32 results about "Tissue protein" patented technology

The invention discloses a large model and multi-mode fused lysine modification site prediction method and device, relates to the technical field of biological information, and mainly aims to solve the problem of poor prediction effectiveness of lysine modification sites in existing proteins. Comprising the following steps: acquiring amino acid sequence information of cell tissue protein, and processing the amino acid sequence information based on a protein large language model which completes model training to obtain sequence representation; obtaining amino acid atom information of the cell tissue protein, and processing an amino acid contact graph structure constructed based on the amino acid atom coordinate information based on a graph structure model of which model training is completed to obtain structural characterization; and splicing the sequence representation and the structure representation, and performing feature fusion prediction on a representation vector obtained after splicing based on a multi-modal fusion model of which model training is completed to obtain a lysine modification site prediction result.

The invention provides a programmable hydrogel wound dressing with rotation contraction force and application thereof, and belongs to the technical field of biomedical materials, the programmable hydrogel wound dressing comprises a dual-network hydrogel main body, a first hydrogel layer, a second hydrogel layer, a first hydrogel layer and a second hydrogel layer, and the dual-network hydrogel main body is composed of a first network and a second network; the first network is a chemically cross-linked methacrylated hyaluronic acid network and forms a cross-linked network structure through photopolymerization; the second network is a physical cross-linked polyvinyl alcohol network, and forms an instantaneous cross-linking point with a crystal region through a hydrogen bond, so that the hydrogel is endowed with shape memory performance; the surface is modified with an N-hydroxysuccinimide ester functional group, and the N-hydroxysuccinimide ester functional group and amino of wound tissue protein can form a covalent bond; the dual-network hydrogel main body is subjected to pre-stretching or pre-twisting treatment and then is dried and shaped into a preset shape, the initial shape is recovered after moisture is absorbed, and rotary contraction mechanical force is output. Rapid wound healing and scar-free regeneration are achieved by dynamically regulating and controlling wound edge mechanical force and enhancing tissue adhesion, and then application to regulation and control of wound microenvironment to promote scar-free healing is achieved.

The invention belongs to the technical field of biological pharmacy, and provides an application of jonquil glucoside in preparation of a medicine for treating a metabolism-related fatty liver disease, and the application is that the jonquil glucoside is applied to preparation of the medicine for treating the metabolism-related fatty liver disease. The treatment effect of the jonquil glycoside on MASLD is evaluated by adopting a method for establishing a fatty liver model through induction of HFD high-fat, high-fructose and high-cholesterol feed. Results show that the jonquil glucoside can reduce the liver weight by inhibiting weight gain, reduce the content of TC and TG in serum, improve the conditions of liver inflammation and fat change, reduce the oxidative stress level of liver tissue and restore SIRT1 protein expression of the liver tissue to achieve the purpose of treating MASLD.

The invention provides a sealing material for wet tissues as well as a preparation method and application of the sealing material. The sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres, the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microsphere is of a core-shell structure, and the core-shell structure comprises an inner core and an outer shell wrapping the inner core; the inner core comprises a temperature-sensitive cross-linked network and polysaccharide capable of being subjected to ionic cross-linking, and the shell is a porous shell body network and is formed by carrying out copolymerization reaction on a natural polymer derivative capable of being subjected to photo-crosslinking and a comonomer containing an active chemical group. The temperature-sensitive microspheres with core-shell structures are designed to cooperate with the hydrophobic oil phase, rapid hemostasis of dynamic and bleeding wet tissues is realized through a dual drainage mechanism of instantaneous drainage of the hydrophobic oil phase and active pumping of the temperature-sensitive inner core, and then stable chemical bonding is formed through cross-linking of the shell and tissue protein, so that the rapid hemostasis of the dynamic and bleeding wet tissues is realized. And the wet tissue is sealed.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for improving a cognitive function as well as a preparation method, a product and application of the composition. The composition is prepared by taking soybean meal, whey protein peptide powder and bovine bone collagen peptide powder in a mass ratio of 1: 3: 2 as peptide source raw materials, matching the peptide source raw materials with AMY001, lactobacillus plantarum LP-90 and clostridium butyricum (3: 1: 1) compound strains, and carrying out enzyme production self-hydrolysis by bacillus amyloliquefaciens, peptide-bacterium anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. According to the present invention, the hippocampus Abeta1-42 protein can be cleared, the BDNF content can be increased, the IL-6 expression inhibition multi-mechanism synergistic effect can be achieved, the memory improvement rate and the Abeta1-42 clearance rate are high, and the cognitive impairment can be significantly repaired. The composition is high in stability and bioavailability, controllable in preparation process and suitable for development of food and health care products for improving the cognitive function and medicines for preventing or assisting in improving diseases related to cognitive impairment.

