30 results about "Smooth muscle tissue" patented technology

A device and method for treating bodily conduits involves the application of energy to the smooth muscle tissue of the conduit walls to reduce the bulk of smooth muscle tissue and mucus glands. The irradiation treatment of the smooth muscle tissue causes a reduction in the amount of smooth muscle tissue over time which increases the inner diameter of the body conduit for improved fluid flow and prevents smooth muscle spasms. The treatment is particularly useful in the lungs for treatment of asthma to prevent bronchospasms, increase the airway diameter for improved air exchange, and reduce mucus secretions in the lungs.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

The present invention provides a device for the stimulation of smooth muscle tissue. The device includes a stimulator arranged to provide a signal to the smooth muscle tissue to control response of the smooth muscle tissue, and an interface arranged to allow programming of a controller of the stimulator. The interface may interact with an external controller. The device finds use in controlling smooth muscle tissue, such as a neosphincter, for the control of urinary or faecal incontinence in a patient.

Methods for treating a network of organs including generating a map of at least a portion of the network of organs using a rendering system; selecting at least one treatment location within the luminal passageway of the network of organs; and applying an energy therapy to the treatment location to treat the smooth muscle tissue, where the energy therapy applied to the respective treatment location is defined by a plurality of parameters that are associated with a map. Such a system allows for historical or ideal treatment parameters to be identified, visually or otherwise to actual treatment locations. Also, control systems and methods for delivery of energy that may include control algorithms that prevent energy delivery if a fault is detected and may provide energy delivery to produce a substantially constant temperature at a delivery site. In some embodiments, the control systems and methods may be used to control the delivery of energy, such as radiofrequency energy, to body tissue, such as lung tissue.

PCT No. PCT/EP96/01043 Sec. 371 Date Oct. 27, 1997 Sec. 102(e) Date Oct. 27, 1997 PCT Filed Mar. 12, 1996 PCT Pub. No. WO96/28211 PCT Pub. Date Sep. 19, 1996A method is disclosed for treating erectile dysfunction includes arranging a therapy apparatus to be connected to an electrical power source, placing a skin electrode on two sides laterally of a penile shaft and employing an amplifier for performing the stimulation. A current is applied transcutaneously for a regeneration of the smooth musculature of the penile corpus carnaversum. The current applied has zero line-symmetrical impulses of rectangular or trapezoid form with a built-up time of 0,01 to 2 seconds, a stimulation period of 1 to 60 seconds direct or alternating, a stimulation pause of 0.01 to 60 seconds, a frequency range of 1 to 50 Hertz and an impulse period of 100 mu s to 10 ms. Advantageously, the stimulation is performed diagonally alternatingly with zero-line symmetric impulses of trapezoidal shape in a first channel and in a second channel with alternating stimulations. The frequency for the first channel is 10 to 20 Hertz and the frequency for the second channel is 20 to 35 Hertz. The impulse duration is from about 100 to 150 mu s. The rise time is 0.5 seconds, the stimulation time is 5 seconds per channel, and the pause between stimulations is 0.5 seconds. The current is increased until a sensory perception of current is reported.

The invention relates to a gastroesophageal junction smooth muscle cell tissue explant primary culture and identification method. The method includes steps: acquiring gastroesophageal junction smoothmuscle tissue muscle strips; digesting the smooth muscle tissue muscle strips by digestive juice to obtain digested smooth muscle tissue blocks; transferring the digested smooth muscle tissue blocks into a culture container to fix the digested smooth muscle tissue blocks to the bottom of the culture container, keeping a gap of 0.5-1cm between every two adjacent digested smooth muscle tissue blocks, and culturing until adherent cells occupy more than 90% of the bottom of the culture container or the adjacent digested smooth muscle tissue blocks contact through new cells. The invention further provides a smooth muscle cell identification method. The gastroesophageal junction smooth muscle cell tissue explant primary culture method is simple, perfect and effective, and specific phenotypes ofthe smooth muscle cells can be identified by adoption of the identification method.

This invention relates to a three dimensional bioengineered sphincter such as a bioengineered internal anal sphincter (IAS) and three-dimensional physiological models of smooth muscle cells. The invention also related to methods for generating a bioengineered sphincter and smooth muscle rings in culture. In addition, the invention provides for methods of using a bioengineered sphincter or bioengineered smooth muscle ring to identify agents that modify contractile force and relaxation in smooth muscle cells.

This invention relates to certain novel compounds and derivatives thereof, their synthesis, and their use as alpha 1a adrenergic receptor antagonists. One application of these compounds is in the treatment of benign prostatic hyperplasia. These compounds are selective in their ability to relax smooth muscle tissue enriched in the alpha 1a receptor subtype without at the same time inducing hypotension. One such tissue is found surrounding the urethral lining. Therefore, one utility of the instant compounds is to provide acute relief to males suffering from benign prostatic hyperplasia, by permitting less hindered urine flow. Another utility of the instant compounds is provided by combination with a human 5-alpha reductase inhibitory compound, such that both acute and chronic relief from the effects of benign prostatic hyperplasia are achieved.

The invention provides medicine target protein of serums after a Yimusake tablet acts on rats with an abnormal balham syndrome and an abnormal Balham type erectile dysfunction disease, and a penis smooth muscle tissue, and a screening method thereof. The method comprises the following steps of 1, providing a normal comparison set, a syndrome set, a syndrome medicine set, a disease set and a disease medicine set; 2, collecting whole blood, separating the serum and the penis smooth muscle tissue from the whole blood; 3, extracting the protein; 4, purifying after marking; 5, acquiring a fingerprint spectrum after spectrum detection, analyzing protein compositions after screening, and acquiring each set of serums and the penis smooth muscle tissue protein; and 6, acquiring a Yimusake tablet action target protein system by making statistics, comparing and screening. According to the protein and the screening method of the protein provided by the invention, the action target of the uigur medicine, the Yimusake tablet, on the abnormal Balham type erectile dysfunction in uigur medicine is clear, and a foundation is laid for further disclosure of the action mechanism of the Yimusake tablet and new and secondary development of the Yimusake tablet.

