43 results about "Sample culture" patented technology

Embodiments of the present disclosure provide a biological sample imaging device, including an incubator with an observation window provided on one side; a biological sample cultivation module provided inside the incubator; an imaging module provided outside the incubator for collecting images of a biological sample cultured in the biological sample cultivation module through the observation window, so that the growth process can be dynamically captured, and the environment in the incubator can be conveniently controlled to adapt to various scientific research scenarios. In addition, the isolation inside and outside the incubator can reduce the interference of the imaging module on the biological sample.

The invention discloses a biomedicine standardized cubesat platform and a working method, and relates to the technical field of space.The biomedicine standardized cubesat platform adopts a 3U cubesat framework, the top layer of the 3U cubesat framework is provided with a satellite bus module, the middle layer of the 3U cubesat framework is provided with an energy and environmental control life support module, and the bottom layer of the 3U cubesat framework is provided with a biological experiment module; the satellite bus module is in bidirectional communication with the energy and environment control life support module and the biological experiment module through data interfaces. The satellite bus module further communicates with a ground station; the energy and environment control and life support module supplies energy to the satellite bus module and the biological experiment module through electrical interfaces, and provides oxygen supply, water supply and environment regulation and control support for the biological experiment module through a fluid transmission channel. Through a standardized and modularized cubesat structure, the functions of biological sample culture, environment regulation and control, real-time monitoring, data return and the like are integrated, and a low-cost and quick-response technical support is provided for space life science experiments.

The invention discloses a composition and a method for constructing ovarian organoid based on a clinical tissue sample. The composition comprises a sample preservation solution, a sample digestive juice and a sample culture medium, the method comprises the following operation steps: transporting a preservation solution A to a laboratory, and pretreating to obtain tissue fragment precipitates; carrying out resuspension incubation on the tissue fragment precipitate by using digestive juice B for digestion; collecting tissue blocks which are not completely digested and filtered, and performing secondary digestion to obtain single cells; combining the cells obtained in two times to obtain the required single cell; adding matrigel, then adding the mixture into a pore plate, and putting the pore plate into an incubator until the matrigel is solidified; and taking out the pore plate, adding a culture medium L2, and culturing to obtain the ovarian organ constructed based on the clinical sample. According to the method, the ovarian organoid is efficiently constructed from the ovarian tissue sample; the provided culture solution formula makes up the dilemma that normal ovarian tissue organoids are difficult to form and normal tissue-derived organoids are difficult to passage at present, and reduces the burden and experiment cost brought by sampling in the disease research process.

The invention discloses an on-line bacterial colony counting device for a disinfection tank. The on-line bacterial colony counting device comprises a counting mechanism, a cover body shell, a control operation table and a culture dish storage mechanism, the cover shell is arranged on the front side of the top of the counting mechanism. The two culture dish storage mechanisms are arranged at the rear ends of the left side and the right side of the outer portion of the counting mechanism correspondingly. The on-line bacterial colony counting device for the disinfection tank realizes full-process automation from sample culture, specified sample taking, transferring and positioning to double-sided counting, greatly reduces the manual intervention intensity, avoids pollution and subjective errors caused by manual operation, improves the counting working efficiency, is suitable for continuous treatment of batch samples, and improves the working efficiency. And through the design that angle adjustment is matched with double-sided synchronous counting and sample rotation covers the whole area, impurity interference is effectively eliminated, counting dead angles are eliminated, and the comprehensiveness and accuracy of a counting result are improved.

