43 results about "Arabinogalactan" patented technology

The invention discloses an efficient sparassis crispa bottle cultivation method, and belongs to the technical field of edible fungus cultivation. The bottle cultivation method comprises the steps of cultivation raw material preparation, compost preparation, fruiting bottle production, inoculation, fungus bottle culture, primordium induction and sporocarp culture. Specific fermentation raw materials are adopted to replace or partially replace pine sawdust to serve as cultivation main materials, humic acid, citrulline and arabinogalactan serve as additives, and efficient production of sparassis crispa is achieved in a bottle cultivation mode. According to the cultivation method, the primordium is easy to form, the consistency is good, the operation is simple, the biological conversion rate can reach 65-70%, and the growth and development period is 75-90 days.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The invention relates to a method for simultaneously preparing arabinogalactan and dihydroquercetin on the basis of larch, which comprises the following steps: selecting root wood after rotation of larch, clearly crushing the root wood to obtain the used raw material small sawdust, performing enzymolysis and pressurized water extraction, and simultaneously combining membrane separation and membrane concentration technologies to obtain the arabinogalactan and dihydroquercetin. Meanwhile, an arabinogalactan product and 99% of dihydroquercetin product are prepared. Extraction adopts enzymolysis, pressurization and low-temperature water extraction, the tight structure of the raw material is destroyed through cellulase and hemicellulase, the seepage force and penetrating force of water are increased through pressurization, the extraction efficiency is improved, then existing dihydroquercetin glycosides are subjected to enzymolysis through beta-glucosidase to form free dihydroquercetin, the yield is improved, and the method is suitable for industrial production. The method is simple in operation, combines membrane separation and membrane concentration technologies, avoids the defects of traditional normal-pressure water extraction, and compared with traditional organic reagent extraction and ether ester extraction, the method does not use organic reagents in the whole process, avoids environmental pollution, and is safe, feasible and low in cost.

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

The antioxidant activity of alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) and of alpha-tocopherol succinate (TS) has been examined in isolated hepatocytes and microsomal fractions from rat liver. Both TPGS and TS require esterase activity to yield free alpha-tocopherol and, hence, antioxidant activity. TPGS and TS consistently exerted a more effective antioxidant protection than an equivalent amount of directly-added free alpha-tocopherol. The low antioxidant efficiency of directly added free alpha-tocopherol in such water-based experimental systems as used here seems to be due to its extreme hydrophobicity. TPGS, on the other hand, is an extremely hydrophilic compound which is being examined as a useful source of alpha-tocopherol in certain clinical situations and is here shown to be a convenient and effective source for experimental studies into lipid peroxidation and antioxidant mechanisms.

The application discloses application of an extract of Epiphyllum crenatum in preparation of a medicine for treating and improving intestinal diseases, relates to the fields of phytochemistry and microbiological technology, and the extract of the Epiphyllum crenatum is type I arabinogalactan. 6 The average molecular weight of the extract of the Epiphyllum crenatum extracted from petals of the Epiphyllum crenatum is 5.767*10 6 Da, and the backbone structure is a polysaccharide of β-Galp connected through (1→4) bonds, which expands the application potential of the Epiphyllum crenatum, improves the value of the Epiphyllum crenatum, and provides a new raw material for the field of the medicine for treating and improving intestinal diseases.

The invention relates to a method for extracting dihydroquercetin and arabinogalactan from larch, which is characterized by comprising the following steps: by taking larch root as a raw material, carrying out ultrasonic extraction once by using a natural surfactant assisted ethanol solution, adding ethanol into the remaining extracting solution, precipitating crude polysaccharide in the extracting solution in an alcohol precipitation manner, filtering after alcohol precipitation, and drying to obtain the dihydroquercetin and arabinogalactan. Drying the filter cake in a drying oven to obtain crude polysaccharide (containing arabinogalactan); adding an acid and an organic solvent into the filtrate, completely converting glycoside into aglycone in an acid hydrolysis manner, separating out an organic solvent phase after the reaction is finished, concentrating and directly drying the organic solvent, dissolving a solid matter with hot water, enriching with macroporous adsorption resin, eluting, and recrystallizing to obtain a product. The operation process has the main characteristics that the raw materials can be directly used without drying, the energy consumption is low, the macroporous adsorption resin purification process is pollution-free, the production efficiency is high, and the yield is high; all solvents can be recycled, so that industrial large-scale production is facilitated.