The invention provides a mammalian differential serum protein and a differentially expressed protein system of the penile smooth muscle tissue of an abnormal and phlegmatic type of impotence syndrome in uighur medicine, and a screening method of the differentially expressed protein system. The method comprises the steps that 1, a normal control group is formed, a model of a syndrome group and a disease syndrome group is built; 2, whole blood is collected, the serum and the penile smooth muscle tissue are separated; 3, protein is extracted; 4, after the labeling, purification is conducted; 5, after the detection of mass spectrum, a fingerprint spectrum is obtained, proteomics analysis is conducted after the screening, and the protein of serum and the protein of penile smooth muscle tissue of the syndrome group, the disease syndrome group and the normal control group are obtained; 6, differentially expressed protein of serum and differentially expressed protein of penile smooth muscle tissue of abnormal and phlegmatic type of impotence syndrome in uighur medicine are obtained by statistic measurements, comparisons and screening, and a foundation and a reference are provided for further mechanism research, medicine screening, conversion, and prevention and cure research on abnormal and phlegmatic type of impotence syndrome.

The digestion process of smooth muscle of blood vessels belongs to the field of biomedical technology. The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally. The process uses less enzyme varieties, is low in cost and simple, and may be used in medicine, biology and other fields.

The present invention relates to a method and apparatus for the treatment of a heart condition such as angina and/or congestive heart failure. Conditions such as angina, caused by inadequate blood flow to the coronary arteries, are treated in this invention by implantation of smooth muscle tissue wrapped around the ascending aorta. The smooth muscle tissue is stimulated electrically, by the implanted stimulator, to contract at timed intervals relative to the hearts' cycle. The contraction assists blood flow to the coronary vessels.

The present disclosure relates to methods for relaxing airway smooth muscle tissue, alleviating or preventing bronchospasm, and treating lung diseases by administering an HMG-CoA reductase inhibitor (statin) directly to lung tissue by inhalation. The disclosure also relates to formulations and compositions useful for the practice of methods of the disclosure.

The invention discloses a construction method of an animal ureteral calculus model. The method comprises the following steps of selecting a white rabbit, and performing ear vein injection by using a urethane solution; enabling the rabbit to be on an operating table with the supine position, enabling the lower left abdomen to be subjected to hair scraping, disinfecting the skin through iodophor, performing incision on the left side of the middle of the abdomen, and entering the abdominal cavity layer by layer; after entering the abdominal cavity, dissociating the paracolic sulci at the left side, pushing open the descending colon and the mesostenium to expose the ureter at the left side, injecting flowing resin into the upper end of the dissociating ureter by using an injector, and irradiating and fixing by using a photocuring lamp; resetting the colon at the left side and the mesostenium after no urine leakage occurs, closing the abdominal cavity layer by layer, disinfecting the woundwith iodophor, and covering the wound with gauze; and three days after the operation, performing penicillin U intramuscular injection every day. According to the animal ureteral calculus model constructed by the method, the flowing resin material has the characteristics of short solidification time, small damage to smooth muscle tissues and the like, and the model has the advantages of good calculus forming effect, short calculus forming time, economy, timeliness, reproducibility, high controllability and the like.

The invention belongs to the technical field of medical instrument surgical implants, and particularly relates to a bionic three-layer artificial blood vessel and a preparation method thereof. The artificial blood vessel is of a three-layer composite structure and sequentially comprises a polyurethane tubular film, an artificial blood vessel reinforcing body and an outer wall coating from inside to outside, polyurethane is selected as a blood vessel preparation raw material for the inner layer, and the polyurethane material is good in biocompatibility and excellent in elasticity, can guarantee smooth blood flowing and reduce thrombus generation and has good weather resistance; the service life is long; the middle layer is made of tubular fabric, is a smooth muscle tissue imitating the blood vessel middle layer, and plays a role in increasing the support of the blood vessel prosthesis and increasing the strength; the outer wall coating simulates blood vessel outer wall connective tissue, and invasion and growth of tissue cells are facilitated. The artificial blood vessel provided by the invention has good biocompatibility and high impermeability, and the success rate of an operation can be improved.

The invention relates to the technical field of medical tissue engineering, in particular to a new born child tissue engineering bladder piece and a preparation method thereof. The new born child tissue engineering bladder piece provided by the invention is characterized by comprising smooth muscle-like cells formed by differentiation and a degradable scaffold material; the smooth muscle-like cells wrap the degradable scaffold material and are flaky; and the smooth muscle-like cells express alpha smooth muscle actin (alpha-SMA), calponin (CALP) and major histocompatibility complex (MHC). In terms of mechanical indexes, the new born child tissue engineering bladder piece has similar mechanical tensile characteristic with normal bladder smooth muscle tissues, which helps to restore the consistency of the bladder compliance during rebuilding of filling of bladder.

Methods and compositions are provided for regenerating smooth muscle tissue by the provision of a purified population of epicardial-derived pericytes, where the smooth muscle tissue may comprise, without limitation, coronary artery tissue; kidney tissue, etc. Compositions and kits for practicing the methods and/or for use with the systems of the disclosure are also provided.