The utility model discloses a culture bottle for indoor soil culture, and relates to the technical field of soil sample culture. A 150 cylindrical brown glass wide-mouth bottle is selected as the culture bottle, the brown glass can prevent light pollution, the culture effect and quality can be further guaranteed, the cylindrical culture bottle can be arranged in the culture box in the maximum volume mode, the bottom of each silicone tube extends into the bottom of the culture bottle, and therefore the culture effect and quality are improved. Through the arrangement, gas in the culture bottle can be fully exchanged; by arranging the three-way valve, the installation stability of the silica gel tube can be guaranteed, nitrogen blowing can be carried out on the interior of the silica gel tube to guarantee the CO2 background value of a culture bottle, repeated uncovering is not needed, sample pollution is avoided, and continuous sampling measurement of a soil sample culture experiment is facilitated; the purpose of rapid sampling can be achieved through the injector, and the cultivation effect and quality are guaranteed.

The present invention is directed to a method for identifying i) metastatic potential of a cancer cell sample from a non-metastatic cancer or ii) potential for malignant alterations in a healthy cell, the method comprising the steps of: a) culturing a cancer cell sample from a non- metastatic cancer or a healthy cell sample in a composition comprising cancer-associated fibroblasts, CAFs, preferably in two-dimensional or three-dimensional cell culturing conditions; and b) monitoring morphological alterations in the sample cells during and / or after the culturing step a), and / or determining the expression level of biomarkers relating to metastasis in the sample cells during and / or after the culturing step a); wherein the sample cells which show morphological alterations relating to metastasis and / or changes in the expression levels of said biomarkers in comparison to the levels of said biomarkers measured from corresponding samples cultured in a composition comprising normal fibroblasts, NFs, are identified as having metastatic potential or potential for malignant alterations. The present invention is also directed to a system for culturing eukaryotic cells comprising a solid surface comprising at least one partition suitable for culturing eukaryotic cells, wherein said at least one partition contains a combined cell culture comprising i) cancer cells from a non- metastatic cancer or healthy cells and ii) cancer-associated fibroblasts, CAFs, wherein said partition is a well or chamber.

The invention relates to the field of biological sample culture, and particularly discloses a biological tissue sample preservation box, which comprises a storage box, which comprises box walls and a cavity defined by the box walls; the supporting piece is fixedly arranged in the cavity to support a test tube in which a specimen is stored; the extracting device is used for placing the test tube on the supporting piece and taking the test tube from the supporting piece; wherein the box wall forms an opening entering the internal space of the cavity; a sealing cover is fixedly arranged on the storage box to completely cover the cavity; a plurality of positioning grooves corresponding to the test tubes are formed in the supporting piece; the extraction device comprises an extraction pipe and a conveying belt; the extraction tube is provided with a transmission channel for guiding the test tube to get close to / away from the supporting piece along a first direction formed by the transmission channel; the conveying belt is arranged in the conveying channel and used for providing power for moving the test tubes; the biological tissue sample preservation box has the beneficial effect that the preservation environment cannot be affected during extraction.

The invention discloses a dual-channel sample transfer device, a full-automatic incubator and a management system. The dual-channel sample transfer device comprises a first transfer channel mechanism, a second transfer channel mechanism and a transfer part, the first transfer channel mechanism is arranged at a first position in the movement range of the transfer part and is used for leading in or leading out a sample in a first mode; the second transfer channel mechanism is arranged at a second position in the movement range of the transfer part and is used for leading in or leading out the sample in a second mode; the transfer part is used for taking the sample from the position for culturing the sample and transferring the sample to the first transfer channel mechanism or the second transfer channel mechanism; and the sample transfer mechanism is also used for taking the sample guided by the first transfer channel mechanism or the second transfer channel mechanism and transferring the sample to a sample culture position. According to the invention, a dual-channel sample transfer framework is realized through the first transfer channel mechanism and the second transfer channel mechanism, and dual-channel parallel sample transfer operation of biological samples is realized through cooperative cooperation with the transfer part.