The application provides a kind of based on digestive tract tissue protein expression abundance determination method and system, applied to digestive tract tissue image processing technical field. Including: based on the PVDF membrane image after color development of digestive tract tissue acquisition in vitro, contain at least one protein standard band and the protein band of multiple target proteins;When determining the presence of electrophoresis distortion according to the band of target protein standard, construct pixel space coordinate system;The target image is obtained by angle correction and linear repair to PVDF membrane image, and the protein band corresponding to multiple target proteins in target image is in horizontal alignment state under pixel space coordinate system;Determine the development area of each target protein in target image and the irregular region of interest wrapped the protein band corresponding to multiple target proteins, and for each target protein, the expression abundance of target protein is obtained by analyzing the development area and the irregular region of interest, and the accuracy of the protein expression abundance is higher.

The invention relates to a compound feed for promoting growth and development of meat pigeons. The compound feed comprises the following raw material components: corn flour, wheat flour, vegetable oil, an auxiliary agent and a beef tallow modified additive, wherein the beef tallow modified additive is a product obtained by blending beef tallow and amino acid, then carrying out ultrasonic heating on the blended beef tallow and amino acid and diatomite, and then carrying out a reaction. The beef tallow contains a large amount of fatty acid and can react with surface active hydroxyl groups of the diatomite after being mixed with the amino acid, the beef tallow and the amino acid can be loaded by the abundant porous structure of the diatomite, and the beef tallow, the amino acid and the beef tallow form a stable compound, so that harmful substances such as mould, bacteria and excessive ammonia gas are adsorbed by the diatomite; according to the method, the intestinal burden of the meat pigeons and the risk of intestinal inflammation are relieved, more nutrients and active substances are provided, and the absorption effect on corn flour and wheat flour is promoted, so that the absorption efficiency is improved, and the requirement of tissue protein synthesis is more accurately met.

The utility model belongs to the technical field of textured protein manufacturing molds, and particularly relates to a multipurpose textured protein manufacturing mold which comprises a mounting box and a demolding assembly arranged on the outer side of the end of the mounting box. The demolding assembly comprises a shaping box, partition plates and sliding holes, the outer sides of the ends of the multiple mounting boxes are fixedly connected with the shaping box, and the inner sides, provided with the rectangular grooves, of the shaping box are evenly and fixedly connected with the multiple partition plates; the first sliding column is pressed to drive the second sliding column to move towards the third sliding column, then the ejection plate is ejected out, demolding can be achieved through simple pressing operation, the operation process is greatly simplified, the time and labor cost needed by demolding are reduced, and therefore the overall production efficiency is improved, and the production cost is reduced. According to the demolding device, the design that demolding is conducted on the designated position singly is achieved through the different first sliding columns, product damage or deformation possibly caused by overall demolding is avoided, the completeness and quality of products can be guaranteed through the accurate demolding mode, and the defective product rate is reduced.

The present invention discloses an application and a kit for differential histoproteins and / or metabolites of pigmented villonodular synovitis; the differential histoproteins include at least one of the following proteins: TNFSF11, cathepsin K, and adhesion G protein-coupled receptor E5; the differential metabolites include at least one of the following products: 13-hydroxyperoxylinoleic acid and octadecadienoic acid; the present invention provides a new screening marker for pigmented villonodular synovitis (PVNS) through proteomics and metabolomics, which can achieve effective screening for pigmented villonodular synovitis; the obtained screening marker is used in a screening kit, which only needs synovial fluid as a test sample, can quickly perform screening and detection, and has the advantages of high efficiency and simple detection.

