128 results about "Total particulate matter" patented technology

The invention discloses an additive which can reduce harmful components in smoke and the preparation method. The invention is mainly characterized in that the materials include jasmine tea, liquorice, Beta-cyclodextrin, polyethylene glycol, honey, lactic acid and glyceryl triacetate and the materials are processed into cigarette filter additive, and then are evenly sprinkled on the polypropylene or acetate fiber cigarette filter rod and at last are rolled into a cigarette; the weight of the additive is 0.5-5% of the weight of the cigarette filter rod. Compared with a reference cigarette, the cigarette filter rod additive is balanced in tea aroma, flower fragrance, cigarette aroma and drug aroma and can improve the taste of the cigarette, enabling the cigarette to have balanced aroma, comfortable aftertaste and slender and soft smoke; the invention also reduces total particulate matter in the cigarette by 3.0-5.6%, tar by 2.9-6.6%, carbon monoxide by 2.5-10% and nicotinamide by 0.9-1%, and effectively reduces peppery irritation to the respiratory system of the smoker from the smoke, reduces respiratory diseases as well as harm to the smoker.

The invention provides a determination method of volatile organic acids in cigarette mainstream smoke. According to the determination method, the content of the volatile organic acids in total particulate matters of the cigarette mainstream smoke is determined by adopting vibration extraction and a gas chromatography-mass spectrometry technology. The determination method particularly comprises the following steps: (1) preparing a standard solution; (2) pretreating a sample; (3) qualitatively detecting the sample; (4) quantitatively detecting the sample; and (5) determining the content of the actual sample. According to the determination method of the volatile organic acids in the cigarette mainstream smoke, a standard sample addition method is used for qualifying and an internal sample standard curve method is used for quantifying; the recycling rate of the method is 97.5%-110.3%; the relative standard deviation (RSD%) of the method is 1.2%-8.5%; and the determination method has good sensitivity, accuracy and precision. Meanwhile, the method adopts the vibration extraction and a sample can be directly fed without deriving; and the determination method is simple and convenient to operate, and rapid in analyzing speed and is suitable for the determination of a large batch of the samples.

The invention provides a reconstituted tobacco paper base and a method for improving quality of the reconstituted tobacco paper base by adding wood pulp fiber. The method for improving quality of the reconstituted tobacco paper base by adding wood pulp fiber includes steps of impregnating for extracting tobacco stem and tobacco powder, grinding, pulping, sheeting, pressing and drying. Wood pulp fiber accounting for 3-15% of tobacco material pulp by weight percent is added into the tobacco material pulp in the sheeting step. Researches show that tensile index, tear index and permeability of the reconstituted tobacco paper base with the added wood pulp fiber are improved evidently, overall absorbent property of the reconstituted tobacco paper base is improved, contents of tar, CO and total particulate matter in the sheeted paper base are lowered evidently, tar reduction and harm reduction for reconstituted tobacco are better played, sensory evaluation of cigarettes made by applying the sheeted paper base to cigarette production is improved comprehensively, and technical support for improving the quality of the reconstituted tobacco is provided.

The invention discloses an in-vitro cell micronuclei method for detecting total particulate matters of main stream smoke of cigarettes. The method comprises the steps of seeding cells on a 96-hole plate for cigarette smoke contamination, directly fixing, dyeing, and feeding the cells into a high-connotation imaging system for detection; setting fluorescence intensity parameters of main nucleuses and the micronuclei of the cells according to the difference between a fluorescence value of cell nucleuses and a background fluorescence value, measuring the sizes of the main nucleuses and the micronuclei of the cells so as to set nucleus diameter parameters, and setting distance parameters according to distances between the cell nucleuses; performing analysis on images according to the setting, and selecting the number of binucleated cells with the micronuclei and the total number of the binucleated cells from a result lead-out column so as to calculate the micronucleus rate. According to the method, the number of required cells is small, the cells are not need to be used, and micronuclei sheets are not required to be manufactured; furthermore, the in-vitro cell micronuclei method is high in detection speed, large in sampling quantity and high in accuracy.

