4results about How to "The cultivation method is simple" patented technology

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The invention discloses bacillus velezensis BZ capable of degrading petroleum hydrocarbon, the name of the strain is BZ, the classification name is bacillus velezensis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31766. The invention further discloses a preparation method of the bacillus velezensis BZ capable of degrading petroleum hydrocarbon. The invention discloses an application of bacillus velezensis capable of degrading petroleum hydrocarbon in degradation of petroleum hydrocarbon. The BZ strain provided by the invention can grow by taking petroleum hydrocarbon as a sole carbon source, can tolerate the low temperature of 5 DEG C and salinity with the concentration of 10%, and can quickly degrade the petroleum hydrocarbon, so that the BZ strain can be widely applied to petroleum degradation. In addition, the strain is simple in culture method and high in tolerant petroleum hydrocarbon concentration, and can be directly used for treating petroleum-polluted water and soil.

The application discloses a kind of lentinus edodes factory directional single side cultivation method, belong to lentinus edodes cultivation technical field, the application adds hydrated silica in lentinus edodes liquid culture medium, effectively controls lentinus edodes ball size using its high friction value, make its flowability stronger, and sodium calcium phosphosilicate can effectively improve lentinus edodes mycelium amount.Red and blue double film bag is used simultaneously with the characteristics of light transmission and oxygen injection, causing the difference in maturity of the surface of the stick, so as to realize the mature face mushroom, non-mature face energy supply.Through the special mushroom cultivation layer frame setting stick independent liquid supplement component, it can not only increase the connection firmness of lentinus edodes stick and frame, but also realize liquid supplement on cultivation layer frame.The application overcomes the difference in evaporation speed of the mushroom surface caused by the stick layer block in the layer frame mode, significantly shortens the lentinus edodes cultivation period, realizes the stable synchronization of stick color change, directional mushroom, precise water replenishment, and has good economic benefits and application prospect.

This invention belongs to the field of environmental microbiology, specifically a type of Klebsiella variicola and its applications. Klebsiella variicola, strain IFPBD3, is deposited at the China General Microbiological Culture Collection Center (CGMCC) on May 25, 2023, with accession number CGMCC No. 27461. The rice straw degrading bacteria produced by the technical solution of this invention can effectively degrade rice straw, significantly increase straw fertility, and improve straw utilization rate.