Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product

A technology of lignocellulose and hydrolyzate, which is applied in the field of in-situ detoxification ethanol fermentation, can solve the problems such as the complexity and cost of ethanol fermentation process, and achieve the effects of good mixed fermentation compatibility, convenient operation and fast fermentation speed

Inactive Publication Date: 2009-02-04
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrolyzate is usually detoxified before fermentation, which requires the addition of treatment equipment, and the detoxification equipment must be well integrated with the fermentation process, thus increasing the complexity of the entire ethanol fermentation process and increasing the production cost

Method used

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  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product
  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product
  • Virus-free in situ and alcohol fermentation method of composite bacteria for lignocellulose hydrolysis product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 In-situ detoxification ethanol fermentation of lignocellulose hydrolyzate by composite bacteria of the present invention

[0039] The strains S.cerevisiaeY5 (Y5) and I.orientalis Y4 (Y4) were screened and preserved by our laboratory. The strain P.stipitis CBS6054 (CBS6054) was purchased from ATCC, USA.

[0040] Preparation of proliferation medium: glucose 20g / L, yeast extract 10g / L, peptone 20g / L, pH5.0-5.5, sterilized at 121°C for 20min.

[0041] Preparation of fermentation medium: yeast extract 10g / L, peptone 20g / L, lignocellulose dilute acid hydrolyzate 100ml (wherein glucose is 15.4g / L, xylose 6.6g / L, furfural 1.37g / L, 5-HMF0 .47g / L, acetic acid 10g / LCa(OH) 2 Adjust the pH of the hydrolyzate to 5.0), and sterilize at 115°C for 30min.

[0042] Inoculate strains Y5 and CBS6054 into 100ml proliferation medium, culture at 30°C and 150rpm for 12-14h, and the viable counts of the obtained strains were 10 7 cfu / L, 10 8 cfu / L.

[0043] Inoculate 5ml of CBS60...

experiment example 1

[0044] Ethanol fermentation of experimental example 1 single strain Y5, Y4, CBS6054

[0045] 1. Test material

[0046] The strains S.cerevisiaeY5 (Y5) and I.orientalis Y4 (Y4) were screened and preserved by our laboratory. The strain P.stipitis CBS6054 (CBS6054) was purchased from ATCC, USA.

[0047] 2. Test method

[0048]The bacterial strains S.cerevisiaeY5, I.orientalisY4 and P.stipitisCBS6054 were respectively inoculated in 100ml of the proliferation medium in Example 1, cultured at 30°C and 150rpm for 12h, and the dry cell weights of the obtained strains were 2.7g / L and 3.0g / L respectively. g / L, 3.3g / L.

[0049] Take 5ml each of Y5, Y4 and CBS6054 bacteria solution and inoculate them into 100ml of glucose, glucose+, xylose, xylose+, mixed sugar, mixed sugar+ (the concentrations of glucose and xylose are 24g / l and 13g / l respectively) In the liquid culture medium, "+" means that furfural 1.37g / L and 5-HMF0.47g / L were added, cultured at 30°C and 80rpm, and the concentrat...

experiment example 2

[0064] Experimental Example 2 Mixed sugar ethanol fermentation of mixed strains Y5+CBS6054 and Y4+CBS6054

[0065] 1. Test material

[0066] Bacteria and proliferation medium are the same as in Experimental Example 1.

[0067] Preparation of fermentation medium: yeast extract 10g / L, peptone 20g / L, glucose 24g / L, xylose 13g / L, furfural 1.37g / L, 5-HMF0.47g / L, adjust the pH of the hydrolyzate with calcium hydroxide To 5.0, sterilize at 115°C for 30min.

[0068] 2. Test method

[0069] 5ml of CBS6054 and Y5 seed solutions were inoculated in 100ml fermentation medium, 5ml of CBS6054 and Y4 seed solutions were inoculated in 100ml fermentation medium, cultured at 30°C and 80rpm, and the concentrations of ethanol, sugar, furfural and 5-HMF were measured .

[0070] 3. Analytical method (with experimental example 1)

[0071] 4. Calculation method (same as experimental example 1)

[0072] 5. Test results

[0073] (1) Mixed sugar ethanol fermentation of compound bacteria Y5+CBS6054...

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PUM

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Abstract

The present invention relates to an in-situ detoxification ethanol fermentation method of lignocellulose hydrolysate, which respectively inoculates Y5 and CBS6054 into fermentation culture medium containing dilute acid lignocellulose hydrolysate to carry out in-situ detoxification alcoholic fermentation. The method can efficiently metabolize glucose and xylose in the dilute acid lignocellulose hydrolysate to produce ethanol, and rapidly metabolize furfural and 5-hydroxymethylfurfural. The yield of ethanol is 0.44g / g, reaching 87.5 percent of the theoretical value. Moreover, any detoxification processing is not needed, the requirement on equipment is low, the operation is convenient, and the cost is low.

Description

technical field [0001] The invention relates to an in-situ detoxification ethanol fermentation method of lignocellulose hydrolyzate, in particular, a method that can efficiently metabolize glucose and xylose in dilute acid hydrolyzate of lignocellulose to produce ethanol and rapidly metabolize inhibitory factor The complex bacteria of furfural and 5-hydroxymethylfurfural were inoculated into the fermentation medium containing lignocellulose dilute acid hydrolyzate for in-situ detoxification ethanol fermentation. Background technique [0002] In the field of research and development of ethanol production using lignocellulose as raw material, dilute acid hydrolysis of lignocellulose is considered to be the most easily commercialized production process, and a lot of in-depth research has been carried out in this field at home and abroad. But so far, there are still some problems in dilute acid hydrolysis of lignocellulose to produce fuel ethanol at home and abroad. One of the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/10C12R1/865C12R1/84
CPCY02E50/16Y02E50/10
Inventor 杨秀山
Owner CAPITAL NORMAL UNIVERSITY
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