50 results about "Protein solubility" patented technology

The invention discloses an artificial sensor element combination, nucleic acid for coding the artificial sensor element combination, a recombinant expression vector containing the nucleic acid, a host cell and application of the artificial sensor element combination. The artificial sensor element combination comprises a polypeptide chain 1, a polypeptide chain 2 and a specific substrate, and the method is used for high-throughput detection of protein solubility, stability and enzyme activity. When the artificial sensor element combination is used for detecting protein properties, the problems of inaccuracy, false positive and the like of soluble detection of a conventional protein soluble sensor caused by dynamic characteristics of protein expression, folding and aggregation processes can be at least solved.

The invention discloses a method for predicting protein solubility, a neural network training method, a neural network training device, equipment and a medium, and relates to the technical field of biological information, in particular to the technical field of biological computing. The method for predicting the protein solubility comprises the following steps: acquiring a target protein sequence comprising a plurality of amino acid residues; coding the target protein sequence by using a protein pre-training model to obtain respective coding feature vectors of a plurality of amino acid residues; determining respective replacement score vectors of the plurality of amino acid residues and contact probabilities of the plurality of amino acid residue pairs; processing the respective coding feature vectors of the plurality of amino acid residues and the contact probabilities of the plurality of amino acid residue pairs by using a first graph neural network; processing the respective replacement score vectors of the plurality of amino acid residues and the contact probabilities of the plurality of amino acid residue pairs by using a second graph neural network; and inputting results processed by the two graph neural networks into a prediction sub-network to obtain a prediction result of the solubility of the target protein sequence.

The invention discloses a method for improving the heterologous soluble expression efficiency of cytochrome P450, which is characterized in that plasmids containing coding genes of fungus-derived cytochrome P450 truncated protein and plasmids containing molecular chaperones are jointly transferred into host bacteria to obtain recombinant genetically engineered bacteria for improving the heterologous soluble expression efficiency of cytochrome P450. Through host screening, molecular chaperonin and cytochrome P450 gene modification, the soluble expression efficiency of cytochrome P450 in Escherichia coli is improved, and the problems of poor protein solubility and low expression efficiency when CYP450 is expressed in Escherichia coli at present are solved.

The invention relates to a method for improving the protein solubility of walnut meal by combining ultrasonic-assisted restrictive enzymolysis with glycosylation modification, which comprises the following steps: degreasing walnut meal to obtain walnut degreased powder; the method comprises the following steps: dissolving walnut defatted powder by using deionized water, treating the walnut defatted powder by using double-frequency ultrasonic waves, magnetically stirring, centrifuging, taking supernatant, stirring, centrifuging, taking precipitate, and carrying out vacuum freeze drying to obtain walnut meal protein; carrying out restrictive enzymolysis on the walnut meal protein by using trypsin, and inactivating protease contained in enzymatic hydrolysate after enzymolysis; and adding the inactivated enzymatic hydrolysate into maltodextrin for a glycosylation reaction, after the glycosylation reaction, collecting a solution, freeze-drying to obtain a glycoprotein conjugate, and storing under a drying condition. According to the method disclosed by the invention, ultrasonic-assisted restrictive enzymolysis is combined with glycosylation modification to improve the protein solubility of the walnut meal, so that the reaction steps can be optimized, the reaction time is saved, the protein solubility is greatly improved, and the deep processing and comprehensive utilization of the walnut industry are further promoted.

The present invention relates to a method of treating a material comprising corn protein with a protein deamidase. The enzymatically treated material has improved water holding capacity, emulsifying capacity, and / or improved corn protein solubility compared to a material obtained using the same method but without the use of a protein deamidase.

The invention relates to a protein solubility prediction method and system based on GCN and an improved attention network. The method comprises the following steps: constructing a protein sequence data set; the method comprises the following steps: acquiring a protein three-dimensional structure file through AlphaFold3, and constructing a node feature vector; extracting position embedding, bidirectional projection direction features, a cross-node atom pair distance and a rotation relation, and constructing an edge feature vector; extracting global physicochemical property feature vectors of the protein; and constructing protein graph structure data, inputting the data to the graph convolutional network module and the improved graph attention network module, and performing training to obtain a graph convolutional network and an improved attention network model to perform protein solubility prediction. By extracting node and edge features and protein global physicochemical property features, subsequent model training is facilitated; and the two-way projection and the rotation relation enable the model to accurately sense the spatial orientation between residues, so that the model has more quantitative understanding on the protein folding and gathering driving force, and the accuracy of protein solubility prediction is improved.

