316 results about "Nano carriers" patented technology

The invention relates to the technical field of cosmetics, in particular to an acne-removing composition, lipidosome and a preparation method and application thereof. The acne-removing composition disclosed by the invention is prepared from the following components in parts by weight: 2 to 8 parts of nicotinamide, 1 to 5 parts of cortex magnoliae officinalis extract, 0.15 to 2.01 parts of glycyrrhizic acid, 0.1 to 2 parts of totarol and 0.01 to 0.15 part of hyssopus officinalis extract. The nano-carrier is adopted to wrap and deliver the acne removal composition, so that the solubility of the functional components is increased, the bioavailability of the functional components is improved, meanwhile, the irritation of the functional components is also improved, multi-target co-delivery and multi-mechanism acne removal are realized, and the acne removal composition has a remarkable acne removal effect.

The invention discloses a preparation method and application of silymarin spray curing nano-carrier powder, and relates to the technical field of medicines and the field of foods. The method comprises the following steps: firstly, constructing a silymarin-carrier compound through a molecular self-assembly technology, and then, carrying out curing treatment on a nano-carrier by adopting a spray drying technology, so as to obtain nano-powder with good flowability. According to the preparation method disclosed by the invention, the silymarin spray curing nano-carrier powder with a core-shell structure is successfully prepared by optimizing a carrier material ratio and spray drying conditions. Experimental results show that the spray-cured nano-carrier powder prepared by the method significantly improves the solubility and stability of silymarin, and enhances the bioavailability and efficacy, so that silymarin can be more efficiently absorbed and released in a targeted manner in vivo, thereby significantly improving the therapeutic effect.

The invention discloses a PEI and targeting peptide modified magnetic nano-carrier and a preparation method and application thereof. The nano-carrier is regular in morphology and uniform in particle size and has good crystallinity and magnetic performance. E5 polypeptide and RGD cyclic peptide on the surface of the nano-carrier are respectively combined with CXCR4 protein and integrin on the surface of a tumor cell, and the nano-carrier and CXCR4 are internalized into lysosome through an integrin-mediated way, so that the target protein CXCR4 is degraded. The PEI in the nano-carrier can load and deliver siRNA into tumor cells to silence the expression of CXCR4 mRNA (messenger Ribonucleic Acid). Through nucleic acid delivery and a protein targeted degradation strategy, the CXCR4 level of tumor cells is jointly reduced, the migration ability of the tumor cells is inhibited, and meanwhile, the anti-tumor effect of M1 type polarization of macrophages is activated.

The invention relates to the technical field of pollution detection, in particular to a pollution assessment method, system, equipment and medium for a refuse landfill, and the method comprises the following steps: firstly, obtaining full-spectrum induced polarization data of the landfill to construct a stratum electrochemical attribute model; resolving a retardation factor distribution field by using the physical and chemical parameters of the pollution carrier and the model; constructing a Darcy velocity field in combination with hydrogeological parameters, and generating a pollution carrier migration velocity field through retardation factor correction; and finally, streamline tracing simulation is executed based on the velocity field, so that a target monitoring well coordinate and a risk early warning level are output. The technical problems that the dominant flow channel of the structural fracture is difficult to identify under the complex geology and evaluation distortion is caused by non-synchronous migration caused by the interface electrochemical action of the micro-nano carrier in the traditional method are solved; accurate positioning of the hidden leakage channel and accurate prediction of the penetration time are achieved, and the early warning capacity and the management and control efficiency of landfill environment risks are remarkably improved.

