17results about How to "The cultivation method is simple" patented technology

The invention discloses a metarhizium aureoviride strain YEC and application of the metarhizium aureoviride strain YEC in prevention and treatment of teak camel moths. The Metarhizium anisoviride YEC provided by the invention is separated from soil in Guangdong province, can infect mangrove forest pests, namely teak camel moths, and can have relatively high fatality rate and stiff insect rate on the teak camel moths within a short time. The metarhizium aureoviride strain YEC is a biological control strain with potential development and application value in control of mangrove forest pests.

The invention discloses a culture method for improving the reproductive capacity of indigenous nematodes and application of the culture method, and belongs to the technical field of indigenous nematode culture. According to the present invention, the Rosselomyces aquimaris L-3 strain is the indigenous nematode intestinal tract strain, is the new strain, can enrich the nutrition source of the indigenous nematode, can significantly improve the egg laying amount of the nematode fed with the strain in the early stage of the breeding period, and can improve the egg laying amount by 36.3% compared with the currently common E. coli OP50 bacteria, and can not produce any harm to the indigenous nematode; the provided culture method is convenient and efficient, the breeding speed of indigenous nematodes can be remarkably increased under the laboratory environment condition by adopting the culture method provided by the invention, and compared with the currently common E. coli OP50 bacteria, the extremely remarkable difference can be achieved in the second breeding day, the necessary nematode number can be provided for short-period experiments, and the related research process can be accelerated.

The invention belongs to the technical field of bioengineering, and particularly discloses an in-vitro amplification method of canine NK cells capable of efficiently killing tumor cells and application of the canine NK cells. 3 mL of dog peripheral blood is taken, and dog peripheral blood mononuclear cells PBMC are obtained through separation; activating, amplifying and culturing canine NK cells capable of efficiently killing tumor cells; and collecting the amplified canine NK cells capable of efficiently killing the tumor cells, and storing the canine NK cells in a cryopreservation solution according to the cell density of 1 * 10 < 8 > cells / mL. According to the method, the canine NK cell with high amplification multiple can be obtained by only needing 3mL of canine peripheral blood, and the amplification multiple is high; the number of the dog NK cells of the healthy volunteer dog sample is 3.02 * 108, the amplification multiple is 1008, the total number of the dog NK cells of the tumor volunteer affected dog sample reaches 6.84 * 107, and the amplification multiple is 340; and the canine NK cell obtained by the invention has the effect of efficiently killing tumor cells.

The invention provides a method for inducing DC precursor cells in PBMC (peripheral blood mononuclear cells) to differentiate into pDC cells in vitro, which comprises the following steps: the PBMC is placed in a culture medium for culture, and the culture medium comprises the following components: GM-CSF (granulocyte-macrophage colony-stimulating factor), Poly (I: C), Flt3l, TNF-alpha, ODN2006, Compound 4, autologous plasma and an AIM-V basal culture medium; replacing or supplementing the culture medium based on the culture progress until the preset culture time is reached; and collecting the cultured cells. According to the method, the PBMC serves as a culture object, the DC precursor cells in the PBMC are finally differentiated into the pDC cells through culture, the culture method is simple, a large number of pDC cells can be conveniently and efficiently obtained in vitro, and guarantee is provided for basic research and clinical application of the pDC cells. Meanwhile, the pDC cells obtained after the culture medium is used for culturing for 7 days can secrete IFN-alpha with a relatively high level under the condition of no target cell stimulation, so that the application of the culture medium in antiviral and anti-tumor treatment is facilitated.

The present invention relates to the field of biological control technology, in particular to Roberts green muscardine and its application in biological control and growth promotion. The present invention provides Roberts green muscardine and a method for using the same for biological control. The strain is a green muscardine with high toxicity and insecticidal function. The strain has a simple cultivation method, rapid growth, and a large spore production. The mortality rate of infected locusts can reach 100.00%, and it is non-toxic and non-polluting, will not damage the ecology, is not easy to produce drug resistance, and is harmless to humans. It can be widely used in the prevention and control of locusts.

