Method of detecting influence on early apoptosis of cells due to total particulate matters in cigarette smoke

Abstract

The invention discloses a method of detecting influence on early apoptosis of cells due to total particulate matters in cigarette smoke. The method includes the steps of: 1) pre-treating to-be-detected substances; 2) preparing a human lung fibroblast single cell suspension liquid; 3) calculating cell concentration of the human lung fibroblast single cell suspension liquid; 4) performing cell inoculation to the human lung fibroblast single cell suspension liquid; 5) grouping the to-be-detected samples; 6) setting detection dose of the to-be-detected samples; 7) preparing a to-be-detected sample culture substance; 8) incubating the to-be-detected samples; 9) collecting incubated products of the to-be-detected samples; and 10) calculating influence ratio of early apoptosis of cells. Through optimization of sample detection dose and correct selection on target cells, the method can accurately and effectively detect the influence on early apoptosis of cells due to the total particulate matters in cigarette smoke. As a complementary method for cytotoxicity detection for the total particulate matters in cigarette smoke, the method distinguishes the influence between necrocytosis and cell apoptosis of the product.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, and in particular relates to a method for detecting the effect of total particulate matter in cigarette smoke on early apoptosis of cells. Background technique [0002] The concept of apoptosis was first proposed by Kerr and other three scientists in 1972. Although the final results of apoptosis and necrosis are very similar, their processes and manifestations are quite different. Apoptosis is an active process, which involves the activation, expression and regulation of a series of genes. It is not a phenomenon of autologous injury under pathological conditions, but is actively strived for to better adapt to the living environment. a dying process. For the detection of cytotoxicity of total particulate matter in cigarette smoke, the International Tobacco Scientific Research Cooperation Center CORESTA recommends the neutral red blood cell toxicity method,...

AI Technical Summary

Problems solved by technology

For the detection of cytotoxicity of total particulate matter in cigarette smoke, the International Tobacco Scientific Research Cooperation Center CORESTA recommends the neutral red blood cell toxicity method, but the neutral red blood cell toxicity method has certain defects

Images