103 results about "Sample culture" patented technology

The invention discloses a multi-sample culture device which comprises a base and a plurality of culture vessels. All the culture vessels are stacked, each culture vessel comprises a vessel body, a vertical barrel, a protruding block and a liquid injection pipe, a vessel top groove is formed in the top end face of each vessel body, a base top groove is formed in the top of the base, and the protruding block of the bottommost culture vessel is located in the base top groove. A rotary pipe penetrates through all the vertical barrels, the rotary pipe and the vertical barrels can relatively rotate, the outer side wall of the rotary pipe and the inner side walls of the vertical barrels are in sealed contact, and a liquid drainage groove is formed in the outer side wall of the rotary pipe. A liquid balancing hole communicated with the inside of the corresponding vessel body is formed in each vertical barrel, a cell filter screen and a liquid balancing one-way valve are arranged in each liquid balancing hole, and the lower end of each liquid injection pipe is communicated with the inside of the corresponding vessel body. The protruding blocks of the upper culture vessels are located in the vessel top grooves of the lower culture vessels. The multi-sample culture device has the advantages of being suitable for cell culture, testing and comparing, reasonable in spatial arrangement, stable in structure, convenient to operate, good in adjusting consistence in the experiment process and high in accuracy.

The present invention discloses a rapid online detection apparatus and a detection method for water body bacterial microorganisms. According to the present invention, based on optical scattering and absorption characteristics of bacterial microorganisms, a double-wavelength laser diode is adopted as a light source, an optical fiber array distributed in a spherical space is adopted as an optical signal receiving device, and CCD is adopted as an optical signal detector to research rapid online detection methods and systems for bacterial microorganisms in environmental water bodies, wherein a set of rapid online detection sample machine for water body bacterial microorganisms is developed, and rapid recognition detection on water body bacterial microorganisms is effectively achieved in the laboratory; and the method has characteristics of simple operation, no requirement of sample culture and pretreatment, rapid detection and high sensitivity, can be provided for achieving continuous, online and real time monitoring and classification of bacterial and microorganisms in the water body, and is suitable for rapid online detection of bacterial microorganisms in the nature water body, and complete and continuous monitoring pre-warning of bacterial microorganisms in the drinking water source water body.

A stirring system (1) is disclosed for use in a cell culture system which handles a large number of cell culture vessel assemblies (2). Each cell culture vessel assembly includes a platen (23) on which are disposed a plurality of stirring rods (25), and which is transversely movable across the top of the respective culture vessel to provide for stirring of one or more samples within the culture vessel. The stirring system includes a support (10) configured to receive plural cell culture vessel assemblies at predefined positions around an axis (z) and has a drive rotor (14) disposed adjacent the support, the drive rotor being mounted for eccentric movement about the axis (z) of the support (10) with respect to the support. The drive rotor has plural of drive connections (24) for selectively engaging respective platens (23) of culture vessel assemblies supported at the predefined positions so as to enable simultaneous stirring of samples in culture vessels whose platens are engaged with the respective drive connections. A drive

The invention discloses a portable cell incubator. The portable cell incubator comprises an incubator body provided with a sample culture area, and a circuit control device, wherein the circuit control device comprises a microcontroller, a temperature control device, and a humidity control device. The internal space of the incubator box is partitioned into a water cooling zone, an ultrasonic humidification water consumption zone and an ultrasonic humidification zone by a division plate; the temperature control device is used for setting different temperature values through a keyboard input device, and can adapt to temperature values required by culturing and preserving cell samples in different cases; and the humidity control device comprises a humidity sensor, an ultrasonic atomization humidifier and a humidity control circuit. In the incubator, a semiconductor chilling plate is used for raising and reducing temperature and humidification is realized by ultrasonic atomization, and control parameters of the culture area are regulated and controlled in real time by utilizing data measured by the sensor, so that constant temperature and constant humidity are realized; a lead storagebattery is adopted to supply electricity, so the incubator can steadily work for a long time; and the incubator has a smaller volume and light weight, is convenient to carry, and is suitable for remote transmission.

