117 results about "Synthetic gene" patented technology

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention provides a BoNIA2b gene, which has a nucleotide sequence as shown in SEQ ID No.1, or a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function, wherein the nucleotide sequence is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1; the invention also provides an application thereof. Compared with the prior art, the broccoli BoNIA2b gene has the following advantages that functional verification is carried out on the broccoli BoNIA2b gene, it is found that overexpression of the BoNIA2b gene can promote increase of the content of glucoraphanin and sulforaphane in the hairy roots of broccoli, and it is found through further research that the content of glucoraphanin and sulforaphane in the hairy roots of broccoli can be increased; the overexpressed BoNIA2b gene is mainly used for regulating and controlling the expression level of some synthetic genes of glucoraphanin and sulforaphanin of the hairy roots of broccoli so as to regulate and control the synthesis of glucoraphanin and sulforaphanin. Finally, the BoNIA2b gene is proved to positively regulate the synthesis reaction of glucoraphanin and sulforaphane of broccoli.

Disclosed in the present invention is a use of genes NAT1 and bHLH110 in improving high-temperature resistance of rice, for use in using gene editing technology to obtain a rice material having enhanced high-temperature resistance. The base sequence of a rice gene NAT1 is as shown in SEQ ID NO. 1, and the base sequence of a gene bHLH110 is as shown in SEQ ID NO. 4. A base of a rice gene NAT1 of rice is replaced, deleted or inserted through gene editing technology to release inhibition on the rice gene bHLH110, and the expression of wax synthesis genes CER1 and CER1L is promoted by the rice gene bHLH110 to enhance the high-temperature resistance of a plant, thereby preparing a rice material having enhanced high-temperature resistance. According to the present invention, a NAT1 mutant rice material Nipponbare obtained by using CRISPR-Cas9 gene editing technology has remarkably enhanced high-temperature resistance, including an improved survival rate in a seedling stage and improved agronomic traits in a reproductive stage.

The invention discloses a genetic engineering strain with high yield of ergothioneine, a construction method of the genetic engineering strain and a method for producing ergothioneine, and belongs to the technical field of ergothioneine synthesis. The invention solves the problems of low ergothioneine yield and high production cost in the prior art. According to the genetic engineering strain with high yield of ergothioneine, streptomyces fradiae is used as an original strain, and ergothioneine synthesis genes POegt1 and POegt2 are simultaneously over-expressed in the original strain. The gene engineering strain is cultured to produce high-yield ergothioneine, and the production cost is saved. The genetic engineering strain provided by the invention is suitable for production of ergothioneine.

The present invention provides a recombinant strain for producing a 5-carbon compound or a polymer thereof and a use thereof. A strain which originally cannot produce a 5-carbon compound is modified, and a 5-carbon compound synthetic gene is added, so as to construct a recombinant strain capable of producing a 5-carbon compound or a polymer containing a 5-carbon compound monomer, so that production of high-added-value compounds such as 5-aminovaleric acid, 5-hydroxyvaleric acid and 5-hydroxyvaleric acid-related PHA is achieved.

The invention belongs to the technical field of synthetic biology genome editing, and relates to a chassis strain for gene cluster in-vivo traceless editing, a construction method and a gene cluster traceless editing method.The chassis strain is based on escherichia coli BL21 (DE3) and integrates a CRISPR-Cas9 system and a homologous recombination system (lambda Red) of a lambda bacteriophage source; and then knocking in an inducible sgRNA expression cassette (sgT) of a targeted editing vector to construct the vector. The strain has an efficient editing capability aiming at exogenous DNA, traceless editing of a target biosynthetic gene cluster is realized, and editing plasmids can be quickly eliminated, so that iterative editing is facilitated. The method is simple, convenient and rapid to operate and high in practicability, and provides powerful technical support for metabolic engineering and synthetic biology breeding transformation of streptomyces industrial strains, activation of synthetic gene clusters of recessive natural products and excavation of novel natural products.

Methods for making synthetic gene clusters are described.

Aspects of the invention is directed to a high-throughput method for screening a gene circuit library. The pace of gene circuit engineering can thereby be increased by expanding the number of circuits tested in each cycle. By measuring large collections of circuits in a single experiment, such an approach could be used for rapidly profiling complex circuit design spaces to identify part compositions with desired quantitative behaviors.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a DpCYP707A2 gene for regulating and controlling synthesis of dioscin and application of the DpCYP707A2 gene. The nucleotide sequence of the DpCYP707A2 gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The result of the embodiment of the invention shows that the Chinese yam DpCYP707A2 gene provided by the invention can regulate and control the synthesis of dioscin, and the DpCYP707A2 gene plays an important role in influencing the synthesis of the dioscin of plants, can help to breed new Chinese yam varieties with increased dioscin content, and has wide application value.

