27 results about "Stachyose" patented technology

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The application discloses a high-precision intelligent control and adjustment method for stachydrine fermentation and purification, and relates to the field of biological fermentation process control. The method comprises the following steps: obtaining a stachydrine mixed solution in a first sugar content interval; inoculating Eurotium cristatum at a first preset temperature after sterilization to start fermentation and obtain an initial fermentation liquor; monitoring the content ratio change of stachydrine and non-target sugars in real time through HPLC to obtain sugar content monitoring data; introducing a membrane separation device for selective dialysis to obtain membrane permeation rate monitoring data; performing dynamic evaluation on stachydrine enrichment according to the sugar content monitoring data and the membrane permeation rate monitoring data, generating a dynamic adjustment strategy for fermentation conditions based on the evaluation results; and performing fermentation process adjustment control. The method solves the technical problem that the existing stachydrine fermentation and purification cannot realize real-time and accurate monitoring and dynamic adjustment of fermentation conditions, resulting in low fermentation efficiency and low purity, and achieves the technical effect of improving the fermentation efficiency and purity of stachydrine through intelligent control of the fermentation process.

The invention discloses prebiotic soft fried dough twists and a making method thereof, and belongs to the technical field of food. The fried dough twist disclosed by the invention is prepared from the following raw materials in parts by mass: 132 to 169.5 parts of a dough base material, 5 to 6 parts of resistant dextrin, 7 to 9 parts of fructo-oligosaccharide microcapsules, 6 to 8 parts of isomaltitol, 1.5 to 2 parts of stachyose, 0.5 to 1.0 part of soybean lecithin and 0.01 to 0.03 part of vitamin E. The prebiotic retention rate is synergistically increased through microcapsule coating and a making process, the aging resistance of the fried dough twist is improved through synergistic moisturizing of resistant dextrin and fructo-oligosaccharide microcapsules, white granulated sugar is partially replaced by isomaltitol, stachyose and other sugar parts with low glycemic index, and the taste of the fried dough twist is improved. And the resistant dextrin with high dietary fiber content and the fructo-oligosaccharide prebiotics are matched, so that the product not only meets the sweet taste requirement, but also meets the healthy diet pursuit of modern consumers on low sugar, high fiber and high prebiotics.

The pet meat staple food grain based on composite prebiotic fermentation and the preparation method thereof have the advantages that 1, a composite prebiotic combination adopts a synergistic proportion of fructo-oligosaccharide, inulin, stachyose and galactooligosaccharide, and the regulation efficiency of intestinal flora is remarkably improved through the design that the proportion of a GF2 component is greater than or equal to 70% and the proportion of a long-chain component (the polymerization degree is 6-8) is greater than or equal to 20%; 2, animal meat sources (cattle tendon and chicken breast) and fermented plant raw materials are scientifically proportioned, and high-bioavailability protein and soluble fiber are provided; 3, the probiotic preparation is compounded by lactobacillus acidophilus and bifidobacterium in a ratio of 1: 1.5, and the survival rate of viable bacteria and the yield of metabolites are ensured in combination with a staged fermentation process; and 4, the mineral premix and the vitamin compound are accurately added, so that the nutritional requirements of pets in different life cycles are met, and the synergy of nutrition comprehensiveness and functionality is realized.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The invention discloses a prebiotic composition rich in dendrobium officinale polysaccharides. The prebiotic composition comprises the following components in parts by weight: 10-30 parts of dendrobium officinale polysaccharides, 4-28 parts of xylooligosaccharide, 5-20 parts of stachyose, 10-30 parts of galactooligosaccharide, 4-20 parts of psyllium husk powder and 10-30 parts of taste adjusting powder. According to the prebiotic composition rich in the dendrobium officinale polysaccharide, the efficient delivery and stability of the dendrobium officinale polysaccharide are ensured through the careful selection of the raw materials and the collaborative design of the formula, and the intestinal health promotion function is improved. The invention further provides a tablet candy adopting the prebiotic composition and a preparation method of the tablet candy.

The invention discloses a medicine for relieving side effects of gastrointestinal tracts caused by nintedanib, a medicine for preventing or treating pulmonary fibrosis and application of stachyose, and belongs to the technical field of medicines. The medicine taking the stachyose as the active ingredient can effectively relieve gastrointestinal tract side effects caused by the nintedanib, for example, intestinal mucosa lesions caused by the nintedanib can be obviously relieved, for example, intestinal mucosa tissue structure abnormity, intestinal cell proliferation activity reduction, intestinal cell apoptosis increase, intestinal tissue inflammatory factor gene expression level up-regulation and the like.

