52 results about "Stimulator cell" patented technology

The invention provides a construction method and application of a gene modified K562 cell line, and belongs to the technical field of biological medicines. According to the invention, two viruses CD86-4-1BBL and mbIL-21 are respectively subjected to gene transduction packaging by adopting a third-generation lentivirus four-plasmid packaging system, a K562 cell line is infected by the lentiviruses CD86-4-1BBL and mbIL-21, and a monoclonal cell line capable of stably expressing CD86, 4-1BBL and mbIL-21 proteins is obtained through screening. According to the method, cell lines with the cloning numbers of V5-E9, V10-A5, V5-D6 and V6-D7 are obtained through screening, the four cloning cell lines can serve as feeder cells for stimulating NK cell amplification after being amplified and subjected to irradiation treatment, NK cell amplification can be effectively promoted, the amplification multiple of the NK cells reaches up to 6000-8000, and large-scale preparation and production of the NK cells for treatment are met.

The invention relates to detection of vaccine immune effects, in particular to a Marek's disease virus specificity IFN-gamma sandwich ELISA method and application thereof. The invention provides a Marek's disease virus specific T cell immune stimulator, and the amino acid sequence of the Marek's disease virus specific T cell immune stimulator is shown as SEQ ID NO: 1 or SEQ ID NO: 3. The invention further provides a method for detecting the cellular immune level after Marek's disease vaccine immunization. The Marek's disease virus specific T cell immunostimulant can effectively stimulate cell-mediated immune response, initiate and activate MDV specific T cells, so that the Marek's disease virus specific T cells generate Marek's disease virus specific IFN-gamma, and the Marek's disease virus specific T cell immunostimulant has a good application prospect in Marek's disease vaccine immune level detection.

A system for treating various medical conditions in humans includes a medical or therapeutic laser operating at wavelengths in the near infrared range of between 1250 nm-1267 nm, with a target wavelength of 1260 nm, at power levels between 2 Watts and 60 Watts, and for a period not to exceed 60 seconds per dose. The emitted laser light energizes, e.g., the stem cell niche or mitochondria of the human tissue to which it is applied, both directly and indirectly via activation of cell signaling intermediaries including but not limited to heat shock proteins, thereby stimulating cell activity to promote healing and reduce pain. In this specific wavelength range, water absorption of the laser light energy is plateaued which effectively acts as a conduit allowing the laser light energy to be transferred deeper into the tissue without causing thermal damage to the skin and tissue.

Contemplated treatments and methods produce substantially increased quantities of memory T-cells and a persistent immune response by subcutaneous and / or subdermal co-administration of (1) a vector comprising a recombinant nucleic acid that encodes a cancer associated epitope, a cancer specific epitope, and / or a neoepitope, (2) an immune stimulating cytokine, and (3) a checkpoint inhibitor. Most typically, the co-administration is performed at substantially the same location, preferably within 1-21 days from each other, and the vector is an adenoviral expression vector, for example, included in a viral particle such as an AdV5 virus with a deletion of the E2b gene.

The utility model discloses an angle-adjustable rotary tank type mesenchymal stem cell balling device, which relates to the technical field of mesenchymal stem cell balling, is arranged in a temperature control incubator for culturing mesenchymal stem cell balling, and comprises a bottle body for containing mesenchymal stem cells and a container for containing ultrasonic buffer water, the container is fixedly arranged on a horizontal bearing surface in the temperature control incubator, the bottle body is obliquely arranged, the bottom of the bottle body is positioned in the ultrasonic buffer water liquid of the container, the bottle body is driven to move by a driving device fixed on the horizontal bearing surface, and the driving device is configured to enable the bottle body to vibrate back and forth in the container along the horizontal direction and simultaneously drive the bottle body to move back and forth. And the bottle body also rotates around the axial direction of the bottle body. According to the utility model, the culture bottle simultaneously performs compound motion of horizontal oscillation and self rotation, so that the cell growth can be more uniformly stimulated, and the problem of poor balling effect caused by single rotation in the traditional method is solved. Through the movement mode, the cells are distributed more uniformly in the culture solution, and the formed spheres are more consistent in size and higher in quality.

