18 results about "Tissue protein" patented technology

The invention belongs to the technical field of biological pharmacy, and provides an application of jonquil glucoside in preparation of a medicine for treating a metabolism-related fatty liver disease, and the application is that the jonquil glucoside is applied to preparation of the medicine for treating the metabolism-related fatty liver disease. The treatment effect of the jonquil glycoside on MASLD is evaluated by adopting a method for establishing a fatty liver model through induction of HFD high-fat, high-fructose and high-cholesterol feed. Results show that the jonquil glucoside can reduce the liver weight by inhibiting weight gain, reduce the content of TC and TG in serum, improve the conditions of liver inflammation and fat change, reduce the oxidative stress level of liver tissue and restore SIRT1 protein expression of the liver tissue to achieve the purpose of treating MASLD.

The invention provides a sealing material for wet tissues as well as a preparation method and application of the sealing material. The sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres, the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microsphere is of a core-shell structure, and the core-shell structure comprises an inner core and an outer shell wrapping the inner core; the inner core comprises a temperature-sensitive cross-linked network and polysaccharide capable of being subjected to ionic cross-linking, and the shell is a porous shell body network and is formed by carrying out copolymerization reaction on a natural polymer derivative capable of being subjected to photo-crosslinking and a comonomer containing an active chemical group. The temperature-sensitive microspheres with core-shell structures are designed to cooperate with the hydrophobic oil phase, rapid hemostasis of dynamic and bleeding wet tissues is realized through a dual drainage mechanism of instantaneous drainage of the hydrophobic oil phase and active pumping of the temperature-sensitive inner core, and then stable chemical bonding is formed through cross-linking of the shell and tissue protein, so that the rapid hemostasis of the dynamic and bleeding wet tissues is realized. And the wet tissue is sealed.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for improving a cognitive function as well as a preparation method, a product and application of the composition. The composition is prepared by taking soybean meal, whey protein peptide powder and bovine bone collagen peptide powder in a mass ratio of 1: 3: 2 as peptide source raw materials, matching the peptide source raw materials with AMY001, lactobacillus plantarum LP-90 and clostridium butyricum (3: 1: 1) compound strains, and carrying out enzyme production self-hydrolysis by bacillus amyloliquefaciens, peptide-bacterium anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. According to the present invention, the hippocampus Abeta1-42 protein can be cleared, the BDNF content can be increased, the IL-6 expression inhibition multi-mechanism synergistic effect can be achieved, the memory improvement rate and the Abeta1-42 clearance rate are high, and the cognitive impairment can be significantly repaired. The composition is high in stability and bioavailability, controllable in preparation process and suitable for development of food and health care products for improving the cognitive function and medicines for preventing or assisting in improving diseases related to cognitive impairment.

The application provides a kind of based on digestive tract tissue protein expression abundance determination method and system, applied to digestive tract tissue image processing technical field. Including: based on the PVDF membrane image after color development of digestive tract tissue acquisition in vitro, contain at least one protein standard band and the protein band of multiple target proteins;When determining the presence of electrophoresis distortion according to the band of target protein standard, construct pixel space coordinate system;The target image is obtained by angle correction and linear repair to PVDF membrane image, and the protein band corresponding to multiple target proteins in target image is in horizontal alignment state under pixel space coordinate system;Determine the development area of each target protein in target image and the irregular region of interest wrapped the protein band corresponding to multiple target proteins, and for each target protein, the expression abundance of target protein is obtained by analyzing the development area and the irregular region of interest, and the accuracy of the protein expression abundance is higher.

The application provides a programmable hydrogel wound dressing with rotary contractive force and application thereof, and belongs to the technical field of biomedical materials, and comprises: a double-network hydrogel main body composed of a first network and a second network; the first network is a chemically cross-linked methacrylated hyaluronic acid network, and the cross-linked network structure is formed through photo polymerization; the second network is a physically cross-linked polyvinyl alcohol network, and the instantaneous cross-linking points are formed through hydrogen bonds and crystal regions, and the hydrogel is endowed with shape memory performance; the surface is modified with N-hydroxysuccinimide ester functional groups, which can form covalent bonds with the amino groups of tissue proteins on a wound surface; the double-network hydrogel main body is dried and shaped into a preset shape after being pre-stretched or pre-twisted, restores the initial shape after absorbing moisture, and outputs a rotary contractive mechanical force. The application realizes rapid wound healing and scar-free regeneration by dynamically regulating the mechanical force at the wound edge and enhancing tissue adhesion, and further realizes the application in regulating the wound microenvironment to promote scar-free healing.

