347 results about "Proteome" patented technology

The present disclosure is directed to methods for measuring proteomes of single cells. In one aspect, methods comprise disposing cells on a surface, synthesizing hydrogel chambers around each of the cells, lysing the cells to release intracellular proteins for adsorption onto the surface bounded by the hydrogel chambers, depolymerizing the hydrogel chambers, and quantifying the adsorbed proteins with detection antibodies to determine the single cell proteomes.

This invention discloses a milk protein containing composition comprising: at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence coded by nucleotide sequence which is at least 80% similar to sequences selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention provides a rapid quantitative analysis method for an outer vesicle proteome, and belongs to the technical field of quantitative analysis of the outer vesicle proteome. According to the rapid quantitative analysis method for the outer vesicle proteome, firstly, a high-quality outer vesicle sample is obtained through low-speed centrifugation, ultra-speed centrifugation and membrane filtration; and obtaining proteome data by utilizing two-dimensional electrophoresis and mass spectrometry. Then, a mathematical model equation set is constructed for preliminary analysis, a protein interaction network is optimized through an embedded game optimization index model, and feature extraction and quantitative analysis are conducted through a convolution parameter adaptive function and a residual network deep learning model; finally, a quantitative analysis result containing the protein expression level and the function importance is generated in combination with the multi-dimensional protein expression contribution index, and the technical problem that in the prior art, in the rapid quantitative analysis process of the outer vesicle proteome, the protein component complexity is high, and consequently the quantitative precision is low is solved.

The invention relates to the field of agricultural products, and particularly discloses a polypeptide for improving plant disease resistance, and the polypeptide is LAND2 small peptide and homologous peptide thereof. The LAND2 small peptide disclosed by the invention is screened and found from an arabidopsis thaliana proteome, and homologous peptides of the LAND2 small peptide are respectively derived from chilies and cucumbers. Tests prove that when the LAND2 small peptide is externally applied to arabidopsis thaliana, the local and systematic resistance of plants to botrytis cinerea can be enhanced, and the LAND2 small peptide has broad-spectrum immunocompetence in chilies and cucumbers. The polypeptide is practically applied to agricultural production, can enhance the disease resistance of plants and improve the yield and quality of the plants, and has practical popularization and application values.

The invention provides a method for screening high-activity endogenous antibacterial peptides, which comprises the following steps: (1) collecting antibacterial peptide sequences in an antibacterial peptide database, and constructing an antibacterial peptide prediction model; (2) acquiring pig source proteome data; (3) screening out sequences with the length of 8-50 residues from pig-source proteome data to construct a candidate data set; (4) screening out non-redundant sequences from the candidate data set to construct a prediction data set; (5) predicting endogenous antibacterial peptides in the prediction data set by using the antibacterial peptide prediction model to obtain candidate antibacterial peptides; and (6) carrying out bioinformatics analysis on the candidate antibacterial peptide, comparing the candidate antibacterial peptide with an antibacterial peptide database, and screening out the antibacterial peptide with the similarity of less than 100% and secretory protein characteristics or exosome protein characteristics to obtain the high-activity endogenous antibacterial peptide. The method provided by the invention can be used for efficiently and quickly screening the high-activity endogenous antibacterial peptide, provides a new way for developing novel antibacterial peptide, and has a wide application prospect.

Provided herein are novel stabiligases for use in cell membrane proteome analysis. The subject stabiligases are capable of attaching to glycans found on the surface of cell membranes to form glycan-tethered (GT) stabiligases. Such glycan-tethered stabiligases are capable of robustly and selectively attaching label probes to cell surface proteins of intact cells. The subject novel stabiligases described herein advantageously allow for the identification and profiling of cell membrane proteins that have undergone an extracellular N-terminal proteolytic event.

The invention discloses a biological age assessment method, which comprises the following steps: acquiring a key protein index of a to-be-assessed person, and inputting an improved biological age assessment model I to obtain the biological age of the to-be-assessed person; the improved biological age assessment model I is constructed and obtained based on an elastic network regression algorithm by taking proteome data and body composition age of an individual as training data. The invention also discloses a construction method of the model, namely a two-step method. According to the method, the user is allowed to calculate the biological age according to the proteome data of the user, personalized monitoring of the muscle aging state is achieved, and the method has great guiding significance in the aspects of identifying individuals with premature aging of muscle functions and formulating personalized precise anti-aging schemes.

