405 results about "Cultured cell" patented technology

The invention provides a gamma delta T cell preparation as well as a preparation method and application thereof, and belongs to the technical field of T cells. The liposome is prepared from the following raw materials in parts by weight: 15-20 parts of modified gamma delta T cell liposome, 1-3 parts of cell stimulating factors and 4-7 parts of culture medium freeze-dried powder, the modified gamma delta T cell lipidosome is prepared by embedding gamma delta T cells through lipidosome, coupling with acetylenic bond modified chitosan, and further mixing with MFAP4 protein and b4GALT1 protein. The culture medium freeze-dried powder is prepared by freeze-drying the culture medium in the engineering culture process of gamma delta T cells, and the cell stimulating factors are resveratrol and quercitrin. According to the gamma delta T cell preparation prepared by the invention, the survival ability and resistance of gamma delta T cells are improved, the anti-tumor activity of the gamma delta T cells is improved, and the gamma delta T cell preparation has relatively good antioxidant and anti-inflammatory effects, reduces the inhibition of inflammation on an immune system and has a very good treatment effect on pancreatic ductal adenocarcinoma.

This disclosure describes methods for improving umami and meaty flavors of cell-based food products. In particular, the disclosed method includes an amino acid integration method where cultured cells and exogenous amino acids (e.g., glutamic acid and aspartic acid) are combined and heated, resulting in a synergistic improvement of umami flavors. The disclosed method further includes a browning method by which a cell suspension is dehydrated and heated at high temperatures (e.g., greater than 140C) to induce non-enzymatic browning and caramelization reactions in the cell suspension. The cell-based flavoring product can be combined with a food product as a flavor, aroma, and color enhancer. The disclosed methods may use one or both of the amino acid integration method and the browning method to improve organoleptic properties of cell-based food products.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention relates to a multifunctional integrated organoid culture device. The device comprises a main body, and a liquid input module, a temperature control module and an organoid module which are fixed on the main body, the main body comprises an adjusting assembly and a main control chip which are connected with each other, and the main control chip is connected with the temperature control module and the liquid input module and used for adjusting the flow speed of the liquid input module and the temperature of the temperature control module; the liquid input module is connected with the organ-like culture module and used for conveying cell sap to the organ-like culture module, the organ-like module is provided with a cell anchoring array and used for fixing and culturing cells, and the temperature control module is located between the liquid input module and the organ-like module and used for controlling the temperature of the organ-like culture module. The temperature detection module is used for detecting the temperature of the liquid input module and adjusting the temperature of the organ-like module. Compared with the prior art, the invention has the advantages of high control precision, multi-cell fusion, abundant functions and the like.

The invention discloses a method for culturing NK (Natural Killer) cells. The method comprises the following steps: inoculating a mononuclear cell into a T25 cell culture flask, wherein the T25 cell culture flask is coated by a coating factor in advance; carrying out culture treatment on the inoculated mononuclear cells in a culture system containing an NK cell culture medium containing an activation amplification factor and plasma so as to obtain NK cells; wherein the coating factor, the activation amplification factor and the NK cell culture medium are derived from an NK cell amplification kit. According to the method provided by the embodiment of the invention, the dosage of the amplification reagent can be reduced, the amplification multiple of the NK cells is improved, and the production cost is reduced.

A method for in situ differentiation of stem cells within a cell culture bioreactor is provided. The method includes providing a bioreactor vessel having a cell culture chamber, the cell culture chamber having an inlet for introducing fluid into the cell culture chamber and an outlet for introducing fluid out of the cell culture chamber. The bioreactor vessel also includes a cell substrate disposed within the cell culture chamber for culturing cells on the cell substrate. The method includes seeding undifferentiated stem cells onto the cell substrate within the cell culture chamber and perfusing the cell culture chamber with a differentiation medium to promote differentiation of the undifferentiated stem cells into specific cell lineages, thereby causing the undifferentiated stem cells to become differentiated cells.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The invention relates to the technical field of biology, and discloses a construction method of a gastric cancer organoid culture system, which comprises the following steps: preparation of a conditioned medium: culturing L-WRN cells and collecting the conditioned medium; preparing a gastric cancer organoid culture medium, and mixing the conditioned culture medium, the basic culture medium, a plurality of growth factors and additives; and extraction and culture of organoid: separating cells from gastric cancer tissues, mixing the cells with matrigel, and culturing by using the prepared culture medium. According to the method, the repeatability and the stability of the organoid model are improved, the response consistency of the organoid in drug screening is improved, and the reliability of the model in disease mechanism research and personalized medical application is guaranteed.

Disclosed herein is a cell culture device (1) for culturing cells and / or organoids, the cell culture device comprising a cell containment unit (2) comprising at least one cell culture chamber (3) configured to contain a liquid cell culture medium (9) and a cell structure, wherein the cell culture chamber (3) comprises: a gas vent opening (4) configured to vent the cell culture chamber; a pressure chamber (5) comprising a pressure chamber inlet (6); a channel (7) connecting the at least one cell culture chamber (3) and the pressure chamber (5); the cell culture device further comprising a first pressure generator (8), in particular a pump, connected to the pressure chamber inlet and configured to temporarily pressurize the pressure chamber with a pressure pulse; wherein the at least one cell culture chamber (3), the pressure chamber (5) and the channel (7) are configured such that the pressure pulse from the first pressure generator (8) propagates from the pressure chamber (5) through the channel (7) to the at least one cell culture chamber (3) and in particular into the liquid cell culture medium (9).

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

1. The name of the design product: 3D human-like intelligent cell culture equipment. 2. The use of the design product: the equipment used for culturing cells. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

A method of coating a cell culture substrate in situ in a bioreactor is provided that includes providing a bioreactor vessel having a cell culture chamber with an inlet for flowing fluid into the cell culture chamber and an outlet for flowing fluid out of the cell culture chamber, and a cell substrate disposed in the cell culture chamber for culturing cells. The method includes providing a coating solution for coating the cell substrate; inputting the coating solution into the cell culture chamber such that the coating solution contacts the cell substrate; and removing an excess of the coating solution from the cell culture chamber. After removing the coating solution, a coated cell substrate remains in the cell culture chamber.

The invention discloses a multi-cavity cell bag, and relates to a cell culture bag, which is flatly placed on a platform, is used for culturing cell balls, and comprises a cell bag body, a plurality of sub-cavities for culturing the cell balls and a total cavity for collecting cultures and reducing the density of the cell balls are arranged in the cell bag body, the branch cavities are connected with the main cavity through channels with controllable on-off states, each branch cavity is provided with a first sample inlet and a first liquid outlet, the main cavity is provided with a second sample inlet, a sampling port and a second liquid outlet, and the branch cavities and the main cavity are respectively provided with a first air exchange port and a second air exchange port. According to the invention, high-density cell sphere induction is firstly carried out through the sub-cavities and then collected to the total cavity for low-density culture, so that the requirements of different growth stages of the cell spheres on the environment are met, the formation rate and the culture quality of the cell spheres are improved, and a high-quality experimental material is provided for related research of the cell spheres.

The present invention relates to a low-serum medium composition for culturing Vero cells, and a method for culturing Vero cells and a method for producing a virus, both using the same.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The utility model relates to the technical field of biomedicine, and discloses a toxicological cell incubator which comprises a working panel and supporting legs fixedly mounted on two sides of the lower end of the working panel, firstly, a movable door is taken down, and culture cells are placed on a culture bracket; after placement is completed, the movable door can be inserted into the closed incubator along the connecting ring again, the air tightness of the device can be improved under the action of the rubber mat, the concentration of toxic gas in the incubator needs to be adjusted during toxicological experiments, the toxic gas can be filled into the device through the gas filling device, and then the toxic gas can be filled into the incubator. When the test is completed and the device is taken out, because the device is filled with toxic gas, the flowing direction of the gas can be switched through the adjusting assembly, and the toxic gas is guided into the filtering assembly, so that the gas is filtered and purified, and the toxicological cell culture box is relatively good in air tightness when being used; after the test, toxic gas used in the test can be conveniently filtered and purified, and the overall safety of the device is improved.

The present invention provides mesh rolled scaffold devices and bioreactor systems that can provide a large surface-to-volume ratio for expanded cell culture. The mesh rolled scaffolds minimize shear stress on cultured cells and support sufficient and uniform mass transfer rates of gases and nutrients. The mesh rolled scaffolds can be connected to a media source via holders in bioreactor systems to support large-scale expansion and maintenance of cell cultures. The present invention also provides the bioreactor systems that can include dialyzers and heat exchangers to modify media and other fluids passing through the systems. The bioreactor systems include media and other fluid reservoirs that can support high stirring rates between about 100 and 10000 rpm, and the overall systems can be pressurized between about 1 and 10 atm to increase gas exchange rates.

An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

The application relates to the technical field of cell culture, in particular to a method for efficiently culturing NK cells in vitro and research on the antitumor activity of the NK cells in vitro. The method comprises the following steps: on day 0, resuspending PBMCs with a first-stage activation culture medium and culturing for 20-25 hours; on day 1, supplementing a second-stage activation culture medium and continuing to culture for 45-50 hours; on day 3 and day 5, counting the cells, respectively supplementing a third-stage culture medium, and culturing to day 7; on day 7, counting the cells, supplementing a fourth-stage culture medium, and culturing and standing; on day 9, day 11 and day 13, respectively supplementing the fourth-stage culture medium, and culturing to day 15 for harvesting. The method is simple in operation, low in cost, high in quantity of obtained NK cells, high in purity of the NK cells and high in killing activity of the NK cells.

The invention belongs to the technical field of in-vitro cultured calculus bovis, and provides in-vitro cultured calculus bovis and a preparation method thereof. The preparation method comprises the following steps: (1) separating cells; (2) primary culture; (3) cell inoculation and cultivation: adding the serum-free culture medium into the carrier matrix, putting the carrier matrix into a reactor, and performing cell inoculation on the carrier matrix after passage; the inoculated culture comprises a proliferation period; a synthesis period; a crystallization period; and (4) collecting and purifying the calculus bovis, namely stopping culturing after 45 days, and sequentially collecting, cleaning, degreasing, drying and crushing the calculus bovis. The preparation method aims at solving the problems of long culture period, unstable content of effective components, high production cost, large batch difference and the like in the existing in-vitro calculus bovis culture technology by optimizing a culture medium formula, regulating and controlling a culture process and a purification process.

The invention belongs to the field of biological medicine, and relates to a curcumin-loaded bone marrow mesenchymal stem cell source exosome which is obtained by the following steps: step 1, culturing BMSCs (bone marrow mesenchymal stem cells): simultaneously adding 10% of fetal calf serum and 1% of myclostreptomycin into an MEM culture medium, culturing at 37 DEG C under 5% CO2, and counting and subculturing when the cell density reaches 90%; 2, dissolving Cur in a DMSO solution, preparing a mother solution with the culture medium, and filtering and diluting to 30-50 [mu] g / mL; adding into BMSCs (bone marrow mesenchymal stem cells) with the cell density of 85-100%, and culturing for 12-36 hours; collecting supernate, and carrying out gradient centrifugation to obtain the mesenchymal stem cell source exosome loaded with curcumin. The invention provides a novel administration route which depends on the self function of the mesenchymal stem cell source exosome and the self function of the curcumin at the same time.

The objective is to provide a novel method for improving the efficiency of osteogenic differentiation induction based on immobilized BMP-2. [Solution] A method for stimulating cells with ligand molecules, comprising a stimulation step of applying physical stimulation to the cells and / or ligand molecules, wherein the ligand molecule is bound to or interacts with a receptor protein expressed on the cells and is immobilized on a culture substrate and / or matrix molecule used for culturing the cells, the receptor protein mediates the transmission of extracellular signals to the cells based on its binding to the ligand molecule, and the extracellular signals are enhanced by the physical stimulation.

This structure constitutes a culture vessel having a plurality of accommodation sections in which cells are cultured. The structure comprises: a base that includes a plurality of through holes opening in the vertical direction; and an adherence layer which is provided on the bottom surface of the base and onto the bottom surface of which a sheet for closing the through holes is adhered. The adherence layer has openings that are located below the through holes and that have an outline greater than an outline of the through holes in plan view.

A flexible pouch assembly for cell culture includes one or more pouches forming a plurality of culture compartments wherein a drain port in at least a first culture compartment is adapted to fluidly connect with a second culture compartment when a valve arrangement is open. The invention also discloses a bioreactor for mounting the bag assembly on the rocking tray and a method for culturing cells in the assembly.

The disclosure pertains to a defined cell culture medium for the expansion of human retinal progenitors, comprising or consisting of a nutrient medium, a SHH-pathway activator and a GSK3 inhibitor. To the use of the defined cell culture medium for the expansion of retinal progenitors, as well as to an in vitro method for expanding retinal progenitors, comprising: (i) placing a culture of human retinal progenitors in a defined cell culture medium as defined in claims 1 to 8; and (ii) culturing the cells in said defined cell culture medium.

The present invention relates to the field of immune cell therapy, and specifically relates to a method for expanding NK cells and use thereof. The method comprises using OK-432 in combination with irradiated feeder cells, such as K562 cells, to expand and culture NK cells in vitro.

The invention provides a method for evaluating prebiotics and a compound combination thereof based on an in-vitro simulation fermentation model-co-culture cell model, and the method comprises the following steps: preparation of fecal bacteria suspension, preparation of an in-vitro fermentation culture medium, in-vitro fermentation culture and index determination of single prebiotics, and in-vitro fermentation culture and index determination of composite prebiotics. The method comprises the following steps: establishing a cell co-culture model, carrying out co-culture on composite prebiotics and cells, determining indexes, and determining optimal monomer prebiotics and composite prebiotics. By means of the method, the optimal prebiotic compound composition composed of the tea polyphenol, the fructo-oligosaccharide and the stachyose is obtained through screening for the first time, the antioxidant activity of the compound prebiotics and the synergistic anti-inflammatory effect of the short-chain fatty acid are proved, and the compound prebiotics are applied to preparation of the soft sweets.

The invention discloses an effect and mechanism research method of GOT1 in lung adenocarcinoma cells, and relates to the technical field of biomedical research. Comprising the following steps: S1, cell culture and protein extraction: culturing Bas-2b, A549, H1573 and Calu-3 cells, cleaning the cells with PBS, performing pyrolysis treatment, collecting supernate as a total protein solution, and determining the protein concentration per tube by BCA; s2, carrying out Western Blot experiment, taking 10 micrograms of protein as a loading amount, carrying out electrophoresis, transferring a film, sealing, incubating a primary antibody and a secondary antibody, exposing and developing, and storing a picture; the experiment is repeated for multiple times, and Image J is used for analyzing the strip exposure diagram. According to the method disclosed by the invention, effective knock-down of GOT1 in A549 and H1573 cells is successfully realized through the steps of screening proper cell screening concentration by puromycin, exploring an MOI value, transfecting lentivirus, screening puromycin and the like; the invention provides an important tool and method for follow-up research on the function of GOT1 in lung adenocarcinoma cells.

1. The name of the design product: cell culture bottle. 2. The use of the design product: for containing and culturing cells. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.