Biofuel and chemical production by recombinant microorganisms via fermentation of proteinacious biomass

Abstract

Provided herein are metabolically modified microorganisms characterized by having an increased keto-acid flux when compared with the wild-type organism and comprising at least one polynucleotide encoding an enzyme, and causing the production of a greater quantity of a chemical product when compared with the wild-type organism. The recombinant microorganisms are useful for producing a large number of chemical compositions from various nitrogen containing biomass compositions and other carbon sources. More specifically, provided herein are methods of producing alcohols, acetaldehyde, acetate, isobutyraldehyde, isobutyric acid, n- butyraldehyde, n-butyric acid, 2-methyl-l-butyraldehyde, 2-methyl-l -butyric acid, 3- methyl-l-butyraldehyde, 3 -methyl- 1 -butyric acid, ammonia, ammonium, amino acids, 2,3-butanediol, 1,4-butanediol, 2-methyl-l, 4-butanediol, 2-methyl- 1,4-butanediamine, isobutene, itaconate, acetoin, acetone, isobutene, 1,5-diaminopentane, L-lactic acid, D- lactic acid, shikimic acid, mevalonate, polyhydroxybutyrate (PHB), isoprenoids, fatty acids, homoalanine, 4-aminobutyric acid (GABA), succinic acid, malic acid, citric acid, adipic acid, p-hydroxy-cinnamic acid, tetrahydrofuran, 3-methyl-tetrahydrofuran, gamma-butyrolactone, pyrrolidinone, n-methylpyrrolidone, aspartic acid, lysine, cadeverine, 2-ketoadipic acid, and / or S-adenosyl-methionine (SAM), from a suitable nitrogen rich biomass.

Description

[0001] Cross References to Related Applications

[0002] This application claims priority to U.S. Provisional Patent Application Serial No. 61 / 409,760, filed November 3, 2010, and U.S. Provisional Patent Application Serial No. 61 / 410,174, filed November 4, 2010, the disclosures of which are incorporated by reference in their entirety incorporated into this article.

[0003] governmental support

[0004] This invention was made with Government support under Grant No. DE-FC02-02ER63421 awarded by the US Department of Energy. The government has certain rights in this invention. technical field

[0005] Metabolically modified microorganisms and methods of producing such microorganisms are provided. The present invention provides a biofuel reactor system for generating biofuel from proteinaceous biomass. Also provided are methods of producing biofuels and chemicals by contacting suitable substrates with metabolically modified or recombinant microorganisms and enzyme preparation...

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