45 results about "Peritoneal fluid" patented technology

A peritoneal-based (“bloodless”) artificial kidney processes peritoneal fluid without need for additional fluids (“waterless”). Fluid is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s) (“dialysate-pheresis”). It is then reconstituted with additives and returned into the peritoneal cavity, thereby reducing protein-loss and providing oncotic-pressure for ultrafiltration. The protein-free stream is used to produce free water, and an alkaline or acid fluid for optimization of the composition of the regenerated stream. The unused protein-free stream can be used to “reverse flush” the separator to maintain its patency and the excess discarded for fluid-balance regulation. Compared to prior art, immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

Embodiments of the present invention relate generally to certain types of reciprocating positive-displacement pumps (which may be referred to hereinafter as “pods,”“pump pods,” or “pod pumps”) used to pump fluids, such as a biological fluid (e.g., blood or peritoneal fluid), a therapeutic fluid (e.g., a medication solution), or a surfactant fluid. The pumps may be configured specifically to impart low shear forces and low turbulence on the fluid as the fluid is pumped from an inlet to an outlet. Such pumps may be particularly useful in pumping fluids that may be damaged by such shear forces (e.g., blood, and particularly heated blood, which is prone to hemolysis) or turbulence (e.g., surfactants or other fluids that may foam or otherwise be damaged or become unstable in the presence of turbulence).

A peritoneal-based (“bloodless”) artificial kidney processes peritoneal fluid without need for additional fluids (“waterless”). Fluid is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s) (“dialysate-pheresis”). It is then reconstituted with additives and returned into the peritoneal cavity, thereby reducing protein-loss and providing oncotic-pressure for ultrafiltration. The protein-free stream is used to produce free water, and an alkaline or acid fluid for optimization of the composition of the regenerated stream. The unused protein-free stream can be used to “reverse flush” the separator to maintain its patency and the excess discarded for fluid-balance regulation. Compared to prior art, immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

A device may be implanted subcutaneously with an attached catheter inserted within, e.g., the peritoneal cavity of a subject. The catheter and / or device may also be inserted into another space, e.g., subcutaneous, vascular, peritoneal, cerebrospinal, pleural spaces, etc. The peritoneal fluid which normally collects and / or flows through the peritoneal cavity may be detected by the catheter and analyzed via the device to detect the concentration of glucose within the fluid.

An automated peritoneal dialysis system provides various features. The features includes prescription-driven dialysis fluid preparation, an integrated disposable fluid circuit, and sensor capabilities that allow accurate filing and draining control with high safety margins. The features include a peritoneal fluid circuit with a pressure sensor at either end and methods and devices for using the pressure signals. Other features and embodiments are disclosed.

Methods and devices for the diagnosis and treatment of diabetes are disclosed in which an analyte concentration within a peritoneal fluid of a human subject may be determined by implanting an analyte sensor apparatus in the subject where the apparatus may comprise a housing and a flexible sensing catheter which has a lumen with a plurality of apertures and an exterior surface with an analyte sensor affixed thereto. The catheter may comprise a proximal end attached to the housing and the remaining end may be positioned freely within the peritoneal space to contact peritoneal fluid where an analyte concentration in the peritoneal fluid may be sensed. The housing may be anchored at a subcutaneous site proximate the peritoneal space. The sensed analyte concentration may then be transduced into a transmittable electrical signal.

A peritoneal-based artificial kidney processes peritoneal fluid without need for additional fluids. Spent dialysate is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s). Alternatively, the spent dialysate is first processed in a sorbent assembly and then separated into the protein-rich and protein-free streams. Immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

A peritoneal-based artificial kidney processes peritoneal fluid without need for additional fluids. Spent dialysate is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s). Alternatively, the spent dialysate is first processed in a sorbent assembly and then separated into the protein-rich and protein-free streams. Immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or ''unmask'' epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

Method and apparatus for ultrafiltration of a patient being overhydrated due to congestive heart failure, comprising a cassette having four inlets / outlets. A patient tube is connected to a patient connector, intended to be connected to a patient line for access to a peritoneal cavity of the patient. The patient tube comprises a flow pump for addition and removal of a peritoneal fluid between the cassette and the peritoneal cavity. The fluid is introduced into an intermittent bag controlled by an intermittent valve and then returned the same way back to the peritoneal cavity. Glucose is metered into the fluid entering the peritoneal cavity by means of a glucose pump. Glucose is replenished continuously or intermittently for keeping a concentration of the osmotic agent substantially constant in the peritoneal cavity. After treatment, the peritoneal fluid is drained to a drain bag, wherein the drain tube comprises a drain valve and an albumin filter.

