399 results about "Nano carriers" patented technology

The invention provides a catalyst with high catalytic activity, selectivity and stability, namely supported imidazole ion liquid cross-linked polymer nano particles, and belongs to the technical field of chemical synthesis. Experiments prove that the catalyst for the reaction of carbon dioxide and epoxy compound for generating a five-member ring compound has high selectivity (100 percent) and conversion rate (99.8 percent) and can be reclaimed and reused. In addition, a preparation method for the catalyst is simple and has mild reaction conditions, the catalyst is easily separated from a product by using a filter method, and any stabilizer or surfactant is not added in the synthesis process. The catalyst is suitable for large-scale production, and has wide application prospect in the fields of polymer nano carriers, industrial catalysis and the like.

The invention belongs to the field of immobilization of proteins, and relates to preparation and application of a dopamine functional magnetic nano-carrier. The preparation comprises the following steps: firstly, dissolving FeSO4.4H2O and FeCl3.6H2O into distilled water, high-speed stirring and under protection of nitrogen, adding ammonia water of 25% NH4OH, and adding oleic acid; secondly, repeatedly cleaning black precipitates obtained by a stirring reaction and then using a strong magnet to perform precipitation separation, and drying to obtain oleic acid magnetic nano-particles; and finally, adding the oleic acid magnetic nano-particles into a dopamine hydrochloric acid solution, after the reaction is over, using a magnet to separate, cleaning and drying obtained deep brown precipitates, and then the dopamine functional magnetic nano-carrier can be obtained. According to the preparation and application of the dopamine functional magnetic nano-carrier provided by the invention, dopamine hydrochloride is adopted for modifying magnetic oleic acid nano-particles, so that the surface of a magnetic nano-particle has a plentiful of quinonyls and amidogen functional groups, thus proteins can be effectively immobilized; besides, the surface of the carrier has a layer of soft poly dopamine oxide films, the surface area is larger, and the preparation method is simple.

The invention discloses drug-carrying liposome co-modified by folic acid and TAT peptide and a preparation method thereof. The drug-carrying liposome comprises liposome, a long chain targeted membrane material, a short chain targeted membrane material and a drug. Meanwhile, the invention provides the preparation method of the drug-carrying liposome co-modified by folic acid and TAT peptide. The method comprises the following steps: weighing phospholipid, cholesterol, the long chain targeted membrane material, the short chain targeted membrane material and the drug, dissolving the components in an organic solvent, and then carrying out rotary evaporation at 50 DEG C under reduced pressure to remove the organic solvent so as to obtain a medicated liposome membrane; adding a phosphate buffer solution into the medicated liposome membrane for dissolving, carrying out ultrasonic treatment for 2 minutes, and filtering for 10 times by using a 0.22mu m membrane to obtain double-target drug-carrying liposome. According to the drug-carrying liposome disclosed by the invention, the TAT peptide is connected with specific ligands by means of PEG with different weight-average molecular weights to establish a nanometer carrier modified by double ligands, and the prepared drug-carrying liposome can be used for efficiently conveying the drug in tumor cells.

The invention relates to a heptamethine cyanine active fluorescent probe and a preparation method and application thereof. The structural formula of the heptamethine cyanine active fluorescent probe is as shown in the specification, wherein X=II-IX; each of R1 and R2 is (CH2)mCH3, (CH2)nOH, (CH2CH2O)pCH3 and CH2C6H5; each of R3 and R4 is H, SO3H, SO3Na and SO3K; each of a, b, c, d, e, f and g is 2-8; each of n, m and p is 1-10. The heptamethine cyanine active fluorescent probe has the advantages that the heptamethine cyanine active fluorescent probe is based on near-infrared long-wave heptamethine cyanine dye, indoline is selected as the aroma parent nucleus to increase fluorescence intensity, and methenyl chain intermediate cyclohexene rigid bridging enhances stability; nitrogen derivatives with chemical reactivity sites are used to perform nucleophilic substitution on the meso-position of the heptamethine cyanine parent dye, and accordingly Stokes shift and active chemical groups areincreased greatly to facilitate the fluorescent labeling of various substances; the fluorescent probe is of a symmetrical structure, preparation and purification processes are simplified, and cost islowered favorably; the probe can be used as the fluorescent labeling probe of biological molecules such as high-sensitivity protein, sugar and DNA and nano carriers to perform cell or living-body horizontal fluorescence imaging, and the like.

The invention relates to the fields of macromolecular chemistry and medicinal adjuvant, in particular to an amphoteric ion-based charge reversal chitosan derivative and application thereof in a medicament. The chitosan derivative is characterized in that chitosan is used as a framework, and 2-NH2 of the chitosan is grafted with hydrophilic succinyl group, histidine and hydrophobic long-chain octyl; or 2-NH2 of the chitosan is grafted with hydrophilic lysine and hydrophobic chain octyl, and then succinyl group or citryl playing a role in charge reversal is introduced into the 2-NH2 of the chitosan and the -NH2 of the lysine. The chitosan derivative of the invention has the effect of solubilizing insoluble medicaments; and the surface of the charge reversal chitosan derivative carries negative charges in long circulation in vivo, and the charges are reversed to form positive charges by responding to the weak acid environment condition after reaching the outside of tumor cells or the inside of lysosome, so the chitosan derivative can promote the cells to take in nano carriers and promote escape from the lysosome, improve the delivery efficiency of the medicament and reduce the toxic or side effect of the medicament.

The invention relates to an amphiphilic triblock copolymer, a polymer nano-carrier preparation and preparation methods. The copolymer is a liner polymer compound containing a polyethylene glycol derivative, poly-lysine and poly-leucine, wherein one end of the poly-lysine is connected with the polyethylene glycol derivative through an amide bond, and the other end of the poly-lysine is connected with the poly-leucine through a peptide bond. The amphiphilic triblock copolymer, namely the polyethylene glycol derivative-poly-lysine-poly-leucine triblock copolymer combines the advantages of polyethylene glycol and poly-amino acid, can form a nano-carrier with a three-layer structure by self-assembly in a water solution, and can simultaneously effectively load a small molecular hydrophobic medicament, gene substances and proteins or polypeptides to form a multifunctional nano-carrier.

The invention belongs to the field of enzyme immobilizing, and specifically relates to a method for immobilizing enzymes by using a magnetic ionic liquid composite material. According to the invention, the method comprises the steps that: (1) magnetic nano-particles are prepared; (2) ionic liquid is loaded in the magnetic nano-particles, such that the magnetic ionic liquid composite material is obtained; (3) enzymes are immobilized by using the magnetic ionic liquid composite material. According to the invention, the ionic liquid is loaded in the magnetic nano-carriers, such that a magnetic ionic liquid composite material enzyme immobilizing system is formed. With the method, an ionic liquid microenvironment is introduced, and the ionic liquid has almost no vapor pressure, such that the method has environmental protecting characteristic and designability. With the method, enzyme activity and stability can be improved. According to the invention, a nano-material is used as a carrier in enzyme immobilizing, such that the dispersibility of a catalyst can be improved.

The invention relates to a nano-carrier loaded with a rhodiola rosea extract. The nano-carrier comprises the following components according to the weight ratio: 0.5% to 3% of rhodiola rosea extract, 20% to 30% of solvent, 40% to 50% of emulsifier and 18% to 35% of lipid material; the lipid material is a solid and liquid mixture of a liquid lipid material and a solid lipid material, and the emulsifier comprises a water-in-oil emulsifier and an oil-in-water emulsifier. A preparation method of the nano-carrier with the rhodiola rosea extract comprises the following steps: mixing the lipid material and the emulsifier as an oil phase; adding the water-in-oil emulsifier into the solvent as an internal aqueous phase; adding internal aqueous water into a liquid state oil phase; uniformly mixing and solidifying after being cooled to room temperature; adding the water-in-oil emulsifier, uniformly mixing to obtain a lipid system, solidifying after being cooled to room temperature to obtain the nano-carrier loaded with the rhodiola rosea extract. The invention has the following advantages: the nano-carrier loaded with the rhodiola rosea extract is jointly utilized with a technology of multiple emulsion and lipid nanoparticles, the traditional liquid state oil is replaced by the mixture of the solid state oil and the liquid state oil, the internal aqueous phase as the rhodiola rosea extract is more stable than the traditional multiple emulsion, and the obtained nano-carrier loaded with the rhodiola rosea extract as a material can be added into the cosmetics and foods, so that the existing application defects are effectively solved.