The invention provides a cavity-type dynamic-filling bioreaction device comprising a biological sample culture component, a nutrient solution flowing pipe and a power pump component. The biological sample culture component is provided with an in-out port communicated with the nutrient solution flowing pipe. The biological sample culture component comprises an upper cover body, a container body and a sample bearing frame, wherein the upper cover body is connected with the container body, a supporting ring is arranged on the inner wall of the container body, the sample bearing frame is arranged on the supporting ring, a supporting block is arranged on the inner side of the sample bearing frame, a sample bearing plate is arranged in a cavity formed by the supporting block and the sample bearing frame, and culture through holes are longitudinally formed in the sample bearing plate. The nutrient solution flowing pipe comprises an output pipe and an input pipe, wherein the output pipe and the input pipe are respectively connected with the power pump component.

The invention discloses a rotary adjustable alpha-source irradiation device which is characterized by comprising a bracket, a rotary platform erected on the bracket, a plurality of sample culture dishes arranged on the rotary platform, a liftable alpha-source irradiation mechanism erected on the bracket below the rotary platform, wherein the rotary platform is driven by a motor. The rotary adjustable alpha-source irradiation device is reasonable in design, the irradiation dose on the cell is adjusted by adjusting the height of the irradiation source, the rotation speed of the sample platform and the angle of a sample outlet, the fabrication cost is low, the irradiation device can perform the linear energy transfer (LET) irradiation in different doses, and different dosage rates, the irradiation dose is accurate and the irradiation controllability is good.

The invention discloses an in-situ culturing device for deep-sea microbes. The device comprises sample culturing boxes, culturing box placement cases and a placement case protection cover, wherein each sample culturing box comprises a box body, an upper locking cover and a lower locking cover; a sealing gasket, a filter membrane, a sealing gasket, the box body, a sealing gasket, a filter membrane and a sealing gasket are sequentially arranged between each upper locking cover and the corresponding lower locking cover; more than one sample culturing box can be accommodated in each culturing box placement case; more than one culturing box placement case can be accommodated in the placement case protection cover. The device has the advantages that the device can be directly arranged in deep sea, the natural environment of the deep-sea microbes can be maximally restored, and the deep-sea microbes can naturally grow; chemical substances can freely penetrate through the filter membranes, so that interaction between microbial communities can be ensured, and the culturability of the microbes is improved; the shortcoming of incapability of the conventional microbe culturing technology in simulating or completely simulating the natural conditions of existence for marine microbes is overcome, and the bottleneck in researches on nonculturable microbes is broken.

The invention discloses a flammulina velutipes spawn liquidation preparing method which enables solid spawns to be directly prepared into liquid spawns in an expanding mode. The flammulina velutipes spawn liquidation preparing method includes the following steps: (1) preparing a culture medium with raw materials and water with the weight ratio ranging from 1:1.2 to 1:1.3, wherein the raw materials comprise, by weight, 45%-55% of paper pulp, 36%-44% of bagasse, 5.4%-6.6% of corn flour, 0.9%-1.1% of sugar, 0.9%-1.1% of gypsum, 0.9%-1.1% of lime and 0.9%-1.1% of calcium superphosphate; (2) cultivating stock cultures formed after tube culturing is carried out on bacterial strains, transplanting the stock cultures into the culture medium after hyphae are fully grown; (3) conducting liquidation to sample cultures, namely stock cultures, generated in the step (2), adding sterile water to conduct dilution according to the requirement that the weight ratio of spawn blocks to the sterile water ranges from 1:100 to 1:500, and evenly stirring the mixture.

