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Stachyose is a tetrasaccharide consisting of two α-D-galactose units, one α-D-glucose unit, and one β-D-fructose unit sequentially linked as gal(α1→6)gal(α1→6)glc(α1↔2β)fru. Together with related oligosaccharides such as raffinose, Stachyose occurs naturally in numerous vegetables (e.g. green beans, soybeans and other beans) and other plants.
Mutant strain of weizmannia cohnii with high yield of alpha-galactosidase and application thereof
This invention relates to the field of biotechnology, specifically to a high-yield α-galactosidase-producing mutant strain of *Weizmannii coagulans* and its application. This invention is the first to propose using *Weizmannii coagulans* as an α-galactosidase-producing bacterium. Through mutagenesis, a high-yield α-galactosidase-producing mutant strain was obtained. The fermentation broth of the mutant strain showed a 4.37-fold increase in α-galactosidase activity compared to the original strain, maintaining over 60% activity within a pH range of 6.0–10.0, and exhibiting 1.80 times better heat resistance than the original enzyme. Furthermore, it can hydrolyze the anti-nutritional factors mesenteryne, raffinose, and stachyose in soybean meal, demonstrating high industrial value in the fermented soybean meal and feed processing industries. This invention lays the foundation for the application of *Weizmannii coagulans* in the manufacturing and processing of α-galactosidase.
Owner:KUNMING QACTIVE BIOLOGICAL TECH CO LTD