The invention relates to a medical facility, in particular to a household automated peritoneal dialysis device. In the raw water treatment device of the facility, the water inlet of a multilayer filtration device is directly connected with a water source, the water outlet of a reverse osmosis device is connected with a peritoneal dialysis fluid mixer; the main body of a peritoneal dialysis fluid preparing device is a sealed metal box body, the metal box body is provided with interfaces which are connected with a plunger pump, a penetrant container, a peritoneal dialysis agent container, the peritoneal dialysis fluid mixer, an ultrafiltration device, a waste liquid container and an abdominal catheter through plunger valves; and the signal input interface of the ARM single chip microcomputer of a peritoneal dialysis automatic control system is connected with a treatment procedure module, a reparative treatment procedure module and a safety monitoring module respectively, and the signal output interface is connected with a power driver which is driven according to the commands of the ARM single chip microcomputer to control the plunger pump, the plunger valves, an electric heater, a solenoid valve and a sound and light alarm device. The invention integrates raw water treatment, peritoneal fluid preparation and automated dialysis so as to reduce medical cost and ensure safety and is suitable to be used in the basic units.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

The present invention relates to a non-invasive diagnosis method of endometriosis by detecting biochemical marker in serum or peritoneal fluid, in particular alpha 1-antitrypsin, fragments of alpha 1-antitrypsin, or a combination of both. The diagnosis of endometriosis is performed with observing in serum specimens of a patient the concentration and change of the biochemical marker, in particular molecules related to alpha 1-antitrypsin, and comparing with a predetermined baseline level of the biochemical marker contained in serum. Statistical analysis can be performed to evaluate the baseline level indicating the occurrence of endometriosis. Therefore, the present invention can provide an auxiliary guideline for the diagnosis of endometriosis. The present invention also relates to usage of the biochemical marker.

The invention discloses compound traditional Chinese medicine for treating endometriosis. The compound traditional Chinese medicine comprises, by weight, 8-12 parts of Pollen Typhae, 8-12 parts of trogopterus dung, 1-3 parts of dragon's blood, 12-20 parts of astragali radix, 8-12 parts of dipsacus root, 8-12 parts of roasted myrrh, 8-12 parts of corydalis yanhusuo, and 8-12 parts of szechwan chinaberry fruit. The invention further discloses a preparation method and application of the compound traditional Chinese medicine. The compound traditional Chinese medicine brings the advantages of multi-targeted treating of traditional Chinese medicine into full play. Lab observation of development of ectopic endometrium in a drug inhibited rat endometriosis model shows that the compound traditional Chinese medicine can inhibit development of endometriosis in terms of inhibiting cell proliferation, angiogenesis and invasion in ectopic endometrium tissues, lowering inflammatory cell factor level in peritoneal fluid and the like.

A diagnostic test and method is provided comprising mixing blood or another biological fluid sample with a test compound and spotting the blood on filter paper for subsequent analysis of the effect of the test compound on the blood. The biological fluid can be a cerebrospinal fluid, a peritoneal fluid, a cyst fluid, an amniotic fluid, a lavage fluid, a saliva, a cell extract or a tissue extract. The compound is chosen among an amino acid, a peptide, a protein, a carbohydrate, an oligosaccharide, a polysaccharide, a glycoprotein, a lipid, a lipoprotein, a glycosaminoglycan, a hormone, a steroid, a vitamin, a low molecular weight synthetic or natural compound which influences the blood to cause an alteration of its composition, e.g., a toxin, allergen, autoantigen, bacterial protein or polysaccharide, viral protein, fungal protein or polysaccharide, parasitic protein or polysaccharide, bacterial lipopolysaccharide or any other compound relevant to diseases.

The invention belongs to the field of natural medicinal chemistry, in particular to separation and extraction of natural anti-inflammatory drug molecules and an application thereof. The method comprises the following steps of extracting by acidic ethanol, decompressing and concentrating, adding water for dissolving, loading a sample in XAD-7 macroporous resin, eluting, collecting and concentrating, freeze-drying to extract the mangosteen pericarp anthocyanin. Experimental results show that mouse peritoneal fluid neutrophil exudation can be effectively inhibited after the mangosteen pericarp anthocyanin is injected into caudal vein of the mouse, the inhibition ratios are respectively 34%, 77% and 94% while the dosages are respectively 0.01, 0.1 and 1mg / ml. A laminar experiment using an in-vitro simulated capillary proves that the adhesion inhibition ratios of the mangosteen pericarp anthocyanin on neutrophil are respectively 45%, 71% and 89% while the dosages are respectively 0.01, 0.1 and 1mg / ml. Therefore, the anti-inflammatory activity of the mangosteen pericarp anthocyanin is proved, and the mangosteen pericarp anthocyanin as a novel anti-inflammatory drug has good application prospect.

The invention belongs to the field of natural medicinal chemistry and particularly relates to a separation and purification process of natural polysaccharide type anti-inflammatory drug molecules and application thereof. The separation and purification process utilizes water extraction and alcohol sedimentation, protein removal, acid hydrolysis, freeze drying, desalination, affinity chromatography and other methods to purify a polysaccharide fragment having a P-selectin antagonism function from bupleurun chinense. Flow cytometry and protein interaction analysis experiment results show that the bupleurun chinense polysaccharide fragment can effectively prevent the combination between P-selectin and HL-60 cells and the antagonism to the mutual effect of the P-selectin and PSGL-1. The experimental results of a parallel-plate flow chamber and an acute abdominal inflammation mouse model show that the bupleurun chinense polysaccharide fragment has the remarkable inhibitory effect on the HL-60 cells mediated by the P-selectin in a shear stress state and can effectively inhibit leukocytic exudation from the peritoneal fluid of a mouse. The bupleurun chinense polysaccharide fragment prepared by means of the method has good anti-inflammatory activity and has a broad application prospect by serving as a novel anti-inflammatory drug.

An apparatus for ultrafiltration of a patient being overhydrated due to congestive heart failure. The apparatus comprises a dilution syringe for removal of a portion of peritoneal fluid from the peritoneal cavity. A glucose bag comprises glucose concentrate at a concentration of 30 %. A small amount of glucose concentrate is mixed with the dilution fluid in the dilution syringe in order to dilute the glucose concentrate to below 3 % concentration. Then, the mixture is filled into the peritoneal cavity from the dilution syringe in order to replenish the glucose in the peritoneal cavity for maintaining a substantially constant glucose concentration in the peritoneal cavity. In addition, peritoneal fluid is intermittently removed from the peritoneal cavity for counteracting increased intraperitoneal fluid volume and increased intraperitoneal pressure due to ultrafiltration. A UF bag is arranged for receiving such surplus peritoneal fluid. A glucose syringe may be arranged for metering the glucose concentrate.

The invention provides a device capable of injecting a preservative solution and sucking pleural and peritoneal fluid. The device comprises an air pump, a fluid collecting tank, an infusion tank and a suction needle head, wherein the air pump comprises an air inlet for feeding air and an air outlet for exhausting air; a negative-pressure hole connected with the air inlet of the air pump and a fluid suction hole connected with the suction needle head are formed in the liquid collecting tank; a positive-pressure hole connected with the air outlet of the air pump and an infusion tube connected with the suction needle head are formed in the infusion tank; the preservative solution is contained in the infusion tank; the infusion tube is positioned under the liquid level of the preservative solution; an air inlet valve which is directly communicated with the outside is further arranged at the air inlet of the air pump; an exhaust valve which is directly communicated with the outside is further arranged at the air outlet of the air pump. Pleural and peritoneal fluid is sucked and the preservative solution is injected according to an air pressure principle, so that the liquid does not need to contact the air pump and other power equipment, and the service life of the device is prolonged greatly.

The invention relates to a medical facility, in particular to a household automated peritoneal dialysis device. In the raw water treatment device of the facility, the water inlet of a multilayer filtration device is directly connected with a water source, the water outlet of a reverse osmosis device is connected with a peritoneal dialysis fluid mixer; the main body of a peritoneal dialysis fluid preparing device is a sealed metal box body, the metal box body is provided with interfaces which are connected with a plunger pump, a penetrant container, a peritoneal dialysis agent container, the peritoneal dialysis fluid mixer, an ultrafiltration device, a waste liquid container and an abdominal catheter through plunger valves; and the signal input interface of the ARM single chip microcomputer of a peritoneal dialysis automatic control system is connected with a treatment procedure module, a reparative treatment procedure module and a safety monitoring module respectively, and the signal output interface is connected with a power driver which is driven according to the commands of the ARM single chip microcomputer to control the plunger pump, the plunger valves, an electric heater, a solenoid valve and a sound and light alarm device. The invention integrates raw water treatment, peritoneal fluid preparation and automated dialysis so as to reduce medical cost and ensure safety and is suitable to be used in the basic units.

The invention discloses a pneumoperitoneum no-injury aspirator head end device for a laparoscope. The head end device comprises a suction tube, a control valve, a dipping sponge ball and a sponge ball controller. The suction tube is of a middle bracket structure with the back end communicated with the inner cavity of the control valve; the headmost end of the suction tube and the dipping sponge ball are connected, and the headmost end of the suction tube and the dipping sponge ball are screwed by screw threads and are in sealed connection. The dipping sponge ball comprises outer side conical brackets, a connecting webbing membrane between the conical brackets, elastic sponges in the conical brackets and an arc-shaped top constituted by a front-end unidirectional water-permeable composite fiber membrane, so that the unidirectional circulation of sucked peritoneal fluid is guaranteed, the backflow of dipped liquid is prevented and the possibility that a pneumoperitoneum is injured is reduced. The sponge ball controller, which sleeves outside the dipping sponge ball, can be used for controlling the opening / closing of the dipping sponge ball and facilitating the placement or the withdrawal of the pneumoperitoneum no-injury aspirator head end device for the laparoscope from an abdominal puncture hole. The device disclosed by the invention is relatively suitable for gas laparoscopic operations.