The present invention features compositions in which nano-carriers are synthesized with polymers that respond to lower pH and/or ROS by being degraded. The compositions may be utilized to selectively deliver payloads within patients by responding to lower pH and/or ROS at localities within the patient. The present invention also features methods of synthesizing nano-carriers that are degraded by lower pH and/or ROS.

The invention provides a bionic binary cooperative nano-carrier as well as a preparation method and an application thereof. The bionic binary cooperative nano-carrier comprises an erythrocyte membrane, glucose oxidase, iron-supporting ferritin nano-particles and a photosensitizer, wherein the glucose oxidase and the iron-supporting ferritin nano-particles are coated with the erythrocyte membrane,and the photosensitizer is embedded into the surface of the erythrocyte membrane or entrapped by the erythrocyte membrane. Chain stimulative responsibility coordination of tumor hunger therapy and chemical kinetic therapy is realized, two enzymes are conveyed to a target site of an organism with the carrier on the basis of biocompatibility of the erythrocyte membrane and tumor targeting of targeting molecules, accurate administration is realized by membrane rupture based on 808 nm near-infrared light illumination in the tumors, the problem of drug resistance is solved effectively, furthermore,systemic toxicity caused by drug application is remarkably reduced, and damage to other normal tissue in an in-vivo circulation process is prevented effectively. The invention further provides the preparation method of the bionic binary cooperative nano-carrier. The bionic binary cooperative nano-carrier and the preparation method have good application prospect.

The invention relates to a preparation method and application of a multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system which integrates tumor targeting, pH sensitivity, visualization and the like. The core of the siRNA nano carrier system consists of cadmium telluride quantum dots. A nano carrier shell consists of a tumor targeted gene, a 9 poly-l-arginine peptide chain and PEG (Polyethylene Glycol). The siRNA nano carrier is good in biocompatibility, strong in tumor targeting and high in cell transfection efficiency, and has the characteristics of high quantum yield, strong optical stability, lossless in-position real-time monitoring and the like, so that the system is suitable for being used as a nano transmission system for in vivo gene therapy of tumors.

The invention belongs to the technical field of biomedical polymer material and nanobiomaterial, particularly relates to a magnetic nano-carrier with targeted hydrophobic drug delivery to tumor and a preparation method thereof. The magnetic nano-carrier is formed by encapsulating superparamagnetic nano-particles with amphiphilic segmented copolymer methoxy polye thylene glycol-poly(lactide- glycolide) (MePEG-PLGA) micelles. First, a large amount of MePEG-PLGA with good dispersion is synthesized by the improved solution polymerization process, and then the MePEG-PLGA is used as raw materials to encapsulate Fe3O4 nano-particles by the solvent evaporation process to obtain a magnetic nano drug carrier. The carrier is expected to solve the solubility problem of insoluble drugs, achieves slow release of drugs, and achieves the passive-targeting of the drugs by the EPR effect of tumor; Fe3O4 nano-particles are used in the active-targeting of the drugs to reduce the dosage and the side effect and improve the treatment efficiency; besides, the carrier can be used in the magnetic resonance imaging (MRI) of tumor.

The invention belongs to the field of biological medicine and specifically discloses a nucleic acid-drug conjugate based on phosphorothioate modified nucleic acid, a drug delivery system and a preparation method thereof. Phosphorothioate groups in the phosphorothioate modified nucleic acid react with groups modified on drug molecules and capable of generating electrophilic reaction with the phosphorothioate groups, so as to form the nucleic acid-drug conjugate; the nucleic acid-drug conjugate can be self-assembled into drug-containing nano-carriers in various forms for drug delivery, in the manner of selecting different nucleotide sequences including functional nucleic acid; compared with the prior art, the invention has the advantages that the nucleic acid-drug conjugate can be acquired through a simple solid-phase synthesis technology, grafting sites and assembling forms of drug molecules on nucleic acid skeleton can be accurately controlled and the method has universality to chemotherapeutic drugs; according to the nucleic acid-drug conjugate, the drug delivery system, the preparation method and the application thereof disclosed by the invention, physicochemical properties and in vivo distribution properties of chemotherapeutic drugs are obviously improved, therapeutic effect thereof can be promoted and combination therapy of gene therapy and chemotherapy can be realized.

