10 results about "Lignocellulosic hydrolysates" patented technology

The present invention discloses a method for degrading plant biomass of agricultural and forestry waste and its application. In the present invention, plant biomass of agricultural and forestry waste, trivalent iron minerals and nutrient solution are mixed, and a hydrolysis microbial bacterial liquid is selectively added, and the obtained reaction system A is anaerobically cultured to obtain a hydrolysis solution. The hydrolysis solution contains hydrolysis products of lignocellulose and has a low chromaticity, and can be used as a denitrification carbon source. This method has low energy consumption and cost, and can significantly improve the carbon source utilization rate of lignocellulose; the obtained hydrolysis solution has a low chromaticity and high denitrification potential, and can be used as a liquid carbon source to supply sewage treatment plants for use as an external denitrification carbon source. It can be seen that this method can not only effectively degrade plant biomass of agricultural and forestry waste, but also the obtained hydrolysis solution can be used as a denitrification carbon source for sewage treatment.

The invention discloses a construction method of engineering bacteria for producing glycerol and lipid by efficiently fermenting lignocellulose hydrolysate, and belongs to the technical field of microorganisms. The invention provides a method for producing glycerol and lipid by fermenting lignocellulose hydrolysate by using Candida glycinogenes which is resistant to high osmotic pressure as a production strain. According to the present invention, with the Candida glycyinogenes engineering strain Cg-AAGD constructed by using the method, the virus-free lignocellulose hydrolysate (containing about 175 g / L of glucose and 25 g / L of xylose) can be fermented to produce 82.2 g / L of glycerol and 7.3 g / L of lipid; according to the method, the lignocellulose hydrolysate is selected as a raw material, the purpose of deep resource utilization of agricultural waste is achieved, the related technological operation flow is relatively simple and convenient, and the requirement for production equipment is easy to achieve.

Methods and compositions (e.g., engineered hosts) are disclosed for use in converting biomass to 3-hydropropionic acid. In particular embodiments, the methods include use of an engineered Rhodosporidium yeast, such as R. toruloides, the engineered R. toruloides having the RT04_8975 gene deleted from its genome, combined with a lignocellulosic hydrolysate, sourced, for example, from a biomass. A promoter for enhancing transport of 3HP is also incorporated by addition to the R. toruloides genome, for example, by modified lithium acetate transformation.

The invention discloses a method for controlling the growth of bacterial strain concentration, and belongs to the field of lactic acid fermentation preparation. The method comprises the following steps: preparing a culture medium, adding culture medium raw materials and sterile water; the culture medium comprises: 60-65 g / L of glucose; 15 g / L of lignocellulose hydrolysate; 5-6 g / L of yeast extract; 10-12 g / L of peptone; 1-1.5 g / L of buffer; adjusting the pH value of the culture medium to 5.5-6.0; transferring the Pichia seed liquid to the fermentation tank, and then adding the culture medium to the fermentation tank to maintain the fermentation environment at 30-35 degrees Celsius; when the bacterial strain concentration exceeds the threshold value and the lactic acid production decreases, the fermentation temperature is increased by 3 degrees Celsius to inhibit the bacterial strain concentration.

The present invention lies in the field of microbial fatty acid / lipid / oil production from lignocellulosic feedstock / material / biomass, in particular from lignocellulose degradation products like lignocellulose hydrolysates. The present invention relates to a method of producing an oleaginous yeast strain adapted to a lignocellulose medium. The present invention further relates to an oleaginous yeast strain adapted to a lignocellulose medium and produced by the method of the invention and to an oleaginous yeast strain adapted to a lignocellulose medium, said strain being characterized by a particular RNA expression profile (showing, for example, an activated metabolic stress response RPOS). The present invention further relates to a method of producing oleaginous yeast cells, to a method of producing fatty acids and / or lipids, and to a method of producing (vegan) nutraceuticals / nutritional supplements or petroleum replacers. The present invention further relates to microbial methods for conversion of lignocellulosic hydrolysates using oleaginous yeast, in particular for producing oleaginous yeast cells, fatty acids, lipids, oils, (vegan) nutraceuticals / nutritional supplements and / or petroleum replacers.

The present invention provides a recombinant expression transformant tolerant to furfural and its application. These furfural-tolerant recombinant expression transformants respectively contain genes carrying mutation sites, and these genes include PP_RS18130<supgt;T65A / A67G< / supgt>, PP_RS02880<supgt;T517A< / supgt>, PP_RS19785<supgt;C20A< / supgt>, PP_RS20740<supgt;G1012T< / supgt>, PP_RS02385<supgt;A1046G< / supgt>. The recombinant expression transformants containing these genes are applied to the detoxification of pretreated lignocellulosic hydrolysate, which can effectively reduce the inhibitory effect of toxic by-products in the hydrolysate on fermentation microorganisms, thereby improving the efficient production of lignocellulosic raw materials into bio-based products. The present invention also provides a preparation method for these recombinant expression transformants, including the construction of a recombinant expression vector, the transformation of the expression vector, and the toxicity tolerance and transformation ability of the recombinant expression transformant to furfural.

This invention discloses a method for detoxifying resin from regenerated lignocellulosic hydrolysate, belonging to the field of ionomer resin regeneration technology. The method first detoxifies the lignocellulosic hydrolysate with resin, then separates the detoxified hydrolysate from the poisoned resin, and finally regenerates the poisoned resin using a regenerating agent, namely hydrogen peroxide and sodium hydroxide, for reuse. This invention restores the adsorption efficiency of the regenerated resin for acetic acid and identifiable total inhibitors to 86.97% and 80.49%, respectively, without damaging the basic resin framework and functional groups. Furthermore, the resin can be recycled three times, significantly improving the overall economic and environmental benefits of the process.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

An integrated process for producing polyhydroxyalkanoates and bioethanol including:(a) feeding a part of the lignocellulosic hydrolyzate to a first fermentation device in the presence of one microorganism capable of using sugars with six carbon atoms and organic acids, obtaining a first fermentation broth;(b) subjecting the first broth to separation obtaining an aqueous suspension of cellular biomass having one polyhydroxyalkanoate and an aqueous phase having sugars with five carbon atoms in a quantity greater than or equal to 10 g / L;(c) optionally, feeding a part of the aqueous phase from step (b), to a second fermentation device, obtaining a second fermentation broth (inoculum);(d) feeding at least a part of the aqueous phase from step (b) and, optionally, the second broth and / or at least a part of lignocellulosic hydrolyzate, to a third fermentation device, obtaining a third fermentation broth; and(e) subjecting the third broth to separation obtaining bioethanol.