116 results about "Natural compound" patented technology

The invention is applicable to the technical field of biological medicines, provides application of a natural compound of a targeted colon cancer drug resistance related protein, and particularly relates to application in preparation of drugs for preventing or treating colon cancer. According to the invention, a natural small molecule compound neohesperidin dihydrochalcone targeting the colon cancer drug resistance related protein TAGLN is screened out, and a new scheme is provided for reversing colon cancer drug resistance, so that the situation that no drug is available to the target spot is solved. Secondly, the invention also provides a scheme for combined use of neohesperidin dihydrochalcone and oxaliplatin, the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, and a new choice is provided for clinical treatment of colon cancer.

The invention relates to the technical field of herbicides, in particular to an allelochemical-based organic herbicide and application thereof. The herbicide is prepared from the following active ingredients: L-menthone, pulegone and beta-caryophyllene. The mass ratio of the L-menthone to the pulegone to the beta-caryophyllene is (1 to 4) to (1 to 4) to 1. The three substances are compounded to generate a synergistic effect of '1 + 1 + 1 gt and' 3 ', the herbicidal activity is far better than that of a single agent, and the use concentration of each component is remarkably reduced. All the components are plant source natural compounds, the degradation path is clear, rapid metabolism can be achieved through microbial decomposition in soil and water, and no complex residual product exists.

A method for treating a gastrointestinal condition includes preparing an aqueous extract of bioactive compound by adding dried green tea material to hot water and filtering the aqueous extract; adding a solution of an iron-containing compound to the filtered aqueous extract to form a complex or chelate of the bioactive compounds with iron; and drying the solution at a temperature between 80 to 150 degrees Fahrenheit to obtain a fine granular or powdered state.

This invention belongs to the field of biomedical technology, specifically relating to a compound extracted from *Acer buergerianum* (a type of shrub), its extraction method, and its application in the preparation of drugs for the prevention or treatment of non-alcoholic fatty liver disease. This invention is the first to obtain a natural compound with a novel structure from *Acer buergerianum*, and provides a method for its preparation. Activity studies were conducted, and experimental data show that the compound exhibits low cytotoxicity and no significant cytotoxicity to normal cells. It can inhibit the expression of fatty acid synthases ACC, ACLY, and FAS, thereby inhibiting de novo fatty acid synthesis in cells, suppressing lipid droplet accumulation, and reducing cellular lipid levels. This compound has broad application prospects in the preparation of drugs for the prevention or treatment of non-alcoholic fatty liver disease.

The application belongs to the technical field of biological medicine, and provides application of a natural compound targeting colon cancer drug resistance related protein, in particular, application in preparation of a drug for preventing or treating colon cancer; the application screens out a natural small-molecule compound hesperetin dihydrochalcone targeting colon cancer drug resistance related protein TAGLN, and provides a new scheme for reversing colon cancer drug resistance, so as to solve the situation that no drug is available for the target point. In addition, the application further provides a scheme in which hesperetin dihydrochalcone is combined with oxaliplatin, and the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, thereby providing a new choice for clinical treatment of colon cancer.

The application discloses a preparation method of egg yolk phospholipid polypeptide which is helpful for bone growth and improvement of brain health, and comprises the following steps: (1) raw material pretreatment to obtain a reaction substrate solution; (2) primary enzymolysis to prepare a primary enzymolysis solution; (3) secondary enzymolysis to prepare a secondary enzymolysis solution; (4) inactivation, the temperature of the secondary enzymolysis solution prepared in the step (3) is adjusted to 85-95 DEG C, and the enzyme is completely inactivated by keeping for 10-15 min; (5) fermentation; (6) post-treatment, the fermentation liquid after the fermentation is kept at 50-60 DEG C for 10-30 min, and then cooling, centrifugation, collection of supernatant, vacuum freeze drying or spray drying of the supernatant are carried out, so that the egg yolk phospholipid polypeptide powder is obtained. The natural compound rich in specific functional peptide segments and phospholipids is prepared through the two-step enzymolysis coupling fermentation process, can effectively promote the growth and development of the bone, significantly improve the learning, memory and cognitive ability, and can significantly promote the proliferation of osteoblasts.

