545results about How to "The cultivation method is simple" patented technology

The invention discloses a method for reshaping pear trees, relating to a method for reshaping fruit trees and comprising the following steps: (1) determining reasonable plantation density, wherein appropriately, the line spacing is 4m and the row spacing is 5-6m; (2) formulating an overall objective for culturing tree shapes, controlling the trunk height of a pear tree to be 0.7-0.8m and the distance away from the ground to be 0.8m, allocating two boughs along a progressive direction, and inducing horizontally; and allocating lateral branches at the two sides of the boughs, performing line spacing inducement through a mode for forming a dip angle of 45 degrees with the horizontal plane, and controlling the spacing between lateral branches to be 0.4-0.5m; (3) realizing tree shape culture by the steps of bough culturing and inducing, and reasonable allocating and trimming of lateral branches; and (4) constructing a framework adaptive to labor-saving reshaping, wherein the pear trees are V-shaped and U-shaped. The invention has the advantages as follows: (1) the reshaping technology has simple requirements and rapid speed; (2) the field operation is easy and has high efficiency; (3) the expansion of operation to an appropriate scale per capita is possible; and (4) the early-stage yielding ability is good.

The invention relates to a method for culturing a mother spawn, a liquid spawn and a solid spawn of phlebopus portentosus. The method is characterized in that: raw materials such as potato, sucrose, glucose, agar, yeast extract, MgSO4, KH2PO4, rubber wood timber dust or other weed tree dust, grains and water are used to prepare culture media of the mother spawn, the liquid spawn and the solid spawn according to a certain proportion; and according to the culture method from the mother spawn, the liquid spawn to the solid spawn, the phlebopus portentosus mother spawn and liquid spawn are firstly cultured, and then the liquid spawn is inoculated on the solid spawn culture medium so as to culture the solid spawn. The culture mediums are particularly suitable for the culture of the phlebopus portentosus mother spawn, the liquid spawn and the solid spawn. The method can be used to carry out the rapid culture of the phlebopus portentosus spawns from the mother spawn, the liquid spawn to the solid spawn.

The invention discloses a high-mountain konjak cultivation technique, which comprises the steps of land selection, soil preparation, breeding konjak selection and treatment, due-time planting, field management, diseases and pests prevention and control, harvest and the like. According to the invention, by soaking seeds with a Chinese herbal medicine solution, weeding with a Chinese herbal medicine herbicide, and spraying a Chinese herbal medicine disease and pest preventing agent for prevention, diseases and pests of the konjak can be greatly reduced, the growth environment of the konjak is purified, the use of chemical pesticide is reduced, and the ecological environment is protected. The konjak produced by the cultivation technique is good in product appearance, safe and nuisance-free. The cultivation method is simple, has the advantages of low cost, high yield and the like, and is convenient to manage and suitable for large-scale cultivation.

The invention discloses an early-spring high-yield potato cultivation technology. The early-spring high-yield potato cultivation technology comprises the following steps: variety selection, potato seed processing, grading-based germination acceleration, planting land selection, land preparation and fertilization, sowing and seedling raising, field management, control of pest and disease, harvesting and the like. According to the invention, by means of immersing seeds with a Chinese medicinal herbal solution and carrying out preventive spraying by using a Chinese medicinal herbal agent for preventing diseases and pests, disease and pest damages to potatoes can be reduced greatly, the growth environment of the potatoes is purified, the utilization of chemical pesticides is reduced, and an ecological environment is protected. The potatoes planted according to the invention are good in grade and are safe and pollution-free. The cultivation technology disclosed by the invention is simple, has the advantages of low cost, high yield, convenience for management and the like, and is suitable for large-scale cultivation.

The invention discloses a method for cultivating potatoes. The method comprises the following cultivating steps of selection of cultivating environment, arrangement of crops for rotation, variety choosing, pre-sowing preparation, field management, top dressing, control of diseases and insects, harvesting and the like. The method has the advantages of simplicity, low cost, high yield, suitability for large-scale cultivation and the like, and is convenient to manage.