[Problem] To provide a method for producing a dendritic cell formulation that stimulates NKT cells, from monocytes collected from a recipient (monocytes collected by apheresis or peripheral blood containing monocytes), and that can exhibit the effect more quickly than conventional products. [Solution] This method for producing a dendritic cell formulation comprises: an adhesion step for placing collected monocytes in a culture vessel using a liquid culture medium, and leaving the culture vessel still to cause a portion of the monocytes to adhere to the inner surface of the vessel; a non-adhering cell removal step for removing non-adhering cells including monocytes other than the cells adhering to the inner surface of the culture vessel; a differentiation step for adding a predetermined factor in the culture vessel to cause the monocytes adhering to the inner surface of the culture vessel to differentiate into immature dendritic cells; a pulsing step for performing, with α-galactosylceramide, pulsing in the culture vessel in which the immature dendritic cells are present in a non-adhering state; and an NKT-stimulating dendritic cell induction step for inducing, from the immature dendritic cells, NKT-stimulating dendritic cells that stimulate NKT cells. The dendritic cell formulation is administered back into a recipient from which the blood have been collected.

Provided herein are methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography, and collecting the cells without using additional steps or reagents to facilitate detachment of the cells from the column. In some aspects, the methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering, and ultimately, cell therapy, compared to existing methods. Also provided are articles of manufacture and apparatus thereof.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The invention relates to the technical field of plate flow chambers, and discloses a cell co-culture plate flow chamber and a gradient fluid shear stress stimulation method thereof, the top of a base is provided with a flow chamber composition mechanism, and the right side of the flow chamber composition mechanism is provided with a pump circulation mechanism; the flowing cavity forming mechanism comprises a cavity shell, the cavity shell is fixedly installed at the top of the base, a silica gel pad is arranged on the inner side of the bottom of the cavity shell, a glass slide is arranged at the top of the silica gel pad, and an upper cover is installed in the cavity shell in a sliding mode. According to the cell co-culture plate flow cavity and the gradient fluid shear stress stimulation method thereof, in the use process, the pipeline which is not in contact with the peristaltic pump is utilized, so that pollution can be prevented, and stable conveying can be kept; the effect that the flat plate flow cavity stimulates cells through the flow force stress of fluid can be effectively improved, the polished organic glass material can assist a microscope in observation, and the cell co-culture effect is improved.

The utility model relates to an electric stimulation cell culture device which comprises, from top to bottom, a cover, a fence, a conductive glass plate and a fixing frame. The fence is open at the top and bottom and closed around. A pressing mechanism is arranged between the fence and the cover or the fixing frame. The pressing mechanism is used to fix the fence on the fixing frame. The conductive glass plate is arranged between the fence and the fixing frame. The bottom of the fence is in sealing contact with the conductive glass plate to form a cell culture chamber. The length of the conductive glass plate is longer than that of the fence at both ends to form an electrode connecting part for connecting with an electrode. The electric stimulation cell culture device provided by the utility model avoids direct contact between the electrode and the culture medium, effectively avoids the risk of cell contamination, and has a simple overall structure, which can simplify experimental operation and ensure normal cell culture environment while applying electric stimulation.

Compositions and methods for stimulating the proliferation and cytotoxicity of γδ T cells are described. Therapeutic compositions and methods using the expanded and stimulated γδ T cells are described.

