787 results about "Cultured cell" patented technology

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention relates to the technical field of microbial culture, and discloses a cell culture device, which comprises: a plate body; the culture units are arranged on the plate body at intervals in the width direction of the plate body, and each culture unit comprises a plurality of base holes which are arranged at intervals in the length direction of the plate body and are used for culturing cells and fluid channels which are arranged between every two adjacent base holes; according to the flowing direction of the culture medium along the fluid channels, the two sections of fluid channels corresponding to the two upward sides of each base hole are called the upstream fluid channel and the downstream fluid channel of the base hole; a downstream hole wall is arranged at least between the fluid channel at the downstream of the base hole and the base hole, and a plurality of downstream gaps allowing the culture medium to pass through and limiting the cells to pass through are formed in the downstream hole wall in a cutting manner.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention provides a gamma delta T cell preparation as well as a preparation method and application thereof, and belongs to the technical field of T cells. The liposome is prepared from the following raw materials in parts by weight: 15-20 parts of modified gamma delta T cell liposome, 1-3 parts of cell stimulating factors and 4-7 parts of culture medium freeze-dried powder, the modified gamma delta T cell lipidosome is prepared by embedding gamma delta T cells through lipidosome, coupling with acetylenic bond modified chitosan, and further mixing with MFAP4 protein and b4GALT1 protein. The culture medium freeze-dried powder is prepared by freeze-drying the culture medium in the engineering culture process of gamma delta T cells, and the cell stimulating factors are resveratrol and quercitrin. According to the gamma delta T cell preparation prepared by the invention, the survival ability and resistance of gamma delta T cells are improved, the anti-tumor activity of the gamma delta T cells is improved, and the gamma delta T cell preparation has relatively good antioxidant and anti-inflammatory effects, reduces the inhibition of inflammation on an immune system and has a very good treatment effect on pancreatic ductal adenocarcinoma.

The invention relates to a method for rapidly detecting the cell adhesiveness of probiotics and application of the method. The method comprises the following steps: adding the probiotics into adherent culture cells, incubating, discarding liquid, digesting and collecting the cells by pancreatin, extracting total DNA (Deoxyribonucleic Acid), performing qPCR (Quantitative Polymerase Chain Reaction) amplification, and calculating the adhesion quantity of the probiotics on a single cell according to a Ct value. The invention provides a method for rapidly detecting the cell adhesion rate of probiotics by using a qPCR (quantitative polymerase chain reaction) technology, which comprises the following steps of: extracting total genome DNA (deoxyribonucleic acid) after the probiotics are adhered to cells on the basis of the gene expression difference of prokaryotic and eukaryotic cells, and detecting the specific gene expression level of the probiotics and the cells by using the qPCR technology. The cell adhesion ability of the probiotics is evaluated by calculating the ratio of the gene expression quantities of the two, and the method has the characteristics of high efficiency, rapidness, high accuracy and the like.

The invention relates to methods for culturing cells, and cultured animal cells for use in described method. Also provided is a modified animal cell and guide RNAs targeting genes to be modified in the modified and cultured cells of the invention.

The invention discloses a cell preparation treatment method, system and medium, and the method comprises the following steps: carrying out cell amplification treatment on a to-be-cultured cell sap in a cell culture container to obtain a cultured cell sap; washing and concentrating the cultured cell sap in the cell culture container to obtain concentrated cell sap; and sub-packaging the concentrated cell sap in the cell culture vessel into a plurality of preparation bags. According to the invention, cell culture, cell washing and concentrating treatment and preparation sub-packaging are realized on the cell culture container, so that scattered arrangement of equipment is avoided, and the occupied space is reduced; the steps are sequentially completed through the cell culture container, the whole process from cell culture to cell preparation can be ensured to be performed in a sealed sterile environment, cell sap does not need to be transferred, and the cell culture quality is improved; the steps are sequentially and integrally completed through the cell culture container, manual intervention is reduced, the whole process from cell culture to cell preparation is intelligently carried out, and the cell culture efficiency is improved.