The invention discloses a seed reproduction and high-yield cultivation technical specification of onion, which aims at providing the seed reproduction and high-yield cultivation technical specification of the onion against the problem that the prior seed reproduction of the onion is lack of the quipped cultivation technical measures, so that the seed production is low and unstable. The seed reproduction according to the specification has the advantages of simple and scientific cultivation method, high seed production, labor-saving property, low cost, stable seed nature and high purity.

The invention relates to a method for artificially culturing fuscous dictyostelium boletes in mushroom houses, greenhouses, field and nursery. The method is characterized in that firstly bolete rods are cultured, then (1) the bolete rods are earthed up to culture mushroom in mushroom houses and greenhouses, fuscous dictyostelium boletes substance can be grown and developed to mature after 10-15 days of earthing; (2) the bolete rods are debagged and earthed up to culture mushroom in mushroom houses and greenhouses, and fuscous dictyostelium boletes substance can be grown and developed to mature after 20-25 days of earthing; (3) solid bolete strains are inoculated at the roots of tropical cash crops and flower plants planted in fields to culture mushroom, and fuscous dictyostelium boletes substance can be grown and developed to mature after 30-60 days of inoculation; artificial culture of fuscous dictyostelium boletes and the industrialization production of the fuscous dictyostelium boletes can be fast realized. The culture method is simple and convenient, the culture period is short and the fruiting is regular and the yield is high.

The invention relates to the field of bioengineering technology, and in particular to a method for in-vitro induction of cholangiocyte-like transformation of primary hepatocytes and for long-term culture, amplification and differentiation and an application of the method. The invention provides a hepatocyte cholangiocyte-like transformation medium determined by chemical ingredients and / or a system which is composed of a hepatocyte mature medium and is applicable to long-term stable culture, amplification and differentiation of the primary hepatocytes; the invention also provides the method for in-vitro induction of cholangiocyte-like transformation of the primary hepatocytes and for long-term culture, amplification and differentiation; and with the application of the method, the cholangiocyte-like hepatocyte conversion of the primary hepatocytes can be induced in vitro, so that the obtained hepatocytes have characteristics of biliary epithelial cells and hepatic precursor cells, and the hepatocytes are applicable to long-term stable culture and amplification. The breedable cholangiocyte-like hepatocytes and hepatocytes, which are mature in differentiation, prepared by the invention are applicable to such aspects as toxicologic and pharmacological evaluation of compounds and drugs, researches and diagnosis & treatment of hepatitis viruses, treatment by hepatocyte transplantation, preparation of bioartificial liver and the like.

The invention discloses a method for cultivating toadstools. The method comprises the following steps that 1, compost is prepared; 2, sterilization is carried out; 3, inoculation is carried out; 4, mycelium culture is carried out, wherein culture temperature ranges from 20 DEG C to 22 DEG C, air humidity ranges from 45 percent to 55 percent, and after ten days, the temperature ranges from 15 DEG C to 17 DEG C, and the air humidity ranges from 45 percent to 50 percent; 5, land treatment is carried out; 6, a sunshade is built; 7, secondary plowing is carried out; 8, field breeding is carried out; 9, final-period management is carried out, wherein in the earlier stage, air humidity in the sunshade ranges from 30 percent to 35 percent, soil humidity ranges from 55 percent to 65 percent, and temperature ranges from 5 DEG C to 16 DEG C, after 85 days, the air humidity ranges from 83 percent to 88 percent, the soil humidity ranges from 85 percent to 90 percent, the temperature ranges from 22 DEG C to 25 DEG C, and in the fruiting process, the air humidity ranges from 60 percent to 70 percent, the soil humidity ranges from 75 percent to 80 percent, and the temperature does not exceed 28 DEG C; 10, harvesting is carried out. The method is short in period, the contamination rate of the cultivated toadstools is low, the yield is high, and quality is good.

The invention relates to a Phlebopus portentosus cultivating method. The method comprises the steps of culture medium preparing, bacterium inoculating, earthening, mycelium culture in an earthening layer and fruiting cultivating. The invention further relates to a fungus culture medium and the purpose of the fungus culture medium in Phlebopus portentosus cultivating.