The invention discloses an elastic microfluidic chip for tuberculosis detection. The elastic microfluidic chip for tuberculosis detection comprises a lower base plate, a PDMS elastic reaction cavity and a top-layer chip module, wherein the top-layer chip module comprises a filter layer, a lower-layer double faced adhesive tape, a mixed layer, an upper-layer double faced adhesive tape, an upper closure piece and a rubber stopper. Plasma separation of a whole blood sample to be tested, simultaneous mixing of plasma and a sample diluent and volumetric quantification and detection of the diluted sample are executed. In the top-layer chip module, the diluted sample is driven to lateral flow fluorescent paper strip in a chip detection area to complete the immunochromatographic reaction, and singles of a test line and a control line on the fluorescent paper strip corresponding to the sample are read through simultaneous immunochromatographic reaction on a plurality of sample culture solutions, and therefore negative and positive judgement of a tuberculosis detecting result can be achieved. The elastic microfluidic chip has the advantages of small volume, simple structure, convenient operation and low cost, and lays a good foundation for achieving rapid on-site tuberculosis detection.

The invention relates to an organic arsenic feed additive discharge pre-alarming monitoring method in a water environment system, belonging to the environmental monitoring technical field, comprising the following steps: preparing tetrahymena culture medium; preparing arsanilic acid standard sample solution/roxarsone standard sample solution; collecting the actual water sample and separating and extracting the organic arsenic feed additive compound; inoculating the tetrahymena into the prepared culture medium and then subpackaged the inoculated culture medium into the sample culture tubes; respectively adding the organic arsenic feed additive standard solution into the sample culture tubes and respectively measuring the corresponding tetrahymena cell density using absorptometry; making the actual water sample intervening the corresponding tetrahymena biological model and analyzing and determining the corresponding tetrahymena cell density; based on the comparison of the tetrahymena cell density and the contrast of the corresponding tetrahymena cell density after the actual water sample intervention, to obtain the concentration range of the pollutant presence, thereby pre-alarming the discharge condition of the organic arsenic feed additive in the corresponding actual water environment.

The invention discloses an experimental device and an experimental method for simulating lake wave disturbance, belonging to the field of water environment experiments. Aiming at the problems in the prior art that the environment of the experimental device is not uniform, the assembly is complicated, and the operation is tedious, the present invention provides an experimental device and an experimental method for simulating wave disturbance in a lake. Including the base of the test bench, the periphery of the base of the test bench is provided with a support, the support is arranged around and above the base of the test bench, the base of the test bench is provided with a sample culture pool, the disturbance device is arranged on the support, and extends into the sample culture pool through the support. The motor rotates in a circular motion to make the periodic motion of the up and down straight line of the pole. Through the action of the frequency regulator on the motor, the speed of the motor is the same, so that the left and right poles move in different straight lines, and the mechanical energy is converted into potential energy. Simulated The lake waves are irregular three-dimensional waves, which can realize realistic simulation environment, simple device and convenient operation.

The invention discloses a method of detecting influence on early apoptosis of cells due to total particulate matters in cigarette smoke. The method includes the steps of: 1) pre-treating to-be-detected substances; 2) preparing a human lung fibroblast single cell suspension liquid; 3) calculating cell concentration of the human lung fibroblast single cell suspension liquid; 4) performing cell inoculation to the human lung fibroblast single cell suspension liquid; 5) grouping the to-be-detected samples; 6) setting detection dose of the to-be-detected samples; 7) preparing a to-be-detected sample culture substance; 8) incubating the to-be-detected samples; 9) collecting incubated products of the to-be-detected samples; and 10) calculating influence ratio of early apoptosis of cells. Through optimization of sample detection dose and correct selection on target cells, the method can accurately and effectively detect the influence on early apoptosis of cells due to the total particulate matters in cigarette smoke. As a complementary method for cytotoxicity detection for the total particulate matters in cigarette smoke, the method distinguishes the influence between necrocytosis and cell apoptosis of the product.

A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

The invention discloses a method for detecting the influence of an electronic cigarette product on cell active oxygen secretion. The method includes the following steps of pretreatment of to-be-detected samples, preparation of a human lung fibroblast unicellular suspension liquid, calculation of the cell concentration of the human lung fibroblast unicellular suspension liquid, cell inoculation of the human lung fibroblast unicellular suspension liquid, to-be-detected sample grouping, to-be-detected sample detection dose setting, preparation of to-be-detected sample culture products, incubation of the to-be-detected samples, adding of a positive control, probe loading, sample measurement and active oxygen level calculation. Through the sample effective treatment, the detection dose optimized setting and the target cell correct selection, the method can effectively and accurately detect the influence of the electronic cigarette product on the cell active oxygen level.

