205 results about "Synthetic gene" patented technology

Methods for making synthetic gene clusters are described.

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention discloses a DNA synthesizer, and relates to the technical field of biology. The DNA synthesizer comprises a shell assembly, a mobile module, a synthesis unit and a separate injection unit, the mobile module is mounted in the shell assembly; the synthesis unit comprises a bearing part and a synthesis plate arranged on the bearing part, the bearing part is arranged on the moving module, a synthesis cavity is defined by the bearing part and the shell assembly, a protective gas inlet and a protective gas outlet are formed in the bearing part, and a sealing structure which is in sliding friction with the inner wall of the shell assembly is arranged on the bearing part; an adjusting mechanism used for driving the sealing structure to be away from or close to the shell assembly is arranged on the bearing part, and the moving module is used for driving the synthesis unit to move in at least one direction; the dispensing unit is arranged on the shell assembly and is used for injecting a reagent into the synthesis plate; the structure of the DNA synthesizer is improved, multiple types of genes can be synthesized, the application is wide, the whole structure is compact, the synthesis cavity is small in size, the loss of synthesis environment protection gas is effectively reduced, and the synthesis cost is remarkably reduced.

The invention discloses an application of a populus tomentosa IPT5b gene in creating a high-yield shade-tolerant populus germplasm, belongs to forest breeding, and is characterized in that a phloem specific promoter CLE41b is utilized to express a cytokinin synthetic gene IPT5b in populus tomentosa, and an overexpression material of which cambium activity and xylem development are remarkably promoted is created. After shading treatment, the number of cambium cell layers and the number of xylem cell layers of CLE41bpro: IPT5b are remarkably higher than those of wild type CLE41bpro: IPT5b, and the phenotype increases the content of cytokinin (CK) in stems, so that the wood yield of trees under the shading condition can be increased. According to the method, a high-yield and shade-tolerant new poplar variety is created in a manner of finely regulating and controlling the content of CK in the phloem of the poplar. The gene source and scientific reference are provided for improving and improving the yield and shade tolerance of other trees, and the gene has important application value in the field of forest tree gene engineering.

The invention discloses a medicine for removing SHBs / HBsAg protein and a preparation method thereof, and relates to the technical field of biology, the medicine comprises a recombinant plasmid of a TRIM21 gene and / or OTUD4 small interfering RNA; the preparation method comprises the following steps: synthesizing a TRIM21 gene and cloning the TRIM21 gene to an expression vector to obtain a recombinant plasmid; synthesizing a small interfering RNA (Ribonucleic Acid) of the OTUD4 gene; and transfecting the constructed recombinant plasmid or the synthesized small interfering RNA into liver cancer cells for expressing SHBs protein or HBV (Hepatitis B Virus). According to the present invention, the construction of the recombinant vector pcDNATM3.1 / myc-His (-) A-TRIM21-myc and the construction of the OTUD4 siRNA are constructed; overexpression of TRIM21 or knockout of OTUD4 can significantly inhibit expression of SHBs / HBsAg protein in liver cancer cells, expression of HBsAg and HBeAg protein secreted out of the cells and the HBV DNA level.

The invention provides a BoNIA2b gene, which has a nucleotide sequence as shown in SEQ ID No.1, or a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function, wherein the nucleotide sequence is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1; the invention also provides an application thereof. Compared with the prior art, the broccoli BoNIA2b gene has the following advantages that functional verification is carried out on the broccoli BoNIA2b gene, it is found that overexpression of the BoNIA2b gene can promote increase of the content of glucoraphanin and sulforaphane in the hairy roots of broccoli, and it is found through further research that the content of glucoraphanin and sulforaphane in the hairy roots of broccoli can be increased; the overexpressed BoNIA2b gene is mainly used for regulating and controlling the expression level of some synthetic genes of glucoraphanin and sulforaphanin of the hairy roots of broccoli so as to regulate and control the synthesis of glucoraphanin and sulforaphanin. Finally, the BoNIA2b gene is proved to positively regulate the synthesis reaction of glucoraphanin and sulforaphane of broccoli.