The invention relates to a sugarcane node bud seedling raising and cultivating method. The sugarcane node seedling raising and cultivating method sequentially comprises the steps of: (1) selecting sugarcane node buds and carrying out pretreatment; (2) putting the pretreated sugarcane node buds into a sprouting machine for sprouting, or burying the pretreated sugarcane node buds for sprouting, wherein in the process of sprouting, the temperature of the surroundings is maintained to be 30 DEG C to 40 DEG C, and the humidity is maintained to be 40% to 60%; (3) after the white part of partial bud eyes of the sugarcane node buds bud is exposed, implanting the sugarcane node buds into a seedling cup filled with a seedling matrix, and then putting the seedling cup into a greenhouse for continuous sprouting and cultivating, wherein the temperature in the greenhouse is maintained to be 28 DEG C to 35 DEG C, and the humidity is maintained to be 40% to 50%; and (4) when sprouts of the sugarcane node buds in the seedling cup grow out of the seedling cup, implanting the sugarcane node buds together with the seedling cup into the ground for planting. The sugarcane node bud seedling raising and cultivating method has the advantages of simplicity, practicality, reduction of planting cost and improvement of sugarcane output and is quite suitable for wide rural sugarcane growers to plant sugarcanes.

The invention discloses paracoccus and the application for degrading azotic heterocyclic compound. The paracoccus is paracoccus sp. BW001 CGMCC No. 2225. The paracoccus sp. BW001 CGMCC No. 2225 of the invention, on one hand, can grow into pyridine as the only carbon source and nitrogen source, which has a strong degradation activity (the degradation rate is 100%) for pyridine, is extremely suitable for biologic control of organic wastewater (such as coking wastewater) which contains pollutant of pyridine category, and can be used as biological hardening agent in the biological control technology of organic persistent wastewater, and corresponding environmental biological agent is exploited, thereby having relatively high value of study, application and marketing.

The invention relates to a culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte, and aims to solve the problem that the prior art is low in differentiation rate. The culture method comprises the following steps: 1) performing separation of primary cells of adipose tissue-derived stromal cells; 2) performing amplification and passage of the adipose tissue-derived stromal cells; and 3) performing differentiation culture on P5-generation adipose-derived stem cells to chondrocyte, namely, adding the adipose tissue-derived stromal cells into a condition culture medium, namely, an adipose tissue-derived stromal cell chondrocyte differentiation culture medium, after P5 generation of passage, performing chondrocyte differentiation culture, replacing the medium of the cells every 3 days, observing the morphological change of the cells, and after 16 days of induction, performing Alcian blue dyeing, and identifying the chondrocyte differentiation situation of the adipose tissue-derived stromal cells. The result of Alcian blue dyeing identification shows that the chondrocyte can be formed, and the Alcian blue dyeing is positive. The culture method has the characteristics of being simple and feasible, short in induction time as the induction culture medium is a serum-free culture system, good in test repeatability and high in osteoblast differentiation rate.

The invention relates to a method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo, comprising the following steps of: (1) culturing mycorrhizal fungi by utilizing a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP2 deuteromycotina Fusariumsp, mycorrhizal fungi LP3, or mixed strain deuteromycotina Verticilliumsp of the mycorrhizal fungi LP2 deuteromycotina Fusariumsp and the mycorrhizal fungi LP3; (2) culturing tissue culture seedlings of the dendrobium officinale kimura et migo; (3) preparing a nutrient solution; (4) carrying out water culture and training the tissue culture seedlings; and (5) carrying out planting and mycorrhizalculturing of the dendrobium officinale kimura et migo. The method for culturing, planting and mycorrhizal production of the dendrobium officinale kimura et migo, provided by the invention, can promote growth of dendrobium officinale kimura et migo seedlings obviously; and compared with a contrast plant, the dendrobium officinale kimura et migo cultured by inoculating pathogen has increased rooting numbers, high survival rate, longer plant length, better growth vigor, obviously expanded internodes, obviously big stems, improved fresh weight and dry weight and obviously enhanced stress resistance of a pathogen-inoculated plant.

