545results about How to "The cultivation method is simple" patented technology

The invention discloses a method for reshaping pear trees, relating to a method for reshaping fruit trees and comprising the following steps: (1) determining reasonable plantation density, wherein appropriately, the line spacing is 4m and the row spacing is 5-6m; (2) formulating an overall objective for culturing tree shapes, controlling the trunk height of a pear tree to be 0.7-0.8m and the distance away from the ground to be 0.8m, allocating two boughs along a progressive direction, and inducing horizontally; and allocating lateral branches at the two sides of the boughs, performing line spacing inducement through a mode for forming a dip angle of 45 degrees with the horizontal plane, and controlling the spacing between lateral branches to be 0.4-0.5m; (3) realizing tree shape culture by the steps of bough culturing and inducing, and reasonable allocating and trimming of lateral branches; and (4) constructing a framework adaptive to labor-saving reshaping, wherein the pear trees are V-shaped and U-shaped. The invention has the advantages as follows: (1) the reshaping technology has simple requirements and rapid speed; (2) the field operation is easy and has high efficiency; (3) the expansion of operation to an appropriate scale per capita is possible; and (4) the early-stage yielding ability is good.

The invention discloses a high-mountain konjak cultivation technique, which comprises the steps of land selection, soil preparation, breeding konjak selection and treatment, due-time planting, field management, diseases and pests prevention and control, harvest and the like. According to the invention, by soaking seeds with a Chinese herbal medicine solution, weeding with a Chinese herbal medicine herbicide, and spraying a Chinese herbal medicine disease and pest preventing agent for prevention, diseases and pests of the konjak can be greatly reduced, the growth environment of the konjak is purified, the use of chemical pesticide is reduced, and the ecological environment is protected. The konjak produced by the cultivation technique is good in product appearance, safe and nuisance-free. The cultivation method is simple, has the advantages of low cost, high yield and the like, and is convenient to manage and suitable for large-scale cultivation.

The invention discloses a seed reproduction and high-yield cultivation technical specification of onion, which aims at providing the seed reproduction and high-yield cultivation technical specification of the onion against the problem that the prior seed reproduction of the onion is lack of the quipped cultivation technical measures, so that the seed production is low and unstable. The seed reproduction according to the specification has the advantages of simple and scientific cultivation method, high seed production, labor-saving property, low cost, stable seed nature and high purity.

The invention relates to a method for artificially culturing fuscous dictyostelium boletes in mushroom houses, greenhouses, field and nursery. The method is characterized in that firstly bolete rods are cultured, then (1) the bolete rods are earthed up to culture mushroom in mushroom houses and greenhouses, fuscous dictyostelium boletes substance can be grown and developed to mature after 10-15 days of earthing; (2) the bolete rods are debagged and earthed up to culture mushroom in mushroom houses and greenhouses, and fuscous dictyostelium boletes substance can be grown and developed to mature after 20-25 days of earthing; (3) solid bolete strains are inoculated at the roots of tropical cash crops and flower plants planted in fields to culture mushroom, and fuscous dictyostelium boletes substance can be grown and developed to mature after 30-60 days of inoculation; artificial culture of fuscous dictyostelium boletes and the industrialization production of the fuscous dictyostelium boletes can be fast realized. The culture method is simple and convenient, the culture period is short and the fruiting is regular and the yield is high.

The invention discloses a method for cultivating toadstools. The method comprises the following steps that 1, compost is prepared; 2, sterilization is carried out; 3, inoculation is carried out; 4, mycelium culture is carried out, wherein culture temperature ranges from 20 DEG C to 22 DEG C, air humidity ranges from 45 percent to 55 percent, and after ten days, the temperature ranges from 15 DEG C to 17 DEG C, and the air humidity ranges from 45 percent to 50 percent; 5, land treatment is carried out; 6, a sunshade is built; 7, secondary plowing is carried out; 8, field breeding is carried out; 9, final-period management is carried out, wherein in the earlier stage, air humidity in the sunshade ranges from 30 percent to 35 percent, soil humidity ranges from 55 percent to 65 percent, and temperature ranges from 5 DEG C to 16 DEG C, after 85 days, the air humidity ranges from 83 percent to 88 percent, the soil humidity ranges from 85 percent to 90 percent, the temperature ranges from 22 DEG C to 25 DEG C, and in the fruiting process, the air humidity ranges from 60 percent to 70 percent, the soil humidity ranges from 75 percent to 80 percent, and the temperature does not exceed 28 DEG C; 10, harvesting is carried out. The method is short in period, the contamination rate of the cultivated toadstools is low, the yield is high, and quality is good.

The invention relates to a Phlebopus portentosus cultivating method. The method comprises the steps of culture medium preparing, bacterium inoculating, earthening, mycelium culture in an earthening layer and fruiting cultivating. The invention further relates to a fungus culture medium and the purpose of the fungus culture medium in Phlebopus portentosus cultivating.

