69 results about "Cultured cell" patented technology

Disclosed herein is a cell culture device (1) for culturing cells and / or organoids, the cell culture device comprising a cell containment unit (2) comprising at least one cell culture chamber (3) configured to contain a liquid cell culture medium (9) and a cell structure, wherein the cell culture chamber (3) comprises: a gas vent opening (4) configured to vent the cell culture chamber; a pressure chamber (5) comprising a pressure chamber inlet (6); a channel (7) connecting the at least one cell culture chamber (3) and the pressure chamber (5); the cell culture device further comprising a first pressure generator (8), in particular a pump, connected to the pressure chamber inlet and configured to temporarily pressurize the pressure chamber with a pressure pulse; wherein the at least one cell culture chamber (3), the pressure chamber (5) and the channel (7) are configured such that the pressure pulse from the first pressure generator (8) propagates from the pressure chamber (5) through the channel (7) to the at least one cell culture chamber (3) and in particular into the liquid cell culture medium (9).

A method of coating a cell culture substrate in situ in a bioreactor is provided that includes providing a bioreactor vessel having a cell culture chamber with an inlet for flowing fluid into the cell culture chamber and an outlet for flowing fluid out of the cell culture chamber, and a cell substrate disposed in the cell culture chamber for culturing cells. The method includes providing a coating solution for coating the cell substrate; inputting the coating solution into the cell culture chamber such that the coating solution contacts the cell substrate; and removing an excess of the coating solution from the cell culture chamber. After removing the coating solution, a coated cell substrate remains in the cell culture chamber.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The application relates to the technical field of cell culture, in particular to a method for efficiently culturing NK cells in vitro and research on the antitumor activity of the NK cells in vitro. The method comprises the following steps: on day 0, resuspending PBMCs with a first-stage activation culture medium and culturing for 20-25 hours; on day 1, supplementing a second-stage activation culture medium and continuing to culture for 45-50 hours; on day 3 and day 5, counting the cells, respectively supplementing a third-stage culture medium, and culturing to day 7; on day 7, counting the cells, supplementing a fourth-stage culture medium, and culturing and standing; on day 9, day 11 and day 13, respectively supplementing the fourth-stage culture medium, and culturing to day 15 for harvesting. The method is simple in operation, low in cost, high in quantity of obtained NK cells, high in purity of the NK cells and high in killing activity of the NK cells.

The objective is to provide a novel method for improving the efficiency of osteogenic differentiation induction based on immobilized BMP-2. [Solution] A method for stimulating cells with ligand molecules, comprising a stimulation step of applying physical stimulation to the cells and / or ligand molecules, wherein the ligand molecule is bound to or interacts with a receptor protein expressed on the cells and is immobilized on a culture substrate and / or matrix molecule used for culturing the cells, the receptor protein mediates the transmission of extracellular signals to the cells based on its binding to the ligand molecule, and the extracellular signals are enhanced by the physical stimulation.

To provide a scaffolding material for cell culture that has excellent transparency and can be used in scaffold-type cell culture, particularly a scaffolding material for cell culture that can form a scaffolding material with a hydrogel pattern using a simple method by exposure and development.SOLUTION: Provided is a scaffolding material for cell culture containing a fibrous cellulose with an average fiber width of 100 nm or less, and a negative radiation-sensitive component containing a polymerizable compound and a polymerization initiator, the content of the negative radiation-sensitive component being 20 to 200 pts.mass based on 100 pts.mass of the fibrous cellulose, the negative radiation-sensitive component being a component that is soluble in at least one selected from water, alcohol, and a mixed solvent thereof.SELECTED DRAWING: None

This invention belongs to the field of organoid culture technology, specifically involving BCL2 Application of genes in 3D cluster culture of stem cell-derived organoids. Starting on day 4 of bovine embryonic stem cell cluster culture, the cell cluster edges became indistinct, cells began to die and darken, and the cell clusters began to diffuse. By day 7, the cell cluster survival rate was only 14.2%. This invention utilizes the overexpression of genes in bovine embryonic stem cells... BCL2 Following gene expression, the cells were cultured in clusters using the same method. The overexpressed cell clusters showed clear edges, and their volume increased continuously with the number of culture days. Even on day 7, clear cell cluster edges were still observable, and no diffuse cell death was observed; the survival rate remained high at 85.54%. BCL2 Genes can improve the survival rate of early cultured cell clusters of organoids, which is of great significance for the normal morphogenesis and functional maturation of organoids and can greatly improve the efficiency of organoid preparation.