The invention discloses an engineered bionic nucleic acid nano-vesicle as well as a preparation method and application thereof, relates to the technical field of nano biomedicine, and aims at solving the problems that in-vivo targeting efficiency and immunogenicity of a traditional cationic lipid nano-carrier are limited due to deletion and cleavage obstacles of GSDMD expression in tumor cells. The technical key point of the invention is as follows: the engineered bionic nucleic acid nano-vesicle is provided and is prepared by wrapping a cationic lipid nucleic acid drug with an exosome derived from engineered macrophages; wherein the exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1, which is as shown in SEQ. ID. NO.1. The exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1; the lipid nucleic acid medicine is prepared by loading GSDMD-N mRNA (messenger Ribonucleic Acid) shown on the basis of SEQ.ID.NO.2 on a cationic liposome. The engineered bionic nucleic acid nano-vesicle is used for preparing an oral squamous cell carcinoma diagnostic kit and a therapeutic drug.

The invention belongs to the technical field of biological medicines, and particularly relates to an iron-based nano material, a preparation method and application of the iron-based nano material in preparation of medicines for treating breast cancer. The iron-based nano material is formed by loading a ferroptosis inducer on a nano carrier; the nano carrier is an iron-based metal organic framework material Fe-MOF (Metal Organic Framework). A product FEH for treating breast cancer is obtained based on coupling of the iron-based nano-material provided by the invention with a targeted drug such as trastuzumab. The FEH provides a new method for developing a nano platform with an antibody function, and realizes a treatment strategy of trastuzumab and ferroptosis combined anti-tumor. As a safe and effective tumor treatment strategy, the EFH has huge clinical transformation potential.

The invention discloses a specific cell targeted hybrid nano-delivery carrier and a preparation method and application thereof, and aims to solve the problems of insufficient specificity, off-target risk, low delivery efficiency and the like of an existing drug delivery system. And a bionic hybrid nano delivery platform with a natural targeting function and high drug loading capacity is constructed. The vector retains key receptor molecules and signal markers on a source cell membrane, and can actively identify and target corresponding cells; meanwhile, due to the introduction of the lipidosome, the yield and stability of the material are remarkably improved, and the immune clearance rate is reduced. Experimental results show that the nano-carrier shows good targeting and biocompatibility in various disease models, and has wide clinical application prospects in the aspects of tumor treatment, tissue repair, inflammation control, targeted imaging and the like.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention relates to the technical field of biological pharmacy, and discloses a QS-21 saponin adjuvant as well as a preparation method and application thereof, the adjuvant is a composite liposome and comprises a liposome skeleton composed of distearoyl phosphatidylcholine and cholesterol, and QS-21 saponin, monophosphoryl lipid A and a local anesthetic are jointly entrapped in the liposome skeleton. The problem that high reactogenicity and immunogenicity of potent adjuvants are difficult to consider at the same time is solved, the immunostimulation component and the pain inhibition component are jointly entrapped in the same nano-carrier, collaborative delivery in injection local is achieved, and therefore pain and swelling caused by the adjuvants are accurately inhibited. The co-entrapment structure avoids the potential inhibition effect of the free anesthetic on the immune system, the potent body fluid and cellular immune enhancement activity of the adjuvant is completely reserved, and a new technical scheme is provided for developing vaccines with high safety and strong immune efficacy.

The invention discloses a binary flooding strong emulsifying active agent based on a nano silicon dioxide grafted anionic-nonionic structure as well as a preparation method and application of the binary flooding strong emulsifying active agent, and belongs to the field of active agents. The binary flooding strong emulsifying active agent based on the nano silicon dioxide grafted anionic-nonionic structure comprises a nano silicon dioxide core, an alkyl chain hydrophobic middle layer and a sulfonate-polyether bifunctional outer layer, the alkyl chain hydrophobic intermediate layer is grafted to the surface of SiO2 through a silane coupling agent by a Si-O-Si covalent bond; and the sulfonate-polyether bifunctional outer layer is composed of a sulfonate group-SO3H and a polyoxypropylene-polyoxyethylene PO-b-EO block copolymer. According to the technology, a traditional physical compounding mode is broken through, a sulfonate group and a polyether chain segment are accurately anchored to a nano-carrier through chemical bonds, molecular-level synergy of anion and nonionic functions is achieved, and the system is endowed with ultralow interfacial tension, strong emulsifying capacity and long-acting stability while the consumption of sulfonate is reduced.