The invention discloses a phenylboronic acid affinity deoxynojirimycin imprinted polymer nano-material as well as a preparation method and application thereof, and belongs to the fields of nano-material preparation and natural compound extraction and separation. Phenylboronic acid modified nano silica gel is used as a carrier, deoxynojirimycin is used as a template molecule, dopamine is used as a cross-linking agent, a polyethyleneimine hyperbranched strategy is further combined, a deoxynojirimycin molecularly imprinted polymer (MIPs-PEI) with boric acid affinity and pH response characteristics is constructed, and hyperbranched modification remarkably improves hydrophilicity and boric acid affinity sites of the material. The synthesized MIPs-PEI has good adsorption performance on deoxynojirimycin and can effectively enrich deoxynojirimycin in mulberry leaves, and the natural hypoglycemic alkaloid deoxynojirimycin is extracted in a green mode through the method.

The application belongs to the technical field of medicine, and discloses application of SERPINE1 protein or a coding gene thereof as a new target for hepatitis B virus treatment. The application first discloses that SERPINE1 promotes autophagy flux by directly combining PIKfyve protein, thereby significantly inhibiting HBV replication and HBsAg expression. Based on this, the application establishes a multi-dimensional target verification platform, and first discovers that a natural compound, betulinic acid, is a high-affinity agonist (KD=1.46 µM) of SERPINE1. In-vivo and in-vitro experiments prove that betulinic acid can effectively reduce the levels of HBsAg and HBV DNA, and has good safety. In addition, betulinic acid and entecavir show a synergistic antiviral effect (CI<1). The application provides a new target and treatment strategy for realizing functional cure of hepatitis B.

Disclosed is a hair dye base composition comprising an improved basifying agent, which improvement is embodied in the overall reduction of ammonia compounds, thereby advantageously minimizing the problems associated with ammonia compounds. The composition provides a satisfactory hair dyeing covering power without malodors or damage to the scalp that are typically caused by ammonia compounds, while being free of ingredients known to cause skin damage problems, such as ethoxylated compounds, PEG and derivatives thereof, and silicones, since the composition contains a high percentage of natural compounds. Therefore, the composition of the present invention is significantly healthier for customers / consumers and hairstylists using the composition, while maintaining high performance of hair dyeing. Also disclosed are multi-compartment kits comprising the compositions, dyes and oxidizing components, as well as their use in permanent hair dyeing. Also disclosed is a process for producing the hair dye base composition, and a hair dyeing method comprising applying the composition to the hair.

The invention discloses a ferulic acid derivative and a preparation method and application thereof.The ferulic acid derivative is a compound shown in the formula (I) or pharmaceutically acceptable salt of the compound, and the compound is a natural compound extracted from rubus corchorifolius stems, has anti-inflammatory activity and anti-tumor activity and has the potential of being developed into anti-inflammatory or anti-tumor drugs. Or as a lead compound.

The invention discloses a tetrahydrofuran lignan compound as well as a preparation method and application thereof, the tetrahydrofuran lignan compound is a compound shown as a formula (I) or pharmaceutically acceptable salt thereof, the compound is a natural compound extracted from rubus corchorifolius stems, has anti-inflammatory activity and anti-tumor activity, and can be used for preparing a medicine for treating tumors. The compound has the potential of being developed into anti-inflammatory or anti-tumor drugs and can be used as a lead compound.

The present application relates to the application of ocanin in the preparation of anti-tumor drugs, and the ocanin is used in the anti-tumor drugs alone or in combination with 5-fluorouracil, and the concentration of the ocanin used in the preparation of anti-intestinal cancer drugs is 20-500 uMol. It is found that the ocanin can inhibit the growth of intestinal cancer cells in vivo and in vitro, and the naked mice have good tolerance to the natural compound. The ocanin has better anti-intestinal cancer effect than other extracts of goldeneye and devil's needle, and has better anti-cancer effect and smaller toxicity than the current conventional intestinal cancer drug 5-fluorouracil. Therefore, the ocanin can be developed into a new anti-cancer drug.