1. The name of the product of this design: bioreactor. 2. Purpose of the product of this design: bioreactor for culturing cells. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The utility model relates to the technical field of cell culture, and discloses a cell culture bottle, comprising a culture bottle, wherein a bottom wall is arranged at the bottom of the culture bottle, a liquid-blocking and breathable membrane is arranged at the opening of the culture bottle, a closing cover is installed on the outside of the culture bottle, a fixing ring is fixedly connected to the outside of the culture bottle, a plug is arranged inside the fixing ring, and a sealing block is fixedly connected to the inside of the plug. The cell culture bottle has the advantages of being convenient for adding culture fluid and reducing the influence of the external environment on the internal environment of the culture bottle. The plug is used to close a pipeline, and the culture bottle is divided into two cavities by a partition plate, so that culture fluid can be added through the pipeline, and a blocking mechanism is opened by gravity to add culture fluid to the inside of the cavity for culturing cells, so as to complete the addition of culture fluid, thereby solving the problem that the external environment may contaminate cells when the closing cover is opened to pour the culture fluid through the opening during the current cell culture process.

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

The invention relates to methods and apparatus for culturing cells. There is provided methods and apparatus for providing a seed train comprising a chemostat, turbidostat or auxostat and methods and apparatus for medium exchange during culturing using gravitational settling separation.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a vascularized organ chip and method for large-scale culture, the vascularized organ chip comprises an upper runner layer and a lower culture layer, and the runner layer and the culture layer are fixedly sealed; a liquid storage pipe matched with the flow channel layer is adhered to the upper surface of the flow channel layer; according to the vascularized organ chip, the flow channel layer is used for pouring a culture solution to provide fluid stimulation and nutrients for cells; the three parallel-connected large-scale culture chambers of the culture layer are used for culturing cells, so that blood circulation of a microvascular system in a human body can be simulated, and a microenvironment in tissues can be reproduced more truly; meanwhile, the requirement for culturing a large number of tissue cells through the chip can be met, and the cell yield and the experiment scale can be effectively increased.

The invention provides a preparation method and application of mesenchymal stem cell exosomes, and the method is characterized in that N-acetylcysteine and quercetin are added into a culture medium used for culturing MSC cells, so that the MSC cells are stimulated to generate exosomes with anti-aging and anti-apoptosis effects. In addition, the exosome generated by stimulating the MSC through a culture mode of gradually reducing oxygen partial pressure further has an anti-aging effect; the adverse effects of acute oxidative stress, decline of multiplication capacity, unstable quality and the like in a low-oxygen state can be inhibited, and the activity of related components of cells and exosomes is not damaged. The method shows good anti-aging and anti-apoptosis effects in vitro, and a new thought is provided for treating aging diseases by the exosome in the future.

The invention provides an applicable population screening method and application of a glioma dry-like cell sensitized DC vaccine, peripheral blood of a patient before and after inoculation of the glioma dry-like cell sensitized DC vaccine is obtained, mononuclear cells are extracted, CD8 + T cells are respectively separated from the mononuclear cells through a magnetic bead sorting method, and the CD8 + T cells are separated from the mononuclear cells. The method comprises the following steps: uniformly inoculating CD8 < + > T cells into an ELISPOT cell culture plate coated with an IFN-gamma antibody for culture, respectively setting an experimental group and a positive control group for culture, adding A2B5 < + > glioma cell lysis buffer into the experimental group and adding phytohemagglutinin into the positive control group, and carrying out ELISPOT staining on the cultured cells to obtain the secretion amount of FN-gamma before and after treatment of a patient. According to the secretion amount of the FN-gamma, patients with specific immune response to the glioma dry-like cell sensitization DC vaccine are screened, so that the glioma dry-like cell sensitization DC vaccine is conveniently popularized and applied, and the immune mechanism of the glioma dry-like cell sensitization DC vaccine is researched.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a function-enhanced mesenchymal stem cell exosome, the preparation method comprises the following steps: S1, preparing an NK cell exosome, collecting supernate in an NK cell culture medium, and treating the supernate by using an optimized exosome separation and purification process to obtain an NK cell exosome; finishing preparation of a stock solution; s2, culturing the mesenchymal stem cells, culturing the cells in a 3D suspension culture mode, treating and controlling low oxygen concentration by combining with NK cell exosomes, and simulating an in-vivo inflammation microenvironment in a multi-azimuth manner, so that the exosomes secreted by the mesenchymal stem cells have a stronger immune regulation function and an anti-inflammatory effect; s3, preparation of mesenchymal stem cell exosomes: preparing a stock solution through an exosome separation and purification process combining a tangential flow ultrafiltration technology and a PS affinity chromatography technology; the exosome stock solution obtained by the method is high in purity and high in yield, and has more excellent biological activity and excellent treatment effect.