The invention discloses a method for analyzing the main stream smoke of a cigarette, which comprises the following steps of: a) smoking the cigarette with a filter tip, and collecting smoke total particulate matters by a filter disc; b) after finishing the smoking, putting the filter disc and the filter tip into an extraction liquid, and keeping the filter disc and the filter tip in the extraction liquid for more than 30 minutes, wherein the extraction liquid is isopropanol; c) concentrating the extraction liquid; d) performing gas phase chromatographic detection and mass spectrometric detection; and performing the steps from a) to d) again, wherein the extraction liquid used in the step b) is one of four non-polar solvents of n-hexane, cyclohexane, carbon dichloride or carbon tetrachloride. Compared with the prior art, the analysis method provided by the invention adopts the isopropanol and the n-hexane as the extraction liquids (extraction solvents), the isopropanol has a high extraction rate for compounds with medium polarities or strong memories, the n-hexane has a high extraction rate for low-pole or non-polar compounds, in particular for substances of benzene, homologues of the benzene, and the method can inspect most of the components of the main stream smoke trapped by a Cambridge filter disc.

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

The invention relates to a sampling device and a determination method of total particulate matters in exhaust gas. The sampling device comprises a combined heating and sampling gun, a glue gas condenser, a flue gas buffer, a CPM-F filter membrane clamp, a flue gas drier, a flow sensor, a flow automatic tracking and adjusting device and an exhaust device which are successively connected through a connecting pipeline. The sampling device and the determination method provided by the invention are suitable for determining total particulate matters in high humidity exhaust gas in a low concentration FPM emission level and can accurately evaluate the actual emission level of the particulate matters in the high humidity exhaust gas, in particular, the emission condition of fine particulate matters.

The invention relates to a method for detecting atomization homogeneity of an electronic cigarette atomizer. A linear smoking machine is adopted to smoke electronic cigarettes of the same specification and the same type in an ISO smoking mode, 44 mm Cambridge filters are used for trapping total particulate matter of smoke release matter after an electronic cigarette is smoked by 50 times continuously, an absorption bottle is connected between a catcher and a smoking needle cylinder, and 20 mL isopropyl alcohol extraction agents with 0.2-0.5 mg / mL n-heptadecane as internal standard substance is accurately added in the absorption bottle. After smoking, the absorption liquid in the absorption bottle is used for extracting nicotine in the Cambridge filters, and the gas chromatographic method is adopted to measure the nicotine content in the extraction agents according to the GB / T 23355-2009. The nicotine content in the smoke release matter of all electronic cigarette samples is calculated to obtain the SD, and the value of the SD responds the atomization homogeneity of the electronic cigarette atomizer in the batch. The method can quantitatively research the atomization homogeneity of the electronic cigarette atomizer, and is a practical and scientific method for estimation on the atomization homogeneity of the electronic cigarette atomizer.

The invention relates to a detection method for the cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure, belonging to the technical field of safety evaluation of cigarette smoke. The detection method provided by the invention comprises the following steps of: firstly, setting cigarette suction parameters to carry out suction on a cigarette; diluting the smoke by clean air with the flow speed of 0-1000 mL / min and transferring the diluted cigarette full smoke into a full-smoke exposure bottle disclosed in the patent with the patent number of ZL201120063251.6 to come into contact and exposure with suspension cells in the bottle, wherein the exposure time is 5 min; after the exposure, carrying out neutral erythrocyte toxicity test or MTT (Methyl Thiazolyl Tetrazolium) cytotoxicity test according to a regular method; analyzing a dosage reaction relation between smoke exposure amount and cytotoxicity according to the following formula: full-smoke dilution multiple=( flow speed of smoke + flow speed of clean air) / flow speed of smoke; smoke exposure amount under exposure of 10 mL of a cell suspension solution for 5 min=TPM (Total Particulate Matter) amount of single cigarette / suction times of single cigarette*6 suctions / 10 mL; and representing the extent of cytotoxicity by a cell inhibition ratio CI. The detection method provided by the invention has the advantages of simplicity, feasibility, low cost and the capability of providing technical supports for the safety evaluation of cigarette smoke.

The invention discloses a method for simultaneously measuring 19 smoke incense ingredients in the main stream smoke of a cigarette. The method comprises the following steps of 1) after using a Cambridge filter to gather a total particulate matter in the main stream smoke of the cigarette, transferring the Cambridge filter into an extraction container, adding an internal standard solution, then adding ethyl alcohol to extract through vibration, and taking a liquid supernatant to obtain a smoke sample; and 2) detecting the smoke sample through GC-MS (Gas Chromatography-Mass Spectrometer), and detecting the contents of 19 smoke incense ingredients through an internal standard quantitative method. According to the method, a solvent extraction-gas chromatograph mass selective detector is adopted to simultaneously measure the contents of 19 smoke incense ingredients in the main stream smoke of the cigarette, and the method has the advantages of rapidness, high sensitivity, high selectivity and high accuracy, and is particularly suitable for measuring the contents of the smoke incense ingredients in the smoke of the cigarette.

