Quick detecting method of bovine leukemia virus
A detection method, virus technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of time-consuming and labor-intensive sensitivity, poor sensitivity, and serological detection methods that cannot meet the purification requirements, so as to facilitate collection and guarantee Effects of Accuracy and Stability
Inactive Publication Date: 2019-01-11
SHANDONG VOCATIONAL ANIMAL SCI & VETERINARY COLLEGE
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Benefits of technology
This patented technology allows us direct use of whole blood instead of specific parts like cells or plasma from cattle infected with BLV. It simplifies the process by eliminating any extra steps needed before testing it. By performing this analysis at least one section of the genome containing gene fragments that indicate how severe disease affects sheep's immune system, we aimed towards developing an improved way to accurately identify diseases such as lymphoma caused by Babesia bovis.
Problems solved by technology
Technics: Current diagnoses involving detecting bovidence disorders involve testing animals from different parts of their body through various techniques like histopathologic examination and culture analysis. However these tests require significant resources due to the lengthy duration involved and poor accuracy rates associated with current methodologies. Additionally, existing methods often lack specificity towards certain types of pathogens making them hard targets against other strains causing cross resistance issues during research efforts aiming at developing new therapies targeting those resistant forms of bacterial growth.
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[0041] With reference to accompanying drawing, bovine leukemia virus quick detection method of the present invention is characterized in that comprising the steps:
[0042] Step 1) Collect the peripheral blood of diseased cattle and latent poisonous cattle as blood samples;
[0043] Step 2) Extract DNA from the blood sample using a nucleic acid extraction kit;
[0044] Step 3) Perform nested PCR detection on blood sample DNA;
[0045] LTR1-1 / LTR1-2 with a fragment size of 399bp was used as the primer for the first PCR detection;
[0046] LTR2-1 / LTR2-2 with a fragment size of 279bp was used as the detection primer for the second PCR;
[0047] Wherein, the sequence of the detection primer LTR1-1 is 5'-CCCCGTAAACCAGACAGAGA-3';
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The invention relates to a quick detecting method of bovine leukemia virus, which directly takes blood as a detecting sample, does not need special treatment, and is convenient to collect, store and transport. Primers are designed to amplify LTR of bovine leukemia virus (BLV) provirus, which ensured the accuracy and stability of the method. Based on the nested PCR detecting method, the quick detecting method of bovine leukemia virus can detect bovine leukemia virus (BLV) in blood samples quickly, and its sensitivity is 1 fg DNA template, which is 100 times higher than that of PCR. The quick detecting method of bovine leukemia virus provides a quick and accurate diagnostic tool for the clinical diagnosis of bovine leukemia, and has important practical significance for the epidemiological investigation and purification of bovine leukemia.
Description
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