The application provides a programmable hydrogel wound dressing with rotary contractive force and application thereof, and belongs to the technical field of biomedical materials, and comprises: a double-network hydrogel main body composed of a first network and a second network; the first network is a chemically cross-linked methacrylated hyaluronic acid network, and the cross-linked network structure is formed through photo polymerization; the second network is a physically cross-linked polyvinyl alcohol network, and the instantaneous cross-linking points are formed through hydrogen bonds and crystal regions, and the hydrogel is endowed with shape memory performance; the surface is modified with N-hydroxysuccinimide ester functional groups, which can form covalent bonds with the amino groups of tissue proteins on a wound surface; the double-network hydrogel main body is dried and shaped into a preset shape after being pre-stretched or pre-twisted, restores the initial shape after absorbing moisture, and outputs a rotary contractive mechanical force. The application realizes rapid wound healing and scar-free regeneration by dynamically regulating the mechanical force at the wound edge and enhancing tissue adhesion, and further realizes the application in regulating the wound microenvironment to promote scar-free healing.

The invention provides tissue protein using soybean processing byproducts as raw materials and a preparation method of the tissue protein, and belongs to the technical field of food processing. The tissue protein is prepared from the following raw materials in parts by weight: 8 parts of soybean protein isolate, 4 parts of vital gluten, 8 parts of fresh and wet bean dregs and 7 parts of yellow serofluid, the fresh and wet bean dregs are obtained by soaking raw material dry soybeans in water, grinding into thick liquid and filtering, the rest product is soybean milk, and the yellow serofluid is a liquid byproduct collected in the bean curd pressing process. The bean curd is prepared by taking the soybean milk as a raw material. According to the method, after the soybean byproducts and the raw materials for preparing the tissue protein are treated by the single screw extruder, harmlessness is completely realized, and the nutritional value and functional characteristics of the tissue protein are favorably improved.

The invention discloses application of K224 lactic acid modification of HNRNPF as a target spot in preparation of a product for diagnosis and / or treatment of pancreatic cancer, and belongs to the technical field of tumor precision medical products. The embodiment of the invention proves that the lactylation modification level of the K224th site of the HNRNPF protein in pancreatic cancer tissues is obviously higher than that of para-carcinoma tissues, the lactylation modification level of the 224th lysine of the HNRNPF protein is positively related to the progression of pancreatic cancer, and the multiplication capacity of pancreatic cancer cells can be obviously inhibited by constructing the HNRNPF K224R site mutant. On the basis, the reagent for detecting the HNRNPF K224 lactylation level can be used for preparing products for diagnosing and / or treating pancreatic cancer, such as a reagent for detecting or inhibiting the modification and the like. The application provides a novel specific marker and an intervention strategy for early diagnosis and targeted therapy of pancreatic cancer, and has important significance and application value.

The present application relates to a method for producing fermented plant tissue protein with enhanced adhesiveness. According to the present application, the adhesiveness of the plant tissue protein is improved by the mold mycelium formed between the plant tissue protein (TVP) particles and the squeezing process, and in one embodiment, a plant tissue protein having a soft texture similar to that of meat tissue can be produced.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

This application discloses the application of HNRNPF K224 lactation modification as a target in the preparation of products for the diagnosis and / or treatment of pancreatic cancer, belonging to the field of precision oncology product technology. Examples of this application demonstrate that the lactation modification level of HNRNPF protein at site K224 is significantly higher in pancreatic cancer tissue than in adjacent normal tissue. The lactation modification level of lysine 224 in HNRNPF protein is positively correlated with the progression of pancreatic cancer. Constructing the HNRNPF K224R point mutant can significantly inhibit the proliferation of pancreatic cancer cells. Based on this, this application provides reagents for detecting HNRNPF K224 lactation levels, which can be used to prepare products for the diagnosis and / or treatment of pancreatic cancer, such as reagents for detecting or inhibiting this modification. This application provides new specific biomarkers and intervention strategies for the early diagnosis and targeted therapy of pancreatic cancer, and has significant meaning and application value.