The application discloses a kind of soybean oligosaccharide content detection methods in food, the food is soy milk, the soybean oligosaccharide is stachyose, raffinose and sucrose.Method includes the following steps: (1) preparation standard stock solution and standard working solution;(2) preparation sample solution to be measured;(3) high performance liquid chromatography analysis condition;(4) establish standard working curve;(5) result analysis.The application establishes the high performance liquid chromatography determination method of low content level soybean oligosaccharide in soy milk.The application innovatively uses derivatization method to the soybean oligosaccharide (stachyose, raffinose and sucrose) and is derived, thereby greatly improves detection sensitivity, so that simple and easy to operate, without needing to carry out multiple large volume extraction solvent extraction and rotary evaporation concentration processing, and fills the blank of soybean oligosaccharide content detection field in soy milk.The method is simple in operation, high in sensitivity, good in reproducibility and stability, can satisfy the daily detection requirement of low content level soybean oligosaccharide in soy milk for inspection organization and enterprise internal laboratory.

The invention discloses a special extraction device for a stachyose production process, and relates to the field of stachyose preparation, the special extraction device comprises a drying cylinder, a feeding mechanism for spraying stachyose concentrate is arranged at the top in the drying cylinder, and a hot air mechanism is arranged at the bottom in the drying cylinder; a scraping mechanism is arranged in the drying cylinder and comprises an arc-shaped scraping plate attached to the inner wall of the drying cylinder, and the arc-shaped scraping plate is driven to move in a reciprocating mode so as to scrape away materials attached to the inner wall of the drying cylinder. And through the arranged arc-shaped scraping plate, materials (stachyose concentrate) attached to the inner wall of the drying cylinder can be scraped away, and the caking phenomenon caused by long-time drying is avoided.

The invention discloses moistening and smoothing composite probiotic powder and a preparation method thereof, and belongs to the technical field of functional foods. According to the probiotic powder, resistant dextrin and ultramicro-granulated psyllium husk powder are used as fiber matrixes, fried semen cassiae, fructus cannabis and radix polygonati officinalis are used as conditioning components, three prebiotics including fructo-oligosaccharide, galactooligosaccharide and stachyose are matched, and momordica grosvenori powder, freeze-dried mulberry powder and baked barley flour are used as auxiliary materials, so that the flavor and the taste are improved; the preparation method comprises the steps of low-temperature drying, 800-mesh superfine grinding, graded premixing, three-dimensional total mixing, whole grain screening and the like. According to the invention, the problems of easy caking, rough taste, heavy medicine flavor, poor solubility, strong irritation and the like of traditional like products are solved, and the obtained product is instant in water, fine and smooth, free of obvious medicine feeling, capable of mildly conditioning intestinal tracts and promoting smoothness, high in safety, suitable for long-term eating, stable in process and suitable for industrial production.

This invention discloses a gene combination, recombinant expression vector, construction method, and application for increasing the stachyose content of potatoes, belonging to the field of biotechnology. The gene combination includes the GalE gene, the GS gene, and the STS gene. Furthermore, this invention proposes a recombinant expression vector comprising the above gene combination. Additionally, this invention proposes a method for constructing the above recombinant expression vector, comprising: digesting the backbone vector with Eco31I enzyme, and ligating the target gene and regulatory element into the vector to form the recombinant expression vector pNK2-GalE-STS-GS. Furthermore, this invention proposes the application of the above gene combination, or the above recombinant expression vector, in increasing the stachyose content in potato tubers. The three genes GalE, GS, and STS proposed in this invention are specifically expressed in potato tubers, increasing the stachyose content in potato tubers by up to 16 times.

The present invention provides a spicy tricholoma matsutake leisure instant product and a preparation method thereof, the preparation method comprises the steps of raw material treatment, compound enzyme liquid spraying, pickling, magnetoelectric assisted pulse negative pressure permeation, frying, seasoning, packaging and sterilization, after blanching, the compound enzyme liquid containing papain and 5 '-phosphodiesterase is used for pretreatment of tricholoma matsutake, fibers are tenderized, and the delicate flavor is improved; then, a hollow multi-cavity liposome is added in the pickling link, a compact shell is formed by the liposome through yeast beta-glucan and stachyose, cannabis oil and purple sweet potato anthocyanin are wrapped in the compact shell, and flavor components and the functional liposome are promoted to deeply permeate into the tricholoma matsutake by utilizing a magnetoelectric auxiliary pulse negative pressure technology; the problems that a traditional product is uneven in flavor and functional components are prone to losing efficacy in the processing and digestion process are effectively solved, and finally the high-quality leisure instant product is obtained.