The invention provides tissue protein using soybean processing byproducts as raw materials and a preparation method of the tissue protein, and belongs to the technical field of food processing. The tissue protein is prepared from the following raw materials in parts by weight: 8 parts of soybean protein isolate, 4 parts of vital gluten, 8 parts of fresh and wet bean dregs and 7 parts of yellow serofluid, the fresh and wet bean dregs are obtained by soaking raw material dry soybeans in water, grinding into thick liquid and filtering, the rest product is soybean milk, and the yellow serofluid is a liquid byproduct collected in the bean curd pressing process. The bean curd is prepared by taking the soybean milk as a raw material. According to the method, after the soybean byproducts and the raw materials for preparing the tissue protein are treated by the single screw extruder, harmlessness is completely realized, and the nutritional value and functional characteristics of the tissue protein are favorably improved.

The invention discloses application of K224 lactic acid modification of HNRNPF as a target spot in preparation of a product for diagnosis and / or treatment of pancreatic cancer, and belongs to the technical field of tumor precision medical products. The embodiment of the invention proves that the lactylation modification level of the K224th site of the HNRNPF protein in pancreatic cancer tissues is obviously higher than that of para-carcinoma tissues, the lactylation modification level of the 224th lysine of the HNRNPF protein is positively related to the progression of pancreatic cancer, and the multiplication capacity of pancreatic cancer cells can be obviously inhibited by constructing the HNRNPF K224R site mutant. On the basis, the reagent for detecting the HNRNPF K224 lactylation level can be used for preparing products for diagnosing and / or treating pancreatic cancer, such as a reagent for detecting or inhibiting the modification and the like. The application provides a novel specific marker and an intervention strategy for early diagnosis and targeted therapy of pancreatic cancer, and has important significance and application value.

The present application relates to a method for producing fermented plant tissue protein with enhanced adhesiveness. According to the present application, the adhesiveness of the plant tissue protein is improved by the mold mycelium formed between the plant tissue protein (TVP) particles and the squeezing process, and in one embodiment, a plant tissue protein having a soft texture similar to that of meat tissue can be produced.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

This application discloses the application of HNRNPF K224 lactation modification as a target in the preparation of products for the diagnosis and / or treatment of pancreatic cancer, belonging to the field of precision oncology product technology. Examples of this application demonstrate that the lactation modification level of HNRNPF protein at site K224 is significantly higher in pancreatic cancer tissue than in adjacent normal tissue. The lactation modification level of lysine 224 in HNRNPF protein is positively correlated with the progression of pancreatic cancer. Constructing the HNRNPF K224R point mutant can significantly inhibit the proliferation of pancreatic cancer cells. Based on this, this application provides reagents for detecting HNRNPF K224 lactation levels, which can be used to prepare products for the diagnosis and / or treatment of pancreatic cancer, such as reagents for detecting or inhibiting this modification. This application provides new specific biomarkers and intervention strategies for the early diagnosis and targeted therapy of pancreatic cancer, and has significant meaning and application value.

The application provides a sealing material for wet tissues, a preparation method and application thereof, and the sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres; the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microspheres have a core-shell structure, the core-shell structure comprises a core and a shell covering the core; the core comprises a temperature-sensitive cross-linked network and an ion-cross-linkable polysaccharide, and the shell is a porous shell body network and is formed by a copolymerization reaction of a light-cross-linkable natural polymer derivative and a co-monomer containing an active chemical group. The application realizes rapid hemostasis on dynamic and bleeding wet tissues through a double drainage mechanism of the hydrophobic oil phase instantaneously draining liquid and the temperature-sensitive core actively pumping, and realizes sealing on the wet tissues through the shell and tissue protein cross-linking to form stable chemical adhesion.

The utility model discloses a zebrafish embryo and juvenile tissue protein and nucleic acid extraction sleeve which comprises an outer sleeve and an inner sleeve, the inner sleeve is installed on the top of the outer sleeve through a neck tube, and the neck tube comprises a short neck tube and a long neck tube. The inner sleeve is sequentially matched with the short neck pipe and the long neck pipe to be installed in the outer sleeve, the whole of the inner sleeve, the short neck pipe and the outer sleeve is used in cooperation with a centrifugal machine, fish culture water / fry culture liquid is removed, and zebrafish embryos / juvenile fishes without water are obtained at the bottom of the inner sleeve; then the whole body of the inner sleeve, the long-neck tube and the outer sleeve is matched with an automatic grinding instrument for use, so that the zebrafish embryos / juvenile fishes in the inner sleeve extend into the nucleic acid / protein extraction solution and are fully ground and extracted; the whole of the inner sleeve, the short-neck tube and the outer sleeve is used in cooperation with a centrifugal machine, the broken zebrafish embryo / juvenile fish tissue in the inner sleeve is converged to the inner bottom of the outer sleeve, a nucleic acid / protein mixed solution is obtained, the outer sleeve is centrifuged, and supernatant is taken, so that the nucleic acid / protein solution can be obtained.