The invention provides a method for preparing tissue protein through ultrasonic treatment of soybean protein isolate. The method comprises the following steps: weighing a certain amount of soybean protein isolate, preparing a solution with the mass concentration of 7% by using deionized water, uniformly stirring, and standing overnight; then putting the soybean protein isolate into an ultrasonic treatment machine, and treating for 5-20 minutes under the condition that the ultrasonic power is 300-1000W, so as to obtain the soybean protein isolate subjected to ultrasonic treatment; mixing the soybean protein isolate with vital gluten, soybean protein concentrate and corn starch according to a weight ratio of 4: 2: 3: 1 to obtain a mixture, adding the mixture into a twin-screw extruder for extrusion, and setting parameters such as barrel temperature, feeding rate, screw rotation speed and the like of the extruder to obtain high-moisture textured protein and low-moisture textured protein, so as to obtain the high-moisture textured protein and low-moisture textured protein. The method disclosed by the invention reveals the effect of ultrasonic treatment in tissue protein modification, enriches the tissue protein processing theory, improves the quality of the tissue protein, and broadens the application of the soybean protein isolate in the food industry.

The application provides a sealing material for wet tissues, a preparation method and application thereof, and the sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres; the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microspheres have a core-shell structure, the core-shell structure comprises a core and a shell covering the core; the core comprises a temperature-sensitive cross-linked network and an ion-cross-linkable polysaccharide, and the shell is a porous shell body network and is formed by a copolymerization reaction of a light-cross-linkable natural polymer derivative and a co-monomer containing an active chemical group. The application realizes rapid hemostasis on dynamic and bleeding wet tissues through a double drainage mechanism of the hydrophobic oil phase instantaneously draining liquid and the temperature-sensitive core actively pumping, and realizes sealing on the wet tissues through the shell and tissue protein cross-linking to form stable chemical adhesion.

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

The invention is suitable for the technical field of veterinary drugs, and provides a preparation for salpingitis of laying hens and a preparation method thereof.The preparation is prepared from thyme essential oil, modified resveratrol, modified chitosan, sodium alginate-zinc ion gel microspheres, modified epigallocatechin gallate and mussel mucin. According to the invention, the sodium alginate-zinc ion gel microspheres are intelligently swelled in an acidic inflammation environment of the fallopian tube, and zinc ions are released to inhibit a pathogenic bacteria biological membrane; the zinc oxide nanorods directionally arranged in the magnetic field enhance the tissue permeability and improve the drug concentration of the focus; mussel mucoprotein is covalently crosslinked with tissue protein through catechol groups, so that the residence time is prolonged; activated amino groups of the modified chitosan are combined with negative charges of mucous membranes to form a physical barrier to block pathogen adsorption; the thyme essential oil destroys bacterial cell membranes, so that the permeation efficiency of subsequent antibacterial components is improved; the free radical scavenging capacity of the modified resveratrol-quercetin eutectic is improved compared with that of a monomer.

The application discloses a lactobacillus gasseri for repairing a vaginal barrier and relieving a vaginal infection, and a postbiotic thereof, and belongs to the technical field of microorganisms.The lactobacillus gasseri and the postbiotic thereof can repair a vaginal barrier and relieve a vaginal infection when being used externally.The lactobacillus gasseri is separated from vaginal secretion of a healthy female, has the functions of repairing a vaginal mucosa barrier and relieving a vaginal infection, and specifically has the following effects: improving a cell monolayer barrier transendothelial electrical resistance value, increasing integrity, reducing FITC-labeled dextran permeability of the cell monolayer barrier, and reducing permeability;adjusting a mouse vaginal mucin level;improving a mouse vaginal tissue protein expression level;reducing mouse vaginal tissue IL-1 beta secretion and myeloperoxidase content;and improving a mouse vaginal tissue pathological condition.Therefore, the lactobacillus gasseri has great application prospects in products for repairing a vaginal mucosa barrier and relieving a vaginal infection caused by pathogenic bacteria.