The invention discloses a frontalon tooth healing targeting nano preparation and a preparation process thereof, belongs to the technical field of nano medicines, and aims to solve the problems that the natural regeneration capacity of teeth is limited and the combination of ethnic drugs and modern biotechnology is caused due to the lack of a targeting inhibition means for USAG-1. Comprising an anti-USAG-1 monoclonal antibody, ethnic drug components and a nano-carrier, the anti-USAG-1 monoclonal antibody is connected to the surface of the nano-carrier through covalent bonds, the ethnic drug components are loaded in the nano-carrier through physical action, and the nano-carrier is a polylactic acid-glycolic acid copolymer or chitosan; the anti-USAG-1 monoclonal antibody is used as a targeting ligand, is high in targeting property, can accurately reach a tooth focus, improves the drug utilization rate, enhances the curative effect, integrates multiple ethnic drug components, exerts a synergistic effect, can increase the contact area between the ethnic drug components and a biological membrane, promotes the absorption and transport of the drug, and improves the bioavailability of the drug. And the medicine can better play a treatment role.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention discloses a special antibacterial and antioxidant biosensing nano-composite preservative film for fermented food in the technical field of biochemistry, GOx and Lac are synergistically loaded on a nano-carrier to construct a multifunctional composite film, the loading capacity and catalytic efficiency of enzyme are remarkably improved by the high specific surface area and surface active sites of the nano-carrier, and the antibacterial and antioxidant biosensing nano-composite preservative film can be applied to the field of biochemistry. Meanwhile, the synergistic effect of the two enzymes endows the composite membrane with multiple functions: GOx catalyzes glucose to oxidize into hydrogen peroxide (H2O2), Lac catalyzes and oxidizes polyphenol substances, and the combination of GOx and Lac can realize antibacterial, antioxidant and biosensing functions at the same time. In addition, due to the introduction of the porous structure and the matrix material of the nano composite membrane, the mechanical property and the biocompatibility of the membrane are further enhanced, and the application potential of the membrane in food packaging, medical dressing and biosensors is widened.

The invention relates to the technical field of antibiosis, and discloses a traditional Chinese medicinal material antibacterial component compounding method with a nano-particle self-assembly behavior, which comprises the following steps: S1, a traditional Chinese medicinal material component extraction framework step for extracting an antibacterial component extract from lithospermum erythrorhizon and moutan bark medicinal materials; s2, a step of preparing a nano-particle carrier framework, wherein the nano-particle carrier framework is used for constructing a nano-carrier system composed of chitosan and sodium hyaluronate; s3, an antibacterial component compounding frame step: carrying out self-assembly compounding on the antibacterial component extract and the nano-particle carrier to generate antibacterial nano-composite particles; and S4, a composite particle drying frame step: treating the composite particles through centrifugation and freeze drying to obtain the antibacterial nano composite powder. The long-time sustained release of the antibacterial effect is realized by encapsulating the antibacterial component through the nano composite carrier, optimizing the particle size and adopting a sustained release technology, the stability and bioavailability of the medicine are improved, and side effects caused by excessive release of the medicine are avoided.

The invention relates to a silicon-based nano material for targeted therapy of brucellosis as well as a preparation method and application of the silicon-based nano material. The invention relates to a preparation method of a silicon-based nano material for targeted therapy of brucellosis. The preparation method comprises the following steps: (1) preparing magnetic mesoporous silica; (2) aminating the magnetic mesoporous silica, and modifying the magnetic mesoporous silica with a responsive material and yeast beta-glucan to obtain modified magnetic mesoporous silica; and (3) carrying out antibiotic loading on the modified magnetic mesoporous silica. According to the silicon-based nano material for targeted therapy of brucellosis as well as the preparation method and the application of the silicon-based nano material, the surface of magnetic macroporous mesoporous silica is modified with yeast beta-glucan capable of targeting M cells and glutathione responsive molecules, and a drug delivery system sensitive to a brucellosis environment is synthesized; the nano-carrier has targeted targeting selectivity for delivering and releasing drugs, the activity, slow release and target cell uptake efficiency of antibiotics are ensured, and the brucellosis treatment is more accurate and efficient.