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The invention provides a desert chlorella culture medium as well as a use method and application thereof, and belongs to the technical field of desert chlorella culture. Each liter of the culture medium for the chlorella desert comprises the following components: 90 to 100 milligrams of NaNO3, 20 to 30 milligrams of KH2PO4, 20 to 30 milligrams of K2HPO4, 2 to 3 milligrams of MgSO4. 7H2O, 7 to 8 milligrams of CaCl2. 2H2O, 2 to 3 milligrams of NaCl, 4 to 6 milligrams of Na2EDTA. 2H2O and 0 to 5 milligrams of KOH, and the optimal pH value of the culture medium is 6.5 to 7.5. The growth speed of the desert chlorella in the desert chlorella culture medium is obviously higher than that of other culture media, and the culture medium and the culture method are simple, convenient and easy to operate, green and environmentally friendly, can be beneficial to later-stage large-scale culture of the desert chlorella, and are of great significance to preparation of chlorella related products.

The invention relates to the technical field of polyploid cultivation, in particular to a cultivation method and application of octaploid actinidia arguta. The invention relates to an octaploid actinidia arguta culture method, which comprises: (1) soaking tetraploid actinidia arguta seeds in a colchicine solution for 20-28 h to obtain treated seeds; (2) washing the treated seeds, draining, and culturing until germination to obtain germinated seeds; and (3) sowing and cultivating the germinated seeds to obtain the octaploid actinidia arguta. According to the culture method disclosed by the invention, octaploid actinidia arguta can be successfully induced by using tetraploid actinidia arguta 'Longxian No.2', and the culture method is simple and suitable for wide popularization.

The invention discloses bacillus velezensis BZ capable of degrading petroleum hydrocarbon, the name of the strain is BZ, the classification name is bacillus velezensis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31766. The invention further discloses a preparation method of the bacillus velezensis BZ capable of degrading petroleum hydrocarbon. The invention discloses an application of bacillus velezensis capable of degrading petroleum hydrocarbon in degradation of petroleum hydrocarbon. The BZ strain provided by the invention can grow by taking petroleum hydrocarbon as a sole carbon source, can tolerate the low temperature of 5 DEG C and salinity with the concentration of 10%, and can quickly degrade the petroleum hydrocarbon, so that the BZ strain can be widely applied to petroleum degradation. In addition, the strain is simple in culture method and high in tolerant petroleum hydrocarbon concentration, and can be directly used for treating petroleum-polluted water and soil.

The present application belongs to the field of microbial technology, and specifically designs a Planococcus dechangensis strain that can efficiently degrade sirbucil under high-salt conditions, and names it XA-9. Planococcus dechangensis XA-9 can tolerate a maximum salinity of 50-60 g / L NaCl; it completely degrades sirbucil not higher than 30.0 mg / L within 28-36 hours, and the 36-hour degradation rate of sirbucil at 40.0-60.0 mg / L is as high as 81.9%-99.6%, respectively, indicating that it can be used for the remediation of high-salt wastewater containing sirbucil.

The application discloses a kind of lentinus edodes factory directional single side cultivation method, belong to lentinus edodes cultivation technical field, the application adds hydrated silica in lentinus edodes liquid culture medium, effectively controls lentinus edodes ball size using its high friction value, make its flowability stronger, and sodium calcium phosphosilicate can effectively improve lentinus edodes mycelium amount.Red and blue double film bag is used simultaneously with the characteristics of light transmission and oxygen injection, causing the difference in maturity of the surface of the stick, so as to realize the mature face mushroom, non-mature face energy supply.Through the special mushroom cultivation layer frame setting stick independent liquid supplement component, it can not only increase the connection firmness of lentinus edodes stick and frame, but also realize liquid supplement on cultivation layer frame.The application overcomes the difference in evaporation speed of the mushroom surface caused by the stick layer block in the layer frame mode, significantly shortens the lentinus edodes cultivation period, realizes the stable synchronization of stick color change, directional mushroom, precise water replenishment, and has good economic benefits and application prospect.

This invention belongs to the field of environmental microbiology, specifically a type of Klebsiella variicola and its applications. Klebsiella variicola, strain IFPBD3, is deposited at the China General Microbiological Culture Collection Center (CGMCC) on May 25, 2023, with accession number CGMCC No. 27461. The rice straw degrading bacteria produced by the technical solution of this invention can effectively degrade rice straw, significantly increase straw fertility, and improve straw utilization rate.