The invention relates to a device for carrying out bacterial culture on a blood sample and particularly relates to a blood sample culture device. The blood sample culture device comprises a magnetic stirring device, wherein the a blood culture flask is fixed on the magnetic stirring device, a liquid medium is put in the blood culture flask, and a real-time detection and sampling device is fixed on the blood culture flask. The blood sample culture device is characterized in that the real-time detection and sampling device is provided with two channels, wherein the first channel is connected with a sampling pipe, a second channel is sequentially provided with a CO2 sensor and a pressure sensor, and the CO2 sensor and the pressure sensor are communicated with the inside of the blood culture flask through a hollow channel in the real-time detection and sampling device and are also linked with a computer. The blood sample culture device has the advantages of short detection cycle and high sensitivity, integrates multiple detection techniques and makes up the defects caused by single use of a pressure monitoring method.

The invention relates to biological sample culture detection equipment and in particular relates to a blood culture detection positive reporting device. The blood culture detection positive reportingdevice comprises a culture bottle, a connector, a carbon dioxide sensor and a computer, wherein a culture medium is filled into the culture bottle and a blood sample is collected; a butyl rubber stopper is arranged on an opening of the culture bottle and is used for sealing the opening. The culture bottle is simple in structure and low in production cost; the connector has a hollow design and a puncture needle is arranged at the lower end of the connector and is used for puncturing the butyl rubber stopper at the opening of the culture bottle, so that a cavity of the culture bottle is communicated with the carbon dioxide sensor through the connector; a filtering membrane is arranged in a pipeline of the connector; an oxygen consumption bag can be additionally arranged to culture an anaerobic sample; the bag is internally provided with an oxygen consumption agent so that the growth of anaerobic bacteria is facilitated. The carbon dioxide sensor is communicated with the connector and isused for sensing a concentration change state of carbon dioxide in the culture bottle and judging a growth state of microorganisms in a culture solution; meanwhile, the carbon dioxide sensor is connected with the computer and a positive culture bottle is reported to an operator in a laboratory.

A culture monitoring system includes: an image acquisition device that is arranged in an incubator and acquires an image of a biological sample cultured in the incubator; and a controller that controls the image acquisition device. The controller includes an estimator that estimates a state of a temperature of the biological sample with respect to a set temperature of the incubator, and a device controller that restricts an operation of the image acquisition device at least according to the state.

The invention discloses an experimental method for researching the response of mulching film degradation characteristics to moisture conditions. The method comprises the following steps: (1) sample culture: burying a sample mulching film into soil of each experimental group with different moisture contents; (2) sample collection; (3) sample determination: determining the degradation rate of the mulching film, the surface structure of the mulching film, the chemical structure of the mulching film, soil microbial biomass carbon, nitrogen, soil enzyme activity and the like; and (4) result analysis. According to the invention, a landfill culture test of the mulching film is carried out; the degradation time and the degradation degree of different PBAT biodegradable mulching films are analyzed;the degradation characteristics of the PBAT biodegradable mulching film under different soil moisture conditions are researched by combining scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) analysis, the influence of the PBAT biodegradable mulching film on soil microorganisms and enzyme activity is discussed, a mulching film degradation evaluation method is established, and a theoretical basis is provided for the popularization and application of the biodegradable mulching film.

The invention discloses a screening method of nutritive salt for pollutant enhanced bioremediation. The screening method comprises the steps of 1) adding a culture medium into liquid samples containing pollutants, then, observing the growing situation of floras, and carrying out baseline screening; 2) adding different varieties of nutritive salt of different proportions into the liquid samples containing the pollutants, carrying out shake-flask culture, and testing the amount of microorganism colonies through plate count and an ATP bioluminescence detector; and 3) detecting concentration of the pollutants in the pollutant-containing liquid samples cultured through adding of nutritive salt, evaluating the pollutant enhanced bioremediation effect of nutritive salt according to a result, and acquiring an optimal nutritive salt formula.