The method comprises the following steps: dissolving lipoic acid and a drug in an organic solvent to form a homogeneous solution, dropwise adding deionized water while stirring, then adding a reducing agent, initiating lipoic acid polymerization and drug co-assembly in situ at 20-40 DEG C to form a drug-loaded micelle solution, and carrying out freeze drying on the drug-loaded micelle solution to obtain the nano-preparation drug based on polylipoic acid. And dialyzing and purifying to obtain the nano preparation medicine based on polylipoic acid. The preparation method is characterized in that lipoic acid has dual characteristics of a drug carrier matrix and a dynamic crosslinking monomer, carrier synthesis and efficient drug encapsulation are synchronously realized through one-step in-situ polymerization-co-assembly, and the technical bottleneck that a traditional nano-carrier needs pre-synthesis and step-by-step drug loading is broken through. The method has the following practicability: (1) the process is simple and efficient, complex equipment is not needed, and reaction conditions are mild; (2) the drug loading performance is excellent, and the particle size is uniform and adjustable (50-300 nanometers); (3) a polylipoic acid skeleton endows the nano-drug preparation with environmental responsiveness, and intelligent drug release can be realized; and (4) the method is suitable for hydrophobic drugs and compound preparations. The method provides a high-load, high-stability and stimuli-responsive nanocrystallization solution for hydrophobic drug delivery, and has application value in the field of nano-medicines.

The invention belongs to the technical field of pesticides, and particularly relates to azobenzene calix [4] arene derivatives as well as a preparation method and application thereof. The invention discloses a novel azobenzene calix [4] arene derivative prepared by directional modification through an azo reduction reaction based on a calix [4] arene skeleton. The azobenzene calix [4] arene derivative has the characteristics of green environmental protection, accurate and controllable structure and efficient synthesis. After the derivative and a pesticide form a host-guest compound, the activity of the pesticide can be remarkably improved, the bioavailability of the pesticide at an action site is increased, the problem of low efficiency of the traditional pesticide is effectively solved, and the development of target drug resistance is delayed. The technology breaks through the technical bottleneck of nano-carrier environmental toxicity, provides a new thought for developing a multi-target and low-environmental-risk pesticide synergist, and has important significance for realizing pesticide decrement, synergism and resistance treatment.

The invention relates to an HA-APS (at) MSN-PDA-CS-mPEG nano particle and a preparation method of the nano particle. The invention relates to a preparation method of HA-APS (at) MSN-PDA-CS-mPEG nanoparticles. The preparation method comprises the following steps: (1) preparing mesoporous silica microspheres; (2) carrying out amination modification on the mesoporous silica microspheres, so as to obtain MSN-NH2; (3) coating the MSN-NH2 by using a PDA-CS-mPEG (Polyethylene Glycol) copolymer, so as to obtain MSN-PDA-CS-mPEG (Polyethylene Glycol); (4) the MSN-PDA-CS-mPEG is subjected to hyaluronic acid modification, and HA (at) MSN-PDA-CS-mPEG is obtained, and HA (at) MSN-PDA-CS-mPEG is obtained; and (5) loading the astragalus polysaccharide on the HA (at) MSN-PDA-CS-mPEG, so as to obtain the HA-APS (at) MSN-PDA-CS-mPEG nano particles. According to the HA-APS-coated MSN-PDA-CS-mPEG nano particle and the preparation method thereof, mesoporous silica is used as a nano carrier, copolymer micelles formed by PDA, CS and mPEG are coated on the surface of a silicon sphere, then HA modified by differentiated cluster protein C4DD is coated, finally astragalus polysaccharide is loaded, and a nano drug delivery system with a targeting effect is obtained by utilizing layer-by-layer structured modification. And the preparation method is high in universality.

