54 results about "Mevalonic acid" patented technology

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for preparing stevioside through de novo fermentation as well as a preparation method and application of the engineering bacterium. According to the invention, mevalonate kinase in the MVA pathway is mutated and optimized. The method comprises the following steps: carrying out point mutation on mevalonate kinase MvK of a wild type source, constructing a stevioside synthesis route in an escherichia coli host, and comparing the influence of MvK mutation on the yield of stevioside, so as to determine a Q160L mutant, and applying the Q160L mutant to the stevioside production route to realize efficient production of stevioside.

The invention discloses a recombinant bacterium capable of increasing the yield of alpha-bisabolol as well as a preparation method and application of the recombinant bacterium, and belongs to the technical field of microorganisms. The invention aims to improve the yield of alpha-bisabolol and enhance the tolerance of a host to an organic solvent. The invention provides a recombinant bacterium for improving the yield of alpha-bisabolol. Escherichia coli is used as a starting strain; the method comprises the following steps of: overexpressing an acetyl CoA acyltransferase / HMG-CoA reductase mvaE gene, an HMG-CoA synthetase mvaS gene, a 2-methyl citrate dehydratase prpD gene, a mevalonate kinase ERG12 gene, a mevalonate 5-phosphate kinase ERG8 gene, a mevalonate 5-diphosphate decarboxylase ERG19 gene and an isopentenyl diphosphate isomerase idi gene, so as to obtain a recombinant vector; the gene is obtained from an alpha-bisabolol synthase gene of artichoke, a farnesyl diphosphate synthase ispA gene and an alpha-bisabolol synthase CcBOS gene of artichoke. The industrial process of synthesizing alpha-bisabolol by a biological method is promoted.

The invention is related to a process for synthesizing a sustainable aviation fuel composition. The process comprises providing a reaction mixture comprising a first compound that is at least one of mevalonolactone, mevalonic acid, mevalonate salt, dehydromevalonic acid, dehydromevalonate salt, dehydromevalonolactone or combinations thereof. The process then involves converting the first compound in the reaction mixture to provide a first intermediate comprising isoprene. Then, the isoprene in the first intermediate is reacted in the presence of a first heat transfer agent to provide a second intermediate comprising terpenes. Finally, the second intermediate is allowed to react in the presence of a second heat transfer agent, or alternatively in neat conditions, and optionally in presence of a catalyst to provide the sustainable fuel composition. The sustainable aviation fuel composition made available from the process of the invention is found to comprise monocyclic aromatic hydrocarbons (MAHs) at useful concentration ranges (along with cycloalkanes) while they are substantially devoid of Polycyclic aromatic hydrocarbons (PAHs).

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing zeaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, the yarrowia lipolytica is subjected to genetic engineering modification based on a genetic engineering technology, a zeaxanthin biosynthetic pathway is constructed by expressing beta-carotene hydroxylase in an original strain, and meanwhile, a zeaxanthin biosynthetic pathway is constructed through a multiple metabolic engineering modification strategy. The method comprises the steps of key enzyme PaCrtZ multi-copy integration, oxidation-reduction balance regulation and control, upstream mevalonic acid pathway enhancement through membrane-free organelles and the like, and the yield of zeaxanthin is further increased. Meanwhile, by optimizing the culture conditions and applying the optimal fermentation conditions to perform tank fermentation, the yarrowia lipolytica can continuously generate more zeaxanthin, so that the yarrowia lipolytica has excellent practical application value and application prospect.

The invention discloses a recombinant bacterium for producing mevalonic acid by taking glucose and acetone as co-substrates as well as a construction method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. The problems that an existing mevalonic acid biosynthesis mode is low in carbon atom economy, limited in theoretical yield and the like are solved. According to the invention, acetone carboxylase, acetoacetyl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase and hydroxymethyl glutaryl-CoA synthetase are subjected to heterologous expression in escherichia coli with an acetyl coenzyme A acetyltransferase gene atoB and a histidine protein kinase gene atoS knocked out, and self carbonic anhydrase is over-expressed; and a new way for synthesizing MVA by using glucose and acetone as a co-substrate is constructed. According to the method, one molecule of CO2 can be fixed in the biosynthesis of mevalonic acid, the yield of carbon atoms in the synthesis process of mevalonic acid is increased, the problem of low yield of carbon atoms in the synthesis process of mevalonic acid by taking glucose as a single substrate is solved, and a new thought is provided for constructing a carbon neutralization type biological manufacturing technology.

