37 results about "Stimulator cell" patented technology

Contemplated treatments and methods produce substantially increased quantities of memory T-cells and a persistent immune response by subcutaneous and / or subdermal co-administration of (1) a vector comprising a recombinant nucleic acid that encodes a cancer associated epitope, a cancer specific epitope, and / or a neoepitope, (2) an immune stimulating cytokine, and (3) a checkpoint inhibitor. Most typically, the co-administration is performed at substantially the same location, preferably within 1-21 days from each other, and the vector is an adenoviral expression vector, for example, included in a viral particle such as an AdV5 virus with a deletion of the E2b gene.

The utility model discloses an angle-adjustable rotary tank type mesenchymal stem cell balling device, which relates to the technical field of mesenchymal stem cell balling, is arranged in a temperature control incubator for culturing mesenchymal stem cell balling, and comprises a bottle body for containing mesenchymal stem cells and a container for containing ultrasonic buffer water, the container is fixedly arranged on a horizontal bearing surface in the temperature control incubator, the bottle body is obliquely arranged, the bottom of the bottle body is positioned in the ultrasonic buffer water liquid of the container, the bottle body is driven to move by a driving device fixed on the horizontal bearing surface, and the driving device is configured to enable the bottle body to vibrate back and forth in the container along the horizontal direction and simultaneously drive the bottle body to move back and forth. And the bottle body also rotates around the axial direction of the bottle body. According to the utility model, the culture bottle simultaneously performs compound motion of horizontal oscillation and self rotation, so that the cell growth can be more uniformly stimulated, and the problem of poor balling effect caused by single rotation in the traditional method is solved. Through the movement mode, the cells are distributed more uniformly in the culture solution, and the formed spheres are more consistent in size and higher in quality.

[Problem] To provide a method for producing a dendritic cell formulation that stimulates NKT cells, from monocytes collected from a recipient (monocytes collected by apheresis or peripheral blood containing monocytes), and that can exhibit the effect more quickly than conventional products. [Solution] This method for producing a dendritic cell formulation comprises: an adhesion step for placing collected monocytes in a culture vessel using a liquid culture medium, and leaving the culture vessel still to cause a portion of the monocytes to adhere to the inner surface of the vessel; a non-adhering cell removal step for removing non-adhering cells including monocytes other than the cells adhering to the inner surface of the culture vessel; a differentiation step for adding a predetermined factor in the culture vessel to cause the monocytes adhering to the inner surface of the culture vessel to differentiate into immature dendritic cells; a pulsing step for performing, with α-galactosylceramide, pulsing in the culture vessel in which the immature dendritic cells are present in a non-adhering state; and an NKT-stimulating dendritic cell induction step for inducing, from the immature dendritic cells, NKT-stimulating dendritic cells that stimulate NKT cells. The dendritic cell formulation is administered back into a recipient from which the blood have been collected.

Provided herein are methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography, and collecting the cells without using additional steps or reagents to facilitate detachment of the cells from the column. In some aspects, the methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering, and ultimately, cell therapy, compared to existing methods. Also provided are articles of manufacture and apparatus thereof.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The utility model relates to an electric stimulation cell culture device which comprises, from top to bottom, a cover, a fence, a conductive glass plate and a fixing frame. The fence is open at the top and bottom and closed around. A pressing mechanism is arranged between the fence and the cover or the fixing frame. The pressing mechanism is used to fix the fence on the fixing frame. The conductive glass plate is arranged between the fence and the fixing frame. The bottom of the fence is in sealing contact with the conductive glass plate to form a cell culture chamber. The length of the conductive glass plate is longer than that of the fence at both ends to form an electrode connecting part for connecting with an electrode. The electric stimulation cell culture device provided by the utility model avoids direct contact between the electrode and the culture medium, effectively avoids the risk of cell contamination, and has a simple overall structure, which can simplify experimental operation and ensure normal cell culture environment while applying electric stimulation.

Compositions and methods for stimulating the proliferation and cytotoxicity of γδ T cells are described. Therapeutic compositions and methods using the expanded and stimulated γδ T cells are described.

The present invention relates to the identification and use of tumor epitopes from subjects with brain cancer, and particularly to epitopes from brevican, neurocan, versican, and aggrecan and their use in formulating cancer vaccines for treatment of tumor patients. In some preferred embodiments the methods provide a means of identifying T cell epitopes in proteins upregulated in brain tumors and the selection of those peptides which can stimulate T cell responses in individual subjects with a particular combination of HLA alleles. It further identifies the T cell exposed motifs comprised in T cell epitopes and enables the design of peptides with alternative amino acids in positions other than the T cell exposed motifs. In preferred embodiments, the MHC I and MHC II alleles of the affected subject are determined, and peptides are selected which bind to their MHC molecules with a desired affinity to elicit stimulation.