The present invention relates to nucleic acids coding for a protein said protein comprising a truncated glial fibrillary acidic protein (GFAP), proteins comprising a truncated GFAP, expression constructs comprising a nucleic acid coding for a protein which comprises a truncated GFAP, vectors comprising the nucleic acids or expression constructs disclosed herein and eukaryotic cells comprising a nucleic acid, proteins, expression construct or vector disclosed herein. The present invention furthermore relates to compositions comprising the herein disclosed proteins and kits comprising the herein disclosed proteins and / or compositions. Furthermore, the present invention relates to the use of the herein disclosed proteins, compositions and / or kits for calibrating a measurement system for the in vitro quantification of GFAP or a variant thereof in a sample.

According to the screening method for the potential protein biomarkers and the drug targets of the gastric cancer, Mendel randomization analysis in a proteome range is adopted, the genetic causal relationship between circulating plasma protein and the risk of the gastric cancer is evaluated, and finally the remarkably related protein is identified. According to the screening method of the potential protein biomarker and the drug target of the gastric cancer, provided by the invention, the potential association between circulating plasma protein and the gastric cancer is systematically revealed by integrating Mendel randomization, single-cell RNA sequencing analysis, space transcriptome analysis, virtual drug screening, molecular docking, molecular dynamics simulation and other methods.

This disclosure pertains to compositions comprising an anti-FcRn antibody, M281. The compositions include the full, intact antibody and size variants thereof that not include two antibody heavy chains and to antibody light chains. Thus, a M281 pharmaceutical composition can include: an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO:2 and a light chain comprising the amino acid sequence of SEQ ID NO:1, wherein the composition comprises a major protein component having a molecular weight of 140,000-145,000 Da and a minor protein component of molecular weight 118,000-120,000 Da.

The presently described and claimed disclosure relates to capillary electrophoresis methods for quantifying an intact AAV genome and protein components in an AAV using the same capillary electrophoresis system. The claimed and described approach offers an automated analysis of AAV samples and provides information to determine the AAV empty / full ratio.

The present disclosure provides methods of identifying a disease-specific immunogenic peptide through a series of selection steps. Immunogenic epitopes identified by methods of the present disclosure are applicable for use in peptide-based immunotherapy, preferably cancer therapy. Furthermore, the methods of the present disclosure may be performed in a high-throughput manner and serve as a means of personalized vaccine development and therapy. Also provided are compositions of immunogenic peptides as well as methods of treatment comprising said compositions.

The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.

The invention relates to the technical field of optics, and discloses a visualization method of a biological tissue three-dimensional full-information atlas, which comprises the following steps of: acquiring image data, electron microscope data, mechanical data, protein distribution, omics information and histological staining images of biological tissues by using various biological visualization technologies, and correspondingly processing the acquired data to obtain a three-dimensional full-information atlas of the biological tissues; finally, organization information fusion and digital display are achieved. According to the method, a cross-scale feature correlation algorithm framework is innovatively established, and multi-source heterogeneous data fusion is performed on medical image topological data (CT / MRI), ultrastructure scanning data (SEM / TEM), molecular positioning information (immunofluorescence, immunohistochemistry and enzyme immunoassay), omics maps (space transcriptome / proteome) and high-resolution tissue staining data. The data island dilemma caused by traditional single-mode analysis is broken through, and full-scale visualization from nanoscale molecular distribution to centimeter-level organ tissue structure-function relationship is realized for the first time.

A direct-read precision de novo sequencing method for amino acid sequences of proteins and proteome, the method involving determining the type of product ions on the basis of lysine (K) and arginine (R) dual mass-defect amino acid encoding (NeuCode) labeling, and performing paired mirror digestion to achieve the direct-read precision de novo sequencing of animo acid sequences of proteins with full product-ion coverage. A protein is labeled with K / R NeuCode amino acids, mirror peptide fragments are obtained by means of paired mirror digestion, and the mirror peptide fragments are subjected to tandem mass spectrometry analysis to obtain a mirror spectrum with full product-ion coverage and product ion type identification. Consequently, the amino acid sequence information in a secondary spectrum can be directly read on the basis of the mass difference of two adjacent product ions of the same type, thereby realizing direct reading and precision sequencing of the amino acid sequence of the protein.

