611 results about "Cultured cell" patented technology

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention provides a gamma delta T cell preparation as well as a preparation method and application thereof, and belongs to the technical field of T cells. The liposome is prepared from the following raw materials in parts by weight: 15-20 parts of modified gamma delta T cell liposome, 1-3 parts of cell stimulating factors and 4-7 parts of culture medium freeze-dried powder, the modified gamma delta T cell lipidosome is prepared by embedding gamma delta T cells through lipidosome, coupling with acetylenic bond modified chitosan, and further mixing with MFAP4 protein and b4GALT1 protein. The culture medium freeze-dried powder is prepared by freeze-drying the culture medium in the engineering culture process of gamma delta T cells, and the cell stimulating factors are resveratrol and quercitrin. According to the gamma delta T cell preparation prepared by the invention, the survival ability and resistance of gamma delta T cells are improved, the anti-tumor activity of the gamma delta T cells is improved, and the gamma delta T cell preparation has relatively good antioxidant and anti-inflammatory effects, reduces the inhibition of inflammation on an immune system and has a very good treatment effect on pancreatic ductal adenocarcinoma.

The invention relates to methods for culturing cells, and cultured animal cells for use in described method. Also provided is a modified animal cell and guide RNAs targeting genes to be modified in the modified and cultured cells of the invention.

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

This disclosure describes methods for improving umami and meaty flavors of cell-based food products. In particular, the disclosed method includes an amino acid integration method where cultured cells and exogenous amino acids (e.g., glutamic acid and aspartic acid) are combined and heated, resulting in a synergistic improvement of umami flavors. The disclosed method further includes a browning method by which a cell suspension is dehydrated and heated at high temperatures (e.g., greater than 140C) to induce non-enzymatic browning and caramelization reactions in the cell suspension. The cell-based flavoring product can be combined with a food product as a flavor, aroma, and color enhancer. The disclosed methods may use one or both of the amino acid integration method and the browning method to improve organoleptic properties of cell-based food products.

A method of culturing cells, the method including providing a microfluidic device including a substrate including an array of grooves that are substantially parallel; introducing cells into one or more of the grooves; providing a flow of culture medium over the groove array in a first flow direction that is across the grooves; culturing the cells under conditions to support viability, growth or differentiation, optionally wherein: (i) the cells are introduced into one or more grooves by flow over the groove array in the first flow direction; and / or (ii) the flow of culture medium is provided at a flow rate equivalent to 0.2-5 mL per million cells per day; and / or (iii) the cells are cultured for 1-14 days.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

The invention discloses a culture medium composition for culturing GDT cells and a culture method, and belongs to the field of biological medicine. Aiming at the problems of low in-vitro amplification efficiency and poor function of the existing GDT cells, the invention provides the culture medium composition for culturing the GDT cells, the culture medium composition comprises an induction culture medium and a proliferation culture medium, the induction culture medium comprises a basic culture medium and an activating agent, and the activating agent comprises IL-7 and paclitaxel; the proliferation culture medium comprises a basic culture medium, IL-18 and a metabolism regulator. An STAT5 pathway is activated through IL-7, and amplification of the Vdelta2 subgroup is specifically promoted; on the other hand, through the cooperation of the IL-7 and the IL-18, the IL-18 activates the expression of the NKG2D, and the IL-18 and the IL-7 jointly enhance the cytotoxicity; efficient amplification and function enhancement of the GDT cells are achieved; the killing rate of PD-L1 + tumor cells is larger than 80%, the motility rate after cryopreservation recovery is larger than 90%, the function maintenance rate is larger than 85%, and the cell line is suitable for tumor immunotherapy, infectious disease treatment and cell drug development.

The invention provides a preparation method of high-purity extracellular vesicles, and belongs to the technical field of extracellular vesicles, and the preparation method comprises the following steps: collecting cell culture supernatant generated by culturing cells, and setting a process parameter combination based on an orthogonal test principle; centrifuging the cell culture supernate for the first time; carrying out secondary centrifugation on the first centrifugal supernatant; filtering and purifying the first extracellular vesicles to obtain second extracellular vesicles; purifying the second extracellular vesicle to obtain a third extracellular vesicle; concentrating the third extracellular vesicles to obtain fourth extracellular vesicles; performing characteristic detection on the fourth extracellular vesicles, and recording detection data; establishing a preparation process physical and chemical model; performing parameter fitting on the preparation process physical and chemical model to obtain an optimal process parameter combination; carrying out an extracellular vesicle preparation experiment by adopting the optimal process parameter combination, and correcting a preparation process physical and chemical model to obtain a final process parameter combination; and preparing the extracellular vesicles.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

Provided herein are methods for producing a cultured tissue that mimics a natural tissue or organ. In some embodiments, the methods comprise culturing cells in a three-dimensional (3D) pattern on a scaffold, and applying an exogenous force to the cells while the cells are cultured. The applied exogenous force induces the cultured cells to form a cultured tissue that mimics the 3D cellular pattern and biomechanics of the natural tissue or organ. Also provided herein are bioreactors for producing a cultured tissue that mimics a natural tissue or organ. In certain embodiments, the bioreactors comprise: a scaffold for culturing cells in 3D pattern, and an exogenous force applicator to exert an exogenous force to the cells cultured in the 3D pattern. Additional embodiments provide cultured tissues that mimic natural tissues or organs as produced according to the methods disclosed herein.

The invention relates to a multifunctional integrated organoid culture device. The device comprises a main body, and a liquid input module, a temperature control module and an organoid module which are fixed on the main body, the main body comprises an adjusting assembly and a main control chip which are connected with each other, and the main control chip is connected with the temperature control module and the liquid input module and used for adjusting the flow speed of the liquid input module and the temperature of the temperature control module; the liquid input module is connected with the organ-like culture module and used for conveying cell sap to the organ-like culture module, the organ-like module is provided with a cell anchoring array and used for fixing and culturing cells, and the temperature control module is located between the liquid input module and the organ-like module and used for controlling the temperature of the organ-like culture module. The temperature detection module is used for detecting the temperature of the liquid input module and adjusting the temperature of the organ-like module. Compared with the prior art, the invention has the advantages of high control precision, multi-cell fusion, abundant functions and the like.

The invention discloses a ctEVs separation and analysis method with time resolution capability and application thereof. The ctEVs separation and analysis method comprises the following steps: S1, culturing cells by adopting glycometabolism markers; or injecting the animal by adopting a glycometabolism marker; step S2, manufacturing a fishbone-shaped micro-fluidic chip modified by streptavidin; s3, separating and analyzing the new tumor source extracellular vesicles by the micro-fluidic chip, and co-incubating the extracellular vesicles and the aptamer probe co-modified by Biotin and DBCO to realize biotinylation of the new tumor source extracellular vesicles; the aptamer probe is a heterologous multivalent aptamer probe; step S4, capturing biotin-labeled target extracellular vesicles by adopting a chip; and S5, acquiring a chip image by using a fluorescence microscope, and analyzing the concentration of the target extracellular vesicles in the sample according to a fluorescence signal. The method can be used for screening tumor circadian rhythm markers and the like.

The present invention provides a method for producing spheroids and cellular by-products of spheroids, such as extracellular vesicles. The method comprises providing a bioreactor having a cavity for culturing cells; inserting a cell into the cavity to form a spheroid; and perfusing a cell culture medium through the cavity to culture the spheroid. A porous scaffold may be soluble or insoluble, and the cells are inoculated into pores of the porous scaffold, where the cells aggregate in the pores of the porous scaffold to form spheroids. The bioreactor is continuously perfused for large scale spheroid production and enables collection of spheroids and / or extracellular vesicles or other components from cell secretory groups.

The invention provides a method for obtaining GiNK cells through novel culture medium amplification, a large number of highly purified human natural killer (NK) cells are obtained by combining IL-15 active fragments, PI3K inhibitors, key receptors targeting the surfaces of the NK cells, antibodies enhancing activation of the NK cells and various cytokines, and the GiNK cells can be used for preparing the human natural killer cells. Expansion from human peripheral blood mononuclear cells (PBMCs) can be achieved without the use of feeder cells. Through culture for 2-3 weeks, the GiNK cells can be amplified by hundreds to thousands of times, and the proliferation efficiency, functional activity and anti-tumor effect of the GiNK cells are remarkably improved. The culture medium provided by the invention not only reduces the use concentration of cell factors, but also solves the problem of function depletion of GiNK cells in in-vitro culture through the synergistic effect of various additives, and provides important technical support for clinical application of the GiNK cells.

Provided are a modified cell and the use thereof. The present invention specifically relates to a method for culturing a cell, which method comprises reducing the expression and / or weakening the activity of a target gene of the cell. The present invention further relates to a method for preventing and / or treating a tumor by using the cultured cell.

Apparatus, systems and methods for the adaptive passage of a culture of cells and apparatus and methods for dissociating cell colonies are described. The systems may include an imaging module, a pipette module, a handling module, and / or a stage module. Coordinated operation of the modules, optionally in an automated manner, is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at more than one time point. A first apparatus for adaptive passage of a culture cells includes an imaging module and at least one processor, which apparatus may be included in the systems or used in the methods. A second apparatus for dissociating cell colonies, may also be included in the systems or used in the methods, includes impact bumper(s) collidable with impact bracket(s) to transmit a dissociative force to a culture of cells.

The invention discloses a method for culturing NK (Natural Killer) cells. The method comprises the following steps: inoculating a mononuclear cell into a T25 cell culture flask, wherein the T25 cell culture flask is coated by a coating factor in advance; carrying out culture treatment on the inoculated mononuclear cells in a culture system containing an NK cell culture medium containing an activation amplification factor and plasma so as to obtain NK cells; wherein the coating factor, the activation amplification factor and the NK cell culture medium are derived from an NK cell amplification kit. According to the method provided by the embodiment of the invention, the dosage of the amplification reagent can be reduced, the amplification multiple of the NK cells is improved, and the production cost is reduced.

A method for in situ differentiation of stem cells within a cell culture bioreactor is provided. The method includes providing a bioreactor vessel having a cell culture chamber, the cell culture chamber having an inlet for introducing fluid into the cell culture chamber and an outlet for introducing fluid out of the cell culture chamber. The bioreactor vessel also includes a cell substrate disposed within the cell culture chamber for culturing cells on the cell substrate. The method includes seeding undifferentiated stem cells onto the cell substrate within the cell culture chamber and perfusing the cell culture chamber with a differentiation medium to promote differentiation of the undifferentiated stem cells into specific cell lineages, thereby causing the undifferentiated stem cells to become differentiated cells.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

1. Name of the product of this design: Cell culture vessel. 2. Purpose of this design product: This product is a container for culturing cells. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1. 5. Other situations that require explanation Other explanations: The container of this product is made of light-transmitting material. Reference Figure 2 shows a state where the lid is placed on the bracket and tightened, and reference Figure 3 shows a state where the bracket is moved to a position below the product, thereby stably supporting the container body.

A cell culture incubator has an incubator cabinet with an internal chamber for the incubation of cells in one or more cell culture vessels and a door opening from an external environment to the internal chamber. At least two imagers configured for imaging the cells are within the internal chamber and a manipulator for manipulating the cells in the one or more cell culture vessels is within the internal chamber. A cell culture vessel transfer device moves the one or more cell culture vessels between the at least two imagers within the internal chamber and a controller is configured for controlling the cell culture transfer device to move cell culture vessels between the at least two imagers.

The invention belongs to the field of biological medicine, and relates to a method for preparing iTNK cells, prepared iTNK, a pharmaceutical composition of the iTNK and application of the iTNK. Specifically, the invention relates to a method for preparing iTNK cells, which comprises the following steps: (1) adding a proper amount of anti-CD3 / CD28 / CD2T cell Activator into a culture medium for culturing T cells; culturing for 1-4 days; preferably culturing for 2 days; (2) replacing the culture medium with a culture medium which does not contain anti-CD3 / CD28 / CD2T cell Activator, and continuously culturing for 20 to 30 days; preferably, the culture lasts for 26 days. The iTNK cell prepared by the method disclosed by the invention has dual attributes of cytotoxic T cells and NK cells and a tumor killing function, and has a good anti-tumor application prospect.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