The present invention addresses the problem of providing a novel method for increasing bone differentiation induction efficiency based on immobilized BMP-2.　Provided is a method for stimulating a cell with a ligand molecule, the method including a stimulation step for applying a physical stimulus to the cell and / or the ligand molecule. The ligand molecule binds to or interacts with a receptor protein expressed on the cell, and is immobilized on a culture substrate and / or a matrix molecule used for culturing the cell. The receptor protein mediates the transmission of an extracellular signal to the cell on the basis of binding to the ligand molecule, and the extracellular signal is enhanced by the physical stimulus.

This invention discloses a vascular organ-on-a-chip that simulates human blood pressure, comprising a substrate, a channel layer, a cell dynamic culture module, a solution mixing module, and a cell mechanical property measurement module. The cell dynamic culture module includes a first culture channel and a second culture channel, with a serpentine channel connecting the upstream of the first culture channel and the upstream of the second culture channel. The solution mixing module includes two oppositely arranged solution mixing channels, which are respectively connected to the first culture channel and the second culture channel. The cell mechanical property measurement module includes multiple measurement channels arranged side-by-side, with one end of each measurement channel connected to the other end of one solution mixing channel and the other end connected to the other end of another solution mixing channel. This invention simulates the human blood pressure environment within the vascular organ-on-a-chip, enabling simultaneous cell culture under parallel gradient pressure within the chip, and combining the vascular organ-on-a-chip with cell mechanical property measurement.

This invention, entitled "A Method and Product for Pretreatment of Mesenchymal Stem Cells and Its Application in Stroke Drugs," belongs to the field of biotechnology. The technical problem to be solved is to improve the function and therapeutic effect of mesenchymal stem cells. The key technical solution is a method for pretreatment of mesenchymal stem cells, including: culturing cells using an activation medium, culturing cells using a directional medium, and culturing cells under hypoxic conditions using an adaptation medium; the activation medium contains complete medium, IFN-γ, TNF-α, and IL-1β; the directional medium contains complete medium, sodium valproate, 5-azacytidine, and all-trans retinoic acid; the adaptation medium is a complete medium containing basal medium and free of animal serum. The pretreatment method of this invention enhances the anti-inflammatory, neuroprotective, and angiogenic capabilities of mesenchymal stem cells, showing significant therapeutic effects on stroke, and since it does not use animal serum, it meets clinical requirements.

This invention discloses a method for large-scale expansion of MSC cells using 3D culture, comprising the following steps: MSC seed cell resuscitation; MSC seed cell planar culture; MSC seed cell planar expansion; MSC seed cell concentration and washing; primary scale-up culture of MSC seed cells; MSC cell washing and concentration; MSC seed cell scale-up culture; microcarrier lysis and cell suspension collection; MSC cell washing and concentration; MSC cell aliquoting and cryopreservation. The advantages of this invention are: it achieves highly efficient scale-up of MSC cells from 2D planar culture to a 3D microcarrier bioreactor, enabling cell expansion to reach more than 20-fold (primary scale-up culture) to more than 200-fold (secondary scale-up culture), while maintaining cell viability >95%.

The invention discloses a co-culture method of cell culture meat and application of the co-culture method in improving meat quality and flavor. According to the invention, through two-stage differentiation, muscle precursor cells and fat precursor cells are respectively subjected to proliferation culture, then are separately subjected to primary differentiation, and are mixed together for co-culture, and meanwhile, second-stage continuous differentiation is carried out; the problem that differentiation of muscle cells and fat cells cannot be effectively and simultaneously differentiated under the co-culture condition due to different requirements of differentiation of muscle cells and fat cells on the serum environment is solved. In addition, the invention also provides an objective standard for evaluating the flavor quality of cell culture meat generated by co-culture. The cell culture meat with different flavors can be obtained by adjusting the proportion of the co-culture cells through the co-culture method, and the flavor quality of the cell culture meat from other sources can be evaluated and detected through the amino acid detection means.

This invention discloses a microfluidic chip and its fabrication method for constructing modular cell cultures, relating to the field of cell culture technology. The chip includes an upper plate and a lower plate. The upper plate includes a first flow channel and a second flow channel, while the lower plate includes multiple spaced-apart culture chambers, each with a different cross-sectional shape. This allows each chamber to be independently formed into cultures of different shapes. The cultures can be stacked and combined after removal to construct a multi-cell co-culture model with functional partitions, significantly improving structural complexity and biomimicry. The upper plate slides to switch the connection between the first and second flow channels and the culture chambers, enabling inoculation and culture modes. During inoculation, cultured cells are injected into the culture chamber through the first flow channel; during culture, the flow channel is switched to perfuse the culture chamber with culture medium, avoiding damage to the culture during residual gel removal. This simple operation improves the success rate and enhances the reliability of experimental results.