The present application relates to the promotion oil bean root secretion of mevalonic acid microbial source humic acid fertilizer, the promotion oil bean root secretion of mevalonic acid artificial synthesis microbial source humic acid is prepared by the following method: the corn stalk is predecomposed by using the big ball cover mushroom strain, and the corn slurry with the concentration of 0-1% is added, the moisture content of corn stalk is 55-65%, the predecomposed corn stalk is added with water, is handled with water treatment device, and the mixture containing microbial fungal residues is obtained;The mixture containing microbial fungal residues is converted into artificial synthesis microbial source humic acid by four-stage reaction;The artificial synthesis microbial source humic acid fertilizer is applied to the root of oil bean, the mevalonic acid secreted by the root of oil bean reaches 0.006-0.007 ng / mg, and the length of the root of oil bean reaches 25-26 cm.The present application can improve the content of mevalonic acid secreted by the root of oil bean, improve the yield and quality of oil bean.

This invention belongs to the field of microbial metabolic engineering and biotechnology applications, specifically relating to an engineered bacterium for synthesizing monoterpenoid compounds, its construction method, and its applications. The construction method involves using *Hansenula polymorpha* as a host, regulating the mevalonate pathway in its cytoplasm, and overexpressing monoterpene synthase to obtain the engineered bacterium; or, overexpressing monoterpene synthase and the mevalonate pathway in peroxisomes to obtain the engineered bacterium. The engineered bacterium constructed in this application can efficiently synthesize monoterpenoid compounds such as geraniol, β-myrcene, and dextrorotatory borneol.

The invention provides application of a group of plasma metabolism markers in preparation of a reagent product for pulmonary nodule diagnosis, and relates to the technical field of disease diagnosis reagents. The metabolic marker is prepared from trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin, and the metabolic marker is prepared from the following raw materials: the trigonelline, the 4-methylcatechol, the 2, 4-dinitrophenol, the 3, 4-dihydroxyphenylacetic acid, the N-( According to the application, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC / MS) is adopted to carry out metabonomics analysis on plasma samples before and after an early pulmonary nodule operation, and a group of plasma metabolites (trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin) are identified; and the group of metabolites is utilized to form a diagnosis model, so that benign and malignant pulmonary nodules can be accurately diagnosed, and early-stage differential diagnosis and risk assessment are facilitated.

The invention provides a strain for producing beta-carotene as well as a construction method and application of the strain, and belongs to the technical field of synthetic biology and genetic engineering. The construction method of the MYy107 strain disclosed by the invention comprises the following steps: step 1, integrating a mevalonate kinase gene ERG12, a phosphomevalonate kinase gene ERG8, a mevalonate diphosphate decarboxylase gene ERG19 and an isopentene diphosphate isomerase gene IDI1 on an RT08 bacterium, so as to obtain an RT10 strain; 2, a xylose reductase gene XYL1, a xylitol dehydrogenase gene XYL2, a xylose isomerase mutant XylA3 * and a xylulokinase gene Yl.Xk are integrated on the RT10 strain, and the MYy107 strain is obtained. According to the MYy107 strain constructed by the invention, the beta-carotene can be produced by using xylose and acetic acid at the same time, and the yield of the beta-carotene is remarkably improved and reaches 710.01 mg / L.

The invention relates to the technical field of microorganisms, and discloses recombinant yeast for efficiently synthesizing a GPP derivative compound as well as a construction method and application of the recombinant yeast. The recombinant yeast strain takes a yeast strain as an original strain, and is obtained by introducing a target coding gene into a cell nucleus; the target coding gene comprises a coding gene of a mevalonic acid (MVA) synthetic pathway enzyme, a coding gene of a farnesyl diphosphate synthase mutant and a coding gene of optional geranyl diphosphate derivative synthase. According to the invention, an MVA pathway and a key enzyme are introduced into a cell nucleus, so that competitive consumption of a sterol synthesis pathway in cytoplasm on geranyl diphosphate (GPP) is effectively avoided, and efficient synthesis of the GPP derivative compound is realized. Besides, the recombinant yeast strain disclosed by the invention can be combined with a reported peroxisome platform to construct a double-organelle platform strain, so that the yield of GPP derivative compounds can be further increased.