The present invention provides a lyophilized composition containing tolvaptan sodium phosphate and a preparation method for the lyophilized composition. One or more of maltitol, raffinose, dextran, stachyose, arabinogalactan, fructooligosaccharide, galactooligosaccharide, isomaltooligosaccharide, polyethylene glycol, xylitol, or sorbitol is / are used as a lyoprotectant, so that the problem in the prior art that lyophilized compositions containing tolvaptan sodium phosphate are difficult to satisfy both high stability and high clarity; provided a tolvaptan sodium phosphate-containing composition which satisfies both high stability and high clarity, has a high dissolution rate and is easy to apply, reducing generation of impurities; in addition, in the preparation and long-term storage process, better stability is achieved, the opalescence and turbidity of liquid medicine during reconstitution and redissolution are avoided, dissolution is easier, and safe application of the product is facilitated.

The application discloses an antioxidant prebiotic composition, a preparation method and application thereof, and relates to the technical field of functional food. The prebiotic composition according to the application is composed of the following components in parts by weight: 10-20 parts of plant complex enzyme stock solution, 1-10 parts of corn silk juice, 1-10 parts of winter gourd lotus leaf extract, 10-20 parts of polydextrose, 1-10 parts of stachyose, 1-10 parts of fructooligosaccharide, 1-10 parts of roxburgh rose powder, 1-10 parts of opuntia ficus-indica fruit juice powder, 1-10 parts of poria cocos powder, 1-10 parts of coix seed powder, 1-10 parts of Chinese yam powder, 1-10 parts of red bean powder and 1-5 parts of dried tangerine or orange peel powder. The plant enzyme, prebiotic and specific food components are selected and prepared into the composition in a specific proportion, so that the antioxidant effect can be achieved.

The invention discloses anti-freezing potato modified starch and a preparation method and application thereof, and the preparation method of the starch comprises the following steps: adding stachyose into potato starch, uniformly mixing, adding water to adjust the water content to 40-50%, treating with an extrusion bulking machine, and finally drying and crushing to obtain the anti-freezing potato modified starch. After the anti-freezing potato modified starch is frozen for five times, the water separation rate is 62.61 to 74.93 percent. The potato starch and the stachyose are subjected to extrusion treatment to improve the freeze-thaw stability of the potato starch, the problems that natural potato starch is high in bleeding rate, insufficient in solubility and expansion degree and low in digestion resistance are solved, and the potato starch is applied to fresh dumpling wrappers to improve the freezing resistance and sensory characteristics of the dumpling wrappers.

The invention relates to the technical field of biological pharmacy, in particular to application of a rhizoma polygonati extract in preparation of a medicine for treating liver injury, and the rhizoma polygonati extract is prepared from the following main active ingredients: hexadecanamide, L-valine, 2-pyrrolidine carboxylic acid, 4-oxoproline, manninotriose, succinic acid, choline, D-(-)-ribose, stachyose and L-threonine. The invention proves that the prophylactic administration of the rhizoma polygonati extract can improve the acetaminophen-induced liver injury, so that the rhizoma polygonati extract has important clinical significance on pain patients who need to take acetaminophen for a long time, and can be developed into an effective liver protection auxiliary medicine.

The invention relates to the technical field of beer preparation, in particular to high-activity probiotic soybean whey beer and a preparation method thereof, and aims to increase the viable count of probiotics in beer. The preparation method comprises the following steps: fermenting lactic acid bacteria in soybean whey to prepare soybean whey probiotic bacteria base liquid, performing maltolization and boiling wort; and carrying out primary fermentation on the wort, adding the soybean whey and the soybean whey probiotic base solution, and carrying out secondary fermentation to obtain the soybean whey craft beer containing high-activity probiotics. According to the invention, the competitive inhibition effect of saccharomycetes on lactic acid bacteria is reduced through the domestication growth and activity protection effect of the soybean whey on the lactic acid bacteria, so that the activity of probiotics in the beer is fully retained, and the beer has a fermented mellow flavor, also contains rich viable bacteria nutrition and active substances such as leucine, raffinose and stachyose, and has a good taste. The effect of relieving the burdens after drinking is exerted.

