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Novel coronavirus vaccine as well as preparation method and application thereof

A coronavirus and vaccine technology, applied in the field of novel coronavirus vaccine and its preparation, can solve the problems of low production efficiency and low immunogenicity, and achieve the effects of improving production effect, enhancing immune efficacy, and good biological safety

Pending Publication Date: 2020-11-13
李伟宏 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantage of traditional inactivated vaccines is that it takes several months to cultivate the antigenic microorganisms, and the production efficiency is low; in addition, the immunogenicity of inactivated vaccines is lower than that of attenuated vaccines, and multiple vaccinations are required to activate sufficient immune responses
The disadvantage of live attenuated vaccines is the risk of other diseases caused by mutations returning to the ancestors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] This embodiment provides a novel coronavirus 2019-nCoV vaccine, the preparation method of which is as follows:

[0061] (1) Inoculate, cultivate and purify the novel coronavirus:

[0062] (1.1) Inoculation: Inoculate the new coronavirus into chicken embryos hatched for 10 days;

[0063] (1.2) Cultivation: In a 10,000-liter culture tank, put clear water, virus strains, and nutrient granules, keep the room temperature stable, stir evenly, and collect the virus after 2 months;

[0064] (1.3) Purification: Ultrafine filtration membrane is used for purification treatment.

[0065] (2) The purified novel coronavirus is mixed with water to prepare a novel coronavirus aqueous solution with a concentration of 0.5 mg / mL, and then mixed with chitosan hydrochloride (number-average molecular weight of 20 kDa, The deacetylation degree is 87%) aqueous solution is mixed at 20 DEG C for 10 min, and then mixed with a concentration of 1.5 mg / mL sodium tripolyphosphate aqueous solution a...

Embodiment 2

[0067] This embodiment provides a novel coronavirus 2019-nCoV vaccine, the preparation method of which is as follows:

[0068] (1) Inoculate, cultivate and purify the novel coronavirus:

[0069] (1.1) Inoculation: Inoculate the new coronavirus into chicken embryos hatched for 10 days;

[0070] (1.2) Cultivation: In a 10,000-liter culture tank, put clear water, virus strains, and nutrient granules, keep the room temperature stable, stir evenly, and collect the virus after 2 months;

[0071] (1.3) Purification: Ultrafine filtration membrane is used for purification treatment.

[0072] (2) The purified novel coronavirus is mixed with water to prepare a novel coronavirus aqueous solution with a concentration of 0.1 mg / mL, and then mixed with chitosan lactate (number-average molecular weight of 50 kDa, The deacetylation degree is 89%) aqueous solution is mixed at 15 ℃ for 10 min, then mixed with the sodium tripolyphosphate aqueous solution with concentration of 1.0 mg / mL at 15 ℃ ...

Embodiment 3

[0074] This embodiment provides a novel coronavirus 2019-nCoV vaccine, the preparation method of which is as follows:

[0075] (1) Inoculate, cultivate and purify the novel coronavirus:

[0076] (1.1) Inoculation: Inoculate the new coronavirus into chicken embryos hatched for 10 days;

[0077] (1.2) Cultivation: In a 10,000-liter culture tank, put clear water, virus strains, and nutrient granules, keep the room temperature stable, stir evenly, and collect the virus after 2 months;

[0078] (1.3) Purification: Ultrafine filtration membrane is used for purification treatment.

[0079] (2) Mix the purified new coronavirus with water to prepare a new coronavirus aqueous solution with a concentration of 1.0 mg / mL, and then mix it with N-(2-hydroxypropyl)-3-tris-2 with a concentration of 2.0 mg / mL Methyl chitosan ammonium chloride (number average molecular weight is 80kDa, deacetylation degree is 92%) aqueous solution is mixed at 30°C for 5min, and then mixed with sodium tripolyph...

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Abstract

The invention relates to a coronavirus vaccine as well as a preparation method and an application thereof. The coronavirus vaccine comprises a nano-carrier and coronavirus coated in the nano-carrier,wherein the nano-carrier comprises chitosan and / or a chitosan derivative. The preparation method of the coronavirus vaccine comprises the following steps: mixing coronavirus with the carrier, and thenmixing with weak acid to form nanoparticles, namely the coronavirus vaccine. According to the invention, the coronaantigen virus is creatively coated in chitosan and / or the chitosan derivative to finish a sterilization procedure in a physical mode, so that compared with a traditional chemical sterilization mode, the production effect of the vaccine is improved, the production cost is saved, and the risk of other diseases caused by mutation and reversion of the coronaantigen virus is reduced; in addition, due to the biocompatibility and biodegradability of the chitosan or the derivative thereof, the vaccine has very good biological safety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a coronavirus vaccine and its preparation method and application, in particular to a novel coronavirus vaccine and its preparation method and application, especially to a vaccine with high safety, remarkable curative effect and simple preparation process. Novel coronavirus vaccine and its preparation method and application. Background technique [0002] Coronaviruses belong to the order Nidovirales, Coronaviridae, and the genus Coronavirus. They are important pathogens of many domestic animals and pets, including human diseases, and cause a variety of acute and chronic diseases. The Coronaviridae family can be divided into three genera, α, β, and γ. The novel coronavirus belongs to the Sarbecovirus subgenus of betacoronaviruses. [0003] Novel coronavirus pneumonia is a new infectious disease, and the population has no immunity and is generally susceptible. Novel coronavirus-infecte...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K9/50A61K39/39A61P31/14B82Y5/00
CPCA61K39/12A61K39/39A61P31/14B82Y5/00A61K9/5036A61K9/0043A61K2039/5252A61K2039/55583A61K2039/543A61K2039/57C12N2770/20034
Inventor 李伟宏
Owner 李伟宏
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