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Method for efficiently preparing stachyose from stachys sieboldii

A technology of stachyose and caddis, which is applied in the field of high-efficiency preparation of functional oligosaccharides, can solve the problems of insufficient utilization of waste liquid, limited production efficiency, and high production costs, so as to reduce salt pollution and improve Quality, the effect of saving production costs

Active Publication Date: 2010-06-02
上海国矗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, at present, many domestic stachyose production enterprises still have deficiencies in the production process. Although the purity and extraction rate of stachyose extracted from soybeans have been greatly improved, the production process consumes a lot of energy and the waste liquid in production Not fully utilized, limited production efficiency, high production costs

Method used

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  • Method for efficiently preparing stachyose from stachys sieboldii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 3 parts of fresh caddis silkworm, 100 kg each, and add 1000 kg of deionized water according to the ratio of material to liquid (w / v) 1:10, and compare the extraction rates of different extraction methods.

[0016] (1) Homogenization extraction method: the temperature is room temperature, the rotation speed is 30000rpm, and the homogenization time is 10min;

[0017] (2) Enzymatic hydrolysis method: the ratio of enzyme solution is 1.0% cellulase, 1.0% pectinase, 0.8% protease, the pH value of enzymolysis is 5.5, the enzymolysis time is 100min, and the enzymolysis temperature is 45°C;

[0018] (3) Blank control group: extract at 45°C for 100 minutes;

[0019] The extracts obtained by the above three extraction methods were sampled respectively, and the stachyose content was determined by HPLC-RID method, and the extraction rates of the three methods were 83%, 76%, and 45%, respectively.

[0020] It can be seen from Example 1 that the homogenate extraction method has ...

Embodiment 2

[0022] (1) Extraction of stachyose from homogenate: Take 100 kg of fresh caddis silkworm, add 500 kg of deionized water according to the ratio of material to liquid (w / v) 1:5, and homogenate for 15 minutes at a temperature of 30°C and a speed of 22,000 rpm. Efficient extraction of stachyose, the homogenate sample was detected by HPLC-RID method, and the extraction rate of stachyose reached 82%;

[0023] (2) Filtration: Slag material is separated by cloth bag filtration. Filtration area 5m 2 , filter pore size 5um, filter pressure 0.5Mpa, water flow rate 5T / h, 537 kg of filtrate was obtained after the extract was separated from slag.

[0024] (3) Macroporous resin decolorization: at normal temperature, pass the filtrate through a chromatographic column equipped with macroporous adsorbent resin at a flow rate of 2 column bed volumes / hour for decolorization, and stop loading when the processing capacity is 3 times the column bed volume , with 2 times of column bed volume top wa...

Embodiment 3

[0027] (1) Homogenate to extract stachyose: take 100 kg of fresh caddis silkworm, add 1500 kg of deionized water according to the ratio of material to liquid (w / v) 1:15, and homogenate for 25 minutes at a temperature of 30 °C and a speed of 10000 rpm. , the homogenate was detected by HPLC-RID method and the extraction rate of stachyose was 83.7%;

[0028] (2) Cloth bag filter: filter area 7m 2 , filter aperture 5um, filter pressure 0.3Mpa, water flow 10T / h. Obtain 1570 kilograms of filtrates after the slag material is separated;

[0029] (3) Decolorization of macroporous resin: at normal temperature, the filtrate is decolorized through a chromatographic column equipped with macroporous adsorbent resin at a flow rate of 2 column bed volumes / hour, and the sample loading is stopped when the processing capacity is 5 times of column bed volume , with 3 times the column bed volume top wash with deionized water, to get 1790 kg of decolorization solution;

[0030] (4) Ultrafiltrati...

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Abstract

The invention provides a method for efficiently preparing stachyose from stachys sieboldii. The method is characterized by comprising the following steps: taking Chinese traditional vegetable stachys sieboldii as a raw material; extracting the stachyose by adopting a pure water homogenate method; and spraying and drying after the processes of filtering a homogenate by a cloth bag; decolorizing the filtered homogenate with macroporous resin, performing ultrafiltration purification and reverse osmosis concentration, and the like, wherein a reverse osmosis permeate liquid is used as water for the homogenate and the resin decolonization elution in the production and the like, so not only resources can be used fully to meet the requirement on production water supply, but also the influence on the purity of the stachyose in the whole production system, caused by pollutants such as saline matters and the like in a natural water source, can be reduced to further improve the purity and the quality of the stachyose. By using the method of the invention, the extraction rate of the stachyose prepared can reach 75 and 85 percent, the purity of the stachyose in a product reaches over 90 percent, and the total recovery rate of the stachyose in the whole process reaches over 60 percent. The method combines a traditional extraction method and a modern separation method, has a simple process and a large handling capacity, is suitable for industrial production, and simultaneously has high product purity, saves resources and the production cost, and has good economic benefits and ecological benefits.

Description

technical field [0001] The invention relates to a high-efficiency preparation technology of functional oligosaccharides, in particular to the technical field of extracting and purifying food functional factors from traditional Chinese plants. Background technique [0002] Stachyose is a naturally occurring tetrasaccharide, belonging to the raffinose genus galactoside non-reducing functional oligosaccharides. It is composed of one molecule of glucose, one molecule of fructose, and two molecules of galactose. The structural formula is Fructose-glucose-galactose-galactose, molecular formula C 24 h 42 o 2 , the molecular weight is 666.59. Since the glucose group in the molecule and the two galactoses are connected by -D-galactosidic bonds, and the human body lacks α-D-galactosidase, stachyose cannot be digested and absorbed by the human body, and only intestinal A few beneficial bacteria such as Bifidobacterium and Lactobacillus acidophilus in the tract have an enzyme system...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08B01D61/02B01D61/14
Inventor 王洪新姚红朱松
Owner 上海国矗生物科技有限公司
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