The invention discloses compound natural plant hair loss preventing liquid and a preparation method and application thereof in shampoo liquid. The compound natural plant hair loss preventing liquid comprises the following raw materials: yerbadetajo herb, tamariskoid spikemoss herb, parslane herb, raw sessile stemona root, Japanese honeysuckle stem, raw tuber of multiflower knotweed, raw astragalus, densefruit pittany root-bark, frostbitten mulberry leaf, angelica dahurica, chebule flesh, thick dried rehmannia, mume fruit, dried ginger, cochinchnese asparagus root, root of red-rooted salvia, aleppo gall and pomegranate rind. The preparation method comprises the following steps of: mixing the raw materials, and decocting in water to obtain decoction; removing impurities from the decoction, concentrating to form paste, and cooling to obtain a pasty substance; dissolving the pasty substance by using ethanol fully, standing, removing insoluble substances, and concentrating the obtained solution until the content of the ethanol is lower than or equal to 3 percent to obtain the compound natural plant hair loss preventing liquid. The compound natural plant hair loss preventing liquid is reasonable in combination of the raw materials, has the effects of promoting blood stasis, removing blood stasis, warming meridian, removing obstruction in channels, inhibiting bacteria and diminishinginflammation, can promote blood circulation of hair and has the effects of hair loss prevention, hair growth and hair nourishing.