A measuring method of absorption coefficients of total particulate matter and phytoplankton in the water relates to a measuring method of total particulate matter and components thereof. The invention aims to solve the problem that when measuring the absorption coefficient of total particulate matter at home and abroad, phycobilin and some pigments of eukaryotic cells can not be well extracted when methanol is used to extract for removing phytoplankton, and the absorption of non-pigment particulate matter and the inaccurate measurement of absorption coefficient are caused by using sodium hypochlorite to extract for removing phytoplankton. The method of the invention comprises the following steps: 1) preparing filtration film samples and a blank sample; 2) placing the samples in a spectrophotometer to measure the absorbance; 3) calculating the absorption coefficient of total particulate matter; 4) extracting filtration film samples with acetone to remove phytoplankton and retaining non-pigment particulate matter; 5) obtaining the absorbance of non-pigment particulate matter; 6) calculating the absorption coefficient of the non-pigment particulate matter; and 7) calculating the absorption coefficient of phytoplankton.

Methods for treating tobacco which result in the reduction of selected compounds from the tobacco smoke, and corresponding smoking articles containing this treated tobacco, are disclosed. In these methods, a solution containing a palladium salt is applied to the tobacco, preferably by spraying. The palladium salt preferably contains nitrate, chloride, acetate, citrate, gluconate, or salicylate as the anion. After application of the solution, the tobacco is preferably dried in an oven, by forced air, or by natural evaporation. The palladium in the treated tobacco results in the reduction in the level of polycyclic aromatic hydrocarbons, especially benzo(a)pyrene, from the total particulate matter in mainstream and sidestream smoke of the treated tobacco. The addition of mannitol and / or urea to the tobacco, and introduction of ventilation in a cigarette containing the treated tobacco, enhance the ability of palladium to reduce the level of polycyclic aromatic hydrocarbons in the smoke.

The invention discloses a filter tip additive material capable of improving the sensory characteristics of smoke and the application thereof. The filter tip additive material is characterized by being prepared by carrying a mixed solution of propylene glycol and sorbitol on a carrier which is a mixture of porous SiO2 and Al2O3 particles. A two-element or multi-element combined filter tip can be manufactured by adding the prepared filter tip additive material into a cigarette filter stick. The filter tip additive material prepared through the method contains a low-molecular hydrophilic moisture-retaining agent, moisture can be effectively retained for a long time and can be rapidly released when a cigarette is smoked, the moisture content in the smoke of the cigarette is increased, and the sensory quality of the cigarette is improved. Meanwhile, by means of the method, the content of harmful ingredients such as tar and total particulate matter in the smoke can be effectively reduced. In addition, the filter tip additive material is nontoxic, harmless and low in cost and has broad industrial prospects, and the preparation process is simple and easy to implement.

The invention relates to a method for preparing a cigarette mainstream smoke total particulate matter sample for biological detection, which belongs to the technical field of safety assessment of tobaccos and cigarettes. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection is improved based on the prior art, wherein the improved points comprise the following steps of: a, directly soaking a Cambridge filter which catches cigarette mainstream smoke total particulate matters in an organic solvent dimethyl sulfoxide, performing ultrasonic extraction for 20 minutes, filtering a mixture by using filter paper, subpackaging a filtrate to a 2 ml freezing tube, and reserving the freezing tube in an ultra-low temperature freezer at the temperature of 70 DEG C below zero for later use; and b, adding the organic solvent dimethyl sulfoxide at the speed of 10 mg TPM / ml at one time without setting a constant volume. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection has the advantages of simple and convenient operation steps, and capacity of truthfully reflecting the mutagenicity of the cigarette mainstream smoke total particulate matters.

The invention discloses a method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum. The method comprises following steps: (1) total particulate matters in smoke are collected by a fishtail cover and a filter disc; (2) the filter disc is placed in a methanol solution containing internal standard substances for extraction so as to obtain an extract; (3) the extract is filtered, and then is detected by a high performance liquid chromatography-tandem mass spectrometer; and (4) the content of caffeic acid is quantitatively analyzed by internal standard method. According to the method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum, pre-treatment is simple, separating effect is excellent, qualitative analysis is accurate, sample analysis time is short, and detection sensitivity is high.