Provided herein are compositions, systems, and methods for delivering an effector protein into a cell. The present disclosure, in some aspects, provide novel proteins delivering an effector protein into a cell. The novel proteins are supernegatively charged proteins derived from highly anionic proteins identified from the proteome (e.g., human proteome). The novel protein tags can be associated (e.g., covalently or nocovalently) with the protein to be delivered to facilitate delivery of the effector protein into a cell.

The invention relates to the field of gene engineering, in particular to a proximity marker enzyme mutant and application thereof. The proximity marker enzyme mutant takes ProtA-Turbo as a starting sequence, and one or more sites in K71, K240, K335, K345 and K351 are mutated into non-polar hydrophobic amino acid, polar neutral amino acid, acidic amino acid, basic amino acid or non-natural amino acid. The proximity marker enzyme mutant overcomes the influence of self biotinylation on the proximity marker activity, can continuously and stably mark the proximity proteome of the target protein on the immobilized cell, and obviously improves the sensitivity of identifying the proximity interacting protein of the target protein.

The present invention relates to the preparation and application of a micro-cell in-situ microreactor. By subjecting the inner wall of a capillary to silanization treatment and modifying epoxy groups on its surface through free radical polymerization reaction, an open-tubular capillary column is prepared. Then, polyethyleneimine and iodoacetic acid-N-succinimidyl ester are successively modified by covalent bonding to prepare a solid-phase alkylated capillary microreactor. The advantages of the present invention are that proteomic sample processing is carried out in-situ, which is conducive to reducing sample loss caused by multiple transfers during traditional sample processing. Therefore, this solid-phase alkylated open-tubular column is suitable for proteomic pretreatment of trace samples. In addition, this method is tolerant to various surfactants and strong lysis reagents and has good sample compatibility.

The present application relates to a kind of anaphalis contorta exosome extraction method and its application.The exosome is obtained by ultra-high speed centrifugation method from fresh anaphalis contorta, particle size is 160-200 nm, Zeta potential is-21.63±1.34 mV, with double membrane structure, mainly containing protein (such as resveratrol O-methyltransferase etc.) and phospholipid, polysaccharide content is only 12.5% of anaphalis contorta fresh medicine, and does not contain traditional active ingredient anaphalis contorta glycoside.Experiments show that anaphalis contorta exosome can be targeted to enrich in colorectal tissue, significantly improve AOM / DSS induced colorectal cancer model mouse tumor microenvironment, reduce tumor number, regulate T cell and macrophage ratio, and relieve pathological damage, better than anaphalis contorta fresh medicine.The mechanism is related to protein component, not polysaccharide or small molecule component.The anaphalis contorta exosome provided in the present application has simple preparation process, high safety, and has significant anti-colorectal cancer application potential.

The invention relates to the field of aquatic food traceability, in particular to a system for identifying wild and culture sources of bighead carps and application of the system. The system comprises: a sample processing module, wherein the sample processing module is at least used for processing a bighead carp sample to obtain original data of a polypeptide as shown in any one of SEQ ID NO.1-7, and obtaining proteome component response of a to-be-detected sample; the data processing module is at least used for importing the proteome component response of the sample to be detected into an OPLS-DA classification discriminant analysis model to obtain a score plot for discriminant analysis of subsequent sample data; and the result output module is at least used for outputting a result. The system can be effectively used for identifying wild and culture sources of bighead carps, is high in stability and specificity, does not need to use a large instrument, is low in detection cost, and has a wide application prospect in the aspect of aquatic product traceability.

Systems, media, compositions, methods, and kits disclosed herein relate to a panel of protein biomarkers for the early detection of colon cell proliferative disorders, including colorectal cancer. The presence or levels of the proteins in a biological sample for the protein panels described herein may be used for classifier generation, and as inputs in machine learning models useful to classify subjects in a population for the detection of colon cell proliferative disorders. 4

Methods of determining the stoichiometry of a viral capsid and / or determining the heterogeneity of protein components in a viral capsid are described.

