30 results about "Raffinose" patented technology

The application provides a cell fixing solution for a myasthenia gravis antibody spectrum (IgG) detection kit (cellular immunofluorescence method) and a product and application thereof, and relates to the technical field of in-vitro detection. The cell fixing solution provided by the application is composed of polyoxymethylene, formaldehyde, acetic acid, raffinose and a buffer solution, can effectively fix the cell morphology, maximally retains the natural spatial conformation and antigen epitope integrity of myasthenia gravis related antigens AChR, Titin, RyR1, MuSK and LRP4, reduces the problems of antigen denaturation, epitope shielding and protein loss in the fixing process, can efficiently and accurately realize the synchronous joint detection of various specific antibodies, and the myasthenia gravis antibody spectrum (IgG) detection kit (cellular immunofluorescence method) prepared by the application has stable detection results, and has important clinical application value and industrial popularization prospect.

The invention provides an NKT cell preparation as well as a preparation method and application thereof, and belongs to the field of biomedical treatment. The NKT cell preparation provided by the invention comprises NKT cells, a compound electrolyte injection, 5-10% of human serum albumin, 5-10% of raffinose and 2-5% of lysine, wherein the concentration of the NKT cells in the cell preparation is 1.0 * 10 < 6 >-1.0 * 10 < 8 > / mL; the prepared finished product preparation is long in shelf life, can be stably stored at 4 DEG C for more than 144 hours, and can be stably stored at-80 DEG C for at least 12 months.

This invention provides the preparation and application of a strain of *Lactiplantibacillus plantarum* INM3104 that reduces raffinose in whole grain dough. This strain, *Lactiplantibacillus plantarum* INM3104, which degrades raffinose, was deposited at the Guangdong Provincial Microbial Culture Collection Center on April 11, 2024, with accession number GDMCC NO:64512. The advantages are: it only reduces the raffinose content in whole grain products, while also improving the flavor and quality of the whole grain products; the whole grain products produced using this preparation do not affect the overall processing of the whole grain products after fermentation, not only improving the taste of the whole grain products but also reducing the adverse factors associated with whole grain products.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The present invention provides a use of a GAL-5B protein and a coding gene therefor in plant breeding, wherein the loss of function of the GAL-5B protein can significantly promote the accumulation of raffinose in plant seeds, while simultaneously increasing the protein content and reducing the starch content in the seeds; moreover, overexpression of GAL-5B reduces the raffinose content in the plant seeds.

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.

The invention discloses a sucrase mutant, an expression strain of the sucrase mutant and application of the sucrase mutant in hydrolysis of carbohydrates in soybean milk, and belongs to the technical field of biology. The sucrase mutant disclosed by the invention is simply recorded as InvDz13-TBT-01, and the amino acid sequence of the sucrase mutant is shown as SEQ ID NO: 2. The sucrase mutant InvDz13-TBT-01 is subjected to heterologous expression in bacillus subtilis, a recombinant strain is obtained, and the specific enzyme activity and the stability of protein produced by fermentation of the recombinant strain are remarkably improved. When sucrose is used as a substrate, the specific enzyme activity of the mutant enzyme is improved by 1.94 times, and when raffinose is used as a substrate, the specific enzyme activity of the mutant enzyme is improved by 1.7 times. Under the condition of 35 DEG C, the stability of the mutant enzyme is improved by 4 times. Compared with the starting enzyme, the thermal stability of the mutant enzyme is greatly improved while the specific enzyme activity is maintained. The sucrase mutant disclosed by the invention has a potential value in the application of hydrolyzing carbohydrates such as raffinose in soybean milk.

