25 results about "Isobutanol" patented technology

There is provided a method for producing isobutylene, in which isobutylene is produced from isobutanol with a high selectivity while suppressing a decrease in the isobutanol conversion rate under pressure. In the method for producing isobutylene according to the present invention, a raw material gas containing isobutanol is brought into contact with a catalyst to produce isobutylene from isobutanol, the method including bringing the raw material gas containing isobutanol into contact with a catalyst at a linear velocity of 1.20 cm / s or more under a pressure of 120 kPa or more in terms of absolute pressure to produce isobutylene from isobutanol.

The invention discloses a catalyst for acetylene method synthesis of vinyl acetate, a preparation method and application thereof, and relates to the technical field of acetylene gas phase method synthesis of vinyl acetate, in the preparation process of the catalyst, a mixed solvent is used for dissolving an active component, then a carrier is impregnated, the volume of water in the mixed solvent is 20-95%, and the volume of water in the carrier is 20-95%; the residual volume is one or a mixture of two of methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, or a mixture of three or more than three solvents. The solvent can significantly reduce the polarity of the solution, and experiments and characterization show that the agglomeration of the active components is significantly reduced, the dispersion of the active components is enhanced, and the performance of the catalyst is improved.

The application provides a preparation method of dibutyl adipate, belongs to the technical field of organic compound preparation, and can solve the problems of low catalytic efficiency, low raw material conversion rate, and the problems of the obtained product after treatment, such as the presence of catalyst residues, flocculent turbid substances in the obtained product, and the significant influence on product quality. The technical scheme comprises the following steps: taking adipic acid and monohydric alcohol as raw materials, fully reacting in a reactor filled with an organotin salt supported catalyst to obtain crude dibutyl adipate, and then performing vacuum distillation, adsorption impurity removal and rectification stripping on the crude dibutyl adipate to obtain dibutyl adipate product, wherein the monohydric alcohol is n-butanol or isobutanol. The preparation method has the advantages of high raw material conversion rate, fast reaction rate, high product yield, product purity ≥ 99.5% after refining treatment, low impurity content, and compliance with the cosmetic product standard, and can be applied to cosmetics.

This invention belongs to the field of bioengineering technology, specifically relating to a BmoR protein mutant capable of sensitively detecting n-butanol or isobutanol, and its application in n-butanol or isobutanol detection or biosensors. The BmoR protein mutant is obtained by mutating the wild-type BmoR protein shown in SEQ ID NO.1 to the E54G mutation. The E54G mutant, possessing the amino acid sequence shown in SEQ ID NO.3, is more sensitive to n-butanol (2.64 × 10⁻⁶). ‑6 mM) and isobutanol (2.16×10 ‑6 The method uses mM) detection to solve the problem of low sensitivity of wild-type BmoR protein, which cannot screen for extremely low concentrations of n-butanol or isobutanol. It can be used for screening and application of strains that produce low levels of higher alcohols.

Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions.

This invention provides an apparatus and method for producing isobutyl methacrylate using a novel membrane reactor. The apparatus integrates a tubular fixed-bed reactor with a pervaporation membrane system. A compartmentalized structure maintains the pressure difference across the membrane, and a dual-tube module optimizes the channel spacing to suppress polymerization side reactions. During the reaction, the pervaporation membrane selectively removes the byproduct water, breaking the equilibrium limitation of the esterification reaction and significantly improving the feed conversion rate. Simultaneously, it disrupts the water-isobutanol azeotropic system, reducing subsequent separation energy consumption and achieving integrated reaction and separation processes. This invention solves the problems of equilibrium limitation on conversion rate, high separation energy consumption, and easy polymerization in traditional processes. It features high conversion rate, low energy consumption, and stable operation, making it suitable for the continuous and green production of isobutyl methacrylate.

An injectable pharmaceutical composition comprising a solution of 3-acyl-buprenorphine or a pharmaceutical salt thereof in a biocompatible organic solvent, wherein the injectable pharmaceutical composition exhibits a sustained release profile lasting for more than one week when injected into a patient. The acyl group is an alkylcarbonyl group and an alkyl portion of the alkylcarbonyl group is straight chain, branched chain, having 1-20 carbon atoms. The biocompatible organic solvent is N-methyl-2-pyrrolidone, ethyl acetate, ethanol, butanol, 2-butanol, isobutanol, isopropanol, glycerin, benzyl benzoate, dimethyl sulfoxide, N,N-dimethylacetamide, propylene glycol, dimethyl glycol, benzyl alcohol, or a combination of two or more thereof.