This application proposes a method for three-dimensional cell culture using an injectable thermosensitive hydrogel. Cells are mixed with a thermosensitive hydrogel precursor solution and incubated at 34-37°C for 1-10 minutes, followed by the addition of cell culture medium. After culture, a vitamin B6 derivative is added for degradation, yielding a cell suspension. The cultured cells are then separated by centrifugation. Using a hydrogel with a rapid sol-gel phase transition provides cells with a three-dimensional support environment close to the in vivo environment, enabling cells to grow, proliferate, differentiate, and interact with other cells and the matrix under similar physiological conditions, achieving high-quality in vitro cell culture. Specifically, the three-dimensional structure is less affected by temperature during culture, which is beneficial for stable cell culture. After culture, the gel structure degrades rapidly and completely, allowing for rapid cell recovery; this is a high-quality cell culture method.

1. The name of the design product: stirring bioreactor. 2. The use of the design product: the design product is a stirring bioreactor for culturing cells in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application belongs to the technical field of cell culture, and particularly relates to a temperature-sensitive cell three-dimensional culture support, which comprises temperature-sensitive hydroxypropyl chitin, the molecular weight of the temperature-sensitive hydroxypropyl chitin is 15,000-300,000 Da, and the degree of deacetylation (DD) is 35%-65%. The support material is temperature-sensitive hydroxypropyl chitin prepared by derivatization of chitosan of a natural source through a specific process, and can be used for three-dimensional culture of cells. The temperature-sensitive hydroxypropyl chitin is used for three-dimensional culture of cells, and can significantly reduce the culture cost, improve the convenience and the viability of the cultured cells.

In a surface-enhanced Raman scattering-based method for testing drug reactivity of cerebrovascular cells, a SERS substrate is manufactured. A SERS substrate array including a plurality of the SERS substrates is manufactured. Cells are cultured on the SERS substrate array. A drug is administered to the cultured cells. A Raman signal is measured from the cells to which the drug is administered. An optimal drug is selected from among the administered drugs on the basis of the measured Raman signal.

The present invention provides rolled scaffold devices and cell culture systems that can provide a large surface-area-to-volume ratio for expanded cell culture. The rolled scaffolds minimize shear stress on cultured cells and support sufficient and uniform mass transfer rates of gases and nutrients. The rolled scaffolds can be connected to a media source via holders to support large-scale expansion and maintenance of cell cultures.

A chip for cell culture, including a laminate having a flow path structure inside, in which the laminate includes a bottom plate substrate, a flow path substrate in which a flow path is formed by laser processing, and a top plate substrate in this order, and the flow path substrate includes a resin having a glass transition temperature of 37°C or higher, an elastic modulus at 25°C of 1 × 109 Pa or more, and an elastic modulus at 150°C of 1 × 107 Pa or less.

Microfluidic device for culturing cells A method for forming a microfluidic device for culturing cells, the method comprising: providing a microfluidic device comprising a first chamber and a second chamber fluidically connected to the first chamber by a first porous structure comprising through holes for allowing fluid to pass through, said first porous structure physically separating the first chamber and the second chamber, wherein each of said chambers has photosensitive inner walls having a first property; and then irradiating the microfluidic device with light to which the photosensitive inner walls are sensitive, in such a way that the first porous structure acts as a mask during irradiation, thereby changing the surface property of the exposed surfaces and imparting a different surface property in the first chamber compared to the second chamber.

The present application relates to a kind of fusion immunoglobulin and its application in culture T cell, belong to biological medicine technical field.The present application provides a kind of fusion immunoglobulin, the fusion immunoglobulin is the immunoglobulin of fusion IL-2 protein fragment, IL-21 protein fragment and IL-15 protein fragment.The fusion immunoglobulin constructed will IL-15, IL-21 and IL-2 be delivered together, effectively simulate in-vivo environment, promote T cell proliferation, induced T cell has high killing, low exhaustion and strong memory and so on excellent phenotype, significantly prolong cytokine half-life.The fusion immunoglobulin of the present application is added in culture medium, and the fusion protein culture medium is prepared, avoids animal source serum component, batch consistency is excellent.The fusion immunoglobulin in culture medium can be accurately, synergistically and long-acting stimulation to T cell.