The invention belongs to the field of tobacco gene engineering, and particularly relates to application of a ferulic acid synthetic gene NtKAT2.2 in tobacco. The nucleotide sequence of the tobacco ferulic acid synthetic gene NtKAT2.2 is as shown in SEQ ID No. 1 (sequence identifier number 1). The expression quantity of the NtKAT2.2 gene is in negative correlation with the content of ferulic acid in tobacco. Preliminary research results show that the NtKAT2.2 gene is highly related to the content of the ferulic acid in tobacco, and after the gene is silenced, the content of the ferulic acid in a tobacco strain after gene silencing can be obviously increased. Based on the result, the inventor believes that on the basis of further deep research on the gene, a good technical foundation is expected to be laid for tobacco quality improvement and further new variety cultivation.

Disclosed in the present invention is a use of genes NAT1 and bHLH110 in improving high-temperature resistance of rice, for use in using gene editing technology to obtain a rice material having enhanced high-temperature resistance. The base sequence of a rice gene NAT1 is as shown in SEQ ID NO. 1, and the base sequence of a gene bHLH110 is as shown in SEQ ID NO. 4. A base of a rice gene NAT1 of rice is replaced, deleted or inserted through gene editing technology to release inhibition on the rice gene bHLH110, and the expression of wax synthesis genes CER1 and CER1L is promoted by the rice gene bHLH110 to enhance the high-temperature resistance of a plant, thereby preparing a rice material having enhanced high-temperature resistance. According to the present invention, a NAT1 mutant rice material Nipponbare obtained by using CRISPR-Cas9 gene editing technology has remarkably enhanced high-temperature resistance, including an improved survival rate in a seedling stage and improved agronomic traits in a reproductive stage.

The invention discloses a genetic engineering strain with high yield of ergothioneine, a construction method of the genetic engineering strain and a method for producing ergothioneine, and belongs to the technical field of ergothioneine synthesis. The invention solves the problems of low ergothioneine yield and high production cost in the prior art. According to the genetic engineering strain with high yield of ergothioneine, streptomyces fradiae is used as an original strain, and ergothioneine synthesis genes POegt1 and POegt2 are simultaneously over-expressed in the original strain. The gene engineering strain is cultured to produce high-yield ergothioneine, and the production cost is saved. The genetic engineering strain provided by the invention is suitable for production of ergothioneine.

The invention relates to a method for producing irisin. The method comprises the following steps: providing an expression vector containing a nucleotide sequence for coding the irisin; inserting the expression vector into at least one first bacterium belonging to the Agrobacterium genus, thereby obtaining a first Agrobacterium comprising the expression vector; treating at least one plant material with the first agrobacterium comprising the expression vector, thereby obtaining a treated plant material; incubating the treated plant material so that it expresses the irisin; and extracting the irisin. The invention also relates to irisin contained in liposomes, their use as a medicament and route of administration, as well as a synthetic gene comprising a sequence encoding irisin and additional elements.

The present invention provides a recombinant strain for producing a 5-carbon compound or a polymer thereof and a use thereof. A strain which originally cannot produce a 5-carbon compound is modified, and a 5-carbon compound synthetic gene is added, so as to construct a recombinant strain capable of producing a 5-carbon compound or a polymer containing a 5-carbon compound monomer, so that production of high-added-value compounds such as 5-aminovaleric acid, 5-hydroxyvaleric acid and 5-hydroxyvaleric acid-related PHA is achieved.

A class of proteins that inhibit thrombin, particularly direct inhibitor of thrombin modified from sculptin, identified in the transcriptomics analysis of the salivary glands of ticks, as well as fragments and recombinant protein thereof, which can be used as anticoagulant agents and for the prophylaxis and / or treatment of thromboembolic diseases. These proteins fall within the fields of biochemistry, molecular biology, genetics, pharmacy and medicinal chemistry, being related to biochemical and metabolic processes.