The invention provides a smoking machine with a constant flow velocity. The smoking machine comprises a cigarette clamp holder, a total particulate matter catcher, a reversing valve, a gas flow detection control device, a vacuum pump and a mainstream smoke collecting opening, wherein the cigarette clamp holder is arranged on an upper panel; the total particulate matter catcher is connected onto a mainstream smoke pipeline at the back end of clamp holder; an organic glass cover used for collecting sidestream smoke is mounted on the upper panel; a sidestream smoke outlet is formed in the upper panel positioned in the glass cover; a sidestream smoke filter screen is additionally arranged at the sidestream smoke outlet, and the sidestream smoke outlet is communicated with a sidestream smoke blower and a sidestream smoke collecting opening; the total particulate matter catcher is mounted on the upper panel; the reversing valve, the gas flow detection control device and the vacuum pump are arranged on a bottom plate; a cleaning gas circuit interface is arranged on the reversing valve. The smoking machine with the constant flow velocity, provided by the invention, has the advantages that smoking flow quantity of a cigarette can be stabilized, the setting and control of the constant flow velocity can be achieved, a smoke passage can be cleaned timely, influences on experimental results are reduced greatly, the mainstream smoke and sidestream smoke can be collected in an expanded manner during cigarette burning, and analysis and detection to compositions of the mainstream smoke and the sidestream smoke can be achieved.

The invention relates to a method for detecting the mutagenicity of the total particulate matter of cigarette smoke, and belongs to the technical field of tobacco and cigarette smoke safety evaluation. The method is improved on the basis of an Ames test plate incorporation method; and the improvement points are that: a, the amount of histidine is 20 to 25 mg / ml, the amount of top layers is 2.5 ml, and the holding temperature of the top layer is 43 DEG C; b, the dosage range of the mutagenicity of the total particulate matter of the cigarette smoke is 25 to 500 mu g / vessel; and c, the positive substance of a strain TA98 is 2-aminofluorene of which the adopted dosage is 1 to 10 mu g / vessel; and the positive substance of a strain TA100 is sodium azide of which the adopted dosage is 0.15 to 1.5 mu g / vessel. The method has the advantages of simplified operation steps, reduced test period, consistent effect, obvious bacterial reverse mutation, reduced positive substance dosage, proper dosage range of selected total particulate matter of the cigarette smoke, and capability of detecting the mutagenicity of the total particulate matter of the cigarette smoke.

The invention discloses a method for determining benzoapyrene (B[a]P) in the main stream smoke of a cigarette by supercritical fluid chromatography (SFC)-gas chromatography-mass spectrometry (GC-MS). The method comprises the following steps: adding an internal standard substance and a cyclohexane solution into a filter element for capturing total particulate matters of the main stream smoke of the cigarette after the cigarette is smoked; performing ultrasonic extraction, separating and purifying the extract with SFC; cutting fraction containing B[a]P, and analyzing with GC-MS; and fixing the quality according to the area ratio of a target peak to an isotope internal standard substance peak. According to the method, trace to-be-determined ingredient in a complex substrate can be separated from an interfering substance, interference of other substances can be eliminated, and the detection accuracy can be improved. The method has the advantages of simple pre-treatment, high separation speed, accurate quantity, low detection limit and the like.

The invention relates to a method for measuring the main active ingredient paeonol of a traditional Chinese medicinal additive in cigarettes, The method comprises the following steps of: (1) extracting paeonol from a cigarette sample; (2) concentrating the extraction liquid till the extraction liquid is dry and obtaining residues; (3) dissolving the residues with methanol and adjusting the volumeto reach a set scale to obtain a test product; and (4) carrying out the HPLC (High Performance Liquid Chromatography) analysis to the test product. The invention has the advantage that the paeonol incigarette cut tobacco and total particulate matter of smoke can be fully extracted so as to realize rapid, accurate and quantitative analysis and determination.

The invention provides a sampling device and a sampling method for low concentration total particles in smoke of a thermal power plant, which is used for detecting emission of total particles in smokeafter improvement of ultralow emission or smoke plum treatment and detecting particle removing performance of equipment. The sampling device mainly comprises a sampling device, a condensing device, adehumidifying device, a metering device and a sampling pump that are arranged in sequence in a smoke flowing direction, wherein the sampling device comprises a sampling head and a heating sampling gun, the sampling head comprises a sampling nozzle and a first-level filter membrane, a second-level filter membrane is arranged between the condensing device and the dehumidifying device, the condensing device has a two-level serial structure, the two levels of condensing device are provided with a bath temperature control device separately, a strengthening phase change system is arranged between the two levels of condensing device and is used for adding vapor to smoke entering the latter condensing device, so as to make the smoke wetter and capture more total particles. The device can test total particles reasonably, accurately and comprehensively.