This invention discloses a composition for enhancing immunity through gut microbiota, its preparation method, and its application. The composition comprises the following raw materials: a complex prebiotic, polyphenols, inactivated *Saccharomyces boulardii*, and plant extracts. The complex prebiotic includes human milk oligosaccharides and arabinogalactan, the polyphenols include berry polyphenols and grape polyphenols, and the plant extracts include pomegranate seed extract and kale extract. This invention's composition, on the one hand, selectively enriches *A. AKK* bacteria through prebiotics; *A. AKK* can directly interact with various immune cells to enhance immunity. On the other hand, supplementing with yeast factors can also inhibit inflammation and enhance immune responses, thereby significantly increasing the number of *A. AKK* bacteria in the gut microbiota and simultaneously improving intestinal cellular immune function.

The invention discloses araban obtained from fructus lycii and application of the araban. According to the molecular structure of the araban, a main chain is formed by repeated-> 5)-alpha-Araf-(1-> structural units, and the C-2 position of each sugar residue is substituted by T-alpha-Araf-(1-> structural units. According to the method, free protein is removed through a water extraction and alcohol precipitation method and an alkali solution method, graded alcohol precipitation and purification are combined, and the araban component only containing arabinose is extracted and separated from the fructus lycii for the first time. Compared with an arabinogalactan component, the arabinogalactan component has the advantages that the phagocytic ability of macrophages is remarkably improved, the macrophages are induced to secrete NO, the activity of acid phosphatase is improved, the phagocytic ability of the macrophages and the activity of the acid phosphatase can be activated in an LPS-induced inflammation model, NO secretion is inhibited, and the arabinogalactan component can be used for preparing an LPS-induced inflammation model. Therefore, an anti-inflammatory effect is generated, and a better bidirectional immunoregulation effect is achieved.

This invention discloses a method for preparing magnetic nanobeads for cell sorting. Using hemicellulose, arabinogalactan, FeCl3, FeCl2, and Na2CO3 as raw materials and DMSO as solvent, monodisperse hemicellulose / arabinogalactan@Fe3O4 magnetic nanobeads are generated by a solvothermal reaction. Subsequently, the nanobeads are further activated by 4-fluorobenzenesulfonyl chloride to introduce fluorobenzenesulfonyl groups. The fluorobenzenesulfonyl groups are then coupled with cell sorting antibody CD3. The coupled antibody magnetic beads are then used to purify and sort cells.

The invention provides a method for improving the stability of dehalogenase through an arabinogalactan covalent cross-linking immobilization technology. The method comprises the steps of constructing and expressing a recombinant dehalogenase strain, purifying the recombinant dehalogenase and carrying out dehalogenase covalent cross-linking immobilization. The arabinogalactan is used for covalently crosslinking the dehalogenase, so that the thermal stability of the dehalogenase and the stability of the dehalogenase under different pH conditions and in an organic solvent can be remarkably improved.

The present invention provides uses of enzymes for cleaving a linkage between two D-galactofuranoses and / or two D-arabinofuranoses in a polysaccharide. An example of a polysaccharide where such linkages are present is arabinogalactan. The invention also provides compositions comprising said enzymes, as well as methods for preparing a sample, and methods for determining the presence of a bacterium of the Actinomycetota phylum in a sample using said enzymes. Additionally, the invention provides pharmaceutical formulations comprising a composition of the invention, as well as uses of the pharmaceutical formulations as a medicament, for example to treat a bacterial infection caused by a bacterium of the Actinomycetota phylum.

The application discloses a litchi functional ingredient rich in polyphenols and arabinogalactan and a preparation method thereof, and belongs to the technical field of deep processing of agricultural products and functional food. The preparation method specifically comprises the following steps: (1) litchi pretreatment; (2) fruit pulp fermentation; and (3) drying and powdering. The application provides a novel preparation method different from a traditional "extraction-purification-application" route, and under the premise of maintaining the natural properties of raw materials, the structure optimization of polyphenols and the enrichment of arabinogalactan are simultaneously realized in a litchi raw material system by means of lactic acid bacteria fermentation and a mild and controllable processing mode, so that the litchi functional ingredient directly applicable is obtained.