Methods and devices for the diagnosis and treatment of diabetes are disclosed in which an analyte concentration within a peritoneal fluid of a human subject may be determined by implanting an analyte sensor apparatus in the subject where the apparatus may comprise a housing and a flexible sensing catheter which has a lumen with a plurality of apertures and an exterior surface with an analyte sensor affixed thereto. The catheter may comprise a proximal end attached to the housing and the remaining end may be positioned freely within the peritoneal space to contact peritoneal fluid where an analyte concentration in the peritoneal fluid may be sensed. The housing may be anchored at a subcutaneous site proximate the peritoneal space. The sensed analyte concentration may then be transduced into a transmittable electrical signal.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an anti-inflammatory active pharmaceutical composition based on radix bupleuri components and a preparation method of the anti-inflammatory active pharmaceutical composition. The components of the composition are from the radix bupleuri, and particularly, the composition consists of the following components in percentages by mass: 10-50% of a radix bupleuri oligosaccharide fragment and 50-90% of radix bupleuri total saponins. According to pharmacological and activity experimental results, the composition can effectively inhibit exudation of white blood cells in the peritoneal fluid of an acute peritonitis mouse model, and shows good anti-inflammatory activity; and the acting mechanism of the composition is closely related to antagonizing leukocyte adhesion mediated by vascular endothelial P-selectin. The results show that the radix bupleuri composition can be used for preparing novel effective medicines for treating acute inflammation, and has wide application prospect.

The invention relates to a multifunctional abdominal retractor. The tail of a retractor body is provided with a joint, a switch and a battery. Two sides of the retractor body are provided with connecting pipes which are fixed to micro suction heads. The bent portion of the retractor body is provided with an LED cold light lamp. By mounting the connecting pipes and the micro suction heads on the retractor body, the joint is connected with an external vacuum aspirator, and the retractor body absorbs flood and peritoneal fluid directly; when power is cut off, the switch is switched on, the LED cold light lamp lights, the purposes of directly absorbing blood and peritoneal fluid and providing light are realized, and defects of other instruments occupying operating interfaces and increasing infections can be avoided.

The invention provides a device capable of injecting a preservative solution and sucking pleural and peritoneal fluid. The device comprises an air pump, a fluid collecting tank, an infusion tank and a suction needle head, wherein the air pump comprises an air inlet for feeding air and an air outlet for exhausting air; a negative-pressure hole connected with the air inlet of the air pump and a fluid suction hole connected with the suction needle head are formed in the liquid collecting tank; a positive-pressure hole connected with the air outlet of the air pump and an infusion tube connected with the suction needle head are formed in the infusion tank; the preservative solution is contained in the infusion tank; the infusion tube is positioned under the liquid level of the preservative solution; an air inlet valve which is directly communicated with the outside is further arranged at the air inlet of the air pump; an exhaust valve which is directly communicated with the outside is further arranged at the air outlet of the air pump. Pleural and peritoneal fluid is sucked and the preservative solution is injected according to an air pressure principle, so that the liquid does not need to contact the air pump and other power equipment, and the service life of the device is prolonged greatly.

The amount of lipid associated sialoprotein (LSP) in body fluids such as cerebrospinal fluid, peritoneal fluid, pleural fluid, bronchial washings, saliva and sputum samples, may be determined by a method which may be automated, involving the following steps to be performed on the sample: adding a mixture of a chlorinated lower alkyl alcohol; centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a protein precipitating agent; mixing the resulting admixture; recovering the resulting precipitate; washing the precipitate with saline solution; centrifuging to recover the precipitate; dissolving the precipitate in water; mixing; adding to the resulting mixture an hydrolysis agent; heating; and determining the amount of lipid associated sialoprotein present by determining the optical density of the sample.

Method and apparatus for ultrafiltration of a patient being overhydrated due to congestive heart failure, comprising a cassette having four inlets / outlets. A patient tube is connected to a patient connector, intended to be connected to a patient line for access to a peritoneal cavity of the patient. The patient tube comprises a flow pump for addition and removal of a peritoneal fluid between the cassette and the peritoneal cavity. The fluid is introduced into an intermittent bag controlled by an intermittent valve and then returned the same way back to the peritoneal cavity. Glucose is metered into the fluid entering the peritoneal cavity by means of a glucose pump. Glucose is replenished continuously or intermittently for keeping a concentration of the osmotic agent substantially constant in the peritoneal cavity. After treatment, the peritoneal fluid is drained to a drain bag, wherein the drain tube comprises a drain valve and an albumin filter.