The invention discloses paracoccus and the application for degrading azotic heterocyclic compound. The paracoccus is paracoccus sp. BW001 CGMCC No. 2225. The paracoccus sp. BW001 CGMCC No. 2225 of the invention, on one hand, can grow into pyridine as the only carbon source and nitrogen source, which has a strong degradation activity (the degradation rate is 100%) for pyridine, is extremely suitable for biologic control of organic wastewater (such as coking wastewater) which contains pollutant of pyridine category, and can be used as biological hardening agent in the biological control technology of organic persistent wastewater, and corresponding environmental biological agent is exploited, thereby having relatively high value of study, application and marketing.

The invention relates to a culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte, and aims to solve the problem that the prior art is low in differentiation rate. The culture method comprises the following steps: 1) performing separation of primary cells of adipose tissue-derived stromal cells; 2) performing amplification and passage of the adipose tissue-derived stromal cells; and 3) performing differentiation culture on P5-generation adipose-derived stem cells to chondrocyte, namely, adding the adipose tissue-derived stromal cells into a condition culture medium, namely, an adipose tissue-derived stromal cell chondrocyte differentiation culture medium, after P5 generation of passage, performing chondrocyte differentiation culture, replacing the medium of the cells every 3 days, observing the morphological change of the cells, and after 16 days of induction, performing Alcian blue dyeing, and identifying the chondrocyte differentiation situation of the adipose tissue-derived stromal cells. The result of Alcian blue dyeing identification shows that the chondrocyte can be formed, and the Alcian blue dyeing is positive. The culture method has the characteristics of being simple and feasible, short in induction time as the induction culture medium is a serum-free culture system, good in test repeatability and high in osteoblast differentiation rate.

The invention relates to bradyrhizobium japonicum capable of effectively fixing nitrogen and a culture method and application thereof. The bradyrhizobium japonicum CGMCC No.4346 is cultured by the steps of strain activation, seed culture, fermentation culture and the like. The invention has the following beneficial effects: sorbitol and diethylene glycol are utilized to replace the carbon source, mannitol in the traditional YEM (yeast extract mannitol) culture medium, and when the improved fermentation culture medium is adopted for culturing, the viable count of the fermentation liquor is higher than that of the YEM culture medium; compared with the rhizobium inoculants which are not inoculated on the soy beans, the bradyrhizobium japonicum agent ensures the theoretical yield of the soy beans to be increased by 16.7-17.2%, the crude fat content to be increased by 0.65-1.21% and the protein content to be increased by 1.29-1.60% after being inoculated on the soy beans, thus being an excellent strain for production of rhizobium japonicum and inoculation of the soy beans; and the culture method is simple and practical, and the raw materials have wide sources and low price and are suitable for large-scale industrialized production.

The invention belongs to the technical field of the biological pesticide and particularly relates to a biocontrol bacterium for controlling plant diseases and a preparation method thereof. The invention has the following main contents: (1) the separation and identification of Bacillussp.CC09 strain; (2) the preparation of Bacillussp.CC09 strain biocontrol microbial inoculum and the applications of the microbial inoculum in the control of plant diseases; and (3) the stability of the Bacillussp.CC09 biocontrol microbial inoculum. The Bacillussp.CC09 biocontrol microbial inoculum is characterized in that the anti-fungal spectrum is wide, the effect is long-lasting, harmful bacteria are difficult to generate drug resistance, the microbial inoculum is safe for human, livestock and crops and is environmentally friendly, etc. The biocontrol bacterium has obvious inhibitory activity for the growth of Fusariumgraminearum, Alternariaalternata, Rhizoctoniasolani and Phytophthoracapsici and especially obvious control effect on wheat powdery mildew and scab.

The invention discloses an early spring tomato seedling and greenhouse cultivation method and relates to the technical field of tomato cultivation. The method mainly comprises the following steps of (1) variety selection, (2) seedbed conditioning, (3) seed soaking and disinfection, (4) sowing and seedling raising, (5) provisional planting, (6) soil preparation and fertilization, (7) field planting, (8) field management, (9) pruning flower-reserving and fruit grading and thinning, and (10) pest control. The cultivation method provided by the invention has the advantages that the cultivation method is simple and easy to implement, is applicable to early spring tomato seedling and greenhouse cultivation, the method realizes low investment and high yield, the occurrence rate of plant diseases and insect pests is low, and the product quality and the market competitiveness are improved.

