165 results about "Nano carriers" patented technology

The invention relates to the technical field of pollution detection, in particular to a pollution assessment method, system, equipment and medium for a refuse landfill, and the method comprises the following steps: firstly, obtaining full-spectrum induced polarization data of the landfill to construct a stratum electrochemical attribute model; resolving a retardation factor distribution field by using the physical and chemical parameters of the pollution carrier and the model; constructing a Darcy velocity field in combination with hydrogeological parameters, and generating a pollution carrier migration velocity field through retardation factor correction; and finally, streamline tracing simulation is executed based on the velocity field, so that a target monitoring well coordinate and a risk early warning level are output. The technical problems that the dominant flow channel of the structural fracture is difficult to identify under the complex geology and evaluation distortion is caused by non-synchronous migration caused by the interface electrochemical action of the micro-nano carrier in the traditional method are solved; accurate positioning of the hidden leakage channel and accurate prediction of the penetration time are achieved, and the early warning capacity and the management and control efficiency of landfill environment risks are remarkably improved.

The invention discloses an engineered bionic nucleic acid nano-vesicle as well as a preparation method and application thereof, relates to the technical field of nano biomedicine, and aims at solving the problems that in-vivo targeting efficiency and immunogenicity of a traditional cationic lipid nano-carrier are limited due to deletion and cleavage obstacles of GSDMD expression in tumor cells. The technical key point of the invention is as follows: the engineered bionic nucleic acid nano-vesicle is provided and is prepared by wrapping a cationic lipid nucleic acid drug with an exosome derived from engineered macrophages; wherein the exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1, which is as shown in SEQ. ID. NO.1. The exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1; the lipid nucleic acid medicine is prepared by loading GSDMD-N mRNA (messenger Ribonucleic Acid) shown on the basis of SEQ.ID.NO.2 on a cationic liposome. The engineered bionic nucleic acid nano-vesicle is used for preparing an oral squamous cell carcinoma diagnostic kit and a therapeutic drug.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention relates to the technical field of biological pharmacy, and discloses a QS-21 saponin adjuvant as well as a preparation method and application thereof, the adjuvant is a composite liposome and comprises a liposome skeleton composed of distearoyl phosphatidylcholine and cholesterol, and QS-21 saponin, monophosphoryl lipid A and a local anesthetic are jointly entrapped in the liposome skeleton. The problem that high reactogenicity and immunogenicity of potent adjuvants are difficult to consider at the same time is solved, the immunostimulation component and the pain inhibition component are jointly entrapped in the same nano-carrier, collaborative delivery in injection local is achieved, and therefore pain and swelling caused by the adjuvants are accurately inhibited. The co-entrapment structure avoids the potential inhibition effect of the free anesthetic on the immune system, the potent body fluid and cellular immune enhancement activity of the adjuvant is completely reserved, and a new technical scheme is provided for developing vaccines with high safety and strong immune efficacy.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention provides a POCD treatment medicine composition with dendrobine delivered by a nano-carrier and application, and belongs to the technical field of medicine. The composition consists of dendrobine, a nano-carrier material and a stabilizer, wherein a nano-carrier is PLGA (poly (lactic-co-glycolic acid)) nanoparticles or lipidosome. The particle size of the composition is 50-150nm, the polydispersity index is less than 0.3, and the loading capacity of dendrobine is 5-15%. The invention also provides two preparation methods of the composition. The PLGA nanoparticles are prepared by adopting an emulsification-solvent evaporation method; the liposome is prepared by adopting a film hydration-ultrasonic extrusion method, and each process parameter is specifically limited. The pharmaceutical composition can significantly improve the brain targeting and bioavailability of dendrobine, and can be used for preparing drugs for preventing or treating postoperative cognitive impairment (POCD), and the administration route can be intravenous injection or nasal administration.

The invention discloses a monascus red pigment nano stabilized carrier constructed based on a hierarchical self-assembly strategy as well as a preparation method and application thereof, and belongs to the technical field of nano carriers. According to the invention, a beta-cyclodextrin-enzymolysis sodium caseinate-Arabic gum ternary supramolecular composite system is constructed, and efficient encapsulation, bitter taste shielding and stability improvement of pigment molecules are realized by virtue of a multi-stage self-assembly mechanism. According to the carrier, a hydrophobic core domain is constructed by virtue of an enzymolysis peptide fragment, bitter components are captured by virtue of an inclusion effect of beta-cyclodextrin, and the interface stability is enhanced by virtue of Arabic gum, so that a nano-scale delivery system with a clear structure and excellent performance is finally formed, and the photo-thermal stability and sensory quality of the pigment are remarkably improved; and the powder obtained by spray drying is good in flowability, easy to re-disperse and high in product applicability. The carrier is wide in raw material source, the preparation process is green and mild, large-scale amplification is easy, and the carrier has good application potential in the high-value-added fields such as high-end functional food, clarified beverages and pharmaceutical preparations.

The application discloses a drug-loaded micelle capable of effectively crossing the blood-brain barrier, which is an amphiphilic conjugate composed of a reduction-sensitive paclitaxel prodrug and a nucleic acid complex, wherein the reduction-sensitive paclitaxel prodrug is used as a hydrophobic part, and the nucleic acid complex is used as a hydrophilic part, and the drug-loaded micelle is self-assembled in an aqueous environment; the reduction-sensitive paclitaxel prodrug is formed by the reaction of paclitaxel and a disulfide bond-containing linker; and the nucleic acid complex is formed by connecting an antisense oligonucleotide and an interfering RNA through a DNA bridge. The drug-loaded micelle exhibits superior blood-brain barrier penetration, effectively realizes enrichment in brain tumors, solves the problem of low blood-brain barrier penetration efficiency of existing nano-carriers, and provides an effective basis for brain drug delivery and brain diseases such as brain tumor imaging and treatment.

The invention discloses an oligomeric amino acid dendritic macromolecular material as well as a preparation method and application thereof, and relates to the technical field of nano materials. The general formula of the oligomeric amino acid dendritic macromolecular material is TPE-(PEG) n-(Lys) x-(Arg) y, wherein TPE is a hydrophobic core, has an aggregation-induced emission characteristic, and can enhance the self-assembly capability and the tracing function of the nano-carrier; pEG is a connecting arm, so that the water solubility and biocompatibility of the material can be improved; lys is a branch skeleton and is a main construction unit of a dendritic macromolecule; arg is a functional terminal, guanidyl of Arg has strong positive charges, electrostatic binding with negatively charged nucleic acid can be promoted, and meanwhile cellular uptake can be enhanced possibly through the membrane penetration effect. The oligomeric amino acid dendritic macromolecular material gives consideration to stability, modifiability and targeting, provides a new scheme for small nucleic acid delivery, and is expected to have far-reaching influence in the fields of precise tumor treatment, gene therapy and multi-modal diagnosis and treatment.

The invention discloses an esophageal cancer targeting nano-tablet based on a traditional Chinese medicine composition and a preparation method of the esophageal cancer targeting nano-tablet. The targeted nano tablet is prepared from the following refined extracts: a rhizoma paridis total saponin extract, a pseudobulbus cremastrae seu pleiones colchicine derivative extract, a panax notoginseng total saponin extract, a carthamin yellow extract, a thunberg fritillary bulb total alkaloid extract, frankincense-myrrh compound volatile oil, a resin extract and an astragaloside IV extract. The nano-carrier comprises a nano-carrier, a seaweed-laminarin composite extract, a processed rhizoma pinelliae total alkaloid extract and costus root volatile oil, and the costus root volatile oil serves as one of targeting ligands to be used for modifying the nano-carrier. The targeting property is strong: the dual-targeting ligand (costunolide-TOPK affinity peptide) on the surface of the nano-carrier can actively recognize and combine with a specific receptor of esophageal cancer cells, and meanwhile, due to the size (about 100-200 nanometers) of the carrier, the carrier is easy to enrich in tumor tissues through enhanced permeation and retention (EPR) effect, so that the modernization upgrading of the idea of'channel guiding medicine 'is realized.