The invention discloses application of a soybean GmCDF3 gene in regulating and controlling the content of seed protein, amino acid and raffinose, and relates to application of soybean GmCDF3. According to the application of the soybean GmCDF3 gene in regulating and controlling the contents of seed protein, amino acid and raffinose, the application method is to culture plant seeds by using the soybean GmCDF3 gene, and further improve the plant quality. According to the invention, a GmCDF3 overexpression vector is constructed by utilizing a molecular means, and the soybean GmCDF3 gene is overexpressed in soybean through an agrobacterium-mediated genetic transformation technology, so that the content of 12 amino acids such as cysteine, leucine, lysine, phenylalanine and threonine in soybean seeds can be remarkably increased, and the yield of soybean seeds is increased. The protein content of the seeds is obviously improved, the oil content and the grain weight are not influenced, and the raffinose content is obviously reduced. Meanwhile, the soybean GmCDF3 gene is over-expressed in arabidopsis thaliana, and the protein content of arabidopsis thaliana seeds is also remarkably increased.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The invention discloses a cottonseed protein solubility improving method based on pH shift synergistic homologous raffinose compounding, and relates to the technical field of plant protein modification. The method for improving the solubility of the cottonseed protein comprises the following steps: uniformly mixing raffinose, the cottonseed protein and water, carrying out alkaline pH (Potential of Hydrogen) migration treatment, centrifuging to obtain supernate, and dialyzing the supernate to obtain a cottonseed protein-raffinose compound. According to the method, an alkaline pH shift-raffinose in-situ compounding synergistic strategy is put forward for the first time, the low molecular weight characteristic of the cottonseed byproduct raffinose is utilized, the raffinose is accurately embedded into a cottonseed protein hydrophobic structural domain, reaggregation of the protein is blocked through the dual effects of hydrogen bond combination and electrostatic repulsion, and breakthrough improvement of the solubility of the cottonseed protein is achieved.

The invention relates to the technical field of biology, and discloses plant lactobacillus IMAU17175 and application of the plant lactobacillus IMAU17175 in fermented soybean milk. The lactobacillus plantarum IMAU17175 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36568, the preservation time is November 11, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the lactobacillus plantarum is classified and named as lactobacillus plantarum. The fermented soybean milk is prepared by adopting the plant lactobacillus IMAU17175 in cooperation with the streptococcus thermophilus S10, and the plant lactobacillus IMAU17175 has the in-vitro characteristics of metabolism of D-raffinose and cholesterol, so that the final product is rich in active functional strains while the flatulence factor content is reduced.

The application provides a kit for detecting sialylated carbohydrate antigen KL-6 and a use method and application thereof, and relates to the field of in vitro detection. The kit for detecting sialylated carbohydrate antigen KL-6 provided by the application comprises magnetic beads, KL-6 antibodies, acridinium ester, a magnetic bead storage solution, an acridinium ester storage solution, a sample processing solution, a pre-priming solution and a priming solution. The sample processing solution comprises, in terms of mass fraction, 0.03-0.3% polyoxyethylene fatty acid ester, 0.3-2.0% bovine serum albumin, 0.3-2.0% raffinose, 0.3%-2.0% sodium hydroxide, 0.01-1.5% NaN3 and 0.3-1.5% sodium chloride, the rest is purified water, and the pH is 8-10. The kit can be used for determining sialylated carbohydrate antigen KL-6 in serum, and has high accuracy and strong anti-interference ability.

The application provides a serum-free cell cryopreservation solution for epidermal stem cells and a preparation method and application thereof, and specifically belongs to the technical field of stem cell cryopreservation.The serum-free cell cryopreservation solution for epidermal stem cells comprises the following components in the following amounts: 0 g / L to 50 g / L raffinose, 3 g / L to 30 g / L dextran, 0% to 30% (volume percent) glycerol, 0% to 10% (volume percent) DMSO, 0% to 30% (volume percent) platelet lysate, and the balance of Ham's F-12; wherein the values of DMSO and glycerol are not both 0%. The serum-free cell cryopreservation solution can significantly improve the proliferation activity and colony formation ability of epidermal stem cells after resuscitation.

The present application relates to the field of nanotechnology, in particular to a raffinose-SS31 peptide multi-targeted functional chitooligosaccharide / astaxanthin nanoparticle and a preparation method and application thereof.The nanoparticle of the present application takes precise regulation at the nanometer scale, innovative application of new components and targeted synergistic delivery as the core design idea, constructs a multifunctional nanofunctional network, provides support for the nanoscale precise delivery of active ingredients such as astaxanthin, and at the same time, establishes a chitooligosaccharide nanoparticle preparation technology with high active substance loading rate and stability for the problems of low stability and poor efficacy of chitooligosaccharide encapsulated active substances;targeting ligands are introduced by structure modification to enhance the specific recognition and binding capacity of nanoparticles to specific cells and tissues, and a targeted delivery technology for site-specific release and precise delivery of active substances is established.

