909 results about "Isobutanol" patented technology

Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.

A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudoroff pathway.

A method for the production of isobutanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

The invention relates to suitable candidate ADH enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.

An E. coli host strain was engineered wherein genes adhE, IdhA, frdB, and pfIB were disrupted and novel butanol dehydrogenase gene, sadB, from Achromobacter xylosoxidans, was added to produce the isobutanol production host.

The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Compositions and lures are described which provide vapor blends of acetic acid and one or more compounds selected from the group consisting of isobutanol, racemic 2-methyl-1-butanol, S-(-)-2-methyl-1-butanol, 2-methyl-2-propanol, heptyl butyrate, and butyl butyrate which function as highly effective attractants for yellowjacket wasps and paper wasps. By attracting wasps to traps or baits, the chemical attractants provide a means for detecting, surveying, monitoring, and controlling the wasps.

Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed.

A high-content environmental-protecting alcohol ether fuel for vehicle consists of additives and methanol or alcohol 75-87 proportion. The additives contain methanol, alcohol, isopropyl alcohol, isobutanol, dimethylcuprate, n-amyl alkyl, dipropyl, cyclopentane, nitro-methane, dimethyl benzene, pinecone oil, cyclopentadienyl, poly-isobutylene, propane diamine, carbomethoxy formate, dimethoxyester carbonate, natural gasoline, hydrocarbon solvent oil, diisopropyl ester, dimethoxy-methane, petroleum ether, methyl tert-amyl ether, tert-amyl tert-butyl ether, lauryl phenol poly-ethenoxy ether, anti-static agent, metal-corrosive inhibitor, rubber plastic swelling inhibitor and anti-oxidant. It's clean and universal; it has excellent burning stability, shock resistance and dynamic character and less oil consumption.

The invention relates generally to the field of industrial microbiology and butanol production from sources of 5-carbon sugars such as lignocellulosic hydrolysates. More specifically, the invention relates to the use of an xylulose or xylulose-5-phosphate-producing enzyme and micro-aerobic or anaerobic conditions to increase butanol production from such sugars and recovery of said butanol through ins situ product recovery methods.

The invention discloses a plastic paint with simulated electroplating effect. The composition and the weight ratio of the invention are as follows: 30 to 45 of acrylic resin with 50 percent of solid content and 70 to 90 DEG C of Tg, 20 to 30 of cellulose acetate butyrate with 20 percent of solid content, 8 to 12 of triad copolymer vinyl chloride-acetate resin with 30 percent of solid content, 6 to 10 of non-floating aluminum and silver pulp with an average diameter less than or equal to 20 Mum, 0.3 to 0.5 of dispersant, 0.2 to 0.4 of flatting agent, 0.4 to 0.6 anti-settling agent, 6.0 to 10.0 of toluene, 3.5 to 6.0 of ethyl acetate, 3.5 to 6.0 methy isobutyl ketone, 3.0 to 5.0 of isobutanol and 4.0 to 7.0 of glycol butyl ether. The special diluent of the invention, according to the weight ratio, is made from the following components evenly mixed: 20 of white gas, 10 of toluene, 22 of ethyl acetate, 15 of acetone, 25 of isobutanol and 8 of glycol butyl ether. The main paint which comprises the composition and the diluent are evenly mixed according to the ratio of 1: 2-3, then the mixture is used for spray coating; the metallic appearance of the paint film obtained is very close to the effect of the electroplating, and the paint film has good alcohol resistance with high hardness and strong wear resistance, and also has simple process. The aluminum and silver pulp used is a common type, the cost of which is only 5 percent to 10 percent of the simulated electroplating aluminum and silver pulp and about 2 percent of electrosilvering.

The invention discloses vehicle high-methanol content gasoline, relates to carbon-containing fuels and solves the problems of difficult low-temperature startup, serious metal corrosion, inadequate power and susceptibility to generating precipitates of the conventional methanol gasoline. The vehicle high-methanol content gasoline comprises the following components in part by volume: 70 to 86 partsof methanol, 14 to 30 parts of gasoline component oil, 0.5 to 1 part of cosolvent, 0.1 to 0.2 part of antiseptic and rubber swelling inhibitor, 0.2 to 0.3 part of detergent and 0.01 to 0.05 part of coloring agent. The cosolvent consists of isobutanol, isopropyl ether, 2-isobutanol and tetrahydrofuran, the antiseptic and rubber swelling inhibitor consists of propargyl alcohol, butanedioic acid, hexakis(dihydrogen phosphate), N-methyl morpholine, cyclohexane and dimethyl phthalate, the detergent consists of normal propyl alcohol, isopropanol, limonene and cyclohexane, and the coloring agent is Sudan IV solution. The vehicle high-methanol content gasoline has high startability, high dynamic performance, high environmental protection performance, obvious anticorrosion effect and high safety.