The invention relates to a precise lung cancer tissue location method which is based on an SVM model and combines with an image. The method comprises the following steps: (1) culturing two kinds of cell lines; (2) placing the two cultured cell lines on slides, and measuring Raman spectra of cells by adopting a confocal Raman spectrometer; (3) preprocessing the measured Raman spectra of the cells;(4) performing characteristic extraction on the preprocessed Raman spectra of the cells, wherein the extracted characteristics are characteristic peak positions and intensity ratios of the characteristic peak positions; (5) applying correlation analysis among multiple variables to the extracted characteristics; (6) for the characteristics extracted in the steps (4) and (5), performing classification recognition on spectral data by combining an SVM classifier; and (7) selecting a residual sample and testing, thus obtaining precision, sensitivity and specificity of the cells, and for a sample classified mistakenly, further distinguishing by utilizing a dyed image or Raman imaging. The method provided by the invention can eliminate the phenomenon that recognition rate is relatively low due toan error in an experiment or a sample culturing process.

The invention relates to a pseudomonas strain and identification and application methods thereof in the technical field of microorganisms. The rhodococcus strain has a 16S rDNA (16S ribosome deoxyribonucleic acid) gene sequence shown as SEQ ID NO.1, a strain name of SINOPEC21 and a preservation number of CCTCC NO: M2016111 in China Center for Type Culture Collection. The abundance of the pseudomonas strain in soil above an oil reservoir is positively related to the concentration of floating gaseous hydrocarbons of the oil reservoir, so that the pseudomonas strain can serve as an oil exploitation microorganism to mark areas with high hydrocarbon leakage above the oil reservoir; meanwhile, the pseudomonas strain is high in abundance in soil above the oil reservoir and saves amplification culture, the 16S rDNA conserved sequence of the pseudomonas strain SINOPEC21 in soil through improved primers, so that the concentration of floating gaseous hydrocarbons of the oil reservoir can be determined accurately and efficiently, and the deficiency of long detecting cycle and high requirements on purity of sample culture in traditional physiological and biochemical detection can be solved.

The invention relates to the technical field of tissue engineering, and in particular relates to a detection method of a promoted cell proliferation rate of freeze-dried powder. The method can be used for accurately detecting the promoted cell proliferation rate of freeze-dried powder. The embodiments of the invention provide a detection method of the promoted cell proliferation rate of freeze-dried powder. The detection method comprises the following steps: 1) dissolving to-be-detected freeze-dried powder in a complete culture medium to obtain a sample culture medium which has a first osmotic pressure; 2) by taking the complete culture medium without the to-be-detected freeze-dried powder as a control medium, adjusting the osmotic pressure of the control medium to be equal to the first osmotic pressure; 3) carrying out culture on the same number of cells under the same condition by using the obtained sample culture medium and the control medium respectively, and obtaining sample cells and control cells after a first preset time; and 4) carrying out MTT (methylthiazolyldiphenyl-tetrazolium bromide) dyeing and light absorption value detection on the obtained sample cells and control cells, and comparing the light absorption values of the sample cells and the control cells to obtain the promoted cell proliferation rate of a sample in respect to a control.

Disclosed in the invention is a full-automatic electrically-controlled microscope sample bench. The bench is characterized in that the bench comprises a plurality of microscopes, an electrically-controlled translation stand, a sample stand, a driving device, a switching power supply, and a controlling host. The plurality of microscopes are uniformly distributed at the same horizontal line. The sample stand is arranged at objective tables of the microscopes and includes a plurality of grooves for placing sample culture dishes; and one end of the sample stand is connected with the electrically-controlled translation stand. The electrically-controlled translation stand includes a sliding rail and a sliding plate arranged at the sliding rail; and the sliding plate is connected with the sample stand and can drive the sample stand to move. The driving device is connected with the switching power supply and is powered by the switching power supply; and the driving device is also connected with the controlling host by a data acquisition card; and the data acquisition card is used for converting a control signal from the controlling host into an electric pulse signal and sending the converted signal to the driving device. And control software is installed inside the controlling host and is used for controlling the motion of the electrically-controlled translation stand and collecting microscope information.

