18 results about "Stimulator cell" patented technology

Contemplated treatments and methods produce substantially increased quantities of memory T-cells and a persistent immune response by subcutaneous and / or subdermal co-administration of (1) a vector comprising a recombinant nucleic acid that encodes a cancer associated epitope, a cancer specific epitope, and / or a neoepitope, (2) an immune stimulating cytokine, and (3) a checkpoint inhibitor. Most typically, the co-administration is performed at substantially the same location, preferably within 1-21 days from each other, and the vector is an adenoviral expression vector, for example, included in a viral particle such as an AdV5 virus with a deletion of the E2b gene.

The utility model discloses an angle-adjustable rotary tank type mesenchymal stem cell balling device, which relates to the technical field of mesenchymal stem cell balling, is arranged in a temperature control incubator for culturing mesenchymal stem cell balling, and comprises a bottle body for containing mesenchymal stem cells and a container for containing ultrasonic buffer water, the container is fixedly arranged on a horizontal bearing surface in the temperature control incubator, the bottle body is obliquely arranged, the bottom of the bottle body is positioned in the ultrasonic buffer water liquid of the container, the bottle body is driven to move by a driving device fixed on the horizontal bearing surface, and the driving device is configured to enable the bottle body to vibrate back and forth in the container along the horizontal direction and simultaneously drive the bottle body to move back and forth. And the bottle body also rotates around the axial direction of the bottle body. According to the utility model, the culture bottle simultaneously performs compound motion of horizontal oscillation and self rotation, so that the cell growth can be more uniformly stimulated, and the problem of poor balling effect caused by single rotation in the traditional method is solved. Through the movement mode, the cells are distributed more uniformly in the culture solution, and the formed spheres are more consistent in size and higher in quality.

Provided herein are methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography, and collecting the cells without using additional steps or reagents to facilitate detachment of the cells from the column. In some aspects, the methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering, and ultimately, cell therapy, compared to existing methods. Also provided are articles of manufacture and apparatus thereof.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The utility model relates to an electric stimulation cell culture device which comprises, from top to bottom, a cover, a fence, a conductive glass plate and a fixing frame. The fence is open at the top and bottom and closed around. A pressing mechanism is arranged between the fence and the cover or the fixing frame. The pressing mechanism is used to fix the fence on the fixing frame. The conductive glass plate is arranged between the fence and the fixing frame. The bottom of the fence is in sealing contact with the conductive glass plate to form a cell culture chamber. The length of the conductive glass plate is longer than that of the fence at both ends to form an electrode connecting part for connecting with an electrode. The electric stimulation cell culture device provided by the utility model avoids direct contact between the electrode and the culture medium, effectively avoids the risk of cell contamination, and has a simple overall structure, which can simplify experimental operation and ensure normal cell culture environment while applying electric stimulation.

Compositions and methods for stimulating the proliferation and cytotoxicity of γδ T cells are described. Therapeutic compositions and methods using the expanded and stimulated γδ T cells are described.

The application provides a kind of gamma delta T cell culture method and culture medium, belong to T cell culture technical field.In 1640 culture solution is added modified nanoparticle, zoledronic acid, vitamin C, glutamine, wherein, the concentration of the modified nanoparticle is 0.5-1g / L, the concentration of the zoledronic acid is 0.5-1.5 μmol / L, the concentration of the vitamin C is 200-500 μmol / L, the concentration of the glutamine is 0.1-0.2 mmol / L.The culture medium prepared by the application can efficiently stimulate gamma delta T cell expansion and improve its killing activity, and the survival rate of gamma delta T cells is also improved, and the culture method of gamma delta T cells is simple, the cost is low, and has broad application prospect.

The invention relates to the technical field of medicines, in particular to a zinc ion polypeptide compound immunologic adjuvant as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing polypeptide, a PBS buffer solution, new coronavirus S protein, a CpG ODN immune activator and zinc ions, and standing to obtain the zinc ion polypeptide compound immunologic adjuvant. A dual-adjuvant system mixed with CpG ODN shows excellent performance in activation of lung tissue immunity, and in the cellular immunity level, the response intensity of CD4 + T and CD8 + T cells is enhanced, and formation of related tissue resident memory T cell phenotypes is promoted; on the humoral immunity level, a Gel (Zn < 2 + >) and CpG ODN dual-adjuvant system obviously stimulates the increase of the number of B cells and the activation, and can effectively stimulate the secretion of a main antibody sIgA for mucosal immunity. On the whole, the dual adjuvants realize the synergistic enhancement of T cell (containing TRM) and B cell immunity.

