based technology to clinical settings, food processing and industry, the military and consumer products.

The invention discloses a bacillus velezensis ATC-AL and application thereof. The preservation number of the strain is CCTCC (China Center For Type Culture Collection): M20251280. A genome of the bacillus velezensis ATC-AL has a synthetic gene cluster for synthesizing various antifungal active substances, the active substances of the bacillus velezensis ATC-AL can destroy cell surface structures of pathogenic bacteria, induce cell apoptosis and inhibit spore development of pathogenic fungi, and the bacillus velezensis ATC-AL has a broad-spectrum inhibition effect on various pathogenic fungi such as sweet potato coracocystis canaliculata, fusarium graminearum and aspergillus flavus; the generation inhibition rate of vomitoxin reaches 95%, and the generation inhibition rate of aflatoxin B1 can reach 100%; fungus infection experiments on sweet potatoes, corn and wheat prove that active substances in a strain cell fermentation broth can completely inhibit the decay effect of sweet potato coracocystis longirostris, fusarium graminearum and aspergillus flavus on the food crops; according to the invention, a microbial control resource library for biological control of pathogenic fungi and toxin pollution thereof is enriched.

The present invention relates to a synthetic gene cluster of an enfumafungin antibiotic and a synthetic method therefor. Specifically, in the present invention, key functional genes in the linking of a β-D-glucopyranose at position C3 of a fernane-type framework, the oxidation at position C2 into α-OH, the oxidative cleavage of ring E at C19-C20, and the acetylation of hydroxyl at position C2 during the biosynthesis of an enfumafungin antibiotic, i.e. fuscoatroside, are isolated, wherein the genes are named fsoA, fsoD, fsoE and fsoF, respectively. The present invention also provides encoding polypeptides thereof. Provided in the present invention are an artificial fusion enzyme gene, i.e. efuA(TC)fsoA(GT); and on the basis of the artificial fusion enzyme gene, the heterologous expression of four genes, i.e. efuA(TC)fsoA(GT), fsoD, fsoE and fsoF, can synthesize an enfumafungin precursor (13). The present invention clarifies an FsoE-mediated C-C bond breaking function of a P450 enzyme, and provides a key catalytically active residue of FsoE. The present invention reports the biosynthetic pathway of such compound for the first time, and establishes an important foundation for the green and efficient synthesis of the compound.

The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and / or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and / or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

The present invention is related to the recombinant production of proteins by using a synthetic gene for high protein expression in Pichia pastoris. More specifically, the invention describes the production of Sm14 Schistosoma mansoni recombinant protein, where a synthetic gene was created to promote high expression of such protein, a gene which was cloned under control of two types of Pichia pastoris promoters: methanol-inducible promoter (AOXI) and constituent promoter (GAP). With these constructions, Pichia pastoris strains were genetically manipulated to efficiently produce vaccine antigen Sm14. The processes to produce and purify this protein from P. pastoris cells, which can be escalated for their industrial production, were also improved.

The invention provides application of a transcription factor PgWRKY2 gene in promotion of punicalagin synthesis, and belongs to the technical field of gene engineering and molecular biology, experiments verify that the PgWRKY2 gene can positively regulate and control expression of punicalagin synthesis genes PgUGT84A23 and PgPOR9, so that synthesis of punicalagin is promoted; the silent PgWRKY2 in pomegranate peel can inhibit the expression of PgUGT84A23 and PgPOR9, so that the content of punicalagin is reduced. The invention provides an important theoretical basis for improving the pomegranate quality and cultivating excellent pomegranate varieties.

This invention describes artificial expression constructs for regulating gene expression in target types of central nervous system cells. By using the artificial expression constructs of this disclosure, it is possible to express or modify the expression of synthetic genes in spinal motor neurons, including SpP1 spinal motor neurons, Parg spinal motor neurons, Ogdh1 spinal motor neurons, and ChAT spinal motor neurons; α motor neurons, including Chodl spinal motor neurons; γ motor neurons; spinal excitatory motor neurons, including Mafa excitatory neurons, Esrrg Trhr excitatory neurons, and Slc17a6 spinal excitatory neurons; spinal inhibitory neurons, including Slc6a5 spinal inhibitory neurons; whole spinal neurons, including Esrrg spinal motor neurons and types of neurons found throughout the spinal cord; cerebrospinal fluid contact neurons, including Poln spinal motor neurons; and non-neuronal cells of the spinal cord, including astrocytes and oligodendrocytes.