The invention discloses a method for simultaneously measuring total acid and protein solubility of fermented soybean meal. The method comprises the following steps: S1, measuring the initial pH of a 0.2% potassium hydroxide solution; s2, weighing two fermented soybean meal samples with the same mass, respectively putting the two fermented soybean meal samples into a beaker A and a beaker B, and adding an equivalent 0.2% potassium hydroxide solution; substances in the beaker A are stirred and centrifuged, and supernate is taken to obtain to-be-detected liquid A; s3, adding a potassium hydroxide standard titration solution into the to-be-detected solution A for titration, observing the pH change of the to-be-detected solution A at any time by using an acidimeter, recording the volume of the consumed potassium hydroxide standard titration solution when the titration end point is reached, and meanwhile, performing a blank test; s4, adding a potassium hydroxide standard titration solution into the beaker B, stirring, centrifuging, taking supernate, digesting, and measuring crude protein contents W1 and W2; and S5, calculating a result. According to the method for simultaneously determining the total acid and the protein solubility of the fermented soybean meal, the accuracy of detecting the protein solubility of the fermented soybean meal is improved.

The invention discloses a construction method of a protein solubility capability prediction model, a protein solubility capability prediction method and a prediction device, and relates to the field of protein solubility capability prediction. According to the method, the accuracy, the adaptability, the practicability and the automatic integration capability of the model are remarkably improved in a protein solubility capability prediction task, multiple limitations in data quality, generalization capability, calculation cost and practical application in the prior art are overcome, and the method has good popularization prospects and industrial conversion values.

The present disclosure generally relates to compositions and methods for improving the fecal score of an animal using a new combination of probiotic Bacillus strains and administering the same to the animal. The present disclosure also relates to compositions and methods for improving gastrointestinal health in an animal using a novel combination of probiotic Bacillus strains. The present disclosure further relates to compositions and methods for increasing sugar release and protein solubility of animal feed using a combination of probiotic Bacillus strains.

The present invention provides an engineered terminal deoxynucleotidyl transferase (TdT) useful in template-independent polynucleotide synthesis, the terminal deoxynucleotidyl transferase having increased thermostability and / or increased TdT activity and / or increased protein solubility, as well as compositions comprising the said terminal deoxynucleotidyl transferase and methods comprising the use of said terminal deoxynucleotidyl transferase.

Indoleamine 2,3-dioxygenase (IDO) is a heme-binding enzyme involved in pathophysiological processes including, but not limited to, antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Aspects of the disclosure relate to variants of IDO that comprise one or more amino acid variations relative to a wild-type IDO and / or that are connected to a synthetic polymer, such as poly (ethylene glycol). Embodiments of the disclosure provide variant IDOs that have improved properties, such as resistance to oxidative conditions, protein solubility, lower propensity to aggregate in vivo, increased half-life in the circulatory system upon administration to a subject, increased binding to a cell or tissue, or any combination thereof. Accordingly, IDO variants of the disclosure can be administered to a subject in need thereof, such as a subject having, suspected of having, or at risk of developing a disease, disorder, or condition, such as one associated with inflammation.

The invention belongs to the technical field of animal nutrition, and particularly relates to a biological protein and a preparation method and application thereof. The biological protein is mainly prepared from the following raw materials in percentage by weight through fermentation: 83 to 87 weight percent of corn protein, 6.5 to 7.5 weight percent of nostoc sphaeroides, 4 to 8 weight percent of sea coconut and 2.5 to 3.5 weight percent of glucose and a microbial agent; the microbial agent is lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis; in the biological protein, the content of crude protein is 49 to 54 weight percent, the content of true protein is 42 to 48 weight percent, the content of crude fiber is 4.5 to 5.6 weight percent, and the content of total acid is 8.2 to 8.9 weight percent; the viable count is 4.5-5.5 * 10 < 9 > cfu / mL, and the protein solubility is 73-79 wt%. The biological protein produced by the invention has higher protein solubility and beneficial viable bacteria, and these properties have important meanings for improving nutrient absorption and health conditions of animals such as livestock (such as dairy cows), poultry and the like, and also can improve the laying rate of the poultry and the milk production rate of the livestock and dairy cows, so that the production cost is reduced, and the economic benefit is increased. The safety and the health degree of the food are improved, and the method has a relatively good commercial application prospect.

This invention provides material compositions and / or methods suitable for the prevention and / or treatment of protein depletion (protein deficiency), comprising material compositions that preserve the natural function of peptides / proteins while limiting and / or preventing the formation and / or aggregation of said peptides / proteins into amyloid protein. It also provides material compositions and formulations for enhancing peptide / protein solubility, stability, cycle time, receptor interaction, brain permeability, CSF half-life, and promoting peptide / protein synthesis and purification.