The invention provides a serial cell-penetrating peptide modified tumor targeted and tumor penetrated nano drug delivery system capable of simultaneously identifying an integrin receptor and a transmembrane protein receptor, wherein the serial cell-penetrating peptide is mainly formed by connecting a DGR polypeptide fragment covalently at the C-tail end of polyarginine of the cell-penetrating peptide. The polypeptide not only can selectively identify two highly expressed receptors on the surfaces of the tumor cells, but also can be efficiently mediated into the cells. The nano drug delivery system mainly consists of a nano carrier, a drug and a lipid-polyethylene glycol-serial cell-penetrating peptide ligand interstitial compound and is a potential tumor targeted treatment carrier.

The invention provides a targeting nano carrier bearing nucleoside anti-tumor drugs and preparation method and application thereof. The targeting nano carrier comprises DNA tetrahedron, Affibody molecule and nucleoside anti-tumor drugs. The preparation method comprises the following steps: a, the nucleoside anti-tumor drugs are modified with phosphoramidite; B, synthesizing four DNA single strandsby DNA solid phase synthesis technology, integrating the nucleoside anti-tumor drugs into the 5 'ends of the four DNA single strands, and modifying NH2 at the 3' ends of one of the DNA single strands; C, linking the affibody molecule to the 3 'end of the NH2-modified DNA single strand through a linker; (d) mix and assembling that four DNA single strand in a molar ratio of 1: 1: 1: 1 to obtain DNAtetrahedron; and (d) assembling the four DNA single strands in a molar ratio of 1: 1: 1 to obtain DNA tetrahedron; Affibody targeting nanoparticles. The targeting nano carrier of the invention can precisely control the combined quantity of drugs, has faster tumor targeting ability and higher cell, tissue penetration ability, good pharmaceutical effect, and is suitable for popularization and application.

The invention relates to the technical field of medicines, and relates to adriamycin-indocyanine green bionic nanoparticles coated with platelet and neutrophil fusion membranes and an application of the adriamycin-indocyanine green bionic nanoparticles in preparation of medicines for treating tumor metastasis diseases. The bionic nanoparticle coated with the platelet and neutrophil hybrid membranecomprises adriamycin, indocyanine green, a nano carrier material, a platelet membrane and a neutrophil hybrid membrane, which is characterized by comprising the following components in percentage byweight: 8 to 10 percent of doxorubicin, 8 to 10 percent of indocyanine green, 30 to 40 percent of nano carrier material and the balance of platelet and neutrophile granulocyte hybrid membrane. The bionic nanoparticle has the capability of simultaneously capturing and removing circulating tumor cells and tumor-derived exosomes through a high-affinity membrane adhesion receptor, and effectively cutsoff the relationship between the exosomes and immune cells. The primary tumor can be completely ablated, and breast cancer metastasis can be efficiently inhibited in xenograft and in-situ breast tumor models.

The invention discloses a nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of the nano-drug system. The nano-drug system takes polydopamine (PDA) as a nano-carrier, and pentacarbonyl manganese bromide and the DOX are supported on the PDA. The preparation method of the nano-drug system is simple. Dopamine hydrochlorideis dispersed into double-distilled water to obtain a dopamine hydrochloride solution, a NaOH solution is added, repeated centrifugal separation is carried out to obtain PDA, the PDA is dispersed in the double-distilled water, an ethanol solution of pentacarbonyl manganese bromide and the double-distilled water solution of DOX are added under violent stirring, rotary stirring is carried out at room temperature to obtain a black solution, a supernatant is removed by centrifugal separation, and an obtained precipitate is PDA-DOX-CO nanoparticles. The nano-drug system can controllably release theCO and the DOX under near-infrared light irradiation, effectively inhibit tumor growth, and has a good long-circulation function, biocompatibility and stability.