A process for preparing 2,2,4,4-tetramethylcyclobutane-1,3-diol by reacting 2,2,4,4-tetramethylcyclobutanedione with isobutanol in the presence of a tandem transfer hydrogenation and Tischenko reaction catalyst.

The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.

The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.

The invention provides a method for measuring the content of isobutanol in baijiu, and belongs to the technical field of baijiu quality evaluation. The method for determining the content of isobutanol in Baijiu comprises the following steps: (1) preparing isobutanol standard solutions with different mass concentrations and carrying out gas chromatographic analysis to obtain a peak area corresponding to each isobutanol standard solution, drawing a standard curve by taking the mass concentrations of the plurality of isobutanol standard solutions as abscissas and taking the peak areas corresponding to the plurality of isobutanol standard solutions as ordinates; and (2) performing gas chromatographic analysis on the white spirit by adopting an external standard method to obtain a peak area corresponding to the isobutanol in the white spirit, and then obtaining the content of the isobutanol in the white spirit according to the peak area and the standard curve. According to the determination method, the capillary chromatographic column is utilized to effectively separate the isobutanol in the white spirit, and the isobutanol in the white spirit is quantified by an external standard method, so that the determination method has the advantages of high efficiency and high precision.

Disclosed is a process for preparing 2,2,4,4-tetramethylcyclobutane-1,3-diol by reacting 2,2,4,4-tetramethylcyclobutane dione with isobutanol in the presence of a tandem transfer hydrogenation and Tischenko reaction catalyst.

The invention discloses a complex microbial inoculant for increasing the content of phenethyl alcohol and isobutanol in a liquor fermentation process, and belongs to the technical field of liquor fermentation. According to the invention, pichia kudriavzevii CCTCC NO: M 20252385 is compounded with a plurality of strains of Witzia ciliaris, so that the complex microbial inoculant capable of simultaneously increasing the content of isobutanol and phenethyl alcohol in the fermentation process of Baijiu is obtained. The obtained mixed microbial inoculum can increase the content of isobutanol and phenethyl alcohol, and compared with only using the same amount of pichia kudriavzevii CCTCC NO: M 20252385, the content of isobutanol and the content of phenethyl alcohol are increased by 62.5% and 45.5% respectively.

A process for the manufacture of polyisobutene comprising the steps of:-condensing methanol and a second alcohol in the gas phase, thereby producing an isobutanol-containing reaction mixture,-dehydrating isobutanol to isobutene,-subsequently polymerizing the isobutene thus obtained to polyisobutene.

This invention includes an extractive distillation method for separating isobutanol-isobutyl formate azeotrope using ionic liquids. The process flow is as follows: The mixture of isobutanol and isobutyl formate to be separated is preheated and then enters an extractive distillation column. The ionic liquid 1-butyl-3-methylimidazolium acetate ([BMIM][OAC]) enters from the top of the column. High-purity isobutyl formate product is collected from the top of the column, and the mixture of isobutanol and ionic liquid is collected from the bottom of the column. The mixture is subjected to a two-stage flash distillation process to recover the ionic liquid. The vapor streams from the two flash distillations are condensed to obtain the isobutanol product. The ionic liquid regenerated liquid obtained from the bottom of the second flash distillation is introduced from the top of the extractive distillation column as a circulating stream. This process features high product purity, low energy consumption, high thermal efficiency, and environmental friendliness. Furthermore, it requires less equipment, is simple to operate, and is easily applicable to industrial production.

The invention discloses a method for preparing crystalline L-carnitine, which comprises the step of crystallizing L-carnitine in a mixed solvent consisting of cyclohexane and alcohol, and the alcohol is selected from one or more of n-propanol, isobutanol and n-butanol.

