11 results about "Provirus" patented technology

The invention discloses chicken infectious anemia virus-like particles as well as a preparation method and application thereof. The invention discloses a virus-like particle vaccine for preventing chicken infectious anemia. The virus-like particle vaccine comprises VP1 and VP2 proteins of chicken infectious anemia viruses. Chicken infectious anemia VP1 and VP2 proteins are expressed by using a baculovirus expression system, immunoblotting shows that the two proteins are successfully expressed in sf9 cells, electron microscope observation finds that the expressed proteins can be autonomously assembled into complete chicken infectious anemia virus-like particles, and the chicken infectious anemia virus-like particles have a space structure similar to that of an original virus and can be used for preparing chicken infectious anemia virus-like particles. Meanwhile, the virus-like particles have the advantages of high titer, high safety, capability of stimulating humoral immunity and cellular immunity and the like. The preparation method disclosed by the invention is simple, can be used for preparing the antigen protein of the chicken infectious anemia virus on a large scale, is high in expression quantity and short in time consumption, greatly reduces the production cost, and is suitable for large-scale production.

The application discloses a benzotriazole compound, a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The compound has structures as shown in general formula (I), (II) and (III). A fragment containing an amide bond substituted amino acid ester or amino acid structure is introduced into the system based on benzotriazole to synthesize a series of benzotriazole compounds containing the amino acid ester or amino acid structure. The compound has a good inhibiting effect on plant pathogenic viruses, and especially has a good inhibiting effect on plant pathogenic viruses such as tobacco mosaic virus.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses a kit for rapidly and quantitatively detecting an integrated HIV (Human Immunodeficiency Virus) previrus genome and application of the kit. The kit comprises an anchor primer in an Alu region in an HIV virus, and degenerate primers in gag, nef and LTR regions of an HIV sequence. According to the kit disclosed by the invention, after pre-amplification, the integrated copy number AU-LTR detected by the QPCR is obviously improved compared with AU-gag and AU-nef, and the capture efficiency is higher. Meanwhile, the kit provided by the invention can be used for carrying out three-generation sequencing on a detected sample with a relatively high integration level on the basis of Alu-LTR capture and detection of an integrated copy number, and can be used for carrying out sequence composition and genome position analysis on a storage library of HIV (Human Immunodeficiency Virus).

The present invention relates to a nucleic acid encoding a recombinase capable of recombining asymmetric target sequences of SEQ ID NO:1 within the long terminal repeat of proviral DNA of a plurality of HTLV-1 strains, the recombinase having specific amino acid exchanges compared to the sequence of cre recombinase. The invention also provides expression vectors comprising said nucleic acids and cells comprising the same, the recombinase in protein form and pharmaceutical compositions comprising them. A method for provision of such recombinases is also provided. The invention also provides a method of treating a HTLV-1-infected subject, wherein the subject optionally has adult T-cell leukemia (ATL) and / or HTLV-1-associated myelopathy (HAM).

The present invention relates to methods for preparing expression vectors encoding well-tolerated and highly specific tailored recombinases that are capable of recombining asymmetric target sequences within the long terminal repeats (LTRs) of proviral DNA of a variety of retroviral strains that can be inserted into the genome of a host cell, as well as the expression vectors obtained, the cells transfected with these, the recombinases expressed and pharmaceutical compositions comprising said expression vectors, cells and / or recombinases. The pharmaceutical compositions can be used, for example, for the treatment and / or prophylaxis of retroviral infections, in particular HIV infections. In particular, the present invention relates to well-tolerated and highly specific tailored recombinases that are capable of combining asymmetric target sequences in more than 90% of HIV-1 strains, thereby excising HIV-1 sequences and expression vectors encoding them.

The invention relates to a watermelon breeding method for improving disease resistance, which comprises the following steps: S1, establishing a plurality of groups of parallel evolution systems, each group comprising a donor region and a verification region; s2, inoculating the ith virus to the ith group of donor region plants (i = 1, 2... N); s3, after inoculation, an in-vitro leaf identification method is adopted to eliminate plants; s4, extracting dead plant viruses; s5, respectively inoculating the extracted strain and the original strain to the plants in the verification area, if the evolutionary virus disease is more serious, replacing the original virus with the evolutionary virus, inoculating the evolutionary virus to the donor area, and skipping to execute S3; s6, selfing the same group of plants to cultivate seedlings; and S7, hybridizing the watermelon plants in different groups in pairs, planting watermelon seedlings cultivated by hybridizing the two groups in a new donor area and a verification area, inoculating watermelon viruses inoculated by donors of previous generations to the watermelon plants, and skipping to execute the step S3 until the last group is left. Watermelon seedlings with stable antibodies of various protoviruses and evolutionary viruses can be cultivated in a short time.

The utility model relates to the technical field of accessories of virus filtering equipment, in particular to a filter element for removing original viruses. According to the filter element for removing the original viruses, provided by the utility model, the technical problems that a filter element in the prior art is poor in filtering effect and a filtering part cannot be independently replaced are solved. The utility model discloses a filter element for removing original viruses. The filter element comprises a shell assembly, the filter element assembly is arranged in the shell assembly; through the arrangement of the upper filter element, the antibacterial and disinfection filter element and the lower filter element, harmful substances in gas can be effectively filtered in the process that the gas passes through the upper filter element, the antibacterial and disinfection filter element and the lower filter element, particularly, the filter element assembly can filter original viruses through the arrangement of the antibacterial and disinfection filter element, and the filtering effect of the filter element is effectively optimized. The shell comprises the upper shell body and the lower shell body, so that the shell assembly can be conveniently disassembled, and the upper filter element, the antibacterial sterilization filter element and the lower filter element can be conveniently and independently replaced.

The application discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses and belongs to the technical field of pesticide antiviral agents.Taking a tobacco mosaic virus coat protein as an example, small molecules including sugar rings, such as Swertiamarin I (A0) and ZINC85593196 (B0), are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. Based on this, further optimization is carried out, and six compounds similar to the structure of A0, such as 4-methylumbelliferone-beta-D-xyloside (A1), gardenoside (A2), 4-methylumbelliferone-beta-D-pyranoside (A3), 4-methylumbelliferone-beta-D-pyranoglucoside (A4), catalpol (A5) and esculin (hemihydrate) (A6), are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active ingredients screened by the above method have good inhibitory effect on plant viruses.

In some aspects, the invention relates to methods for determining the proportion of intact, hypermutated, deleted, and / or defective proviruses in a sample of nucleic acids, e.g., a sample obtained from a subject. The subject may be a human subject. The virus may be HIV.