The invention belongs to the technical field of synthetic biology genome editing, and relates to a chassis strain for gene cluster in-vivo traceless editing, a construction method and a gene cluster traceless editing method.The chassis strain is based on escherichia coli BL21 (DE3) and integrates a CRISPR-Cas9 system and a homologous recombination system (lambda Red) of a lambda bacteriophage source; and then knocking in an inducible sgRNA expression cassette (sgT) of a targeted editing vector to construct the vector. The strain has an efficient editing capability aiming at exogenous DNA, traceless editing of a target biosynthetic gene cluster is realized, and editing plasmids can be quickly eliminated, so that iterative editing is facilitated. The method is simple, convenient and rapid to operate and high in practicability, and provides powerful technical support for metabolic engineering and synthetic biology breeding transformation of streptomyces industrial strains, activation of synthetic gene clusters of recessive natural products and excavation of novel natural products.

The invention relates to the technical field of agricultural microorganisms, in particular to hopane synthetic gene combination and application thereof in enhancing environmental stress resistance of broad-host rhizobium, and particularly relates to 1) regulation and control of sucrose osmotic pressure stress tolerance of rhizobium; (2) regulating and controlling the salt stress tolerance of the rhizobium; 3) regulating and controlling the adaptive capacity of the rhizobium to high temperature; and (4) regulating and controlling the alkaline environment adaptability of the rhizobium.

Methods for making synthetic gene clusters are described.

A synthetic expression regulator for a plant gene is disclosed, related to a phenotypic trait of male sterility, and a process for producing male sterile plants using synthetic regulators and use thereof for breeding hybrid crop varieties.

The invention belongs to the technical field of biology, and relates to a synthesized gene tandem expression cassette and application thereof. According to the invention, the genes cry1Ab, vip3Aa, gr79-epsps and gat are combined into a gene tandem expression cassette form and are integrated into a corn material, so that compared with a single-gene insect-resistant or herbicide-resistant gene adopted in the prior art, the resistance of transgenic corn to lepidoptera pests is remarkably improved, the tolerance to glyphosate also reaches a high resistance level, and the application prospect is wide. Especially, the insect-resistant genes have a synergistic effect, and the effect of quickly killing insects is achieved.

The invention belongs to the technical field of genetic engineering, and discloses an engineering bacterium capable of producing Ectoine at high yield as well as a preparation method and application of the engineering bacterium. Escherichia coli W3110 is used as a chassis cell, and an Ectoin metabolic pathway is constructed by using synthetic biology. A constitutive promoter Pglgs of a transcription factor related gene in escherichia coli is preferably selected, an ectBAC for coding an Ectoine synthetic gene is preferably selected as a reporter gene, a constitutive plasmid for expressing the Ectoine synthetic gene is constructed, the expression level of the gene is improved, an engineering bacterium Escherichia coli ECT-W020 with high yield of Ectoine is obtained, and the preservation number of the engineering bacterium Escherichia coli ECT-W020 is GDMCC No: 65012. When the engineering bacterium is used for producing Ectoin, the process of adding an inducer is omitted, and the purpose of reducing the production cost is achieved.

Aspects of the invention is directed to a high-throughput method for screening a gene circuit library. The pace of gene circuit engineering can thereby be increased by expanding the number of circuits tested in each cycle. By measuring large collections of circuits in a single experiment, such an approach could be used for rapidly profiling complex circuit design spaces to identify part compositions with desired quantitative behaviors.

The present invention discloses an agmatine coumaroyl transferase gene HvACT2 and its application in improving the disease resistance of barley. The present invention clones and analyzes the barley agmatine coumaroyl transferase synthesis gene HvACT2 for the first time, confirms the function of the HvACT2 gene in the synthesis of hydroxycinnamic acid amide and malt guanidine by transgenic means for the first time, and finds that overexpression of HvACT2 can significantly improve the disease resistance of barley. The present invention also finds that HvACT2 has the function of catalyzing the synthesis of p-coumaroyl agmatine and feruloyl agmatine. The present invention also finds that the transient expression of HvACT2 synthesizes a variety of agmatine amides in tobacco, and tobacco does not originally have such substances, that is, the expression of exogenous genes causes tobacco to synthesize agmatine amides, and it is also found that the expression of HvACT2 in tobacco also increases the content of caffeoyl putrescine.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a DpCYP707A2 gene for regulating and controlling synthesis of dioscin and application of the DpCYP707A2 gene. The nucleotide sequence of the DpCYP707A2 gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The result of the embodiment of the invention shows that the Chinese yam DpCYP707A2 gene provided by the invention can regulate and control the synthesis of dioscin, and the DpCYP707A2 gene plays an important role in influencing the synthesis of the dioscin of plants, can help to breed new Chinese yam varieties with increased dioscin content, and has wide application value.