The application provides a kind of gamma delta T cell culture method and culture medium, belong to T cell culture technical field.In 1640 culture solution is added modified nanoparticle, zoledronic acid, vitamin C, glutamine, wherein, the concentration of the modified nanoparticle is 0.5-1g / L, the concentration of the zoledronic acid is 0.5-1.5 μmol / L, the concentration of the vitamin C is 200-500 μmol / L, the concentration of the glutamine is 0.1-0.2 mmol / L.The culture medium prepared by the application can efficiently stimulate gamma delta T cell expansion and improve its killing activity, and the survival rate of gamma delta T cells is also improved, and the culture method of gamma delta T cells is simple, the cost is low, and has broad application prospect.

B cell proliferation and / or memory B cell formation / expansion can be enhanced by contacting MAIT cells with nogapendekin alfa invaxcept (N-803) to produce stimulated MAIT cells, which stimulate B cell proliferation and / or memory B cell formation / expansion. Such stimulation can be performed in the presence of antigen or antigen-presenting cells. In particularly contemplated embodiments, MAIT cell stimulation is performed in vitro to produce immunostimulatory compositions and vaccines, or in vivo to enhance immune responses in airway tissue. Most typically, in vivo immune stimulation is performed by inhalation or intranasal delivery of a composition comprising N-803 and, optionally, vaccine components.

The invention relates to the technical field of medicines, in particular to a zinc ion polypeptide compound immunologic adjuvant as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing polypeptide, a PBS buffer solution, new coronavirus S protein, a CpG ODN immune activator and zinc ions, and standing to obtain the zinc ion polypeptide compound immunologic adjuvant. A dual-adjuvant system mixed with CpG ODN shows excellent performance in activation of lung tissue immunity, and in the cellular immunity level, the response intensity of CD4 + T and CD8 + T cells is enhanced, and formation of related tissue resident memory T cell phenotypes is promoted; on the humoral immunity level, a Gel (Zn < 2 + >) and CpG ODN dual-adjuvant system obviously stimulates the increase of the number of B cells and the activation, and can effectively stimulate the secretion of a main antibody sIgA for mucosal immunity. On the whole, the dual adjuvants realize the synergistic enhancement of T cell (containing TRM) and B cell immunity.

To provide a method for producing a large number of therapeutic extracellular vesicles (EVs).SOLUTION: There is provided a method for producing a large number of therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules. The method comprises: laying donor cells on a surface of a chip having a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed thereon; adding various plasmids, other transfection vectors, and combinations thereof to a buffer on the chip; applying a pulsatile electric field across the cells laid on the chip surface and a plasmid / vector buffer solution below the chip surface to strongly stimulate the cells and thereby deliver the plasmids / vectors into the cells by non-endocytic transfection; and collecting the EVs secreted by the transfected cells.SELECTED DRAWING: Figure 1

The invention discloses a tuyere and a manufacturing method thereof.The tuyere is prepared from a high-temperature-resistant polymer base material, biological magnetic powder, graphene powder, metal powder, mineral substance powder and an auxiliary additive, and the manufacturing method comprises the following steps that S1, pretreatment is conducted; step S2, drying treatment; step S3, primary mixing; step S4, performing secondary mixing; s5, injection molding is carried out; s6, cooling, part taking and assembling are conducted; the manufactured tuyere is assembled on a hair dryer and connected with the hair dryer in a magnetic adsorption mode, after the hair dryer works and hot air passes through the tuyere, multi-band far infrared radiation of mineral materials and far infrared radiation of graphene form a superimposed effect, and heat energy and multi-broadband bands can be more effectively transmitted to a human body; in addition, graphene can form a weak biological electric field on the surface of the human body to stimulate cell activity, and negative ions released by mineral substances are in contact with the skin of the human body to regulate the physiological function of the human body.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

Compositions and methods for stimulating NK cell expansion and cytotoxicity are described. Therapeutic compositions and methods using expanded and stimulated NK cells are described.

A proinflammatory cytokine production inhibitory apparatus for inhibiting production of a proinflammatory cytokine by magnetic stimulation of cells, the proinflammatory cytokine production inhibitory apparatus comprising:an electromagnetic wave production member to produce an electromagnetic wave at a proinflammatory cytokine production inhibitory frequency so as to expose the cells to an alternating magnetic field at the proinflammatory cytokine production inhibitory frequency;a frequency control member to control the proinflammatory cytokine production inhibitory frequency of the electromagnetic wave to 100 to 450 MHz so as to inhibit production of the proinflammatory cytokine by magnetic stimulation with the alternating magnetic field at the proinflammatory cytokine production inhibitory frequency; andan irradiation member to irradiate the cells with the alternating magnetic field at the proinflammatory cytokine production inhibitory frequency.

The invention relates to a three-specificity T cell adapter targeting HER2 (human epidermal growth factor receptor 2), HER3 (human epidermal growth factor receptor 3) and CD3 (human epidermal growth factor receptor 3) as well as a preparation method and application thereof. The three-specificity T cell adapter comprises an HER2 antigen binding structural domain, an HER3 antigen binding structural domain and a CD3 antigen binding structural domain which respectively target a human HER2 protein, a human HER3 protein and a human CD3 protein; the HER3 binding structural domain and the HER2 binding structural domain can target tumor cells expressing the two antigens, the CD3 binding structural domain can target T cells, the three-specificity T cell adapter can shorten the distance between the tumor cells and the T cells, and the T cells are stimulated to activate, so that the T cells have cytotoxic effect on the tumor cells; by combining the anti-HER3 binding structural domain and the anti-HER2 binding structural domain, on one hand, the action range and the action effect of the three-specificity T cell adapter can be increased, and on the other hand, the purposes of increasing specificity and reducing non-specific toxic and side effects can be achieved by targeting two different antigens of the same tumor cell at the same time.

The invention discloses a multifunctional friction micro-current textile and a preparation method thereof. According to a copper-containing conductive fiber layer, the copper content is larger than or equal to 2000 mg / kg, and a CuO nanocone array is arranged on the surface of the copper-containing conductive fiber layer; the graphene conductive fiber layer and the copper conductive fiber layer are woven in a staggered mode to form a friction interface, and the biological fiber layer accounts for 40-60% and provides skin-friendly performance and air permeability; the integrated micro-current generation system is used for generating 10-500 [mu] A pulse current through human body motion friction to stimulate cell repair and metabolism regulation (the integrated micro-current generation system is in accordance with a human body friction micro-current threshold value of IEC60601-1; the micro-current threshold value ranges from 0.5 mA to 10 mA, and accords with the IEC60479-1 safety contact current threshold value; the traditional Chinese medicine composition can stimulate cell repair, activate immune cells, improve microcirculation, inhibit lactic acid generation, inhibit inflammation and regulate metabolism.

The invention discloses a cell light activation system and activation method.The cell light activation system comprises a touch screen control module, an LED irradiation module, a power module and a data storage module, the touch screen control module provides a user interaction interface and is used for setting light activation parameters, and the LED irradiation module is electrically connected with the touch screen control module; the LED irradiation module generates light with a specific wavelength for cell light activation, the power supply module is electrically connected with the touch screen control module, the data storage module is electrically connected with the touch screen control module, and the data storage module is used for storing data in the light activation process; and the regeneration capacity of the cells can be obviously enhanced through a light activation technology. After tissue separation and homogenization, light activation can stimulate cells to secrete more growth factors and cell factors, and the factors are beneficial to own cells, can optimize the microenvironment of the whole tissue through paracrine action and can promote healing and regeneration of the tissue.

The invention provides an electrically-driven magnetic rotary stirring device. The electrically-driven magnetic rotary stirring device comprises a magneton rotating structure, a fixed plate, a driving motor and a magnet, the magneton rotating structure is fixed on the fixed plate, the magnet is fixed on a rotating shaft of the driving motor, the driving motor drives the magnet to rotate through the rotating shaft so as to generate a rotating magnetic field, the magneton rotating structure is provided with a rotatable magneton, the magneton is located in the rotating magnetic field generated by the magnet, and the magneton is driven by the rotating magnetic field of the magnet to rotate. According to the device, the direct-current motor drives the magnet to generate a rotating magnetic field, the magneton is arranged in the rotating magnetic field generated by the magnet, meanwhile, the magneton is soaked in the cell culture fluid, and the rotating magnetic field generated by the magnet drives the magneton to rotate to stir the cell culture fluid so as to form the effect of a fluid shear force microenvironment; the cell culture medium can be well adapted to and applied to cell culture plates, including but not limited to 48-pore plates, 24-pore plates, 12-pore plates, 6-pore plates and the like.

A body-worn therapeutic speaker device is provided. The device is a body-worn therapeutic speaker designed to deliver vibrational sound therapy and music through wireless-enabled speaker units placed on various areas of the body. The device comprises at least one wireless speaker encased in a deformable, skin-safe material that conforms to different body regions for secure and effective placement. The speaker transmits specific vibrational frequencies, music, and sounds to promote circulation, muscle relaxation, pain relief, and emotional well-being by stimulating cellular activity and regulating neurotransmitter release. The device also includes an electromagnetic prevention mechanism to minimize radiation exposure, a rechargeable or disposable battery for power, and a mobile application for remote control of music, volume, and frequency selection.

Embodiments of the invention provide apparatus and methods for stimulating L cells in the intestinal tract to produce incretins for the treatment of conditions including diabetes and obesity. Many embodiments provide a method and apparatus for the treatment of diabetes by electrically stimulating L-cells to secrete incretins to stimulate or otherwise modulate the production of insulin. Particular embodiments provide a swallowable capsule for stimulating L-cells in the intestinal tract as the capsule moves through the tract. The capsule can include two or more electrodes for providing electrical stimulation to L-cells, a power source for powering one or more components of the capsule, a sensor for sensing the location of the capsule in the intestinal tract; a controller and a waveform generator for generating the electrical signals emitted by the electrodes to stimulate the L-cells to secrete incretins such as GLP-1 to stimulate insulin production for glucose regulation of diabetic conditions.

The invention discloses a preparation method and application of carbohydrate protein CA72-4, and the preparation method comprises the following steps: inoculating adherent culture type LS-174T cells into a DMEM (Dulbecco Modified Eagle Medium) containing 5% and 2.5% of fetal calf serum for subculture; the low-serum adherent culture type LS-174T cell line is inoculated into a serum-free suspension culture medium to be subjected to subculture, and a serum-free full-suspension culture type LS-174T cell line is obtained; carrying out suspension culture on the LS-174T cell line, and adding a medicine in the suspension culture process to stimulate the LS-174T cell line to secrete CA72-4; and concentrating the cell supernatant which is cultured for 3-5 days, and changing the liquid to obtain concentrated CA72-4. The serum-free full-suspension culture type LS-174T cell line constructed by the invention can adapt to serum-free full-suspension culture, is beneficial to full contact between nutrient substances in a culture medium and cells and high-density growth of the cells, and reduces the cell culture difficulty in the production process; in addition, short-time large-scale production is facilitated, and the production cost is greatly reduced.

The application discloses a bispecific antibody targeting PD-L1 and 4-1BB. The bispecific antibody contains a PD-L1 antigen binding domain (HCDR1, HCDR2 and HCDR3 are sequentially located at positions 26-32, 52-56 and 98-107 of SEQ ID No. 1, and LCDR1, LCDR2 and LCDR3 are sequentially located at positions 24-36, 52-58 and 93-100 of SEQ ID No. 2) and a 4-1BB antigen binding domain (HCDR1, HCDR2 and HCDR3 are sequentially located at positions 31-35, 50-65 and 98-106 of SEQ ID No. 3, and LCDR1, LCDR2 and LCDR3 are sequentially located at positions 24-34, 50-56 and 89-97 of SEQ ID No. 4). The bispecific antibody has good stability and high safety, can block the combination of PD-1 and PD-L1, activate the human 4-1BB signal path, stimulate T cell activation, and can be applied to an immune enhancer or an immune regulator of T cell-mediated autoimmune diseases.

The present invention describes a whole blood assay chip that enables separation of the cellular compartment from surrounding plasma, facilitating multiplexed measurement of otherwise difficult to detect soluble factors produced upon cellular stimulation. The invention is a microfluidic chip with an incubation chamber comprising an inlet, an outlet, and a fluidic barrier. Using this platform, the cellular compartment can be stimulated with various substances and levels of immunologic biomarkers could be measured. Through a proprietary immunologic algorithm described herein, this would allow for the diagnosis of an allergic response to foods, antibiotics, and / or other drugs. Additional applications of the invention besides allergy testing could include pregnancy testing, blood type, and medical applications requiring whole blood cellular stimulation readouts.

The present invention is directed to the use of gold nanoparticles to enable vision in the visually impaired. Retinal neurons remain functional despite photoreceptor degeneration. These neurons can be made light-sensitive to produce action potentials, by gold nanoparticles conjugated to high-avidity ligands for a variety of cellular targets. Once bound to a neuron, these particles transduce millisecond pulses of light into heat, which changes membrane capacitance, depolarizing the cell and eliciting action potentials. The technology described herein along with a camera and decoder can be used to aid the visually impaired by stimulating cells to send the appropriate messages to the visual cortex.

Provided herein are devices and methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography. In some aspects. provided is a device comprising a temperature control member to provide heat to a chromatography stationary phase and a connector configured to provide air to the stationary phase during column chromatography. In some aspects. the devices and methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering. and ultimately, cell therapy. compared to existing devices and methods.