The present invention relates to the identification and use of tumor epitopes from subjects with brain cancer, and particularly to epitopes from brevican, neurocan, versican, and aggrecan and their use in formulating cancer vaccines for treatment of tumor patients. In some preferred embodiments the methods provide a means of identifying T cell epitopes in proteins upregulated in brain tumors and the selection of those peptides which can stimulate T cell responses in individual subjects with a particular combination of HLA alleles. It further identifies the T cell exposed motifs comprised in T cell epitopes and enables the design of peptides with alternative amino acids in positions other than the T cell exposed motifs. In preferred embodiments, the MHC I and MHC II alleles of the affected subject are determined, and peptides are selected which bind to their MHC molecules with a desired affinity to elicit stimulation.

The application provides a kind of gamma delta T cell culture method and culture medium, belong to T cell culture technical field.In 1640 culture solution is added modified nanoparticle, zoledronic acid, vitamin C, glutamine, wherein, the concentration of the modified nanoparticle is 0.5-1g / L, the concentration of the zoledronic acid is 0.5-1.5 μmol / L, the concentration of the vitamin C is 200-500 μmol / L, the concentration of the glutamine is 0.1-0.2 mmol / L.The culture medium prepared by the application can efficiently stimulate gamma delta T cell expansion and improve its killing activity, and the survival rate of gamma delta T cells is also improved, and the culture method of gamma delta T cells is simple, the cost is low, and has broad application prospect.

B cell proliferation and / or memory B cell formation / expansion can be enhanced by contacting MAIT cells with nogapendekin alfa invaxcept (N-803) to produce stimulated MAIT cells, which stimulate B cell proliferation and / or memory B cell formation / expansion. Such stimulation can be performed in the presence of antigen or antigen-presenting cells. In particularly contemplated embodiments, MAIT cell stimulation is performed in vitro to produce immunostimulatory compositions and vaccines, or in vivo to enhance immune responses in airway tissue. Most typically, in vivo immune stimulation is performed by inhalation or intranasal delivery of a composition comprising N-803 and, optionally, vaccine components.

The invention relates to the technical field of medicines, in particular to a zinc ion polypeptide compound immunologic adjuvant as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing polypeptide, a PBS buffer solution, new coronavirus S protein, a CpG ODN immune activator and zinc ions, and standing to obtain the zinc ion polypeptide compound immunologic adjuvant. A dual-adjuvant system mixed with CpG ODN shows excellent performance in activation of lung tissue immunity, and in the cellular immunity level, the response intensity of CD4 + T and CD8 + T cells is enhanced, and formation of related tissue resident memory T cell phenotypes is promoted; on the humoral immunity level, a Gel (Zn < 2 + >) and CpG ODN dual-adjuvant system obviously stimulates the increase of the number of B cells and the activation, and can effectively stimulate the secretion of a main antibody sIgA for mucosal immunity. On the whole, the dual adjuvants realize the synergistic enhancement of T cell (containing TRM) and B cell immunity.

To provide a method for producing a large number of therapeutic extracellular vesicles (EVs).SOLUTION: There is provided a method for producing a large number of therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules. The method comprises: laying donor cells on a surface of a chip having a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed thereon; adding various plasmids, other transfection vectors, and combinations thereof to a buffer on the chip; applying a pulsatile electric field across the cells laid on the chip surface and a plasmid / vector buffer solution below the chip surface to strongly stimulate the cells and thereby deliver the plasmids / vectors into the cells by non-endocytic transfection; and collecting the EVs secreted by the transfected cells.SELECTED DRAWING: Figure 1

The invention discloses a tuyere and a manufacturing method thereof.The tuyere is prepared from a high-temperature-resistant polymer base material, biological magnetic powder, graphene powder, metal powder, mineral substance powder and an auxiliary additive, and the manufacturing method comprises the following steps that S1, pretreatment is conducted; step S2, drying treatment; step S3, primary mixing; step S4, performing secondary mixing; s5, injection molding is carried out; s6, cooling, part taking and assembling are conducted; the manufactured tuyere is assembled on a hair dryer and connected with the hair dryer in a magnetic adsorption mode, after the hair dryer works and hot air passes through the tuyere, multi-band far infrared radiation of mineral materials and far infrared radiation of graphene form a superimposed effect, and heat energy and multi-broadband bands can be more effectively transmitted to a human body; in addition, graphene can form a weak biological electric field on the surface of the human body to stimulate cell activity, and negative ions released by mineral substances are in contact with the skin of the human body to regulate the physiological function of the human body.