The invention provides an in-vitro transcription method based on RNA polymerase and a kit, which are suitable for the fields of mRNA synthesis, gene research and biological pharmacy. According to the method, the problems of low efficiency, poor stability and the like of traditional in-vitro transcription are solved through an optimization technology. The method comprises the following specific steps: providing a linear DNA template containing a T7 promoter; according to the method, site-directed mutagenesis or fusion protein modified high-activity T7RNA polymerase is combined with chemically modified NTPs and a spermine-BSA compound stabilizer to react under the conditions that the temperature is 37-42 DEG C and the pH is 7.5-8.5, and formation of double-stranded RNA is promoted through dynamic temperature control. A reaction system contains Mg < 2 + > / Mn < 2 + > and DTT, and enzyme activity and product integrity are optimized. The kit comprises an improved reagent and a standardized process, the transcription efficiency is improved by 2-5 times, the product yield is greater than or equal to 80%, and the activity is not lost after the kit is stored at 4 DEG C for 1 month. The method has the technical advantages that NTPs degradation resistance is achieved, template preparation is simplified, universality is high, the method is suitable for large-scale production of mRNA vaccines, gene therapy and the like, the production cost is remarkably reduced, and wide application of the in-vitro transcription technology is promoted.

The present invention provides a method for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, the method comprising modifying the tobacco plant or the tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides the use of a diterpene synthesis gene for increasing the sucrose ester content in a tobacco plant or a tobacco cell culture, and tobacco cells, tobacco plants, tobacco plant propagation materials, leaves harvested, processed tobacco or tobacco products obtainable according to the present invention.

Methods for identifying biosynthetic gene clusters that include genes for producing compounds that interact with specific target proteins are disclosed. Some methods relate to bioinformatics methods for identifying and / or prioritizing biosynthetic gene clusters. Related systems, components, and tools for the identification and expression of such gene clusters are also disclosed.

This invention belongs to the field of tobacco genetic engineering, specifically relating to the application of the ferulic acid synthesis gene NtKAT2.2 in tobacco. The tobacco ferulic acid synthesis gene... NtKAT2.2 The nucleotide sequence is shown in SEQ ID No. 1. NtKAT2.2 Gene expression levels are negatively correlated with ferulic acid content in tobacco. Preliminary research results indicate that the gene involved in this application... NtKAT2.2 The gene is highly correlated with ferulic acid content in tobacco. Silencing this gene significantly increases ferulic acid content in the resulting tobacco lines. Based on this result, the inventors believe that further in-depth research on this gene could lay a solid technical foundation for improving tobacco quality and developing new varieties.

The invention discloses a honeysuckle flower pigment synthesis gene and application thereof in regulating and controlling the content and resistance of plant anthocyanidin, and belongs to the technical field of gene engineering. The nucleotide sequence of the honeysuckle flower pigment synthesis gene is as shown in SEQ ID NO. 6. The invention provides a honeysuckle flower pigment synthesis gene. The gene has the effects of regulating and controlling the content of anthocyanidin in plants and regulating and controlling the stress resistance of the plants. The gene is introduced into a plant body by constructing an overexpression vector, so that the gene is stably overexpressed, the anthocyanidin content in the plant is remarkably increased, and the petal color and seed coat color of the plant are darkened; the resistance of the plant to drought stress and salt stress is also remarkably enhanced, so that the plant can adapt to a severe environment more easily, and a new gene resource and a theoretical support are provided for breeding of high-resistance plant varieties.

Methods, systems and apparatus are described for generating synthetic gene expression profile datasets for downstream biological / pharmacological analysis, processing and / or applications Generating synthetic gene expression profile datasets includes: receiving a gene expression profile dataset comprising data representative of at least one gene expression profile; computing a complex gene expression profile dataset based on applying a discrete Fourier transform to data representative of each gene expression profile of the received gene expression profile dataset; sampling the complex gene expression profile dataset based on using a statistical distribution to sample and modify a predetermined number of one or more components of each complex gene expression profile for generating a plurality of synthetic complex gene expression profiles; computing a real-valued synthetic gene expression profile dataset based on applying an inverse discrete Fourier transform to the synthetic complex gene expression profile dataset; and outputting data representative of the real-valued synthetic gene expression profile dataset for downstream biological / pharmacological analysis, processing and / or applications.