The invention discloses a preparation method of anti-aging whitening skin cream based on traditional Chinese medicine exosome extraction, and relates to the technical field of cosmetics. Comprising the following steps: (1) raw material compatibility and pretreatment: taking 1-8 parts by mass of abelmoschus manihot exosome, 1-8 parts by mass of bighead atractylodes rhizome exosome, 1-8 parts by mass of bletilla striata exosome, 1-8 parts by mass of radix angelicae exosome, 1-8 parts by mass of white poria cocos exosome, 1-8 parts by mass of radix paeoniae alba exosome, 1-8 parts by mass of lily exosome, 1-8 parts by mass of silkworm larva exosome, 1-8 parts by mass of white asarum exosome and 1-8 parts by mass of cordyceps sinensis exosome; according to the skin care product disclosed by the invention, the exosome system of twelve traditional Chinese medicinal materials is applied to the field of skin care products for the first time; a complete traditional Chinese medicine exosome extraction, identification and preparation technology system is established; the exosome is used as a natural nano-carrier, so that the skin permeability of active ingredients is remarkably improved; based on the theory of complementing color with color in traditional Chinese medicine, traditional Chinese medicinal materials with white character heads are carefully selected; the exosomes of the medicinal materials have a synergistic effect, so that multi-target skin metabolism regulation is realized.

This invention relates to the field of biopharmaceutical technology, disclosing a QS-21 saponin adjuvant, its preparation method, and its applications. The adjuvant is a composite liposome comprising a liposome backbone composed of distearate phosphatidylcholine and cholesterol, with QS-21 saponin, monophosphatidyllipid A, and a local anesthetic co-encapsulated therein. This invention solves the common problem of high reactivity and immunogenicity in potent adjuvants by co-encapsulating immunostimulatory and analgesic components on the same nanocarrier, achieving synergistic delivery at the injection site, thereby precisely inhibiting adjuvant-induced pain and swelling. This co-encapsulation structure avoids the potential inhibitory effect of free anesthetics on the immune system, fully preserving the adjuvant's potent humoral and cellular immune-enhancing activity. This invention provides a new technical solution for developing vaccines with both high safety and strong immunogenicity.

The invention relates to the technical field of food packaging, in particular to a plasma modified polysaccharide intelligent packaging film and a preparation method and application thereof. The plasma modified polysaccharide film forming matter comprises a compound obtained by blending plasma modified polysaccharide and a water-soluble synthetic high-molecular polymer, and a nano-carrier loaded with a natural pH indicator. The invention aims to provide a degradable multifunctional intelligent packaging film which aims to overcome the defect that the stability of a natural indicator and the synergism of a film-forming substrate are insufficient. The method mainly solves the problems of degradation and migration of anthocyanin (ACNs) under the interference of light / heat / pH fluctuation and complex food matrix components, poor compatibility / dispersibility of a proteoglycan nano-carrier and a film-forming matrix, uneven color change caused by agglomeration, insufficient water resistance, biological activity, mechanical property and gas / water vapor barrier of a starch-based film and the like. And rapid, reversible and naked-eye-readable visual response can be realized in a pH / TVBN interval related to aquatic product spoilage.

The application discloses a triptolide nano-micelle, a synthesis method and application thereof. The nano-micelle is formed by self-assembly of triptolide, metal ions and a modular functional polymer through metal coordination; and the high molecular polymer is covalently connected by a metal coordination group R1, a hydrophilic polymer main chain R2 and a functional end-capping group R3. The application constructs a micelle system by relying on the coordination of three components without an exogenous carrier, avoids potential biological safety risks accompanied by traditional nano-carriers, reduces the complexity of the synthesis process of the medicament, and significantly improves the storage stability of the preparation after freeze-drying. The obtained micelle has good water solubility, can be quickly reconstituted after freeze-drying without a freeze-drying protective agent, has an ATP response drug release characteristic in a tumor microenvironment, and can be used for antitumor drug delivery.

Provided are a nitrogen-doped cellulose-based carbon nano-carrier material, a preparation method therefor, and the use thereof. The preparation method comprises the following steps: (1) pretreating a nanocellulose sol to obtain a uniformly distributed nanocellulose solution; (2) freezing the nanocellulose solution and vacuum drying same to obtain a nanocellulose aerogel; (3) pre-carbonizing the nanocellulose aerogel in an inert atmosphere, and cooling same to obtain a nanocellulose carbon aerogel; and (4) placing the nanocellulose carbon aerogel in urea powder, and performing high-temperature vapor deposition in an inert atmosphere to obtain the nitrogen-doped nanocellulose-based carbon nano-carrier material. The selected raw materials of the carbon nano-carrier material in the method are plant fibers, and have strong mechanical strength, wide sources and low costs. The prepared nitrogen-doped cellulose-based carbon nano-carrier material has good surface wettability, excellent electrical conductivity and stable properties.

The invention provides a cardiac targeting nano drug delivery carrier based on recombinant apolipoprotein, a system, a preparation method and application. The cardiac targeting nano drug delivery carrier comprises a nano carrier core and a functional layer covalently coupled to the surface of the nano carrier core, the nano carrier core comprises an amphiphilic copolymer formed by polylactic acid-glycolic acid and polyethylene glycol; the functional layer comprises recombinant apolipoprotein A-I and heart targeting peptide; the recombinant apolipoprotein A-I contains mutation sites E191A and F225W, and the recombinant apolipoprotein A-I contains mutation sites F225W; the amino acid sequence of the recombinant apolipoprotein A-I is as shown in SEQ ID NO: 1. The cardiac targeting nano drug delivery system based on the recombinant apolipoprotein has the functions of long circulation, cardiac targeting and coordinated regulation and control of metabolism and inflammation, and can be used for precise treatment of chronic heart failure.

The invention belongs to the technical field of drug carriers, and particularly relates to a magnetic nano-drug carrier which is prepared by the following steps: selecting traditional Chinese medicine raw materials and auxiliary raw materials, and selecting corresponding equipment for treating the raw materials; traditional Chinese medicine raw materials are pretreated, effective components are extracted through ultrasonic and reflux combination, and finally an extracting solution is purified and concentrated; preparing through a coprecipitation method; the flexible traditional Chinese medicine dressing is prepared by taking anti-inflammatory, analgesic, blood-activating and blood-stasis-removing active ingredients of 19 traditional Chinese medicines as cores and combining with the local magnetic response enrichment characteristic of the magnetic nano-carrier, the flexible traditional Chinese medicine dressing is attached to a pressurized silica gel pad of a PCI postoperative wrist strap for use, local directional enrichment of the active ingredients of the traditional Chinese medicines in a wound is promoted through the magnetic carrier, and the wound healing effect is improved. The pressurizing silica gel pad has the synergistic effects of resisting inflammation, easing pain, improving microcirculation and dredging collaterals and restoring the pulse, solves clinical pain points such as inflammation edema, obvious pain and unsmooth local blood supply of a wrist puncture wound after the PCI operation, and meets the requirements of flexibility, ventilation and fitting of the pressurizing silica gel pad at the same time.

The present application relates to the field of pharmaceutical preparation and nanomedicine, and particularly relates to a kind of nano-drug composition, its combined drug delivery system and application.The nano-drug composition comprises alpha 1-AR blocker and amphiphilic nano-carrier;The alpha 1-AR blocker is loaded in the hydrophobic core formed by amphiphilic nano-carrier;The alpha 1-AR blocker is selected from one or more of prazosin, terazosin, doxazosin;The average particle size of the nano-drug composition is 220-260 nm, the polydispersity index is less than 0.3, and the encapsulation efficiency is greater than 50%.The nano-drug composition of the present application has dual functions of anti-tumor proliferation and immune microenvironment remodeling, improves the dispersion stability of the drug in physiological medium, effectively reduces the in vivo clearance rate, prolongs the circulation time, and realizes the efficient in vivo delivery of the drug.The preparation process of the nano-preparation is stable and can be produced on a large scale.

The invention discloses iron-based nano laccase and a preparation method and decoloration application thereof, and relates to the technical field of iron-based nano laccase preparation, and the preparation method comprises the following steps: S1, iron-based nano carrier pretreatment; s2, synergistically modifying the iron-based nano-carrier; s3, preparing a laccase buffer solution; s4, carrying out ultrasonic and microwave synergistic in-situ loading; s5, step-by-step curing is carried out; s6, gradient purification; and S7, vacuum drying. According to the method, the binding effect of the laccase and the carrier is greatly improved, the problems of low laccase loading rate and large enzyme activity loss in the prior art are effectively improved, the product has excellent catalytic activity and stability, meanwhile, the binding firmness of the laccase and the carrier is further enhanced through the step-by-step curing process, laccase falling in the using process is reduced, and the service life of the product is prolonged. And in combination with excellent magnetic responsiveness of the iron-based nano-carrier, rapid separation, recovery and reutilization of the product are realized, and the practical application cost is greatly reduced.

The invention discloses a silverweed cinquefoil root flowering fertilizer composite formula and application thereof, and relates to the technical field of compound fertilizers, the silverweed cinquefoil root flowering fertilizer composite formula comprises, by weight, 15-25 parts of a nano carrier, 28-44 parts of an organic raw material, 7-15 parts of an inorganic raw material, 8-12 parts of biological bacteria, 0.2-0.3 part of a biological regulator, and 6-11 parts of a trace element composition; wherein the nano carrier is prepared from 12 to 20 parts of nano diatomite and 3 to 5 parts of nano hydroxyapatite; the nano diatomite is obtained by sequentially stirring a 2mol / L NaOH solution at the temperature of 60 DEG C for 2 hours, adjusting the pH value to 6.0 by a 1mol / L HCl solution and drying at the temperature of 105 DEG C; the specific surface area of the nano diatomite is greater than or equal to 85 m < 2 > / g The application has the effects of meeting the nutrient and physiological requirements in the key flowering period of the potentilla anserine and improving the flowering rate and the fruit setting rate of the potentilla anserine through nano-carrier modification and functional component optimization.

The invention discloses a neutralizing antibody with an AQP3 channel blocking function as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The neutralizing antibody can be specifically combined with an extracellular domain of AQP3, after combination, a water channel and a small molecule transport function of the AQP3 are blocked through steric hindrance and conformation change, and meanwhile, antigen combination specificity is reserved. The preparation method is realized through'recombinant antigen-immunization-B cell screening culture-recombinant antibody expression and affinity purification-ELISA detection ', and the functions of the antibody are ensured. The antibody can be efficiently combined with AQP3 positive cells, is strong in targeting property and high in specificity (the serum titer reaches 1: 1024 * 10, and the purified antibody titer is greater than or equal to 1: 128 * 10), and can be independently used or combined with a nano-carrier for treating related diseases, so that the technical bottleneck that a common AQP3 antibody can only be combined and cannot efficiently play a blocking role is solved.

The invention provides a polypeptide for enhancing T cell function and application thereof, and belongs to the technical field of biological medicine. The amino acid sequence of the polypeptide disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, an interaction interface of CLDN18.2 and beta-catenin is targeted for the first time, the full D-type functional polypeptide is designed to realize high-stability and high-affinity protein-protein interaction blocking, and the problem that the interaction site is difficult to target by the traditional antibody drug or small molecule drug is broken through; the used polypeptide PC18.1 has good in-vitro stability and is suitable for subsequent clinical development; the polypeptide is easy to prepare on a large scale through a synthesis means, has the purity of more than or equal to 98%, is low in cost and convenient to modify, and can be flexibly applied to immune combined treatment schemes such as nano-carrier loading and local delivery.

The invention discloses a biocontrol bacterium agent and a preparation method and application thereof.The biocontrol bacterium agent comprises a nano carrier and biocontrol bacteria, the carrier is halloysite nanotubes modified by coating the surfaces of the halloysite nanotubes with lecithin, sucrose ester or sophorolipid, and the biocontrol bacteria are bacillus subtilis; the preparation method comprises the following steps: (1) soaking halloysite nanotubes in a hydrochloric acid solution for pretreatment; (2) adding halloysite, lecithin, sucrose ester or sophorolipid into a solvent, uniformly mixing, reacting, and after the reaction is completed, washing and drying to obtain a modified halloysite nanotube; and (3) mixing a bacillus subtilis liquid with the modified halloysite nanotube, incubating, and drying to obtain the halloysite nanotube. According to the invention, lecithin, sucrose ester or sophorolipid is adopted to modify the halloysite nanotube and then is used as a carrier, so that the loading effect on bacillus subtilis is improved, and the fungicide has excellent prevention and treatment effects on tobacco brown spot, black shank, anthracnose and sweet leaf fungus damping-off.

The invention discloses chloroindole hydrazide-loaded nanogel and a preparation method and application thereof, and relates to the technical field of pesticides, the chloroindole hydrazide-loaded nanogel is successfully prepared, CHI can be stably loaded, slow release is realized, and the chloroindole hydrazide-loaded nanogel has excellent leaf adhesiveness, can resist rain wash and avoid loss of pesticide effect, and can be used as a pesticide for preventing and treating diseases. The application pain point of CHI and the defects of a traditional nano-carrier are overcome; the nano-scale characteristic assists plant cell endocytosis and uptake, breaks through the transport limitation of cell membranes, and gives full play to the high induced resistance activity of CHI. High-speed centrifugal purification is not needed in the preparation process, the process is simplified, the yield is increased, large-scale production is adapted, and meanwhile energy consumption and cost are reduced. Compared with a traditional nano-carrier, the nano-gel does not need expensive precursors or complex modification, is good in dispersity and free of the agglomeration problem, preparation is low in energy consumption and pollution, the comprehensive performance is more suitable for practical application of pesticide carriers, and a solution with practicability and economical efficiency is provided for efficient prevention and treatment of plant virus diseases.

The invention relates to the technical field of nano-carriers, and particularly discloses a preparation method and application of a nano-active millettia extract, the preparation method comprises the following steps: constructing a nano-carrier to wrap the millettia extract, and providing dual drug protection through a bimolecular layer lipid core and a chitosan shell, so that the nano-active millettia extract is obtained. The stability of the millettia bicolor extract in the nano-carrier is improved, and the requirements of the market for high efficiency and stability of a millettia bicolor extract nano-delivery system are met.

A nano-preparation for promoting tissue repair and use thereof. The nano-preparation comprises a circular RNA encoding a growth factor, a circular RNA encoding a cytokine, and a nano-vector. The nano-preparation can accurately express and release a growth factor and a cytokine in a cell, regulate the immune environment, inhibit inflammation, and promote wound tissue repair.

The invention belongs to the technical field of biological medicine, and provides a nano-carrier drug delivery system loaded with carboplatin as well as a preparation method and application of the nano-carrier drug delivery system. The preparation method comprises the following steps: (1) dissolving AZ-COOH, EDCI and HOBT in DMSO, and activating to obtain a first mixed solution; dSPE-PEG2000-NH2 and TEA are dissolved in DMSO (dimethyl sulfoxide), and a second mixed solution is obtained after stirring; dropwise adding the first mixed solution into the second mixed solution, reacting, dialyzing, and drying to obtain a nano-carrier DSPE-PEG-CAI; (2) dissolving the nano carrier DSPE-PEG-CAI and carboplatin in an organic solvent to obtain an organic phase; adding ultrapure water, continuously performing ultrasonic treatment, and volatilizing the organic solvent to obtain a water phase; and carrying out aqueous phase dialysis, and filtering to obtain a nano carboplatin DSPE-PEG-CAI-CBP aqueous solution. The carboplatin-loaded nano-carrier drug delivery system provided by the invention can improve the targeting property and intracellular enrichment degree of drugs, and passes through a biological barrier established by a tumor microenvironment, so that the targeting selectivity to triple negative breast cancer cells is enhanced, and the side effects of carboplatin treatment are reduced.

The invention discloses preparation of a drug-loaded aggregate derived nano-carrier and application of the drug-loaded aggregate derived nano-carrier in treatment of arthritis, and belongs to the technical field of nano-drug carriers. The condensate-derived nano-carrier provided by the invention is stable in a physiological environment, and the preparation method of the condensate-derived nano-carrier comprises the following steps: mixing polyphenol and an eight-arm polyethylene glycol solution to form a polyphenol-polyethylene glycol condensate, then adding a cross-linking agent, reacting, and dialyzing to obtain the condensate-derived nano-carrier. The aggregate-derived nanocarriers can be used as ROS scavengers and lubricants for ameliorating osteoarthritis; furthermore, after the polypeptide drug salmon calcitonin (sCT) is loaded, the effect of synergistically improving osteoarthritis can be achieved.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.