The invention provides a lipid nano-carrier, a pharmaceutical preparation, a preparation method and application. The lipid nano-carrier disclosed by the invention comprises lipid nano-particles coupled with endogenous protein, can reduce unexpected liver accumulation and prolong in-vivo long circulation time, has tumor tropism and can realize extrahepatic targeting.

The invention discloses a plant bactericide and a preparation method thereof, and relates to the technical field of bactericides. Comprising the following components: 30-40 parts of plant source extract nano microcapsules, 5-8 parts of a nano carrier, 3-6 parts of a composite synergist, 4-6 parts of dextran, 1-3 parts of eugenol, 4-8 parts of hydroxypropyl trimethyl ammonium chloride chitosan, 3-5 parts of an auxiliary agent, 50-60 parts of ethanol, 100-150 parts of water and 3-5 parts of an emulsifier. The plant source extract is prepared by extracting the following raw materials in parts by weight: 10-20 parts of peach pit green tangerine peel, 8-15 parts of Chinese prickly ash, 4-6 parts of radix sophorae flavescentis, 3-5 parts of ginkgo leaves, 3-5 parts of garlic, 5-8 parts of eucalyptus leaves and 3-5 parts of sophora alopecuroide. The plant bactericide has the advantages of obvious bactericidal effect, low drug resistance risk and good field control effect.

The invention belongs to the field of pesticide chemistry, and relates to preparation of nano-pesticides, in particular to a nano-pesticide with a two-way conveying function and a preparation method and application of the nano-pesticide. MIL-101 (FeIII, CuII) is used as a pesticide carrier, non-systemic fludioxonil pesticide molecules are loaded in a solution, and then the MIL-101 (CuII, FeIII) MOF nanoparticles loaded with the fludioxonil pesticide are encapsulated with chitosan oligosaccharide in the aqueous solution. The nanometer pesticide preparation prevents fludioxonil pesticide molecules from being released in a solvent in advance, the retention amount of the fludioxonil pesticide on leaf surfaces is increased, the lasting pesticide effect of the fludioxonil pesticide molecules in target crops is improved, the nanometer pesticide preparation is environmentally friendly, and the purposes of disease control, agricultural yield increase and quality improvement are achieved. The nano-carrier provided by the invention can be bidirectionally conveyed in crops through leaf surface pores or root hairs, so that the conveying efficiency of fludioxonil pesticide molecules is improved, and the application scene of the nano-carrier is widened.

The invention discloses application of Aeu8-dsRNA in prevention and control of elephant trunk bean root-knot nematode, and belongs to the technical field of biology. The gene Aeu8 capable of remarkably killing the malloidogyne zeylanica is obtained by screening through an in-vitro dsRNA synthesis technology by taking the malloidogyne zeylanica as an experimental material, the dsRNA and a nano-carrier SPc are compounded according to a specific proportion by adopting a dsRNA and nano-material compounding technology to prepare the biological pesticide nano-preparation, and a potted plant root-irrigation experiment finds that the gene Aeu8 can be used for remarkably killing the malloidogyne zeylanica and the nano-carrier SPc can be used for killing the malloidogyne zeylanica. Compared with single use of the dsRNA, the purpose of preventing and controlling the elephant trunk bean root-knot nematode is remarkably improved. According to the invention, a new target gene is provided for screening of pesticide for preventing and controlling the elephant trunk bean root-knot nematode, a new biological pesticide nano preparation combination is provided for preventing and controlling the elephant trunk bean root-knot nematode, and a new method is provided for effectively preventing and controlling the elephant trunk bean root-knot nematode.