The present application relates to the technical field of normal temperature fruit tea beverage, and specifically provides a zero-sugar and zero-fat functional fruit tea beverage and a preparation method thereof. The mass ratio of soluble soybean polysaccharide to Luo-shen oolong tea liquid in the zero-sugar and zero-fat functional fruit tea beverage is (0.5-2):(300-400). The sweetening agent in the zero-sugar and zero-fat functional fruit tea beverage is composed of erythritol, xylitol, stachyose, sucralose and acesulfame potassium. The optimal stabilizer is screened out by testing different stabilizer raw materials, and the sweetening agent combination which does not affect the stability of the product is screened out, so that the system stability problems such as precipitation, water separation and particle floating during the shelf life of the product are improved, and the product maintains high stability. The product provided by the present application has pure sweetness, does not add food essence, and the sweetness does not have the artificial feeling of lengthening.

The invention relates to an intestine-moistening and bowel-relaxing composition capable of being used for preparing popping beads. The intestine-moistening and bowel-relaxing composition comprises one or a combination of more of psyllium seed, fig pulp, apple pulp, lemon juice, polyxylose, stachyose, jujube and astragalus membranaceus. The popping bead is prepared on the basis of food and medicine substances, and the popping bead is relatively simple in preparation process, relatively low in cost and suitable for large-scale production. The obtained popping beads are good in taste and wide in application, can be directly eaten, and can also be used as an auxiliary material to be added into various beverages popular in the market, such as milk tea, coconut juice, fruit tea and the like. In the function, the popping bead can increase and regulate the water content of excrement, promote intestinal tract movement, shorten the defecation time and effectively relieve constipation; and moreover, the popping bead does not contain powerful laxative, so that the popping bead is prevented from injuring the body, and is high in safety and suitable for long-term consumption of consumers.

This invention relates to the field of biotechnology, specifically to a high-yield α-galactosidase-producing mutant strain of *Weizmannii coagulans* and its application. This invention is the first to propose using *Weizmannii coagulans* as an α-galactosidase-producing bacterium. Through mutagenesis, a high-yield α-galactosidase-producing mutant strain was obtained. The fermentation broth of the mutant strain showed a 4.37-fold increase in α-galactosidase activity compared to the original strain, maintaining over 60% activity within a pH range of 6.0–10.0, and exhibiting 1.80 times better heat resistance than the original enzyme. Furthermore, it can hydrolyze the anti-nutritional factors mesenteryne, raffinose, and stachyose in soybean meal, demonstrating high industrial value in the fermented soybean meal and feed processing industries. This invention lays the foundation for the application of *Weizmannii coagulans* in the manufacturing and processing of α-galactosidase.

The invention discloses a proliferation probiotic composition as well as a preparation method and application thereof, and belongs to the technical field of probiotics. In order to solve the technical problem that an existing probiotic agent is poor in proliferation effect on bifidobacteria and lactobacillus, the invention provides the composition which is prepared from the following components in parts by weight: 1-90 parts of prebiotics, 0.1-20 parts of peptide and 1-20 parts of plant extract and has a remarkable probiotic proliferation effect; the prebiotics are selected from one or more of inulin, stachyose, fructo-oligosaccharide, galactooligosaccharide and isomaltooligosaccharide; the peptide is selected from one or more of casein hydrolysis peptide, collagen peptide and egg white peptide; the plant extract is selected from one or more of euglena powder, fucoidin powder and mushroom powder; the probiotics comprise bifidobacterium or lactobacillus.

The application discloses a kind of alpha-galactosidase mutants with high specific activity and thermal stability and application thereof.The alpha-galactosidase AgaV derived from Anoxybacillus vitaminiphilus is used as a template, and amino acid mutation is carried out by using molecular biology techniques to obtain a kind of mutant with improved catalytic efficiency, including Y340E, N549Q and T550N.The mutant has high catalytic efficiency and strong thermal stability, and the hydrolysis efficiency of rfo, such as raffinose and stachyose, is increased by 4.0-6.0 times, and has a wide application prospect for hydrolysis and removal of rfo in soybean products.