This disclosure describes methods for improving umami and meaty flavors of cell-based food products. In particular, the disclosed method includes an amino acid integration method where cultured cells and exogenous amino acids (e.g., glutamic acid and aspartic acid) are combined and heated, resulting in a synergistic improvement of umami flavors. The disclosed method further includes a browning method by which a cell suspension is dehydrated and heated at high temperatures (e.g., greater than 140C) to induce non-enzymatic browning and caramelization reactions in the cell suspension. The cell-based flavoring product can be combined with a food product as a flavor, aroma, and color enhancer. The disclosed methods may use one or both of the amino acid integration method and the browning method to improve organoleptic properties of cell-based food products.

The present disclosure provides methods for genetically engineering T cells, such as CD4+ T cells, for use in cell therapy. In some aspects, the provided methods include one or more steps for incubating the cells under stimulating conditions, introducing a recombinant polypeptide to the cells through transduction or transfection, and cultivating the cells under conditions that promote proliferation and / or expansion. In some aspects, the incubation and / or the cultivation is performed in the presence of recombinant IL-2. In some aspects, the provided methods are an efficient, reliable means to produce genetically engineered T cells with a high degree of success.

A method of culturing cells, the method including providing a microfluidic device including a substrate including an array of grooves that are substantially parallel; introducing cells into one or more of the grooves; providing a flow of culture medium over the groove array in a first flow direction that is across the grooves; culturing the cells under conditions to support viability, growth or differentiation, optionally wherein: (i) the cells are introduced into one or more grooves by flow over the groove array in the first flow direction; and / or (ii) the flow of culture medium is provided at a flow rate equivalent to 0.2-5 mL per million cells per day; and / or (iii) the cells are cultured for 1-14 days.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

The invention discloses a culture medium composition for culturing GDT cells and a culture method, and belongs to the field of biological medicine. Aiming at the problems of low in-vitro amplification efficiency and poor function of the existing GDT cells, the invention provides the culture medium composition for culturing the GDT cells, the culture medium composition comprises an induction culture medium and a proliferation culture medium, the induction culture medium comprises a basic culture medium and an activating agent, and the activating agent comprises IL-7 and paclitaxel; the proliferation culture medium comprises a basic culture medium, IL-18 and a metabolism regulator. An STAT5 pathway is activated through IL-7, and amplification of the Vdelta2 subgroup is specifically promoted; on the other hand, through the cooperation of the IL-7 and the IL-18, the IL-18 activates the expression of the NKG2D, and the IL-18 and the IL-7 jointly enhance the cytotoxicity; efficient amplification and function enhancement of the GDT cells are achieved; the killing rate of PD-L1 + tumor cells is larger than 80%, the motility rate after cryopreservation recovery is larger than 90%, the function maintenance rate is larger than 85%, and the cell line is suitable for tumor immunotherapy, infectious disease treatment and cell drug development.