Systems and methods are provided for automated incubation of large numbers of tumor organoids or other volumetric biological samples cultured from cells or other tissues. Label-free, non-destructive imaging of the samples is performed quickly and at high temporal resolution (e.g., once per day or more), allowing interventions (like the addition or subtraction of substances like water, glucose, buffers, or pharmaceuticals) to be applied to each sample based on the images, facilitating closed-loop per-sample control of sample growth. This can lead to improved incubation outcomes, like increased rates of generation of live samples and control of sample size or other sample properties. Image and other incubation data obtained via such automated incubation processes can be used to train machine learning models to plan interventions for the samples, further increasing incubation success rates and allowing for the incubation of rare and / or novel samples, for which reliable incubation protocols do not yet exist.

The invention relates to the field of microbiological detection, and discloses a sample culture-free efficient identification kit and a detection process, the sample culture-free efficient identification kit comprises a staining reagent, a scattering calibration and inhibition reagent, a quality control reagent and consumables, the staining reagent is a multi-target fluorescent chromosome system, and the scattering calibration and inhibition reagent is a scattering calibration and inhibition reagent. The scattering calibration and inhibition reagent is used for double-scattering signal calibration and scattering noise inhibition, the quality control reagent is used for validity verification of a detection result, and the consumable is used for adaptive imaging detection. Through cooperative adaptation of a staining reagent, a scattering calibration and inhibition reagent, a quality control reagent and consumables, microorganism multi-target specific marking can be realized by means of a multi-target fluorescent chromosome system without depending on microorganism culture, and scattering signal interference is eliminated by combining the scattering calibration and inhibition reagent so as to guarantee the accuracy of detection signals. The validity of a detection result is verified through a quality control reagent, so that rapid identification and characterization of microorganisms in a complex sample matrix are realized.

The utility model discloses a microbiological detection device, relates to microbiological technical field, including bottom plate, the upper side surface middle part of bottom plate is fixedly connected with the lower end of round bar through bearing, the upper end of round bar is fixedly connected with fixed mount, the outer side surface of fixed mount is fixedly connected with the inner side surface of rotating ring, and the rotating ring is fixedly connected with the outer side surface of rotating ring. Fixing grooves are formed in the upper side surface of the rotating ring at equal angles, a push plate is arranged in each fixing groove, the side surface of each push plate is slidably connected with the inner side surface of the corresponding fixing groove, the lower side surface of each push plate is fixedly connected with a push rod, and each push rod is slidably connected with a sliding hole formed in the bottom face of the corresponding fixing groove. The lower end of each push plate penetrates through the corresponding sliding hole and extends to the lower side of the rotating ring, the upper side surface of the bottom plate is fixedly connected with a convex block, the convex block is in contact with the lower end of the push rod, a plurality of sample culture dishes are uniformly mounted, samples are automatically switched, and the culture dishes are convenient to take and place.

The application discloses a constant-temperature incubator and a temperature control system thereof, and relates to the field of incubators. The constant-temperature incubator comprises an incubator body and a temperature control box, a plurality of independent culture cavities are formed in the incubator body, air inlets and air outlets are formed on the two sides of the culture cavities respectively, the temperature control box is fixedly installed on the side surface of the incubator body, a main air duct, a plurality of branch air ducts and a plurality of return air ducts are installed in the temperature control box, and an air inlet cavity in communication with the main air duct and the outside is formed in the side wall of the temperature control box. The cooperation of the main air duct, the branch air duct and the return air duct in the temperature control box constructs a complete air circulation system, and temperature loss is reduced. Meanwhile, a single fan can be controlled to open and close different branch air ducts in cooperation with an air valve actuator, the number of culture cavities to be started is adjusted, all air duct components need not be started, and the adaptability of the equipment to different sample culture numbers is improved.

The invention belongs to the technical field of soil improvement, and particularly relates to a microorganism and organism synergistic efficient soil improvement method, which comprises the following steps of: separating strains from soil to be improved, independently culturing, then carrying out mixed culture, matching the independently cultured strains or the mixed strains subjected to mixed culture with wormcast according to requirements, and regulating and controlling the ratio, so as to obtain the improved soil. According to the method, on the basis of an informationized experiment database and an intelligent strain sample culture system, a quick response chain from experiment to task development to production is established, soil improvement is efficiently and specifically implemented, in the improvement process, the type of the wormcast organic fertilizer is selected according to the soil improvement condition, and the improvement efficiency is improved. The improvement effect is regulated and controlled through hierarchical addition, and the improvement method is simple, convenient and easy to implement.