The invention provides a POCD treatment medicine composition with dendrobine delivered by a nano-carrier and application, and belongs to the technical field of medicine. The composition consists of dendrobine, a nano-carrier material and a stabilizer, wherein a nano-carrier is PLGA (poly (lactic-co-glycolic acid)) nanoparticles or lipidosome. The particle size of the composition is 50-150nm, the polydispersity index is less than 0.3, and the loading capacity of dendrobine is 5-15%. The invention also provides two preparation methods of the composition. The PLGA nanoparticles are prepared by adopting an emulsification-solvent evaporation method; the liposome is prepared by adopting a film hydration-ultrasonic extrusion method, and each process parameter is specifically limited. The pharmaceutical composition can significantly improve the brain targeting and bioavailability of dendrobine, and can be used for preparing drugs for preventing or treating postoperative cognitive impairment (POCD), and the administration route can be intravenous injection or nasal administration.

The invention discloses a nuclear targeting peptide modified DNA nano-particle based on a microfluidic technology and a preparation method and application thereof, and relates to the technical field of biological medicine, a poly (beta-amino ester) cationic polymer is used as a gene delivery carrier, the gene delivery carrier comprises a microtubule related sequence MTAS and a nuclear localization signal NLS oligopeptide, the gene delivery carrier and the plasmid DNA carrying the target gene are mixed to prepare nano-particles, and the plasmid DNA carrying the target gene is uniformly dispersed in the nano-particles by adopting a microfluidic mixing process; the nuclear targeting peptide modified DNA nano-particle is ingenious in structural design, the mixing uniformity of a nano-carrier and plasmid DNA is improved by adopting a micro-fluidic mixing process, the performance of the nano-particle is further improved by optimizing dialysis conditions, potential cytotoxicity and adverse effects are reduced, and the nuclear targeting peptide modified DNA nano-particle is suitable for large-scale production. And after transduction of non-activated T cells, strong tumor killing ability and cytokine secretion can be generated.

The invention discloses a monascus red pigment nano stabilized carrier constructed based on a hierarchical self-assembly strategy as well as a preparation method and application thereof, and belongs to the technical field of nano carriers. According to the invention, a beta-cyclodextrin-enzymolysis sodium caseinate-Arabic gum ternary supramolecular composite system is constructed, and efficient encapsulation, bitter taste shielding and stability improvement of pigment molecules are realized by virtue of a multi-stage self-assembly mechanism. According to the carrier, a hydrophobic core domain is constructed by virtue of an enzymolysis peptide fragment, bitter components are captured by virtue of an inclusion effect of beta-cyclodextrin, and the interface stability is enhanced by virtue of Arabic gum, so that a nano-scale delivery system with a clear structure and excellent performance is finally formed, and the photo-thermal stability and sensory quality of the pigment are remarkably improved; and the powder obtained by spray drying is good in flowability, easy to re-disperse and high in product applicability. The carrier is wide in raw material source, the preparation process is green and mild, large-scale amplification is easy, and the carrier has good application potential in the high-value-added fields such as high-end functional food, clarified beverages and pharmaceutical preparations.

The present invention discloses a method for batch inoculation of phages to control tobacco bacterial wilt, including: Step S1, culturing phages and Ralstonia solanacearum together to obtain phages with the ability to infect and lyse tobacco Ralstonia solanacearum; Step S2, encapsulating the phages into a nano-carrier to prepare nanoparticles, where the nano-carrier is a biodegradable nano-carrier; Step S3, cutting off water for hardening seedlings 5 - 7 days before transplanting flue-cured tobacco floating seedlings. After the hardening of seedlings is completed, put 2 - 3 cm of shallow water in the seedling-raising pool, add the phage-containing nanoparticles and an adhesive to the nutrient pool, and put the floating seedling trays back into the seedling-raising pool for inoculation. By combining phage therapy and nanotechnology, the present invention adds nano-encapsulated phages to tobacco floating seedling raising, which can not only inoculate a large number of tobacco seedlings more conveniently and quickly, but also extend the persistence of phages in the soil and improve their control efficacy.