A cell culture matrix for culturing cells in a fixed bed reactor is provided. The cell culture matrix includes a first substrate material having a regular and orderly array of openings passing through the layer, the openings being separated by a substrate material having a physical structure that is substantially regular and uniform and configured such that cells grow thereon. The physical structure and array of the openings are configured such that when the first substrate material is rolled into a roll-shaped substrate matrix including a roll-shaped layer of the first substrate material, the variation in the packing density of the roll-shaped layer is less than about 20 times, less than about 10 times, less than about 5 times, or less than about 2 times throughout the roll-shaped substrate matrix.

To provide a polypeptide complex that exhibits cytotoxicity to a target cancer cell by T cells, a method for preparing the polypeptide, a cytotoxicity-inducing therapeutic agent comprising the polypeptide complex as an effective ingredient, as well as a pharmaceutical composition including the cytotoxicity-inducing therapeutic agent as an effective ingredient for treating or preventing various cancers or a therapeutic method using the pharmaceutical composition.SOLUTION: The invention provides a polypeptide complex which comprises: (1) an antigen-binding domain; (2) a domain comprising an Fc region with reduced Fcγ receptor-binding activity; and (3) a T cell receptor complex-binding domain. The invention also provides: a cell comprising the vector comprising the polynucleotide encoding the polypeptide complex; a method for preparing the polypeptide complex, comprising culturing the cell to harvest the polypeptide complex from the culture supernatant; a cytotoxicity-inducing therapeutic agent comprising the polypeptide complex as an effective ingredient; and a treating method comprising administering the therapeutic agent to a patient in need thereof.SELECTED DRAWING: None

The invention relates to the technical field of biology, and discloses a construction method of a gastric cancer organoid culture system, which comprises the following steps: preparation of a conditioned medium: culturing L-WRN cells and collecting the conditioned medium; preparing a gastric cancer organoid culture medium, and mixing the conditioned culture medium, the basic culture medium, a plurality of growth factors and additives; and extraction and culture of organoid: separating cells from gastric cancer tissues, mixing the cells with matrigel, and culturing by using the prepared culture medium. According to the method, the repeatability and the stability of the organoid model are improved, the response consistency of the organoid in drug screening is improved, and the reliability of the model in disease mechanism research and personalized medical application is guaranteed.

The invention relates to the technical field of biological cell culture media, and discloses a culture medium additive and a culture medium capable of improving the cell caking condition in the CHO cell culture process, and a method for culturing CHO cells in vitro by using the culture medium. According to the serum-free culture medium for culturing the CHO cells, calcium, magnesium and phosphorus substances with the original concentration and calcium, magnesium and phosphorus with the adjusted concentration are additionally added, the culture medium is optimized, the cell caking condition in the CHO cell culture process is improved, and the serum-free culture medium for culturing the CHO cells is simple in component, low in cost and free of protein and serum. When the optimized culture medium is used for culturing to the seventh day, the cell caking rate can be reduced from 37.15% to 18.38%, and the cell density can be increased from 4.56 * 10 < 6 > cells / mL to 17.4 * 10 < 6 > cells / mL.

Disclosed herein is a cell culture device (1) for culturing cells and / or organoids, the cell culture device comprising a cell containment unit (2) comprising at least one cell culture chamber (3) configured to contain a liquid cell culture medium (9) and a cell structure, wherein the cell culture chamber (3) comprises: a gas vent opening (4) configured to vent the cell culture chamber; a pressure chamber (5) comprising a pressure chamber inlet (6); a channel (7) connecting the at least one cell culture chamber (3) and the pressure chamber (5); the cell culture device further comprising a first pressure generator (8), in particular a pump, connected to the pressure chamber inlet and configured to temporarily pressurize the pressure chamber with a pressure pulse; wherein the at least one cell culture chamber (3), the pressure chamber (5) and the channel (7) are configured such that the pressure pulse from the first pressure generator (8) propagates from the pressure chamber (5) through the channel (7) to the at least one cell culture chamber (3) and in particular into the liquid cell culture medium (9).