The invention discloses a hand cleaner composition. The hand cleaner composition comprises saccharide prebiotics, a preservative, a surfactant and water, wherein the ratio of the mass of the saccharide prebiotics to the mass of the preservative to the mass of the surfactant is (1-15):(0.1-2):(1-20); the saccharide prebiotics is at least one of polyfructosan, galacto-oligosaccharide, lactulose and raffinose; the preservative is at least one of nipagin ester, sodium nipagin acid ester, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, dehydroacetic acid, sodium dehydroacetate, benzyl alcohol and phenoxyethanol; and the surfactant is one of a nonionic surfactant, an anionic surfactant and an ampholytic surfactant. The hand cleaner composition can be used for rubbing hands without water, and has a good cleaning effect; and in addition, the saccharide prebiotics can effectively replace absorption fungus microorganisms on the surface of skin, so that the probability that bacteria directly attack the skin is eliminated.