The invention belongs to the technical field of
plant leaf
punching and sampling devices, particularly relates to a puncher for accurately detecting
nucleic acid of
plant leaves and a use method, and aims to solve the problems that existing sampling devices are all provided with
metal modules and are reusable sampling devices, and single sampling device is large in size and inconvenient to use. In order to solve the problem that in the prior art, in the prior art, in the prior art, the use requirements of portability and disposable use required by
plant leaf
pathogen collection are difficult to meet, the following scheme is provided: the device comprises a
punching cylinder; the first limiting hole and the second limiting hole are formed in the outer side of the
punching barrel; according to the present invention, during the use process, the portable rapid
pathogen detection device has characteristics of portability, easy installation, simple sampling operation, convenient and rapid sampling process, consistent size of the
cut leaf tissue, regular and uniform shape, and increase of the accuracy of the
pathogen detection result, and can be used for the field
rapid detection of the field plant, the method has important significance on
quarantine, detection, monitoring and prevention and control of plant diseases and
insect pests.