A differential protein system, screening method and application of a mammalian serum and penile smooth muscle tissue with abnormal mucus type impotence syndrome

Abstract

The invention provides a mammalian differential serum protein and a differentially expressed protein system of the penile smooth muscle tissue of an abnormal and phlegmatic type of impotence syndrome in uighur medicine, and a screening method of the differentially expressed protein system. The method comprises the steps that 1, a normal control group is formed, a model of a syndrome group and a disease syndrome group is built; 2, whole blood is collected, the serum and the penile smooth muscle tissue are separated; 3, protein is extracted; 4, after the labeling, purification is conducted; 5, after the detection of mass spectrum, a fingerprint spectrum is obtained, proteomics analysis is conducted after the screening, and the protein of serum and the protein of penile smooth muscle tissue of the syndrome group, the disease syndrome group and the normal control group are obtained; 6, differentially expressed protein of serum and differentially expressed protein of penile smooth muscle tissue of abnormal and phlegmatic type of impotence syndrome in uighur medicine are obtained by statistic measurements, comparisons and screening, and a foundation and a reference are provided for further mechanism research, medicine screening, conversion, and prevention and cure research on abnormal and phlegmatic type of impotence syndrome.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mammalian serum and penile smooth muscle tissue differential protein of abnormal mucus type impotence syndrome of Uighur medicine, a screening method and application thereof. Background technique [0002] Uighur medicine (Uyghur medicine) is an important part of the traditional medicine of the motherland. Uighur medicine humor theory believes that the human body is composed of 4 kinds of humors: biliary, bloody, phlegmatic and black gallbladder. The dynamics of the above four kinds of humoral Balance is the physiological basis of a healthy body, and a change in the quantity or / and quality of a certain body fluid quality will cause the imbalance of body fluid quality and produce an abnormal body fluid quality syndrome (syndrome), which has a causal relationship with the occurrence of various diseases. Humoral pathology (humoral theory) is the basis of Uygur medicine theo...

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Problems solved by technology

In the direction of clinical and basic research of Uyghur medicine, due to the late start of experimental zoology, there is no combination of related diseases and syndromes with animal models, resulting in abnormal mucus type impotence syndrome and the research on the mechanism of the corresponding prescriptions and medicines has been lagging behind, and severely restricted Uyghur medicine. In the research and development of andrology in this field, there is no research report on the related marker proteins and protein marker systems of abnormal mucus type impotence syndrome

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