Method for measuring main active ingredient paeonol of traditional Chinese medicinal additive in cigarettes

An active ingredient, paeonol technology, applied in the field of determination of paeonol, the main active ingredient of Chinese medicinal materials, can solve problems such as the difficulty of separation and accurate determination of paeonol

Active Publication Date: 2011-02-16
CHINA TOBACCO JIANGXI IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical components in tobacco are diverse and very complex. It is very difficult to separate and accurately determine the paeonol in the tradi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Determination of Paeonol in Cut Tobacco

[0018] a. Weigh 40g of shredded cigarette tobacco sample, add chloroform-absolute ethanol (1:1, V / V) 400mL to the flask, soak overnight at room temperature, extract ultrasonically for 15min, cool to room temperature, filter, and extract Wash the shredded tobacco and container 3 times with 3×50mL, and combine the filtrates.

[0019] b. Concentrate the obtained filtrate to dryness at 20kPa, dissolve the residue with 40mL of methanol, and dilute to 50mL to obtain the test sample.

[0020] c, HPLC analysis is carried out to obtained sample.

Embodiment 2

[0022] Determination of Paeonol in Cut Tobacco

[0023] a. Weigh 50g of shredded cigarette tobacco sample, add 450mL of chloroform-absolute ethanol (1:1, V / V) to the flask, soak overnight at room temperature, ultrasonically extract for 20min, cool to room temperature, filter, and extract Wash the shredded tobacco and container 3 times with 3×50mL, and combine the filtrates.

[0024] b. Concentrate the obtained filtrate to 23 kPa, dissolve the residue with 40 mL of methanol, and set the volume to 50 mL to obtain the test sample.

[0025] c, HPLC analysis is carried out to obtained sample.

Embodiment 3

[0027] Determination of Paeonol in Cut Tobacco

[0028] a. Weigh 60g of shredded cigarette tobacco sample, add 600mL of chloroform-absolute ethanol (1:1, V / V) into the flask, soak overnight at room temperature, extract ultrasonically for 30min, cool to room temperature, filter, and extract Wash the shredded tobacco and container 3 times with 3×50mL, and combine the filtrates.

[0029] b. Concentrate the obtained filtrate to dryness at 25 kPa, dissolve the residue with 40 mL of methanol, and dilute to 50 mL to obtain the test sample.

[0030] c, HPLC analysis is carried out to obtained sample.

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Abstract

The invention relates to a method for measuring the main active ingredient paeonol of a traditional Chinese medicinal additive in cigarettes, The method comprises the following steps of: (1) extracting paeonol from a cigarette sample; (2) concentrating the extraction liquid till the extraction liquid is dry and obtaining residues; (3) dissolving the residues with methanol and adjusting the volumeto reach a set scale to obtain a test product; and (4) carrying out the HPLC (High Performance Liquid Chromatography) analysis to the test product. The invention has the advantage that the paeonol incigarette cut tobacco and total particulate matter of smoke can be fully extracted so as to realize rapid, accurate and quantitative analysis and determination.

Description

technical field [0001] The invention relates to the determination of paeonol, the main active component of traditional Chinese medicinal materials, in particular to a method for determining the main active component of paeonol as an additive of traditional Chinese medicinal materials in cigarettes. Background technique [0002] The main active ingredient of traditional Chinese medicine Cortex Moutan is paeonol. Paeonol has significant pharmacological activities such as improving blood rheology, lowering lipids, inhibiting lipid peroxidation, anti-tumor, anti-inflammation, and analgesia, and has anti-arrhythmia, anti-atherosclerosis, improving microcirculation, protecting Ischemic tissue, enhancement of immunity, antibacterial effect and inhibition of skin pigment synthesis. Usually, the methods for detecting paeonol in medicinal materials are mainly high performance liquid chromatography and thin layer chromatography. The chemical components in tobacco are diverse and very...

Claims

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Application Information

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IPC IPC(8): G01N30/36G01N30/06
Inventor 廖堃蔡继宝周会舜张文惠李福龙
Owner CHINA TOBACCO JIANGXI IND CO LTD
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