The present invention provides a recombinant genetically engineered bacterium of type A botulinum toxin and its preparation and application, belonging to the field of genetic engineering, the recombinant genetically engineered bacterium includes a pET‑28a vector, the pET‑28a vector includes a gene fragment 1 expressing a GST tag, a gene fragment 2 expressing a thrombin recognition site, and a gene fragment 3 expressing BoNT / A; the amino acid sequence of the GST is SEQ ID NO.1; the amino acid sequence of the thrombin recognition site is SEQ ID NO.2; the amino acid sequence of BoNT / A is SEQ ID NO.3. The recombinant genetically engineered bacterium provided by the present invention can produce type A botulinum toxin quickly and in large quantities. The present invention also optimizes the culture method of the recombinant genetically engineered bacterium, and increases the yield of type A botulinum toxin by adding an inducer. The present invention also optimizes the purification process of type A botulinum toxin, and prepares high-purity type A botulinum toxin.

The invention relates to a microbial agent capable of slowing down phytotoxicity of tobacco herbicides and a preparation method and application of the microbial agent, the microbial agent is obtained by compounding bacterial liquid on a carrier, and the bacterial liquid comprises Arthrobacter paraffineus, Bacillus cereus and Bacillus licheniformis. The microbial agent is a microbial agent capable of slowing down phytotoxicity of tobacco herbicides and a preparation method and application of the microbial agent capable of slowing down phytotoxicity of tobacco herbicides and a preparation method of the microbial agent capable of slowing down phytotoxicity of tobacco herbicides and a preparation method of the microbial agent capable of slowing down phytotoxicity of tobacco herbicides and a preparation method of the microbial agent capable of slowing down phytotoxicity of tobacco herbicides. The microbial inoculum forms dominant bacterial communities around tobacco seedling root systems, rapidly propagates, increases the generation of growth regulatory elements in plants, promotes root system development, enhances root system activity, reduces the influence of herbicide phytotoxicity, improves the growth vigor of tobacco plants, is beneficial to early growth and rapid germination of the tobacco plants, and has a wide application prospect.

The present invention belongs to the technical field of fungal cultivation, and specifically relates to a Blackwell Cordyceps strain and a cultivation method of its artificial fruiting body. The Blackwell Cordyceps (Cordyceps blackwelliae) strain ZYJ0835 was deposited in the China Center for Type Culture Collection on May 18, 2022, with a deposit number of: CCTCC NO: M 2022663; the deposit address is: Wuhan University, Wuhan, China. The mycelium and fermentation liquid processed products obtained by shaking culture of the strain ZYJ0835 have antioxidant, fibrinolytic, antibacterial and other activities, and fruiting bodies can be cultivated on substrates such as rice, wheat, and tussah pupae, and the obtained artificial fruiting bodies also have antioxidant, fibrinolytic and other activities. The cultivation method of the Cordyceps fruiting body of the present invention is simple, the cultivation conditions are easy to control, and industrial cultivation can be carried out, providing a new type of artificially cultivated Cordyceps resource.

The invention discloses a lignin degradation complexing agent. The lignin degradation complexing agent is prepared from haematococcus pluvialis and bacteria TD18, wherein the cell concentration of the haematococcus pluvialis is 5 * 10 < 5 > cell * mL <-1 >, and the cell concentration of the bacteria TD18 is 5 * 10 < 6 > cell * mL <-1 >; the method comprises the following steps: preparing a haematococcus pluvialis seed solution and a bacteria TD18 seed solution, and then mixing the haematococcus pluvialis seed solution and the bacteria TD18 seed solution according to a cell quantity ratio of 10: 1-10: 6, and inoculating the mixture into sewage containing lignin. The complexing agent can tolerate lignin and can synergistically and efficiently remove lignin and nitrogen in sewage, the sewage treatment effect is good, by means of the lignin degradation complexing agent, the removal rate of the lignin in the sewage is 8.42%-100%, and the removal rate of the lignin is 5.61 mg.( L.d) <-1 >-41.67 mg.( L.d) <-1 >; meanwhile, the culture method is simple, low in cost and suitable for preparing products for sewage treatment.

The present invention relates to a method for improving the viability of NK cells under high-density culture conditions. Specifically, the present invention can significantly improve the viability of NK cells by adding dasatinib to the NK cell culture system, without affecting cell expansion, and the cell's killing activity is better after cryopreservation and resuscitation. The method of the present invention solves the common problems of decreased NK cell viability during high-density expansion and decreased tumor cell killing activity after cryopreservation and resuscitation without changing the process route. It has the characteristics of simple operation, low cost, and no dependence on process methods, and has broad application prospects in the field of producing NK products.