The invention discloses a tumor vaccine of a targeted delivery system and a preparation process of the tumor vaccine, and belongs to the field of biological medicine, the tumor vaccine comprises a self-assembled nano-carrier, the self-assembled nano-carrier is formed by self-assembling a biodegradable polymer and an amphiphilic block copolymer, and the self-assembled nano-carrier can be spontaneously assembled into a nano-structure in an aqueous solution according to environmental conditions; the multiple targeting molecules are combined on the self-assembled nano-carrier, and the targeting molecules comprise at least one of a tumor specific antibody, a tumor vascular endothelial growth factor receptor ligand and an aptamer of tumor cell surface overexpression protein; the delivery efficiency of the tumor vaccine is remarkably improved by the multi-targeting mechanism and the optimized nano-carrier; the immunoreaction intensity is effectively improved; the traditional multi-step synthesis is simplified into one-step rapid synthesis through a preparation process combining an ultrasonic-assisted solvent evaporation method and a microfluidic liquid-liquid phase distribution method, so that the production time is shortened.

The application discloses a drug-loaded micelle capable of effectively crossing the blood-brain barrier, which is an amphiphilic conjugate composed of a reduction-sensitive paclitaxel prodrug and a nucleic acid complex, wherein the reduction-sensitive paclitaxel prodrug is used as a hydrophobic part, and the nucleic acid complex is used as a hydrophilic part, and the drug-loaded micelle is self-assembled in an aqueous environment; the reduction-sensitive paclitaxel prodrug is formed by the reaction of paclitaxel and a disulfide bond-containing linker; and the nucleic acid complex is formed by connecting an antisense oligonucleotide and an interfering RNA through a DNA bridge. The drug-loaded micelle exhibits superior blood-brain barrier penetration, effectively realizes enrichment in brain tumors, solves the problem of low blood-brain barrier penetration efficiency of existing nano-carriers, and provides an effective basis for brain drug delivery and brain diseases such as brain tumor imaging and treatment.

The invention discloses an oligomeric amino acid dendritic macromolecular material as well as a preparation method and application thereof, and relates to the technical field of nano materials. The general formula of the oligomeric amino acid dendritic macromolecular material is TPE-(PEG) n-(Lys) x-(Arg) y, wherein TPE is a hydrophobic core, has an aggregation-induced emission characteristic, and can enhance the self-assembly capability and the tracing function of the nano-carrier; pEG is a connecting arm, so that the water solubility and biocompatibility of the material can be improved; lys is a branch skeleton and is a main construction unit of a dendritic macromolecule; arg is a functional terminal, guanidyl of Arg has strong positive charges, electrostatic binding with negatively charged nucleic acid can be promoted, and meanwhile cellular uptake can be enhanced possibly through the membrane penetration effect. The oligomeric amino acid dendritic macromolecular material gives consideration to stability, modifiability and targeting, provides a new scheme for small nucleic acid delivery, and is expected to have far-reaching influence in the fields of precise tumor treatment, gene therapy and multi-modal diagnosis and treatment.

The invention discloses a spleen-targeted nano platform construction method suitable for tumor vaccination, and particularly relates to the field of vaccines.The spleen-targeted nano platform construction method comprises the steps that S1, CL15H6-DOPS LNPs, spherical polymer vesicles and erythrocyte membrane coated nano-particles are selected as spleen-targeted nano-carriers; s2, integrating an immunologic adjuvant; mn < 2 + > doped LNPs, ultrasonic response lipidosome and a CD3 antibody are selected to be coupled to serve as an integration material; s3, delivering in vivo; animal models are selected for in-vivo delivery and curative effect verification, and B16F10 melanoma mice, MC38 colon cancer mice and non-human primates are selected as the animal models respectively. By optimizing the chain length and the surface topological structure (such as spherical polymer vesicles) of the liposome, the spleen enrichment rate is remarkably increased, and the red marrow myeloid cell uptake efficiency is enhanced. By combining with common delivery of a manganese adjuvant, an STING channel is activated, secretion of type I interferon is promoted, and the proportion of antigen-specific CD8 + T cells and the tumor inhibition rate are increased.