1222 results about "Cultured cell" patented technology

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention relates to the technical field of microbial culture, and discloses a cell culture device, which comprises: a plate body; the culture units are arranged on the plate body at intervals in the width direction of the plate body, and each culture unit comprises a plurality of base holes which are arranged at intervals in the length direction of the plate body and are used for culturing cells and fluid channels which are arranged between every two adjacent base holes; according to the flowing direction of the culture medium along the fluid channels, the two sections of fluid channels corresponding to the two upward sides of each base hole are called the upstream fluid channel and the downstream fluid channel of the base hole; a downstream hole wall is arranged at least between the fluid channel at the downstream of the base hole and the base hole, and a plurality of downstream gaps allowing the culture medium to pass through and limiting the cells to pass through are formed in the downstream hole wall in a cutting manner.

The purpose of the present invention is to provide a culture medium in which cells to be used for producing a cultured meat can be grown, by adding thereto a food material as an additive. As a result of culturing cells using a food ingredient as an additive, it became possible to obtain high growth potential in fibroblasts and myoblasts by adding egg white or a dried product of egg white as a cell growth promoter. Accordingly, the present invention pertaining to a cell-growing culture medium containing a basic culture medium and egg white as a cell growth promoter was accomplished.

The invention belongs to the technical field of cell culture, and particularly relates to an intelligent chimeric cell culture cabin which is characterized by comprising an outer cabin body and a culture mechanism mounted above the outer cabin body, the fan rotates to suck air outside the device into the culture cabin through the air inlet, and in the process of blowing the air into the culture cabin, the heating pipe can heat the sucked air to increase the temperature of the air, so that the temperature of the air sucked into the device adapts to the temperature required by cell culture, and the situation that cell culture is affected by improper temperature is avoided; the sucked air is discharged out of the device through the air outlet after being circulated in the culture cabin body, so that the air in the culture cabin body can be circulated, meanwhile, waste gas generated by culturing cells in the culture cabin body is taken away, and angle openings of the air dissipation openings are staggered, so that the air discharged from the air dissipation openings can face different angles, and the air dissipation effect is improved. And the situation that the blown airflow faces the same angle and is too intense is avoided.

The invention relates to a cell culture microcarrier as well as a preparation method and application thereof. The cell culture microcarrier comprises a polymer porous microsphere with an inside-outside through pore channel structure. The cell culture micro-carrier comprises a biodegradable micro-carrier and also comprises a non-degradable micro-carrier, so that the growth of cells in the carrier is facilitated, and the mass transfer of nutrient substances and metabolites is also facilitated, thereby promoting the growth of the cells, enabling the cultured cells to keep good activity and function, and being high in proliferation efficiency; the method is suitable for three-dimensional culture of somatic cells, stem cells, tumor cells and the like.

The invention relates to a method for mechanically regulating and controlling a nerve cell calcium ion signal by using an Airy beam to control an optical bullet, which comprises the following steps: firstly preparing the optical bullet and culturing HT-22 cells, then dynamically regulating and controlling the cell calcium ion signal, and finally monitoring and recording an image in the regulation and control process in real time through a CMOS (Complementary Metal Oxide Semiconductor) camera. According to the method, non-contact mechanical stimulation on nerve cells can be realized. The spatial resolution limitation of the traditional nerve regulation and control technology is broken through, and a non-invasive and high-biocompatibility nerve signal regulation and control means is provided. Besides, in combination with a real-time fluorescence monitoring technology, the regulation and control effect can be intuitively evaluated, and a brand new tool is provided for neuroscience research and clinical application.

The present invention relates to a cell culture vessel characterized in that it comprises an inner surface on which one or more lectins suitable for binding to one or more species of microorganisms are immobilized. Furthermore, the invention refers to a method for cultivating cells comprising contacting the cell culture medium comprising the cells with a surface that bears immobilized lectin and to the use of immobilized lectin for reducing the microorganism contamination in a cell culture contacted with such surface.

The invention relates to a reactor for carrying out chemical or biological processes comprising a tank and at least one stirrer, wherein each stirrer comprises at least two propeller blades (5) which form a propeller (1), each propeller blade (5) being looped, wherein a tip (7) of a leading propeller blade (5) is curved back into its trailing propeller blade (5), into the hub or into the backside of the same propeller blade. The invention further relates to a process for cultivating cells or multicellular microorganisms using the reactor and a process for producing a stirrer.

