The invention discloses a construction method of
engineering bacteria for producing
glycerol and lipid by efficiently fermenting lignocellulose
hydrolysate, and belongs to the technical field of microorganisms. The invention provides a method for producing
glycerol and lipid by fermenting lignocellulose
hydrolysate by using Candida glycinogenes which is resistant to high
osmotic pressure as a production strain. According to the present invention, with the Candida glycyinogenes
engineering strain Cg-AAGD constructed by using the method, the
virus-free lignocellulose
hydrolysate (containing about 175 g / L of glucose and 25 g / L of
xylose) can be fermented to produce 82.2 g / L of
glycerol and 7.3 g / L of lipid; according to the method, the lignocellulose hydrolysate is selected as a
raw material, the purpose of deep
resource utilization of
agricultural waste is achieved, the related technological operation flow is relatively simple and convenient, and the requirement for production equipment is easy to achieve.