140 results about "Expression Library" patented technology

The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.

The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes.

The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.

The invention discloses an expression sending method and an expression sending device, and belongs to the field of an internet technology. The method comprises the steps of acquiring a face image of a user when communication information is sent and received; generating a user expression of an existing mood of the user corresponding to the face image, wherein the user expression is at least one of a picture expression and a character expression; and transmitting the user expression to an opposite terminal in the process of sending and receiving communication information. According to the expression sending method provided by the invention, the problems that expression pictures in an expression library provided by a communication client cannot express the expression state of the user cannot be accurately expressed can be solved; furthermore, an effect that the communication information sent and received by the user takes the user expression of the existing mood of the user so as to accurately express the existing expression state of the user can be achieved.

An expression library comprising a repertoire of nucleic acid sequences cloned from a non-immunized source, each nucleic acid sequence encoding at least part of a variable domain of a heavy chain derived from an immunoglobulin naturally devoid of light chains (VHH) wherein the extent of sequence variability in the library is enhanced compared to the corresponding naive expression library by introducing mutations in one or more of the complementarity determining regions (CDRs) of the nucleic acid sequences or by generating alternative combinations of CDR and framework sequences not naturally present in the naive library repertoire. Also disclosed is the use of such a library in preparing antibodies, more particularly antibody fragments.

The invention discloses a voice conversion method, a voice conversion device and a mobile terminal. The method comprises the following steps: after receiving voice information, identifying the voice information; converting the voice information into corresponding text information; and then, calling a corresponding expression symbol from an expression library in accordance with the text information, converting the text information into the corresponding expression symbol and displaying the expression symbol. The invention has the beneficial effects that a user, in a process of using the mobile terminal, can directly display the received voice information in the form of the expression symbol, so that user's emotion can be intuitively expressed and the interesting of chat can be enhanced as well. Meanwhile, the user, not affected by environmental factors, can always pay attention to important information; and correspondingly, the phenomenon that the user uses a single voice chat mode for a long time can be avoided as well, so that user experience is improved to a great extent.

The invention discloses a method and a device for acquiring expression meanings, and belongs to the technical field of communications. The method comprises the following steps of: acquiring expression data in a picture to be detected, or acquiring corresponding expression data according to expression characteristics input by a user; comparing the acquired expression data with the expression data in a micro expression library, wherein the micro expression library is pre-established and contains at least two expressions, and each expression has corresponding expression data; and if the micro expression library has matched expression data, acquiring and presenting the expressions corresponding to the matched expression data and the meanings of the expressions. The device comprises an expression data acquisition module and an expression acquisition module. The acquired expression data are compared with the pre-established micro expression library to acquire the matched expression data so as to acquire the expressions and the meanings to be acquired. The method and the device have low requirements for operation personnel, are easily popularized, do not need expensive special instruments, save the cost, and are simple, practical and effective.

The present invention concerns methods and compositions for the construction of a series of stable vectors for genomic library construction useful in Gram negative species. In certain embodiments, the vectors contain the pBBR1 replicon, capable of to stable replication in a broad range of Gram negative species. In various embodiments, the plasmid vectors may also contain bidirectional, rho-independent transcriptional terminators flanking the multiple cloning site, which allows for greater insert stability, and thus, greater genomic representation. Each vector may vary in its selection marker region, mobilization function, and promoter used to express insert sequences. These vectors are of use in the screening of highly representational genomic libraries in a broad variety of Gram negative species.

The invention discloses a robot system and method for detecting a human face and recognizing emotion. The system comprises a human face expression library collecting module, an original expression library building module, a feature library rebuilding module, a field expression feature extracting module and an expression recognizing module. The human face expression library collecting module is used for collecting a large number of human face expression color image frames through a video collecting device and processing the human face expression color image frames to form a human face expression library. The original expression library building module is used for extracting expression features after removing image redundant information of training images in the human face expression library to form an original expression feature library. The feature library rebuilding module is used for rebuilding the original expression feature library as a structuralized hash table through the distance hash method. The field expression feature extracting module is used for collecting field human face expression color image frames through the video collecting device and extracting field expression features. The expression recognizing module is used for recognizing the human face expression through the k neighbor sorting algorithm in the feature library in which the field expression features extracted by field expression feature extracting module are rebuilt.

The invention discloses a facial expression recognition method, which belongs to the field of image processing. The facial expression recognition method disclosed by the invention comprises the following steps of: selecting a plurality of neutral expression images of different people and a plurality of various expression pictures of the same people with the neutral expressions from an expression library, and extracting the position information vectors of N characteristic points respectively; obtaining bottom-layer expression characteristic point information vectors feij corresponding to different expression types on the basis of the vector difference of the latter and the former, and training the SVM classifiers of the various expressions on the basis of the feij; conveying the feij each SVM classifier, and calculating the distance from the feij to the hyperplane of each SVM classifier to form an expression characteristic library; extracting the position information vectors of N characteristic points from the neutral expression images of the people to be recognized and the images input in real time and to be recognized, calculating the vector difference of the latter and the former, and conveying the vector differences in each SVM classifier to carry out characteristic conversion processing, so as to obtain a characteristic vector; taking the characteristic vector and a characteristic vector corresponding to an Euclidean distance in the expression characteristic library as the recognized expression. The facial expression recognition method disclosed by the invention is capable of improving the accuracy and real-time performance of recognition.