The invention belongs to the technical field of biology, and particularly relates to a genetically engineered bacterium for producing a heparin precursor and a construction method and application of the genetically engineered bacterium. According to the technical scheme adopted by the invention, a lipopolysaccharide (LPS) synthetic gene waaJ and a claic acid synthetic gene cluster wca are knocked out, and the synthesis of LPS and CA is blocked, so that more carbon sources enter a heparin precursor synthetic route, and the yield and / or conversion rate of the heparin precursor are / is improved. According to the invention, heparin precursor synthetases KfiA (UDP-GlcNAc glycosyl transferase), KfiC (UDP-GlcA glycosyl transferase) and KfiD (UDP-glucose dehydrogenase) are further enhanced, the expression of UDP-GlcA generated by UDP-Glc is catalyzed, and the yield and / or conversion rate of the heparin precursor can be further improved.

The invention discloses a sequence optimization method for improving the stability of circular RNA, application and a product, and belongs to the technical field of biotechnology and molecular biology. In order to solve the problem that the existing circular RNA is easy to be specifically degraded by RNAseK and lysosome after entering a cell, synonymous mutation optimization is carried out on an open reading frame of a target gene of the circular RNA by identifying nucleic acid motifs (a first type of nucleic acid motifs and a second type of nucleic acid motifs) identified by two degradation mechanisms. The optimized circular RNA is prepared by means of in-vitro cyclization of an anabaena I-type intron self-splicing mediator, after cells are transfected, the intracellular RNA expression level of the circular RNA is remarkably improved compared with that of a wild type, and the target protein expression efficiency is also remarkably improved. The intracellular stability and expression efficiency of the circular RNA are remarkably enhanced, and the method can be widely applied to the scenes of gene expression regulation, protein synthesis, gene therapy, vaccine development and the like.

The present application relates to a kind of cyclic ester peptide compound aglomycin A biosynthesis gene cluster and its application.The nucleotide sequence of the gene cluster is as shown in SEQ ID NO.1, and the use is to produce peptide compound aglomycin A or peptide compound aglomycin D.

Various devices, systems, structures and methods are disclosed related to securely authorizing a transaction by synchronizing digital genomic data with associated synthetic genomic variants. An embodiment of the present invention utilizes digital genomic data associated with an entity, such as a person, who may utilize a genome-based security device to complete a transaction. In one embodiment, a person may use a genome-based security device to communicate with an external device over a wireless or other communication interface, synchronize digital genomic data and an associated synthetic variant received from the external device with digital genomic data and associated synthetic variant stored on the genome-based security device.

The present invention belongs to the field of biochips, and in particular relates to a substrate for in-situ synthesized gene chip probes manufactured by a combination method. Specifically, a single starting base is densely planted on the substrate and surface passivation is completed. Then, according to the actual needs of the starting bases for future in-situ synthesized gene chip probes, a combination is intercepted from the substrate with a single starting base densely planted, and a substrate for in-situ synthesized gene chip probes is made on a support carrier by a combination method. This invention has more advantages when used in conjunction with the technology of in-situ synthesizing gene chip probes using DNA polymerase. This invention can avoid the difficulty in site selection during substrate activation, perform the same treatment on all activation sites, and reduce the manufacturing cost of the substrate for gene chip probe synthesis.

The invention belongs to the technical field of lipopeptide preparation, genetic engineering and biological medicine, and particularly relates to novel double-fat-chain lipopeptide as well as a preparation method and application thereof. According to the invention, a novel artificial gene cluster rhc is successfully constructed through precise editing and recombination of a functional module of a natural non-ribosomal lipopeptide gene cluster, and after heterologous expression of the gene cluster, a brand new double-fat-chain lipopeptide product Rhcolide A can be synthesized; the rhc gene cluster used for preparing the novel double-fat-chain lipopeptide Rhcolide A is the first artificial double-fat-chain lipopeptide synthetic gene cluster constructed through genetic engineering modification. A biological function test verifies and finds that Rhcolide A has excellent ferroptosis sensitizing activity, can be used as a ferroptosis sensitizer to be combined with a ferroptosis inducer to prepare a non-small cell carcinoma resisting drug, provides a brand new drug molecular entity for treatment of non-small cell carcinoma, and has important clinical application value and industrialization prospect.

The application discloses a method for biosynthesis of gene or gene cluster product based on chromatin three-dimensional structure, and belongs to the field of genetic engineering. The method can realize the improvement of the target gene or gene cluster product by the directional integration of the target gene or gene cluster into the strong correlation chromatin region with high frequency interaction on the genome. The method has the characteristics of simple implementation, good repeatability, remarkable effect and the like, and can be widely applied to the improvement of the target gene or gene cluster product.