The present application belongs to the technical field of biological medicine, and particularly relates to a composition for improving cognitive function, a preparation method, products and applications thereof. The composition is prepared from soybean meal powder, whey protein peptide powder and bovine collagen peptide powder in a mass ratio of 1:3:2 as a peptide source raw material, in combination with AMY001, Lactobacillus plantarum LP-90 and Clostridium butyricum (3:1:1) composite strains, through Bacillus amyloliquefaciens enzyme production self-digestion, peptide-bacteria anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. The composition can work synergistically through multiple mechanisms such as clearing hippocampal Aβ1-42 protein, increasing BDNF content and inhibiting IL-6 expression, has high memory improvement rate and Aβ1-42 clearance rate, and can significantly repair cognitive impairment. The composition has high stability and bioavailability, the preparation process is controllable, and is suitable for the development of drugs for preventing or assisting in improving cognitive impairment related diseases.

The utility model discloses a vein stripping hemostasis device which comprises a catheter, one end of the catheter is fixedly connected with a guide head, the surface of the catheter is provided with an embedding groove, a wire is arranged in the embedding groove, one side of the wire is fixedly connected with an electrode, the surface of the guide head is provided with a containing groove, and the electrode is fixedly connected with the containing groove. The electrode is located in the containing groove, a connecting base is movably arranged on the surface of the guide pipe, a power line is fixedly connected to the surface of the connecting base, and the wire extends into the connecting base and is electrically connected with the power line. The guide head is arranged to drive the catheter to enter a tunnel generated in a vein stripping operation, the connecting seat is moved to drive the electrode to extend out of the containing groove, and the electrode is arranged to make contact with a bleeding point and transmit energy, so that tissue protein at the bleeding point is solidified, and the aim of stopping bleeding is achieved. Hematoma can be avoided.

The application discloses a biological macromolecular drug for tumor treatment, which has tissue protein adhesion, can continuously improve the oxidative stress environment of a tumor site by prolonging the time at the tumor site, can inhibit tumor growth by tumor microenvironment regulation, can also be used as an auxiliary therapeutic agent to enhance the curative effect of other tumor treatments, and has a good clinical transformation prospect.

This invention discloses a plant-based meat block and its preparation method, belonging to the field of food processing. The plant-based meat block, by weight, is composed of 35-45 parts water, 25-35 parts textured protein, 8-13 parts egg white powder, 5-11 parts vegetable oil, 5-8 parts sugar, 4-7.5 parts TG enzyme, 1.5-2 parts gel-type soy protein isolate obtained from high-oleic soybeans, and seasonings. The preparation method includes steps such as soaking textured protein, frying at 165-175℃ for 6-8 min, braising at 55-65℃ for 30-40 min, mixing, settling at 3-5℃, steaming at 120℃ for 50 min, and sterilization. This invention, through a reasonable ratio of egg white powder and soy protein isolate and the introduction of TG enzyme for cross-linking, reduces the amount of exogenous additives. The resulting product has a firm structure, is not easily broken when cut, has a high similarity in taste to meat, and the processing technology is simple, facilitating industrial production.

The utility model relates to the technical field of textured protein processing, in particular to a textured protein splitting and dewatering device which comprises a fixing frame, a noise reduction assembly is arranged at the lower end of the fixing frame, and the noise reduction assembly comprises a first connecting plate, a damper, a mounting base, a spring and a second connecting plate. By arranging the damper and the spring, a good noise reduction effect can be achieved for the device, when tissue protein is subjected to thread removal and dehydration work, high-speed rotating mechanical motion enables the device to vibrate so as to make noise, and when the device operates, the vibration generated by the mechanical motion can be transmitted to the first connecting plate, so that the device is prevented from being damaged. The first connecting plate absorbs vibration through the damper and the spring, the damper can reduce the vibration amplitude, and the damper is matched with the spring, so that the noise reduction assembly can effectively reduce noise generated during operation of the device, a more comfortable working environment is created for operators, meanwhile, the device can be protected against damage caused by vibration, and the service life of the device is prolonged.