The invention provides an X-ray plate microscopic imaging loading device for a living embryo sample and a long-term microscopic imaging method, and belongs to the technical field of X-ray plate microscopic imaging. The loading device mainly comprises an FEP cabin body, a fixing ring, a sealing gasket, a sealing cover, a liquid adding capillary tube, a liquid pumping capillary tube, a gas supply capillary tube and a pressure balance capillary tube. The FEP cabin body is prepared through a forming device. The loading device disclosed by the invention can realize rapid and lossless loading of small samples such as embryo-like samples; the closed design can provide a sterile environment; the liquid adding / pumping capillary tube is matched with the injection pump, and the gas supply / pressure balance capillary tube is matched with the gas controller, so that the replacement of a culture solution and the maintenance of a gas environment required by the living embryo sample culture can be automatically realized, and the normal growth and development of the sample in the X-ray plate microimaging process are ensured. The loading device can be matched with different light-sheet microscopic imaging systems, so that uninterrupted high-resolution microscopic imaging observation of the light-sheet microscope in the living embryo sample development process is realized.

The legionella culture device comprises a sealed culture box and a box cover, the upper portion of the sealed culture box is in sealed connection with the box cover through a sealing ring, a liquid adding opening is formed in the top of the box cover, and the liquid adding opening is in sealed connection with the box cover through a sealing plug; a plurality of GVPC culture medium plates and a gas production bottle are respectively arranged in the sealed culture box, the gas production bottle is a bottle with an opening at the upper part, and the opening at the upper part of the gas production bottle is arranged corresponding to the position of the liquid adding opening. The method comprises the following steps: collecting water sample liquid by using a special sampling device, filtering, concentrating and inoculating the water sample liquid into a GVPC culture medium plate, putting the GVPC culture medium plate into a sealed culture box, and reacting reaction liquid and reaction powder in the culture box to automatically generate carbon dioxide and water; on one hand, the conditions that carbon dioxide is difficult to obtain and the concentration of the carbon dioxide is unstable in the experiment process are avoided, on the other hand, the generated water ensures the humidity of the culture environment, the sample culture process is simplified, and the laboratory detection process is easier to realize.

This application discloses a circulating carbon dioxide incubator, belonging to the field of biological culture equipment technology. It includes an incubator body, an exhaust pipe, and an intake pipe. A limiting seat is fixed inside the incubator body, and a placement plate slides within the limiting seat. An exhaust box is fixed through the front end of the incubator body, and a first vent hole is opened on the exhaust box in the front area of ​​the lower placement plate. This circulating carbon dioxide incubator, by changing the traditional bottom humidification method, sets up humidification boxes inside the exhaust box and the conversion box. The positions of the humidification boxes correspond to the areas of different placement plates. A fan drives airflow circulation, allowing the moisture generated by the humidification boxes to be evenly distributed to all levels of the incubator. This solves the problem of uneven humidity distribution between different levels within the incubator, reduces dehydration of upper-layer samples and mold growth in lower-layer samples due to excessive humidity, and improves the biological sample culture effect.

The invention relates to the technical field of food safety, and discloses a portable food surface microorganism sampling culture device which comprises an outer box, a box cover is movably mounted at the top of the outer box, and a material containing assembly which extends to the inner side of the outer box and is connected with the outer box is arranged on the right side of the outer box; a moving assembly is arranged between the left side and the right side of the inner wall of the outer box, a handle is fixedly installed at the top of the box cover, a strap fixedly connected with the right side of the outer box is fixedly installed on the left side of the outer box, a culture mechanism is arranged on the inner bottom wall of the outer box, and a mixing mechanism is arranged on the inner side of the culture mechanism; a mounting mechanism is arranged at the bottom of the moving assembly, and a sampling mechanism extending to the bottom of the mounting mechanism is movably mounted on the inner side of the mounting mechanism. According to the portable food surface microorganism sampling and culturing device, by arranging the culturing mechanism and the mixing mechanism, the purpose of arranging the mixing mechanism is achieved; the purpose that the sampling head can be replaced is achieved.

The application discloses a microscope living body culture environment control system and method, relates to the monitoring control technical field, can be applied to the microscopic observation demand in the field of cell biology and medicine, and mainly solves the problem of heating and airflow disturbance interference micro-imaging of the existing microscope living body sample culture device. Mainly includes embedded controller, monitoring component, temperature control component and gas control component; wherein, the embedded controller determines real-time temperature monitoring data and real-time concentration of target gas based on real-time temperature sensing signal and gas concentration sensing signal; calculates temperature deviation and gas concentration deviation; when the temperature deviation and / or gas concentration deviation does not satisfy the corresponding target parameter deviation condition, determines the target control component and the control signal of the target control component according to the deviation that does not satisfy the target parameter deviation condition; the control signal is sent to the target control component. The application is mainly used for controlling the environmental parameters of living cells under microscope observation.

The utility model relates to a sample culture and detection system, which comprises an incubation culture device, a detection device and a control device, and the fluorescence detection device is used for detecting the sample in the incubation culture device. The sample culture and detection system executes the sample culture and detection method; the sample culture detection method comprises the following steps: incubating a sample in the incubation culture device by the incubation culture device; the fluorescence detection device is used for detecting the sample. The sample culture and detection system is used for incubating and detecting a sample so as to improve the detection efficiency.

The application discloses an automatic material supplementing oscillation incubator, which comprises a box body, a first fixing frame, a probe, a culture bottle, an electrode sensor, a sealing flexible joint, a collection box, connecting lines and an oscillation mechanism; the first fixing frame and the collection box are fixed on the inner wall of the box body; one end of the probe is fixed on the first fixing frame through the sealing flexible joint; the probe is internally provided with the electrode sensor; one end of the electrode sensor penetrates through the sealing flexible joint and reaches into the solution in the culture bottle; the other end is connected with the probe; the electrode sensor is connected with the collection box through the probe and the connecting lines. The probe is fixed on the one side fixing frame of the first fixing chamber through the sealing flexible joint, signal interference caused by shaking of the probe and the lines is reduced, and the detection precision of the sample culture condition in the culture bottle is improved.

The utility model relates to the field of gene detection, in particular to a sample culture device applied to gene detection, which comprises a box body and a side door rotationally connected to one side of the box body, and further comprises a refrigerating device arranged in the box body, a plurality of sample boxes capable of being pulled out in a sliding manner are arranged in the box body, and a plurality of through holes are formed in two sides of each sample box along the width direction. The refrigerating device is positioned on the side of the sample box; the side door is used for sealing the box body to enable the interior to be in a sealed state, the refrigerating device is a relatively mature prior art and is used for refrigerating and keeping constant temperature, the plurality of sample boxes are used for placing samples of different batches or different testers, and when the sample boxes are stored in the box body, cold air emitted by the refrigerating device enters from the through holes in the two sides to enable the samples to be in a low-temperature constant state; when a certain sample needs to be taken out, the corresponding sample box is independently pulled out, the other sample boxes continue to be located in the box body at the moment, little cold air overflows, the temperature change is low, and the situation that the other samples are affected by the temperature change and damaged can be effectively avoided.