The invention discloses a construction method and application of a mouse airway organoid inflammation model, and relates to the technical field of biology. The construction method comprises the following steps: (1) taking a mouse airway tissue, digesting with digestive juice, and centrifuging to obtain a cell suspension; (2) centrifuging the cell suspension, discarding the supernatant, adding matrigel, uniformly mixing, incubating, and culturing by using an airway organoid complete medium; (3) adding a cell recovery solution into the cells cultured in the step (2), incubating, degumming, centrifuging, discarding supernatant, adding matrigel, uniformly mixing, incubating, and adding an airway organ complete culture medium for continuous culture; and (4) adding 1-20 [mu] g / ml of LPS into the airway organoid complete culture medium, and incubating. The AO constructed by the invention covers four main cell types of cilia cells, goblet cells, basal cells and rod-like cells, causes epithelial injury in the AO and expression of AO inflammatory factors, and can provide reference for development of CRD new drugs.

To provide senolytic agents to eliminate senescent cells and methods for treating diseases and disorders associated with senescence.SOLUTION: Disclosed is a method for removing senescent cells from banked stem cells, the method comprising adding to the stem cells at least one senolytic agent. Also provided is a pharmaceutical composition comprising at least one senolytic agent for use in a method for treating a disease, disorder or condition associated with senescence in a subject, where the method comprises removing stem cells from the subject, culturing the cells with the pharmaceutical composition, and administering to the subject the cultured cells.SELECTED DRAWING: None

Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a transformed cell population. The methods include the steps of culturing a cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure, the hydrogel matrix also comprising a transforming agent.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention provides a gamma delta T cell preparation as well as a preparation method and application thereof, and belongs to the technical field of T cells. The liposome is prepared from the following raw materials in parts by weight: 15-20 parts of modified gamma delta T cell liposome, 1-3 parts of cell stimulating factors and 4-7 parts of culture medium freeze-dried powder, the modified gamma delta T cell lipidosome is prepared by embedding gamma delta T cells through lipidosome, coupling with acetylenic bond modified chitosan, and further mixing with MFAP4 protein and b4GALT1 protein. The culture medium freeze-dried powder is prepared by freeze-drying the culture medium in the engineering culture process of gamma delta T cells, and the cell stimulating factors are resveratrol and quercitrin. According to the gamma delta T cell preparation prepared by the invention, the survival ability and resistance of gamma delta T cells are improved, the anti-tumor activity of the gamma delta T cells is improved, and the gamma delta T cell preparation has relatively good antioxidant and anti-inflammatory effects, reduces the inhibition of inflammation on an immune system and has a very good treatment effect on pancreatic ductal adenocarcinoma.

The present invention provides methods for cryopreserving a population of cells with improved cell viability. In some aspects, the method comprises contacting a population of cells with a peptoid polymer comprising one or more polar peptoid monomers, e.g., formulated in a cryoprotectant solution, and cooling the population of cells at a temperature of from 0° C. to about −20° C. for a time period of at least about 3 hours to produce a population of supercooled cells. The supercooling methods of the present invention provide excellent post-thaw cell survival and recovery. In certain embodiments, the population of cells is present in a tissue or an organ that is cryopreserved by performing the supercooling methods of the present invention.

The invention relates to a method for rapidly detecting the cell adhesiveness of probiotics and application of the method. The method comprises the following steps: adding the probiotics into adherent culture cells, incubating, discarding liquid, digesting and collecting the cells by pancreatin, extracting total DNA (Deoxyribonucleic Acid), performing qPCR (Quantitative Polymerase Chain Reaction) amplification, and calculating the adhesion quantity of the probiotics on a single cell according to a Ct value. The invention provides a method for rapidly detecting the cell adhesion rate of probiotics by using a qPCR (quantitative polymerase chain reaction) technology, which comprises the following steps of: extracting total genome DNA (deoxyribonucleic acid) after the probiotics are adhered to cells on the basis of the gene expression difference of prokaryotic and eukaryotic cells, and detecting the specific gene expression level of the probiotics and the cells by using the qPCR technology. The cell adhesion ability of the probiotics is evaluated by calculating the ratio of the gene expression quantities of the two, and the method has the characteristics of high efficiency, rapidness, high accuracy and the like.

The invention relates to methods for culturing cells, and cultured animal cells for use in described method. Also provided is a modified animal cell and guide RNAs targeting genes to be modified in the modified and cultured cells of the invention.

The invention discloses a cell preparation treatment method, system and medium, and the method comprises the following steps: carrying out cell amplification treatment on a to-be-cultured cell sap in a cell culture container to obtain a cultured cell sap; washing and concentrating the cultured cell sap in the cell culture container to obtain concentrated cell sap; and sub-packaging the concentrated cell sap in the cell culture vessel into a plurality of preparation bags. According to the invention, cell culture, cell washing and concentrating treatment and preparation sub-packaging are realized on the cell culture container, so that scattered arrangement of equipment is avoided, and the occupied space is reduced; the steps are sequentially completed through the cell culture container, the whole process from cell culture to cell preparation can be ensured to be performed in a sealed sterile environment, cell sap does not need to be transferred, and the cell culture quality is improved; the steps are sequentially and integrally completed through the cell culture container, manual intervention is reduced, the whole process from cell culture to cell preparation is intelligently carried out, and the cell culture efficiency is improved.