The invention relates to the field of food, in particular to a chicken skin fruity macadamia nut fermented beverage and a preparation method thereof. The preparation method comprises the following steps: (1) mixing macadamia nut pulp with water, and sequentially juicing and filtering to obtain nut pulp; (2) sequentially performing astringency removal, soaking and juicing on the clausena lansium fruits, performing primary filtration on the squeezed fresh juice stock solution with water, performing enzymolysis on the filtrate, and performing filtration again to obtain clausena lansium fruit juice; (3) mixing the chicken skin juice, the white granulated sugar, the stachyose and the nut pulp, and sterilizing to obtain a mixed material; and (4) inoculating lactobacillus plantarum into the mixed material, fermenting, and sterilizing to obtain the chicken skin fruity macadamia nut compound fermented beverage. The obtained product has macadamia nut fragrance and fermentation flavor, is moderate in sour and sweet, appropriate in taste and relatively stable in quality, and meets the requirements of consumers.

The invention discloses an intestinal micro-ecology appetite regulator, which comprises an intestinal micro-ecology regulator and an appetite regulator, the weight ratio of the intestinal micro-ecology regulator to the appetite regulator is 1: 1, and the intestinal micro-ecology regulator comprises the following components: mannose oligosaccharide, stachyose, xylooligosaccharide and raffinose. The appetite regulator comprises the following components: a white kidney bean extract, blueberry powder, mung bean coix seed powder and ganoderma lucidum spore powder; compared with the prior art, the traditional Chinese medicine composition has the advantages of being capable of regulating intestinal microecology, regulating appetite and controlling body weight.

The application discloses a kind of fermented compound feed for preventing piglet diarrhea and its preparation and application method. Corn, soybean meal and bran are premixed according to the mass ratio of 4.5:4.5:1.0, then 10% of ZJUAF-2 fermentation broth and 10% of ZJUAF-4 fermentation broth are added, and 40% of water is added. The initial fermentation temperature is 25-30℃, the fermentation time is 48-72 h, and the material is turned over every 5-8 h. When the pH of the feed is reduced to 4.0, corn-soybean meal type fermented compound feed is obtained. Corn, soybean meal and bran are fermented by mixing one strain of Bacillus subtilis and one strain of Pediococcus pentosaceus. The contents of beta-conglycin and soybean protein decreased by 71.94% and 82.86%, respectively. The content of neutral detergent fiber decreased by 38.93%. The contents of gassy factors raffinose and stachyose decreased by 42.22% and 36.60%, respectively. The content of phytic acid phosphorus decreased by 37.50%. In addition to the significant decrease in the above-mentioned anti-nutritional factors, the lactic acid content increased by 3.5 times, and the acid-soluble protein increased by 6 times. Feeding the fermented feed to piglets can control diarrhea as effectively as feeding antibiotics.

The application provides a preparation method of stachydrine, and belongs to the technical field of stachydrine; the method comprises the steps of pretreatment, extrusion puffing, leaching, pressure filtration, denaturation and separation of residual protein, concentration, desalting and purification, refining, concentration and drying; in the extrusion puffing step, the pretreated soybean meal is extruded for 40-60s by a double-screw extruder, the pressure of the double-screw extruder is 1.0-1.5 MPa, and the temperature is 135-165 DEG C, so that the puffed material is obtained; the stachydrine obtained by the preparation method has a purity of 98.2-99.50%, low salt content, low conductivity and low colority.

The invention relates to the technical field of food engineering, and discloses a stachyose and Chinese yam beverage composition capable of invigorating spleen, benefiting stomach and conditioning constipation and diarrhea and a preparation method thereof.The stachyose and Chinese yam beverage composition is prepared from 22-30 parts of Chinese yam raw materials, 6-12 parts of whole milk powder, 3-5 parts of stachyose, 1-2.5 parts of sweetening agents, 0.5-1.2 parts of acidity regulators, 0.1-0.4 part of edible essence and 40-55 parts of water. The final total protein content of the beverage composition is greater than or equal to 1.0 g / 100g, and the proportion of the plant protein from the Chinese yam in the total protein is greater than or equal to 51%. The Chinese yam and stachyose are taken as core compound components, Chinese yam vegetable protein and mucus polysaccharide can enhance the digestion and absorption functions of the spleen and the stomach, stachyose can effectively promote the proliferation of beneficial bacteria in the intestinal tract, and the problems of dyspepsia caused by weakness of the spleen and the stomach and constipation and diarrhea caused by imbalance of intestinal flora are bidirectionally improved; meanwhile, whole milk powder is matched to supplement milk protein, a conditioning and nutrition synergistic system is formed, the health requirements of different crowds are met, all functional components are natural sources, and the acceptability of consumers is high.