[Problem] To provide a method for producing a dendritic cell preparation for stimulating NKT cells from mononuclear cells collected from a beneficiary (mononuclear cells obtained by component blood collection or peripheral blood including mononuclear cells), said dendritic cell preparation exhibiting a faster effect than in the prior art. [Solution] A method for producing a dendritic cell preparation that returns to the body of a beneficiary who has been sampled, the method comprising: an adhesion step for adding the collected monocytes to a culture vessel using a liquid medium, leaving the vessel to stand, and causing some of the monocytes to adhere to the inner surface of the vessel; a non-adherent cell removal step for removing non-adherent cells including mononuclear cells other than the cells adhering to the inner surface of the culture vessel; a differentiation step in which a predetermined factor is added to the culture vessel and the monocytes adhering to the inner surface of the culture vessel are differentiated into immature dendritic cells; a pulsing step for pulsing alpha-galactosylceramide into the culture vessel in which the immature dendritic cells are present in a non-adherent state; and an NKT-stimulated dendritic cell induction step in which NKT-stimulated dendritic cells, which are dendritic cells that stimulate NKT cells, are induced from the immature dendritic cells.

In some embodiments, B cell proliferation and / or memory B cell formation / proliferation may be enhanced by contacting MAIT cells with Noogatointerleukin alpha due to chibercept (N-803) to produce stimulated MAIT cells, which in turn stimulate B cell proliferation and / or memory B cell formation / proliferation. Such stimulation may be performed in the presence of an antigen or antigen presenting cell. In particularly contemplated embodiments, MAIT cell stimulation is performed in vitro to produce an immunostimulatory composition and vaccine, or in vivo to enhance an immune response in airway tissue. Most typically, in vivo immune stimulation is carried out by inhalation or intranasal delivery of a composition comprising N-803 and optionally a vaccine component.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

Compositions and methods for stimulating NK cell expansion and cytotoxicity are described. Therapeutic compositions and methods using expanded and stimulated NK cells are described.

The present invention relates to a feeder cell for the activation and amplification of NK cells and use thereof. The feeder cell for NK cells expresses at least one of mbCD16, mbIL-12, and mbIL-18, along with HLA-E, thereby stimulating NK cells to increase the activation and amplification of the NK cells, and these NK cells can be used as a cell therapeutic agent and a pharmaceutical composition for the prevention or treatment of cancer or infectious diseases.

The application provides new compositions and methods for stimulating the production of natural killer (NK) cells in a subject. NK cells can be selectively expanded with a combination of stimulating ligands. Methods and compositions for the administration of stimulatory ligands modified to self-insert into tumor cells, thereby stimulating an increase in the number of NK cells in proximity to a tumor, are also described.

A proinflammatory cytokine production inhibitory apparatus for inhibiting production of a proinflammatory cytokine by magnetic stimulation of cells, the proinflammatory cytokine production inhibitory apparatus comprising:an electromagnetic wave production member to produce an electromagnetic wave at a proinflammatory cytokine production inhibitory frequency so as to expose the cells to an alternating magnetic field at the proinflammatory cytokine production inhibitory frequency;a frequency control member to control the proinflammatory cytokine production inhibitory frequency of the electromagnetic wave to 100 to 450 MHz so as to inhibit production of the proinflammatory cytokine by magnetic stimulation with the alternating magnetic field at the proinflammatory cytokine production inhibitory frequency; andan irradiation member to irradiate the cells with the alternating magnetic field at the proinflammatory cytokine production inhibitory frequency.