The utility model relates to a culture dish and a sample culture system, the culture dish comprises a ventilation assembly, an upper dish body assembly and a lower dish body assembly; the upper dish body assembly comprises a dish cover and a dish body and is detachably connected with the dish body through threads. The lower dish body assembly comprises a dish bottom and a transparent piece, an observation window is formed in the bottom wall of the dish bottom, the transparent piece covers the observation window, the dish bottom covers the second end of the dish body through a thread, and a containing space is defined by the upper dish body assembly and the lower dish body assembly; the ventilation assembly comprises a first pipe body and a second pipe body which are communicated with the containing space. The air pressure and the gas composition in the culture dish are controllable, and the change process of a sample in the culture dish can be observed and recorded in real time. The culture dish is small in size, gas types and gas pressure in the culture dish can be accurately controlled by using a small amount of gas, gas consumption is reduced, and cost is reduced.

The utility model discloses a kind of rotating automatic sequencing devices of microbial sample, it is related to microbial sample detection technical field, comprising: conveying component, sequencing component and sample culture dish, the sequencing component is installed on conveying component, the sample culture dish on conveying component is guided by sequencing component, so that it is neatly sorted;Pushing component and rotating stacking component, the pushing component is installed on conveying component, the sample culture dish neatly sorted on conveying component is pushed to rotating stacking component by pushing component, so that it is vertically stacked one by one. By placing sample culture dish on conveying component, and guiding it by sequencing component, so that it is neatly sorted, subsequently by pushing component, the sample culture dish neatly sorted on conveying component is pushed to rotating stacking component one by one and is stacked, so that it is vertically stacked one by one, realize automatic sorting and arranging of sample culture dish, reduce manual operation, improve efficiency.

Aspects and embodiments relate to a biological sample culture unit, a biological sample culture module comprising at least two biological sample culture units and culturing apparatus including a biological sample culture unit or biological sample culture module. Aspects and embodiments also provide methods to culture biological samples within a biological culture unit, biological culture module or biological culture apparatus. All aspects utilise a biological sample culture unit comprising a first chamber configured to accommodate a biological sample, culture medium and a gas reservoir; a second chamber configured to accommodate a reservoir of culture medium and a further gas reservoir, the second chamber comprising a gas port configured to couple the further gas reservoir to a gas source; an inlet conduit linking the second chamber with the first chamber configured to allow flow of fluid between the second chamber and the first chamber in dependence upon a pressure difference between the gas reservoirs in the second chamber and the first chamber. Aspects support provision of a small footprint biological sample culture environment which is highly scalable, which can sustain a culture environment with controllable conditions for an extended period and in which maintenance of, and testing a biological sample within, the culture environment can be automated, thus facilitating high throughput, minimal disruption to biological samples under study and improved reproducibility.

The invention discloses an auxiliary experimental device for pharmacology, which relates to the technical field of experimental equipment for pharmacology, and is characterized by comprising a sample culture module, a multi-parameter sensor, a data acquisition and analysis module, an environment control module and a man-machine interaction module. The sample culture module provides a suitable environment for a sample, the multi-parameter sensor module integrates multiple sensors to monitor drug action parameters in real time, the data acquisition and analysis module processes and analyzes data, the environment control module maintains a stable environment, and the man-machine interaction module realizes convenient operation and data management. The device can comprehensively monitor the drug action process in real time, the richness and accuracy of experimental data are improved, human intervention is reduced, the experimental efficiency and reliability are improved, and powerful support is provided for pharmacological research.

The utility model relates to a sample culture device which comprises a base, a placing plate, a liquid separation part and a connecting part, wherein the placing plate is arranged on the base and is used for placing a culture dish; the liquid separation part is arranged above the placing plate; the connecting part is used for fixedly connecting the base with the liquid separation part; the liquid separation part comprises a plate body, a plurality of taper holes, a plurality of rubber plugs, a plurality of push rods and a pressing part; the taper holes are formed in the plate body and penetrate through the plate body; the rubber plugs are arranged in the taper holes in a one-to-one correspondence manner; the push rods are arranged on the lower sides of the rubber plugs in a one-to-one correspondence manner; the liquid separation cylinders are formed by protruding upwards from the upper side face of the plate body and annularly arranged around the taper holes in a one-to-one correspondence mode, and the liquid blocking frame is formed by protruding upwards from the upper side face of the plate body. The large end of the taper hole faces upwards, and each liquid separation cylinder is located in the liquid blocking frame. Due to the adoption of the technical scheme, time and labor are saved during use, and the workload of operators can be reduced.