The invention is related to a process for synthesizing a sustainable aviation fuel composition. The process comprises providing a reaction mixture comprising a first compound that is at least one of mevalonolactone, mevalonic acid, mevalonate salt, dehydromevalonic acid, dehydromevalonate salt, dehydromevalonolactone or combinations thereof. The process then involves converting the first compound in the reaction mixture to provide a first intermediate comprising isoprene. Then, the isoprene in the first intermediate is reacted in the presence of a first heat transfer agent to provide a second intermediate comprising terpenes. Finally, the second intermediate is allowed to react in the presence of a second heat transfer agent, or alternatively in neat conditions, and optionally in presence of a catalyst to provide the sustainable fuel composition. The sustainable aviation fuel composition made available from the process of the invention is found to comprise monocyclic aromatic hydrocarbons (MAHs) at useful concentration ranges (along with cycloalkanes) while they are substantially devoid of Polycyclic aromatic hydrocarbons (PAHs).

A strain of Yarrowia lipolytica was engineered to overexpress β-hydroxy β-methylglutaryl-CoA reductase and farnesyl pyrophosphate synthase, as rate-limiting enzymes in the mevalonate and sesquiterpenoid synthesis pathways respectively. Metabolite extracts from this strain were run on LC-MS and showed a number of novel compounds being produced, including terpenoids varying in lengths and oxidation states. Upon NMR and MS / MS structure validation as well as biochemical assays, these compounds were determined as a new class of non-natural compounds, bifunctional terpenoids. Studies on the overexpression of P450 enzymes, alcohol oxidase, aldehyde dehydrogenase, and alcohol dehydrogenase showed that expression of these enzymes in addition to β-hydroxy β-methylglutaryl-CoA reductase and farnesyl pyrophosphate synthase increase the production of bifunctional terpenoids. Bioactivity assays demonstrate the application of bifunctional terpenoids.

The invention discloses a method for preparing a laying hen feed additive by fermenting tea-seed oil deodorized distillate, which is characterized in that the tea-seed oil deodorized distillate is used as a raw material, and efficient utilization of all components of resources is achieved by coupling yeast-paecilomyces cicadae two-step sequential fermentation and endogenous mevalonic acid (MVA) pathway cascade regulation and control technology. The final product is rich in ergosterol, vitamin E and probiotics, can improve the laying rate of laying hens and enhance the eggshell strength after being applied to laying hen breeding, realizes zero wastewater discharge in the process, and achieves the dual goals of resource utilization of grease byproducts and accurate matching of laying hen nutrition.

The invention discloses a LiHMGR gene for regulating and controlling biosynthesis of lilium steroid saponin substances and application of the LiHMGR gene, and belongs to the technical field of molecular biology. The invention finds that the spatio-temporal expression trend of the LiHMGR gene in the lily bulb development period is first reduced, then increased and then reduced, which is consistent with the overall change trend of synthesis of steroid saponin substances; furthermore, VIGS determines that silence of LiHMGR can significantly inhibit synthesis of mevalonic acid, and overexpression of LiHMGR can significantly increase the content of mevalonic acid. Experimental results show that the LiHMGR protein plays a positive regulation role in synthesis of steroid saponin of the lily'orange sunlight 'bulb.

The invention discloses kluyveromyces marxianus genetically engineered bacteria and application thereof, and belongs to the technical field of bioengineering. The invention discloses a kluyveromyces marxianus genetically engineered bacterium. The kluyveromyces marxianus genetically engineered bacterium is prepared by the following steps: firstly, screening to obtain a kluyveromyces marxianus strain with high ergosterol yield; on the basis, inducing and separating a MATa haploid strain; then, through the introduction of heterologous DHCR24 and DWF5 genes, the weakening of a competitive pathway gene ERG6, the improvement of the metabolic flux of a squalene pathway in a sterol synthesis process, the compartment engineering of a peroxisome region of a mevalonic acid pathway, the knockout of an hypoxia inhibition transcription factor ROX1, the improvement of cofactor supply, the optimization of a yeast lipid pathway and other metabolic engineering optimization reconstructions, the expression of the yeast lipid pathway is improved; and an engineering strain with high cholesterol yield is constructed and obtained. Through fed-batch fermentation, efficient and stable synthesis of cholesterol is achieved, the yield reaches 7.64 g / L, and a foundation is laid for biological manufacturing of cholesterol.