The invention belongs to the technical field of medicines, and particularly relates to tripterine-loaded nano-particles as well as a preparation method and application thereof, and the tripterine-loaded nano-particles (Cel-GDNPs) comprise ginger exosome (GDNPs) and tripterine (Cel) encapsulated in the ginger exosome. According to the invention, the Cel is used as a therapeutic drug, and the adopted carrier GDNPs has anti-inflammatory activity, so that when the Cel and the GDNPs are used for treating inflammatory diseases such as colitis and the like, the Cel and the GDNPs can play a multi-component synergistic therapeutic effect, and compared with the single use of the Cel or the GDNPs, the expression of proinflammatory factors (such as TNF-alpha, IL-1beta and IL-6) can be more effectively inhibited, so that the synergistic therapeutic effect is realized; a new strategy is provided for developing a novel natural compound preparation.

The invention belongs to the technical field of biological medicines, and particularly relates to a daphniphyllum calycinum extract compound, an extraction method thereof and application of the daphniphyllum calycinum extract compound in preparation of a medicine for preventing or treating non-alcoholic fatty liver diseases. A natural compound with a novel structure is obtained from daphniphyllum calycinum for the first time, a preparation method of the compound is provided, activity research is carried out on the compound, and experimental data shows that the compound is low in cytotoxicity and has no obvious cytotoxicity to normal cells, and the compound can be used for preparing the compound by inhibiting expression of fatty acid synthetases ACC, ACLY and FAS. The compound inhibits the de novo synthesis of fatty acid in cells, thereby inhibiting the accumulation of lipid droplets in the cells and reducing the lipid level in the cells, and has wide application prospects in the field of preparation of drugs for preventing or treating non-alcoholic fatty liver diseases.

The present application relates to the technical field of biological control of agricultural pests, and particularly relates to a method for improving pathogenicity of Metarhizium rileyi CQMa421 to Nilaparvata lugens by using carbon-hydrogen compounds on the body surface of the brown planthopper. The method for improving pathogenicity of Metarhizium rileyi to Nilaparvata lugens comprises mixing an effective dose of n-undecane liquid agent with the Metarhizium rileyi. The present application adopts the mixed use of n-undecane and Metarhizium rileyi, shortens the median germination time of Metarhizium rileyi, accelerates the initial process of infection, improves the pathogenicity to Nilaparvata lugens, and is conducive to rapid pest control. The n-undecane is a natural compound carried by insects, and the compound is safe to the environment and non-target organisms, and meets the development needs of green agriculture. The method only needs to simply mix the existing Metarhizium preparation with n-undecane before application, without the need to change the existing application equipment and process, and is easy to popularize and apply.

The invention aims to provide a novel application of a known natural compound. By screening and researching small molecular compounds derived from medicinal and edible substances, the fact that the original aubergine A has the activity of resisting chikungunya virus infection is found. Research results show that the original aubergine A can inhibit the infection process of chikungunya virus in a cellular level and an animal model, and shows relatively low cytotoxicity in an effective dose range. The invention provides the application of the protoaucoside A in preparation of the medicine for resisting chikungunya virus infection, provides a new technical scheme for prevention and treatment of chikungunya virus infection, and has potential application value.

The invention provides application of kestose in preparation of a medicine for treating heart failure related diseases, and belongs to the technical field of biological medicine. The prominent application value of the natural compound kestose in prevention and treatment of heart failure related diseases is disclosed for the first time. The traditional Chinese medicine composition has the direct effects of remarkably improving cardiac insufficiency and relieving myocardial injury; in the aspect of product application, a brand-new choice of a heart failure treatment medicine derived from a natural product is provided for clinic, the medicine has a good myocardial protection effect, a solid foundation is laid for developing a targeted treatment strategy aiming at a specific pathological link of chronic cross-induction, and the application has a wide application prospect. Important theoretical significance and wide clinical application prospects are realized.

The present invention provides methods for controlling gene expression of gene 5 engineered organisms using inducer compounds. The invention also relates to genetically modifying microorganisms by using natural and / or non-natural inducer compounds, such as carbon compounds and / or nitrogen-containing compounds, such as sugars, saccharide analogs, nucleotides, and the like, to develop efficient gene regulatory mechanisms. The present invention also describes gene expression regulation and 10 modulation by the use of a unique natural compound guanidine, alone or in combination with other inducer compounds, thereby achieving efficient gene expression control.

The application provides a method for preventing and controlling pear ring rot by using tyrosol, and the pear ring rot is prevented and controlled by spraying tyrosol aqueous solution externally; the concentration of tyrosol in the tyrosol aqueous solution is 1-5 mg / ml. The application takes 'Dangshan' pear as experimental material, and proves that tyrosol has a significant inhibitory effect on pear ring rot fungus through in-vitro antibacterial experiment and ring rot fungus inoculation experiment, can effectively inhibit the development speed of ring rot fungus spots, reduces the occurrence and spread of diseases, and thus reduces the invasion of ring rot fungus on plants. Tyrosol is a natural compound in plants, has low toxicity, and when applied to the prevention and control of pear ring rot, can enhance the defense mechanism of the plants, improve the natural resistance of the plants to ring rot, simultaneously reduces or replaces the use of chemical pesticides, and reduces the potential risks to the environment and human health.

This invention discloses a green and efficient bioreduction method for quinone-structured natural compounds used in textile dyeing. This method utilizes biosensor-regulated electrode microbial communities of *Shewanella putrefactiveis* and *Saccharomyces cerevisiae* as whole-cell biocatalytic reduction staining conditions. It constructs an electron transport system centered on cable bacteria and redox factors, and a dispersed staining system of quinone-structured natural compounds acting as electron acceptors. This provides a green and efficient bioreduction method for quinone-structured natural compounds used in textile dyeing. Electrons generated by biosensor-regulated electrode microbial communities are directly transferred between microbial species to cable bacteria and redox factors, and then efficiently transferred to highly dispersed natural compounds acting as electron acceptors, resulting in novel structures with dyeing properties.

The application discloses a new antibacterial compound and a preparation method and application thereof, and the new antibacterial compound is obtained by fermenting endophytic fungi (Epicoccum latusicollum) under stress of pathogenic fungi (Fusarium oxysporum), filtering, discarding mycelia, enriching the fermentation liquor by using a macroporous adsorption resin, and adopting high performance liquid preparation separation, and the chemical name of the new compound is (6E,8E)-13-acetoxy-3-hydroxy-8,10,12,14-tetramethylhexadeca-6,8-dienoic acid. The new compound obtained by fermenting endophytic fungi under stress of pathogenic fungi has significant antibacterial activity, and the application provides a scientific basis for mining endophytic fungi antibacterial natural compounds based on stress effects.

The scheme discloses an alkaloid compound with 14, 21 oxygen bridge structures and a preparation method and application thereof in the field of traditional Chinese medicine extraction and separation. The ethyl acetate extraction part of ethanol extracts of toad skin is repeatedly separated and purified by combining various chromatography technologies; 1D and 2D nuclear magnetic and high resolution mass spectrometry are used to identify the structures of two new compounds, and the molecular formula and structure formula of the two new compounds are deduced. In vitro pharmacodynamic studies show that the two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 The two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 values are 0.92 μM and 10.91 μM respectively. The present application is helpful for the development and utilization of natural compounds from toad.

The invention belongs to the technical field of separation and extraction of natural compounds, discloses a method for extracting and purifying pinostrobin from pigeon pea leaves and application of the pinostrobin, and particularly discloses a pinostrobin extraction method which comprises the following steps: mixing a pigeon pea leaf extract with normal-phase silica gel to obtain mixed silica gel, and performing column chromatography separation to obtain pinostrobin, an elution solvent used in the column chromatography separation comprises normal hexane and ethyl acetate. According to the method provided by the invention, the pinostrobin with high purity (98.72%-99.25%) can be simply and quickly obtained, and the method is high in production efficiency, easy to operate and easy for large-scale production. Meanwhile, the solvent used in the steps of extraction, column chromatography and the like is single, and the solvent can be recycled after distillation, so that the production cost is remarkably reduced. The extraction method can be used for preparing feed additives and medicines, and has a wide application prospect.