The invention provides a hypsizigus marmoreus culture medium which comprises the following components in percentage by mass: 40-50% of corn cob, 8-15% of rice bran, 3-8% of schisandra fruit dreg, 10-20% of radix salviae miltiorrhizae dreg, 5-10% of soybean hull, 12-20% of wheat bran, 0.8-2% of shell powder and 0.2-1% of lime. The invention belongs to the technical field of edible fungus culture. The hypsizigus marmoreus culture medium provided by the invention does not need wood dust or cottonseed hull, fully utilizes the schisandra fruit dreg and radix salviae miltiorrhizae dreg, and thus, has the advantages of wide raw material sources, high air permeability, favorable culture effect and the like on the premise of lowering the cost.

The invention discloses a pollution-free high-yield cultivation method for Chinese onions. The method comprises the first step of sowing and seedling raising, the second step of transplanting and management after transplanting, the third step of prevention and control of diseases and pests and the fourth step of harvest and storage of Chinese onions. The pollution-free high-yield cultivation method for Chinese onions is simple, operability is high, farmers can easily grasp the cultivation method, the yield is high, the benefit is good, and the method has extremely high application and popularization value.

The invention discloses a cultivation method for high-yield potatoes. The method includes the steps of field selection, basal dressing, seed selection, sterilization and bud forcing, sowing, field management and prevention and control of insect pests and weeds. The cultivation method has the advantages of being simple, low in cost, high in yield, convenient to manage and the like, and is suitable for large-scale cultivation.

The invention belongs to the technical field of vegetable cultivation and particularly relates to a cherry tomato cultivation method. The cherry tomato cultivation method comprises the following steps of selecting vegetable plots, erecting protective greenhouses, raising seedlings in advance, preparing lands and performing timely transplantation, field management and fruit harvesting. The vegetable plot selecting step is that selected lands are plain, convenient to irrigate and drain, good in ventilation and light transmission and loose and fertile in soil or sandy soil and are plots where the same kinds of previous-crop vegetables are not planted. The protective greenhouse erecting step is that the protective greenhouses with the height of 3-4 meters are erected, automatic water spraying devices and temperature adjusting devices are arranged in the protective greenhouses, and heat-preservation films are arranged outside greenhouses. The cherry tomato cultivation method has the advantages of being simple, free of pesticide residues, environmental-friendly, free of pollution, high in yield, convenient to manage and the like and is suitable for large-scale cultivation.

The invention relates to a cultivation method for planting summer cress in a vegetable greenhouse. The cultivation method comprises the following steps of: (1) selecting high-temperature-resistant variety; (2) forcing germination of seed stems; (3) covering with a 50-70% sunshade net; (4) plowing and finishing field surface to be flat, smooth, mashed and mature; (5) spreading and arranging the seed stems; (6) covering with rice straw; (7) tapping furrow surface with a square hoe or wood board so that the seed stems and the rice straw are coated by a layer of slurry; (8) scientifically managing and controlling fertilizer and water; (9) spraying gibberellin and additionally fertilizing at roots 4-7 days before harvesting; and (10) picking in proper stage. According to the cultivation method provided by the invention, by covering with the sunshade net, cool microclimate is built; by covering with rice straw and slurry, the survival rate of the seed stems is improved; by supplying enough fertilizer, the vegetative growth of cress is promoted; and by spraying gibberellin, the summer cultivation difficulty of cress is better solved. In addition, by adopting the cultivation method provided by the invention, water and drought crop rotation in the vegetable greenhouse is realized, and a simple and efficient new way is found for preventing and controlling continuous cropping drawback.

The invention discloses a strain of obligate halophile Y-B, which is obtained from well drilling waste mud by enrichment, screening and culture, and aims at realizing high-efficient degradation of petroleum in high-salt oily waste liquor in the petroleum industry. The strain Y-B is milky white, is of a gram-positive bacterium, is shaped like a cell rod and can produce spores, the strain Y-B is identified as Bacillus subtilis subsp. spizizenii and has very high activity, a culture method is simple, and the strain Y-B has the advantages of easiness in obtainment, low cost and rapid growth, is suitable for weakly-alkaline and high-salt environments, has a better effect of removing the petroleum in the high-salt oily waste liquor and further has broad application prospects in the treatment ofthe high-salt oily waste liquor in the petroleum industry and oil overflow sudden accidents.

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention relates to primary culture and identification methods for smooth muscle cells of esophagogastric junction via enzyme digestion method. The primary culture method includes steps of: 1) acquiring a muscle strip of smooth muscle tissue of the esophagogastric junction; 2) digesting the muscle strip of the smooth muscle tissue with a digestion liquid to prepare a smooth muscle tissue cellsuspending liquid; 3) placing the smooth muscle tissue cell suspending liquid in a culture container, in which levo-polylysine is pre-paved, and culturing the cells until adherent cells account for more than 90% of the bottom of the container or cells grow crowdedly locally. The primary culture method in the invention is simple, completed and effective. The identification method can identify thespecial phenotypes of the smooth muscle cells.