The invention provides an essence suitable for low-temperature non-combustion smoke products, a preparation method and an application thereof, and relates to the technical field of low-temperature non-combustion smoke products. The essence comprises components with antitussive and expectorant effect and the components with antitussive and expectorant effect is prepared from at least one of foliumArtemisiae Argyi oil, daurian rhododendron oil, oleum viticis negundo, alpha-asarone, Germacrone, P-Cymene, guaiacol sulfonic acid potassium, acetylcysteine, isofraxidine, esculetin, ligustilide, stachydrine, ligustrazine, imperatorin, isoimperatorin, Praeruptorin A, Praeruptorin B, Praeruptorin C, hygrine, and Cuscohygrine. The antitussive and expectorant active ingredient is natural active ingredient, which can volatilize and transfer to the total particulate matter of the smoke at atomization temperature, enrich the smoke and enhance the mouthfeel, and at the same time is partly absorbed bythe human body, so that the low-temperature non-combustible smoking product has the function of regulating the respiratory system function to a certain extent.

The invention discloses a method for determining phenolic compounds in mainstream smoke of cigarettes by supercritical fluid chromatography (SFC), gas chromatography and mass spectrum (GC-MS). The method comprises the following steps that: after the cigarettes are smoked, supercritical fluid is used for extracting glass-fiber filtering discs which capture total particulate matters in the mainstream smoke of the cigarettes, the supercritical fluid chromatography is used for dividing extraction liquid into multiple sections of fractions, and every section of fraction is analyzed by the gas chromatography and the mass spectrum. The method disclosed by the invention has the advantages that the problem of limited peak capacity of GC-MS is solved, the purpose that the compounds among all sections after separation are basically not overlapped can be realized. The result is satisfactory, the quantity of the separated phenolic aroma components is much more than that of direct sampling analysis, a new method is provided for determining the phenolic aroma compounds in the mainstream smoke of the cigarettes, and has significance in evaluating the hazards of smoking on health and promoting research and development of low-hazard cigarettes.

The invention belongs to the method for measuring bioamine in main stream smoke of cigarettes. The method comprises the following steps: (1) preparation of a mixed series standard working : preparing the mixed series standard working solution with concentration gradient and containing an interior standard; (2) pretreatment of a sample: adding a cambridge filter gathered with total particulate matters of the main stream smoke of the cigarettes into 2% of a formic acid aqueous solution, and ultrasonically extracting for 30 minutes; (3) purification with solid-phase extraction column: enabling extract liquor obtained in the step (2) to flow through a mixed type cation exchange solid-phase extraction column, after eluting, blow-drying eluant under nitrogen, and redissolving with 2 mL of an acetonitrile aqueous solution to obtain sample purifying liquid; (4) carrying out HPLC-MS / MS measurement on the mixed series standard working solution containing the internal standard obtained in the step (2) and the sample purifying liquid obtained in the step (3) under the same conditions; and (5) result calculation: quantifying by using an internal standard method according to the ratio of the peak area of a targeted analyzed object to the peak area of the internal standard.

The invention discloses a method of detecting influence on early apoptosis of cells due to total particulate matters in cigarette smoke. The method includes the steps of: 1) pre-treating to-be-detected substances; 2) preparing a human lung fibroblast single cell suspension liquid; 3) calculating cell concentration of the human lung fibroblast single cell suspension liquid; 4) performing cell inoculation to the human lung fibroblast single cell suspension liquid; 5) grouping the to-be-detected samples; 6) setting detection dose of the to-be-detected samples; 7) preparing a to-be-detected sample culture substance; 8) incubating the to-be-detected samples; 9) collecting incubated products of the to-be-detected samples; and 10) calculating influence ratio of early apoptosis of cells. Through optimization of sample detection dose and correct selection on target cells, the method can accurately and effectively detect the influence on early apoptosis of cells due to the total particulate matters in cigarette smoke. As a complementary method for cytotoxicity detection for the total particulate matters in cigarette smoke, the method distinguishes the influence between necrocytosis and cell apoptosis of the product.

The invention discloses tar and harm reducing cellulose balls for cigarette filter tip and preparation thereof. Through mutual winding of cellulose fibers, mass ball-shaped cellulose is caked. The groups of the modified fibers in a cellulose forming ball process achieve the purpose of chemically adsorbing harmful components of the cigarette smoke; cellulose balls with high adsorption capability are added into an acetate fiber filter stick to effectively reduce the harmful components in the cigarette smoke; and the ball-shaped cellulose can effectively adsorb the total particulate matter in the cigarette smoke.