The present application relates to a tetracyclic sesquiterpene compound and a synthetic gene cluster thereof, and belongs to the field of genetic engineering. The tetracyclic sesquiterpene compound synthetic gene cluster is derived from Fusarium oxysporum 14005, and the gene cluster contains six genes, namely, a gene fomdE or a functional equivalent thereof encoding a sesquiterpene synthase FoMS, genes fomdA, fomdC and fomdD or functional equivalents thereof encoding cytochrome P450 enzymes fomdA, fomdC and fomdD, a gene fomdB or a functional equivalent thereof encoding an aldehyde-ketone reductase fomdB, and a gene fomdF or a functional equivalent thereof encoding a hydrolase fomdF. The Mangicols gene cluster discovered in the present application catalyzes the production of a new tetracyclic sesquiterpene compound, and provides valuable lead compound resources for enriching the natural product compound library and discovering new antibiotics.

The patent belongs to the field of plant biotechnology, and particularly relates to a nucleic acid aptamer with high binding force specificity to Arabidopsis COP1 protein and a preparation method. To solve the time-consuming and laborious problem of existing screening technology, the patent provides a nucleic acid aptamer with specificity to Arabidopsis COP1 protein and a preparation method. The nucleic acid aptamer Lib2-11 of Arabidopsis COP1 is obtained through One-step screening technology, the nucleic acid aptamer has high binding and high specificity, and after optimization of the sequence, the nucleic acid aptamer Lib2-11c with high binding performance is obtained. The nucleic acid aptamer Lib2-11 and Lib2-11c of the patent have important application prospects for determining the Arabidopsis COP1 protein level, subcellular localization and light-controlled synthetic gene circuit.

The invention relates to a physical property improvement method, extraction method and application of poplar nanocellulose, the improvement method is to improve the physical property of the poplar nanocellulose by inhibiting the expression of lignin synthetic gene CSE, and the specific improvement is that the biomass is slightly reduced, the lignin content is reduced, and the crystallinity of the cellulose is slightly increased. And the length-diameter ratio of the nanocellulose is reduced, so that the mechanical property of the nanocellulose is enhanced. The extraction method comprises the following steps: preparing raw materials; removing lignin by using sodium chlorite; carrying out TEMPO (Tetramethylpiperidinol) mediated oxidation treatment; and defibrating and dispersing the nanofibers. The improved nanocellulose is applied to the field of nanofiber-based materials, particularly to preparation of aerogel and films, the compressive strength and elasticity of the prepared aerogel are enhanced, the ductility, tensile strength and breaking energy of the films are higher, and the material toughness is better. The invention provides new breakthrough and inspiration for the application of the nanocellulose, and clarifies the value of the CSE gene in the aspects of wood quality character improvement and nanofiber-based application.

1. The name of this design product: Multi-channel synthesis module. 2. Purpose of the product of this design: for use in a multi-channel column enzymatic synthesizer for synthesizing gene sequences. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention provides a preparation method of an animal and plant fusion exosome loaded with siRNA, and the method comprises the following steps: uniformly mixing a purified honeysuckle plant exosome and a purified brain glioma exosome, adding polyvinyl alcohol, and repeatedly freezing and thawing to obtain the animal and plant fusion exosome; siRNA of the IDO gene is synthesized, and siRNA is obtained; and co-incubating and culturing the obtained animal and plant fusion exosome and the obtained siRNA, and purifying to obtain the purified animal and plant fusion exosome loaded with siRNA. The invention also provides an application of the animal and plant fusion exosome loaded with siRNA, wherein the animal and plant fusion exosome is used for preparing a drug for resisting brain glioma. The animal and plant fusion exosome loaded with the siRNA prepared by the invention has good biocompatibility, low immunogenicity and efficient cell uptake ability, and the exosome can effectively protect the siRNA from being degraded and accurately deliver the siRNA to a target cell; the antigen presentation function of the dendritic cells is enhanced, the T cell reaction is more effectively activated, and the T cell depletion is reversed, so that the anti-glioma immune response is improved.

In accordance with some embodiments, systems, methods, and media for classifying genetic sequencing results are provided. In some embodiments, a system includes a processor programmed to: receive a sample genetic sequencing result for a reference organism and for a host organism, generate a plurality of synthetic genetic sequencing results by combining a portion of the sample genetic sequencing result for the reference organism and the host organism, generate a matrix by cross-referencing a pair of synthetic genetic sequencing results, generate a model based on the synthetic genetic sequencing results, determine at least one threshold based on the matrix, update the model based on the threshold, receive a clinical sample genetic sequencing result, identify, utilizing the model, any values in the clinical sample genetic sequencing result that are likely to be diagnostically significant; generate a report; and cause the report to be presented to a user.