This invention discloses an in-situ colonic short-chain fatty acid production promoter, its preparation method, and its application. The preparation method includes: Step 1, reaction system preparation: Raffinose is added to distilled water and stirred at 50-60°C until completely dissolved, preparing a raffinose aqueous solution with a mass-volume concentration of 20-40%. The pH of the solution is adjusted to 8-9 to obtain an alkaline reaction system; Step 2, esterification reaction: Maintaining the system at 50-60°C and pH 8-9, butyric anhydride is slowly added to the alkaline reaction system. After the addition is complete, the reaction is maintained at this temperature, with the total reaction time controlled at 1-6 hours; after the reaction is completed, the pH of the system is adjusted to 6-7 to terminate the reaction, obtaining an esterification reaction solution; Step 3, post-processing: The esterification reaction solution is cooled to room temperature and successively concentrated, purified, and lyophilized to obtain the target product. The beneficial effects of this invention are: it can comprehensively improve the level of short-chain fatty acids in the colon and optimize the intestinal flora structure, breaking through the bottleneck of short-chain fatty acid delivery.

Presented herein is a galactooligosaccharide characterized by a high raffinose content and a method for its production.

The invention discloses a material supplementing method of saccharomyces cerevisiae high-density fermentation recombinant collagen of a galactose promoter, which comprises the following steps: after fermentation culture for 24 hours, starvation culture is carried out to consume up glucose when the OD (optical density) of fermentation liquor reaches 100, then an inducer is added into a fermentation tank for induction, and fructose is added during induction. According to the method, fructose is supplemented as a main carbon source when galactose is added for induction, the method has the advantages of higher strain activity, higher expression yield, higher saccharomyces cerevisiae fermentation density and the like compared with a method without fructose, the price of fructose is lower than that of glucose and extremely lower than that of raffinose, and the main carbon source supplemented with fructose for induction has a remarkable economic effect.

The application provides application of a CsABF8 gene in regulation of drought resistance of plants and belongs to the technical field of molecular breeding.The application takes a drought-sensitive variety 'Fuyun No.6' ('FY') and a drought-tolerant variety 'Taicha No.12' ('TC') as experimental materials, performs drought stress treatment and non-targeted metabolome screening of differential metabolites, and determines that the CsABF8 gene is a potential drought-resistant target gene.The CsABF8 gene can respond to drought stress, increases the content of raffinose in tea trees by regulating CsGolS1, CsGolS2 and CsRaf6 promoters in the tea trees, and thus makes the tea trees cope with drought stress.

The invention discloses a fluorescent quantitative PCR freeze-drying protection composition, a freeze-drying ball, a preparation method, a kit and application. The composition comprises the following components in parts by weight: 1 to 5 parts by weight of glucan, 0.01 to 1 part by weight of bovine serum albumin, 1 to 5 parts by weight of raffinose, 1 to 3 parts by weight of polyethylene glycol, 0.01 to 2 parts by weight of arginine, 0.01 to 2 parts by weight of polysucrose and 2 to 10 parts by weight of trehalose. The weight-average molecular weight of the glucan ranges from 30000 Da to 50000 Da; the weight-average molecular weight of the polyethylene glycol is 20000 Da to 40000 Da; and the weight-average molecular weight of the polysucrose ranges from 60000 Da to 80000 Da. According to the fluorescent quantitative PCR freeze-drying protection composition, through the synergistic effect of the components, the enzyme activity is maintained to the maximum extent, and a freeze-drying reagent is endowed with excellent thermal stability and long-term storage performance.

The invention discloses an application of gossypose in improving freezing resistance of beet, and belongs to the technical field of plant freezing stress. The purpose of the invention is to improve the freezing resistance of beet. A method for resisting freezing stress of beet comprises the following steps: spraying a raffinose solution at the external source of beet seedlings in an environment of-4 DEG C, and culturing the beet seedlings. An effective method is provided for improving the freezing resistance of the beet.

This invention discloses a porcine lactobacillus mucosa HEB-FEC-LM-001 and its applications, belonging to the field of feed additive technology. The porcine lactobacillus mucosa HEB-FEC-LM-001 provided by this invention is a Gram-positive bacterium with a milky-white, raised colony appearance, clear edges, and a smooth surface. It was deposited at the China Center for Type Culture Collection on October 21, 2025, with accession number CCTCC NO:M 20252272. A synbiotic is prepared by combining porcine lactobacillus mucosa HEB-FEC-LM-001 with raffinose. When fed as a feed additive to weaned piglets, it can significantly improve the piglets' intestinal health, apparent nutrient digestibility, and immune function, significantly reduce the diarrhea rate, and improve the growth performance of weaned piglets. Therefore, it has broad application prospects as a feed additive for weaned piglets.