The invention belongs to the technical field of biological detection, and particularly relates to a porcine epidemic diarrhea recombinant rS1 antigen as well as a preparation method and application thereof. A coding sequence of the recombinant rS1 antigen sequentially comprises the following elements from an N terminal to a C terminal: a signal peptide, PEDV S1 protein and a trimer tag, and the elements are connected with one another through linkers. According to the recombinant rS1 antigen, a signal peptide capable of assisting S1 protein secretion is added to the upstream of an S gene to assist soluble expression of S1 protein in periplasmic space, so that the protein solubility is enhanced; a trimer tag GCN4 is added to the downstream to assist correct folding of S1 in the expression process, so that the protein solubility is further enhanced, and correct expression of conformation is realized.

The invention discloses a preparation method of ultrasonic-heat treatment synergistically modified flaxseed protein and application of the flaxseed protein in ice cream. The method comprises the following steps: (1) degumming pretreatment of flaxseed cake meal; (2) flaxseed protein extraction; and (3) ultrasonic-heat treatment synergistic modification. The synergistic effect of ultrasonic and heat treatment can destroy the compact structure of flaxseed protein and expose more hydrophilic groups and hydrophobic groups, so that the solubility of the protein is improved to 80% or above, the emulsifying activity index is improved by 40% or above compared with that of natural protein, and the emulsion is still stable after being stored at 4 DEG C for 30 days; when the modified flaxseed protein is applied to the ice cream, the expansion rate of the product can be increased by 15-20%, the melting rate can be reduced by 25% or above, the hardness is moderate, the taste is smooth, and meanwhile the nutritional additional value of the product is increased. According to the method, resource upgrading of the flaxseed cakes is realized, low-price byproducts are converted into high-added-value food ingredients, the industrial chain of flaxseeds is prolonged, and the comprehensive benefits of the industry are improved.

In a process for producing protein preparations from sunflower seeds a protein-containing flour made of dehulled and deoiled sunflower seeds preferably having a protein solubility in water at pH 6 of >15% by mass and / or at pH 7 of >25% by mass relative to the protein content in the flour is subjected to at least one extraction step with water at a pH of more than 4 and less than 9 to obtain a liquid phase as an extract and a solids-rich phase as a raffinate. Before and / or during the extraction step the oxygen concentration in the water is reduced to a value <7 mg / l and / or an oxidation activity is reduced by addition of antioxidant components to the water.After separation of the extract and the raffinate the extract is concentrated and / or dried to obtain a protein preparation having a high protein content. The process makes it possible to obtain qualitatively high-grade and sensorily appealing light-coloured protein preparations having a protein content greater than 75% by mass.

The application belongs to the field of biological medicine, and particularly relates to a catalytic antibody mutant for degrading Helicobacter pylori urease, and a preparation method and application thereof. The application takes wild-type catalytic antibody Ua15-L (WT) for degrading Helicobacter pylori urease as a starting antibody, rationally designs a catalytic antibody mutant for degrading Helicobacter pylori urease with better performance, and verifies through experiments that the I48C-G64C mutant is mutated at the 48th and 64th amino acids, and is mutated from isoleucine and glycine to cysteine, so that a disulfide bond is formed between the two, which is helpful to improve the thermal stability of the catalytic antibody. On this basis, the mutations L9E, L54R and T14Q-I15P are introduced to increase the protein solubility and expression amount. Compared with the wild-type catalytic antibody for degrading Helicobacter pylori urease, the mutant 1 (L9E-I48C-G64C) increases the Tm value by 6 DEG C, increases the solubility by 81%, and increases the expression amount by 4 times; and the time for the urease fragment to be hydrolyzed by half at 37 DEG C is shortened by 52% compared with the wild type.

The invention belongs to the field of biological medicine, and particularly relates to a catalytic antibody mutant for degrading helicobacter pylori urease as well as a preparation method and application of the catalytic antibody mutant. A wild type helicobacter pylori urease degrading catalytic antibody Ua15-L (WT) is used as a starting antibody, a higher-performance helicobacter pylori urease degrading catalytic antibody mutant is obtained through rational design, experiments prove that the 48th amino acid and the 64th amino acid of the I48C-G64C mutant are mutated, isoleucine and glycine are mutated into cysteine respectively, and the mutant is converted into cysteine. A disulfide bond is formed between the two, so that the thermal stability of the catalytic antibody is improved. On the basis, mutations of L9E, L54R and T14Q-I15P are introduced, so that the solubility and the expression quantity of the protein are increased. Compared with a wild type catalytic antibody for degrading helicobacter pylori urease, the mutant 1 (L9E-I48C-G64C) has the advantages that the Tm value is increased by 6 DEG C, the solubility is increased by 81%, and the expression quantity is increased by 4 times; and the time for hydrolyzing half of the urease fragment at 37 DEG C is shortened by 52% compared with that of the wild type.

The present invention discloses a method for improving the structure of eel myofibrillar protein, which belongs to the field of food processing. The present invention extracts eel myofibrillar protein, adds nutmeg and rosemary effective ingredient extracts, and synergistically prepares a protein conjugate, effectively improves the protein structure, regulates the protein aggregation state, and significantly improves the solubility, antioxidant properties and in vitro digestibility of eel myofibrillar protein, providing an optimization direction for existing eel seasonings. The present invention uses spice extracts to prepare protein solubility, antioxidant properties, and digestibility that are higher than the coupling product of gallic acid and protein at an appropriate concentration. While improving the structure of eel myofibrillar protein and enhancing antioxidant properties, it increases the flavor characteristics of eel, providing a low-sugar and low-salt eel product with a unique flavor. The present invention has low energy consumption, no pollution, high safety, low cost, and various parameters are easy to control, the process is simple, and it has good promotion and application prospects in industrial production practice.

The invention relates to a method of treating a material comprising corn protein with a protein deamidase. The enzyme treated material has improved water holding capacity, emulsifying capacity and / or improved corn protein solubility compared to a material obtained using the same method but without the protein deamidase.

The invention relates to the technical field of agricultural product processing, in particular to a method for establishing and evaluating a prediction model suitable for processing dissolved leguminous plant varieties. According to the model building method, the comprehensive value Y suitable for processing the soluble leguminous plant variety is obtained, and a plurality of machine learning algorithms are adopted for comparison on the basis of sensory quality related indexes, physicochemical nutritional quality related indexes and the like, so that the optimal leguminous plant protein solubility quality evaluation and prediction model is built, the model accuracy is high, and the prediction effect is good. According to the evaluation method, the leguminous plant varieties suitable for processing the dissolution type can be rapidly screened, the evaluation process is greatly simplified, researchers and enterprises can rapidly judge whether the leguminous plant varieties are suitable for development of the dissolution type products or not, and the requirement of the market for high-quality dissolution type leguminous plant protein products can be met.

The invention discloses a cottonseed protein solubility improving method based on pH shift synergistic homologous raffinose compounding, and relates to the technical field of plant protein modification. The method for improving the solubility of the cottonseed protein comprises the following steps: uniformly mixing raffinose, the cottonseed protein and water, carrying out alkaline pH (Potential of Hydrogen) migration treatment, centrifuging to obtain supernate, and dialyzing the supernate to obtain a cottonseed protein-raffinose compound. According to the method, an alkaline pH shift-raffinose in-situ compounding synergistic strategy is put forward for the first time, the low molecular weight characteristic of the cottonseed byproduct raffinose is utilized, the raffinose is accurately embedded into a cottonseed protein hydrophobic structural domain, reaggregation of the protein is blocked through the dual effects of hydrogen bond combination and electrostatic repulsion, and breakthrough improvement of the solubility of the cottonseed protein is achieved.

This application relates to the field of antibody technology, specifically providing a truncated fragment of the African swine fever virus pB602L protein, an antibody thereof, or an antigen-binding fragment thereof, and their applications. The truncated fragment, encoded by SEQ ID NO.1, eliminates mismatched disulfide bonds and repetitive sequences by deleting the first 200 amino acids from the N-terminus, thereby improving protein solubility and immunogenicity. The antibody thereof or its antigen-binding fragment contains the heavy chain variable region shown in SEQ ID NO.2 and the light chain variable region shown in SEQ ID NO.4, exhibiting a blocking rate of greater than 90% against the pB602L protein. This truncated fragment and antibody demonstrate high specificity and sensitivity, and can be used for the detection, diagnosis, and evaluation of vaccine immunization efficacy for African swine fever virus, providing an important tool for the prevention and control of African swine fever.

The invention relates to a protein solubility prediction method and device based on deep learning, computer equipment and a storage medium, and the method comprises the steps: obtaining a to-be-predicted sample which is a protein amino acid sequence; according to an ESM-2 model and the to-be-predicted sample, a high-dimensional feature vector is obtained, and each feature dimension of the high-dimensional feature vector corresponds to an abstract representation component of a protein sequence in a semantic space of the ESM-2 model; obtaining a protein solubility prediction probability according to the high-dimensional feature vector and a depth residual multi-layer perceptron model; and obtaining a protein solubility prediction result according to the protein solubility prediction probability. The accuracy of protein solubility prediction can be improved.

The embodiment of the invention discloses medicago sativa functional protein powder and a preparation method thereof. The protein content of the alfalfa functional protein powder is greater than or equal to 85%, the protein solubility is 92-95%, the emulsifying activity is greater than or equal to 70%, and the foamability is greater than or equal to 450%; the activity of superoxide dismutase (SOD) is larger than or equal to 250 U / g, the polysaccharide content of the ganoderma lucidum is larger than or equal to 2.0 mg / g, and the flavone retention rate is larger than or equal to 90%. According to the preparation method, a brand new fermentation system is constructed by introducing artificial ganoderma lucidum life active bacteria, and ultrasonic-enzymolysis synergistic extraction and low-temperature freeze-drying coupling drying processes are combined, so that the alfalfa functional protein powder with high protein content, high-activity metabolites and high functional characteristics is prepared; the problems that existing alfalfa protein powder is low in extraction rate, poor in activity and free of unique functions are solved.

The invention belongs to the technical field of biological information, and relates to a method and device for predicting the influence of amino acid variation on protein solubility and a medium. Inputting the characteristic values in the characteristic value set into a three-classification module, and outputting first probability vectors that the variant amino acids belong to different prediction categories; inputting feature values in the feature value set into three two-layer three-classification modules corresponding to different prediction categories, and based on the output of each two-layer three-classification module, obtaining a second probability vector, a third probability vector and a fourth probability vector of the variant amino acid belonging to different prediction categories; and inputting the first probability vector, the second probability vector, the third probability vector and the fourth probability vector into a meta-learner, and outputting a prediction category of the variant amino acid. According to the scheme, the prediction model constructed based on the multi-layer stacking strategy covers all dichotomous and trichotomous possibilities, and high prediction precision and high prediction efficiency are achieved.

The invention belongs to the technical field of agricultural product storage, and particularly relates to a preparation method of egg white liquid with high freeze-thaw stability. According to the invention, the oligosaccharide cryoprotectant with excellent cryoprotection capability is used for inhibiting ice crystal growth and recrystallization in the egg white freezing and thawing process and reducing the content of freezable water, so that the egg white liquid with high freezing and thawing stability is obtained. The preparation method comprises the following specific steps: beating and separating eggs, and then collecting fresh egg white liquid; homogenizing and filtering egg white liquid; adding an oligosaccharide cryoprotectant into the egg white liquid and carrying out secondary homogenization; packaging to obtain a finished product. Compared with untreated fresh egg white liquid, the finally prepared high-freeze-thaw-stability egg white liquid added with trehalose and soybean oligosaccharides has the advantages that the freezable water content is remarkably reduced, the system can be kept uniform without phase separation after five times of freeze-thaw cycles, the protein solubility is improved, the processing characteristic is remarkably improved, the gel strength is respectively improved from 259.33 g to 433.33 g and 371.17 g, and the freeze-thaw stability is improved. And the water binding capacity is respectively increased to 49.82% and 44.74% from 36.23%.

The invention discloses protein glutaminase as well as a production strain and application thereof. The preservation number of the Chryseobacterium lactis FET3 is CGMCC (China General Microbiological Culture Collection Center) NO: 33780, and the amino acid sequence of the produced protein glutaminase is as shown in SEQ ID NO. 4. The activity of the PG-producing enzyme of the Chryseobacterium lactis FET3 reaches 0.319 U / mL, the PG-producing enzyme shows unique catalytic advantages on casein and soybean protein substrates, and the solubility, foamability and emulsibility of protein can be remarkably improved; meanwhile, a double-peptone composite fermentation culture medium is innovatively developed, so that the enzyme production capacity of the FET3 strain is improved to 1.52 U / mL; in addition, the PG is purified by combining gradient low-temperature ethanol precipitation with an anion exchange chromatography technology, and finally the high-purity CL-PG enzyme is obtained. The invention provides an innovative technical scheme for efficient PG enzyme production and protein function improvement, and has important industrial application value.

Material compositions and / or methods useful for the prophylaxis and / or treatment of a-syn protein depletion (proteinopenia) are provided, including material compositions that retain native function of a-syn protein while limiting and / or preventing amyloid formation and / or aggregation of said protein. Material compositions and formulations for enhancing protein solubility, stability, circulation time, receptor interaction, brain penetrance, CSF halflife, and for facilitating peptide / protein synthesis and purification are also provided.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.