The invention discloses a flexible ecological floating bed relating to a plant growth carrier. The invention provides a flexible ecological floating bed which has better landscape effect, has the advantages of small volume, light weight, no fixed frame, large buoyancy and the like, can be applied to lakes and riverways on large area, can be applied on ecological comprehensive treatment of water areas, such as salt-water inner bays, lagoons and the like. The flexible ecological floating bed is provided with a bed body and cultivated plant, wherein the bed body is provided with an upper-layer film coating woven fabric and a lower-layer film coating woven fabric; double layers of air bubble films are arranged between the upper-layer film coating woven fabric and the lower-layer film coating woven fabric; the periphery of the bed body is provided with a floating body bag along the edge; two side bags of the floating body bag are respectively provided with a floating bottle; bags on the head and the tail of the floating body bag are inserted with bamboo poles for shaping; the bed body is provided with a cultivation hole (or a field planting hole) for cultivating plant; plants are cultivated in the cultivation hole; and the periphery of the bed body is provided with fixing rings.

The invention relates to bradyrhizobium japonicum capable of effectively fixing nitrogen and a culture method and application thereof. The bradyrhizobium japonicum CGMCC No.4346 is cultured by the steps of strain activation, seed culture, fermentation culture and the like. The invention has the following beneficial effects: sorbitol and diethylene glycol are utilized to replace the carbon source, mannitol in the traditional YEM (yeast extract mannitol) culture medium, and when the improved fermentation culture medium is adopted for culturing, the viable count of the fermentation liquor is higher than that of the YEM culture medium; compared with the rhizobium inoculants which are not inoculated on the soy beans, the bradyrhizobium japonicum agent ensures the theoretical yield of the soy beans to be increased by 16.7-17.2%, the crude fat content to be increased by 0.65-1.21% and the protein content to be increased by 1.29-1.60% after being inoculated on the soy beans, thus being an excellent strain for production of rhizobium japonicum and inoculation of the soy beans; and the culture method is simple and practical, and the raw materials have wide sources and low price and are suitable for large-scale industrialized production.

The invention belongs to the technical field of the biological pesticide and particularly relates to a biocontrol bacterium for controlling plant diseases and a preparation method thereof. The invention has the following main contents: (1) the separation and identification of Bacillussp.CC09 strain; (2) the preparation of Bacillussp.CC09 strain biocontrol microbial inoculum and the applications of the microbial inoculum in the control of plant diseases; and (3) the stability of the Bacillussp.CC09 biocontrol microbial inoculum. The Bacillussp.CC09 biocontrol microbial inoculum is characterized in that the anti-fungal spectrum is wide, the effect is long-lasting, harmful bacteria are difficult to generate drug resistance, the microbial inoculum is safe for human, livestock and crops and is environmentally friendly, etc. The biocontrol bacterium has obvious inhibitory activity for the growth of Fusariumgraminearum, Alternariaalternata, Rhizoctoniasolani and Phytophthoracapsici and especially obvious control effect on wheat powdery mildew and scab.

The invention discloses a method for cultivating peach trees in a greenhouse. The method includes the following steps that first, the plastic greenhouse is established; second, variety selection is performed, and cultivating density is determined; third, young trees are managed; fourth, the time when the greenhouse is covered with a thin film is determined; fifth, management in the greenhouse, such as illumination control, flower and fruit management, tree body management and fertilizer and water management, is performed; sixth, management performed after the thin film is removed from the greenhouse is performed. Due to the method for cultivating the peach trees in the greenhouse, the growing speed is high, yield is high, quality is good, the sugar content of fruits is high, and the fruits taste good; the cultivating method is simple, convenient to operate and good in economic benefit and has very high promotional value.

The invention discloses an early spring tomato seedling and greenhouse cultivation method and relates to the technical field of tomato cultivation. The method mainly comprises the following steps of (1) variety selection, (2) seedbed conditioning, (3) seed soaking and disinfection, (4) sowing and seedling raising, (5) provisional planting, (6) soil preparation and fertilization, (7) field planting, (8) field management, (9) pruning flower-reserving and fruit grading and thinning, and (10) pest control. The cultivation method provided by the invention has the advantages that the cultivation method is simple and easy to implement, is applicable to early spring tomato seedling and greenhouse cultivation, the method realizes low investment and high yield, the occurrence rate of plant diseases and insect pests is low, and the product quality and the market competitiveness are improved.