The invention provides a preparation method of high-purity extracellular vesicles, and belongs to the technical field of extracellular vesicles, and the preparation method comprises the following steps: collecting cell culture supernatant generated by culturing cells, and setting a process parameter combination based on an orthogonal test principle; centrifuging the cell culture supernate for the first time; carrying out secondary centrifugation on the first centrifugal supernatant; filtering and purifying the first extracellular vesicles to obtain second extracellular vesicles; purifying the second extracellular vesicle to obtain a third extracellular vesicle; concentrating the third extracellular vesicles to obtain fourth extracellular vesicles; performing characteristic detection on the fourth extracellular vesicles, and recording detection data; establishing a preparation process physical and chemical model; performing parameter fitting on the preparation process physical and chemical model to obtain an optimal process parameter combination; carrying out an extracellular vesicle preparation experiment by adopting the optimal process parameter combination, and correcting a preparation process physical and chemical model to obtain a final process parameter combination; and preparing the extracellular vesicles.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention belongs to the technical field of cell culture, and discloses a multi-storage-module decidua uteri cell culture device which comprises a culture box body, a sealing cover plate is arranged at the upper end of the left side of the culture box body and rotationally connected with the culture box body, and a limiting sliding groove is formed in the inner wall of the culture box body; a placement supporting plate is arranged in the culture box body, limiting sliding blocks are connected to the two sides of the placement supporting plate, a placement groove is formed in the upper end of the placement supporting plate, a culture dish body is arranged in the placement groove, a guide groove is formed in the upper end of the left side of the culture box body, and a sealing plate is arranged in the guide groove. According to the decidua uteri cell culture device with the multiple storage modules, the sealing structure is arranged, the sealing cover plate is used for providing power, and when the sealing cover plate is opened, the sealing plate can be driven to move downwards to seal the taking opening, so that it is ensured that the internal temperature of the culture box body is not affected by the external environment to be changed; and stable growth of other cultured cells inside is ensured.

System and methods for continuous culture comprise performing a first continuous culture of cells in a chamber of a first bioreactor under culture conditions that produce cell growth; moving culture fluid comprising cells from the chamber of the first bioreactor into a chamber of a second bioreactor though one or more fluidic conduits; and performing, in the chamber of the second bioreactor, a second continuous culture of cells under culture conditions that produce at least one culture product. The process is performed under conditions wherein the rate of cell growth in the first continuous culture is greater than that of the second continuous culture, and the production of culture product in the first continuous culture is less than that of the second continuous culture.

Provided herein are methods for producing a cultured tissue that mimics a natural tissue or organ. In some embodiments, the methods comprise culturing cells in a three-dimensional (3D) pattern on a scaffold, and applying an exogenous force to the cells while the cells are cultured. The applied exogenous force induces the cultured cells to form a cultured tissue that mimics the 3D cellular pattern and biomechanics of the natural tissue or organ. Also provided herein are bioreactors for producing a cultured tissue that mimics a natural tissue or organ. In certain embodiments, the bioreactors comprise: a scaffold for culturing cells in 3D pattern, and an exogenous force applicator to exert an exogenous force to the cells cultured in the 3D pattern. Additional embodiments provide cultured tissues that mimic natural tissues or organs as produced according to the methods disclosed herein.

The invention relates to a multifunctional integrated organoid culture device. The device comprises a main body, and a liquid input module, a temperature control module and an organoid module which are fixed on the main body, the main body comprises an adjusting assembly and a main control chip which are connected with each other, and the main control chip is connected with the temperature control module and the liquid input module and used for adjusting the flow speed of the liquid input module and the temperature of the temperature control module; the liquid input module is connected with the organ-like culture module and used for conveying cell sap to the organ-like culture module, the organ-like module is provided with a cell anchoring array and used for fixing and culturing cells, and the temperature control module is located between the liquid input module and the organ-like module and used for controlling the temperature of the organ-like culture module. The temperature detection module is used for detecting the temperature of the liquid input module and adjusting the temperature of the organ-like module. Compared with the prior art, the invention has the advantages of high control precision, multi-cell fusion, abundant functions and the like.