To provide a method for genetically engineering T cells for use in a cell therapy without cell expansion, as well as compositions of the generated T cells and uses thereof for treating a subject.SOLUTION: Disclosed is a method for producing a composition of engineered T cells, the method comprising the steps of: (a) exposing a composition comprising primary T cells to a stimulator under conditions to stimulate T cells thereby generating a stimulated population, where the stimulator activates one or more intracellular signaling domains of one or more components of TCR complex, and activates one or more intracellular signaling domains of one or more co-stimulatory molecules; (b) introducing to the T cells of the stimulated population a heterologous polynucleotide encoding a recombinant protein thereby generating a population of transformed cells; (c) incubating the population of transformed cells; and (d) following the incubation, harvesting T cells of the transformed cell population thereby producing a composition of the engineered cells.SELECTED DRAWING: Figure 13A

The invention relates to a three-specificity T cell adapter targeting HER2 (human epidermal growth factor receptor 2), HER3 (human epidermal growth factor receptor 3) and CD3 (human epidermal growth factor receptor 3) as well as a preparation method and application thereof. The three-specificity T cell adapter comprises an HER2 antigen binding structural domain, an HER3 antigen binding structural domain and a CD3 antigen binding structural domain which respectively target a human HER2 protein, a human HER3 protein and a human CD3 protein; the HER3 binding structural domain and the HER2 binding structural domain can target tumor cells expressing the two antigens, the CD3 binding structural domain can target T cells, the three-specificity T cell adapter can shorten the distance between the tumor cells and the T cells, and the T cells are stimulated to activate, so that the T cells have cytotoxic effect on the tumor cells; by combining the anti-HER3 binding structural domain and the anti-HER2 binding structural domain, on one hand, the action range and the action effect of the three-specificity T cell adapter can be increased, and on the other hand, the purposes of increasing specificity and reducing non-specific toxic and side effects can be achieved by targeting two different antigens of the same tumor cell at the same time.

The present invention provides an organ-on-a-chip with a multi-layer structure, which includes an upper flow channel layer, an upper porous membrane layer, a tissue layer, a lower porous membrane layer, and a lower flow channel layer that are stacked in sequence from top to bottom. The upper flow channel layer includes a first perfusion port, a second perfusion port, a first upper flow port, and a second upper flow port; the hydrogel containing cells is perfused into the first perfusion channel or the second perfusion channel through the first perfusion port or the second perfusion port. Under the blockage of the upper porous membrane layer and the lower porous membrane layer, the hydrogel containing cells fills the tissue chamber; then, cell culture medium is injected through the first upper flow port and the second upper flow port, and permeates into the tissue chamber through the first porous membrane and the second porous membrane respectively to culture cells. The present invention can separate the perfusion channel of the hydrogel containing cells from the perfusion channel of the cell culture medium, and the perfusion of the hydrogel containing cells will not block the channel of the cell culture medium, realizing uniform circulation of the cell culture medium in the organ-on-a-chip to stimulate cells.

The invention discloses a multifunctional friction micro-current textile and a preparation method thereof. According to a copper-containing conductive fiber layer, the copper content is larger than or equal to 2000 mg / kg, and a CuO nanocone array is arranged on the surface of the copper-containing conductive fiber layer; the graphene conductive fiber layer and the copper conductive fiber layer are woven in a staggered mode to form a friction interface, and the biological fiber layer accounts for 40-60% and provides skin-friendly performance and air permeability; the integrated micro-current generation system is used for generating 10-500 [mu] A pulse current through human body motion friction to stimulate cell repair and metabolism regulation (the integrated micro-current generation system is in accordance with a human body friction micro-current threshold value of IEC60601-1; the micro-current threshold value ranges from 0.5 mA to 10 mA, and accords with the IEC60479-1 safety contact current threshold value; the traditional Chinese medicine composition can stimulate cell repair, activate immune cells, improve microcirculation, inhibit lactic acid generation, inhibit inflammation and regulate metabolism.