30 results about "Smooth muscle tissue" patented technology

The invention relates to primary culture and identification methods for smooth muscle cells of esophagogastric junction via enzyme digestion method. The primary culture method includes steps of: 1) acquiring a muscle strip of smooth muscle tissue of the esophagogastric junction; 2) digesting the muscle strip of the smooth muscle tissue with a digestion liquid to prepare a smooth muscle tissue cellsuspending liquid; 3) placing the smooth muscle tissue cell suspending liquid in a culture container, in which levo-polylysine is pre-paved, and culturing the cells until adherent cells account for more than 90% of the bottom of the container or cells grow crowdedly locally. The primary culture method in the invention is simple, completed and effective. The identification method can identify thespecial phenotypes of the smooth muscle cells.

The invention relates to a gastroesophageal junction smooth muscle cell tissue explant primary culture and identification method. The method includes steps: acquiring gastroesophageal junction smoothmuscle tissue muscle strips; digesting the smooth muscle tissue muscle strips by digestive juice to obtain digested smooth muscle tissue blocks; transferring the digested smooth muscle tissue blocks into a culture container to fix the digested smooth muscle tissue blocks to the bottom of the culture container, keeping a gap of 0.5-1cm between every two adjacent digested smooth muscle tissue blocks, and culturing until adherent cells occupy more than 90% of the bottom of the culture container or the adjacent digested smooth muscle tissue blocks contact through new cells. The invention further provides a smooth muscle cell identification method. The gastroesophageal junction smooth muscle cell tissue explant primary culture method is simple, perfect and effective, and specific phenotypes ofthe smooth muscle cells can be identified by adoption of the identification method.

The invention provides a mammalian differential serum protein and a differentially expressed protein system of the penile smooth muscle tissue of an abnormal and phlegmatic type of impotence syndrome in uighur medicine, and a screening method of the differentially expressed protein system. The method comprises the steps that 1, a normal control group is formed, a model of a syndrome group and a disease syndrome group is built; 2, whole blood is collected, the serum and the penile smooth muscle tissue are separated; 3, protein is extracted; 4, after the labeling, purification is conducted; 5, after the detection of mass spectrum, a fingerprint spectrum is obtained, proteomics analysis is conducted after the screening, and the protein of serum and the protein of penile smooth muscle tissue of the syndrome group, the disease syndrome group and the normal control group are obtained; 6, differentially expressed protein of serum and differentially expressed protein of penile smooth muscle tissue of abnormal and phlegmatic type of impotence syndrome in uighur medicine are obtained by statistic measurements, comparisons and screening, and a foundation and a reference are provided for further mechanism research, medicine screening, conversion, and prevention and cure research on abnormal and phlegmatic type of impotence syndrome.