Systems, devices, and methods may control total particulate matter (TPM) production based on air flow rate and TPM density. TPM density is a TPM production rate per flow rate of gas through an aerosol-generating device. The systems, devices, and methods may measure, or sample, air flow during a puff or draw on an aerosol-generating device. The aerosol-generating device may, in turn, adjust the production of the TPM based on the measured air flow rate and a selected, or desired, TPM density, which may be pre-set in the device and / or subsequently set as desired.

The invention relates to a regional atmospheric particulate concentration target accessibility evaluation method. The related atmospheric environment quality protection problem can be effectively solved, and two index data including the local source contribution rate and the primary emission proportion of fine particulate matter in an atmospheric particulate matter source analysis result of a reference year area and one index data including the local source contribution rate of inhalable particulate matter are obtained; calculating the total particulate matter emission reduction amount by calculating and summing the particulate matter emission reduction amount in the region; the regional atmospheric particle concentration prediction formula is utilized to predict the particle concentrationin the target year, and the particle concentration prediction data is compared with the target value, so that regional atmospheric particle concentration accessibility evaluation is realized, and scientific protection of the regional atmospheric environment is promoted. The method is simple, easy to operate, stable and reliable, data are easy to obtain, the method can be effectively used for evaluating the accessibility of the regional atmospheric particulate concentration target, high practicability is achieved, and technical support is provided for strengthening environmental protection anddecision making.

The invention discloses a method for detecting the influence of total particulate matters in cigarette smoke on reactive oxygen secretion of cells. The method comprises the following steps: (1) pretreatment of to-be-detected total particulate matters in cigarette smoke; (2) preparation of monoplast suspension of human lung fibroblasts; (3) calculation of the cell concentration of the monoplast suspension of human lung fibroblasts; (4) cell inoculation of the monoplast suspension of human lung fibroblasts; (5) grouping of the to-be-detected total particulate matters in cigarette smoke; (6) setting of the detection dosages of the to-be-detected total particulate matters; (7) preparation of cultivated to-be-detected total particulate matters; (8) incubation of the to-be-detected total particulate matters; (9) addition of positive controls; (10) probe loading; (11) sample determination; and (12) calculation of reactive oxygen species. Through effective treatment of samples, optimization of the detection dosages and correct selection of target cells, the method is capable of accurately and effectively detecting the influence of the total particulate matters in cigarette smoke on reactive oxygen species in cells.

The application discloses a determination device of total particulate matter in smoke. The determination device comprises a particulate matter sampling head, a FPM filter membrane, a CPM filter membrane, and an exhausting host; the particulate matter sampling head, a heat-tracing sampling gun, a snakelike condenser, a condensate collecting bottle, a rear impact bottle, the CPM filter membrane, a super-pure water absorbing bottle, a silica gel drying bottle and the exhausting host are orderly connected; the condensate collecting bottle, the rear impact bottle, the super-pure water absorbing bottle and the silica gel drying bottle are arranged in a water bath; the FPM filter membrane is arranged in the particulate matter sampling head, the CPM filter membrane is filled in a CPM filter membrane cavity, the particulate matter sampling head and the heat tracing sampling gun are inserted into a flue, the exhausting host is started, the smoke enters the snakelike condenser to be condensed andcollected, and the collected condensate and CPM filter membrane are used for determining organic phase and inorganic phase condensable particulate matter and the particulate matter on the FPM filtermembrane, thereby obtaining the total particulate matter quantity; the synchronous collection and determination of the CPM and the FPM is realized, and the representative TPM drainage data is acquired.

The invention provides a method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke. The method comprises the following steps: providing a to-be-detected solution containing mainstream smoke particulate matters and an internal standard substance, wherein the internal standard substance is N-amyl-(3-methyl pyridine) nitrosamines; carrying out gas chromatography-thermal energy analysis on the to-be-detected solution to obtain a to-be-detected solution chromatographic curve, wherein the sample feeding mode of the gas chromatography-thermal energy analysis is pulse without shunting; calculating the content of specific N-nitrosamines in tobacco in the to-be-detected solution according to the to-be-detected solution chromatographic curve and a preset standard curve; and calculating the content of N-nitrosamines in tobacco in total particulate matters of mainstream smoke according to the content of specific N-nitrosamines in tobacco in the to-be-detected solution. Therefore, the method provided by the invention has high sensitivity and accuracy on the detection of the specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke.