67 results about "Stimulator cell" patented technology

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and/or stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

The present invention relates to novel regulatory T cell proteins. One protein, designated PD-L3, resembles members of the PD-L1 family, and co-stimulates αCD3 proliferation of T cells in vitro. A second, TNF-like, protein has also been identified as being upregulated upon αCD3/αGITR stimulation. This protein has been designated T^reg-sTNF. Proteins, antibodies, activated T cells and methods for using the same are disclosed.

In particular methods of using these proteins and compounds, preferably antibodies, which bind or modulate (agonize or antagonize) the activity of these proteins, as immune modulators and for the treatment of cancer, autoimmune disease, allergy, infection and inflammatory conditions, e.g. multiple sclerosis is disclosed

The present invention provides methods and compositions for expanding Treg cells ex vivo or in vivo using one or more conjugates comprising a costimulatory moiety that stimulates at least one of three signals involved in Treg cell development and/or using dendritic cells pulsed with antigens and modified to display TGF-β, or hematopoetic stem cells or bone marrow cells modified to display TGF-β. The methods and compositions are useful, for example, in the treatment and prevention of autoimmune disease, including Type 1 diabetes and in preventing foreign graft rejection, as well as to establish mixed chimerism, induce tolerance to autoantigens, alloantigens or xenoantigens, beta cell regeneration, prevention of foreign graft rejection, and treatment of a genetically inherited hematopoietic disorder.

The present invention relates to methods and compositions for altering (e.g., augmenting or stimulating) differentiation and growth of cells (e.g., neural progenitor cells and neurons). In particular, the present invention relates to compositions comprising one or more self-assembling peptide amphiphiles (e.g., in solution or that generate (e.g., self-assemble into) nanofibers (e.g., that are able to encapsulate cells and promote cellular differentiation (e.g., neurite development))) and methods of using the same. Compositions and methods of the present invention find use in research, clinical (e.g., therapeutic) and diagnostic settings.

An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR α/β cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A. According to the present invention, a target TCR gene can be cloned within a shorter time compared with that repaired by the conventional methods, for example, about ten days. Further, according to the present invention, genes of TCR α chain and β chain can be highly efficiently cloned. Under the conditions of the examples, a pair of a TCR α chain and TCR β chain could be obtained from stimulated T cells sorted as single cells at a ratio of 100%.

The described invention provides a method for in vitro immunoactivation of mononuclear cells by contact with one or more populations of engineered leukocyte stimulator cells genetically engineered to express a core of 3 essential immunomodulator peptides, and optionally additional R immunomodulator peptides, and use of a cell product comprising the expanded and activated mononuclear cell population comprising one or more subpopulations of cytotoxic serial killer cells for passive immunization of a cancer patient not currently under the influence of an immunosuppressive regimen.

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

The invention discloses an artificial antigen submitting cell and preparing method, which comprises the following steps: choosing human chronic granular leukocyte leukemia bacterial strain K562 cell as carrier; transfer-dying and expressing eucaryon expressing carrier of CD32a; expressing CD32 molecular on the surface of K562 cell stably through G418 screening and flowed cell sorting device sorting; forming K32 cell; producing eucaryon expressing carrier of double expressing CD86 and 4-1BBL co-simulating signal; proceeding homomycin screen and sort with flowed cell device for the K32 cell; getting stable expression K32 cell of CD86 and 4-1BBL molecule; getting the end product. This invention possesses low cost and good repeatability, which can inhibit die effectively.

The invention aims at the special requirement of the memristor as the electronic component of the processor, presents a neuron and neuron circuit based on the memristor of brain-like device, which canrealize the signal storage and processing similar to human neuron cell, and its single neuron cell can be extensively connected with hundreds of memristors, which provides a very feasible circuit design way for the large-scale use of memristors. In combination with other types of electronic devices such as CMOS tubes, a selector, a nanowire, and pulse design, the invention creatively solves the polysynaptic connection in biology of neuron cell designed by memristor as the core device of processor, forward stimulation, backward stimulation, nuclear storage, synaptic front-end, protruding back-end and other biological design issues, to achieve (forward and backward) signal processing within neurons and transmission between neurons, and build the corresponding neuronal cells and neural network circuits.

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.

The invention provides an NK cell in-vitro amplification system. The NK cell in-vitro amplification system comprises an activation culture medium for cell activation and a proliferation culture medium for cell proliferation, the activating culture medium is composed of an activating agent and a basic culture medium, and the activating agent is a combination of solid-phase anti-CD52 and IL-2; the proliferation culture medium is composed of a proliferation agent and a basic culture medium, and the proliferation agent is IL-2. Compared with the prior art, the NK cell in-vitro amplification system disclosed by the invention has the following technical effects: 1) the culture medium contains the immobilized anti-CD52, and the NK cells can be efficiently stimulated to enter an activated and proliferated state by combining the use of IL-2, so that a large number of activated NK cells can be easily obtained; moreover, no trophoblasts are used in the culture process, and the prepared NK cells can be used for clinical research and treatment; and 2) the NK cell culture method is simplified, few NK cell activation components are used, few material resources and manpower are used, the production cost of NK cell preparation is obviously reduced, and the method is particularly suitable for large-scale production.

The present invention utilizes a hand piece featuring a laser device which is used in the irradiation of damaged cells to stimulate photoreceptors within a cell, initiating a cascade of intracellular metabolic reactions that move the cell toward homeostasis. As the cell approaches homeostasis there occur a number of secondary effects of photo stimulation which contribute to homeostatic cellular metabolism of adjacent cells. Concurrent photo stimulation of the maximal three dimensional volume of cells along with the secondary effects of the cellular metabolic homeostasis achieved in these cells results in a resolution of metabolic deficits within cells far in excess of the effects of direct photo stimulation. The current invention offers an effective method of irradiation of large numbers of cells with safe levels of optical energy to initiate the intracellular cascade toward homeostasis and the production of secondary effects contributing to homeostasis of adjacent or distant cells.

The invention provides a purification preparation method and an application of mesenchymal stem cell secreted factors. The purification preparation method comprises the following steps: (1) preparing MSC supernate; (2), purifying the mesenchymal stem cell secreted factors, including filtration and concentration, heparin affinity chromatography, and heparin affinity chromatography elution peak 5kD ultrafiltration or G-25 desalination column desalination; and (3) using the purified MSC secretion factors in the step (3) to prepare masks. According to the invention, a heparin affinity chromatography medium is adopted to specifically adsorb mesenchymal stem cell (MSC) secreted factors and purify substances with an effect of stimulating NIH 3T3 cell proliferation in MSC secretions, and the substances are potentially used for repairing refractory wounds and are added to cosmetics as an additive. The effects of repairing damaged skin, eliminating skin wrinkles, shrinking pores, improving complexion and the like can be achieved.

Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

The present invention discloses a pulse stirring bioreactor for secretion, separation and collection of exosomes. The pulse stirring bioreactor comprises a cell culture device and an exosome separation and collection device; the exosome separation and collection device is connected with the cell culture device and can collect the exosomes separated from a culture solution in the cell culture device; the cell culture device comprises a stirring device and is used for applying stirring power to the culture solution in the cell culture device; and the cell culture device comprises a pulse devicewhich can add the culture solution to the cell culture device in a pulsed manner. The bioreactor can enable the culture solution and cells to be fully mixed to keep enough oxygen supply, the stirringdevice is not in direct contact with the cells and reduces a shearing force of the cells, and at the same time, the pulse stirring bioreactor can simulate cell growth environment in vivo and stimulateNK cells or other suspended cells to secrete a large amount of cell products

Methods for enhancing beta cell survival and/or for stimulating beta cell proliferation, comprise co-transplanting pancreatic islets, beta cells, and/or stem cells which can generate beta cells, with (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem/progenitor cells (TetStock neural stem/progenitor cells), and/or (iii) pre-differentiated stem/progenitor neural cells. Methods for reinnervation in an organ or tissue transplant patient comprise co-transplanting with the organ or tissue (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem/progenitor cells (TetStock neural stem/progenitor cells), and/or (iii) pre-differentiated stem/progenitor neural cells. Kits for conducting such methods employ at least one of (i) neural crest stem cells (NCSCs), (ii) tetracycline-regulated gene expression system (Tet-System)-containing neural stem/progenitor cells (TetStock neural stem/progenitor cells), and (iii) pre-differentiated stem/progenitor neural cells.

The organ chip with the multi-layer structure comprises an upper flow channel layer, an upper porous membrane layer, a tissue layer, a lower porous membrane layer and a lower flow channel layer which are sequentially arranged in a stacked mode from top to bottom, and the upper flow channel layer comprises a first filling opening, a second filling opening, a first upper flow channel opening and a second upper flow channel opening; the hydrogel containing the cells is poured into the first pouring channel or the second pouring channel through the first pouring opening or the second pouring opening, and the hydrogel containing the cells fills the tissue cavity under the blocking of the upper porous membrane layer and the lower porous membrane layer; cell culture fluid is injected through the first upper flow channel opening and the second upper flow channel opening and permeates into the tissue cavity through the first porous membrane and the second porous membrane to culture cells. According to the organ chip, the perfusion channel of the hydrogel containing the cells can be separated from the perfusion channel of the cell culture fluid, the perfusion of the hydrogel containing the cells cannot block the perfusion channel of the cell culture fluid, and the cell culture fluid circulates in the organ chip to uniformly stimulate the cells.

The invention provides a targeting nanomaterial with a cell membrane coated with Au-Fe3O4 as well as a preparation method and application of the targeting nanomaterial, belonging to the technical field of targeting nanomaterials. The targeting nanomaterial provided by the invention comprises a cancer cell membrane, Au-Fe3O4 nanoparticles, tannic acid and phorbol 12-myristate 13-acetate, wherein the Au-Fe3O4 nanoparticles, the tannic acid and the phorbol 12-myristate 13-acetate are wrapped in the cancer cell membrane. PMA can effectively stimulate cells to generate H2O2 and active oxygen O2 <->, can supplement insufficient H2O2 in cancer cells, and can supplement raw materials for Fenton reaction; the Fe3O4 can be partially decomposed in the presence of the tannic acid to form Fe<2+> and Fe<3+>, Fe<2+> can catalyze H2O2 in the cancer cells to be decomposed into hydroxyl radicals, and TA can promote rapid conversion of Fe<3+> generated by Fe3O4 into Fe<2+>; and meanwhile, Au and Fe3O4 have good photo-thermal conversion efficiency, and can give full play to the therapeutic effect of PTT.

The present invention describes a whole blood assay chip that enables separation of the cellular compartment from surrounding plasma, facilitating multiplexed measurement of otherwise difficult to detect soluble factors produced upon cellular stimulation. The invention is a microfluidic chip with an incubation chamber comprising an inlet, an outlet, and a fluidic barrier. Using this platform, the cellular compartment can be stimulated with various substances and levels of immunologic biomarkers could be measured. Through a proprietary immunologic algorithm described herein, this would allow for the diagnosis of an allergic response to foods, antibiotics, and/or other drugs. Additional applications of the invention besides allergy testing could include pregnancy testing, blood type, and medical applications requiring whole blood cellular stimulation readouts.