The present application relates to the field of biomedical engineering, and particularly relates to a pump-free intestinal chip system capable of measuring transmembrane resistance value and a measuring method thereof.The system comprises transmembrane resistance measuring electrodes, an intestinal chip and a swing-type shaker; the intestinal chip is composed of a plurality of chip units arranged in parallel, and each chip unit comprises, from top to bottom, a culture layer, a porous membrane and a perfusion layer; the culture layer is provided with a first end hole, an intermediate hole and a second end hole in sequence, both end holes are communicated with the cavity of the perfusion layer to form a fluid channel, the intermediate hole is used for culturing cells, and the intermediate hole separates the culture layer and the perfusion layer and exchanges materials through the porous membrane; the swing-type shaker is used for providing periodic gravity flow to simulate intestinal peristalsis; the transmembrane resistance measuring electrodes are connected with a resistance meter, and the transmembrane resistance value is obtained through current-voltage measurement.The present application has better simulation of intestinal barrier characteristics, can measure the transmembrane resistance value of the intestinal chip, and is convenient to operate and short in time consumption.

A bioreactor system for culturing cells is provided. The bioreactor system includes a vessel comprising an inlet, an outlet, and an internal cavity disposed between the inlet and the outlet and for perfusion of a cell culture medium therethrough. The system further includes a cell culture substrate disposed in the internal cavity in a fixed bed configuration, the surface of the cell culture substrate having a surface area for culturing cells thereon during operation of the bioreactor system. By adjusting the surface area of the cell culture substrate, the bioreactor system can be scaled up or down depending on the number of cells to be cultured.

The present disclosure relates to a composition for cell culture, a method for preparing the composition, a method for culturing cells using the composition, and cells obtained thereby. Using the composition for cell culture disclosed herein to culture cells can achieve effects including large-scale cell expansion, maintaining high cell viability, an early memory phenotype, and a low exhaustion phenotype.

The present application belongs to the field of biotechnology, and relates to a method for producing coxsackie virus by using HeLa cells. Specifically, the present application relates to a method for culturing HeLa cells in vitro to produce coxsackie virus, which comprises: sequentially culturing HeLa cells in culture medium III and culture medium I, inoculating virus and culturing to obtain a cell culture containing coxsackie virus; wherein the culture medium III is a mixture of culture medium II and VirusPro culture medium at a volume ratio of 3:1-1:1, the culture medium I is a mixture of culture medium II and VirusPro culture medium at a volume ratio of 3:1-1:3, and the culture medium III and the culture medium I contain L-alanyl-L-glutamine.

This utility model relates to the field of traditional Chinese medicine drying technology, specifically an energy-saving traditional Chinese medicine drying system. It includes a bottle body with a cap threaded to the top. The cap has vent holes and a sealing area. The vent holes are divided into multiple regions, each with a gradually increasing diameter. A rotating plate is rotatably mounted on the top of the cap, with a fan-shaped notch extending through its center. The cap's top features vent holes and a sealing area. Operators can rotate the rotating plate according to specific cell culture needs, aligning the fan-shaped notch with the required vent holes or sealing area. This satisfies diverse cell culture requirements without the need for multiple vent caps. Adjustment is simple and convenient. In closed mode, the sealing plug is removed, and a pipette is used to take samples through the sampling port. In this case, the cell culture environment inside the bottle needs to be isolated from the outside environment. Sampling through the smaller sampling port eliminates the need to open the cap, reducing the impact of the external environment on the cells inside the bottle.

The culture apparatus of the present invention is a culture apparatus configured within a culture environment generating device during cell culture, comprising: a swinging unit that swings a culture container containing cultured cells and culture medium; a liquid delivery unit that moves the culture medium between a supply container, a culture container, and a discharge container; a detection unit that detects relevant information about the culture medium contained in the culture container; and a communication unit that transmits the relevant information about the culture medium to an external terminal located outside the culture environment generating device.

A modified cell and its use are provided, specifically relating to a method of culturing cells comprising reducing the expression and / or weakening the activity of a target gene in the cells; and also providing a method of using the cultured cells to prevent and / or treat tumors.

A method of using a cell culture bioreactor for differentiating stem cells in situ in the bioreactor is provided. The method includes providing a bioreactor vessel that has a cell culture chamber with an inlet for flowing fluid into the cell culture chamber and an outlet for flowing fluid out of the cell culture chamber. The bioreactor vessel also includes a cell substrate disposed in the cell culture chamber for culturing cells thereon. The method includes seeding undifferentiated stem cells onto the cell substrate in the cell culture chamber and perfusing the cell culture chamber with a differentiation media to promote differentiation of the undifferentiated stem cells into a specified cell lineage, whereby the undifferentiated stem cells become differentiated cells.