The invention discloses an auxiliary protein composition, iron oxide nanoparticles, a preparation method and application, the composition comprises an SC protein with an amino acid sequence as shown in SEQ ID NO: 1 and an ST protein with an amino acid sequence as shown in SEQ ID NO: 2, and the composition can be used for modifying the iron oxide nanoparticles. The auxiliary protein composition can effectively promote endocytosis of the iron oxide nanoparticles, and assists the iron oxide nanoparticles to realize lysosome escape; moreover, the iron oxide nanoparticles modified by the auxiliary protein composition are good in biocompatibility and long in retention and storage time in cells, can be used for long-time labeling of the cells, and have wide clinical transformation and application prospects as a cell tracer agent.

Methods of labeling, processing and analyzing protein samples. Surface-accessible residues in a protein sample (such as a proteome) can be labeled using a carbodiimide coupling agent an amine comprising a click chemistry handle, such as glycine propargyl amide. The click chemistry handle can then be derivatized using high-yield "click" chemistry to form a stable linkage with an enrichable label. The proteome can then be digested, peptides that have been modified can be enriched, and the enriched peptides can be analyzed, for example, by mass spectrometry. The analyzed peptides can constitute a highly enriched subset of the original peptides while still representing many sites of modification on many proteins (~95%) originally present in the proteome. The methods can be used to identify solvent-accessible regions of proteins, conformational changes, and drug targets across a whole proteome.

The present invention relates to nucleic acids coding for a protein said protein comprising a glial fibrillary acidic protein (GFAP) and a eukaryotic secretion tag, proteins comprising a GFAP and a eukaryotic secretion tag, expression constructs comprising a nucleic acid coding for a protein which comprises a GFAP and a eukaryotic secretion tag, vectors comprising the nucleic acids or expression constructs disclosed herein and eukaryotic cells comprising a nucleic acid, expression construct or vector disclosed herein. The present invention furthermore relates to methods for the production of the herein disclosed proteins. The present invention also relates to compositions comprising the herein disclosed proteins and kits comprising the herein disclosed proteins and / or compositions. Furthermore, the present invention relates to the use of the herein disclosed proteins, compositions and / or kits for calibrating a measurement system for the in vitro quantification of GFAP or a variant thereof in a sample.

Provided herein are methods for identifying antimicrobial peptides deriving from extinct proteomes using a multitask deep learning approach. Also disclosed are antimicrobial peptides, and methods of treating an antimicrobial infection comprising contacting the infection with the present antimicrobial peptides. Also disclosed are methods of treating a microbial infection comprising administering to a subject in need thereof a pharmaceutically effective amount of an antimicrobial peptide according to the present disclosure.

The invention relates to the technical field of organelle separation, and provides a method for rapid purification of peroxisome and oxidation regulation analysis of ultra-long chain fatty acid. The method comprises the following steps: introducing a nucleic acid sequence for coding a fusion protein into a target cell to obtain a cell material, the fusion protein comprising a truncated sequence derived from a peroxisome membrane protein and a tag sequence for affinity purification; taking the cell material as a sample, and separating the peroxisome with the fusion protein from the cell by utilizing an affinity ligand on the basis of a high affinity binding principle. Therefore, according to the method disclosed by the invention, the peroxisome can be rapidly and efficiently enriched, and the whole purification process can be completed within 30 minutes, so that the experimental period is greatly shortened; core protein components of target organelles can be efficiently reserved, and the metabolic activity of peroxisome can be maintained through a complete membrane structure and an internal environment; meanwhile, the same purified sample can synchronously support proteome and metabolome analysis, and direct association of'protein-metabolism 'data is realized.

Described herein is a method of preventing or treating Human Papilloma Virus (HPV) infection in a subject comprising selecting optimal HPV cytotoxic T lymphocyte (CTL) epitopes from an HPV proteome for use in a T cell immunogen composition and methods of administering the composition of subjects infected or at risk of infection with HPV.