The invention discloses automatic perfusion culture equipment for NK (Natural Killer) cell culture, which belongs to the technical field of cell culture equipment and comprises a liquid storage cylinder, a waste liquid cylinder, a mounting rack, a culture box, a first conveying mechanism, a second conveying mechanism, a driving mechanism and an anti-skid fixing mechanism. According to the automatic perfusion culture equipment for NK cell culture, through the arrangement of the first conveying mechanism, the second conveying mechanism and the driving mechanism, a nutrient solution in the liquid storage cylinder can be automatically conveyed into the culture box, a waste nutrient solution in the culture box can be automatically conveyed into the waste liquid cylinder, automatic perfusion culture of NK cells is achieved, and through flowing of the nutrient solution, the culture efficiency is improved. The mass transfer between nutrient substances and metabolic waste products and cells can be realized, so that the concentration accumulation of harmful metabolic waste is reduced, and the culture of NK cells is facilitated.

1. The name of the product of this design: bioreactor. 2. Purpose of the product of this design: bioreactor for culturing cells. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

This disclosure describes methods for growing cells on chickpea microcarriers as part of a process of creating a comestible food product. Generally, the disclosed method comprises adding chickpea microcarriers to cell culture media in a vessel. The chickpea microcarriers are mixed with non-human cells in the cell culture media. The chickpea microcarriers and non-human cells are mixed to adhere and grow the non-human cells on the surface of the chickpea microcarriers. After mixing, the non-human cells adhered to the surface of the chickpea microcarriers forms a textured cell tissue. The textured cell tissue can be harvested from the vessel and formed into a comestible food product.

The invention discloses a construction method, a recognition method and a system for simulating lung cancer metastasis cells in cerebrospinal fluid based on diffraction imaging and a deep learning technology. The construction method comprises the following steps: culturing a plurality of lung cancer cell lines which are easy to cause brain metastasis, collecting whole blood of a healthy person and splitting red blood cells; the method comprises the following steps: respectively acquiring diffraction images of cultured cells through a polarization diffraction imaging flow cytometry with a TDI camera to obtain a pair of diffraction image pairs with S polarization and P polarization; performing image processing on the obtained diffraction cell image pair, and establishing a training image data set and a test image data set; and establishing a convolutional neural network, and training the convolutional neural network according to the training image data set to obtain a model for identifying the lung cancer metastatic cells in the cerebrospinal fluid. When in use, a cerebrospinal fluid sample of a patient is collected, S polarization and P polarization image pairs are obtained, and the trained model is input to respectively extract S polarization and P polarization image features of cells for feature recognition, so that whether the current sample contains lung cancer cells or not is predicted.

The utility model relates to the technical field of cell culture, and discloses a cell culture bottle, comprising a culture bottle, wherein a bottom wall is arranged at the bottom of the culture bottle, a liquid-blocking and breathable membrane is arranged at the opening of the culture bottle, a closing cover is installed on the outside of the culture bottle, a fixing ring is fixedly connected to the outside of the culture bottle, a plug is arranged inside the fixing ring, and a sealing block is fixedly connected to the inside of the plug. The cell culture bottle has the advantages of being convenient for adding culture fluid and reducing the influence of the external environment on the internal environment of the culture bottle. The plug is used to close a pipeline, and the culture bottle is divided into two cavities by a partition plate, so that culture fluid can be added through the pipeline, and a blocking mechanism is opened by gravity to add culture fluid to the inside of the cavity for culturing cells, so as to complete the addition of culture fluid, thereby solving the problem that the external environment may contaminate cells when the closing cover is opened to pour the culture fluid through the opening during the current cell culture process.

The invention belongs to the technical field of biological culture, and particularly relates to a three-dimensional dynamic biological culture device and method. The three-dimensional dynamic biological culture device comprises: a culture chamber unit, which comprises a porous membrane and a chamber body with a first opening, the porous membrane and the chamber body are detachably enclosed to form a first chamber, and the first chamber can accommodate a curable culture medium for providing a growth space for culturing cells; the flow path plate unit comprises a base part, the base part is provided with a circulation groove and a liquid storage opening matched with a liquid storage device storing a culture solution, and the liquid storage opening is communicated with the circulation groove; a plurality of mounting ports which can be matched with the culture room units are formed in the circulating groove and are used for communicating the first chamber with the circulating groove through the porous membrane; and the driving unit and the circulating groove form a circulating loop so as to drive the culture solution in the circulating groove to flow in the plurality of culture room units. According to the invention, dynamic culture of three-dimensional cells, cell spheres and organoid cells can be better realized.