The present invention relates to a palatable, free-flowing powdered composition derived from okra (Abelmoschus esculentus) for enhancing endurance. The composition comprises polysaccharides (5-50%), including Rhamnogalacturonan and Arabinogalactan; flavonoid glycosides (2-10%), including Quercetin 3-O-gentiobioside and Isoquercetin (Okraendurosides); and total carbohydrates (30-70%), with optional excipients for stability and bioavailability. The composition on administration to subjects in the appropriate dosage improves running endurance, enhances swimming endurance, improves rate of perceived Exertion (RPE), improves fatigue severity, improves fatigue score, increases peak power and enhances endurance and muscle performance. The invention also discloses a method of preparation involving hydroalcoholic extraction, concentration, and purification, resulting in a water-soluble powder. The composition can be formulated into capsules, tablets, powders, and beverages and is administered orally in doses of 100-2000 mg per day. Animal and human studies confirm its efficacy in enhancing endurance, making it applicable in nutraceutical, sports nutrition, and pharmaceutical industries.

The invention belongs to the technical field of molecular markers, and particularly relates to a KASP molecular marker related to wheat agronomic traits and application of the KASP molecular marker. The nucleotide sequence of the KASP molecular marker is shown as SEQ ID NO.2, and single nucleotide T-G mutation occurs at the 101bp site. The molecular marker is a KASP molecular marker developed by aiming at an SNP (Single Nucleotide Polymorphism) site of a TaFLA62 promoter region of a bundled arabinogalactoprotein-like gene. The mutation from T to G at the 101bp of the KASP molecular marker can reduce the wheat plant height and / or increase the grain weight. The molecular marker provided by the invention can efficiently detect and track a mutation site of a TaFLA62 gene promoter region in a wheat variety / strain, and provides technical support for wheat molecular breeding.

The invention belongs to the field of biological medicine, and particularly relates to an antiviral composition and application thereof. The invention discloses an antiviral effect of breast milk oligosaccharide and arabinogalactan and an antiviral mechanism of the breast milk oligosaccharide and arabinogalactan. The invention also discovers that the compound polysaccharide composed of 2 '-fucosyllactose and arabinogalactan at low concentration can play a role in inhibiting influenza virus, the 2'-fucosyllactose and arabinogalactan play a synergistic effect, and the synergistic effect is associated with the drug concentration and the proportional relationship of the 2 '-fucosyllactose and arabinogalactan. According to the related discovery, the compound polysaccharide can be used for preparing foods, dietary nutrition supplements and medicines to improve the antiviral ability and immunity of subjects.

The invention discloses an induction substrate, a preparation method thereof, an application of the induction substrate in galactanase production and an enzyme preparation, and belongs to the technical field of microbial fermentation. The induction substrate provided by the invention is a product obtained by carrying out selective hydrolysis and post-treatment on Arabic gum or arabinogalactan through an acid-ethanol solution. The preparation method of the induction substrate comprises the steps of preparing the acid-ethanol solution, feeding, carrying out selective hydrolysis, neutralizing, precipitating, washing, drying and the like. The invention also provides a method for efficiently fermenting and producing the galactanase by using the induction substrate at a specific opportunity (the glucose concentration of fermentation liquor is lower than 0.2 g / L) and combining two-stage temperature control, and an enzyme preparation obtained by the method. The induction substrate is high in specificity, the yield of the beta-1, 6-galactanase can be remarkably increased, the raw materials are cheap, the process is simple, and the induction substrate is very suitable for industrial production.

The invention belongs to the technical field of high polymer materials, and discloses a design method of rare earth-polysaccharide based multifunctional hydrogel. The hydrogel takes arabinogalactan as a matrix and specifically comprises the following components: arabinogalactan, acrylamide, polyethylene glycol and nano copper powder, and europium ions are doped in the formed polysaccharide-based hydrogel. The hydrogel has fluorescent and conductive dual sensing channels, overcomes the disadvantage that the traditional rare earth hydrogel can only be used as a fluorescent sensor, and provides a stronger network and enhances mechanical properties by taking rare earth-polysaccharide as physical crosslinking. Meanwhile, the hydrogel can freely adjust the mechanical property and the fluorescence intensity by changing the polysaccharide content, responds to the external ion environment, and has extremely high sensing sensitivity (GF = 21) and tensile property (strain = 1100%). The hydrogel sensor is stable in performance and convenient to prepare, the application range of the rare earth hydrogel is widened, and the hydrogel sensor has a good development prospect.

This invention relates to a method for improving the intestinal tolerance of wheat bran dietary fiber based on synergistic modification by microorganisms and enzymes. The method includes ultra-fine grinding and high-pressure moist heat activation of wheat bran; multi-enzyme synergistic microbial stepwise treatment, first using high-temperature amylase and neutral protease to remove interfering components such as starch and protein, then using laccase to degrade lignin, resulting in a looser structure; subsequently, phytase efficiently degrades phytic acid, Aspergillus nidulan degrades the side chains of arabinogalactan, and snail enzyme and ferulic acid esterase work synergistically to convert insoluble arabinoxylan into soluble dietary fiber and release phenolic antioxidants. This method increases the soluble dietary fiber content of wheat bran by 2 times, reduces phytic acid to 10 mg / g, increases free phenolic acid content by 40%, achieves a DPPH free radical scavenging rate of 60%, significantly enhances antioxidant activity, and significantly reduces IgG levels in mouse models compared to those consuming unmodified wheat bran, thus improving intestinal tolerance and probiotic function; the product can be used as a high-quality functional food ingredient.

The present invention provides a lyophilized composition containing tolvaptan sodium phosphate and a preparation method for the lyophilized composition. One or more of maltitol, raffinose, dextran, stachyose, arabinogalactan, fructooligosaccharide, galactooligosaccharide, isomaltooligosaccharide, polyethylene glycol, xylitol, or sorbitol is / are used as a lyoprotectant, so that the problem in the prior art that lyophilized compositions containing tolvaptan sodium phosphate are difficult to satisfy both high stability and high clarity; provided a tolvaptan sodium phosphate-containing composition which satisfies both high stability and high clarity, has a high dissolution rate and is easy to apply, reducing generation of impurities; in addition, in the preparation and long-term storage process, better stability is achieved, the opalescence and turbidity of liquid medicine during reconstitution and redissolution are avoided, dissolution is easier, and safe application of the product is facilitated.

The invention discloses a cactus external preparation and a preparation method thereof, and relates to the technical field of skin external preparations and preparation thereof.The cactus external preparation comprises, by mass, 4.5% of a cactus oligosaccharide compound, the molar ratio of mannan oligosaccharide to arabinogalactan in the cactus oligosaccharide compound is 3: 2, and the cactus oligosaccharide compound contains beta-1, 4 glucosidic bond modification groups; 6% of sericin temperature-sensitive microspheres, the particle size of the microspheres being 200-300 nm, and the phase transition temperature being 32-35 DEG C; according to the invention, staphylococcus epidermidis is activated to secrete antibacterial substances in a targeted manner through the cactus oligosaccharide compound, a physical barrier is formed in combination with the sericin temperature-sensitive microspheres, lactobacillus extracellular vesicles inhibit metabolism of propionibacterium acnes, glycerol caprylate selectively interferes with the fluidity of a pathogenic bacterium membrane, and konjac glucomannan regulates immunity; and a flora regulation and control-barrier protection-immune regulation system is constructed under the assistance of a pulsed electric field pretreatment process and the like, so that the problems of flora imbalance and drug resistance caused by traditional sterilization are solved, and skin micro-ecological balance and inflammation blocking are realized.

The invention discloses an anti-freezing and anti-oxidation compound agent for delaying quality deterioration of rinsing-reduced minced fillet in frozen storage and application of the anti-freezing and anti-oxidation compound agent, and belongs to the technical field of aquatic product processing. The invention relates to an anti-freezing and anti-oxidation compound agent for delaying quality deterioration of rinsing-reduced minced fillet in frozen storage. The anti-freezing and anti-oxidation compound agent comprises the following components in percentage by mass; the components comprise 30-40% of arabinogalactan, 0.005-0.01% of tea polyphenol and the balance of water; when in use, the arabinogalactan is dissolved in water, and the tea polyphenol is added after high-speed shearing treatment. The compound agent can reduce freezing denaturation caused by ice crystal formation and protein aggregation in the freezing storage period of rinsed surimi, reduce surimi quality deterioration caused by fat and protein oxidation, and effectively prolong the shelf life of the surimi.