The utility model discloses a cell culture pore plate box, which comprises an outer box and an inner box, a plurality of inner boxes are placed on the inner side of the outer box; the inner box is provided with a plurality of cavities; a cover plate is arranged on any inner box, and the multiple cavities are sealed through the cover plates; a separated pore plate box is designed, an outer box and a plurality of inner boxes are assembled and matched to form the structure of the pore plate box, and a cover plate is arranged on each inner box, so that sealing of a single inner box is realized, simultaneous culture of a plurality of samples is met, the samples can be independently taken out for subsequent detection according to actual culture conditions of different samples, the sample loading rate is high, and the pollution risk is reduced; the pore plate is high in utilization rate and saves resources.

The invention discloses a medical detection device, and relates to the field of medical detection, the medical detection device comprises a shell, a waste water tank is arranged on the bottom surface in the shell, and a first partition plate and a second partition plate are respectively arranged at two ends of the top surface of the waste water tank in the shell; a purified water tank and a culture solution tank are arranged on the two sides, located between the first partition plate and the second partition plate, of the top of the waste water tank correspondingly, first lead screws are rotationally connected to the two sides of the top face in the shell correspondingly, and linear motors are slidably mounted on the two sides of the top face in the shell correspondingly. The sampling, cleaning and culturing processes in the medical detection process are both set in the closed space, the dropper, the cleaning mechanism, the film feeding mechanism, the film removing mechanism and the film releasing mechanism are matched with one another, the whole culture process of a sample is completed, and the sample does not make contact with the outside before being cultured, so that the culture efficiency is improved. The influence of bacterial microorganisms in external air on the culture sample is reduced, so that the final detection result is more accurate.

The invention belongs to the technical field of soil acidity regulation and control, and discloses a neutral red soil acidification method based on multi-reagent regulation and control, which comprises the following steps: S1, soil pretreatment; s2, reagent selection; s3, setting a reagent application amount gradient; s4, sample culture; s5, index determination; s6, data analysis; the method has the following beneficial effects: 1, accurate regulation and control: by establishing a mathematical model, accurate regulation and control of soil pH can be realized, and excessive acidification or insufficient acidification is avoided; 2, the method is fast and stable, the citric acid can reach the target pH value in a short time, and the acidification effect is stable; 3, low salt accumulation: compared with some traditional methods (such as ferrous sulfate), the citric acid has lower salt accumulation while being effectively acidified; 4, operation is simple and convenient, specific adding proportion and operation steps are provided, and practical application and popularization are facilitated. And 5, environment friendliness: the citric acid has small influence on soil microorganisms and environment, and meets the requirements of green agriculture.

The utility model discloses a lightweight biological sample culture module device, which relates to the technical field of microbial culture and comprises a framework, a solution storage tank with two closed ends is arranged on the framework, and at least one separation piston in sliding seal fit with the tank wall of the solution storage tank is arranged in the solution storage tank. The interior of the solution storage tank is divided into at least two cavities for storing solutions by the separation piston; an axial through flow channel is formed in the separation piston, a one-way valve assembly is arranged in the flow channel, and a driving assembly for driving the one-way valve assembly to be opened is further arranged in the solution storage tank. The biological culture device is ingenious in structure, high in biological culture utilization rate and low in space occupancy rate, and astronauts can conveniently carry the biological culture device to go up and down due to the characteristics of light weight and small size of the biological culture device.