The invention discloses a tuyere and a manufacturing method thereof.The tuyere is prepared from a high-temperature-resistant polymer base material, biological magnetic powder, graphene powder, metal powder, mineral substance powder and an auxiliary additive, and the manufacturing method comprises the following steps that S1, pretreatment is conducted; step S2, drying treatment; step S3, primary mixing; step S4, performing secondary mixing; s5, injection molding is carried out; s6, cooling, part taking and assembling are conducted; the manufactured tuyere is assembled on a hair dryer and connected with the hair dryer in a magnetic adsorption mode, after the hair dryer works and hot air passes through the tuyere, multi-band far infrared radiation of mineral materials and far infrared radiation of graphene form a superimposed effect, and heat energy and multi-broadband bands can be more effectively transmitted to a human body; in addition, graphene can form a weak biological electric field on the surface of the human body to stimulate cell activity, and negative ions released by mineral substances are in contact with the skin of the human body to regulate the physiological function of the human body.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

The invention relates to a three-specificity T cell adapter targeting HER2 (human epidermal growth factor receptor 2), HER3 (human epidermal growth factor receptor 3) and CD3 (human epidermal growth factor receptor 3) as well as a preparation method and application thereof. The three-specificity T cell adapter comprises an HER2 antigen binding structural domain, an HER3 antigen binding structural domain and a CD3 antigen binding structural domain which respectively target a human HER2 protein, a human HER3 protein and a human CD3 protein; the HER3 binding structural domain and the HER2 binding structural domain can target tumor cells expressing the two antigens, the CD3 binding structural domain can target T cells, the three-specificity T cell adapter can shorten the distance between the tumor cells and the T cells, and the T cells are stimulated to activate, so that the T cells have cytotoxic effect on the tumor cells; by combining the anti-HER3 binding structural domain and the anti-HER2 binding structural domain, on one hand, the action range and the action effect of the three-specificity T cell adapter can be increased, and on the other hand, the purposes of increasing specificity and reducing non-specific toxic and side effects can be achieved by targeting two different antigens of the same tumor cell at the same time.

The invention provides an electrically-driven magnetic rotary stirring device. The electrically-driven magnetic rotary stirring device comprises a magneton rotating structure, a fixed plate, a driving motor and a magnet, the magneton rotating structure is fixed on the fixed plate, the magnet is fixed on a rotating shaft of the driving motor, the driving motor drives the magnet to rotate through the rotating shaft so as to generate a rotating magnetic field, the magneton rotating structure is provided with a rotatable magneton, the magneton is located in the rotating magnetic field generated by the magnet, and the magneton is driven by the rotating magnetic field of the magnet to rotate. According to the device, the direct-current motor drives the magnet to generate a rotating magnetic field, the magneton is arranged in the rotating magnetic field generated by the magnet, meanwhile, the magneton is soaked in the cell culture fluid, and the rotating magnetic field generated by the magnet drives the magneton to rotate to stir the cell culture fluid so as to form the effect of a fluid shear force microenvironment; the cell culture medium can be well adapted to and applied to cell culture plates, including but not limited to 48-pore plates, 24-pore plates, 12-pore plates, 6-pore plates and the like.

A body-worn therapeutic speaker device is provided. The device is a body-worn therapeutic speaker designed to deliver vibrational sound therapy and music through wireless-enabled speaker units placed on various areas of the body. The device comprises at least one wireless speaker encased in a deformable, skin-safe material that conforms to different body regions for secure and effective placement. The speaker transmits specific vibrational frequencies, music, and sounds to promote circulation, muscle relaxation, pain relief, and emotional well-being by stimulating cellular activity and regulating neurotransmitter release. The device also includes an electromagnetic prevention mechanism to minimize radiation exposure, a rechargeable or disposable battery for power, and a mobile application for remote control of music, volume, and frequency selection.

The application discloses a bispecific antibody targeting PD-L1 and 4-1BB. The bispecific antibody contains a PD-L1 antigen binding domain (HCDR1, HCDR2 and HCDR3 are sequentially located at positions 26-32, 52-56 and 98-107 of SEQ ID No. 1, and LCDR1, LCDR2 and LCDR3 are sequentially located at positions 24-36, 52-58 and 93-100 of SEQ ID No. 2) and a 4-1BB antigen binding domain (HCDR1, HCDR2 and HCDR3 are sequentially located at positions 31-35, 50-65 and 98-106 of SEQ ID No. 3, and LCDR1, LCDR2 and LCDR3 are sequentially located at positions 24-34, 50-56 and 89-97 of SEQ ID No. 4). The bispecific antibody has good stability and high safety, can block the combination of PD-1 and PD-L1, activate the human 4-1BB signal path, stimulate T cell activation, and can be applied to an immune enhancer or an immune regulator of T cell-mediated autoimmune diseases.

The disclosure relates to methods and compositions for stimulating immune cells used in cell therapy with a bispecific bridging molecule that can be used to treat a disease or disorder, for example, cancer.

The invention relates to the technical field of biological extraction, in particular to an exosome preparation system which comprises a first storage tank, a second storage tank, a hollow fiber cell culture device, a tangential flow filtering device, a first peristaltic pump and a second peristaltic pump. An outlet of the second storage tank is connected with an inlet of the tangential flow filtering device through a second peristaltic pump, the ultrasonic generator and the ultrasonic transducer are additionally arranged in the air bag, and ultrasonic oscillation can stimulate cells and promote the cells to release more exosomes; a culture solution in a culture barrel is kept in a flowing state through a first peristaltic pump, nutrient elements in the culture solution can be supplemented, meanwhile, when an ultrasonic transducer drives a hollow vibration pipe to vibrate, the vibration frequencies of the two ends of the hollow vibration pipe are different, and the first peristaltic pump drives the culture solution to flow; the cells can receive oscillation stimulation of different frequencies, so that more exosomes are released, and large-scale industrial production of the exosomes is realized.