The invention provides a hypsizigus marmoreus culture medium which comprises the following components in percentage by mass: 40-50% of corn cob, 8-15% of rice bran, 3-8% of schisandra fruit dreg, 10-20% of radix salviae miltiorrhizae dreg, 5-10% of soybean hull, 12-20% of wheat bran, 0.8-2% of shell powder and 0.2-1% of lime. The invention belongs to the technical field of edible fungus culture. The hypsizigus marmoreus culture medium provided by the invention does not need wood dust or cottonseed hull, fully utilizes the schisandra fruit dreg and radix salviae miltiorrhizae dreg, and thus, has the advantages of wide raw material sources, high air permeability, favorable culture effect and the like on the premise of lowering the cost.

The invention relates to a semi-artificial culture method of phlebopus portentosus. The method includes the following steps: firstly, culturing delonix regia, artocarpus heterophyllus lam seedling andgrapefruit grafted seedling; then transplanting the delonix regia, the artocarpus heterophyllus lam seedlings and the grapefruit grafted seedlings to a field; and when age of the tree reaches 1 year,inoculating the solid strain of phlebopus portentosus on the root systems of the delonix regia, an artocarpus heterophyllus lam treelet and a young or mature grapefruit. After one year of the inoculation, the fruiting bodies of phlebopus portentosus grow under the delonix regia, the artocarpus heterophyllus lam tree and the grapefruit tree and develop mature, and more than 10-80kg of phlebopus portentosus can be produced per 100m<2>. The use of the method can rapidly realize the semi-artificial culture of phlebopus portentosus, as well as industrialization. The semi-artificial culture methodof phlebopus portentosus is simple, saves the process of artificially synthesizing the root source of bacteria, is short in fruiting time, simultaneously harvests economical crop fruits and the fruiting body of boletus, realizes the common harvest of fruits and bacteria, greatly increases output, saves capitals and other corresponding growing cost and improves land use capability.

The invention discloses a pollution-free high-yield cultivation method for Chinese onions. The method comprises the first step of sowing and seedling raising, the second step of transplanting and management after transplanting, the third step of prevention and control of diseases and pests and the fourth step of harvest and storage of Chinese onions. The pollution-free high-yield cultivation method for Chinese onions is simple, operability is high, farmers can easily grasp the cultivation method, the yield is high, the benefit is good, and the method has extremely high application and popularization value.

The invention relates to a cultivation method of edible chrysanthemum flowers, and belongs to the technical field of chrysanthemum flower cultivation. The method particularly comprises the steps that weakly acidic or neutral fertile sandy loam soil is selected, base fertilizer is applied sufficiently, and deep ploughing, airing, flattening and fine harrowing are conducted; at the beginning of May, branches which are better in growth vigor are selected, cutting seedlings are pruned, and seedling raising is conducted in a shading shed; when the cutting seedlings take roots and each sends forth two completely-spread new leaves, young seedling transplanting is conducted; work such as water controlling, fertilizer application and tip pruning needs to be paid attention to after transplanting is conducted, cultivating and weeding need to be well conducted, and the disease and insect preventing work needs to be well conducted; in the period from the end of October to the end of November, harvesting is conducted on sunny days, and the processing work and the storage work need to be well conducted on the harvested chrysanthemum flowers. The cultivation method is simple, planting of the edible chrysanthemum flowers is conducted through the cultivation method, the chrysanthemum flowers which are high in yield and quality can be obtained, the standard of the edible chrysanthemum flowers is met, and the produced chrysanthemum flowers are rich in selenium, can provide and supplement selenium for human bodies and are high in commodity value and beneficial for popularization.

The invention discloses a cultivation method for high-yield potatoes. The method includes the steps of field selection, basal dressing, seed selection, sterilization and bud forcing, sowing, field management and prevention and control of insect pests and weeds. The cultivation method has the advantages of being simple, low in cost, high in yield, convenient to manage and the like, and is suitable for large-scale cultivation.