The invention provides self-detection anticorrosive paint with nano-carriers, a preparation method of the paint and an application. The self-detection anticorrosive paint comprises a self-detection color material, the nano-carriers and a film-forming substrate. The method for preparing the self-detection anticorrosive paint with the nano-carriers includes the steps: firstly, preparing the nano-carriers, and configuring the nano-carriers into nano-carrier water solution with a certain proportional concentration; secondly, mixing the nano-carrier water solution and the self-detection color material in proportion to obtain first mixed liquid; thirdly, performing ultrasonic dispersion for the first mixed liquid to obtain second mixed liquid; fourthly, drawing, filtering and removing unreacted solution in the second mixed liquid to obtain a solid matter, and adding water into the solid matter to form third mixed liquid with a certain proportion by matching; fifthly, mixing the third mixed liquid and the film-forming substrate to obtain the anticorrosive paint. The self-detection anticorrosive paint is environmentally friendly and has the functions of automatic detection, early warning and corrosion prevention.

The invention discloses a lipoic acid modified chondroitin sulfate-chlorin e6 amphoteric polymer. According to the polymer, chondroitin sulfate is used as a water-soluble framework material, chlorin e6 serves as a hydrophobic modifier to be connected into a chondroitin sulfate framework, lipoic acid is used as an interface cross-linking agent to conduct surface modification on a chondroitin sulfate-chlorin e6 polymer, and the polymer further has a hydrophobic core to be capable of entrapping an insoluble anti-tumor drug. The lipoic acid modified chondroitin sulfate-chlorin e6 amphoteric polymer prepared by the invention is used as a drug carrier to prepare reversible cross-linked nano-micelles, and thus rapid decrosslinking in tumor cells can be realized; and as an excellent nano-carrier,sonodynamic therapy (SDT) of the tumors is achieved, and the lipoic acid modified chondroitin sulfate-chlorin e6 amphoteric polymer has the effects of increasing the level of active oxygen in the cells, promoting tumor cell apoptosis and remarkably inhibiting tumor cell growth. Meanwhile, the hydrophobic core provides entrapment of the anti-tumor drug, combined application of chemotherapy and SDTof the tumors can be achieved, and the anti-tumor effect is expected to be improved.

The invention relates to preparation of a receptor targeted nano metal organic framework and an application of the preparation. The invention aims at solving the problems that the existing carrier is low in drug loading rate and poor in targeting. The preparation method provided by the invention comprises the following steps: 1, preparing a nano metal organic framework; and 2, preparing a RGD (arginine-glycine-aspartic acid) modified nano metal organic framework. The receptor targeted nano metal organic framework provided by the invention is applied in drug carriers. The synthesis route of an organic metal framework is changed to prepare the nano metal organic framework having the advantages of nano carriers; when being used as a drug carrier, the RGD modified nano metal organic framework has good targeting and large drug loading rate, the carrier of the receptor targeted nano metal organic framework is loaded by means of the adsorption property of specific surface area of a mesoporous carrier, and the loading quantity can reach 50%. The receptor targeted nano metal organic framework provided by the invention is applied in the pharmaceutical field.

The invention relates to a preparation technology and a production method of a novel integrated dosage form of Shuquan pills. The raw material composition of the active ingredients is as follows: 9 parts of bird medicine, 9 parts of yam, and 9 parts of Yizhi kernel. Its production process includes: supersonic jet pulverization, alcohol water extraction, ultrasonic pulverization and extraction, water decoction and concentration, supersonic spray drying, nano grinding, high pressure emulsification, nano particle preparation, etc. The invention pays attention to the advantages of multi-synergy and multi-targeting embodied by the combination technology of nano-carriers. After large-scale production, the production cost can be greatly reduced, the product quality can be greatly improved, and the targeting and sustained release of the drug can be strengthened. It can be administered both internally and externally, and can be taken in four time periods according to the meridian flow and the flow of blood in the human body. Film-forming agents and transdermal agents can also be prepared and pasted on different parts of the human body according to the meridian flow and the flow of human Qi and blood, and can be directly absorbed through the skin.