The invention belongs to the technical field of microbial fermentation. The invention relates to saccharomyces cerevisiae, in particular to saccharomyces cerevisiae with high yield of farnesene and squalene and application of the saccharomyces cerevisiae. The yeast-like yeast is constructed by the following steps: providing yeast-like host bacteria; an exogenous farnesene synthase coding gene is introduced into the yeast-like host bacteria, so that the farnesene synthase coding gene is expressed in a host; transforming a genome of the yeast-like host bacterium so as to improve the expression activity of endogenous squalene synthase of the yeast-like host bacterium; strengthening the mevalonic acid pathway of the yeast-like host bacteria; and screening to obtain an engineering strain capable of simultaneously accumulating farnesene and squalene, thereby obtaining the saccharomyces-like strain capable of producing farnesene and squalene at high yield. The genetic stability of the saccharomyces cerevisiae meets the condition that more than 90% of farnesene and squalene producing capacity is still maintained after continuous passage for 50 generations in a culture medium without selective pressure.

The invention relates to a genetically engineered bacterium for producing (-)-limonene, (-)-carvol and (-)-carvone and application of the genetically engineered bacterium. According to the method, endogenous ATP-citrate lyase genes ACL and CarYB genes are knocked out, endogenous mevalonic acid pathway key genes HMGR, ACCT and IDI are overexpressed, a mevalonic acid kinase gene MmMK sourced from methanosarcina martensii and mevalonic acid pathway key genes EfMvaS and EfMvaE sourced from enterococcus faecalis are introduced, and the (-)-limonene, (-)-carvol and (-)-carvone are synthesized into the genetically engineered strain. The method is realized in a platform strain for regulating and controlling the balance of the cofactor NADPHP. And the yield of the (-)-limonene in a bioreactor of 500-mL reaches 608.5 mg / L. The yield of the (-)-carveol reaches 667.6 mg / L when the (-)-carveol is fermented in a 500-mL bioreactor. The fermentation yield of the recombinant strain for producing the (-)-carvone in a 500-mL bioreactor reaches 67.1 mg / L.

The application discloses a genetically engineered bacterium for improving the yield of mevalonic acid as well as a construction method and application thereof, and belongs to the technical field of genetic engineering. In order to solve the technical problems of low carbon atom economy and limited theoretical yield of MVA biosynthesis technology, the application takes Escherichia coli as a starting strain, overexpresses a bifunctional acetaldehyde-ethanol dehydrogenase mutant AdhE A267T / E568K gene adhE mut , an acetyl-CoA acyltransferase / HMG-CoA reductase gene mvaE , an HMG-CoA synthetase gene mvaS , and introduces a tetracycline-induced CRISPRi system to inhibit the expression of an orotate-5'-phosphate decarboxylase gene pyrF , so that the carbon flux is led from the growth of the strain to the synthesis of mevalonic acid. The genetically engineered bacterium provided by the application can produce mevalonic acid by fermentation with ethanol as a carbon source, and the yield reaches 0.89 g / g. The application provides a new idea for improving the carbon atom economy of mevalonic acid biological manufacturing.

The invention discloses an engineering strain for biosynthesizing gibberellin A3 from beginning by utilizing saccharomyces cerevisiae and a construction method of the engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a gibberellin A3 synthesis pathway is constructed in the saccharomyces cerevisiae, and the endogenous mevalonic acid biosynthesis pathway is optimized, so that the supply of GGPP is promoted, the yield of gibberellin A3 is further improved, and the yield of gibberellin A3 is up to 15.7 mg / L through shake-flask fermentation of the constructed engineering strain. The strain has good recovery stability and passage stability, and lays an important foundation for industrial biosynthesis of gibberellin compounds.

Disclosed are inhibitors of mevalonate pathway as an efficient vaccine adjuvant and use thereof. In particular, the inhibitor is an acetoacetyl-CoA transferase inhibitor, a HMG-COA synthase inhibitor, a HMG-CoA reductase inhibitor, a mevalonate kinase inhibitor, a phosphomevalonate kinase inhibitor, a mevalonate-5-pyrophosphate decarboxylase inhibitor, an isopentenyl pyrophosphate isomerase inhibitor, a farnesyl pyrophosphate synthase inhibitor, a geranylgeranyl pyrophosphate synthase inhibitor or a geranylgeranyl transferase (I, II) inhibitor. Also disclosed is an immunogenic composition including inhibitors of mevalonate pathway as an adjutant.