The invention belongs to the technical field of vegetable cultivation and particularly relates to a cherry tomato cultivation method. The cherry tomato cultivation method comprises the following steps of selecting vegetable plots, erecting protective greenhouses, raising seedlings in advance, preparing lands and performing timely transplantation, field management and fruit harvesting. The vegetable plot selecting step is that selected lands are plain, convenient to irrigate and drain, good in ventilation and light transmission and loose and fertile in soil or sandy soil and are plots where the same kinds of previous-crop vegetables are not planted. The protective greenhouse erecting step is that the protective greenhouses with the height of 3-4 meters are erected, automatic water spraying devices and temperature adjusting devices are arranged in the protective greenhouses, and heat-preservation films are arranged outside greenhouses. The cherry tomato cultivation method has the advantages of being simple, free of pesticide residues, environmental-friendly, free of pollution, high in yield, convenient to manage and the like and is suitable for large-scale cultivation.

The invention relates to a cultivation method for planting summer cress in a vegetable greenhouse. The cultivation method comprises the following steps of: (1) selecting high-temperature-resistant variety; (2) forcing germination of seed stems; (3) covering with a 50-70% sunshade net; (4) plowing and finishing field surface to be flat, smooth, mashed and mature; (5) spreading and arranging the seed stems; (6) covering with rice straw; (7) tapping furrow surface with a square hoe or wood board so that the seed stems and the rice straw are coated by a layer of slurry; (8) scientifically managing and controlling fertilizer and water; (9) spraying gibberellin and additionally fertilizing at roots 4-7 days before harvesting; and (10) picking in proper stage. According to the cultivation method provided by the invention, by covering with the sunshade net, cool microclimate is built; by covering with rice straw and slurry, the survival rate of the seed stems is improved; by supplying enough fertilizer, the vegetative growth of cress is promoted; and by spraying gibberellin, the summer cultivation difficulty of cress is better solved. In addition, by adopting the cultivation method provided by the invention, water and drought crop rotation in the vegetable greenhouse is realized, and a simple and efficient new way is found for preventing and controlling continuous cropping drawback.

The invention discloses a strain of obligate halophile Y-B, which is obtained from well drilling waste mud by enrichment, screening and culture, and aims at realizing high-efficient degradation of petroleum in high-salt oily waste liquor in the petroleum industry. The strain Y-B is milky white, is of a gram-positive bacterium, is shaped like a cell rod and can produce spores, the strain Y-B is identified as Bacillus subtilis subsp. spizizenii and has very high activity, a culture method is simple, and the strain Y-B has the advantages of easiness in obtainment, low cost and rapid growth, is suitable for weakly-alkaline and high-salt environments, has a better effect of removing the petroleum in the high-salt oily waste liquor and further has broad application prospects in the treatment ofthe high-salt oily waste liquor in the petroleum industry and oil overflow sudden accidents.

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention discloses a China rose batch cultivating method. The cultivating method is characterized by comprising the following steps of (1) pretreatment of China rose branches; (2) cuttage; (3) fertilizer applying before transplanting; (4) transplanting for the first time; (5) stem fixing of a single seedling; (6) transplanting for the second time; (7) stem fixing; (8) maturing. The cultivating method has the advantages that the problems of multiple branches and short and small corolla of the original China rose are solved; after nutrient cultivating for long years, the flower season of China rose which is cultured by the method is longer, the diameter of the main stem and the width of the corolla are obviously increased, the main stem is upright, tall and straight, the flower petal is larger, multiple colors and shapes are realized, and the beautiful effect of the garden plants is enriched; the diversity of trees in the garden greening is increased; the cultivating method is simple, the learning is easy, the operation difficulty is low, and the cultivating method is favorable for being popularized and applied.

The invention relates to primary culture and identification methods for smooth muscle cells of esophagogastric junction via enzyme digestion method. The primary culture method includes steps of: 1) acquiring a muscle strip of smooth muscle tissue of the esophagogastric junction; 2) digesting the muscle strip of the smooth muscle tissue with a digestion liquid to prepare a smooth muscle tissue cellsuspending liquid; 3) placing the smooth muscle tissue cell suspending liquid in a culture container, in which levo-polylysine is pre-paved, and culturing the cells until adherent cells account for more than 90% of the bottom of the container or cells grow crowdedly locally. The primary culture method in the invention is simple, completed and effective. The identification method can identify thespecial phenotypes of the smooth muscle cells.