To provide artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types.SOLUTION: An artificial expression construct comprises: (i) a concatemer of a specific sequence; (ii) a promoter; and (iii) a heterologous encoding sequence. The heterologous encoding sequence encodes an ion transporter, enzyme, transcription factor, receptor, membrane protein, cellular trafficking protein, signaling molecule, neurotransmitter, calcium reporter, channel rhodopsin, or CRISPR / CAS molecule etc., associated with a capsid that crosses the blood brain barrier. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2 / 3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections.SELECTED DRAWING: None

The invention provides an in-vitro transcription method based on RNA polymerase and a kit, which are suitable for the fields of mRNA synthesis, gene research and biological pharmacy. According to the method, the problems of low efficiency, poor stability and the like of traditional in-vitro transcription are solved through an optimization technology. The method comprises the following specific steps: providing a linear DNA template containing a T7 promoter; according to the method, site-directed mutagenesis or fusion protein modified high-activity T7RNA polymerase is combined with chemically modified NTPs and a spermine-BSA compound stabilizer to react under the conditions that the temperature is 37-42 DEG C and the pH is 7.5-8.5, and formation of double-stranded RNA is promoted through dynamic temperature control. A reaction system contains Mg < 2 + > / Mn < 2 + > and DTT, and enzyme activity and product integrity are optimized. The kit comprises an improved reagent and a standardized process, the transcription efficiency is improved by 2-5 times, the product yield is greater than or equal to 80%, and the activity is not lost after the kit is stored at 4 DEG C for 1 month. The method has the technical advantages that NTPs degradation resistance is achieved, template preparation is simplified, universality is high, the method is suitable for large-scale production of mRNA vaccines, gene therapy and the like, the production cost is remarkably reduced, and wide application of the in-vitro transcription technology is promoted.

The present invention provides a method for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, the method comprising modifying the tobacco plant or the tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides the use of a diterpene synthesis gene for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, and tobacco cells, tobacco plants, tobacco plant propagation materials, leaves harvested, processed tobacco or tobacco products obtainable according to the present invention.

The present invention discloses a method for activating the expression of a silent secondary metabolite synthesis gene cluster (BGC) of actinomycetes, and relates to the technical field of microbial secondary metabolites. The technical key points are as follows: the method uses microbial quorum sensing signal molecules to stimulate the silent gene cluster of actinomycetes, so that the silent BGCs of actinomycetes are activated; the quorum sensing signal molecules used include but are not limited to Farnesol, Tyrosol, 3‑oxo‑C12‑HSL and Tryptophol. The method of the present invention is a novel method for activating the expression of silent gene clusters of actinomycetes, that is, a strategy for rapidly and efficiently activating silent BGCs in different actinomycetes. The method of the present invention will provide an effective way to activate the treasure house of silent BGCs existing in large quantities in nature and to deeply explore new actinomycete natural products, thereby contributing more and better new lead compounds to the research and development of multiple fields such as medicine and agriculture.

Methods for identifying biosynthetic gene clusters that include genes for producing compounds that interact with specific target proteins are disclosed. Some methods relate to bioinformatics methods for identifying and / or prioritizing biosynthetic gene clusters. Related systems, components, and tools for the identification and expression of such gene clusters are also disclosed.

This invention belongs to the field of tobacco genetic engineering, specifically relating to the application of the ferulic acid synthesis gene NtKAT2.2 in tobacco. The tobacco ferulic acid synthesis gene... NtKAT2.2 The nucleotide sequence is shown in SEQ ID No. 1. NtKAT2.2 Gene expression levels are negatively correlated with ferulic acid content in tobacco. Preliminary research results indicate that the gene involved in this application... NtKAT2.2 The gene is highly correlated with ferulic acid content in tobacco. Silencing this gene significantly increases ferulic acid content in the resulting tobacco lines. Based on this result, the inventors believe that further in-depth research on this gene could lay a solid technical foundation for improving tobacco quality and developing new varieties.