The invention relates to methods and apparatus for culturing cells. There is provided methods and apparatus for providing a seed train comprising a chemostat, turbidostat or auxostat and methods and apparatus for medium exchange during culturing using gravitational settling separation.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a vascularized organ chip and method for large-scale culture, the vascularized organ chip comprises an upper runner layer and a lower culture layer, and the runner layer and the culture layer are fixedly sealed; a liquid storage pipe matched with the flow channel layer is adhered to the upper surface of the flow channel layer; according to the vascularized organ chip, the flow channel layer is used for pouring a culture solution to provide fluid stimulation and nutrients for cells; the three parallel-connected large-scale culture chambers of the culture layer are used for culturing cells, so that blood circulation of a microvascular system in a human body can be simulated, and a microenvironment in tissues can be reproduced more truly; meanwhile, the requirement for culturing a large number of tissue cells through the chip can be met, and the cell yield and the experiment scale can be effectively increased.

The invention provides a preparation method and application of mesenchymal stem cell exosomes, and the method is characterized in that N-acetylcysteine and quercetin are added into a culture medium used for culturing MSC cells, so that the MSC cells are stimulated to generate exosomes with anti-aging and anti-apoptosis effects. In addition, the exosome generated by stimulating the MSC through a culture mode of gradually reducing oxygen partial pressure further has an anti-aging effect; the adverse effects of acute oxidative stress, decline of multiplication capacity, unstable quality and the like in a low-oxygen state can be inhibited, and the activity of related components of cells and exosomes is not damaged. The method shows good anti-aging and anti-apoptosis effects in vitro, and a new thought is provided for treating aging diseases by the exosome in the future.

The invention provides an applicable population screening method and application of a glioma dry-like cell sensitized DC vaccine, peripheral blood of a patient before and after inoculation of the glioma dry-like cell sensitized DC vaccine is obtained, mononuclear cells are extracted, CD8 + T cells are respectively separated from the mononuclear cells through a magnetic bead sorting method, and the CD8 + T cells are separated from the mononuclear cells. The method comprises the following steps: uniformly inoculating CD8 < + > T cells into an ELISPOT cell culture plate coated with an IFN-gamma antibody for culture, respectively setting an experimental group and a positive control group for culture, adding A2B5 < + > glioma cell lysis buffer into the experimental group and adding phytohemagglutinin into the positive control group, and carrying out ELISPOT staining on the cultured cells to obtain the secretion amount of FN-gamma before and after treatment of a patient. According to the secretion amount of the FN-gamma, patients with specific immune response to the glioma dry-like cell sensitization DC vaccine are screened, so that the glioma dry-like cell sensitization DC vaccine is conveniently popularized and applied, and the immune mechanism of the glioma dry-like cell sensitization DC vaccine is researched.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a function-enhanced mesenchymal stem cell exosome, the preparation method comprises the following steps: S1, preparing an NK cell exosome, collecting supernate in an NK cell culture medium, and treating the supernate by using an optimized exosome separation and purification process to obtain an NK cell exosome; finishing preparation of a stock solution; s2, culturing the mesenchymal stem cells, culturing the cells in a 3D suspension culture mode, treating and controlling low oxygen concentration by combining with NK cell exosomes, and simulating an in-vivo inflammation microenvironment in a multi-azimuth manner, so that the exosomes secreted by the mesenchymal stem cells have a stronger immune regulation function and an anti-inflammatory effect; s3, preparation of mesenchymal stem cell exosomes: preparing a stock solution through an exosome separation and purification process combining a tangential flow ultrafiltration technology and a PS affinity chromatography technology; the exosome stock solution obtained by the method is high in purity and high in yield, and has more excellent biological activity and excellent treatment effect.

This disclosure describes methods for improving umami and meaty flavors of cell-based food products. In particular, the disclosed method includes an amino acid integration method where cultured cells and exogenous amino acids (e.g., glutamic acid and aspartic acid) are combined and heated, resulting in a synergistic improvement of umami flavors. The disclosed method further includes a browning method by which a cell suspension is dehydrated and heated at high temperatures (e.g., greater than 140C) to induce non-enzymatic browning and caramelization reactions in the cell suspension. The cell-based flavoring product can be combined with a food product as a flavor, aroma, and color enhancer. The disclosed methods may use one or both of the amino acid integration method and the browning method to improve organoleptic properties of cell-based food products.