A polysaccharide, or a composition thereof, wherein the polysaccharide comprises a backbone of beta-(1-6)-linked galactose residues and terminal residues, and wherein one or both of the following appl

Compositions, nutritional compositions and uses for modulating intestinal flora are provided. The composition for adjusting intestinal flora comprises the following components in parts by weight: 16-33% of fructo-oligosaccharide, 16-33% of arabinogalactan, 16-33% of a plant composition, 0-25% of inulin, 0-25% of galactooligosaccharide and 0-25% of chitosan oligosaccharide, and the plant composition comprises ginseng powder and a water extract prepared from the following raw materials in parts by weight: 1-10 parts of astragalus membranaceus, 1-10 parts of cinnamon, 1-10 parts of liquorice and 1-10 parts of fresh ginger. The weight ratio of the ginseng powder to the water extract is (50: 1)-(200: 1), and the weight ratio of the ginseng powder to the water extract is (50: 1)-(200: 1).

The invention belongs to the technical field of biological medicines, and particularly relates to a synthesis method of mycobacterium tuberculosis arabinogalactan 92-mer and a substructure thereof. Aiming at the technical problems of potential aglycone transfer and the like of an orthogonal one-pot glycosylation reaction strategy based on thioglycoside in the prior art, the mycobacterium tuberculosis arabinogalactan 92-mer and a substructure thereof are modularly and efficiently synthesized based on a one-pot glycosylation reaction strategy of glycosyl ortho (1-phenyl vinyl) benzoate (PVB).

The invention provides hyssopus harzianum polysaccharide as well as a preparation method and application thereof. The hyssop officinalis polysaccharide is prepared from galactose, arabinose and rhamnose. The molecular weight of the hyssop officinalis polysaccharide is 149 kDa, and the molar ratio of the galactose to the arabinose to the rhamnose is 59.59: 34.53: 5.88. The research clarifies that HCBP-0.1-W is arabinogalactan targeting TLR4 for the first time, and provides a theoretical basis for clinical application of HCBP-0.1-W as an immunologic adjuvant by activating dendritic cells and improving CTX-induced immunosuppression. Besides, by deeply researching the immunological enhancement effect of the hyssopus harzianum polysaccharide, the material basis of the aqueous extract of the hyssopus harzianum polysaccharide for treating related diseases can be clarified, and a new thought can be provided for development and utilization of the hyssopus harzianum.

The present invention relates to a composition comprising at least one oxazoline, such as OX100, for inhibiting the growth of Malassezia yeasts and / or for preventing and / or treating conditions caused by Malassezia yeasts, seborrheic dermatitis, cradle cap, Malassezia folliculitis, dandruff, Pityriasis versicolor or Pityriasis capitis. The present invention also relates to a composition comprising at least one oxazoline such as OX100, at least one compound such as malic acid diester and a vegetable oil oleodistillate and / or arabinogalactan, and to the use of same for preventing and / or treating, in particular, affections caused by Malassezia yeasts, seborrheic dermatitis, cradle cap, Malassezia folliculitis, dandruff, Pityriasis versicolor or Pityriasis capitis.

The invention provides a low-branched-chain beta-(1, 3)-D-galactan with high main chain bond content and a synergistic enzyme method preparation method thereof, and belongs to the technical field of preparation of glycans with specific structures. According to the method, larch arabinogalactan is used as a substrate, a compound enzyme system composed of beta-(1, 6)-D-galactanase and alpha-1, 3-arabinosidase from penicillium oxalicum is used for carrying out synergistic and directional enzymolysis on the larch arabinogalactan, so that beta-(1, 6)-D-galactose side chains and connected arabinose are effectively removed, and the yield of the larch arabinogalactan is increased. Thus, beta-1, 3-galactan with a complete main chain and obviously reduced branched chains is obtained. The low-branched-chain beta-(1, 3)-D-galactan prepared by the invention can be used in the fields of immunomodulators, functional food additives and the like, and has the effects of remarkably enhancing immunocompetence, improving intestinal health and the like.