The invention relates to a microfluidic system for rapid quantitative detection of breast cancer tissue markers and a detection method, and belongs to the technical field of biology, the microfluidic system comprises an acrylic cover plate, a pressure-sensitive adhesive layer and a glass substrate; the acrylic cover plate, the pressure-sensitive adhesive layer and the glass substrate are bonded together from top to bottom, and the reaction cavity of the micro pressure-sensitive adhesive layer is filled with antibody microbeads. And the micro reaction cavity, the inlet channel and the outlet channel of the pressure-sensitive adhesive layer form a micro flow path structure. According to the invention, quantification and standardization of the whole process from a tissue sample to a molecular typing result are realized, and the qualitative / semi-quantitative dependence problem is solved; an innovative tissue protein rapid preparation method, a special microfluidic system and a data analysis process based on fluorescence quantification are integrated into a whole; the whole process thoroughly gets rid of dependence on traditional tissue slicing, dyeing and manual microscopic examination.

Described herein are compositions comprising viral vectors. The viral vectors can encode a tubulin organizing protein or peptide, such as a cardiac isoform of bridging integrator 1 (cBIN1). Also disclosed herein are methods of treating or preventing heart failure in a subject in need thereof. The methods of treatment or prevention can comprise administering a vector comprising cBIN1 to a subject who has experienced heart failure or who has chronic myocardial stress, to repair or enhance contractile (systolic) function or relaxation (diastolic) function in the subject's heart.

The present invention addresses the problem of providing a textured protein material that can be used as a meat substitute in a raw material for the production of processed foods, or as a meat substitute product as is, and has improved flavor in comparison with conventional meat substitutes.　Provided is a production method for a flavored textured protein material in which a texturized raw material containing a protein raw material is texturized by means of introducing said texturized raw material into an extruder, and subjecting the same to kneading, pressing, and heating, said textured protein material production method being characterized in that a powdered protein raw material flavored by being brought into contact with a flavoring gas in advance is added so as to be least 3 wt% of the protein raw material.

The invention relates to the field of pharmacodynamic evaluation methods of traditional Chinese medicine compounds, and particularly discloses a pharmacodynamic evaluation method of traditional Chinese medicine composition intervened diabetic nephropathy, which comprises the following steps: taking diabetic nephropathy mouse models as a model group, taking diabetic nephropathy mouse models treated by traditional Chinese medicine composition as a traditional Chinese medicine composition group, and taking healthy mice as a normal control group; key differential expression proteins of kidney tissues of the mice with the diabetic nephropathy in the model group, the traditional Chinese medicine composition group and the normal control group are analyzed, principal component analysis is carried out by utilizing the key differential expression protein combination of the kidney tissues with the diabetic nephropathy, and the traditional Chinese medicine composition group is close to the normal control group and far away from the model group. The kidney tissue protein profile is converted to a normal state, which indicates that the traditional Chinese medicine composition has the efficacy of actively regulating the pathological state of the kidney tissue of the mouse with the diabetic nephropathy, and the traditional Chinese medicine composition is radix astragali and cassia twig five-component decoction. The method provided by the invention can be used for evaluating the efficacy of the traditional Chinese medicine composition intervening in the diabetic nephropathy.

The invention relates to the technical field of medicines, and provides application of miRNA-3614-5p in preparation of a medicine for treating sepsis lung injury. An in-vitro cell experiment shows that the miRNA-3614-5p inhibitor is used for treating the vascular endothelial cells, so that the functions of the vascular endothelial cells under the sepsis condition can be saved, including improving the barrier function of the vascular endothelial cells and reducing the permeability of the vascular endothelial cells; the proliferation function is improved; the mononuclear cell adhesion is reduced; and the like. In-vivo animal experiments show that treatment with the miRNA-3614-5p inhibitor can improve the pathological structure of sepsis lung injury, improve pulmonary edema and tissue protein exudation, and improve the pulmonary vessel barrier ability. Therefore, the invention provides a new basis for the miRNA-3614-5p and the inhibitor thereof to promote the repair of the sepsis lung injury.