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Targeting nanomaterial with cell membrane coated with Au-Fe3O4 as well as preparation method and application of targeting nanomaterial

An au-fe3o4 and nanomaterial technology, which is applied in the field of targeted nanomaterials coated with Au-Fe3O4 on cell membranes and their preparation, can solve problems such as hindering the efficacy of CDT therapy, normal cell influence, and normal human body function damage.

Active Publication Date: 2021-05-14
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, in tumor cells, H 2 o 2 The content of nano-drugs is small, which is not enough to produce enough hydroxyl radicals, which greatly hinders the curative effect of CDT therapy; at the same time, the introduction of non-tissue-specific nano-medicines will still have a greater impact on normal cells, and will have a negative impact on the normal cells of the human body. Functional damage

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  • Targeting nanomaterial with cell membrane coated with Au-Fe3O4 as well as preparation method and application of targeting nanomaterial
  • Targeting nanomaterial with cell membrane coated with Au-Fe3O4 as well as preparation method and application of targeting nanomaterial
  • Targeting nanomaterial with cell membrane coated with Au-Fe3O4 as well as preparation method and application of targeting nanomaterial

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preparation example Construction

[0040] The present invention provides the above-mentioned cell membrane coated Au-Fe 3 o 4 A method for preparing a targeted nanomaterial, comprising the following steps:

[0041] (1) Au-Fe 3 o 4 The nanoparticles, tannic acid, phorbol 12-myristate 13-acetate and solvent are shaken and mixed to obtain a mixed solution;

[0042] (2) Mix the cancer cell membrane with the mixed solution, and extrude the porous membrane to obtain the cell membrane-coated Au-Fe 3 o 4 targeted nanomaterials.

[0043] The present invention will Au-Fe 3 o 4 The nanoparticles, the tannic acid, the phorbol 12-myristate 13-acetate and the solvent are shaken and mixed to obtain a mixed solution. In the present invention, the Au-Fe 3 o 4 The preparation method of nanoparticles preferably comprises the following steps:

[0044] (a) heating and mixing gold nanoparticles, cetyl ammonium chloride, 1-octadecene, oleylamine and organic iron salts under anaerobic conditions to obtain Au-Fe nanoparticle...

Embodiment 1

[0078] (1) Synthesis of gold nanoparticles

[0079] (1) Under the gentle flow of nitrogen, 0.2g HAuCl 4 ·3H 2 O, 10mL 1,2,3,4-tetrahydronaphthalene and 10mL oleic acid amine were stirred and mixed until the liquid was orange-yellow; the above solution was cooled to 4°C in an ice bath;

[0080] (2) Dissolve 0.5 mmol of borane-tert-butylamine in a mixed solution of 1 mL of 1,2,3,4-tetrahydronaphthalene and 1 mL of oleylamine, dissolve it completely, and then pour it into the mixture obtained in step (1) solution, at this moment, the solution was dark purple, and the reaction continued for 2h;

[0081] (3) After the reaction is complete, add 50 mL of acetone, centrifuge at 10,000 r / min for 8 min, add 20 mL of n-hexane and 50 mL of ethanol to the resulting solid, and centrifuge at 9,000 r / min for 8 min to obtain gold nanoparticles, which are stored in n-hexane at 4°C in spare.

[0082] (2) Synthesis of Au-Fe nanoparticles

[0083] (1) Under argon flow, mix 1 mmol of HDAHCl so...

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Abstract

The invention provides a targeting nanomaterial with a cell membrane coated with Au-Fe3O4 as well as a preparation method and application of the targeting nanomaterial, belonging to the technical field of targeting nanomaterials. The targeting nanomaterial provided by the invention comprises a cancer cell membrane, Au-Fe3O4 nanoparticles, tannic acid and phorbol 12-myristate 13-acetate, wherein the Au-Fe3O4 nanoparticles, the tannic acid and the phorbol 12-myristate 13-acetate are wrapped in the cancer cell membrane. PMA can effectively stimulate cells to generate H2O2 and active oxygen O2 <->, can supplement insufficient H2O2 in cancer cells, and can supplement raw materials for Fenton reaction; the Fe3O4 can be partially decomposed in the presence of the tannic acid to form Fe<2+> and Fe<3+>, Fe<2+> can catalyze H2O2 in the cancer cells to be decomposed into hydroxyl radicals, and TA can promote rapid conversion of Fe<3+> generated by Fe3O4 into Fe<2+>; and meanwhile, Au and Fe3O4 have good photo-thermal conversion efficiency, and can give full play to the therapeutic effect of PTT.

Description

technical field [0001] The invention relates to the technical field of targeted nanomaterials, in particular to a cell membrane-coated Au-Fe 3 o 4 Targeted nanomaterials and their preparation methods and applications. Background technique [0002] For centuries, cancer has been one of the focal issues threatening human health, and many scientific researchers have worked hard to overcome this problem. So far, various treatment methods have been researched and developed, such as chemodynamic therapy (CDT therapy), photodynamic therapy (PDT therapy), photothermal therapy (PTT therapy), etc., among which, the combination of CDT therapy and PTT therapy has produced Good therapeutic effect. CDT treatment mainly utilizes metal and H 2 o 2 The highly active hydroxyl radicals (·OH) produced by the Fenton reaction between them can kill tumor cells. Nano-catalyzed drugs currently available for CDT therapy mainly contain Fe 2+ 、Cu 2 + , Mn 2+ and Co 2+ and other metal ions. ...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/46A61K41/00A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K31/7024A61K33/242A61K33/26
CPCA61K41/0052A61K9/5068A61K41/00A61K9/0009B82Y5/00B82Y30/00B82Y40/00A61P35/00A61K33/242A61K33/26A61K31/7024A61K31/23A61K2300/00
Inventor 郭英姝曹秀萍
Owner 山东济清科技服务有限公司
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