32 results about "Phorbol" patented technology

This invention concerns a process to convert a hydroxyl group (bold in R3C—OH) in a tigliane-type compound to a hydrogen (bold in R3C—H) to obtain deoxytigliane-type compounds or structural or functional analogs thereof. The process has wide application particularly to produce specific biologically active compounds in quantity for use as pharmaceuticals. In particular the process can be used to convert phorbol to a 12-deoxytigliane (prostrating which is a therapeutic lead for the treatment of AIDS. New compositions of matter are also disclosed.

Agents that induce platelet fragmentation include an IgG antibody that reacts with platelet epitope GPIIIA49-66 on platelet membrane, recombinant AMANTS-18, phorbol 12-myristate 13-acetate (PMA) and A23817.

The invention discloses a barbadosnut phorbol ester water emulsion insecticide. The barbadosnut phorbol ester water emulsion insecticide comprises the following components by weight: 1-20 percent of barbadosnut phorbol ester, 5-20 percent of a solvent, 3-20 percent of an emulsifier, 5-20 percent of a cosolvent, 2-5 percent of an antifreeze, 2-15 percent of a stabilizer, and the balance of water. The invention further discloses a preparation method of the barbadosnut phorbol ester water emulsion insecticide. Due to the fact that effective component of the product provided by the invention is phorbol ester extracted from barbadosnut seed oil and obtained as one of the side products generated producing a biodiesel, not only the barbadosnut resource is used comprehensively and the gap of using phorbol ester as the environment-friendly botanic pesticides is filled, but also the source of raw materials is wide, the cost is low, and the insecticide is easy to degrade and has no pollution to the environment. At the same time, as phorbol ester is a secondary metabolite of the plants, the product is featured with a wide insecticidal spectrum and various use ways, and is convenient to popularize.

The invention discloses a method for establishing a "human-derived" myocardial hypertrophy model based on pluripotent stem cell technology. The method is to resuspend the cardiomyocytes differentiated from pluripotent stem cells that have been cultured for about 30 days and then inoculate them into a 12-well plate with 1.5 × 10 cells per well. 6 cell. About 24 hours after the cells adhere to the wall, the phorbol-12-myristate-13-acetate dosing experiment can be started, and the induction is carried out at a concentration of 1 μM for 4 days. The invention can establish a stable human-derived myocardial hypertrophy model based on the pluripotent stem cell technology, and the shape and function conform to the characteristics of myocardial cell hypertrophy, the operation is convenient, and the modeling success rate is high.

A batch half-channel functional level detection method based on fluorescent dye uptake: (1) suspension culture, take THP-1 cells of a suspension culture cell line, use RPMI1640 culture medium supplemented with β-mercaptoethanol, and periodically add or replace the culture medium to protect cells The living environment is stable; monitor the concentration of the cell suspension does not exceed 1 × 10 6 cells / ml; the concentration of cell suspension was adjusted to 4×10 5 cells / ml, add tetradecanoyl phorbol acetate, inoculate the cell suspension in a 96-well plate, and change the medium after 24 hours to remove non-adherent cells; use blank RPMI1640 medium to maintain the culture to remove tetradecanoyl phorbol The effect of boitol acetate; (2) Fluorescent dye uptake to detect hemi-channel activity, ethidium bromide is a fluorescent dye, use standard RPMI1640 culture medium to prepare ethidium bromide fluorescent staining stock solution, and ethidium bromide staining stock solution The 96-well plate was added to the 96-well plate. After adding the fluorescent dye solution, the 96-well plate was placed on ice and incubated in the dark. The 96-well plate was placed in a fluorescence microplate reader to detect the fluorescence intensity.

The invention provides a cell membrane coated Au-Fe 3 o 4 The targeted nanomaterial and its preparation method and application belong to the technical field of targeted nanomaterials. Targeted nanomaterial cancer cell membrane provided by the present invention and Au-Fe wrapped in the cancer cell membrane 3 o 4 Nanoparticles, tannic acid and phorbol 12‑myristate 13‑acetate. PMA can effectively stimulate cells to produce H 2 o 2 and reactive oxygen species 2 ‑ , can suppress the insufficient H in cancer cells 2 o 2 Supplement to supplement raw materials for Fenton reaction; Fe 3 o 4 Can be partially decomposed in the presence of tannic acid to form Fe 2+ and Fe 3+ , Fe 2+ Can catalyze H in cancer cells 2 o 2 Decomposed into hydroxyl radicals, TA can promote Fe 3 o 4 Produced Fe 3+ to Fe 2+ Rapid transformation; at the same time, Au and Fe 3 o 4 It has good photothermal conversion efficiency and can give full play to the therapeutic effect of PTT.

The invention relates to the technical field of extraction of natural medicines, and specifically discloses a method for separating phorbol by ultrasonic-assisted alcoholysis-extraction coupling technology. The method includes the following steps: S1. According to the volume ratio of the material to the liquid, mix 1 part of croton oil with 5-25 parts of saturated alkaline alcohol solution evenly, carry out alcoholysis-extraction under the action of ultrasonic waves, stand and separate, and obtain phorbol-containing Alcohol alkaline supernatant and croton oil lower phase; S2. Adjust the obtained alkaline supernatant to neutral pH with an acidic alcohol solution, remove the precipitate, and obtain a neutral alcohol solution containing phorbol alcohol. The present invention utilizes ultrasonic-assisted alcoholysis-extraction coupling method to extract phorbol alcohol from croton oil, the method alcoholysis and extraction are carried out synchronously, and alcoholysis-extraction coupling is beneficial to improve the efficiency of alcoholysis, and with the assistance of ultrasonic waves, alcoholysis ‑Extraction is faster and more efficient, saving time and improving the purity of phorbol. The method has the advantages of few operation steps, short time consumption, small solvent consumption and easy popularization and application.

The invention relates to a method for eliminating alpha-synuclein generated in Parkinson's model cells by using cadmium telluride quantum dots and application thereof. The method comprises the following steps: spreading a cell suspension obtained by Parkinson's model cell digestion into 6 pore plates, wherein 3*10<4>-3*10<5> cells are spread in each hole; and after the cells are attached to walls, removing the culture medium in the hole, and adding a culture medium containing 4-75nM cadmium telluride quantum dots, thereby obviously eliminating the overexpressed alpha-synuclein in the Parkinson's model cells under the action of the cadmium telluride quantum dots. The Parkinson's model cells are SH-SY5Y cells which are constructed in vitro from 1-methyl-4-phenyl-pyridine ions and subjected to induced differentiation with all-trans-vitamin A acid and tetradecanoyl phorbol acetate, or differentiated PC12 cells constructed in vitro from 1-methyl-4-phenyl-pyridine ions. Cadmium telluride quantum dots with favorable biocompatibility are utilized to induce autophagy effect of cells, thereby eliminating overexpressed toxic protein (alpha-synuclein) in the Parkinson's model cell.

The present invention relates to a diterpene compound derived from Aleurites fordii, and a pharmaceutical composition for treating or preventing viral infectious diseases, a health functional food for preventing or ameliorating viral infectious diseases and a composition for enhancing the production of interferon-gamma, which comprise the diterpene compound. Furthermore, the present invention relates to a method for preventing or treating viral infectious diseases including administering the composition to a subject having a viral infectious disease occurrence or a risk thereof with a therapeutically effective dose.

The invention belongs to the technical field of the biological medicine, and relates to a microbial conversion method for a euphorbia lathyris diterpene compound. The method comprises the following steps: performing the microbial conversion to euphorbia lathyris phorbol and 7-hydroxy euphorbia lathyris phorbol by using Nocard's bacillus (i) Nocardia sp. ( / i) NRRL 5646, and preparing 3-carbonyl euphorbia lathyris phorbol and 7-acetoxyl euphorbia lathyris phorbol derivatives. Proved by the experiment, the polarity of the 3-carbonyl euphorbia lathyris phorbol in the derivative is reduced. Compared with a substrate, the derivative has the better cytotoxic activity. The derivative is an antitumor lead compound with development value.

A method for separating and culturing human primary melanocytes. According to the method, an ROCK inhibitor is added to a culture medium comprising a Ham's F-12 medium, dibutyryl cyclic adenosine monophosphate, 3-isobutyl-1-methylxanthine, sodium orthovanadate, phorbol 12-myristate 13-acetate, fetal bovine serum, and double antibodies.