This invention belongs to the fields of molecular biology, immunology, and virology. It discloses a microfluidic detection kit and method for beet western yellow virus based on RT-LAMP-CRISPR / Cas12b. This invention utilizes a raffinose dynamic diffusion system to connect isothermal amplification LAMP and CRISPR / Cas12b detection in a single reaction chamber, and achieves simultaneous detection of multiple samples through a microfluidic chip. This detection method effectively avoids aerosol contamination, possesses excellent specificity, sensitivity, and visualization advantages, is simple to operate, requires no expensive instruments, and can easily and quickly detect whether pepper and beet samples are infected with beet western yellow virus in the field, improving detection efficiency.

The invention discloses a method for improving black spot resistance of crops by raffinose, which comprises the following steps: selecting healthy and consistent seedling-stage plants of the crops, and culturing; raffinose solution preparation: preparing raffinose solutions with different concentrations, respectively spraying the raffinose solutions on the plants in the step 1, spraying the raffinose solutions once every 24 hours in a laboratory scene, spraying the raffinose solutions twice in total, spraying the raffinose solutions once every 5 days in a field scene, spraying the raffinose solutions three times in total, and spraying clear water in a contrast manner; after spraying in the step 2 is finished for 24 hours, inoculating alternaria alternata to the control plants and the treated plants, comparing disease phenotypes under different raffinose concentrations, and screening the concentration with the optimal effect; 3, according to the result of the step 3, determining that the spraying effect of the raffinose solution with the concentration of 2.4% is optimal, selecting the raffinose solution with the concentration to be sprayed on the plants, the spraying method is the same as that of the step 2, performing artificial inoculation 24 hours after the last time of spraying is finished, observing the disease phenotype, and counting the disease state. After being treated by the 2.4% raffinose solution, the disease spot areas of the tea chrysanthemum, the apples and the tomatoes are obviously reduced.

The application provides a cell climbing sheet processing reagent for a Ranvier node / paranode antibody spectrum detection kit (cellular immunofluorescence method), a method and application thereof, and relates to the technical field of in-vitro detection. The cell climbing sheet processing reagent comprises a cell fixing solution with a specific composition and an antigen repair composition after cell fixing, wherein the cell fixing solution is composed of paraformaldehyde, formaldehyde, acetic acid, raffinose and a buffer solution, and the antigen repair composition after cell fixing is composed of ammonium sulfate, hydroxylamine hydrochloride, Brij 78, TCEP, ProClin 300 and a buffer solution. Through the synergistic effect of the two, the natural conformation of the target antigen is maximally retained, and non-specific interference is reduced, so that precise and efficient detection of the Ranvier node / paranode antibody spectrum NF155, NF186, CNTN1, CASPR1 and MAG can be realized, the clinical diagnosis demand is met, and reliable technical support is provided for precise typing of autoimmune Ranvier node disease.

The present application provides a probiotic preparation and its use. The probiotic preparation of the present application comprises active ingredients and adjuvants, wherein the active ingredients comprise oxygen scavengers, organic acid producers and place holders; the adjuvants comprise flavoring agents, oligosaccharides and other additives; wherein the oligosaccharides are not lactulose, stachyose, raffinose, oligogentiobiose and oligochitosan, and the other additives are not theaflavins, ethylenediaminetetraacetic acid disodium salt and sorbic acid. The present application effectively promotes the metabolic activity of probiotics through a specific combination of adjuvants, rather than just providing physical protection or nutritional support. Experimental data show that the adjuvant combination of the present application can significantly and continuously improve the oxidation-reduction potential and Na + K + ATPase activity of probiotics. Moreover, the probiotic preparation of the present application can effectively improve functional gastrointestinal diseases and has a bidirectional regulation function, which is suitable for different intestinal motility disorders, such as diarrhea and constipation.