The invention discloses a device and method for screening and separating circulating tumor cells and application. The device comprises a blood sample culture chamber and a cell screening chamber whichare arranged from top to bottom; the blood sample culture chamber and the cell screening chamber are fixedly connected, and the cell screening chamber communicates with the blood sample culture chamber; the CTCs and blood cells in a blood sample are separated in the blood sample culture chamber by means of attraction action of a magnet; and biological filtering membranes are arranged in the cellscreening chamber, and the biological filtering membranes are used for intercepting the CTCs and filtering a nanometer material or the blood cells. By arranging the blood sample culture chamber, the CTCs and the blood cells can be separated in the blood sample culture chamber due to the fact that the magnetic nanometer material is attracted by the magnet, and CTCs loss caused by traditional CTCs separation by means of size difference is avoided, so that the detection accuracy is improved; and by arranging the cell screening chamber, the CTCs are filtered by the biological filtering membranes,so that fast screening and separation of the CTCs are achieved.

The invention discloses a detection method of the biological activity of vascular endothelial growth factors. The method comprises the following steps of (1) adding trypan blue into collected human umbilical vein endothelial cells; performing counting after the uniform mixing; regulating the cell concentration to (2 to 10)*10<4>mL according to the counting result; (2) culturing the regulated cells for 3 to 10h in a CO2 culture box; (3) performing concentration gradient dilution on the endothelial growth factors; adding all gradient dilution endothelial growth factor solutions into cells cultured in the step (2); after the uniform mixing, performing culture for 80 to 100h in the CO2 culture box; (4) adding Alma blue color developing liquid into a mixed solution of the vascular endothelial growth factors and the cells cultured in the step (3); after uniform mixing, performing culturing for 1 to 10h in the CO2 culture box; (5) performing fluorescence intensity detection on the sample cultured in the step (4). Through the activity detection on the vascular endothelial growth factors, the use quantity of the vascular endothelial growth factors in different batches is regulated, so that the activity detection result is stable and more accurate.

The present invention relates to the field of culturing of biological samples and in one form provides feedback in a biological sample culturing system to improve the viability of biological samples wherein the feedback comprises one or a combination of: measuring and manipulating environmental parameters operatively associated with the biological sample culturing system, and; measuring and manipulating culturing media parameters operatively associated with the biological sample culturing system. In another form the invention provides control of the culturing of biological samples in a biological sample culturing system comprising the steps of: mixing individual culture media components in response to one of a predetermined user selection or a predetermined user profile; dispensing the mixed media components into a preselected culturing pod containing at least one biological sample; providing feedback for the mixing step by measuring one or a combination of environmental parameters and culturing media parameters of the at least one biological sample.

The invention discloses an angle-deflected biological sample culture and observation integrated device. The device comprises a base body, a top cover and a temperature controller, wherein a base is arranged under the base body, a hydraulic rod is embedded and connected in the base, the top end of the hydraulic rod is connected with a roller, the inner side of the base is provided with a culture chamber, the lower portion of the right side of an adjusting plate is provided with a carrying plate welded to the inner side of the base body, the bottom of the adjusting plate is connected with a supporting plate, the bottom end of the supporting plate is connected with a pull plate, the outer side of a latch is connected with a connecting rod, the top end of a pull rod is provided with a handle,and the outer side of the top of the pull rod is provided with a spring. The angle-deflected biological sample culture and observation integrated device has the advantages of facilitating the angle defection, the rapid observation and use, the separate cultivation of samples, the regulation of the samples, the guarantee of sample display and the stable adjustment of growth environment and other conditions.