The invention relates to a facial expression image recognition method based on an expert system. According to the method, inference and recognition of the facial expression of a preprocessed image are carried out through the expert system established on the basis of an expression image processing method and the function of a traditional computer program. The method comprises the following steps: (1) capturing an image in a video, acquiring user information in the video, then carrying out identity verification through image processing and image characteristic extraction, acquiring characteristic parameters of the expression image of a user, determining a user expression library, and establishing the expert system for facial expression recognition; (2) carrying out imaging processing and image characteristic extraction on the image captured in the video, acquiring the characteristic parameters generated when the degree of the expression of the user is maximized, comparing the characteristic parameters with parameters, determined in the step (1), of image training samples in the user expression library, and finally outputting the statistical result of facial expression recognition through an inference engine of the expert system. Compared with the prior art, the method has the advantages of being high in recognition speed and the like.

The present invention provides a method for the screening of nucleic acid fragment expression libraries and selecting encoded peptides based upon their ability to modulate the activity of a target protein or nucleic acid and assume conserved conformations compatible with albeit not reiterative of the target protein or nucleic acid. The present invention also provides methods for the diagnosis and treatment of ischemia. The present invention also provides c-Jun dimerization inhibitory peptides and analogues thereof that are useful for treatment of ischemia.

The invention discloses a chat picture control method and device, computer equipment and a storage medium, and the method comprises the steps: obtaining chat information of a current display interface, the chat information comprising one or more of text information, picture information or voice information; identifying a first keyword of the chat information, and matching reply information and a target expression picture mapped by the reply information from a preset database; and when it is detected that an input cursor exists in the chat input box, popping up a target expression picture for auser to select so as to reply the chat information. In the whole process, the picture does not need to be manually searched in the expression library, and the picture capable of being used as the reply is automatically popped up according to content recognition, so that the message reply picture searching time is saved. Chat reply becomes more convenient and faster.

The present invention relates to methods for efficiently generating recombinant monoclonal antibodies derived from B cells of a non-human host which has been immunochallenged with one or more target antigens. The methods comprise the steps of identifying and isolating B cell that bind to the antigen by FACS, and recombining and enriching for thousands of cells to create a B cell library. Related products and methods, such as methods of producing expression libraries, are also disclosed.

Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite, pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

The present invention relates to a method for identification and isolation of binding molecules having a selective affinity for a ligand. More specifically, this invention provides a process for the ultra high throughput screening of binding molecules from expression libraries containing billions of independent clones without the biases and limitations of other high throughput screening methods such as panning. Additionally, the present invention provides a method for the production of expression libraries essentially free of clones encoding non-functional molecules.

The development of general approaches for the isolation of efficient antivirals is becoming increasingly important. The genetic suppressor element (GSE) technology is an approach based on the functional expression and selection of efficient genetic inhibitors from random fragment libraries derived from a gene or genome of interest. We have applied this technology to isolate potent genetic inhibitors against the human immunodeficiency virus type 1 (HIV-1) The strategy employed involved the following steps: 1) fragmenting the HIV-1 genome into 100-700 base pair (bp) fragments; 2) inserting the fragments into expression vectors to form an expression library; 3) transferring the expression library into a population of cells (e.g., OM10.1) containing an inducible latent HIV-1 provirus; 4) selecting a subpopulation of cells which contain a subset of the expression library enriched for HIV-1 GSE by monitoring the expression of a cellular (e.g., CD4) or viral (e.g., p24) marker associated with HIV infection; 5) recovering the GSE from the selected cell population. The GSEs identified clustered in seven narrowly defined regions of the HIV-1 genome and were found to be functionally active. These elements are potential candidates for the gene therapy of AIDS. The developed approaches can be applied to other viral pathogens, as well as, for the identification of cellular genes supporting the HIV-1 life cycle.

The present invention relates to a novel method for the identification and/or characterization of clones conferring a desired biological property from an expression library. The method of the invention comprises the step of analyzing for the expression of at least one (poly)peptide, such as a tag expressed as a fusion protein, together with a recombinant insert of a clone of said expression library, wherein the clones of said expression library are arranged in arrayed form. Said (poly)peptide may be fused N-terminally or C-terminally to said insert. The method of the invention further comprises the steps of contacting a ligand specifically interacting with a (poly)peptide expressed by the insert of a clone conferring said desired biological property with a first replica of said library of clones in arrayed form and analyzing said library of clones for the occurrence of an interaction, and/or carrying out a hybridization or an oligonucleotide fingerprint with a nucleic acid probe specific for the insert of a clone conferring said desired biological property with a second replica of said library of clones arranged in arrayed form and analyzing said library of clones for the occurrence of a specific hybridization. Finally, the method of the invention requires the identification of clones wherein an expression of the at least one (poly)peptide in step (a) and/or an interaction in step (b) and/or a hybridization or an oligonucleotide fingerprint in step (c) can be detected. The present invention also relates to a kit useful for carrying out the method of the invention.

The invention is suitable for the technical field of instant messaging and provides a pushing method and a pushing device of a session expression, a terminal device and a computer readable storage medium. The pushing method comprises the steps of obtaining electrodermal response data of a user of a current intelligent terminal in a session interface of the intelligent terminal; determining the current emotion type of the user based on the obtained electrodermal response data; and pushing a session expression corresponding to the determined emotion type in the session interface based on the determined emotion type so as to enable the user to select the pushed session expression as the session content. According to the pushing method of the session expression, the user is prevented from inputting characters or selecting the session expression from a large number of session expression libraries, and the convenience of using the session expression for instant messaging by the user is improved.