The present application belongs to the technical field of biological medicine, and particularly relates to a composition for improving cognitive function, a preparation method, products and applications thereof. The composition is prepared from soybean meal powder, whey protein peptide powder and bovine collagen peptide powder in a mass ratio of 1:3:2 as a peptide source raw material, in combination with AMY001, Lactobacillus plantarum LP-90 and Clostridium butyricum (3:1:1) composite strains, through Bacillus amyloliquefaciens enzyme production self-digestion, peptide-bacteria anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. The composition can work synergistically through multiple mechanisms such as clearing hippocampal Aβ1-42 protein, increasing BDNF content and inhibiting IL-6 expression, has high memory improvement rate and Aβ1-42 clearance rate, and can significantly repair cognitive impairment. The composition has high stability and bioavailability, the preparation process is controllable, and is suitable for the development of drugs for preventing or assisting in improving cognitive impairment related diseases.

The utility model discloses a vein stripping hemostasis device which comprises a catheter, one end of the catheter is fixedly connected with a guide head, the surface of the catheter is provided with an embedding groove, a wire is arranged in the embedding groove, one side of the wire is fixedly connected with an electrode, the surface of the guide head is provided with a containing groove, and the electrode is fixedly connected with the containing groove. The electrode is located in the containing groove, a connecting base is movably arranged on the surface of the guide pipe, a power line is fixedly connected to the surface of the connecting base, and the wire extends into the connecting base and is electrically connected with the power line. The guide head is arranged to drive the catheter to enter a tunnel generated in a vein stripping operation, the connecting seat is moved to drive the electrode to extend out of the containing groove, and the electrode is arranged to make contact with a bleeding point and transmit energy, so that tissue protein at the bleeding point is solidified, and the aim of stopping bleeding is achieved. Hematoma can be avoided.

The application discloses a biological macromolecular drug for tumor treatment, which has tissue protein adhesion, can continuously improve the oxidative stress environment of a tumor site by prolonging the time at the tumor site, can inhibit tumor growth by tumor microenvironment regulation, can also be used as an auxiliary therapeutic agent to enhance the curative effect of other tumor treatments, and has a good clinical transformation prospect.

This invention discloses a plant-based meat block and its preparation method, belonging to the field of food processing. The plant-based meat block, by weight, is composed of 35-45 parts water, 25-35 parts textured protein, 8-13 parts egg white powder, 5-11 parts vegetable oil, 5-8 parts sugar, 4-7.5 parts TG enzyme, 1.5-2 parts gel-type soy protein isolate obtained from high-oleic soybeans, and seasonings. The preparation method includes steps such as soaking textured protein, frying at 165-175℃ for 6-8 min, braising at 55-65℃ for 30-40 min, mixing, settling at 3-5℃, steaming at 120℃ for 50 min, and sterilization. This invention, through a reasonable ratio of egg white powder and soy protein isolate and the introduction of TG enzyme for cross-linking, reduces the amount of exogenous additives. The resulting product has a firm structure, is not easily broken when cut, has a high similarity in taste to meat, and the processing technology is simple, facilitating industrial production.

The utility model relates to the technical field of textured protein processing, in particular to a textured protein splitting and dewatering device which comprises a fixing frame, a noise reduction assembly is arranged at the lower end of the fixing frame, and the noise reduction assembly comprises a first connecting plate, a damper, a mounting base, a spring and a second connecting plate. By arranging the damper and the spring, a good noise reduction effect can be achieved for the device, when tissue protein is subjected to thread removal and dehydration work, high-speed rotating mechanical motion enables the device to vibrate so as to make noise, and when the device operates, the vibration generated by the mechanical motion can be transmitted to the first connecting plate, so that the device is prevented from being damaged. The first connecting plate absorbs vibration through the damper and the spring, the damper can reduce the vibration amplitude, and the damper is matched with the spring, so that the noise reduction assembly can effectively reduce noise generated during operation of the device, a more comfortable working environment is created for operators, meanwhile, the device can be protected against damage caused by vibration, and the service life of the device is prolonged.

The present invention addresses the problem of providing a textured protein material that can be used as a meat substitute in a raw material for the production of processed foods, or as a meat substitute product as is, and has improved flavor in comparison with conventional meat substitutes.　Provided is a production method for a flavored textured protein material in which a texturized raw material containing a protein raw material is texturized by means of introducing said texturized raw material into an extruder, and subjecting the same to kneading, pressing, and heating, said textured protein material production method being characterized in that a powdered protein raw material flavored by being brought into contact with a flavoring gas in advance is added so as to be least 3 wt% of the protein raw material.