Disclosed herein are shelf-stable compositions based on synthetic gene networks and / or cell-free systems that are lyophilized on a solid support. The compositions can be easily transported and stored for a period of time, and activation can be done by simply adding water. Methods of use are also disclosed herein, including, but are not limited to, sensing and a variety of logic functions. The invention permits straightforward, sterile and abiotic distribution of synthetic biology-based technology to clinical settings, food processing and industry, the military and consumer products.

The invention discloses a bacillus velezensis ATC-AL and application thereof. The preservation number of the strain is CCTCC (China Center For Type Culture Collection): M20251280. A genome of the bacillus velezensis ATC-AL has a synthetic gene cluster for synthesizing various antifungal active substances, the active substances of the bacillus velezensis ATC-AL can destroy cell surface structures of pathogenic bacteria, induce cell apoptosis and inhibit spore development of pathogenic fungi, and the bacillus velezensis ATC-AL has a broad-spectrum inhibition effect on various pathogenic fungi such as sweet potato coracocystis canaliculata, fusarium graminearum and aspergillus flavus; the generation inhibition rate of vomitoxin reaches 95%, and the generation inhibition rate of aflatoxin B1 can reach 100%; fungus infection experiments on sweet potatoes, corn and wheat prove that active substances in a strain cell fermentation broth can completely inhibit the decay effect of sweet potato coracocystis longirostris, fusarium graminearum and aspergillus flavus on the food crops; according to the invention, a microbial control resource library for biological control of pathogenic fungi and toxin pollution thereof is enriched.

The present invention relates to a synthetic gene cluster of an enfumafungin antibiotic and a synthetic method therefor. Specifically, in the present invention, key functional genes in the linking of a β-D-glucopyranose at position C3 of a fernane-type framework, the oxidation at position C2 into α-OH, the oxidative cleavage of ring E at C19-C20, and the acetylation of hydroxyl at position C2 during the biosynthesis of an enfumafungin antibiotic, i.e. fuscoatroside, are isolated, wherein the genes are named fsoA, fsoD, fsoE and fsoF, respectively. The present invention also provides encoding polypeptides thereof. Provided in the present invention are an artificial fusion enzyme gene, i.e. efuA(TC)fsoA(GT); and on the basis of the artificial fusion enzyme gene, the heterologous expression of four genes, i.e. efuA(TC)fsoA(GT), fsoD, fsoE and fsoF, can synthesize an enfumafungin precursor (13). The present invention clarifies an FsoE-mediated C-C bond breaking function of a P450 enzyme, and provides a key catalytically active residue of FsoE. The present invention reports the biosynthetic pathway of such compound for the first time, and establishes an important foundation for the green and efficient synthesis of the compound.

The invention discloses a gene detection management system and method. The method comprises the following steps: S1, generating a standardized high-dimensional gene detection data matrix for training; s2, performing global feature analysis on the high-dimensional gene detection data matrix; s3, designing a forward diffusion process and a reverse generation process of the Diffusion model based on a result of the global feature analysis; s4, carrying out global pre-training on the Diffusion model; s5, performing regional layering processing on the Diffusion model based on the selected specific region; s6, carrying out local fine tuning training on the layered Diffusion model; s7, introducing bioinformatics priori knowledge as a constraint condition to regulate and control a reverse generation process of the Diffusion model; and S8, generating synthetic gene detection data by using the Diffusion model subjected to local fine tuning training. The gene mutation mode matching degree is improved by 13.5%, and the expression consistency of key gene loci is improved by 9.8%.

The invention belongs to the technical field of plant biology, and particularly relates to a nucleic acid aptamer with high binding force specificity with arabidopsis thaliana COP1 protein and a preparation method of the nucleic acid aptamer. In order to solve the problem that the existing screening technology wastes time and labor, the invention provides a nucleic acid aptamer specifically bound with arabidopsis thaliana COP1 protein and a preparation method thereof. The nucleic acid aptamer Lib2-11 of arabidopsis thaliana COP1 is obtained through a One-step screening technology, the nucleic acid aptamer has high binding property and high specificity, and the nucleic acid aptamer Lib2-11c with high binding property is obtained after a sequence is optimized. The nucleic acid aptamers Lib2-11 and Lib2-11c disclosed by the invention have important application prospects in determining the protein level of the arabidopsis thaliana COP1, subcellular localization and a light-operated synthesis gene route.

The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and / or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and / or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.