The invention discloses yarrowia lipolytica genetically engineered bacteria capable of producing decaisopentenol and application of the yarrowia lipolytica genetically engineered bacteria, and belongs to the technical field of bioengineering. The yarrowia lipolytica genetically engineered bacterium is obtained by cloning a decene diphosphate synthase gene into yarrowia lipolytica, overexpressing related genes of an endogenous acetyl coenzyme A synthetic pathway and a mevalonic acid pathway of the yarrowia lipolytica, weakening a squalene synthetic pathway and knocking out endogenous ylCOQ1 enzyme. Experiments prove that the yarrowia lipolytica genetically engineered bacterium can significantly improve the yield of decyl isopentenol, and the product serves as an important precursor for chemical synthesis of coenzyme Q10, so that the engineered bacterium and the product thereof can be applied to biochemical synthesis of coenzyme Q10. The method is green and efficient, and has important industrial application value.

The present invention provides a use of mevalonic acid in the manufacture of a drug for improving early embryonic development of aged oocytes, which belongs to the field of biomedicine technology. Through experimental research, the present invention has found that mevalonic acid can be used to improve the fertilization rate, two-cell formation rate and good-quality blastocyst formation rate of aged oocytes, thereby improving the development process of early embryos, providing a new idea and method for infertility treatment.

The invention discloses saccharomyces cerevisiae with high yield of mevalonic acid and application of the saccharomyces cerevisiae in synthesis of terpenoids. According to the invention, a strain of saccharomyces cerevisiae sSca5 with strong natural mevalonic acid synthesis capability is obtained, and the preservation number is GDMCC No: 67359. According to the invention, saccharomyces cerevisiae sSca5 is taken as a chassis cell, the engineering strain S7 with high yield of mevalonic acid is successfully constructed through multilevel metabolic engineering transformation, and the yield of mevalonic acid reaches 14.71 g / L. Further, the alpha-bergamotene synthase and the alpha-farnesene synthase are respectively introduced into the engineering strain S7, and the yield of the alpha-bergamotene and the yield of the alpha-farnesene respectively reach 1.97 g / L and 3.67 g / L. The strain platform provided by the invention is not only suitable for efficient production of mevalonic acid, but also can be used as a general chassis for biosynthesis of various terpenoids, and has a wide industrial application prospect in the fields of medicines, spices, cosmetics and the like.

The invention discloses a mevalonate pyrophosphate decarboxylase mutant MVD1m and application thereof in synthesis of squalene, the amino acid sequence of the mevalonate pyrophosphate decarboxylase mutant MVD1m is based on a mevalonate pyrophosphate decarboxylase crystal structure, and the amino acid sequence of the mevalonate pyrophosphate decarboxylase mutant MVD1m is obtained through rational design. The mutant sequence (MVD1m) with improved affinity with the substrate mevalonate pyrophosphate is obtained, and the mutant sequence (MVD1m) has (1) an amino acid sequence as shown in SEQ ID NO.1. The mevalonate pyrophosphate decarboxylase mutant MVD1m disclosed by the invention has relatively high binding force with a substrate mevalonate pyrophosphate, and is applied to the synthesis of squalene, so that the synthesis efficiency of the squalene is effectively improved.

The invention provides a pramoxine hydrochloride and fluconazole combined pharmaceutical composition for resisting candida albicans biofilm infection and application of the pharmaceutical composition, and belongs to the technical field of biological medicine. Aiming at the problem of inherent drug resistance of a candida albicans biological membrane to azole drugs, drug relocation strategy screening finds that pramoxine hydrochloride can significantly enhance the inhibitory activity of fluconazole to the candida albicans biological membrane. The action mechanism is as follows: pramoxapine hydrochloride is specifically targeted to key protein mevalonate kinase in a farnesyl pyrophosphate synthesis route in candida albicans, biosynthesis of ubiquinone is inhibited, the active oxygen level of mitochondria is increased, the mitochondrial function is destroyed, the hypha length, the biological membrane thickness and extracellular matrix secretion of candida albicans are reduced, and the activity of pramoxapine hydrochloride in the candida albicans is improved. And finally, the anti-biofilm effect of fluconazole is improved. The invention provides a safe and efficient new strategy for resisting Candida albicans biofilm infection, and provides a new pharmaceutical composition and an action target for clinical treatment of biofilm related fungal infection.

