Biofuel and chemical production by recombinant microorganisms via fermentation of proteinacious biomass

A technology for recombining microorganisms and biomass, applied in biofuels, biochemical equipment and methods, fermentation, etc., can solve problems such as storage and distribution systems

Inactive Publication Date: 2013-09-18
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ethanol is also hygroscopic and corrosive, c...

Method used

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  • Biofuel and chemical production by recombinant microorganisms via fermentation of proteinacious biomass
  • Biofuel and chemical production by recombinant microorganisms via fermentation of proteinacious biomass
  • Biofuel and chemical production by recombinant microorganisms via fermentation of proteinacious biomass

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Embodiment

[0186] Escherichia coli was chosen as the engineered host organism because of the versatility shown by this organism. To test the efficiency of algal amino acid utilization by E. coli, the indicated cells were grown on yeast extract or a mixture of 20 amino acids (used to mimic algal extract). E. coli grew well on these enriched media, as expected. However, amino acid utilization is incomplete, possibly due to amino acid imbalance and lack of branched-chain amino acid (BCAA) degradation pathways. Wild-type E. coli will each utilize only four of the amino acids (Ala, Asp, Pro, and Gln) as a single carbon source to form colonies. When the isobutanol synthesis pathway (overexpression of alsS, ilvC, ilvD, KivD and AdhA genes) was introduced into E. coli, the cells could produce only 0.17 g / L isobutanol (Table 4 below), representing a theoretical yield of 2.3%.

[0187] Table 4: Production of higher alcohols (C≥4) in Escherichia coli in yeast extract medium containing 21.6 g / L a...

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Abstract

Provided herein are metabolically modified microorganisms characterized by having an increased keto-acid flux when compared with the wild-type organism and comprising at least one polynucleotide encoding an enzyme, and causing the production of a greater quantity of a chemical product when compared with the wild-type organism. The recombinant microorganisms are useful for producing a large number of chemical compositions from various nitrogen containing biomass compositions and other carbon sources. More specifically, provided herein are methods of producing alcohols, acetaldehyde, acetate, isobutyraldehyde, isobutyric acid, n- butyraldehyde, n-butyric acid, 2-methyl-l-butyraldehyde, 2-methyl-l -butyric acid, 3- methyl-l-butyraldehyde, 3 -methyl- 1 -butyric acid, ammonia, ammonium, amino acids, 2,3-butanediol, 1,4-butanediol, 2-methyl-l, 4-butanediol, 2-methyl- 1,4-butanediamine, isobutene, itaconate, acetoin, acetone, isobutene, 1,5-diaminopentane, L-lactic acid, D- lactic acid, shikimic acid, mevalonate, polyhydroxybutyrate (PHB), isoprenoids, fatty acids, homoalanine, 4-aminobutyric acid (GABA), succinic acid, malic acid, citric acid, adipic acid, p-hydroxy-cinnamic acid, tetrahydrofuran, 3-methyl-tetrahydrofuran, gamma-butyrolactone, pyrrolidinone, n-methylpyrrolidone, aspartic acid, lysine, cadeverine, 2-ketoadipic acid, and/or S-adenosyl-methionine (SAM), from a suitable nitrogen rich biomass.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 61 / 409,760, filed November 3, 2010, and U.S. Provisional Patent Application Serial No. 61 / 410,174, filed November 4, 2010, the disclosures of which are incorporated by reference in their entirety incorporated into this article. [0003] governmental support [0004] This invention was made with Government support under Grant No. DE-FC02-02ER63421 awarded by the US Department of Energy. The government has certain rights in this invention. technical field [0005] Metabolically modified microorganisms and methods of producing such microorganisms are provided. The present invention provides a biofuel reactor system for generating biofuel from proteinaceous biomass. Also provided are methods of producing biofuels and chemicals by contacting suitable substrates with metabolically modified or recombinant microorganisms and enzyme preparation...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N1/19C12N1/15C12N15/63C12N15/52C12P7/00C12P13/00
CPCC12N1/20C12N9/0016C12N9/1096C12P7/04C12P7/24C12P7/40C12P13/04C12P7/16Y02E50/10Y02P20/582C12N15/63C12N15/75C12N15/77C12N15/78C12N15/80C12N15/81C12N15/815
Inventor J·C·廖赵光命闫亚军霍毅欣
Owner RGT UNIV OF CALIFORNIA
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