The invention relates to a method for producing a low carbon intensity oil comprising the steps of: a. providing a feed mixture comprising a carbonaceous material and an organic solvent selected from methanol, ethanol, propanol, isopropanol, buthanol, isobuthanol, pentanol, hexanol butanediol, benzene diol, benzene triol, glycerol, ethylene glycol, polyethylene glycol, propylene glycol, acetone, methyl ethyl ketone (MEK), toluene, benzene, hydroxy methyl furfural (HMF), phenols, alkoxyphenols, alkylphenols, creosol, vanillin, formic acid, acetic acid, citric acid or a combination thereof in a concentration of at least 5 % by weight; b. converting the feed mixture at pressures in the range 20 bar to 150 bar and at temperatures in the range 250 °C to 430 °C thereby producing a converted feed mixture; c. passing the converted feed mixture to a first separator operating at pressures in the range 20 to 150 bar and temperatures in the range 250 to 430 °C thereby producing a first gas stream and a first liquid stream; d. cooling the first gas stream to temperature in the range 120 °C to 180 °C and feeding it to a second separator operating at a pressure in the range 20 to 80 bar thereby producing a second gas stream with a reduced content of condensable and second liquid stream comprising recovered condensables; e. cooling the first liquid stream to a temperature in the range 150 °C to 300 °C and feeding it to a third separator operating at a pressure of 1 to 20 bar thereby producing a third gas stream and third liquid stream comprising the crude low carbon intensity oil with a minimum moisture content and a fourth liquid stream comprising water and optionally suspended solids.

The application belongs to the technical field of bioengineering and particularly relates to a BmoR protein mutant with specific response to isobutanol and application of the BmoR protein mutant in isobutanol detection or biosensor. The BmoR protein mutant is obtained by S240P mutation on the basis of wild-type BmoR protein shown in the sequence table SEQ ID NO. 1 and has the amino acid sequence S240P mutant shown in SEQ ID NO. 3. The S240P mutant is only sensitive to isobutanol and has no response to n-butanol, thereby solving the problem that the wild-type BmoR protein cannot distinguish n-butanol and isobutanol high-yield strains. Meanwhile, the detection range of the S240P mutant to isobutanol reaches 0-100 mM, the saturation of the BmoR protein to isobutanol is improved, and the S240P mutant can be used for screening and application of higher-yield strains.

The invention discloses a double-load alkaline gelatin developing microsphere as well as a preparation method and application thereof, and belongs to the technical field of biomedical engineering. The method comprises the following steps: dispersing sodium bicarbonate nanoparticles and barium sulfate in a hot gelatin solution, extruding into drops through an injection pump, dropwise adding the drops into liquid paraffin which is stirred at a low temperature, freezing and forming, crosslinking and curing at a low temperature through a glutaraldehyde isobutanol solution, and washing and drying to obtain a final product. The double-loaded alkaline gelatin developing microspheres obtained by the invention are uniform in particle size (300-500 [mu] m) and good in dispersity, barium sulfate endows the double-loaded alkaline gelatin developing microspheres with stable and lasting X-ray developing capacity, and the entrapped sodium bicarbonate nanoparticles can slowly release alkaline substances, effectively neutralize lactic acid in a tumor microenvironment and reverse chemotherapy drug resistance caused by the alkaline substances. The microsphere provided by the invention is simple and controllable in preparation process, can be used as an embolic agent with developing guiding and drug resistance reversing functions, and is used for tumor interventional therapy.

This invention belongs to the field of bioengineering technology, specifically relating to a BmoR protein mutant capable of sensitively detecting n-butanol or isobutanol, and its application in n-butanol or isobutanol detection or biosensors. The BmoR protein mutant is obtained by mutating the wild-type BmoR protein shown in SEQ ID NO.1 to V311A. The V311A mutant, possessing the amino acid sequence shown in SEQ ID NO.3, is more sensitive to n-butanol (2.13 × 10⁻⁶). ‑6 mM) and isobutanol (1.94×10) ‑6 The method uses mM) detection to solve the problem of low sensitivity of wild-type BmoR protein, which cannot screen for extremely low concentrations of n-butanol or isobutanol. It can be used for screening and application of strains that produce low levels of n-butanol or isobutanol.

This invention discloses a method for preparing 3,5,5-trimethylhexanoic acid and its application, belonging to the technical field of 3,5,5-trimethylhexanoic acid preparation. The method for preparing 3,5,5-trimethylhexanoic acid includes the following steps: S1, dehydrating tert-butanol to prepare isobutylene; wherein the tert-butanol used contains <0.3% by mass of n-propanol, <0.3% by mass of isopropanol, <0.3% by mass of n-butanol, and <0.3% by mass of isobutanol; S2, preparing diisobutylene from the isobutylene obtained in S1; S3, preparing 3,5,5-trimethylhexanal from the diisobutylene obtained in S2 via hydroformylation; S4, preparing 3,5,5-trimethylhexanal from the 3,5,5-trimethylhexanal obtained in S3 via oxidation. The 3,5,5-trimethylhexanoic acid obtained by this invention has high purity.

The invention relates to an environment-friendly antistatic agent for a TPEE material and a preparation method of the environment-friendly antistatic agent, and belongs to the technical field of high polymer material additives. The antistatic agent comprises 62%-68% of isobutanol, 6%-9% of N-methyl pyrrolidone, 8%-12% of antistatic active component long-chain alkyl quaternary ammonium salt, 8%-11% of polyethylene glycol, 8%-11% of zinc acetate and 0.2%-0.5% of edible essence. Through reasonable proportioning of the components, the antistatic agent and the TPEE material have good compatibility, the surface resistance of the TPEE material can be effectively reduced, the TPEE material is endowed with lasting and stable antistatic performance, meanwhile, the odor of the material is improved, and the antistatic agent is simple in preparation process, controllable in cost and suitable for industrial production.

The application belongs to the technical field of bioengineering, and particularly relates to a BmoR protein mutant with a wider detection range (0-200 mM) for n-butanol or isobutanol and application of the BmoR protein mutant in detection of n-butanol or isobutanol or biosensors. The BmoR protein mutant is obtained by T12N mutation on the basis of a wild-type BmoR protein shown in the sequence table SEQ ID NO. 1, and has an amino acid sequence T12N mutant shown in SEQ ID NO. 3. The T12N mutant has a wider detection range (0-200 mM) for n-butanol or isobutanol, solves the problem that the wild-type BmoR protein cannot screen n-butanol or isobutanol high-yield strains, and can be used for screening and application of higher-yield strains.

This invention belongs to the field of microbial fermentation technology, specifically relating to a high-yield isobutanol-producing Jejubacterium chassis strain, and a method for obtaining an isobutanol-producing strain and fermenting to produce isobutanol using this strain. This invention obtains Jejubacterium DT01 IM3 DL3 (CGMCC No. 30883) through heavy ion beam mutagenesis combined with MP6 plasmid-mediated random mutagenesis screening. The isobutanol-producing strain constructed using Jejubacterium DT01 IM3 DL3 as the chassis strain achieves an isobutanol yield of 11.9 g / L in shake-flask fermentation, and a super-high yield of 148 g / L was achieved in a 3L fermenter scale-up experiment. Furthermore, fermentation can be carried out at different salt concentrations (10-60 g / L), providing a possibility for industrial production using high-salt or open fermentation processes.

This invention discloses a catalyst for the synthesis of isobutanol from syngas, its preparation method, and its application, belonging to the technical field of catalysts for chemical products. The catalyst consists of a composite support, an active component of Cu oxide, and an alkali metal oxide promoter; the support is a high specific surface area ZrO2-SiO2 support and a ZnCrO2 support. x A mixture of carriers; the active component is CuO; the alkali metal auxiliaries are either K2O or Cs2O; the weight percentages of each component are as follows: ZrO2-SiO2 composite carrier: 28.69%~37.70%; ZnCrO x Composite support: 53.80%~65.54%; Active component CuO: 2.95%~6.13%; Alkali metal oxide auxiliaries: 2.58%~5.87%. The catalyst provided by this invention can efficiently synthesize isobutanol at relatively low reaction temperatures and pressures. Methanol and isobutanol are the main alcohol products, accounting for over 96% of the total alcohols, with other alcohols being less abundant. Subsequent separation consumes low energy. When the catalyst of this invention is applied to the direct synthesis of isobutanol from catalytic syngas, the CO conversion rate is greater than 60%, and the selectivity for isobutanol in the products reaches up to 47.6%, demonstrating significant potential for industrial application.