The application provides a saccharomyces cerevisiae engineering bacterium for producing squalene through endogenous and artificial synthetic pathways and an application thereof, the saccharomyces cerevisiae engineering bacterium takes ZS00 strain as a starting strain, overexpresses acetyl coenzyme A acetyltransferase gene, hydroxymethylglutaryl coenzyme A synthase gene and N-terminal truncated hydroxymethylglutaryl coenzyme A reductase gene, and effectively improves the yield of squalene. Heterologous expression of mevalonate kinase gene and mevalonate pyrophosphate decarboxylase gene, overexpression of the key gene squalene synthase gene for promoting the conversion of precursors into squalene effectively promotes the synthesis of squalene. The introduction of isopentenol utilization pathway genes and their mutants, through the regulation of IU pathway key substrate, overexpression of isopentenyl pyrophosphate isomerase gene and farnesyl pyrophosphate synthase gene, the final strain can effectively accumulate squalene to 687.93 mg / L. The application realizes the breakthrough of squalene yield and efficiency through complementation and synergistic effect.

The present application relates to a kind of rapid detection method of multiple target protein of engineering strain methylmalonic acid (MVA) pathway based on multiple reaction monitoring technology (MRM).Firstly, the MRM acquisition method is established with the target peptide segment;The target peptide segment standard is prepared into at least 5 different concentrations, and data is collected using liquid chromatography-mass spectrometry technology and MRM method, with concentration value and peak area as horizontal and vertical coordinates to draw standard curve;Engineering strain sample is obtained by using conventional proteomics sample pretreatment process Enzymolysis peptide segment solution, mass spectrometry data is collected using liquid chromatography-mass spectrometry technology and MRM method, and then external standard method is used for protein quantification.The present application is based on traditional external standard method quantification, constructs target protein detection method based on short chromatographic column, microliter liquid chromatography system, short separation gradient and MRM quantification strategy, and it is used for the rapid quantitative analysis of the five proteins of engineering strain MVA pathway.

This invention relates to a recombinant photosynthetic bacterial strain with high tocopherol production, its construction method, and its applications. The recombinant strain knocks out the photosynthetic gene repressor transcription factor PpsR and integrates the mevalonate MEV pathway gene from *Paragonimococcus zeatans*, the p-hydroxyphenylpyruvate dioxygenase (HPPD) gene from *Pseudomonas putida*, the urokinase chlorophyllase (HPT) gene from *Synostemma pentaphyllum*, the tocopherol cyclase (TC) gene from *Arabidopsis thaliana*, and feedback-resistant 3-deoxy-D-arabinohepenoyl-7-phosphate synthase (AroG*). D146N Genes and prephenyl acid dehydrogenase TyrA* M53I Genes. This recombinant photosynthetic bacterium strain achieves highly efficient heterologous synthesis of tocopherol by knocking out regulatory genes and integrating multiple metabolic modules, with a fermentation yield of up to 3.51 g / L, showing broad prospects for industrial application.

The invention relates to a photosynthetic bacterium recombinant strain with high yield of tocopherol as well as a construction method and application of the photosynthetic bacterium recombinant strain. The photosynthetic bacterium recombinant strain knocks out a photosynthetic gene inhibition transcription factor PpsR of photosynthetic bacteria; a mevalonic acid MEV pathway gene for producing paracoccus zeatin, a p-hydroxyphenylpyruvate dioxidase HPPD gene from pseudomonas putida, a uromelanic acid chlorophyllin transferase HPT gene from synechocystis, a tocopherol cyclase TC gene from arabidopsis thaliana, a recombinant plasmid and a recombinant plasmid are integrated; the invention also discloses a 3-deoxy-D-arabinoheptulose-7-phosphate synthase AroG * D146N gene resistant to feedback and a pre-benzoate dehydrogenase TyrA * M53I gene resistant to feedback. According to the photosynthetic bacterium recombinant strain, efficient heterologous synthesis of tocopherol is achieved by knocking out regulation genes and integrating multi-way metabolism modules, the fermentation yield can reach 3.51 g / L, and the photosynthetic bacterium recombinant strain has wide industrial application prospects.

The present disclosure provides a structurally related family of pro-gelling decarboxylase homologues for use in commercial foods and cosmetics. The GPDH protein is expressed in cells in plants, animals and eukaryotic microorganisms in a trace form, and plays a catalytic role in a mevalonic acid pathway. Members of the GPDH family are structurally associated with one another through a series of strictly conserved amino acid sequence motifs. The ability to promote gelation when GPDH is used as a commercial product ingredient has been unclear yet before. The low gelation onset temperature (about 50 DEG C) and very low critical gelation concentration (4%) make the GPDH proteins of the present disclosure particularly suitable as functional protein substitutes in food and personal care products compared to most commonly used plant proteins and plant protein isolates.