The invention discloses a preparation method and application of an ardealite geopolymer with retarding and anti-cracking enhancing properties. The construction method comprises the following steps: pretreating ardealite slag to obtain treated alpha-semi-hydrated ardealite, compounding pretreated F-grade low-calcium fly ash, carrying out alkali excitation by using NaOH, adding 1-2% of a biomass material, and finally carrying out maintenance. Wherein the biomass material is formed by compounding modified cassava starch and raffinose according to a certain proportion. The phosphogypsum-based composite geopolymer prepared by the method can be used for road base materials, building wall materials, thermal insulation materials and the like. Compared with the prior art, the polysaccharide biomass material added in the invention can not only prolong the geopolymer coagulation time, but also shorten the final setting time and reduce the maintenance cost, and can also inhibit gel pores and microcracks caused by no continuous output of ettringite in large-dosage phosphogypsum; the later strength of the ardealite-based geopolymer is improved; the construction performance of the ardealite-based geopolymer The green biomass material cassava starch and raffinose are adopted as additives, resource utilization of the ardealite slag is achieved, low carbon and environmental protection are achieved, and the preparation method is simple, low in cost and wide in application prospect.

The application provides an NKT cell preparation and a preparation method and application thereof, and belongs to the field of biological medicine. The NKT cell preparation provided by the application comprises NKT cells, a compound electrolyte injection, 5%-10% human blood albumin, 5%-10% raffinose and 2%-5% lysine, wherein the concentration of the NKT cells in the cell preparation is 1.0x10 6 -1.0x10 8 The prepared finished preparation has a long shelf life and can be stored stably at 4 DEG C for more than 144 hours and stored stably at-80 DEG C for at least 12 months.

The invention relates to the technical field of molecular diagnosis, in particular to a multiple reverse transcription polymerase chain reaction detection reagent buffer solution and a kit. According to the invention, multi-factor comprehensive research is carried out on multiple reverse transcription polymerase chain reactions, especially on the performance of a one-step RT-PCR reaction system constructed by taking Z05 enzyme as a bifunctional enzyme raw material, so that the influence of a polyhydroxy compound as an additive on various performance indexes of the reaction system is fully analyzed; a specific polyhydroxy compound (Ficoll 400) is creatively selected to be compounded with pinotriose to serve as a reaction buffer reagent additive, a synergistic interaction effect is generated, and especially under a buffer reagent system preferably constructed by a Tricine buffer solution, the final fluorescence value of an RT-PCR fluorescent quantitative reaction system is more efficiently increased, the signal-to-noise ratio is increased, and the detection accuracy is improved.

The invention relates to a specific chromatogram construction method for distinguishing iron stick yam and counterfeit products thereof based on HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) and application of the specific chromatogram construction method, the iron stick yam and the counterfeit products thereof can be effectively distinguished, and the quality and the medicinal value of the iron stick yam are guaranteed. Then, detecting by using a high performance liquid chromatography electrospray detector under specific chromatographic conditions, and finally, according to a detection result, drawing a standard characteristic chromatogram of the iron stick yam medicinal material as well as the counterfeit product and the reference medicinal material of the iron stick yam medicinal material; the specific chromatogram is applied to quality detection, evaluation and control of the Tiegun yam medicinal material, or is applied to distinguishing the Tiegun yam and counterfeit products of the Tiegun yam. According to the specific chromatogram construction method, the iron stick yam and the counterfeit potatoes, cassava, sweet potatoes and dioscorea nipponica can be identified according to whether the raffinose exists in the chromatographic peak or not, the product quality and medicinal value of the iron stick yam are effectively guaranteed, and the specific chromatogram construction method has practical application value.

The invention discloses a method for synchronously detecting five functional components in a beet product, and belongs to the technical field of analytical chemistry. The method comprises the following steps: diluting a beet product with water, fixing the volume, and filtering to prepare a sample solution; injecting the sample solution into a high performance liquid chromatography system, and carrying out gradient elution separation by adopting a hydrophilic interaction chromatographic column; an evaporative light-scattering detector is used for synchronously detecting and quantifying betaine, cane sugar, fructose, glucose and raffinose. According to the method, synchronous detection of five components is realized for the first time, and the problems that a traditional method needs to be detected for several times and the efficiency is low are solved. The method shows excellent precision (RSD < 0.2%) and anti-interference capability in a complex matrix, and has good universality and stability. In addition, the method also reveals the fundamental difference between the molasses and the massecuite in component, and provides a key technical basis for raw material selection for betaine extraction.