The present invention relates to a process for the production of propylene in which in a first step isobutanol is subjected to a simultaneous dehydration and skeletal isomerisation to make substantially corresponding olefins, having the same number of carbons and consisting essentially of a mixture of n-butenes and iso-butene and in a second step n-butenes are subjected to methathesis, said process comprising:

• a) introducing in a reactor a stream (A) comprising isobutanol, optionally water, optionally an inert component,
• b) contacting said stream with a catalyst in said reactor at conditions effective to dehydrate and skeletal isomerase at least a portion of the isobutanol to make a mixture of n-butenes and iso-butene,
• c) recovering from said reactor a stream (B), removing water, the inert component if any and unconverted isobutanol if any to get a mixture of n-butenes and iso-butene,
• d) fractionating said mixture to produce a n-butenes stream (N) and to remove the essential part of isobutene optionally recycled with stream (A) to the dehydration/isomerization reactor of step b),
• e) sending the stream (N) to a methathesis reactor and contacting stream (N) with a catalyst in said methathesis reactor, optionally in the presence of ethylene, at conditions effective to produce propylene,
• f) recovering from said methathesis reactor a stream (P) comprising essentially propylene, unreacted n-butenes, heavies, optionally unreacted ethylene,
• g) fractionating stream (P) to recover propylene and optionally recycling unreacted n-butenes and unreacted ethylene to the methathesis reactor.

The invention relates to the field of power supply materials, and discloses a high and low voltage power distribution cabinet housing material. The material is prepared from the raw materials of, by weight, 30-40 parts of polyvinyl chloride, 20-25 parts of silicon carbide, 10-12 parts of borax, 8-10 parts of quartz sand, 8-10 parts of phenolic resin, 8-9 parts of glycerol monostearate, 7-8 parts of isobutanol, 7-8 parts of polycarbonate, 5-7 parts of diethylene glycol monobutyl ether, 5-7 parts of decabromodiphenyl ethane, 2-3 parts of graphite, 2-3 parts of aluminum tripolyphosphate, 2-3 parts of antimony trioxide, 1-2 parts of glass fiber, and 1-2 parts of silica gel powder. According to the housing material provided by the invention, good insulation performance can be effectively maintained. Mechanical performance, heat conduction performance, and flame retardant performance of the material are greatly improved.

The invention relates to a method for hydrogenation preparing isobutyraldehyde and isobutyl acohol from methacrolein. The method comprises using a supported metal catalyst, and hydrogenating methacrolein obtained by oxidating isobutene under gentle conditions. The invention provides a novel synthesis process for hydrogenation preparing isobutyraldehyde and isobutyl acohol from methacrolein which is obtained by air-oxidating low-utilization C4 material isobutene or tert-butanol. The methacrolein conversion rate is larger than 98%, the total selectivity of isobutyraldehyde and isobutyl acohol is larger than 95%, the performance of the catalyst is stable even the reaction lasts more than 1000 hours. The invention has the advantages of high activity and high selectivity of catalyst, good stability of catalyst, cheap material isobutene, low productin cost of isobutene, environment-protecting process, and suitability of industrialisation production. The process is flexible, can change the proportion of isobutyraldehyde and isobutyl acohol through adjusting the reaction process condition, thereby cogenerating isobutyraldehyde and isobutyl acohol, or only generating isobutyl acohol by cycling and hydrogenating isobutyraldehyde.

The invention is a silicotungstic and phosphotungstic heteropoly acid load catalyst used for esterification reaction, which comprises the following components: Keggin structure silicotungstic acid H3SiW12O40.xH2O and phosphotungstic H3PW12O40.xH20, the content occupies 10 to 50 quality per centage of the amount of the catalyst; the carrier is H Beta zeolite. The invention adopts soaking method to prepare the solid silicotungstic and phosphotungstic heteropoly acid catalyst load on the H Beta zeolite, which can be used for catalyzing the esterification reaction of etic acid, isobutanol, isobutanol and ethanol, but also can be used for catalyzing esterification reaction of lactic acid and isobutanol to synthesis n-butyl acetate, isobutyl acetate, ethyl acetate and N-Butyl Lactate. The invention has the advantages of good stability, high activity and good selectivity, but also can used as inhomogeneous catalyst which is easy to be separated with reaction product and can use the prepared and formed catalyst powder in the continuous operating fixed bed reactor. The invention is used in the techinics of esterification reaction catalyzing rectifying.

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.