37 results about "Cel" patented technology

At the bottom of a column (COLi) of memory cells (CEL) of the SRAM type with five portless transistors, there is placed an additional cell (CLS), with a structure identical to the cells (CEL), which makes it possible to write and read a datum in a memory cell (CEL) of the column without using a read amplifier.

The disclosure includes assays and methods for screening for risk of Down syndrome and/or trisomy 21 in a fetus. The assays and methods comprise determining the level of at least one biomarker selected from mucin 13 (MUC13), bile salt-activated lipase (CEL), dipeptidyl peptidase 4 (DPP4), carboxypeptidase A1 (CPA1), amyloid precursor protein (APP) and tenascin-C (TNC-C) polypeptides in a test biological sample from a pregnant subject, wherein a decreased level of MUC13, CEL, DPP4, and/or CPA1 polypeptide and/or an increased level of APP and/or TNC-C polypeptide in the test biological sample compared to a corresponding reference biomarker polypeptide level indicates an increased risk of Down syndrome or trisomy 21 in the fetus. The disclosure also includes assays, compositions, immunoassays, and kits for performing the methods disclosed herein.

The present invention provides an image generation system and program that apply shading for producing a cel animation type of image, with a reduced processing load. Brightness information is set for each pixel of a two-dimensional image that is obtained by perspective transformation of a three-dimensional object, based on brightness information set for that three-dimensional object, the two-dimensional image is divided into a plurality of areas based on the thus-set brightness information, and stepped shading is applied in units of the thus-divided plurality of areas. This brightness information could be set beforehand or it could be calculated in real-time, based on a given condition. The system could also comprise means for determining the pixels to be rendered by comparing the brightness information of each pixel with a given threshold value, then performing adjustment processing on the color information of the pixels to be rendered, based on color information that acts as a reference. This threshold value can be varied in accordance with the number of steps of shading, and adjustment processing can be performed a plurality of times on color information of pixels to be rendered.

An expansion tank (10) for a vehicle cooling system (18) of an engine using a liquid coolant (16) includes a tank body (12) defining a first volume (V1) containing coolant (16), wherein the coolant defines a variable coolant elevation level (CEL) within the tank body. The tank body (12) also defines an upper volume (20) containing air. A bladder (14) is disposed in the tank body (12) and defines a second volume (V2) containing air. The bladder (14) includes a flexible membrane (36) actuated by an actuator (46). When the engine is stopped or is below a predetermined temperature, the flexible membrane (36) is moveable to a first position (FP) which lowers the coolant elevation level (CEL), and when the engine is started or reaches a predetermined temperature, the flexible membrane (36) is moveable to a second position (SP) which raises the coolant elevation level. A communicating line (38) is in fluid communication between the upper volume (20) and the second volume (V2) to fluidly communicate air therebetween.

The invention discloses a method for testing the toxicity of high algae-laden aquatic organisms. The method comprises the steps that saccharomyces cerevisiae is cultured in a solid medium containing 5% of YPD and 1.5% of agar firstly, after clone is grown out, a YPD fluid medium of 5% is inoculated with monoclone, and overnight shake cultivation is conducted based on 180-250 rpm and 25-58 DEG C; phosphate buffer is added to a culture solution with the OD value of 1.5-1.8 to obtain a blank sample A, a high algae-laden water sample is added to the culture solution with the OD value of 1.5-1.8 to obtain a detection sample B, and the two samples are placed on a mixer for oscillation lasting 2 h; cell washing is conducted on the sample A and the sample B twice by means of PBS, and cell suspension with the concentration of 1*105-1*106 cel ls/mL is prepared; the sample A and the sample B are both added to Annexin V-FITC of 5 mug/mL to be evenly mixed, and then propidium iodide of 5 mug/mL is added to be evenly mixed; reaction is conducted at the room temperature away from light for 5-15 min; flow cytometry detection is conducted within 1 h; the proportion of dead cells, the proportion of living cells and the proportion of apoptotic cells are detected by means of flow cytometry; the apoptosis rate and the death rate of the sample B are compared with those of the blank sample A, so that water toxicity is judged. Early warning technical support is provided for high algae-laden water pollution, so that high water supply quality is guaranteed.

Ahsa-miR-489detection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-489detection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, anexogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum/plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5nmol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex for 15s; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribemiRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable thresholds and reference lines are set for result analysis.

The invention discloses a hierarchical porous triazinyl rare earth complex {[CeL(H2O)2].2H2O}n nanomaterial and a preparation method and application thereof. A gel-thermal aging process is adopted to ultrasonically prepare the hierarchical porous metal-organic frametwork complex {[CeL(H2O)2].2H2O}n nanomaterial; the material can modify an electrochemical working electrode to prepare a chiral triazinyl rare earth complex nanomaterial sensor; and a three-electrode system can be adopted to conveniently test the content of (R)-(+)-1-phenethylamine and (S)-(-)-1-phenethylamine enantiomers.

The invention discloses a human HLA region gene copy number variation detection method. The method includes: firstly, acquiring human tissues, blood or other body fluid genomes as samples, hybridizing the samples with CytoscanHD chips, washing the chips after hybridization is finished, and performing data collection in a data collection system of a GCD3000 system to generate CEL files; secondly, inputting the CEL files into CHAS software to perform primary quality control, importing Cychp files generated by the chips qualified in quality control into the CHAS software, and screening out a target marker according to three indexes including copy number characteristics, marker quantity and CNV gain and CNV loss fragment sizes. By precision of the CytoscanHD chips in detection of copy number variation, a direct and simplified analysis process of the CHAS software and specific setting of CHAS software intervals, accuracy in HLA region gene copy number variation detection is greatly improved, obtained data are higher in reliability and trueness, and prevention of habitual abortion and prenatal diagnosis are guaranteed significantly.

The invention discloses a hot metal square thermal analysis sample cup special for high-carbon equivalent. The hot metal square thermal analysis sample cup comprises a cup body, a thermocouple and a chilling agent, wherein the thermocouple is fixed in the cup body, the chilling agent is fixed on the bottom surface of the inner cavity of the cup body and composed of the following components of by weight, 7-8 parts of tellurium, 0.5-1 part of aluminum, 0.5-1 part of titanium and 1-2 parts of sulfur, and the above components are uniformly mixed after making 0.5-1.5 mm particles to form the chilling agent. The chilling agent in the invention can promote that the molten iron whether it is hypoeutectic or hypereutectic and whether it is subjected to inoculation treatment can be solidified by white mouth, and shows a long eutectic stop platform on the cooling curve, thereby obtaining accurate CEL, C and Si values.

The invention discloses an immortalization chicken embryo hepatocyte line as well as a preparation method and application thereof. The immortalization chicken embryo hepatocyte line CEL-hMRP18S-2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 6, 2016; the preservation number is CGMCC NO.12333; the preservation address is Microbiology Research Institute of China Science Academy, #3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The obtained immortalization chicken embryo hepatocyte retains main characteristics and main functions of the primary generation of chicken embryo hepatocyte; the immortalization chicken embryo hepatocyte can be amplified and cultured without limit in vitro; the hepatocyte line still has high activity after subculturing for 100 generations in vitro, can retain differentiation and proliferation and is free of senescence and apoptosis; when the immortalization chicken embryo hepatocyte line is applied to proliferation of type I aviadenoviruses, the preparation efficiency of virus antigens can be obviously improved.