The application discloses a sesquiterpene dimer and a preparation method and application thereof, and belongs to the technical field of natural compound extraction. According to the preparation method, dried sesquiterpene dimers are extracted by refluxing with 95% ethanol, and then petroleum ether and ethyl acetate are used for extraction to obtain an extract, which can be obtained by column chromatography and semi-preparative high performance liquid chromatography, so that the method is simple, time and labor are saved, and the yield is higher. The obtained sesquiterpene dimers have high anti-neuritis activity, and have important application prospects for developing new drugs for treating senile dementia.

The invention discloses the technical field of animal cell culture and cell culture meat, and provides a food-grade natural compound combination method for promoting efficient myogenic differentiation in vitro. According to the method disclosed by the invention, a combination of xanthophyll and arachidic acid is added into a basic myogenic differentiation induction culture medium to form a myogenic differentiation promoting culture medium. According to the myogenic differentiation culture medium system, the myogenic differentiation efficiency of the myogenic cells can be improved from about 23% to 45% or above, the myogenic fusion efficiency can be improved from about 18% to 33% or above, the problems that the in-vitro differentiation efficiency of the myogenic cells is low, the content of key proteins such as myosin is insufficient and the like are effectively solved, and the myogenic differentiation culture medium system is suitable for large-scale popularization and application. And a feasible scheme is provided for large-scale muscle fiber production of cell culture meat.

The application discloses a new Sorbicillinoids compound and an extraction and separation method and application thereof, relates to the technical fields of natural compound extraction, separation and natural medicine chemistry. The application takes Trichoderma reesei fermentation liquor as raw material, extracts a new Sorbicillinoids compound from Trichoderma reesei metabolites, and names the compound as Trichosorbicil. The molecular formula of the Sorbicillinoids compound Trichosorbicil is C 25 H 28 O7, and a structural formula is as follows: The compound Trichosorbicil has relatively significant in-vitro anti-tumor activity, and provides a basis for further developing in-depth pharmacological and clinical research and developing a new anti-tumor drug with high curative effect and small side effect.

The invention belongs to the field of biological medicines, and relates to application of isohesperidin or pharmaceutically acceptable salts thereof in preparation of medicines for preventing and / or treating obesity or related metabolic syndromes thereof. The invention finds that the natural compound isohesperidin can obviously induce adipocyte browning and improve the expression level and the cell oxygen consumption rate of uncoupling protein 1, so that the weight gain is effectively inhibited and the glucose homeostasis and the insulin sensitivity are improved in an obesity mouse model, and meanwhile, the isohesperidin can be used for inhibiting the adipocyte browning. No obvious cytotoxicity and animal toxicity are observed in an effective dose of isohesperidin, so that the isohesperidin has better safety and is expected to be used for preventing and / or treating obesity or related metabolic syndromes thereof.

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The invention discloses a screening method and application of natural small molecules for inhibiting growth of Aspergillus carbonniger and synthesis of ochratoxin A, and relates to the technical field of bioinformatics. The invention particularly provides an application of coumarin or coumarin derivatives in preparation of a preparation for inhibiting growth of aspergillus carbonus niger and / or synthesis of ochratoxin A. The invention further provides a preparation method of the coumarin or the coumarin derivatives. According to the invention, SE of Aspergillus carbonus is taken as a target spot, small molecules with potential inhibitory activity are rapidly screened from a natural compound library by using a virtual screening technology, and experiments prove that coumarin and specific derivatives thereof not only can effectively inhibit the growth of Aspergillus carbonus, but also can efficiently inhibit the biosynthesis of OTA, so that the coumarin and specific derivatives thereof can be used for preparing drugs for inhibiting OTA. A theoretical basis and technical support are provided for the application of the strain in the postharvest preservation of agricultural products.

An aquatic liposome encapsulating a natural compound is provided, wherein an average particle size (a median particle size) of the aquatic liposome encapsulating the natural compound ranges from 80 nm to 200 nm. A manufacturing method of an aquatic liposome encapsulating a natural compound is provided and includes performing an ultrasonic oscillation after mixing the aquatic liposome and the natural compound, so that the natural compound is encapsulated in the aquatic liposome. Experiments are conducted to prove that the aquatic liposome encapsulating the natural compound could effectively enter microglia and retinal pigment epithelium cells to relieve the inflammatory response and hinder the apoptosis.